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FR2726574B1 - Procede de dissociation des cellules eucaryotes en culture - Google Patents

Procede de dissociation des cellules eucaryotes en culture

Info

Publication number
FR2726574B1
FR2726574B1 FR9413249A FR9413249A FR2726574B1 FR 2726574 B1 FR2726574 B1 FR 2726574B1 FR 9413249 A FR9413249 A FR 9413249A FR 9413249 A FR9413249 A FR 9413249A FR 2726574 B1 FR2726574 B1 FR 2726574B1
Authority
FR
France
Prior art keywords
dissociation
culture
eukaryotic cells
eukaryotic
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9413249A
Other languages
English (en)
Other versions
FR2726574A1 (fr
Inventor
Jean Loup Salzmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite Pierre et Marie Curie
Original Assignee
Universite Pierre et Marie Curie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite Pierre et Marie Curie filed Critical Universite Pierre et Marie Curie
Priority to FR9413249A priority Critical patent/FR2726574B1/fr
Priority to PCT/FR1995/001435 priority patent/WO1996014395A1/fr
Priority to US08/836,596 priority patent/US5906939A/en
Priority to JP8515089A priority patent/JPH10510144A/ja
Priority to EP95939309A priority patent/EP0789756A1/fr
Publication of FR2726574A1 publication Critical patent/FR2726574A1/fr
Application granted granted Critical
Publication of FR2726574B1 publication Critical patent/FR2726574B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FR9413249A 1994-11-04 1994-11-04 Procede de dissociation des cellules eucaryotes en culture Expired - Fee Related FR2726574B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR9413249A FR2726574B1 (fr) 1994-11-04 1994-11-04 Procede de dissociation des cellules eucaryotes en culture
PCT/FR1995/001435 WO1996014395A1 (fr) 1994-11-04 1995-10-31 Procede de dissociation des cellules eucaryotes en culture
US08/836,596 US5906939A (en) 1994-11-04 1995-10-31 Cultured fibroblast or epithelial cell dissociation method using sulfated polysaccharide and chelator
JP8515089A JPH10510144A (ja) 1994-11-04 1995-10-31 培養真核細胞の解離方法
EP95939309A EP0789756A1 (fr) 1994-11-04 1995-10-31 Procede de dissociation des cellules eucaryotes en culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9413249A FR2726574B1 (fr) 1994-11-04 1994-11-04 Procede de dissociation des cellules eucaryotes en culture

Publications (2)

Publication Number Publication Date
FR2726574A1 FR2726574A1 (fr) 1996-05-10
FR2726574B1 true FR2726574B1 (fr) 1997-01-17

Family

ID=9468535

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9413249A Expired - Fee Related FR2726574B1 (fr) 1994-11-04 1994-11-04 Procede de dissociation des cellules eucaryotes en culture

Country Status (5)

Country Link
US (1) US5906939A (fr)
EP (1) EP0789756A1 (fr)
JP (1) JPH10510144A (fr)
FR (1) FR2726574B1 (fr)
WO (1) WO1996014395A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2218775B1 (fr) 1996-08-30 2015-01-28 Life Technologies Corporation Méthode de production d'un polypeptide in vitro dans une cellule de mammifère dans un milieu de culture sans sérum and sans protéines
EP1484409B1 (fr) * 1996-12-16 2009-04-08 Eisai R&D Management Co., Ltd. Construction d'AND comprenant un gène de résistance à un médicament et un gène étranger
DE60142506D1 (de) * 2000-03-03 2010-08-19 Chemo Sero Therapeut Res Inst In serumfreier kultur verwendbare zelle, kultursuspension und verfahren zur virusproduktion als impfstoff unter verwendung der zelle
WO2006109367A1 (fr) * 2005-04-05 2006-10-19 Obihiro University Of Agriculture And Veterinary Medicine Méthode de libération cellulaire, solution de libération cellulaire, méthode de culture cellulaire, milieu de culture cellulaire, solution cellulaire, préparation de solution cellulaire, méthode de colonisation cellulaire et solution de colonisation cellulaire

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1204399A (fr) * 1983-11-10 1986-05-13 Elliot M. Levine Procede et milieu pour le clonage et la culture en serie a long terme de cellules endotheliales humaines
US5024947A (en) * 1987-07-24 1991-06-18 Cetus Corporation Serum free media for the growth on insect cells and expression of products thereby
DE3938907C2 (de) * 1989-11-24 1999-11-04 Dade Behring Marburg Gmbh Mittel zum Lagern und Suspendieren von Zellen, insbesondere Erythrozyten
US5348877A (en) * 1993-03-12 1994-09-20 Boyce Thompson Institute For Plant Research, Inc. Method of adapting anchorage-dependent cell lines to suspension conditions

Also Published As

Publication number Publication date
WO1996014395A1 (fr) 1996-05-17
JPH10510144A (ja) 1998-10-06
EP0789756A1 (fr) 1997-08-20
FR2726574A1 (fr) 1996-05-10
US5906939A (en) 1999-05-25

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Legal Events

Date Code Title Description
ST Notification of lapse