[go: up one dir, main page]

EP1287154A4 - Ladungswechsel-nukleotide - Google Patents

Ladungswechsel-nukleotide

Info

Publication number
EP1287154A4
EP1287154A4 EP01946213A EP01946213A EP1287154A4 EP 1287154 A4 EP1287154 A4 EP 1287154A4 EP 01946213 A EP01946213 A EP 01946213A EP 01946213 A EP01946213 A EP 01946213A EP 1287154 A4 EP1287154 A4 EP 1287154A4
Authority
EP
European Patent Office
Prior art keywords
charge
switch nucleotides
nucleotides
switch
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP01946213A
Other languages
English (en)
French (fr)
Other versions
EP1287154A1 (de
Inventor
John G K Williams
Gregory R Bashford
Jiyan Chen
Dan Draney
Nara Narayanan
Bambi L Reynolds
Pamela Sheaff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pacific Biosciences of California Inc
Original Assignee
Pacific Biosciences of California Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/876,375 external-priority patent/US6869764B2/en
Priority claimed from US09/876,374 external-priority patent/US6936702B2/en
Application filed by Pacific Biosciences of California Inc filed Critical Pacific Biosciences of California Inc
Priority to EP12172239.1A priority Critical patent/EP2634263B1/de
Priority to EP09008795.8A priority patent/EP2226330B1/de
Priority to EP16180833.2A priority patent/EP3112478B1/de
Publication of EP1287154A1 publication Critical patent/EP1287154A1/de
Publication of EP1287154A4 publication Critical patent/EP1287154A4/de
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP01946213A 2000-06-07 2001-06-07 Ladungswechsel-nukleotide Ceased EP1287154A4 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP12172239.1A EP2634263B1 (de) 2000-06-07 2001-06-07 Ladeschaltungs-Nukleotidebasiertes Verfahren zur Sequenzierung von Nukleinsäuren
EP09008795.8A EP2226330B1 (de) 2000-06-07 2001-06-07 Ladeschaltungs-Nukleotide
EP16180833.2A EP3112478B1 (de) 2000-06-07 2001-06-07 Auf ladeschaltungsnukleotiden basierendes verfahren zur sequenzierung von nukleinsäuren

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US876374 1997-06-16
US20989600P 2000-06-07 2000-06-07
US209896P 2000-06-07
US28623801P 2001-04-24 2001-04-24
US286238P 2001-04-24
US876375 2001-06-06
US09/876,375 US6869764B2 (en) 2000-06-07 2001-06-06 Nucleic acid sequencing using charge-switch nucleotides
US09/876,374 US6936702B2 (en) 2000-06-07 2001-06-06 Charge-switch nucleotides
PCT/US2001/018699 WO2001094609A1 (en) 2000-06-07 2001-06-07 Charge-switch nucleotides

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP16180833.2A Division EP3112478B1 (de) 2000-06-07 2001-06-07 Auf ladeschaltungsnukleotiden basierendes verfahren zur sequenzierung von nukleinsäuren
EP12172239.1A Division EP2634263B1 (de) 2000-06-07 2001-06-07 Ladeschaltungs-Nukleotidebasiertes Verfahren zur Sequenzierung von Nukleinsäuren

Publications (2)

Publication Number Publication Date
EP1287154A1 EP1287154A1 (de) 2003-03-05
EP1287154A4 true EP1287154A4 (de) 2010-12-15

Family

ID=27498783

Family Applications (2)

Application Number Title Priority Date Filing Date
EP09008795.8A Expired - Lifetime EP2226330B1 (de) 2000-06-07 2001-06-07 Ladeschaltungs-Nukleotide
EP01946213A Ceased EP1287154A4 (de) 2000-06-07 2001-06-07 Ladungswechsel-nukleotide

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP09008795.8A Expired - Lifetime EP2226330B1 (de) 2000-06-07 2001-06-07 Ladeschaltungs-Nukleotide

Country Status (5)

