DK3585886T5 - Storskalasyntese af messenger-rna - Google Patents
Storskalasyntese af messenger-rna Download PDFInfo
- Publication number
- DK3585886T5 DK3585886T5 DK18713747.6T DK18713747T DK3585886T5 DK 3585886 T5 DK3585886 T5 DK 3585886T5 DK 18713747 T DK18713747 T DK 18713747T DK 3585886 T5 DK3585886 T5 DK 3585886T5
- Authority
- DK
- Denmark
- Prior art keywords
- messenger rna
- scale synthesis
- synthesis
- scale
- messenger
- Prior art date
Links
- 230000015572 biosynthetic process Effects 0.000 title 1
- 108020004999 messenger RNA Proteins 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
- G01N27/44778—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis on a common gel carrier, i.e. 2D gel electrophoresis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Electrochemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762464043P | 2017-02-27 | 2017-02-27 | |
| PCT/US2018/020008 WO2018157153A1 (en) | 2017-02-27 | 2018-02-27 | Large scale synthesis of messenger rna |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK3585886T3 DK3585886T3 (da) | 2024-02-19 |
| DK3585886T5 true DK3585886T5 (da) | 2024-09-02 |
Family
ID=61800667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18713747.6T DK3585886T5 (da) | 2017-02-27 | 2018-02-27 | Storskalasyntese af messenger-rna |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US11560562B2 (da) |
| EP (2) | EP3585886B1 (da) |
| AU (2) | AU2018224325B2 (da) |
| CA (1) | CA3053814A1 (da) |
| DK (1) | DK3585886T5 (da) |
| ES (1) | ES2971902T3 (da) |
| HU (1) | HUE065140T2 (da) |
| IL (2) | IL268892B2 (da) |
| MA (1) | MA47607A (da) |
| WO (1) | WO2018157153A1 (da) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2795110T3 (es) | 2011-06-08 | 2020-11-20 | Translate Bio Inc | Lípidos escindibles |
| WO2019104152A1 (en) | 2017-11-22 | 2019-05-31 | Modernatx, Inc. | Polynucleotides encoding ornithine transcarbamylase for the treatment of urea cycle disorders |
| AU2020405214A1 (en) | 2019-12-20 | 2022-08-11 | Translate Bio, Inc. | Improved process of preparing mRNA-loaded lipid nanoparticles |
| CA3162368A1 (en) | 2019-12-20 | 2021-06-24 | Shrirang KARVE | Rectal delivery of messenger rna |
| AU2021218671A1 (en) | 2020-02-10 | 2022-10-06 | Translate Bio, Inc. | Methods and compositions for messenger RNA purification |
| EP4107733A1 (en) | 2020-02-18 | 2022-12-28 | Translate Bio, Inc. | Improved processes for in vitro transcription of messenger rna |
| EP4110296A1 (en) | 2020-02-25 | 2023-01-04 | Translate Bio, Inc. | Improved processes of preparing mrna-loaded lipid nanoparticles |
| EP4146680A1 (en) | 2020-05-07 | 2023-03-15 | Translate Bio, Inc. | Composition and methods for treatment of primary ciliary dyskinesia |
| JP2023524767A (ja) | 2020-05-07 | 2023-06-13 | トランスレイト バイオ， インコーポレイテッド | ＳＡＲＳ－ＣｏＶ－２抗原をコードする最適化されたヌクレオチド配列 |
| WO2021231901A1 (en) | 2020-05-15 | 2021-11-18 | Translate Bio, Inc. | Lipid nanoparticle formulations for mrna delivery |
| CA3194863A1 (en) | 2020-10-06 | 2022-04-14 | Shrirang KARVE | Improved process and formulation of lipid nanoparticles |
| IL301973A (en) | 2020-10-12 | 2023-06-01 | Translate Bio Inc | Improved process of preparing mrna-loaded lipid nanoparticles |
| US20220133631A1 (en) | 2020-10-12 | 2022-05-05 | Translate Bio, Inc. | Process of preparing ice-based lipid nanoparticles |
| CN116438298A (zh) * | 2020-10-15 | 2023-07-14 | 翻译生物公司 | 信使rna的大规模合成 |
| WO2022099003A1 (en) * | 2020-11-06 | 2022-05-12 | Sanofi | Lipid nanoparticles for delivering mrna vaccines |
| EP4313115A1 (en) | 2021-03-25 | 2024-02-07 | Translate Bio, Inc. | Optimized nucleotide sequences encoding the extracellular domain of human ace2 protein or a portion thereof |
| US20250073351A1 (en) | 2021-04-19 | 2025-03-06 | Translate Bio, Inc. | Improved compositions for delivery of mrna |
| WO2023278754A1 (en) | 2021-07-01 | 2023-01-05 | Translate Bio, Inc. | Compositions for delivery of mrna |
| CN118591398A (zh) | 2021-11-10 | 2024-09-03 | 翻译生物公司 | 用于治疗原发性纤毛运动不良症的组合物和方法 |
| AU2023301074A1 (en) * | 2022-07-01 | 2024-12-19 | Arcturus Therapeutics, Inc. | Improved methods for in vitro transcription |
| WO2024023335A1 (en) | 2022-07-29 | 2024-02-01 | Sanofi | Methods for ethanol-free mrna purification |
| WO2024112652A1 (en) | 2022-11-21 | 2024-05-30 | Translate Bio, Inc. | Compositions of dry powder formulations of messenger rna and methods of use thereof |
