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DK0699768T3 - Fremgangsmåder til quenching i opløsning af fluorescens-mærkede oligonnukleotidprober - Google Patents

Fremgangsmåder til quenching i opløsning af fluorescens-mærkede oligonnukleotidprober

Info

Publication number
DK0699768T3
DK0699768T3 DK95113012T DK95113012T DK0699768T3 DK 0699768 T3 DK0699768 T3 DK 0699768T3 DK 95113012 T DK95113012 T DK 95113012T DK 95113012 T DK95113012 T DK 95113012T DK 0699768 T3 DK0699768 T3 DK 0699768T3
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DK
Denmark
Prior art keywords
quenching
fluorescence
methods
solution
oligonucleotide probes
Prior art date
Application number
DK95113012T
Other languages
English (en)
Inventor
Mary Ellen Fisher
Robert Malcolm Watson
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
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Publication of DK0699768T3 publication Critical patent/DK0699768T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
DK95113012T 1994-09-01 1995-08-18 Fremgangsmåder til quenching i opløsning af fluorescens-mærkede oligonnukleotidprober DK0699768T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/299,682 US5491063A (en) 1994-09-01 1994-09-01 Methods for in-solution quenching of fluorescently labeled oligonucleotide probes

Publications (1)

Publication Number Publication Date
DK0699768T3 true DK0699768T3 (da) 2003-10-20

Family

ID=23155803

Family Applications (1)

Application Number Title Priority Date Filing Date
DK95113012T DK0699768T3 (da) 1994-09-01 1995-08-18 Fremgangsmåder til quenching i opløsning af fluorescens-mærkede oligonnukleotidprober

Country Status (9)

Country Link
US (1) US5491063A (da)
EP (1) EP0699768B1 (da)
JP (1) JP3795557B2 (da)
AT (1) ATE243759T1 (da)
CA (1) CA2157200C (da)
DE (1) DE69531133T2 (da)
DK (1) DK0699768T3 (da)
ES (1) ES2202333T3 (da)
PT (1) PT699768E (da)

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ATE243759T1 (de) 2003-07-15
US5491063A (en) 1996-02-13
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