Country Link
EP (2) EP2226330B1 (de)
JP (1) JP2004516810A (de)
AU (1) AU2001268291A1 (de)
CA (1) CA2412567A1 (de)
WO (1) WO2001094609A1 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
EP1354064A2 (de) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
US7560254B2 (en) 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
GB0128526D0 (en) * 2001-11-29 2002-01-23 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides
DE10224339A1 (de) * 2002-05-29 2003-12-11 Axaron Bioscience Ag Verfahren zur hochparallelen Nukleinsäuresequenzierung
ATE466956T1 (de) * 2002-08-29 2010-05-15 Ge Healthcare Bio Sciences Analytnachweis
CA2496462C (en) * 2002-08-29 2014-01-28 Amersham Biosciences Corp Allele specific primer extension
AU2003263023B2 (en) * 2002-08-29 2008-09-11 Global Life Sciences Solutions Usa Llc Terminal phosphate blocked nucleoside polyphosphates
EP1590479B1 (de) * 2003-02-05 2010-11-24 GE Healthcare Bio-Sciences Corp. Nukleinsäureamplifikation
CA2513690A1 (en) * 2003-02-05 2004-08-26 Amersham Biosciences Corp Solid phase sequencing
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 核酸シーケンシング用組成物及び方法
CN107083421A (zh) 2010-12-17 2017-08-22 纽约哥伦比亚大学理事会 使用经修饰的核苷酸和纳米孔检测的dna边合成边测序
CN102621214B (zh) * 2012-03-13 2014-10-29 美国哈佛大学 一种基于固态纳米孔对核酸分子进行减速及单分子捕获的方法
WO2013154999A2 (en) 2012-04-09 2013-10-17 The Trustees Of Columbia University In The City Of New York Method of preparation of nanopore and uses thereof
US20150119259A1 (en) * 2012-06-20 2015-04-30 Jingyue Ju Nucleic acid sequencing by nanopore detection of tag molecules
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
WO2014144898A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
KR102221637B1 (ko) 2013-11-17 2021-03-02 퀀텀-에스아이 인코포레이티드 분자들을 검사, 검출 및 분석하기 위한 광 시스템 및 어세이 칩
AU2015236392A1 (en) * 2014-03-24 2016-10-20 Genia Technologies, Inc. Chemical methods for producing tagged nucleotides
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
CA2957546A1 (en) 2014-08-08 2016-02-11 Quantum-Si Incorporated Integrated device with external light source for probing, detecting, and analyzing molecules
KR102384909B1 (ko) 2014-08-08 2022-04-11 퀀텀-에스아이 인코포레이티드 수신된 광자들의 시간 비닝을 위한 집적 디바이스
JP6812341B2 (ja) 2014-08-08 2021-01-13 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ 分子の探索、検出及び解析のための光学システム及びアッセイチップ
EP3315461B1 (de) * 2015-06-23 2021-07-07 BGI Shenzhen Mikroporöse elektrode und verfahren zur analyse chemischer substanzen
KR102626317B1 (ko) 2017-07-24 2024-01-18 퀀텀-에스아이 인코포레이티드 고강도 표지된 반응물 조성물 및 서열분석 방법
JP6987898B2 (ja) * 2018-02-16 2022-01-05 イラミーナ インコーポレーテッド 標識ヌクレオチドおよびその使用
CA3127102A1 (en) 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
JP2022521730A (ja) 2019-02-19 2022-04-12 ウルティマ・ゲノミクス・インコーポレーテッド 光検出および配列決定のためのリンカーおよび方法
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
CN115028547B (zh) * 2022-05-17 2023-09-12 浙江华伟医药科技有限公司 一种邻氨基苯甲酰胺的微通道化学合成方法
CN116375780B (zh) * 2023-06-02 2023-08-15 山东大学 一种用于识别gtp结合蛋白的光亲和探针及其制备方法和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5260433A (en) * 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1997000967A1 (en) * 1995-06-23 1997-01-09 Baylor College Of Medicine Alternative dye-labeled primers, ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated dna analysis and homogeneous amplification/detection assays
EP0967219A1 (de) * 1997-07-11 1999-12-29 The Institute Of Physical & Chemical Research Verbindungen mit energieübertragungsfunktion und eine methode zur dna-basensequenzierung unter verwendung dieser verbindungen

