DE69636080D1 - Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren - Google Patents
Eine in Kaskaden verlaufende Vervielfältigungsreaktion von NukleinsäurenInfo
- Publication number
- DE69636080D1 DE69636080D1 DE69636080T DE69636080T DE69636080D1 DE 69636080 D1 DE69636080 D1 DE 69636080D1 DE 69636080 T DE69636080 T DE 69636080T DE 69636080 T DE69636080 T DE 69636080T DE 69636080 D1 DE69636080 D1 DE 69636080D1
- Authority
- DE
- Germany
- Prior art keywords
- oligonucleotide
- nucleic acids
- amplification reaction
- circular
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000006243 chemical reaction Methods 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 5
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108020004638 Circular DNA Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 238000006073 displacement reaction Methods 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
- 239000002777 nucleoside Substances 0.000 abstract 1
- -1 nucleoside triphosphates Chemical class 0.000 abstract 1
- 239000001226 triphosphate Substances 0.000 abstract 1
- 235000011178 triphosphate Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Ceramic Products (AREA)
- Steroid Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK137995 | 1995-12-05 | ||
| DK137995 | 1995-12-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69636080D1 true DE69636080D1 (de) | 2006-06-01 |
| DE69636080T2 DE69636080T2 (de) | 2006-11-30 |
Family
ID=8104224
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69636080T Expired - Lifetime DE69636080T2 (de) | 1995-12-05 | 1996-12-05 | Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren |
| DE69627698T Expired - Lifetime DE69627698T2 (de) | 1995-12-05 | 1996-12-05 | Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69627698T Expired - Lifetime DE69627698T2 (de) | 1995-12-05 | 1996-12-05 | Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6610481B2 (de) |
| EP (2) | EP1300466B1 (de) |
| AT (2) | ATE324464T1 (de) |
| AU (1) | AU704750B2 (de) |
| CA (1) | CA2239287C (de) |
| DE (2) | DE69636080T2 (de) |
| DK (2) | DK1300466T3 (de) |
| ES (1) | ES2262902T3 (de) |
| WO (1) | WO1997020948A1 (de) |
Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6077668A (en) * | 1993-04-15 | 2000-06-20 | University Of Rochester | Highly sensitive multimeric nucleic acid probes |
| US7135312B2 (en) | 1993-04-15 | 2006-11-14 | University Of Rochester | Circular DNA vectors for synthesis of RNA and DNA |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| AU714486B2 (en) * | 1995-11-21 | 2000-01-06 | Yale University | Unimolecular segment amplification and detection |
| US6255082B1 (en) | 1998-09-15 | 2001-07-03 | Yale University | Artificial long terminal repeat vectors |
| WO2000015779A2 (en) * | 1998-09-15 | 2000-03-23 | Yale University | Molecular cloning using rolling circle amplification |
| US6986990B1 (en) * | 1999-04-27 | 2006-01-17 | Genox Research, Inc. | Pollen allergy-related gene 513 |
| US6368801B1 (en) | 2000-04-12 | 2002-04-09 | Molecular Staging, Inc. | Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase |
| AU2001271722B2 (en) | 2000-06-30 | 2006-04-13 | Qiagen, Gmbh | Signal amplification with lollipop probes |
| GB0025144D0 (en) * | 2000-10-13 | 2000-11-29 | Medical Res Council | Concatenated nucleic acid sequences |
| US6573051B2 (en) | 2001-03-09 | 2003-06-03 | Molecular Staging, Inc. | Open circle probes with intramolecular stem structures |
| WO2003002761A1 (en) * | 2001-06-29 | 2003-01-09 | Novozymes A/S | Method of multimerization and site-directed mutagenesis |
| GB2378245A (en) | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| GB2382137A (en) | 2001-11-20 | 2003-05-21 | Mats Gullberg | Nucleic acid enrichment |
| US7553619B2 (en) | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
| US8394944B2 (en) * | 2002-09-20 | 2013-03-12 | Siemens Healthcare Diagnostics Inc. | Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation |
| DE60324188D1 (de) | 2002-11-21 | 2008-11-27 | Epict Technologies | Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen |
| US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| US6977153B2 (en) | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
| WO2006035701A1 (ja) * | 2004-09-27 | 2006-04-06 | The Furukawa Electric Co., Ltd. | 接続部材 |
| CA2590933A1 (en) * | 2004-12-11 | 2006-06-15 | Cytogenix, Inc. | Cell free biosynthesis of high-quality nucleic acid and uses thereof |
| GB0504774D0 (en) * | 2005-03-08 | 2005-04-13 | Lingvitae As | Method |
| ATE483032T1 (de) | 2005-04-12 | 2010-10-15 | Olink Ab | Verfahren zur herstellung von oligonukleotiden |
| US7989166B2 (en) | 2005-04-12 | 2011-08-02 | In Situ Rcp A/S | Circle probes and their use in the identification of biomolecules |
| CA2611743C (en) | 2005-06-15 | 2019-12-31 | Callida Genomics, Inc. | Nucleic acid analysis by forming and tracking aliquoted fragments of a target polynucleotide |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| WO2007044671A2 (en) | 2005-10-06 | 2007-04-19 | Lucigen Corporation | Thermostable viral polymerases and methods of use |
| US7960104B2 (en) | 2005-10-07 | 2011-06-14 | Callida Genomics, Inc. | Self-assembled single molecule arrays and uses thereof |
| US20070168197A1 (en) * | 2006-01-18 | 2007-07-19 | Nokia Corporation | Audio coding |
| CN101432439B (zh) | 2006-02-24 | 2013-07-24 | 考利达基因组股份有限公司 | Dna阵列上的高通量基因组测序 |
| SG10201405158QA (en) * | 2006-02-24 | 2014-10-30 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
| GB0621361D0 (en) * | 2006-10-26 | 2006-12-06 | Fermentas Uab | Use of DNA polymerases |
| WO2008070352A2 (en) | 2006-10-27 | 2008-06-12 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| US20080221832A1 (en) * | 2006-11-09 | 2008-09-11 | Complete Genomics, Inc. | Methods for computing positional base probabilities using experminentals base value distributions |
| US20090111706A1 (en) | 2006-11-09 | 2009-04-30 | Complete Genomics, Inc. | Selection of dna adaptor orientation by amplification |
| RU2414510C2 (ru) * | 2007-06-14 | 2011-03-20 | Институт биохимии и генетики Уфимского научного центра РАН | Способ амплификации специфичных фрагментов нуклеиновых кислот с помощью рекуррентной цепной реакции |
| US7897344B2 (en) * | 2007-11-06 | 2011-03-01 | Complete Genomics, Inc. | Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs |
| US20090263872A1 (en) * | 2008-01-23 | 2009-10-22 | Complete Genomics Inc. | Methods and compositions for preventing bias in amplification and sequencing reactions |
| US8298768B2 (en) * | 2007-11-29 | 2012-10-30 | Complete Genomics, Inc. | Efficient shotgun sequencing methods |
| US8518640B2 (en) * | 2007-10-29 | 2013-08-27 | Complete Genomics, Inc. | Nucleic acid sequencing and process |
| US8415099B2 (en) | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
| WO2009061840A1 (en) * | 2007-11-05 | 2009-05-14 | Complete Genomics, Inc. | Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| WO2009097368A2 (en) | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| US20090270273A1 (en) * | 2008-04-21 | 2009-10-29 | Complete Genomics, Inc. | Array structures for nucleic acid detection |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| US8551708B2 (en) | 2011-02-15 | 2013-10-08 | Leica Biosystems Newcastle Ltd. | Methods for localized in situ detection of mRNA |
| US9556473B2 (en) | 2011-02-15 | 2017-01-31 | Leica Biosystems Newcastle Ltd | Methods for identifying nucleic acid sequences |
| EP2718465B1 (de) | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Verfahren zur vorbereitung eines analyt-arrays |
| AU2012328662B2 (en) | 2011-10-28 | 2015-12-17 | Illumina, Inc. | Microarray fabrication system and method |
| EP2920320B1 (de) | 2012-11-14 | 2016-12-28 | Olink Bioscience AB | Rca-reportersonden und deren verwendung zur detektion von nukleinsäuremolekülen |
| WO2014076209A1 (en) | 2012-11-14 | 2014-05-22 | Olink Ab | Localised rca-based amplification method |
| GB201320145D0 (en) | 2013-11-14 | 2014-01-01 | Olink Ab | Localised RCA-based amplification method using a padlock-probe |
| AU2015259024B2 (en) | 2014-05-16 | 2021-07-01 | Illumina, Inc. | Nucleic acid synthesis techniques |