| WO2024126809A1 (en) | 2022-12-15 | 2024-06-20 | Sanofi | Mrna encoding influenza virus-like particle |
| TW202438514A (zh) | 2022-12-20 | 2024-10-01 | 法商賽諾菲公司 | 鼻病毒mrna疫苗 |
| WO2024133884A2 (en) | 2022-12-23 | 2024-06-27 | Sanofi | Optimized tailing of messenger rna |
| WO2024141786A2 (en) | 2022-12-29 | 2024-07-04 | Popvax Private Limited | Multitarget vaccines and therapeutics |
| WO2024141784A2 (en) | 2022-12-29 | 2024-07-04 | Popvax Private Limited | Broadly protective betacoronavirus vaccines and compositions |
| WO2024184489A1 (en) | 2023-03-07 | 2024-09-12 | Sanofi | Manufacture of messenger rna with kp34 polymerase |
| WO2024218166A1 (en) | 2023-04-17 | 2024-10-24 | Sanofi | Reconstitutable dry powder formulations and methods of use thereof |
| WO2025017151A1 (en) | 2023-07-18 | 2025-01-23 | Sanofi | Stable poly(a)-encoding messenger rna templates |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL2578685T3 (pl) | 2005-08-23 | 2020-01-31 | The Trustees Of The University Of Pennsylvania | Rna zawierający zmodyfikowane nukleozydy i sposoby jego zastosowania |
| WO2009030254A1 (en) | 2007-09-04 | 2009-03-12 | Curevac Gmbh | Complexes of rna and cationic peptides for transfection and for immunostimulation |
| DK2459231T3 (da) | 2009-07-31 | 2016-09-05 | Ethris Gmbh | Rna med en kombination af umodificerede og modificerede nukleotider til proteinekspression |
| US8326547B2 (en) | 2009-10-07 | 2012-12-04 | Nanjingjinsirui Science & Technology Biology Corp. | Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm |
| ES2666559T3 (es) | 2009-12-01 | 2018-05-07 | Translate Bio, Inc. | Entrega del mrna para la aumentación de proteínas y enzimas en enfermedades genéticas humanas |
| US8853377B2 (en) | 2010-11-30 | 2014-10-07 | Shire Human Genetic Therapies, Inc. | mRNA for use in treatment of human genetic diseases |
| DK3586861T3 (da) * | 2011-06-08 | 2022-04-25 | Translate Bio Inc | Lipidnanopartikelsammensætninger og fremgangsmåder til mrna-indgivelse |
| MX2015011944A (es) | 2013-03-14 | 2015-12-01 | Shire Human Genetic Therapies | Evaluacion cuantitativa para la eficacia de los casquetes de arn mensajero. |
| EP2970955B1 (en) | 2013-03-14 | 2018-11-14 | Translate Bio, Inc. | Methods for purification of messenger rna |
| EP3636742A1 (en) | 2014-04-25 | 2020-04-15 | Translate Bio, Inc. | Methods for purification of messenger rna |
| AU2015273933B2 (en) * | 2014-06-10 | 2021-02-11 | CureVac Manufacturing GmbH | Methods and means for enhancing RNA production |
| US10172924B2 (en) | 2015-03-19 | 2019-01-08 | Translate Bio, Inc. | MRNA therapy for pompe disease |
| DK3294885T3 (da) * | 2015-05-08 | 2020-08-10 | Curevac Real Estate Gmbh | Fremgangsmåde til at fremstille rna |
-
2018
- 2018-02-27 EP EP18713747.6A patent/EP3585886B1/en active Active
- 2018-02-27 IL IL268892A patent/IL268892B2/en unknown
- 2018-02-27 US US15/907,163 patent/US11560562B2/en active Active
- 2018-02-27 ES ES18713747T patent/ES2971902T3/es active Active
- 2018-02-27 EP EP22169158.7A patent/EP4105326A1/en active Pending
- 2018-02-27 AU AU2018224325A patent/AU2018224325B2/en active Active
- 2018-02-27 HU HUE18713747A patent/HUE065140T2/hu unknown
- 2018-02-27 IL IL310939A patent/IL310939A/en unknown
- 2018-02-27 CA CA3053814A patent/CA3053814A1/en active Pending
- 2018-02-27 WO PCT/US2018/020008 patent/WO2018157153A1/en unknown
- 2018-02-27 DK DK18713747.6T patent/DK3585886T5/da active
- 2018-02-27 MA MA047607A patent/MA47607A/fr unknown
-
2022
- 2022-12-14 US US18/065,952 patent/US20240018514A1/en active Pending
-
2024
- 2024-07-31 AU AU2024205244A patent/AU2024205244A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3585886B1 (en) | 2023-11-22 |
| EP4105326A1 (en) | 2022-12-21 |
| AU2024205244A1 (en) | 2024-08-22 |
| MA47607A (fr) | 2020-01-01 |
| IL310939A (en) | 2024-04-01 |
| US20240018514A1 (en) | 2024-01-18 |
| AU2018224325B2 (en) | 2024-05-02 |
| US20180258423A1 (en) | 2018-09-13 |
| HUE065140T2 (hu) | 2024-05-28 |
| DK3585886T3 (da) | 2024-02-19 |
| CA3053814A1 (en) | 2018-08-30 |
| AU2018224325A1 (en) | 2019-09-12 |
| IL268892B2 (en) | 2024-07-01 |
| WO2018157153A1 (en) | 2018-08-30 |
| EP3585886A1 (en) | 2020-01-01 |
| ES2971902T3 (es) | 2024-06-10 |
| IL268892A (en) | 2019-10-31 |
| US11560562B2 (en) | 2023-01-24 |
| IL268892B1 (en) | 2024-03-01 |
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