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DK3188836T3 - Dna-syntese - Google Patents

Dna-syntese Download PDF

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Publication number
DK3188836T3
DK3188836T3 DK15756228.1T DK15756228T DK3188836T3 DK 3188836 T3 DK3188836 T3 DK 3188836T3 DK 15756228 T DK15756228 T DK 15756228T DK 3188836 T3 DK3188836 T3 DK 3188836T3
Authority
DK
Denmark
Prior art keywords
dna synthesis
dna
synthesis
Prior art date
Application number
DK15756228.1T
Other languages
English (en)
Inventor
Neil Porter
Paul Rothwell
Jonathan Extance
Original Assignee
Touchlight Ip Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Touchlight Ip Ltd filed Critical Touchlight Ip Ltd
Application granted granted Critical
Publication of DK3188836T3 publication Critical patent/DK3188836T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/113Telomerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/109Pressure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/153Viscosity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119Strand displacement amplification [SDA]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125Rolling circle

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK15756228.1T 2014-09-05 2015-08-21 Dna-syntese DK3188836T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1415789.5A GB201415789D0 (en) 2014-09-05 2014-09-05 Synthesis of DNA
PCT/GB2015/052434 WO2016034849A1 (en) 2014-09-05 2015-08-21 Synthesis of dna

Publications (1)

Publication Number Publication Date
DK3188836T3 true DK3188836T3 (da) 2024-03-11

Family

ID=51796300

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15756228.1T DK3188836T3 (da) 2014-09-05 2015-08-21 Dna-syntese

Country Status (18)

Country Link
US (1) US10501782B1 (da)
EP (1) EP3188836B1 (da)
JP (1) JP6708631B2 (da)
KR (1) KR102497228B1 (da)
CN (1) CN107075577B (da)
AU (1) AU2015310728B2 (da)
BR (1) BR112017003836B1 (da)
DK (1) DK3188836T3 (da)
ES (1) ES2973011T3 (da)
FI (1) FI3188836T3 (da)
GB (1) GB201415789D0 (da)
IL (1) IL250612B (da)
LT (1) LT3188836T (da)
MX (1) MX2017002785A (da)
RU (1) RU2714253C2 (da)
SG (1) SG11201701616PA (da)
WO (1) WO2016034849A1 (da)
ZA (1) ZA201701246B (da)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10077459B2 (en) 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
CN116732145A (zh) 2016-08-16 2023-09-12 塔驰莱特Ip有限公司 闭合线性dna的生产
US11001868B2 (en) 2017-08-11 2021-05-11 Global Life Sciences Solutions Operations UK Ltd Cell-free protein expression using double-stranded concatameric DNA
GB201813429D0 (en) * 2018-08-17 2018-10-03 Touchlight Ip Ltd Synthesis of DNA with improved yield
EP3792367A1 (en) 2019-09-11 2021-03-17 Universität Bielefeld Method for the production of raav and method for the in vitro generation of genetically engineered, linear, single-stranded nucleic acid fragments containing itr sequences flanking a gene of interest
GB202002068D0 (en) * 2020-02-14 2020-04-01 Touchlight Ip Ltd DNA synthesis yield improvements
GB202007428D0 (en) 2020-05-19 2020-07-01 Fabricnano Ltd Polynucleotide synthesis
US11720801B2 (en) 2020-08-25 2023-08-08 International Business Machines Corporation Chemical reaction network for estimating concentration of chemical species based on an identified pattern of output chemical species
CN117858959A (zh) * 2021-05-10 2024-04-09 加利福尼亚太平洋生物科学股份有限公司 滚环扩增期间的dna扩增缓冲液补充
WO2023009842A1 (en) 2021-07-30 2023-02-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
GB202111742D0 (en) 2021-08-16 2021-09-29 Touchlight Ip Ltd Improved process
GB202114105D0 (en) 2021-10-01 2021-11-17 Fabricnano Ltd Nucleotide synthesis
WO2023069948A1 (en) 2021-10-18 2023-04-27 Flagship Pioneering Innovations Vii, Llc Dna compositions and related methods
JP2025501740A (ja) 2021-12-20 2025-01-23 ゾエティス・サービシーズ・エルエルシー ワクチンにおけるアジュバントとしてのインターフェロンの使用
CN118414349A (zh) 2021-12-20 2024-07-30 硕腾服务有限责任公司 用于在细胞膜上表达抗原的组合物和方法
WO2023122525A1 (en) 2021-12-20 2023-06-29 Zoetis Services Llc Compositions and methods for prevention of piscine myocarditis
EP4293101A1 (en) 2022-06-14 2023-12-20 Asklepios Biopharmaceutical, Inc. Reactor with temperature control and method of using the same
JP2025530305A (ja) 2022-09-12 2025-09-11 ゾエティス・サービシーズ・エルエルシー 水産養殖用ワクチンの投与方法
WO2024115669A1 (en) * 2022-11-30 2024-06-06 Evaxion Biotech A/S Process for production of synthetic circular dna
DK202370610A1 (en) 2022-12-13 2024-07-12 Zoetis Services Llc Methods of vaccine administration to salmonids
WO2024191147A1 (ko) * 2023-03-10 2024-09-19 고려대학교 산학협력단 아토리터 방울 기반 전기화학 병렬 dna 합성기 및 이를 이용한 dna 합성 방법
US11981936B1 (en) 2023-09-29 2024-05-14 New England Biolabs, Inc. TelA variants, compositions, and methods
WO2025264964A1 (en) 2024-06-20 2025-12-26 Zoetis Services Llc Vaccines against tenacibaculum finnmarkense