| HK1243739A1 (zh) * | 2014-12-24 | 2018-07-20 | Abbott Laboratories | 基於目標擴展的信號放大器dna級聯反應的檢測方法 |
| GB201614023D0 (en) | 2016-08-16 | 2016-09-28 | Olink Bioscience Ab | Double-stranded circle probes |
| EP3551765A4 (de) | 2016-12-09 | 2020-11-11 | Ultivue, Inc. | Verbesserte verfahren für multiplex-bildgebung unter verwendung von markierten nukleinsäurekontrastmitteln |
| WO2018183876A1 (en) | 2017-03-31 | 2018-10-04 | Ultivue, Inc. | Dna-antigen exchange and amplification |
| CN116218893A (zh) * | 2023-03-24 | 2023-06-06 | 哈尔滨医科大学 | 一种通过体外扩增重组质粒在其编码基因上生成短片段串联重复序列的方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| CA2087256A1 (en) * | 1990-07-25 | 1992-01-26 | Jerry L. Ruth | Circular extension for generating multiple nucleic acid complements |
| AU8997991A (en) | 1991-01-31 | 1992-08-06 | Becton Dickinson & Company | Exonuclease mediated strand displacement amplification |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| JP3080178B2 (ja) | 1991-02-18 | 2000-08-21 | 東洋紡績株式会社 | 核酸配列の増幅方法およびそのための試薬キット |
| JP3085409B2 (ja) * | 1991-03-29 | 2000-09-11 | 東洋紡績株式会社 | 標的核酸配列の検出方法およびそのための試薬キット |
| GB9117910D0 (en) * | 1991-08-20 | 1991-10-09 | Nat Res Dev | Oligonucleotides and their use in an assay |
| EP0656065A1 (de) * | 1991-10-31 | 1995-06-07 | University Of Pittsburgh | Reverse dot-blot-hybridisierung mittels sonden, die tandem kopf-an-fuss-monomere enthalten, welche durch gestaffelte komlimentäre primer synthetisiert werden. |
| US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
| US6180338B1 (en) * | 1992-08-04 | 2001-01-30 | Beckman Coulter, Inc. | Method, reagent and kit for the detection and amplification of nucleic acid sequences |
| US5876924A (en) | 1994-06-22 | 1999-03-02 | Mount Sinai School Of Medicine | Nucleic acid amplification method hybridization signal amplification method (HSAM) |
| WO1995035390A1 (en) * | 1994-06-22 | 1995-12-28 | Mount Sinai School Of Medicine Of The City University Of New York | Ligation-dependent amplification for the detection of infectious pathogens and abnormal genes |
| US5942391A (en) | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
| AU714486B2 (en) * | 1995-11-21 | 2000-01-06 | Yale University | Unimolecular segment amplification and detection |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
-
1996
- 1996-12-05 CA CA002239287A patent/CA2239287C/en not_active Expired - Fee Related
- 1996-12-05 DE DE69636080T patent/DE69636080T2/de not_active Expired - Lifetime
- 1996-12-05 WO PCT/DK1996/000513 patent/WO1997020948A1/en active IP Right Grant
- 1996-12-05 AT AT03000499T patent/ATE324464T1/de not_active IP Right Cessation
- 1996-12-05 EP EP03000499A patent/EP1300466B1/de not_active Expired - Lifetime
- 1996-12-05 DK DK03000499T patent/DK1300466T3/da active
- 1996-12-05 ES ES03000499T patent/ES2262902T3/es not_active Expired - Lifetime
- 1996-12-05 EP EP96939821A patent/EP0868530B1/de not_active Expired - Lifetime
- 1996-12-05 DK DK96939821T patent/DK0868530T3/da active
- 1996-12-05 AT AT96939821T patent/ATE238434T1/de not_active IP Right Cessation
- 1996-12-05 DE DE69627698T patent/DE69627698T2/de not_active Expired - Lifetime
- 1996-12-05 AU AU76917/96A patent/AU704750B2/en not_active Ceased
-
1999
- 1999-12-17 US US09/465,589 patent/US6610481B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE69636080T2 (de) | 2006-11-30 |
| WO1997020948A1 (en) | 1997-06-12 |
| DE69627698T2 (de) | 2004-01-15 |
| ES2262902T3 (es) | 2006-12-01 |
| EP1300466A3 (de) | 2004-01-02 |
| DK0868530T3 (da) | 2003-07-21 |
| EP0868530A1 (de) | 1998-10-07 |
| ATE238434T1 (de) | 2003-05-15 |
| EP0868530B1 (de) | 2003-04-23 |
| CA2239287A1 (en) | 1997-06-12 |
| CA2239287C (en) | 2008-02-26 |
| AU704750B2 (en) | 1999-05-06 |
| DK1300466T3 (da) | 2006-08-28 |
| AU7691796A (en) | 1997-06-27 |
| US6610481B2 (en) | 2003-08-26 |
| EP1300466B1 (de) | 2006-04-26 |
| US20020031764A1 (en) | 2002-03-14 |
| ATE324464T1 (de) | 2006-05-15 |
| EP1300466A2 (de) | 2003-04-09 |
| DE69627698D1 (de) | 2003-05-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: OLINK AB, UPPSALA, SE |