AU2001268291A1 - Charge-switch nucleotides - Google Patents

Charge-switch nucleotides

Info

Publication number: AU2001268291A1
Authority: AU; Australia
Prior art keywords: charge; probe; phosphate; switch; intact
Prior art date: 2000-06-07
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

AU2001268291A

Other languages

English (en)

Inventor

Gregory R. Bashford

Jiyan Chen

Dan Draney

Nara Narayanan

Bambi L. Reynolds

Pamela Sheaff

John G.K. Williams

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Li Cor Inc

Original Assignee

Li Cor Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-06-07

Filing date

2001-06-07

Publication date

2001-12-17

2001-06-06 Priority claimed from US09/876,374 external-priority patent/US6936702B2/en

2001-06-06 Priority claimed from US09/876,375 external-priority patent/US6869764B2/en

2001-06-07 Application filed by Li Cor Inc filed Critical Li Cor Inc

2001-12-17 Publication of AU2001268291A1 publication Critical patent/AU2001268291A1/en

Status Abandoned legal-status Critical Current

Links

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125000003729 nucleotide group Chemical group 0.000 title claims description 124
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239000010452 phosphate Substances 0.000 claims description 151
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125000005647 linker group Chemical group 0.000 claims description 42
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UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims description 28
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DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
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XRECTZIEBJDKEO-UHFFFAOYSA-N flucytosine Chemical compound NC1=NC(=O)NC=C1F XRECTZIEBJDKEO-UHFFFAOYSA-N 0.000 description 1
229960004413 flucytosine Drugs 0.000 description 1
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CPBQJMYROZQQJC-UHFFFAOYSA-N helium neon Chemical compound [He].[Ne] CPBQJMYROZQQJC-UHFFFAOYSA-N 0.000 description 1
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235000018977 lysine Nutrition 0.000 description 1
150000002669 lysines Chemical class 0.000 description 1
UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
229940096405 magnesium cation Drugs 0.000 description 1
WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
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150000004706 metal oxides Chemical class 0.000 description 1
125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 description 1
125000000325 methylidene group Chemical group [H]C([H])=* 0.000 description 1
244000005700 microbiome Species 0.000 description 1
239000004005 microsphere Substances 0.000 description 1
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238000010369 molecular cloning Methods 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
150000004712 monophosphates Chemical class 0.000 description 1
ZUHZZVMEUAUWHY-UHFFFAOYSA-N n,n-dimethylpropan-1-amine Chemical compound CCCN(C)C ZUHZZVMEUAUWHY-UHFFFAOYSA-N 0.000 description 1
FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
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150000008300 phosphoramidites Chemical class 0.000 description 1
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239000013612 plasmid Substances 0.000 description 1
229910052697 platinum Inorganic materials 0.000 description 1
229920000058 polyacrylate Polymers 0.000 description 1
229920002647 polyamide Polymers 0.000 description 1
229920000573 polyethylene Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
229920006254 polymer film Polymers 0.000 description 1
238000003752 polymerase chain reaction Methods 0.000 description 1
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239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
239000011591 potassium Substances 0.000 description 1
229910052700 potassium Inorganic materials 0.000 description 1
230000008569 process Effects 0.000 description 1
239000012264 purified product Substances 0.000 description 1
IGFXRKMLLMBKSA-UHFFFAOYSA-N purine Chemical compound N1=C[N]C2=NC=NC2=C1 IGFXRKMLLMBKSA-UHFFFAOYSA-N 0.000 description 1
239000002213 purine nucleotide Substances 0.000 description 1
150000003212 purines Chemical group 0.000 description 1
239000002718 pyrimidine nucleoside Substances 0.000 description 1
239000002719 pyrimidine nucleotide Substances 0.000 description 1
150000003230 pyrimidines Chemical group 0.000 description 1
239000010453 quartz Substances 0.000 description 1
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239000012146 running buffer Substances 0.000 description 1
239000012488 sample solution Substances 0.000 description 1
238000000926 separation method Methods 0.000 description 1
FZHAPNGMFPVSLP-UHFFFAOYSA-N silanamine Chemical compound [SiH3]N FZHAPNGMFPVSLP-UHFFFAOYSA-N 0.000 description 1
229910052710 silicon Inorganic materials 0.000 description 1
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238000004088 simulation Methods 0.000 description 1
239000011734 sodium Substances 0.000 description 1
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239000011780 sodium chloride Substances 0.000 description 1
ZNJHFNUEQDVFCJ-UHFFFAOYSA-M sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid;hydroxide Chemical compound [OH-].[Na+].OCCN1CCN(CCS(O)(=O)=O)CC1 ZNJHFNUEQDVFCJ-UHFFFAOYSA-M 0.000 description 1
238000004611 spectroscopical analysis Methods 0.000 description 1
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125000001424 substituent group Chemical group 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
229940014800 succinic anhydride Drugs 0.000 description 1
229960002317 succinimide Drugs 0.000 description 1
125000000542 sulfonic acid group Chemical group 0.000 description 1
125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 description 1
239000011593 sulfur Substances 0.000 description 1
125000004434 sulfur atom Chemical group 0.000 description 1
229920005613 synthetic organic polymer Polymers 0.000 description 1
230000002123 temporal effect Effects 0.000 description 1
150000007970 thio esters Chemical class 0.000 description 1
150000003568 thioethers Chemical class 0.000 description 1
RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
229960004072 thrombin Drugs 0.000 description 1
229940104230 thymidine Drugs 0.000 description 1
229940086542 triethylamine Drugs 0.000 description 1
SRPWOOOHEPICQU-UHFFFAOYSA-N trimellitic anhydride Chemical compound OC(=O)C1=CC=C2C(=O)OC(=O)C2=C1 SRPWOOOHEPICQU-UHFFFAOYSA-N 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
230000005751 tumor progression Effects 0.000 description 1
238000005406 washing Methods 0.000 description 1
239000001018 xanthene dye Substances 0.000 description 1
150000003732 xanthenes Chemical class 0.000 description 1
229910052725 zinc Inorganic materials 0.000 description 1
239000011701 zinc Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Investigating Or Analysing Biological Materials (AREA)

