DE69729085T2 - Gesteigerte Expression proteolytischer Enzyme in ... - Google Patents
Gesteigerte Expression proteolytischer Enzyme in ... Download PDFInfo
- Publication number
- DE69729085T2 DE69729085T2 DE69729085T DE69729085T DE69729085T2 DE 69729085 T2 DE69729085 T2 DE 69729085T2 DE 69729085 T DE69729085 T DE 69729085T DE 69729085 T DE69729085 T DE 69729085T DE 69729085 T2 DE69729085 T2 DE 69729085T2
- Authority
- DE
- Germany
- Prior art keywords
- provides
- amino
- koji mold
- enzymes
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000035195 Peptidases Human genes 0.000 title abstract 2
- 108091005804 Peptidases Proteins 0.000 title abstract 2
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 title abstract 2
- 240000006439 Aspergillus oryzae Species 0.000 abstract 6
- 235000002247 Aspergillus oryzae Nutrition 0.000 abstract 6
- 150000001413 amino acids Chemical group 0.000 abstract 3
- 230000000694 effects Effects 0.000 abstract 3
- 102000052510 DNA-Binding Proteins Human genes 0.000 abstract 2
- 101710096438 DNA-binding protein Proteins 0.000 abstract 2
- 102000005593 Endopeptidases Human genes 0.000 abstract 2
- 108010059378 Endopeptidases Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 229940088598 enzyme Drugs 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 2
- 235000018102 proteins Nutrition 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 102000018389 Exopeptidases Human genes 0.000 abstract 1
- 108010091443 Exopeptidases Proteins 0.000 abstract 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 abstract 1
- 229930182816 L-glutamine Natural products 0.000 abstract 1
- 102000002704 Leucyl aminopeptidase Human genes 0.000 abstract 1
- 108010004098 Leucyl aminopeptidase Proteins 0.000 abstract 1
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 1
- 108010073771 Soybean Proteins Proteins 0.000 abstract 1
- 101150044844 areA gene Proteins 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 abstract 1
- 239000003531 protein hydrolysate Substances 0.000 abstract 1
- 235000019710 soybean protein Nutrition 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97111378A EP0897003B1 (de) | 1997-07-05 | 1997-07-05 | Verstärkte Expression von proteolytischen Enzymen in Koji Pilz |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69729085D1 DE69729085D1 (de) | 2004-06-17 |
| DE69729085T2 true DE69729085T2 (de) | 2005-04-21 |
Family
ID=8227021
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69729085T Expired - Fee Related DE69729085T2 (de) | 1997-07-05 | 1997-07-05 | Gesteigerte Expression proteolytischer Enzyme in ... |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US6090607A (de) |
| EP (1) | EP0897003B1 (de) |
| JP (1) | JP2001500022A (de) |
| KR (1) | KR20000068489A (de) |
| CN (1) | CN1292068C (de) |
| AT (1) | ATE266727T1 (de) |
| AU (1) | AU753666B2 (de) |
| BR (1) | BR9806108A (de) |
| CA (1) | CA2263947A1 (de) |
| DE (1) | DE69729085T2 (de) |
| ES (1) | ES2219714T3 (de) |
| NZ (1) | NZ334461A (de) |
| PT (1) | PT897003E (de) |
| WO (1) | WO1999002691A1 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010112918A (ko) | 1999-03-11 | 2001-12-22 | 뷜르 로망 엘. | 탄소원의 존재 하에서의 국균에서의 단백질 분해 효소의발현 |
