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DK3684930T3 - Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression - Google Patents

Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression Download PDF

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Publication number
DK3684930T3
DK3684930T3 DK18786034.1T DK18786034T DK3684930T3 DK 3684930 T3 DK3684930 T3 DK 3684930T3 DK 18786034 T DK18786034 T DK 18786034T DK 3684930 T3 DK3684930 T3 DK 3684930T3
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Denmark
Prior art keywords
inactivation
specificity
modification
gene expression
rna molecules
Prior art date
Application number
DK18786034.1T
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English (en)
Inventor
Eyal Maori
Yaron Galanty
Cristina Pignocchi
Garcia Angela Chaparro
Ofir Meir
Original Assignee
Tropic Biosciences Uk Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1715116.8A external-priority patent/GB201715116D0/en
Priority claimed from GBGB1715113.5A external-priority patent/GB201715113D0/en
Priority claimed from GBGB1719516.5A external-priority patent/GB201719516D0/en
Application filed by Tropic Biosciences Uk Ltd filed Critical Tropic Biosciences Uk Ltd
Application granted granted Critical
Publication of DK3684930T3 publication Critical patent/DK3684930T3/da

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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  • Health & Medical Sciences (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Environmental Sciences (AREA)
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  • Developmental Biology & Embryology (AREA)
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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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DK18786034.1T 2017-09-19 2018-09-18 Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression DK3684930T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB1715116.8A GB201715116D0 (en) 2017-09-19 2017-09-19 Modifying the specificity of non-coding RNA molecules for silencing of target RNA in eukaryotic cells
GBGB1715113.5A GB201715113D0 (en) 2017-09-19 2017-09-19 Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA
GBGB1719516.5A GB201719516D0 (en) 2017-11-23 2017-11-23 Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA
PCT/IB2018/057160 WO2019058255A1 (en) 2017-09-19 2018-09-18 MODIFICATION OF THE SPECIFICITY OF VEGETABLE NON-CODANT RNA MOLECULES FOR THE SILENCING OF GENE EXPRESSION

Publications (1)

Publication Number Publication Date
DK3684930T3 true DK3684930T3 (da) 2022-06-07

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DK18786034.1T DK3684930T3 (da) 2017-09-19 2018-09-18 Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression

Country Status (13)

Country Link
US (5) US11555199B2 (da)
EP (3) EP3684929A1 (da)
JP (3) JP7422069B2 (da)
KR (4) KR20250161608A (da)
CN (4) CN111373044B (da)
AU (4) AU2018336128B2 (da)
BR (2) BR112020005416A2 (da)
CA (2) CA3074948A1 (da)
DK (1) DK3684930T3 (da)
ES (1) ES2915576T3 (da)
IL (3) IL313387A (da)
SG (2) SG11202002479XA (da)
WO (2) WO2019058253A1 (da)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018336128B2 (en) 2017-09-19 2023-01-05 Tropic Biosciences UK Limited Modifying the specificity of plant non-coding RNA molecules for silencing gene expression
WO2019222545A1 (en) 2018-05-16 2019-11-21 Synthego Corporation Methods and systems for guide rna design and use
CN110857438B (zh) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 一种高效产生siRNA的烟草花叶病毒基因片段、弱毒疫苗、制备方法及其应用
EP3931321A1 (en) * 2019-03-01 2022-01-05 Syngenta Crop Protection AG Suppression of target gene expression through genome editing of native mirnas
GB201903519D0 (en) * 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
GB201903520D0 (en) * 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
CN110551719B (zh) * 2019-07-30 2023-06-02 中山大学 长链非编码rna基因alex1及其在提高水稻白叶枯病抗性中的应用
CN111508558B (zh) * 2020-03-23 2021-12-14 广州赛业百沐生物科技有限公司 一种基于CRISPR-Cas9技术设计点突变模型的方法及系统
CN111676220B (zh) * 2020-05-21 2022-03-29 扬州大学 杨树长链非编码RNA lnc11及其应用
US20240415939A1 (en) * 2020-06-30 2024-12-19 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions for genome editing and methods of use thereof
CN114073760B (zh) * 2020-08-21 2024-02-13 北京大学 Rdr蛋白在肿瘤治疗中的应用
US20240000970A1 (en) * 2020-12-01 2024-01-04 Emendobio Inc. Differential knockout of a heterozygous allele of lrrk2
CN112359049B (zh) * 2020-12-10 2022-01-28 昆明理工大学 一种岷江百合几丁质酶基因LrCHI2及其应用
CN112795568B (zh) * 2021-01-06 2023-07-18 河南农业大学 一种控制玉米雌穗穗粗杂种优势的非编码基因的应用及其前体序列
GB202103256D0 (en) * 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes
CN114134155B (zh) * 2021-06-29 2023-09-12 中国农业科学院油料作物研究所 一种mlo基因突变体及其制备方法与应用
CN113444727B (zh) * 2021-06-30 2022-06-21 中国热带农业科学院热带生物技术研究所 一种LncRNA及其应用
KR20240031315A (ko) 2021-07-02 2024-03-07 트로픽 바이오사이언시즈 유케이 리미티드 바나나 과실의 갈변 지연 또는 예방
KR102574819B1 (ko) * 2021-08-09 2023-09-04 경상국립대학교산학협력단 P34와 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도
KR102573947B1 (ko) * 2021-08-09 2023-09-01 경상국립대학교산학협력단 콩 유전자교정 효율 증대를 위한 유전자교정 시스템 및 이의 용도
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