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EA200101078A1 - Получение биологически активных молекул - Google Patents

Получение биологически активных молекул

Info

Publication number
EA200101078A1
EA200101078A1 EA200101078A EA200101078A EA200101078A1 EA 200101078 A1 EA200101078 A1 EA 200101078A1 EA 200101078 A EA200101078 A EA 200101078A EA 200101078 A EA200101078 A EA 200101078A EA 200101078 A1 EA200101078 A1 EA 200101078A1
Authority
EA
Eurasian Patent Office
Prior art keywords
active molecules
biological active
obtaining biological
biologically active
cleavage
Prior art date
Application number
EA200101078A
Other languages
English (en)
Other versions
EA006825B1 (ru
Inventor
Менахем Рубинштейн
Бианлинг Лиу
Даниэла Новик
Чарльз Динарелло
Пьер Грабэр
Original Assignee
Йеда Рисерч Энд Дивелопмент Ко.Лтд.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Йеда Рисерч Энд Дивелопмент Ко.Лтд. filed Critical Йеда Рисерч Энд Дивелопмент Ко.Лтд.
Publication of EA200101078A1 publication Critical patent/EA200101078A1/ru
Publication of EA006825B1 publication Critical patent/EA006825B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Steroid Compounds (AREA)

Abstract

Биологически активные молекулы получают из их биологически неактивных предшественников мутацией их нативных участков расщепления с образованием участка, расщепляемого протеазой, и расщеплением мутированной молекулы с получением биологически активной молекулы.Отчет о международном поиске был опубликован 2001.02.08.
EA200101078A 1999-04-13 2000-04-13 Способы получения биологически активных молекул EA006825B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL12942799A IL129427A0 (en) 1999-04-13 1999-04-13 Preparation of biologically active molecules
PCT/IL2000/000220 WO2000061768A2 (en) 1999-04-13 2000-04-13 Preparation of biologically active molecules

Publications (2)

Publication Number Publication Date
EA200101078A1 true EA200101078A1 (ru) 2002-04-25
EA006825B1 EA006825B1 (ru) 2006-04-28

Family

ID=11072700

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200101078A EA006825B1 (ru) 1999-04-13 2000-04-13 Способы получения биологически активных молекул

Country Status (23)

Country Link
US (1) US7098002B1 (ru)
EP (1) EP1169460B1 (ru)
JP (1) JP4822587B2 (ru)
KR (1) KR100682185B1 (ru)
CN (1) CN1324140C (ru)
AR (1) AR023468A1 (ru)
AT (1) ATE326536T1 (ru)
AU (1) AU781342B2 (ru)
BR (1) BR0009782A (ru)
CA (1) CA2368994C (ru)
DE (1) DE60028022T2 (ru)
DK (1) DK1169460T3 (ru)
EA (1) EA006825B1 (ru)
EE (1) EE05591B1 (ru)
ES (1) ES2259287T3 (ru)
IL (3) IL129427A0 (ru)
MX (1) MXPA01010487A (ru)
NO (1) NO329525B1 (ru)
PT (1) PT1169460E (ru)
SI (1) SI1169460T1 (ru)
UA (1) UA73940C2 (ru)
WO (1) WO2000061768A2 (ru)
ZA (1) ZA200108364B (ru)

