DK3250703T3 - Ligeringsanalyser i væskefase - Google Patents
Ligeringsanalyser i væskefase Download PDFInfo
- Publication number
- DK3250703T3 DK3250703T3 DK16744000.7T DK16744000T DK3250703T3 DK 3250703 T3 DK3250703 T3 DK 3250703T3 DK 16744000 T DK16744000 T DK 16744000T DK 3250703 T3 DK3250703 T3 DK 3250703T3
- Authority
- DK
- Denmark
- Prior art keywords
- liquid
- analysis
- phase
- liquid phase
- liquid analysis
- Prior art date
Links
- 239000007788 liquid Substances 0.000 title 1
- 239000007791 liquid phase Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/307—Single strand endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562108161P | 2015-01-27 | 2015-01-27 | |
| US14/788,670 US9856521B2 (en) | 2015-01-27 | 2015-06-30 | Ligation assays in liquid phase |
| PCT/US2016/014999 WO2016123154A1 (en) | 2015-01-27 | 2016-01-26 | Ligation assays in liquid phase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3250703T3 true DK3250703T3 (da) | 2022-06-27 |
Family
ID=56544256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16744000.7T DK3250703T3 (da) | 2015-01-27 | 2016-01-26 | Ligeringsanalyser i væskefase |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9856521B2 (da) |
| EP (2) | EP4134441A1 (da) |
| JP (1) | JP6768706B2 (da) |
| CN (1) | CN108112258A (da) |
| AU (1) | AU2016211632B9 (da) |
| CA (1) | CA2975088A1 (da) |
| DK (1) | DK3250703T3 (da) |
| ES (1) | ES2922645T3 (da) |
| GB (2) | GB2542929B (da) |
| PT (1) | PT3250703T (da) |
| SG (1) | SG11201706086XA (da) |
| WO (1) | WO2016123154A1 (da) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10648030B2 (en) | 2012-01-13 | 2020-05-12 | Affymetrix, Inc. | Methods of determining the presence or absence of a plurality of target polynucleotides in a sample |
| EP3511423B2 (en) | 2012-10-17 | 2024-05-29 | Spatial Transcriptomics AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| US11091810B2 (en) * | 2015-01-27 | 2021-08-17 | BioSpyder Technologies, Inc. | Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology |
| US10683534B2 (en) | 2015-01-27 | 2020-06-16 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
| US20230313309A1 (en) * | 2014-09-08 | 2023-10-05 | BioSpyder Technologies, Inc. | Methods for Nucleic Acid Sequence Detection |
| SG11201707515SA (en) | 2015-04-10 | 2017-10-30 | Spatial Transcriptomics Ab | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US11118216B2 (en) * | 2015-09-08 | 2021-09-14 | Affymetrix, Inc. | Nucleic acid analysis by joining barcoded polynucleotide probes |
| EP3565906B1 (en) | 2017-01-05 | 2021-01-27 | Tervisetehnoloogiate Arenduskeskus AS | Quantifying dna sequences |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| US20220025447A1 (en) | 2018-12-10 | 2022-01-27 | 10X Genomics, Inc. | Generating spatial arrays with gradients |
| WO2020123406A1 (en) * | 2018-12-10 | 2020-06-18 | Combinati Incorporated | Microfluidic array for sample digitization |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11345967B2 (en) | 2019-05-23 | 2022-05-31 | Paradigm Diagnostics | Tissue preparation using nuclease |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| AU2020412766C1 (en) | 2019-12-23 | 2025-01-02 | 10X Genomics, Inc. | Methods for spatial analysis using RNA-templated ligation |
