DE69924118D1 - Verfahren und reagentiensatz zur erkennung von antiviralen wirkstoff resistenzen - Google Patents
Verfahren und reagentiensatz zur erkennung von antiviralen wirkstoff resistenzenInfo
- Publication number
- DE69924118D1 DE69924118D1 DE69924118T DE69924118T DE69924118D1 DE 69924118 D1 DE69924118 D1 DE 69924118D1 DE 69924118 T DE69924118 T DE 69924118T DE 69924118 T DE69924118 T DE 69924118T DE 69924118 D1 DE69924118 D1 DE 69924118D1
- Authority
- DE
- Germany
- Prior art keywords
- reverse transcriptase
- dna
- drug
- assay
- amplified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 2
- 230000000840 anti-viral effect Effects 0.000 title 1
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 239000003814 drug Substances 0.000 abstract 5
- 229940079593 drug Drugs 0.000 abstract 5
- 108020004414 DNA Proteins 0.000 abstract 4
- 102100034343 Integrase Human genes 0.000 abstract 4
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 4
- 238000003556 assay Methods 0.000 abstract 3
- 108020004635 Complementary DNA Proteins 0.000 abstract 2
- 108020001019 DNA Primers Proteins 0.000 abstract 2
- 239000003155 DNA primer Substances 0.000 abstract 2
- 241000710188 Encephalomyocarditis virus Species 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 2
- 238000010804 cDNA synthesis Methods 0.000 abstract 2
- 239000002299 complementary DNA Substances 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 239000013615 primer Substances 0.000 abstract 2
- 239000003419 rna directed dna polymerase inhibitor Substances 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 1
- 239000012807 PCR reagent Substances 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000001177 retroviral effect Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 241001430294 unidentified retrovirus Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9005198P | 1998-06-19 | 1998-06-19 | |
| US90051P | 1998-06-19 | ||
| PCT/US1999/013957 WO1999066068A2 (en) | 1998-06-19 | 1999-06-18 | Method and kit for detecting resistance to antiviral drugs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69924118D1 true DE69924118D1 (de) | 2005-04-14 |
| DE69924118T2 DE69924118T2 (de) | 2005-12-22 |
Family
ID=22221041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69924118T Expired - Lifetime DE69924118T2 (de) | 1998-06-19 | 1999-06-18 | Verfahren und reagentiensatz zur erkennung von antiviralen wirkstoff resistenzen |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1100959B1 (de) |
| AT (1) | ATE290605T1 (de) |
| CA (1) | CA2331260C (de) |
| DE (1) | DE69924118T2 (de) |
| ES (1) | ES2237923T3 (de) |
| WO (1) | WO1999066068A2 (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1160332A1 (de) * | 2000-05-30 | 2001-12-05 | Amsterdam Support Diagnostics B.V. | Verfahren zum Nachweis von enzymatischer Aktivität oder von Resistenz gegen enzymatiche Inhibitoren |
| US6664065B2 (en) * | 2000-06-21 | 2003-12-16 | Robert B. Harris | Measuring viral reverse transcriptase activity |
| CN110832083A (zh) * | 2017-07-04 | 2020-02-21 | 卡维迪有限公司 | 用于通过测量酶活性来评估病毒对治疗的敏感性的方法及其系统 |
| CN116732242A (zh) * | 2023-03-16 | 2023-09-12 | 赛立安生物技术(广州)有限公司 | 一种用于检测hiv-1蛋白酶区和逆转录酶区耐药突变的引物组、试剂盒及其检测方法 |
| US11977085B1 (en) | 2023-09-05 | 2024-05-07 | Elan Ehrlich | Date rape drug detection device and method of using same |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU4248593A (en) * | 1992-05-14 | 1993-12-13 | Mark Holodniy | Polymerase chain reaction assays for monitoring antiviral therapy |
| EP0805873B1 (de) * | 1995-01-27 | 1999-09-01 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by The Secretary, Department of Health and Human Services | Verfahren zum sensitiven nachweis von reverser transcriptase |
| EP1170380B1 (de) * | 1996-01-29 | 2009-11-04 | Monogram Biosciences, Inc. | Verfahren zur Bestimmung antiviraler Wirkstoff Suszeptibilität und Resistenz sowie antivirales Wirkstoff-Screening |
-
1999
- 1999-06-18 AT AT99930490T patent/ATE290605T1/de not_active IP Right Cessation
- 1999-06-18 ES ES99930490T patent/ES2237923T3/es not_active Expired - Lifetime
- 1999-06-18 EP EP99930490A patent/EP1100959B1/de not_active Expired - Lifetime
- 1999-06-18 DE DE69924118T patent/DE69924118T2/de not_active Expired - Lifetime
- 1999-06-18 WO PCT/US1999/013957 patent/WO1999066068A2/en active IP Right Grant
- 1999-06-18 CA CA002331260A patent/CA2331260C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999066068A3 (en) | 2001-03-29 |
| DE69924118T2 (de) | 2005-12-22 |
| ATE290605T1 (de) | 2005-03-15 |
| EP1100959B1 (de) | 2005-03-09 |
| CA2331260A1 (en) | 1999-12-23 |
| WO1999066068A2 (en) | 1999-12-23 |
| ES2237923T3 (es) | 2005-08-01 |
| CA2331260C (en) | 2008-03-18 |
| EP1100959A2 (de) | 2001-05-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |