ES2869525T3 - Método para producir heparán sulfato que presenta una actividad anticoagulante - Google Patents
Método para producir heparán sulfato que presenta una actividad anticoagulante Download PDFInfo
- Publication number
- ES2869525T3 ES2869525T3 ES16881650T ES16881650T ES2869525T3 ES 2869525 T3 ES2869525 T3 ES 2869525T3 ES 16881650 T ES16881650 T ES 16881650T ES 16881650 T ES16881650 T ES 16881650T ES 2869525 T3 ES2869525 T3 ES 2869525T3
- Authority
- ES
- Spain
- Prior art keywords
- sulfation
- heparosan
- molecular weight
- solution
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
- A61K31/727—Heparin; Heparan
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
- C12Y402/02008—Heparin-sulfate lyase (4.2.2.8)
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Diabetes (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Public Health (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015256988 | 2015-12-28 | ||
PCT/JP2016/087688 WO2017115674A1 (ja) | 2015-12-28 | 2016-12-16 | 抗凝固活性を有するヘパラン硫酸の製造法 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2869525T3 true ES2869525T3 (es) | 2021-10-25 |
Family
ID=59225768
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES16881650T Active ES2869525T3 (es) | 2015-12-28 | 2016-12-16 | Método para producir heparán sulfato que presenta una actividad anticoagulante |
Country Status (6)
Country | Link |
---|---|
US (1) | US10704068B2 (de) |
EP (1) | EP3399044B1 (de) |
JP (1) | JP6879216B2 (de) |
ES (1) | ES2869525T3 (de) |
HU (1) | HUE053923T2 (de) |
WO (1) | WO2017115674A1 (de) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017115674A1 (ja) * | 2015-12-28 | 2017-07-06 | 味の素株式会社 | 抗凝固活性を有するヘパラン硫酸の製造法 |
WO2018135027A1 (ja) | 2017-01-19 | 2018-07-26 | 味の素株式会社 | ヘキスロン酸残基が異性化されたヘパロサン化合物の製造方法 |
EP4050036A1 (de) * | 2017-09-05 | 2022-08-31 | Ajinomoto Co., Inc. | 3-o-sulfatierungsenzymmutante sowie verfahren zu ihrer verwendung |
CN109321508B (zh) * | 2018-10-12 | 2022-12-27 | 北京化工大学 | 产heparosan的基因工程菌及其应用 |
CN113646427A (zh) | 2019-01-15 | 2021-11-12 | 奥普蒂姆维亚有限公司 | 工程化芳基硫酸酯依赖性酶 |
WO2022015794A1 (en) * | 2020-07-14 | 2022-01-20 | Optimvia, Llc | Methods for synthesizing non-anticoagulant heparan sulfate |
AU2020310172A1 (en) | 2019-07-09 | 2022-03-03 | Optimvia Llc | Methods for synthesizing anticoagulant polysaccharides |
CN114763518B (zh) * | 2021-09-10 | 2023-04-28 | 江南大学 | 发酵生产肝素的酵母工程菌的构建及其应用 |
KR20240172173A (ko) * | 2021-11-05 | 2024-12-09 | 렌슬러 폴리테크닉 인스티튜트 | 저분자량 헤파로산으로부터 저분자량 헤파린을 화학효소적으로 합성하는 방법 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004050673A2 (en) * | 2002-11-27 | 2004-06-17 | Massachusetts Institute Of Technology | Methods for synthesizing polysaccharides |
ITMI20031618A1 (it) * | 2003-08-06 | 2005-02-07 | Inalco Spa | Derivati polisaccaridici dotati di alta attivita' |
WO2005032483A2 (en) | 2003-10-01 | 2005-04-14 | Momenta Pharmaceuticals, Inc. | Polysaccharides for pulmonary delivery of active agents |
GB0419346D0 (en) | 2004-09-01 | 2004-09-29 | Smyth Stephen M F | Method and apparatus for improved headphone virtualisation |
FR2874931B1 (fr) * | 2004-09-08 | 2006-11-24 | Aventis Pharma Sa | Procede de production de polysaccharide k5 |
CN101531723A (zh) | 2009-02-27 | 2009-09-16 | 江南大学 | 一种用生物酶对肝素进行选择性结构修饰制备肝素衍生物的方法 |
WO2012116048A1 (en) * | 2011-02-22 | 2012-08-30 | Rensselaer Polytechnic Institute | Single step heparosan n-deacetylation and depolymerization for making bioengineered heparin |
EP3054005B1 (de) * | 2013-10-02 | 2019-11-20 | Ajinomoto Co., Inc. | Heparosanproduzierendes bakterium und heparosanherstellungsverfahren |
WO2017115674A1 (ja) * | 2015-12-28 | 2017-07-06 | 味の素株式会社 | 抗凝固活性を有するヘパラン硫酸の製造法 |
-
2016
- 2016-12-16 WO PCT/JP2016/087688 patent/WO2017115674A1/ja unknown
- 2016-12-16 HU HUE16881650A patent/HUE053923T2/hu unknown
- 2016-12-16 ES ES16881650T patent/ES2869525T3/es active Active
- 2016-12-16 EP EP16881650.2A patent/EP3399044B1/de active Active
- 2016-12-16 JP JP2017558934A patent/JP6879216B2/ja active Active
-
2018
- 2018-06-26 US US16/018,269 patent/US10704068B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2017115674A1 (ja) | 2017-07-06 |
HUE053923T2 (hu) | 2021-08-30 |
US10704068B2 (en) | 2020-07-07 |
EP3399044B1 (de) | 2021-02-03 |
EP3399044A1 (de) | 2018-11-07 |
EP3399044A4 (de) | 2019-08-07 |
JPWO2017115674A1 (ja) | 2018-10-18 |
US20180298411A1 (en) | 2018-10-18 |
JP6879216B2 (ja) | 2021-06-02 |
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