DK1141335T3 - Kemisk modificerede enzymer med multipelt ladede varianter - Google Patents
Kemisk modificerede enzymer med multipelt ladede varianterInfo
- Publication number
- DK1141335T3 DK1141335T3 DK99966472T DK99966472T DK1141335T3 DK 1141335 T3 DK1141335 T3 DK 1141335T3 DK 99966472 T DK99966472 T DK 99966472T DK 99966472 T DK99966472 T DK 99966472T DK 1141335 T3 DK1141335 T3 DK 1141335T3
- Authority
- DK
- Denmark
- Prior art keywords
- chemically modified
- modified enzymes
- multiple charged
- cysteine residues
- enzymes
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 abstract 3
- 125000003396 thiol group Chemical class [H]S* 0.000 abstract 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 125000001424 substituent group Chemical group 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
- C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
- C12N9/6408—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/33—Chemical structure of the base
- C12N2310/334—Modified C
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11313098P | 1998-12-21 | 1998-12-21 | |
| PCT/US1999/030362 WO2000037658A2 (en) | 1998-12-21 | 1999-12-20 | Chemically modified enzymes with multiple charged variants |
| US09/467,536 US6379942B1 (en) | 1998-12-21 | 1999-12-20 | Chemically modified enzymes with multiple charged variants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1141335T3 true DK1141335T3 (da) | 2009-11-09 |
Family
ID=26810726
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK99966472T DK1141335T3 (da) | 1998-12-21 | 1999-12-20 | Kemisk modificerede enzymer med multipelt ladede varianter |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US6379942B1 (da) |
| EP (1) | EP1141335B1 (da) |
| JP (1) | JP2002533087A (da) |
| AT (1) | ATE438724T1 (da) |
| AU (1) | AU2200100A (da) |
| CA (1) | CA2353789C (da) |
| DE (1) | DE69941223D1 (da) |
| DK (1) | DK1141335T3 (da) |
| WO (1) | WO2000037658A2 (da) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| ES2368718T3 (es) * | 1997-10-23 | 2011-11-21 | Danisco Us Inc. | Variantes de subtilisina con múltiples sustituciones. |
| DK1042501T3 (da) * | 1997-12-24 | 2007-08-13 | Genencor Int | Fremgangsmåde til analyse af vaskeevnen af et enzym |
| US6569663B1 (en) | 1998-03-26 | 2003-05-27 | The Procter & Gamble Company | Serine protease variants having amino acid substitutions |
| US6495136B1 (en) | 1998-03-26 | 2002-12-17 | The Procter & Gamble Company | Proteases having modified amino acid sequences conjugated to addition moieties |
| US6908757B1 (en) | 1998-03-26 | 2005-06-21 | The Procter & Gamble Company | Serine protease variants having amino acid deletions and substitutions |
| WO2000028007A2 (en) * | 1998-11-10 | 2000-05-18 | Genencor International, Inc. | Chemically modified mutant serine hydrolases |
| JP5180417B2 (ja) * | 1999-04-28 | 2013-04-10 | ジェネンコー・インターナショナル・インク | 特異的に標的化された触媒性アンタゴニストおよびその使用 |
| CA2379712A1 (en) | 1999-07-22 | 2001-02-01 | The Procter & Gamble Company | Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions |
| BR0012692A (pt) * | 1999-07-22 | 2002-04-09 | Procter & Gamble | Conjugado de protease, composição de limpeza e composição de tratamento pessoal |
| CZ2002212A3 (cs) | 1999-07-22 | 2002-07-17 | The Procter & Gamble Company | Varianty subtilisinové proteasy mající aminokyselinové substituce v definovaných epitopových oblastech |
| MXPA02000842A (es) | 1999-07-22 | 2002-07-30 | Procter & Gamble | Conjugados de proteasa que tienen sitios de corte protegidos estericamente. |
| US6946128B1 (en) | 1999-07-22 | 2005-09-20 | The Procter & Gamble Company | Protease conjugates having sterically protected epitope regions |
| US6727085B2 (en) | 1999-12-15 | 2004-04-27 | Fanoe Tina Sejersgaard | Subtilase variants having an improved wash performance on egg stains |
| CN101974375B (zh) * | 1999-12-15 | 2014-07-02 | 诺沃奇梅兹有限公司 | 对蛋渍具有改进洗涤性能的枯草杆菌酶变体 |
| AU758744B2 (en) * | 2000-10-31 | 2003-03-27 | F. Hoffmann-La Roche Ag | Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain |
| US20040097720A1 (en) * | 2001-04-02 | 2004-05-20 | Bott Richard R. | Use of glycodendrimer reagents for inhibiting adhesion by microorganisms |
| DE10206620B4 (de) * | 2002-02-15 | 2005-08-18 | Henkel Kgaa | Vorrichtung und Verfahren zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf Flächengebilde sowie Verwendung der Vorrichtung |
| KR100475133B1 (ko) * | 2002-09-13 | 2005-03-10 | 한국생명공학연구원 | 효모 표면 발현 벡터를 이용하여 변이 리파제를스크리닝하는 방법 및 신규 변이 리파제 |
| US7888093B2 (en) | 2002-11-06 | 2011-02-15 | Novozymes A/S | Subtilase variants |
| TWI319007B (en) * | 2002-11-06 | 2010-01-01 | Novozymes As | Subtilase variants |
| EP1734991A4 (en) | 2004-04-14 | 2012-10-24 | Avirid Inc | COMPOSITIONS COMPRISING MODIFIED NUCLEASES DIRECTED AGAINST VIRAL NUCLEIC ACIDS AND METHODS OF USE FOR THE PREVENTION AND TREATMENT OF VIRAL DISEASES |
| WO2006063587A1 (en) * | 2004-12-14 | 2006-06-22 | Novozymes A/S | Testing the activity of an enzyme using absorbance |
| WO2008094222A2 (en) * | 2006-10-06 | 2008-08-07 | Trustees Of Princeton | Porphyrin catalysts and methods of use thereof |
| EP2370451B1 (en) | 2008-12-02 | 2016-11-16 | Wave Life Sciences Japan, Inc. | Method for the synthesis of phosphorus atom modified nucleic acids |
| KR101885383B1 (ko) | 2009-07-06 | 2018-08-03 | 웨이브 라이프 사이언시스 리미티드 | 신규한 핵산 프로드러그 및 그의 사용 방법 |
| US8765432B2 (en) | 2009-12-18 | 2014-07-01 | Oligasis, Llc | Targeted drug phosphorylcholine polymer conjugates |
| EP3549963B1 (en) | 2010-04-15 | 2022-01-12 | Kodiak Sciences Inc. | High molecular weight zwitterion-containing polymers |
| US10428019B2 (en) | 2010-09-24 | 2019-10-01 | Wave Life Sciences Ltd. | Chiral auxiliaries |
| EP2734208B1 (en) | 2011-07-19 | 2017-03-01 | Wave Life Sciences Ltd. | Methods for the synthesis of functionalized nucleic acids |
| MX2014008073A (es) | 2011-12-30 | 2014-10-06 | Butamax Advanced Biofuels Llc | Cambios geneticos para la produccion de butanol. |
| CN104661664B (zh) | 2012-07-13 | 2020-07-03 | 波涛生命科学有限公司 | 手性控制 |
| CA2879066C (en) | 2012-07-13 | 2019-08-13 | Shin Nippon Biomedical Laboratories, Ltd. | Chiral nucleic acid adjuvant |
| PL2872485T3 (pl) | 2012-07-13 | 2021-05-31 | Wave Life Sciences Ltd. | Asymetryczna grupa pomocnicza |
| JPWO2015108046A1 (ja) | 2014-01-15 | 2017-03-23 | 株式会社新日本科学 | 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤 |
| WO2015108047A1 (ja) | 2014-01-15 | 2015-07-23 | 株式会社新日本科学 | 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤 |
| JPWO2015108048A1 (ja) | 2014-01-15 | 2017-03-23 | 株式会社新日本科学 | 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤 |
| CN114786731A (zh) | 2019-10-10 | 2022-07-22 | 科达制药股份有限公司 | 治疗眼部病症的方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5244791A (en) | 1984-05-29 | 1993-09-14 | Genecor International, Inc. | Methods of ester hydrolysis |
| US5155033A (en) | 1989-01-06 | 1992-10-13 | Genencor, Inc. | Subtilisins modified at position 225 resulting in a shift in catalytic activity |
| US5260207A (en) * | 1987-04-06 | 1993-11-09 | Enzon Labs Inc. | Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins |
| CN1056187C (zh) | 1988-02-11 | 2000-09-06 | 金克克国际有限公司 | 新的蛋白水解酶及其在洗涤剂中的应用 |
| DK97190D0 (da) | 1990-04-19 | 1990-04-19 | Novo Nordisk As | Oxidationsstabile detergentenzymer |
| CA2087342C (en) | 1990-08-09 | 2003-12-23 | Lars Abrahmsen | Serine protease variants having peptide ligase activity |
| US5629173A (en) | 1990-08-09 | 1997-05-13 | Genentech, Inc. | Methods of use of serine protease variants having peptide ligase activity |
| US5316935A (en) | 1992-04-06 | 1994-05-31 | California Institute Of Technology | Subtilisin variants suitable for hydrolysis and synthesis in organic media |
| MA23346A1 (fr) | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| US5837516A (en) | 1995-03-03 | 1998-11-17 | Genentech, Inc. | Subtilisin variants capable of cleaving substrates containing basic residues |
| US5837517A (en) | 1995-05-05 | 1998-11-17 | Novo Nordisk A/S | Protease variants and compositions |
| US6946280B1 (en) | 1996-03-29 | 2005-09-20 | Genencor International, Inc. | Enzyme multimer and process of producing same |
| CN1246146A (zh) | 1996-11-26 | 2000-03-01 | 金克克国际有限公司 | 化学修饰的酶 |
-
1999
- 1999-12-20 DK DK99966472T patent/DK1141335T3/da active
- 1999-12-20 DE DE69941223T patent/DE69941223D1/de not_active Expired - Lifetime
- 1999-12-20 AT AT99966472T patent/ATE438724T1/de not_active IP Right Cessation
- 1999-12-20 EP EP99966472A patent/EP1141335B1/en not_active Expired - Lifetime
- 1999-12-20 AU AU22001/00A patent/AU2200100A/en not_active Abandoned
- 1999-12-20 WO PCT/US1999/030362 patent/WO2000037658A2/en not_active Ceased
- 1999-12-20 CA CA2353789A patent/CA2353789C/en not_active Expired - Fee Related
- 1999-12-20 US US09/467,536 patent/US6379942B1/en not_active Expired - Lifetime
- 1999-12-20 JP JP2000589712A patent/JP2002533087A/ja active Pending
-
2002
- 2002-02-12 US US10/075,907 patent/US20020127695A1/en not_active Abandoned
-
2004
- 2004-11-19 US US10/993,827 patent/US7371553B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU2200100A (en) | 2000-07-12 |
| JP2002533087A (ja) | 2002-10-08 |
| US7371553B2 (en) | 2008-05-13 |
| US6379942B1 (en) | 2002-04-30 |
| DE69941223D1 (de) | 2009-09-17 |
| US20050089966A1 (en) | 2005-04-28 |
| EP1141335A2 (en) | 2001-10-10 |
| CA2353789A1 (en) | 2000-06-29 |
| WO2000037658A3 (en) | 2000-11-09 |
| US20020127695A1 (en) | 2002-09-12 |
| EP1141335B1 (en) | 2009-08-05 |
| ATE438724T1 (de) | 2009-08-15 |
| CA2353789C (en) | 2012-02-14 |
| WO2000037658A2 (en) | 2000-06-29 |
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