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3687808A (en) 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4973679A (en) 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US4739044A (en) 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4855225A (en) 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
FR2596761B1 (fr) 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US4793705A (en) 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
US4979824A (en) 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US6984487B1 (en) 1989-08-22 2006-01-10 Hsc Research Development Corporation Cystic fibrosis gene
US5578443A (en) 1991-03-06 1996-11-26 Regents Of The University Of Minnesota DNA sequence-based HLA typing method
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5681940A (en) 1994-11-02 1997-10-28 Icn Pharmaceuticals Sugar modified nucleosides and oligonucleotides
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5814524A (en) 1995-12-14 1998-09-29 Trustees Of Tufts College Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations
GB9626815D0 (en) * 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
CA2355816C (en) * 1998-12-14 2007-10-30 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6323186B1 (en) * 1999-09-17 2001-11-27 Molecular Probes, Inc. Phosphate-bound polyazaindacene derivatives of nucleotides

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5260433A (en) * 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1997000967A1 (en) * 1995-06-23 1997-01-09 Baylor College Of Medicine Alternative dye-labeled primers, ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated dna analysis and homogeneous amplification/detection assays
EP0967219A1 (de) * 1997-07-11 1999-12-29 The Institute Of Physical & Chemical Research Verbindungen mit energieübertragungsfunktion und eine methode zur dna-basensequenzierung unter verwendung dieser verbindungen

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GOODWIN P M ET AL: "APPLICATION OF SINGLE MOLECULE DETECTION TO DNA SEQUENCING", NUCLEOSIDES & NUCLEOTIDES, MARCEL DEKKER, INC, US, vol. 16, no. 5/06, 1 January 1997 (1997-01-01), pages 543 - 550, XP001109270, ISSN: 0732-8311 *
K. C. TSOU ET AL.: "Indigogenic Phosphodiesters as Potential Chromogenic Cancer Chemotherapeutic Agents", JOURNAL OF MEDICIANL CHEMISTRY, vol. 25, no. 12, 1972, pages 1221 - 1225, XP002591210 *
RONAGHI ET AL: "A SEQUENCING METHOD BASED ON REAL-TIME PYROPHOSPHATE", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, WASHINGTON, DC; US LNKD- DOI:10.1126/SCIENCE.281.5375.363, vol. 281, 17 July 1998 (1998-07-17), pages 363 - 365, XP002135869, ISSN: 0036-8075 *
See also references of WO0194609A1 *

Also Published As

Publication number Publication date
JP2004516810A (ja) 2004-06-10
CA2412567A1 (en) 2001-12-13
AU2001268291A1 (en) 2001-12-17
EP1287154A1 (de) 2003-03-05
EP2226330B1 (de) 2013-09-18
WO2001094609A1 (en) 2001-12-13
EP2226330A1 (de) 2010-09-08

Similar Documents

Publication Publication Date Title
EP1287154A4 (de) Ladungswechsel-nukleotide
DE60105852D1 (en) Fluidmischsystem
DE50112082D1 (en) Bulk-acoustic-wave-filter
AU3391801A (en) Pyridinylimidazoles
AU8274301A (en) Electro-powder
DE50105205D1 (en) Creatinin-biosensor
AU6803901A (en) Bis-arylsulfones
DE50112740D1 (en) Strangführungselement
DE50110569D1 (en) Substituierte c-cyclohexylmethylamin-derivate
DE50112014D1 (en) Stiftheizer
DE50107703D1 (en) Creatininsensor-kalibration
AU9385201A (en) Sulfonylguanidine
AU6619001A (en) Liquid-pourers
DE50114303D1 (en) Nsaugluftkanal
DE50008447D1 (en) Aminomethyl-phenyl-cyclohexanonderivate
AU6424701A (en) Gasc1 gene
DE50114375D1 (en) Compound-reibungsvakuumpumpe
AU4686401A (en) Hypotensors
AU9187601A (en) Triazolo-epothilones
DE50006446D1 (en) Otoplastik
GB0028849D0 (en) Improved keysheath
DE60107852D1 (en) Thienopyrrolidinone
DE50003404D1 (en) Multi-master-bus-system
GB0004254D0 (en) Improved capodastro
GB0125945D0 (en) Putter-heads

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20021212

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO SI

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: LI-COR, INC.

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.

A4 Supplementary search report drawn up and despatched

Effective date: 20101111

RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/68 20060101ALI20101105BHEP

Ipc: C07H 19/20 20060101ALI20101105BHEP

Ipc: C07H 19/10 20060101AFI20101105BHEP

17Q First examination report despatched

Effective date: 20110404

REG Reference to a national code

Ref country code: DE

Ref legal event code: R003

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED

18R Application refused

Effective date: 20120619