| EP1132461A1 (de) * | 2000-03-09 | 2001-09-12 | Kikkoman Corporation | Ein vielfach transformierter Aspergillus (koji) Pilz und dessen Verwendung zur Herstellung eines Geschmackverstärkers |
| KR100602807B1 (ko) * | 2001-03-30 | 2006-07-20 | 아지노모토 가부시키가이샤 | 단백질의 분비 생산 방법 |
| JP4029927B2 (ja) * | 2002-02-21 | 2008-01-09 | 財団法人野田産業科学研究所 | 転写因子、転写因子遺伝子、転写因子遺伝子を含む組み換えベクター、該ベクターによって形質転換された麹菌及び麹菌の使用法 |
| US20080305522A1 (en) * | 2004-01-28 | 2008-12-11 | Keiko Abe | Novel Taste-Modifying Polypeptide Nas, Dna Thereof and Use Thereof |
| JP4628123B2 (ja) * | 2005-02-04 | 2011-02-09 | 宝酒造株式会社 | 新規麹菌及びその用途 |
| JP4701440B2 (ja) | 2005-09-26 | 2011-06-15 | 財団法人野田産業科学研究所 | 麹菌蛋白質加水分解酵素の分泌を増大する組換えベクター |
| JP4469014B1 (ja) * | 2009-04-17 | 2010-05-26 | キッコーマン株式会社 | 大規模ゲノム重複を保持する麹菌 |
| CN102550895A (zh) * | 2010-12-28 | 2012-07-11 | 雀巢公司 | 来自于食物固态发酵的酶制剂 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55144884A (en) * | 1979-04-27 | 1980-11-12 | Kikkoman Corp | Preparation of koji |
| CH679544A5 (de) * | 1989-09-12 | 1992-03-13 | Nestle Sa | |
| CH679542A5 (de) * | 1989-11-27 | 1992-03-13 | Nestle Sa | |
| JPH07115969A (ja) * | 1993-10-25 | 1995-05-09 | Asahi Chem Ind Co Ltd | 加水分解蛋白質の製造方法 |
| US6025185A (en) * | 1994-06-17 | 2000-02-15 | Novo Nordisk Als | Fungus wherein the areA gene has been modified and an areA gene from Aspergillus oryzae |
| ATE227518T1 (de) * | 1996-07-09 | 2002-11-15 | Nestle Sa | Herstellung eines gewürzes mit fleischgeschmack |
-
1997
- 1997-07-05 EP EP97111378A patent/EP0897003B1/de not_active Expired - Lifetime
- 1997-07-05 PT PT97111378T patent/PT897003E/pt unknown
- 1997-07-05 ES ES97111378T patent/ES2219714T3/es not_active Expired - Lifetime
- 1997-07-05 AT AT97111378T patent/ATE266727T1/de not_active IP Right Cessation
- 1997-07-05 DE DE69729085T patent/DE69729085T2/de not_active Expired - Fee Related
-
1998
- 1998-05-01 WO PCT/EP1998/002785 patent/WO1999002691A1/en not_active Application Discontinuation
- 1998-05-01 NZ NZ334461A patent/NZ334461A/xx unknown
- 1998-05-01 KR KR1019997001897A patent/KR20000068489A/ko not_active Withdrawn
- 1998-05-01 BR BR9806108-9A patent/BR9806108A/pt not_active IP Right Cessation
- 1998-05-01 CA CA002263947A patent/CA2263947A1/en not_active Abandoned
- 1998-05-01 CN CNB988012871A patent/CN1292068C/zh not_active Expired - Fee Related
- 1998-05-01 US US09/254,325 patent/US6090607A/en not_active Expired - Fee Related
- 1998-05-01 JP JP11508030A patent/JP2001500022A/ja active Pending
- 1998-05-01 AU AU80166/98A patent/AU753666B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU8016698A (en) | 1999-02-08 |
| PT897003E (pt) | 2004-09-30 |
| EP0897003A1 (de) | 1999-02-17 |
| WO1999002691A1 (en) | 1999-01-21 |
| NZ334461A (en) | 2000-08-25 |
| AU753666B2 (en) | 2002-10-24 |
| US6090607A (en) | 2000-07-18 |
| ATE266727T1 (de) | 2004-05-15 |
| CN1292068C (zh) | 2006-12-27 |
| JP2001500022A (ja) | 2001-01-09 |
| BR9806108A (pt) | 1999-08-31 |
| ES2219714T3 (es) | 2004-12-01 |
| DE69729085D1 (de) | 2004-06-17 |
| CN1237205A (zh) | 1999-12-01 |
| EP0897003B1 (de) | 2004-05-12 |
| KR20000068489A (ko) | 2000-11-25 |
| CA2263947A1 (en) | 1999-01-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8339 | Ceased/non-payment of the annual fee |