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DK3183340T3 - Termolabile exonukleaser - Google Patents

Termolabile exonukleaser Download PDF

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Publication number
DK3183340T3
DK3183340T3 DK15770444.6T DK15770444T DK3183340T3 DK 3183340 T3 DK3183340 T3 DK 3183340T3 DK 15770444 T DK15770444 T DK 15770444T DK 3183340 T3 DK3183340 T3 DK 3183340T3
Authority
DK
Denmark
Prior art keywords
termolabile
exonucleaser
termolabile exonucleaser
Prior art date
Application number
DK15770444.6T
Other languages
English (en)
Inventor
Marit Sjo Lorentzen
Yvonne Piotrowski
Nils Peder Willassen
Terese Solstad
Atle Noralf Larsen
Elisabeth Lill Andreassen
Morten Elde
Olav Lanes
Original Assignee
Arcticzymes As
Univ I Tromsoe Norges Arktiske Univ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arcticzymes As, Univ I Tromsoe Norges Arktiske Univ filed Critical Arcticzymes As
Application granted granted Critical
Publication of DK3183340T3 publication Critical patent/DK3183340T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/11Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
    • C12Y301/11001Exodeoxyribonuclease I (3.1.11.1)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/30Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/325Single stranded exonuclease
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/916Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922Ribonucleases (RNAses); Deoxyribonucleases (DNAses)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK15770444.6T 2014-08-19 2015-08-19 Termolabile exonukleaser DK3183340T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1414745.8A GB201414745D0 (en) 2014-08-19 2014-08-19 Exonucleases
PCT/EP2015/001703 WO2016026574A1 (en) 2014-08-19 2015-08-19 Thermolabile exonucleases

Publications (1)

Publication Number Publication Date
DK3183340T3 true DK3183340T3 (da) 2019-08-19

Family

ID=51662672

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15770444.6T DK3183340T3 (da) 2014-08-19 2015-08-19 Termolabile exonukleaser

Country Status (12)

Country Link
US (3) US10087483B2 (da)
EP (1) EP3183340B1 (da)
JP (1) JP6316505B2 (da)
KR (2) KR102432501B1 (da)
CN (1) CN107109383B (da)
CA (1) CA2956853C (da)
DK (1) DK3183340T3 (da)
ES (1) ES2742186T3 (da)
GB (1) GB201414745D0 (da)
LT (1) LT3183340T (da)
PL (1) PL3183340T3 (da)
WO (1) WO2016026574A1 (da)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018013250A1 (en) * 2016-07-14 2018-01-18 Gene Infinity Llc Methods of rapid ligation-independent cloning of dna and uses thereof
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
GB201721053D0 (en) * 2017-12-15 2018-01-31 Univ I Tromsø - Norges Arktiske Univ DNA polymerases
AU2020222888B2 (en) 2019-02-11 2024-08-01 Ultima Genomics, Inc. Methods for nucleic acid analysis
WO2020172362A1 (en) * 2019-02-20 2020-08-27 Genetics Research, Llc Regulated multiplex reactions in a single tube
JP7612678B2 (ja) 2019-10-01 2025-01-14 ウニベルシテテット イ トロムソ-ノルゲス アークティスク ウニベルシテット 海洋性dnaポリメラーゼi
CN114729341A (zh) 2019-10-01 2022-07-08 特罗姆瑟大学-挪威北极圈大学 Dna聚合酶和dna聚合酶衍生的3′-5′外切核酸酶
WO2022040557A2 (en) * 2020-08-21 2022-02-24 Ultima Genomics, Inc. Compositions for surface amplification and uses thereof
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits
EP4414460A1 (en) 2023-02-07 2024-08-14 Arcticzymes AS Compositions comprising a sequence specific endoribonuclease and methods of use
WO2025168209A1 (en) 2024-02-07 2025-08-14 Arcticzymes As Compositions comprising a sequence specific endoribonuclease and methods of use

Family Cites Families (26)

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US5674679A (en) 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US5756285A (en) 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
CA2101119A1 (en) * 1992-08-17 1994-02-18 George T. Walker Exonuclease decontamination method
CA2137728C (en) 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
CA2168712A1 (en) 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
DE69627189T2 (de) * 1995-12-15 2004-02-05 Duke University Verfahren zum nachweis und zur entfernung von mutantsequenzen, die während enzymatischer amplifikation entstehen
GB9918150D0 (en) 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
US6379940B2 (en) 2000-03-21 2002-04-30 Usb Corporation Stable composition comprising a nuclease and a phosphatase
US8361712B2 (en) 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
US20030087278A1 (en) 2001-08-15 2003-05-08 Jon Sayers Nuclease assay
DK1444354T3 (da) 2001-11-13 2007-03-19 Serono Genetics Inst Sa Fremgangsmåde til forbedring af effektiviteten af en enzymatisk oprensningsproces
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
US20040096870A1 (en) 2002-06-28 2004-05-20 Sention, Inc. Methods of detecting sequence differences
JP3959315B2 (ja) 2002-07-26 2007-08-15 株式会社東芝 核酸汚染防止方法、それにおいて使用される抱埋化プライマーおよびそれを具備するキット
US20040126765A1 (en) 2002-12-27 2004-07-01 Adams Craig W. Method and compositions for sequencing nucleic acid molecules
AU2004277589A1 (en) 2003-09-30 2005-04-14 Perkinelmer Las, Inc. Compositions and processes for genotyping single nucleotide polymorphisms
WO2006095769A1 (ja) 2005-03-08 2006-09-14 Takara Bio Inc. 低温性微生物由来エンドヌクレアーゼ
WO2007143206A2 (en) 2006-06-02 2007-12-13 Epicentre Technologies Compositions and methods for removal of dna from a sample
WO2009100080A1 (en) 2008-02-04 2009-08-13 Life Technologies Corporation Composition and method for sequencing nucleic acid
JP5455243B2 (ja) * 2008-06-18 2014-03-26 独立行政法人理化学研究所 未知の配列を含む増幅した二本鎖核酸の混合物を製造する方法
WO2010045326A1 (en) 2008-10-14 2010-04-22 Barnes Wayne M In vitro recombination methods using 5' exonuclease
CN102439043A (zh) 2009-01-30 2012-05-02 牛津纳米孔技术有限公司 杂交连接物
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
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GB201120494D0 (en) 2011-11-29 2012-01-11 Univ Sheffield Modified exonuclease
LT2814954T (lt) 2012-02-17 2017-05-10 Biotec Pharmacon Asa Endonukleazės

Also Published As

Publication number Publication date
JP2017523803A (ja) 2017-08-24
KR102432501B1 (ko) 2022-08-12
GB201414745D0 (en) 2014-10-01
PL3183340T3 (pl) 2019-12-31
JP6316505B2 (ja) 2018-04-25
EP3183340B1 (en) 2019-05-15
US10787702B2 (en) 2020-09-29
CN107109383A (zh) 2017-08-29
US20180363042A1 (en) 2018-12-20
US20200332352A9 (en) 2020-10-22
US20200071753A1 (en) 2020-03-05
US10415082B2 (en) 2019-09-17
WO2016026574A1 (en) 2016-02-25
CN107109383B (zh) 2019-04-02
EP3183340A1 (en) 2017-06-28
US20170233800A1 (en) 2017-08-17
KR20180040736A (ko) 2018-04-20
LT3183340T (lt) 2019-10-25
CA2956853A1 (en) 2016-02-25
CA2956853C (en) 2022-05-17
US10087483B2 (en) 2018-10-02
KR20170040263A (ko) 2017-04-12
ES2742186T3 (es) 2020-02-13

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