DE69513178T2 - Verfahren zur sichtung einer bibliothek von chemischen verbindungen - Google Patents
Verfahren zur sichtung einer bibliothek von chemischen verbindungenInfo
- Publication number
- DE69513178T2 DE69513178T2 DE69513178T DE69513178T DE69513178T2 DE 69513178 T2 DE69513178 T2 DE 69513178T2 DE 69513178 T DE69513178 T DE 69513178T DE 69513178 T DE69513178 T DE 69513178T DE 69513178 T2 DE69513178 T2 DE 69513178T2
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- library
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- test
- screening
- compound
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- Expired - Fee Related
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Semiconductor Lasers (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (15)
1. Verfahren zum Absuchen einer auf einer Festphase
bereitgestellten Bank von Verbindungen, umfassend die
Freisetzung eines Teils der Bank von der Festphase in
getrennte Zonen eines Testmediums, Durchführung eines auf
der Nähe der Komponenten basierenden Absuchtests
("proximity screening assay") mit einer biologischen Verbindung
von Interesse in dem Medium, Identifizierung einer (von)
aktiven Zone(n) im Testmedium und Bestimmung der Identität
eines aktiven (aktiver) Mitglieds(Mitglieder) der Bank
durch Bezug auf die entsprechende(n) Verbindung(en), die
noch an die Festphase gebunden ist (sind).
2. Verfahren nach Anspruch 1, wobei die Verbindungsbank eine
Bank chemischer Verbindungen ist.
3. Verfahren nach Anspruch 1 oder 2, wobei der auf der Nähe
der Komponenten basierende Absuchtest ein Fluoreszenztest
ist.
4. Verfahren nach einem der Ansprüche 1 bis 3, wobei der auf
der Nähe der Komponenten basierende Absuchtest ein
Fluoreszenzresonanzenergietransfer (FRET)-Test ist.
5. Verfahren nach Anspruch 1 oder 2, wobei der auf der Nähe
der Komponenten basierende Absuchtest auf der Nähe einer
Radiomarkierung zu einem Scintillator beruht.
6. Verfahren nach einem der vorangehenden Ansprüche, wobei
der auf der Nähe der Komponenten basierende Absuchtest ein
Immuntest ist.
7. Verfahren nach Anspruch 5, wobei die biologische
Verbindung von Interesse und der Scintillator an die
Festphase gebunden sind.
8. Verfahren zum Absuchen einer auf einer Festphase
bereitgestellten Bank chemischer Verbindungen, umfassend die
Freisetzung eines Teiles der Bank von der Festphase in
bestimmte Zonen eines Testmediums und Durchführung eines
Absuchtests mit einer biologischen Verbindung von
Interesse im Medium, Identifizierung einer (von) aktiven
Zone(n) in dem Testmedium und Bestimmung der Identität
eines aktiven (aktiver) Mitglieds (Mitglieder) der Bank
durch Bezug auf die entsprechende(n) Verbindung(en), die
noch an die Festphase gebunden ist (sind).
9. Verfahren nach Ansprüch 8, wobei die Bank eine
kombinatorische Bank chemischer Verbindungen ist.
10. Verfahren nach einem der vorangehenden Ansprüche, wobei
die Festphase eine Vielzahl von individuellen festen
Trägern umfaßt.
11. Verfahren nach einem der vorangehenden Ansprüche, wobei
das Testmedium eine Gelphase umfaßt und die Festphase
durch die Gelphase immobilisiert ist.
12. Verfahren nach einem der vorangehenden Ansprüche, wobei
die Festphase eine Vielzahl von individuellen Kügelchen
mit einer üblichen sichtbaren Markierung umfaßt.
13. Verfahren nach einem der vorangehenden Ansprüche, wobei
das Testmedium zwei oder mehr Schichten umfaßt, wobei jede
Schicht eine oder mehrere Testkomponenten umfaßt.
14. Verfahren nach Anspruch 13, wobei die Bankverbindungen an
oder nahe der Grenzfläche zweier solcher Schichten
freigesetzt werden.
15. Verfahren nach einem der vorangehenden Ansprüche, welches
mehr als einmal unter Verwendung einer Festphase, die der
(den) aktiven Zone(n) vorstehender Runden entspricht,
wiederholt wird.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9414770A GB9414770D0 (en) | 1994-07-22 | 1994-07-22 | Method |
| GBGB9510137.4A GB9510137D0 (en) | 1995-05-19 | 1995-05-19 | Method |
| PCT/GB1995/001700 WO1996003647A1 (en) | 1994-07-22 | 1995-07-19 | Method for screening compound libraries |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69513178D1 DE69513178D1 (de) | 1999-12-09 |
| DE69513178T2 true DE69513178T2 (de) | 2000-08-31 |
Family
ID=26305314
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69513178T Expired - Fee Related DE69513178T2 (de) | 1994-07-22 | 1995-07-19 | Verfahren zur sichtung einer bibliothek von chemischen verbindungen |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0774116B1 (de) |
| AT (1) | ATE186399T1 (de) |
| AU (1) | AU2986395A (de) |
| DE (1) | DE69513178T2 (de) |
| DK (1) | DK0774116T3 (de) |
| ES (1) | ES2139225T3 (de) |
| GB (1) | GB2291708B (de) |
| WO (1) | WO1996003647A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6054557A (en) * | 1995-04-04 | 2000-04-25 | Advanced Bioconcept (1994) Ltd. | Fluorescent peptides |
| US6815423B1 (en) | 1995-07-20 | 2004-11-09 | Perkinelmer Las, Inc. | Fluorescent substance P |
| WO1997045745A1 (en) * | 1996-05-24 | 1997-12-04 | Amersham Pharmacia Biotech Uk Limited | Ex vivo/in vitro assay systems |
| GB9707741D0 (en) | 1997-04-17 | 1997-06-04 | Zeneca Ltd | Process |
| US6455323B1 (en) * | 1997-07-03 | 2002-09-24 | Pharmacia & Upjohn Company | Anti-bacterial methods and materials |
| US5976813A (en) * | 1997-12-12 | 1999-11-02 | Abbott Laboratories | Continuous format high throughput screening |
| DE10125258A1 (de) | 2001-05-23 | 2003-01-09 | November Ag Molekulare Medizin | Verfahren zur Bestimmung des Bindeverhaltens von an Ziel-Molekülen spezifisch bindenden Liganden |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU9201687D0 (en) * | 1992-05-21 | 1992-08-28 | Arpad Furka | Preparation of sets from peptid mixtures with polycomponents and their use for the identification of biologically active peptides |
| WO1994002515A1 (en) * | 1992-07-21 | 1994-02-03 | Bunsen Rush Laboratories Inc. | Oligomer library formats and methods relating thereto |
| IL107166A (en) * | 1992-10-01 | 2000-10-31 | Univ Columbia | Complex combinatorial chemical libraries encoded with tags |
-
1995
- 1995-07-19 DK DK95925915T patent/DK0774116T3/da active
- 1995-07-19 AU AU29863/95A patent/AU2986395A/en not_active Abandoned
- 1995-07-19 EP EP95925915A patent/EP0774116B1/de not_active Expired - Lifetime
- 1995-07-19 WO PCT/GB1995/001700 patent/WO1996003647A1/en active IP Right Grant
- 1995-07-19 GB GB9514722A patent/GB2291708B/en not_active Expired - Fee Related
- 1995-07-19 DE DE69513178T patent/DE69513178T2/de not_active Expired - Fee Related
- 1995-07-19 AT AT95925915T patent/ATE186399T1/de not_active IP Right Cessation
- 1995-07-19 ES ES95925915T patent/ES2139225T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DK0774116T3 (da) | 2000-05-08 |
| ES2139225T3 (es) | 2000-02-01 |
| AU2986395A (en) | 1996-02-22 |
| GB2291708A (en) | 1996-01-31 |
| DE69513178D1 (de) | 1999-12-09 |
| EP0774116B1 (de) | 1999-11-03 |
| EP0774116A1 (de) | 1997-05-21 |
| GB2291708B (en) | 1997-03-05 |
| WO1996003647A1 (en) | 1996-02-08 |
| GB9514722D0 (en) | 1995-09-20 |
| ATE186399T1 (de) | 1999-11-15 |
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