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FR2265854A1 - Nucleic acids extraction from yeast - using improved acid hydrolysis process - Google Patents

Nucleic acids extraction from yeast - using improved acid hydrolysis process

Info

Publication number
FR2265854A1
FR2265854A1 FR7509675A FR7509675A FR2265854A1 FR 2265854 A1 FR2265854 A1 FR 2265854A1 FR 7509675 A FR7509675 A FR 7509675A FR 7509675 A FR7509675 A FR 7509675A FR 2265854 A1 FR2265854 A1 FR 2265854A1
Authority
FR
France
Prior art keywords
paste
extruding
yeast
nucleic acids
soln
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7509675A
Other languages
French (fr)
Other versions
FR2265854B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Liquichimica SpA
Original Assignee
Liquichimica SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Liquichimica SpA filed Critical Liquichimica SpA
Publication of FR2265854A1 publication Critical patent/FR2265854A1/en
Application granted granted Critical
Publication of FR2265854B1 publication Critical patent/FR2265854B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Nucleic acids are extd. from yeasts intended for human consumption by acid hydrolysis, the improvement comprising using a cells/HCl soln. ratio of about 1:1. The process consists in introducing the unpressed, hydrocarbon-washed moist yeast cell paste into a vessel, injecting steam at the vessel base, extruding with stirring, via the base, the fluidised paste into an acidification chamber, stirring being effected with a helical stirrer, injecting the HCl soln. pref. at a concn. of 0.15N-1N into the acidification chamber and kneading the paste with twin helical stirrers dispersed axially vertically relative to the first helical stirrer, extruding the paste into an incubation chamber kept at 100 degrees C extruding into a washing tank, recovering the hydrolysed cellular paste and weighing and finally recovering the washing soln. and hydrolysate concentrate. The process ensures complete hydrolysis of the nucleii acids which if present in the yeast for eventual consumption would give rise to formation of uric acid.
FR7509675A 1974-03-29 1975-03-27 Nucleic acids extraction from yeast - using improved acid hydrolysis process Granted FR2265854A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT4988474A IT1018642B (en) 1974-03-29 1974-03-29 PERFECTED PROCESS FOR THE EXTRACTION OF NUCLEIC ACIDS IN YEAST FOR HUMAN FEEDING

Publications (2)

Publication Number Publication Date
FR2265854A1 true FR2265854A1 (en) 1975-10-24
FR2265854B1 FR2265854B1 (en) 1978-09-29

Family

ID=11271778

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7509675A Granted FR2265854A1 (en) 1974-03-29 1975-03-27 Nucleic acids extraction from yeast - using improved acid hydrolysis process

Country Status (6)

Country Link
JP (1) JPS50129795A (en)
BE (1) BE827054A (en)
DE (1) DE2513944A1 (en)
FR (1) FR2265854A1 (en)
IT (1) IT1018642B (en)
NL (1) NL7503648A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL423875A1 (en) * 2017-12-14 2019-06-17 Eugeniusz Foss Method for obtaining protein with decreased content of nucleic acids and protein free from nucleic acids

Also Published As

Publication number Publication date
BE827054A (en) 1975-07-16
FR2265854B1 (en) 1978-09-29
IT1018642B (en) 1977-10-20
NL7503648A (en) 1975-10-01
JPS50129795A (en) 1975-10-14
DE2513944A1 (en) 1975-10-16

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Legal Events

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ST Notification of lapse