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US7273722B2 (en) * 2000-11-29 2007-09-25 Allergan, Inc. Neurotoxins with enhanced target specificity
KR100862136B1 (ko) 2001-03-08 2008-10-09 아레스 트레이딩 에스.아. 인터루킨-18 변이체, 이들의 생산 및 용도
IL142246A0 (en) * 2001-03-26 2002-03-10 Applied Research Systems Method and kit for quantitation of polypeptides
EP1483298A4 (en) 2002-02-11 2007-03-14 Zymogenetics Inc MATERIALS AND METHODS FOR PREPARING DIMERED GROWTH FACTORS
US20060099689A1 (en) * 2003-02-21 2006-05-11 Mertens Nico M A Use of caspase enzymes for maturation of engineered recombinant polypeptide fusions
US7824885B2 (en) * 2003-08-06 2010-11-02 University Of Maryland, College Park Engineered proteases for affinity purification and processing of fusion proteins
EP1689877A1 (en) * 2003-11-24 2006-08-16 Novo Nordisk A/S Fusion proteins and methods of cleavage of such proteins
CA2558811A1 (en) 2004-03-08 2005-09-22 Zymogenetics, Inc. Dimeric fusion proteins and materials and methods for producing them
AU2006339490B2 (en) 2005-09-19 2011-12-08 Allergan, Inc. Clostridial toxin activatable clostridial toxins
ATE548380T1 (de) * 2006-04-20 2012-03-15 Kringle Pharma Inc Mutante des hgf-precursor-proteins und aktivierte form davon
DE102007008596B4 (de) * 2007-02-15 2010-09-02 Friedrich-Schiller-Universität Jena Biologisch wirksame Moleküle auf Grundlage von PNA und siRNA, Verfahren zu deren zellspezifischen Aktivierung sowie Applikationskit zur Verabreichung
CA3128656A1 (en) 2007-08-22 2009-02-26 The Regents Of The University Of California Activatable binding polypeptides and methods of identification and use thereof
KR100980315B1 (ko) * 2007-10-01 2010-09-07 한국생명공학연구원 대장균시스템을 이용한 인위적 활성화가 가능한 카스파제전구체 대량생산 및 활성화 방법
DE102009043743B4 (de) 2009-03-13 2016-10-13 Friedrich-Schiller-Universität Jena Zellspezifisch wirksame Moleküle auf Grundlage von siRNA sowie Applikationskits zu deren Herstellung und Verwendung
CN102482347B (zh) 2009-01-12 2017-04-26 希托马克斯医疗有限责任公司 修饰抗体组合物及其制备和使用方法
CN102481341B (zh) 2009-02-23 2017-05-17 希托马克斯医疗有限公司 蛋白原及其使用方法
CN103923897B (zh) * 2013-01-11 2016-06-01 中国人民解放军军事医学科学院生物工程研究所 caspase重组酶原及其编码基因与应用
CN103388001B (zh) * 2013-07-16 2016-01-20 中国人民解放军第四军医大学 一种含凝血酶切割位点gst膜型表达载体及转染阳性细胞的方法
RU2711322C1 (ru) 2013-12-20 2020-01-16 Ф. Хоффманн-Ля Рош Аг Улучшенные способы получения рекомбинантного полипептида
SG11201707370WA (en) 2015-03-30 2017-10-30 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
EP4074730A1 (en) 2015-06-24 2022-10-19 F. Hoffmann-La Roche AG Anti-transferrin receptor antibodies with tailored affinity
CR20180149A (es) 2015-10-02 2018-04-05 Hoffmann La Roche Anticuerpos biespecíficos contra el cd20 humano y el receptor de transferrina humano y métodos de uso
AR106189A1 (es) 2015-10-02 2017-12-20 Hoffmann La Roche ANTICUERPOS BIESPECÍFICOS CONTRA EL A-b HUMANO Y EL RECEPTOR DE TRANSFERRINA HUMANO Y MÉTODOS DE USO
CN118667900A (zh) 2016-04-06 2024-09-20 绿光生物科技股份有限公司 无细胞生成核糖核酸
SG11202003192UA (en) 2017-10-11 2020-05-28 Greenlight Biosciences Inc Methods and compositions for nucleoside triphosphate and ribonucleic acid production
KR20200080749A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 N-말단의 메티오닌이 절단된 목적 단백질 발현용 유전자 발현 카세트 및 이를 이용하여 n-말단의 메티오닌이 절단된 목적 단백질을 생산하는 방법
EP4013857A1 (en) * 2019-08-13 2022-06-22 King's College London Immunoresponsive cells armoured with spatiotemporally restricted activity of cytokines of the il-1 superfamily

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DE69229252T2 (de) * 1991-08-16 1999-12-16 Merck & Co., Inc. DNS, welche das Interleukin-1B-Vorläufer-Converting-Enzym kodiert
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Also Published As

Publication number Publication date
AR023468A1 (es) 2002-09-04
ES2259287T3 (es) 2006-10-01
WO2000061768A3 (en) 2001-02-08
EA006825B1 (ru) 2006-04-28
CN1353761A (zh) 2002-06-12
DE60028022T2 (de) 2006-09-28
US7098002B1 (en) 2006-08-29
KR100682185B1 (ko) 2007-02-12
CN1324140C (zh) 2007-07-04
EE200100530A (et) 2002-12-16
EE05591B1 (et) 2012-10-15
KR20020007365A (ko) 2002-01-26
PT1169460E (pt) 2006-07-31
MXPA01010487A (es) 2002-05-06
CA2368994A1 (en) 2000-10-19
IL145842A (en) 2008-06-05
IL145842A0 (en) 2002-07-25
UA73940C2 (ru) 2005-10-17
DK1169460T3 (da) 2006-09-18
NO20014966D0 (no) 2001-10-12
EP1169460A2 (en) 2002-01-09
JP4822587B2 (ja) 2011-11-24
JP2002541813A (ja) 2002-12-10
HK1044351A1 (en) 2002-10-18
AU3985100A (en) 2000-11-14
ZA200108364B (en) 2002-10-11
NO20014966L (no) 2001-12-11
DE60028022D1 (de) 2006-06-22
CA2368994C (en) 2010-03-09
NO329525B1 (no) 2010-11-01
SI1169460T1 (sl) 2006-08-31
IL129427A0 (en) 2000-02-17
BR0009782A (pt) 2002-01-08
AU781342B2 (en) 2005-05-19
WO2000061768A2 (en) 2000-10-19
ATE326536T1 (de) 2006-06-15
EP1169460B1 (en) 2006-05-17

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU