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ES2869525T3 - Método para producir heparán sulfato que presenta una actividad anticoagulante - Google Patents

Método para producir heparán sulfato que presenta una actividad anticoagulante Download PDF

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Publication number
ES2869525T3
ES2869525T3 ES16881650T ES16881650T ES2869525T3 ES 2869525 T3 ES2869525 T3 ES 2869525T3 ES 16881650 T ES16881650 T ES 16881650T ES 16881650 T ES16881650 T ES 16881650T ES 2869525 T3 ES2869525 T3 ES 2869525T3
Authority
ES
Spain
Prior art keywords
sulfation
heparosan
molecular weight
solution
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES16881650T
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English (en)
Spanish (es)
Inventor
Yuriko Tokura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Application granted granted Critical
Publication of ES2869525T3 publication Critical patent/ES2869525T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726Glycosaminoglycans, i.e. mucopolysaccharides
    • A61K31/727Heparin; Heparan
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/02Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02008Heparin-sulfate lyase (4.2.2.8)

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
ES16881650T 2015-12-28 2016-12-16 Método para producir heparán sulfato que presenta una actividad anticoagulante Active ES2869525T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2015256988 2015-12-28
PCT/JP2016/087688 WO2017115674A1 (ja) 2015-12-28 2016-12-16 抗凝固活性を有するヘパラン硫酸の製造法

Publications (1)

Publication Number Publication Date
ES2869525T3 true ES2869525T3 (es) 2021-10-25

Family

ID=59225768

Family Applications (1)

Application Number Title Priority Date Filing Date
ES16881650T Active ES2869525T3 (es) 2015-12-28 2016-12-16 Método para producir heparán sulfato que presenta una actividad anticoagulante

Country Status (6)

Country Link
US (1) US10704068B2 (de)
EP (1) EP3399044B1 (de)
JP (1) JP6879216B2 (de)
ES (1) ES2869525T3 (de)
HU (1) HUE053923T2 (de)
WO (1) WO2017115674A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017115674A1 (ja) * 2015-12-28 2017-07-06 味の素株式会社 抗凝固活性を有するヘパラン硫酸の製造法
WO2018135027A1 (ja) 2017-01-19 2018-07-26 味の素株式会社 ヘキスロン酸残基が異性化されたヘパロサン化合物の製造方法
EP4050036A1 (de) * 2017-09-05 2022-08-31 Ajinomoto Co., Inc. 3-o-sulfatierungsenzymmutante sowie verfahren zu ihrer verwendung
CN109321508B (zh) * 2018-10-12 2022-12-27 北京化工大学 产heparosan的基因工程菌及其应用
CN113646427A (zh) 2019-01-15 2021-11-12 奥普蒂姆维亚有限公司 工程化芳基硫酸酯依赖性酶
WO2022015794A1 (en) * 2020-07-14 2022-01-20 Optimvia, Llc Methods for synthesizing non-anticoagulant heparan sulfate
AU2020310172A1 (en) 2019-07-09 2022-03-03 Optimvia Llc Methods for synthesizing anticoagulant polysaccharides
CN114763518B (zh) * 2021-09-10 2023-04-28 江南大学 发酵生产肝素的酵母工程菌的构建及其应用
KR20240172173A (ko) * 2021-11-05 2024-12-09 렌슬러 폴리테크닉 인스티튜트 저분자량 헤파로산으로부터 저분자량 헤파린을 화학효소적으로 합성하는 방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004050673A2 (en) * 2002-11-27 2004-06-17 Massachusetts Institute Of Technology Methods for synthesizing polysaccharides
ITMI20031618A1 (it) * 2003-08-06 2005-02-07 Inalco Spa Derivati polisaccaridici dotati di alta attivita'
WO2005032483A2 (en) 2003-10-01 2005-04-14 Momenta Pharmaceuticals, Inc. Polysaccharides for pulmonary delivery of active agents
GB0419346D0 (en) 2004-09-01 2004-09-29 Smyth Stephen M F Method and apparatus for improved headphone virtualisation
FR2874931B1 (fr) * 2004-09-08 2006-11-24 Aventis Pharma Sa Procede de production de polysaccharide k5
CN101531723A (zh) 2009-02-27 2009-09-16 江南大学 一种用生物酶对肝素进行选择性结构修饰制备肝素衍生物的方法
WO2012116048A1 (en) * 2011-02-22 2012-08-30 Rensselaer Polytechnic Institute Single step heparosan n-deacetylation and depolymerization for making bioengineered heparin
EP3054005B1 (de) * 2013-10-02 2019-11-20 Ajinomoto Co., Inc. Heparosanproduzierendes bakterium und heparosanherstellungsverfahren
WO2017115674A1 (ja) * 2015-12-28 2017-07-06 味の素株式会社 抗凝固活性を有するヘパラン硫酸の製造法

Also Published As

Publication number Publication date
WO2017115674A1 (ja) 2017-07-06
HUE053923T2 (hu) 2021-08-30
US10704068B2 (en) 2020-07-07
EP3399044B1 (de) 2021-02-03
EP3399044A1 (de) 2018-11-07
EP3399044A4 (de) 2019-08-07
JPWO2017115674A1 (ja) 2018-10-18
US20180298411A1 (en) 2018-10-18
JP6879216B2 (ja) 2021-06-02

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