| CN115038794A (zh) | 2019-12-23 | 2022-09-09 | 10X基因组学有限公司 | 在基于分区的测定中使用固定生物样品的组合物和方法 |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| US12129516B2 (en) | 2020-02-07 | 2024-10-29 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| CN115916999A (zh) | 2020-04-22 | 2023-04-04 | 10X基因组学有限公司 | 用于使用靶向rna耗竭进行空间分析的方法 |
| ES2989052T3 (es) | 2020-05-22 | 2024-11-25 | 10X Genomics Inc | Medición espacio-temporal simultánea de la expresión génica y la actividad celular |
| EP4153776B1 (en) | 2020-05-22 | 2025-03-05 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4162074B1 (en) | 2020-06-08 | 2024-04-24 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| EP4165207B1 (en) | 2020-06-10 | 2024-09-25 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
| WO2021263111A1 (en) | 2020-06-25 | 2021-12-30 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| EP4060049B1 (en) | 2021-03-18 | 2023-08-23 | Genomill Health Oy | Methods for accurate parallel quantification of nucleic acids in dilute or non-purified samples |
| FI4060050T3 (fi) | 2021-03-18 | 2023-05-04 | Genomill Health Oy | Erittäin herkät menetelmät nukleiinihappojen tarkkaan rinnakkaiseen kvantifiointiin |
| WO2022221425A1 (en) | 2021-04-14 | 2022-10-20 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| ES3011462T3 (en) | 2021-09-01 | 2025-04-07 | 10X Genomics Inc | Methods for blocking a capture probe on a spatial array |
| EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| EP4215619A1 (en) * | 2022-01-21 | 2023-07-26 | Genomill Health Oy | Methods for sensitive and accurate parallel quantification of nucleic acids |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5225324A (en) * | 1987-04-24 | 1993-07-06 | Bioscience International, Inc. | Diagnostics for mycobacteria in public health, medical, and veterinary practice |
| US4988617A (en) * | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| US7045289B2 (en) * | 1991-09-09 | 2006-05-16 | Third Wave Technologies, Inc. | Detection of RNA Sequences |
| SE9400522D0 (sv) * | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| JP4040087B2 (ja) * | 1995-05-05 | 2008-01-30 | アプレラ コーポレイション | Ｐｃｒ増幅とハイブリダイゼーションプロービングアッセイとを組み合わせた方法およびそのための試薬 |
| EP0876510B1 (en) * | 1995-12-22 | 2008-05-07 | Dade Behring Marburg GmbH | Homogeneous amplification and detection of nucleic acids |
| CA2255774C (en) | 1996-05-29 | 2008-03-18 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
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| US7361489B2 (en) * | 2001-10-15 | 2008-04-22 | Mount Sinai School Of Medicine Of New York University | Nucleic acid amplification methods |
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| DK1633888T3 (da) * | 2003-06-17 | 2009-07-13 | Keygene Nv | Midler og fremgangsmåde til påvisning af målnucleotidsekvenser under anvendelse af ligeringsassays med forbedrede oligonucleotidprobepar |
| ATE457363T1 (de) | 2004-12-29 | 2010-02-15 | Applied Biosystems Llc | Verfahren, zusammensetzungen und kits zur bildung einander ergänzender polynukleotide |
| CA2595729A1 (en) | 2005-01-21 | 2006-07-27 | Third Wave Technologies, Inc. | Methods and compositions for increased dynamic range detection of nucleic acid molecules |
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| US9487839B2 (en) | 2009-09-29 | 2016-11-08 | Case Western Reserve University | Method for detecting single nucleotide polymorphisms |
