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ES2093624T5 - Nuevas proteasas muy alcalinas. - Google Patents

Nuevas proteasas muy alcalinas.

Info

Publication number
ES2093624T5
ES2093624T5 ES90116322T ES90116322T ES2093624T5 ES 2093624 T5 ES2093624 T5 ES 2093624T5 ES 90116322 T ES90116322 T ES 90116322T ES 90116322 T ES90116322 T ES 90116322T ES 2093624 T5 ES2093624 T5 ES 2093624T5
Authority
ES
Spain
Prior art keywords
dna
protests
alkaline
primitive
new
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90116322T
Other languages
English (en)
Other versions
ES2093624T3 (es
Inventor
Roman Vetter
Detlef Wilke
Antoine Amory
Andre Clippe
Wolfgang Aehle
Dietmar Schomburg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Chr Hansen GmbH
Original Assignee
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Genencor International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25884604&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2093624(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Helmholtz Zentrum fuer Infektionsforschung HZI GmbH, Genencor International GmbH filed Critical Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Application granted granted Critical
Publication of ES2093624T3 publication Critical patent/ES2093624T3/es
Publication of ES2093624T5 publication Critical patent/ES2093624T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

EL INVENTO DESCRIBE UNA NUEVA PROTEASA OPTIMIZADA, QUE TIENE ELEVADO CONTENIDO ALCALINO, SE UTILIZA EN FORMULACIONES DE DETERGENCIA Y SE OBTIENE INTERCAMBIANDO LA LISINA O ARGININA DEL AMINOACIDO PRIMITIVO POR MEDIO DE MICROORGANISMOS TRANSFORMADOS A TRAVES DE SECUENCIAS DNA MUTADAS, RESEÑA OLIGONUCLEOTIDOS, VECTORES, SECUENCIAS DNA Y MICROORGANISMOS TRANSFORMADOS QUE SE UTILIZAN PARA LA PRODUCCION Y OBTENCION DE LA PROTESAS OPTIMIZADA CON ELEVADO CONTENIDO ALCALINO Y SE REFIERE A UNA SECUENCIA DNA QUE SE OBTIENE A PARTIR DE SECUENCIAS DNA QUE CODIFICAN LA PROTESAS ALCALINA, SE PRODUCEN A PARTIR DE BACILOS Y SE MODIFICAN EN POSICIONES DETERMINADAS A TRAVES DE MUTAGENESIS ENRIQUECIDAS, YA QUE EL CODON QUE SE ENCUENTRA EN EL PUNTO DE MUTACION CODIFICA EL AMINOACIDO PRIMITIVO Y DA LUGAR AL AMINOACIDO BASICO.
ES90116322T 1989-08-31 1990-08-25 Nuevas proteasas muy alcalinas. Expired - Lifetime ES2093624T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3928804 1989-08-31
DE4023458A DE4023458A1 (de) 1989-08-31 1990-07-24 Neue hochalkalische proteasen

Publications (2)

Publication Number Publication Date
ES2093624T3 ES2093624T3 (es) 1997-01-01
ES2093624T5 true ES2093624T5 (es) 2001-08-16

Family

ID=25884604

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90116322T Expired - Lifetime ES2093624T5 (es) 1989-08-31 1990-08-25 Nuevas proteasas muy alcalinas.

Country Status (7)

