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DE69116921D1 - Verfahren zur Reinigung von monoklonalen Antikörpern - Google Patents

Verfahren zur Reinigung von monoklonalen Antikörpern

Info

Publication number
DE69116921D1
DE69116921D1 DE69116921T DE69116921T DE69116921D1 DE 69116921 D1 DE69116921 D1 DE 69116921D1 DE 69116921 T DE69116921 T DE 69116921T DE 69116921 T DE69116921 T DE 69116921T DE 69116921 D1 DE69116921 D1 DE 69116921D1
Authority
DE
Germany
Prior art keywords
purification
monoclonal antibodies
monoclonal
antibodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69116921T
Other languages
English (en)
Other versions
DE69116921T2 (de
Inventor
Edward M Croze
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ER Squibb and Sons LLC
Original Assignee
ER Squibb and Sons LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ER Squibb and Sons LLC filed Critical ER Squibb and Sons LLC
Publication of DE69116921D1 publication Critical patent/DE69116921D1/de
Application granted granted Critical
Publication of DE69116921T2 publication Critical patent/DE69116921T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69116921T 1990-03-26 1991-03-21 Verfahren zur Reinigung von monoklonalen Antikörpern Expired - Lifetime DE69116921T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/500,340 US5151504A (en) 1989-11-17 1990-03-26 Method for purification of monoclonal antibodies

Publications (2)

Publication Number Publication Date
DE69116921D1 true DE69116921D1 (de) 1996-03-21
DE69116921T2 DE69116921T2 (de) 1996-09-19

Family

ID=23988982

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69116921T Expired - Lifetime DE69116921T2 (de) 1990-03-26 1991-03-21 Verfahren zur Reinigung von monoklonalen Antikörpern

Country Status (5)

