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IL271553A - Variants of t7 RNA polymerase - Google Patents

Variants of t7 RNA polymerase

Info

Publication number
IL271553A
IL271553A IL271553A IL27155319A IL271553A IL 271553 A IL271553 A IL 271553A IL 271553 A IL271553 A IL 271553A IL 27155319 A IL27155319 A IL 27155319A IL 271553 A IL271553 A IL 271553A
Authority
IL
Israel
Prior art keywords
variants
rna polymerase
polymerase
rna
Prior art date
Application number
IL271553A
Other languages
Hebrew (he)
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of IL271553A publication Critical patent/IL271553A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IL271553A 2017-06-30 2019-12-18 Variants of t7 RNA polymerase IL271553A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762527764P 2017-06-30 2017-06-30
US201762528846P 2017-07-05 2017-07-05
PCT/US2018/038288 WO2019005540A1 (en) 2017-06-30 2018-06-19 T7 rna polymerase variants

Publications (1)

Publication Number Publication Date
IL271553A true IL271553A (en) 2020-02-27

Family

ID=64734780

Family Applications (1)

Application Number Title Priority Date Filing Date
IL271553A IL271553A (en) 2017-06-30 2019-12-18 Variants of t7 RNA polymerase

Country Status (9)

Country Link
US (1) US10793841B2 (en)
EP (1) EP3645711A4 (en)
JP (2) JP7588391B2 (en)
KR (2) KR20250028535A (en)
CN (2) CN119162145A (en)
AU (2) AU2018292105B2 (en)
CA (1) CA3066642A1 (en)
IL (1) IL271553A (en)
WO (1) WO2019005540A1 (en)

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ES2983060T3 (en) 2017-08-18 2024-10-21 Modernatx Inc RNA polymerase variants
US11072808B2 (en) 2018-10-04 2021-07-27 New England Biolabs, Inc. Methods and compositions for increasing capping efficiency of transcribed RNA
CN113795579A (en) 2019-02-20 2021-12-14 摩登纳特斯有限公司 RNA polymerase variants for co-transcriptional capping
US11851694B1 (en) 2019-02-20 2023-12-26 Modernatx, Inc. High fidelity in vitro transcription
US20220325259A1 (en) * 2020-04-30 2022-10-13 Daan Gene Co., Ltd. A heat-resistant dna polymerase mutant with high amplification activity
US20240376447A1 (en) * 2023-05-12 2024-11-14 Codexis, Inc. Engineered rna polymerase variants
US20240376449A1 (en) * 2023-05-12 2024-11-14 Codexis, Inc. Rna polymerase variants
CN118222535B (en) * 2023-12-28 2025-01-14 翌圣生物科技(上海)股份有限公司 T7 RNA polymerase mutants and their applications
GB202406294D0 (en) 2024-05-06 2024-06-19 Thermo Fisher Scientific Baltics Uab Methods and compositions for producing capped mRNA

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Also Published As

Publication number Publication date
CN111032863B (en) 2024-10-22
RU2020103726A3 (en) 2022-04-07
JP7588391B2 (en) 2024-11-22
AU2018292105A1 (en) 2019-12-19
CA3066642A1 (en) 2019-01-03
AU2018292105B2 (en) 2024-12-12
JP2023159437A (en) 2023-10-31
KR102773542B1 (en) 2025-02-25
CN111032863A (en) 2020-04-17
RU2020103726A (en) 2021-07-30
KR20250028535A (en) 2025-02-28
EP3645711A1 (en) 2020-05-06
JP2020530266A (en) 2020-10-22
WO2019005540A1 (en) 2019-01-03
CN119162145A (en) 2024-12-20
US10793841B2 (en) 2020-10-06
KR20200023455A (en) 2020-03-04
US20190002851A1 (en) 2019-01-03
EP3645711A4 (en) 2021-04-21
AU2025201307A1 (en) 2025-03-20

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