| US10066257B2 (en) * | 2010-03-29 | 2018-09-04 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
| EP2569453B1 (en) * | 2010-05-14 | 2015-12-16 | Fluidigm Corporation | Nucleic acid isolation methods |
| US20130261003A1 (en) | 2010-08-06 | 2013-10-03 | Ariosa Diagnostics, In. | Ligation-based detection of genetic variants |
| CN103649335B (zh) | 2011-05-04 | 2015-11-25 | Htg分子诊断有限公司 | 定量核酸酶保护测定（qnpa）和测序（qnps）的改进 |
| GB201116131D0 (en) * | 2011-09-19 | 2011-11-02 | Epistem Ltd | Probe |
| ES2655509T3 (es) * | 2012-02-14 | 2018-02-20 | Cornell University | Método para la cuantificación relativa de la secuencia, la expresión o los cambios de copias de ácidos nucleicos, usando reacciones combinadas de polimerasa, ligadura y nucleasa |
| EP2893034B9 (en) | 2012-09-10 | 2018-04-18 | Genesky Diagnostics (Suzhou) Inc. | Method for multiplex nucleic acid analysis |
| KR101984700B1 (ko) * | 2012-11-19 | 2019-05-31 | 삼성전자주식회사 | 폴리뉴클레오티드 및 그의 용도 |
| CN105392897B (zh) * | 2013-03-19 | 2020-03-20 | 定向基因组学公司 | 靶序列的富集 |
| EP3004367A4 (en) | 2013-06-07 | 2017-02-22 | Athena Diagnostics Inc. | Molecular barcoding for multiplex sequencing |
| US10683534B2 (en) | 2015-01-27 | 2020-06-16 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
| US9938566B2 (en) | 2014-09-08 | 2018-04-10 | BioSpyder Technologies, Inc. | Profiling expression at transcriptome scale |
| US9957550B2 (en) | 2014-09-08 | 2018-05-01 | BioSpyder Technologies, Inc. | Attenuators |
-
2015
- 2015-06-30 US US14/788,670 patent/US9856521B2/en active Active
-
2016
- 2016-01-26 ES ES16744000T patent/ES2922645T3/es active Active
- 2016-01-26 JP JP2017558365A patent/JP6768706B2/ja active Active
- 2016-01-26 CA CA2975088A patent/CA2975088A1/en active Pending
- 2016-01-26 AU AU2016211632A patent/AU2016211632B9/en active Active
- 2016-01-26 DK DK16744000.7T patent/DK3250703T3/da active
- 2016-01-26 CN CN201680018676.0A patent/CN108112258A/zh active Pending
- 2016-01-26 GB GB1614871.0A patent/GB2542929B/en active Active
- 2016-01-26 PT PT167440007T patent/PT3250703T/pt unknown
- 2016-01-26 SG SG11201706086XA patent/SG11201706086XA/en unknown
- 2016-01-26 EP EP22173117.7A patent/EP4134441A1/en active Pending
- 2016-01-26 GB GB1707052.5A patent/GB2547369A/en not_active Withdrawn
- 2016-01-26 WO PCT/US2016/014999 patent/WO2016123154A1/en active Application Filing
- 2016-01-26 EP EP16744000.7A patent/EP3250703B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2975088A1 (en) | 2016-08-04 |
| GB2542929B (en) | 2017-08-02 |
| US20160222447A1 (en) | 2016-08-04 |
| US9856521B2 (en) | 2018-01-02 |
| GB201707052D0 (en) | 2017-06-14 |
| EP3250703B1 (en) | 2022-06-08 |
| GB201614871D0 (en) | 2016-10-19 |
| EP4134441A1 (en) | 2023-02-15 |
| AU2016211632B2 (en) | 2021-03-11 |
| JP6768706B2 (ja) | 2020-10-14 |
| CN108112258A (zh) | 2018-06-01 |
| GB2542929A (en) | 2017-04-05 |
| SG11201706086XA (en) | 2017-08-30 |
| PT3250703T (pt) | 2022-09-19 |
| AU2016211632B9 (en) | 2021-03-25 |
| EP3250703A1 (en) | 2017-12-06 |
| JP2018503403A (ja) | 2018-02-08 |
| GB2547369A (en) | 2017-08-16 |
| WO2016123154A1 (en) | 2016-08-04 |
| AU2016211632A1 (en) | 2017-08-24 |
| EP3250703A4 (en) | 2018-08-01 |
| ES2922645T3 (es) | 2022-09-19 |
| AU2016211632A2 (en) | 2017-08-31 |
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