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6953676B1 (en) 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
DE69839709D1 (de) 1997-12-24 2008-08-21 Cepheid Vorrichtung und Verfahren zur Lyse
RU2218414C2 (ru) * 2001-05-04 2003-12-10 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ интеграции множественных полимеразных реакций амплификации с последующим анализом амплифицированных последовательностей непосредственно на биочипе
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
US7081339B2 (en) 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
AU2003251905A1 (en) 2002-07-12 2004-02-02 Affymetrix, Inc. Synthetic tag genes
DE602004021235D1 (de) 2003-02-21 2009-07-09 Geneform Technologies Ltd Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
EP1885880B1 (en) * 2005-04-29 2010-07-21 Synthetic Genomics, Inc. Amplification and cloning of single dna molecules using rolling circle amplification
CA2713101C (en) 2006-10-06 2016-09-13 Vandalia Research, Inc. Method for a continuous rapid thermal cycle system
GB201013153D0 (en) * 2010-08-04 2010-09-22 Touchlight Genetics Ltd Primer for production of closed linear DNA
US9114399B2 (en) 2010-08-31 2015-08-25 Canon U.S. Life Sciences, Inc. System and method for serial processing of multiple nucleic acid assays
CN102000478A (zh) * 2010-10-29 2011-04-06 中国农业大学 一种除湿液再生方法及装置
WO2012061444A2 (en) * 2010-11-01 2012-05-10 Hiddessen Amy L System for forming emulsions
CN103255227B (zh) * 2013-05-30 2015-02-25 上海快灵生物科技有限公司 引物介导环化的恒温核酸滚环扩增方法及试剂盒

Also Published As

Publication number Publication date
IL250612A0 (en) 2017-04-30
KR102497228B1 (ko) 2023-02-07
RU2714253C2 (ru) 2020-02-13
CN107075577A (zh) 2017-08-18
AU2015310728B2 (en) 2021-09-16
KR20170045349A (ko) 2017-04-26
ES2973011T3 (es) 2024-06-18
WO2016034849A1 (en) 2016-03-10
IL250612B (en) 2021-01-31
RU2017111097A (ru) 2018-10-05
EP3188836A1 (en) 2017-07-12
CN107075577B (zh) 2022-01-11
RU2017111097A3 (da) 2018-10-05
CA2959974A1 (en) 2016-03-10
FI3188836T3 (fi) 2024-02-21
US10501782B1 (en) 2019-12-10
BR112017003836A2 (pt) 2018-04-10
JP6708631B2 (ja) 2020-06-10
EP3188836B1 (en) 2023-12-20
MX2017002785A (es) 2017-09-28
AU2015310728A1 (en) 2017-03-09
SG11201701616PA (en) 2017-03-30
GB201415789D0 (en) 2014-10-22
BR112017003836B1 (pt) 2022-04-05
LT3188836T (lt) 2024-03-25
ZA201701246B (en) 2021-05-26
JP2017525384A (ja) 2017-09-07
NZ729389A (en) 2024-07-05

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