Country Link
US (1) US5151504A (de)
EP (1) EP0453767B1 (de)
JP (1) JPH05268985A (de)
CA (1) CA2037204A1 (de)
DE (1) DE69116921T2 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759864A (en) * 1995-06-23 1998-06-02 Cedars Sinai Medical Center Methods for reducing background binding in antibody preparations
US6664379B1 (en) * 1999-09-24 2003-12-16 Ambion, Inc. Nuclease inhibitor cocktail
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
CN100480260C (zh) * 2002-07-18 2009-04-22 克鲁塞尔荷兰公司 抗体混合物的重组生产
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
WO2004106375A1 (en) 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs
JP5732196B2 (ja) * 2006-11-01 2015-06-10 バイオジェン アイデック エムエー インコーポレイティドＢｉｏｇｅｎ Ｉｄｅｃ Ｉｎｃ． 低ｐＨおよび二価カチオンを用いる生物高分子を単離する方法
CA2720048A1 (en) * 2008-04-16 2009-10-22 Biogen Idec Ma Inc. Method of isolating biomacromolecules using polyalkylene glycol and transition metals
WO2012122512A1 (en) 2011-03-10 2012-09-13 Hco Antibody, Inc. Recombinant production of mixtures of single chain antibodies
ES2740749T3 (es) 2012-04-20 2020-02-06 Merus Nv Métodos y medios para la producción de moléculas heterodiméricas similares a Ig
US11180572B2 (en) 2012-07-06 2021-11-23 Genmab B.V. Dimeric protein with triple mutations
SG11201502451QA (en) 2012-09-27 2015-05-28 Merus B V BISPECIFIC IgG ANTIBODIES AS T CELL ENGAGERS
CN105407905B (zh) * 2014-01-03 2020-03-03 生物辐射实验室股份有限公司 蛋白a洗脱产物中杂质的去除
ES2816624T3 (es) 2014-02-28 2021-04-05 Merus Nv Anticuerpos que se unen a EGFR y ERBB3
US11279770B2 (en) 2014-02-28 2022-03-22 Merus N.V. Antibody that binds ErbB-2 and ErbB-3
SI3115376T1 (sl) 2015-07-10 2018-12-31 Merus N.V. Humana protitelesa, ki vežejo CD3
EA201890866A1 (ru) 2015-10-23 2018-09-28 Мерус Н.В. Связывающие молекулы, которые ингибируют рост рака
CA3058343A1 (en) 2017-03-31 2018-10-04 Merus N.V. Erbb-2 and erbb-3 binding bispecific antibodies for use in the treatment of cells that have an nrg1 fusion gene
CN111148764A (zh) 2017-05-17 2020-05-12 美勒斯公司 用于乳腺癌的ErbB-2/ErbB-3双特异性抗体与内分泌治疗的组合
CA3072404A1 (en) 2017-08-09 2019-02-14 Merus N.V. Antibodies that bind egfr and cmet
US20200317783A1 (en) 2017-10-06 2020-10-08 Ono Pharmaceutical Co., Ltd. Bispecific antibody
TWI804572B (zh) 2018-02-09 2023-06-11 日商小野藥品工業股份有限公司 雙特異性抗體
CN108314701A (zh) * 2018-04-09 2018-07-24 青岛汉德森生物科技有限公司 一种利用磁珠提取和纯化抗体的方法
TWI862565B (zh) 2019-04-04 2024-11-21 日商小野藥品工業股份有限公司 雙特異性抗體
CN114702587A (zh) 2019-05-09 2022-07-05 美勒斯公司 用于使蛋白质多聚化的变体结构域及其分离
TWI809286B (zh) 2019-07-05 2023-07-21 日商小野藥品工業股份有限公司 以pd-1/cd3雙特異性蛋白質所進行之血液性癌症治療
JP6856183B1 (ja) 2019-07-30 2021-04-07 小野薬品工業株式会社 二重特異性抗体
EP3772518A1 (de) 2019-08-07 2021-02-10 Merus N.V. Modifizierte menschliche variable domänen
JPWO2021025140A1 (de) 2019-08-08 2021-02-11
CN115666723A (zh) 2020-05-21 2023-01-31 美勒斯公司 用于生产ig样分子的方法和手段
IL318949A (en) 2022-08-23 2025-04-01 Ono Pharmaceutical Co Ltd Bispecific antibody
TW202432587A (zh) 2022-12-27 2024-08-16 荷蘭商美勒斯公司 產生雙特異性蛋白之方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1563355A (en) * 1976-12-14 1980-03-26 Wellcome Found Purification procedure
US4415665A (en) * 1980-12-12 1983-11-15 Pharmacia Fine Chemicals Ab Method of covalently binding biologically active organic substances to polymeric substances
BE896752A (fr) * 1983-05-17 1983-09-16 Region Wallone Representee Pat Procede et chaine reactionnelle pour la diagnostic de la leucose bovine enzootique.
US4704366A (en) * 1984-06-22 1987-11-03 Bio-Rad Laboratories, Inc. Process for binding IgG to protein A
US5082773A (en) * 1986-02-14 1992-01-21 Pharmacia Lkb Biotechnology Ab Cloned streptococcal genes encoding protein G and their use to construct recombinant microorganisms to produce protein G
EP0293391A4 (de) * 1986-02-14 1989-06-21 Genex Corp Klonierte streptokokkengene, kodierend für protein-g und verwendung zur herstellung rekombinanter mikroorganismen zur erzeugung von protein-g.
US4801687A (en) * 1986-10-27 1989-01-31 Bioprobe International, Inc. Monoclonal antibody purification process using protein A
US5089605A (en) * 1987-03-13 1992-02-18 Repligen Corporation Immobilized immunoglobulin-binding proteins
US5084559A (en) * 1987-03-27 1992-01-28 Repligen Corporation Protein a domain mutants
CA1323567C (en) * 1987-10-05 1993-10-26 Gene R. Nathans Method for purification of antibodies

Also Published As

Publication number Publication date
EP0453767A1 (de) 1991-10-30
US5151504A (en) 1992-09-29
EP0453767B1 (de) 1996-02-07
CA2037204A1 (en) 1991-09-27
JPH05268985A (ja) 1993-10-19
DE69116921T2 (de) 1996-09-19

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