AU2001268291A 2000-06-07 2001-06-07 Charge-switch nucleotides Abandoned AU2001268291A1 (en)

Applications Claiming Priority (9)

Application Number	Priority Date	Filing Date	Title
US20989600P	2000-06-07	2000-06-07
US60209896		2000-06-07
US28623801P	2001-04-24	2001-04-24
US60286238		2001-04-24
US09/876,374 US6936702B2 (en)	2000-06-07	2001-06-06	Charge-switch nucleotides
US09/876,375 US6869764B2 (en)	2000-06-07	2001-06-06	Nucleic acid sequencing using charge-switch nucleotides
US09876374		2001-06-06
US09876375		2001-06-06
PCT/US2001/018699 WO2001094609A1 (en)	2000-06-07	2001-06-07	Charge-switch nucleotides

Publications (1)

Publication Number	Publication Date
AU2001268291A1 true AU2001268291A1 (en)	2001-12-17

Family

ID=27498783

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AU2001268291A Abandoned AU2001268291A1 (en)	2000-06-07	2001-06-07	Charge-switch nucleotides

Country Status (5)

Country	Link
EP (2)	EP1287154A4 (de)
JP (1)	JP2004516810A (de)
AU (1)	AU2001268291A1 (de)
CA (1)	CA2412567A1 (de)
WO (1)	WO2001094609A1 (de)

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2001
- 2001-06-07 CA CA002412567A patent/CA2412567A1/en not_active Abandoned
- 2001-06-07 JP JP2002502149A patent/JP2004516810A/ja active Pending
- 2001-06-07 EP EP01946213A patent/EP1287154A4/de not_active Ceased
- 2001-06-07 AU AU2001268291A patent/AU2001268291A1/en not_active Abandoned
- 2001-06-07 EP EP09008795.8A patent/EP2226330B1/de not_active Expired - Lifetime
- 2001-06-07 WO PCT/US2001/018699 patent/WO2001094609A1/en active Application Filing

Also Published As

Publication number	Publication date
EP1287154A4 (de)	2010-12-15
CA2412567A1 (en)	2001-12-13
EP1287154A1 (de)	2003-03-05
EP2226330A1 (de)	2010-09-08
JP2004516810A (ja)	2004-06-10
EP2226330B1 (de)	2013-09-18
WO2001094609A1 (en)	2001-12-13

Publication	Publication Date	Title
EP3112478B1 (de)	2017-12-06	Auf ladeschaltungsnukleotiden basierendes verfahren zur sequenzierung von nukleinsäuren
US6869764B2 (en)	2005-03-22	Nucleic acid sequencing using charge-switch nucleotides
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