DE69941223D1 - Chemisch modifizierte enzymen mit mehrfachgeladenen varianten - Google Patents
Chemisch modifizierte enzymen mit mehrfachgeladenen variantenInfo
- Publication number
- DE69941223D1 DE69941223D1 DE69941223T DE69941223T DE69941223D1 DE 69941223 D1 DE69941223 D1 DE 69941223D1 DE 69941223 T DE69941223 T DE 69941223T DE 69941223 T DE69941223 T DE 69941223T DE 69941223 D1 DE69941223 D1 DE 69941223D1
- Authority
- DE
- Germany
- Prior art keywords
- variations
- chemically modified
- modified enzymes
- multiple charged
- cysteine residues
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 abstract 3
- 125000003396 thiol group Chemical class [H]S* 0.000 abstract 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 125000001424 substituent group Chemical group 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
- C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
- C12N9/6408—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/33—Chemical structure of the base
- C12N2310/334—Modified C
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11313098P | 1998-12-21 | 1998-12-21 | |
| PCT/US1999/030362 WO2000037658A2 (en) | 1998-12-21 | 1999-12-20 | Chemically modified enzymes with multiple charged variants |
| US09/467,536 US6379942B1 (en) | 1998-12-21 | 1999-12-20 | Chemically modified enzymes with multiple charged variants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE69941223D1 true DE69941223D1 (de) | 2009-09-17 |
Family
ID=26810726
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69941223T Expired - Lifetime DE69941223D1 (de) | 1998-12-21 | 1999-12-20 | Chemisch modifizierte enzymen mit mehrfachgeladenen varianten |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US6379942B1 (de) |
| EP (1) | EP1141335B1 (de) |
| JP (1) | JP2002533087A (de) |
| AT (1) | ATE438724T1 (de) |
| AU (1) | AU2200100A (de) |
| CA (1) | CA2353789C (de) |
| DE (1) | DE69941223D1 (de) |
| DK (1) | DK1141335T3 (de) |
| WO (1) | WO2000037658A2 (de) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| ES2367505T3 (es) * | 1997-10-23 | 2011-11-04 | Danisco Us Inc. | Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes. |
| DK1042501T3 (da) * | 1997-12-24 | 2007-08-13 | Genencor Int | Fremgangsmåde til analyse af vaskeevnen af et enzym |
| US6495136B1 (en) | 1998-03-26 | 2002-12-17 | The Procter & Gamble Company | Proteases having modified amino acid sequences conjugated to addition moieties |
| US6908757B1 (en) | 1998-03-26 | 2005-06-21 | The Procter & Gamble Company | Serine protease variants having amino acid deletions and substitutions |
| WO1999049056A1 (en) | 1998-03-26 | 1999-09-30 | The Procter & Gamble Company | Serine protease variants having amino acid substitutions |
| DE69940978D1 (de) * | 1998-11-10 | 2009-07-23 | Univ Toronto | Chemisch modifizierte mutante serinhydrolasen |
| JP5180417B2 (ja) * | 1999-04-28 | 2013-04-10 | ジェネンコー・インターナショナル・インク | 特異的に標的化された触媒性アンタゴニストおよびその使用 |
| CZ2002211A3 (cs) * | 1999-07-22 | 2002-05-15 | The Procter & Gamble Company | Proteázové konjugáty se stericky chráněnými oblastmi epitopu |
| JP2003505070A (ja) | 1999-07-22 | 2003-02-12 | ザ、プロクター、エンド、ギャンブル、カンパニー | 定められたエピトープ領域においてアミノ酸置換を有するサブチリシンプロテアーゼ変異体 |
| CZ2002171A3 (cs) * | 1999-07-22 | 2002-06-12 | The Procter & Gamble Company | Proteinázový konjugát, čistící prostředek a prostředek osobní péče |