Country Link
EP (1) EP0415296B2 (es)
JP (1) JPH03139277A (es)
KR (1) KR100196785B1 (es)
AT (1) ATE143689T1 (es)
DE (2) DE4023458A1 (es)
DK (1) DK0415296T4 (es)
ES (1) ES2093624T5 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0769549A3 (en) * 1990-12-21 1997-05-21 Novo Nordisk A/S Enzyme mutants having a low degree of variation of the molecular charge over a pH range
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DE4106525A1 (de) * 1991-03-01 1992-09-10 Solvay Enzymes Gmbh & Co Kg Verfahren zur verbesserung der stabilitaet von hochalkalischen proteasen
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
DE4224125A1 (de) * 1991-07-27 1993-01-28 Solvay Enzymes Gmbh & Co Kg Verfahren zur verbesserung der stabilitaet von enzymen und stabilisierte enzyme
DE69223562T2 (de) * 1991-08-27 1998-06-04 Hoffmann La Roche Verfahren und Reagenzien zum Nachweis von Hepatitis C
US5371198A (en) * 1991-12-16 1994-12-06 Novo Nordisk A/S Method for protection of proteolysis-susceptible protein during protein production in a fluid medium
US5256557A (en) * 1991-12-27 1993-10-26 Solvay Enzymes, Inc. Purified alkaline protease concentrate and method of preparation
US5623059A (en) * 1992-03-09 1997-04-22 Novo Nordisk A/S Method for protection of proteolysis-susceptible protein during protein production in a fluid medium
DE69333463D1 (de) * 1992-05-18 2004-05-06 Genencor Int Alkalische Proteasen produzierende Bakterien, und Verfahren zur Herstellung dieser alkalischen Proteasen
ATE376061T1 (de) * 1992-07-17 2007-11-15 Genencor Int Hochalkalische serinproteasen
DE4304161A1 (de) * 1993-02-12 1994-08-25 Solvay Enzymes Gmbh & Co Kg Verbesserte hochalkalische Proteasen
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
EP2258837A1 (en) 2004-09-10 2010-12-08 Novozymes North America, Inc. Methods for preventing, removing, reducing, or disrupting biofilm
JP5813753B2 (ja) * 2010-05-06 2015-11-17 ザ プロクター アンド ギャンブルカンパニー プロテアーゼ変異体を有する消費者製品
CN103080415B (zh) 2010-07-01 2016-08-10 诺维信公司 纸浆的漂白
MX338925B (es) 2011-05-05 2016-05-06 Procter & Gamble Composiciones y metodos que comprenden variantes de proteasa serina.
CN103764905A (zh) 2011-09-09 2014-04-30 诺维信公司 改进纸张材料的特性
EP2904101B1 (en) * 2012-10-03 2019-06-12 Agrivida, Inc. Intein-modified proteases, their production and industrial applications
EP2740840A1 (en) 2012-12-07 2014-06-11 Novozymes A/S Improving drainage of paper pulp
WO2016007309A1 (en) 2014-07-07 2016-01-14 Novozymes A/S Use of prehydrolysate liquor in engineered wood
WO2021018751A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Enzymatic treatment of paper pulp
EP4158097A1 (en) 2020-05-29 2023-04-05 Novozymes A/S Method for controlling slime in a pulp or paper making process
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2022263553A1 (en) 2021-06-16 2022-12-22 Novozymes A/S Method for controlling slime in a pulp or paper making process

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3770587A (en) * 1971-02-09 1973-11-06 Pfizer Chemically modified proteolytic enzymes
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
FR2598430B1 (fr) * 1986-05-06 1990-02-02 Roussel Uclaf Nouveaux vecteurs d'expression, leur procede d'obtention et leur application pour exprimer une proteine quelconque dans escherichia coli
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
DK316989D0 (da) * 1989-06-26 1989-06-26 Novo Nordisk As Enzymer

Also Published As

Publication number Publication date
DE59010522D1 (de) 1996-11-07
DK0415296T3 (da) 1996-11-18
DE4023458A1 (de) 1991-03-07
EP0415296B1 (de) 1996-10-02
KR910004806A (ko) 1991-03-29
JPH03139277A (ja) 1991-06-13
EP0415296B2 (de) 2001-04-25
EP0415296A2 (de) 1991-03-06
EP0415296A3 (en) 1991-10-02
ES2093624T3 (es) 1997-01-01
ATE143689T1 (de) 1996-10-15
KR100196785B1 (ko) 1999-06-15
DK0415296T4 (da) 2001-11-12

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