| CZ2002220A3 (cs) | 1999-07-22 | 2002-05-15 | The Procter & Gamble Company | Varianty subtilisinové proteasy s delecemi a substitucemi aminokyselin v definovaných epitopových regionech |
| US6946128B1 (en) | 1999-07-22 | 2005-09-20 | The Procter & Gamble Company | Protease conjugates having sterically protected epitope regions |
| US6727085B2 (en) | 1999-12-15 | 2004-04-27 | Fanoe Tina Sejersgaard | Subtilase variants having an improved wash performance on egg stains |
| EP1244779B1 (de) * | 1999-12-15 | 2014-05-07 | Novozymes A/S | Subtilasevarianten mit verbesserter waschleistung bei eierflecken |
| AU758744B2 (en) * | 2000-10-31 | 2003-03-27 | F. Hoffmann-La Roche Ag | Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain |
| US20040097720A1 (en) * | 2001-04-02 | 2004-05-20 | Bott Richard R. | Use of glycodendrimer reagents for inhibiting adhesion by microorganisms |
| DE10206620B4 (de) * | 2002-02-15 | 2005-08-18 | Henkel Kgaa | Vorrichtung und Verfahren zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf Flächengebilde sowie Verwendung der Vorrichtung |
| KR100475133B1 (ko) * | 2002-09-13 | 2005-03-10 | 한국생명공학연구원 | 효모 표면 발현 벡터를 이용하여 변이 리파제를스크리닝하는 방법 및 신규 변이 리파제 |
| US7888093B2 (en) | 2002-11-06 | 2011-02-15 | Novozymes A/S | Subtilase variants |
| TWI319007B (en) * | 2002-11-06 | 2010-01-01 | Novozymes As | Subtilase variants |
| US9592277B2 (en) | 2004-04-14 | 2017-03-14 | Avirid, Inc. | Compositions with modified nucleases targeted to viral nucleic acids and methods of use for prevention and treatment of viral diseases |
| WO2006063587A1 (en) * | 2004-12-14 | 2006-06-22 | Novozymes A/S | Testing the activity of an enzyme using absorbance |
| WO2008094222A2 (en) * | 2006-10-06 | 2008-08-07 | Trustees Of Princeton | Porphyrin catalysts and methods of use thereof |
| CN102282155B (zh) | 2008-12-02 | 2017-06-09 | 日本波涛生命科学公司 | 磷原子修饰的核酸的合成方法 |
| CA2767253A1 (en) | 2009-07-06 | 2011-01-13 | Ontorii, Inc. | Novel nucleic acid prodrugs and methods of use thereof |
| US8765432B2 (en) | 2009-12-18 | 2014-07-01 | Oligasis, Llc | Targeted drug phosphorylcholine polymer conjugates |
| MX365521B (es) | 2010-04-15 | 2019-06-06 | Oligasis Star | Polimeros que contienen zwiteriones de alto peso molecular. |
| DK2620428T3 (da) | 2010-09-24 | 2019-07-01 | Wave Life Sciences Ltd | Asymmetrisk hjælpegruppe |
| JP6128529B2 (ja) | 2011-07-19 | 2017-05-17 | ウェイブ ライフ サイエンシズ リミテッドＷａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｌｔｄ． | 官能化核酸の合成のための方法 |
| MX2014008073A (es) | 2011-12-30 | 2014-10-06 | Butamax Advanced Biofuels Llc | Cambios geneticos para la produccion de butanol. |
| CN112007045A (zh) | 2012-07-13 | 2020-12-01 | 波涛生命科学有限公司 | 手性控制 |
| KR101850319B1 (ko) | 2012-07-13 | 2018-04-20 | 웨이브 라이프 사이언시스 리미티드 | 비대칭 보조 그룹 |
| SG11201500243WA (en) | 2012-07-13 | 2015-04-29 | Shin Nippon Biomedical Lab Ltd | Chiral nucleic acid adjuvant |
| JPWO2015108046A1 (ja) | 2014-01-15 | 2017-03-23 | 株式会社新日本科学 | 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤 |
| WO2015108048A1 (ja) | 2014-01-15 | 2015-07-23 | 株式会社新日本科学 | 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤 |
| EP3095461A4 (de) | 2014-01-15 | 2017-08-23 | Shin Nippon Biomedical Laboratories, Ltd. | Chirales nukleinsäure-adjuvans mit immunitätsinduktionswirkung und immunitätsinduktionsaktivator |
| WO2021072265A1 (en) | 2019-10-10 | 2021-04-15 | Kodiak Sciences Inc. | Methods of treating an eye disorder |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5244791A (en) | 1984-05-29 | 1993-09-14 | Genecor International, Inc. | Methods of ester hydrolysis |
| US5155033A (en) | 1989-01-06 | 1992-10-13 | Genencor, Inc. | Subtilisins modified at position 225 resulting in a shift in catalytic activity |
| US5260207A (en) * | 1987-04-06 | 1993-11-09 | Enzon Labs Inc. | Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins |
| PT89702B (pt) | 1988-02-11 | 1994-04-29 | Gist Brocades Nv | Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem |
| DK97190D0 (da) | 1990-04-19 | 1990-04-19 | Novo Nordisk As | Oxidationsstabile detergentenzymer |
| US5629173A (en) | 1990-08-09 | 1997-05-13 | Genentech, Inc. | Methods of use of serine protease variants having peptide ligase activity |
| DE69131834T2 (de) | 1990-08-09 | 2000-05-31 | Genentech, Inc. | Serinproteasevarianten mit peptidligase aktivität |
| US5316935A (en) | 1992-04-06 | 1994-05-31 | California Institute Of Technology | Subtilisin variants suitable for hydrolysis and synthesis in organic media |
| MA23346A1 (fr) | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| US5837516A (en) | 1995-03-03 | 1998-11-17 | Genentech, Inc. | Subtilisin variants capable of cleaving substrates containing basic residues |
| US5837517A (en) | 1995-05-05 | 1998-11-17 | Novo Nordisk A/S | Protease variants and compositions |
| US6946280B1 (en) | 1996-03-29 | 2005-09-20 | Genencor International, Inc. | Enzyme multimer and process of producing same |
| NZ336434A (en) * | 1996-11-26 | 2001-03-30 | Genencor Int | Modified subtilisin enzymes with thiol side chains at cysteine position 62, 217, 156 or 166 |
-
1999
- 1999-12-20 EP EP99966472A patent/EP1141335B1/de not_active Expired - Lifetime
- 1999-12-20 CA CA2353789A patent/CA2353789C/en not_active Expired - Fee Related
- 1999-12-20 AU AU22001/00A patent/AU2200100A/en not_active Abandoned
- 1999-12-20 JP JP2000589712A patent/JP2002533087A/ja active Pending
- 1999-12-20 WO PCT/US1999/030362 patent/WO2000037658A2/en active Application Filing
- 1999-12-20 AT AT99966472T patent/ATE438724T1/de not_active IP Right Cessation
- 1999-12-20 DK DK99966472T patent/DK1141335T3/da active
- 1999-12-20 DE DE69941223T patent/DE69941223D1/de not_active Expired - Lifetime
- 1999-12-20 US US09/467,536 patent/US6379942B1/en not_active Expired - Lifetime
-
2002
- 2002-02-12 US US10/075,907 patent/US20020127695A1/en not_active Abandoned
-
2004
- 2004-11-19 US US10/993,827 patent/US7371553B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20050089966A1 (en) | 2005-04-28 |
| ATE438724T1 (de) | 2009-08-15 |
| JP2002533087A (ja) | 2002-10-08 |
| DK1141335T3 (da) | 2009-11-09 |
| CA2353789C (en) | 2012-02-14 |
| WO2000037658A3 (en) | 2000-11-09 |
| EP1141335B1 (de) | 2009-08-05 |
| US6379942B1 (en) | 2002-04-30 |
| US7371553B2 (en) | 2008-05-13 |
| CA2353789A1 (en) | 2000-06-29 |
| WO2000037658A2 (en) | 2000-06-29 |
| US20020127695A1 (en) | 2002-09-12 |
| EP1141335A2 (de) | 2001-10-10 |
| AU2200100A (en) | 2000-07-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |