[go: up one dir, main page]

DE60226896D1 - Herstellung von f(ab')2 fragmenten in saügetierzelle - Google Patents

Herstellung von f(ab')2 fragmenten in saügetierzelle

Info

Publication number
DE60226896D1
DE60226896D1 DE60226896T DE60226896T DE60226896D1 DE 60226896 D1 DE60226896 D1 DE 60226896D1 DE 60226896 T DE60226896 T DE 60226896T DE 60226896 T DE60226896 T DE 60226896T DE 60226896 D1 DE60226896 D1 DE 60226896D1
Authority
DE
Germany
Prior art keywords
fragments
preparation
tissue cell
antibody fragments
bivalent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60226896T
Other languages
English (en)
Inventor
Abraham Bout
David Halford Jones
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Janssen Vaccines and Prevention BV
Original Assignee
Crucell Holand BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crucell Holand BV filed Critical Crucell Holand BV
Application granted granted Critical
Publication of DE60226896D1 publication Critical patent/DE60226896D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/505Medicinal preparations containing antigens or antibodies comprising antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/60Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/624Disulfide-stabilized antibody (dsFv)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00Uses of viruses
    • C12N2799/02Uses of viruses as vector
    • C12N2799/021Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/022Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from an adenovirus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/10Plasmid DNA
    • C12N2800/108Plasmid DNA episomal vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE60226896T 2001-12-17 2002-12-17 Herstellung von f(ab')2 fragmenten in saügetierzelle Expired - Lifetime DE60226896D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL0100917 2001-12-17
PCT/NL2002/000841 WO2003051927A2 (en) 2001-12-17 2002-12-17 Production of f(ab')2 fragments in mammalian cells

Publications (1)

Publication Number Publication Date
DE60226896D1 true DE60226896D1 (de) 2008-07-10

Family

ID=19760784

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60226896T Expired - Lifetime DE60226896D1 (de) 2001-12-17 2002-12-17 Herstellung von f(ab')2 fragmenten in saügetierzelle

Country Status (11)

Country Link
US (2) US20050048038A1 (de)
EP (1) EP1456238B1 (de)
AT (1) ATE397019T1 (de)
AU (1) AU2002353662B2 (de)
CA (1) CA2470579C (de)
DE (1) DE60226896D1 (de)
DK (1) DK1456238T3 (de)
ES (1) ES2307807T3 (de)
NZ (1) NZ533332A (de)
SI (1) SI1456238T1 (de)
WO (1) WO2003051927A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7604960B2 (en) 1999-04-15 2009-10-20 Crucell Holland B.V. Transient protein expression methods
US7297680B2 (en) 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US8236561B2 (en) 1999-04-15 2012-08-07 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
US7521220B2 (en) 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
US7527961B2 (en) 1999-11-26 2009-05-05 Crucell Holland B.V. Production of vaccines
US7192759B1 (en) 1999-11-26 2007-03-20 Crucell Holland B.V. Production of vaccines
DE60224843T2 (de) 2001-12-07 2009-01-08 Crucell Holland B.V. Herstellung von viren, virusisolaten, und impfstoffen
US20050048038A1 (en) 2001-12-17 2005-03-03 Jones David Halford Ashton Efficient production of f(ab')2 fragments in mammalian cells
KR101183770B1 (ko) 2003-05-09 2012-09-17 크루셀 홀란드 비.브이. E1-불멸화된 세포의 배양물 및 이로부터 생성 수율을 증가시키기 위한 동일물의 배양 방법
MA49426B1 (fr) 2017-10-02 2022-09-30 Laboratorios Silanes S A De C V Processus à haut rendement pour la production d’antivenins à partir de fragments d’anticorps f(ab') 2
WO2023205656A2 (en) * 2022-04-18 2023-10-26 John Paul Ii Medical Research Institute Immortalized cells for production of bioproducts, methods of producing bioproducts with an immortalized cell, and methods of making immortalized cells for production of bioproducts

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4624846A (en) * 1983-07-29 1986-11-25 Immunomedics, Inc. Method for enhancing target specificity of antibody localization and clearance of non-target diagnostic and therapeutic principles
EP0173552B1 (de) 1984-08-24 1991-10-09 The Upjohn Company Rekombinante DNA-Verbindungen und Expression von Polypeptiden wie tPA
US5168062A (en) * 1985-01-30 1992-12-01 University Of Iowa Research Foundation Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence
DE3920358A1 (de) * 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
US7018809B1 (en) * 1991-09-19 2006-03-28 Genentech, Inc. Expression of functional antibody fragments
KR100449181B1 (ko) 1995-06-15 2005-01-24 크루셀 홀란드 비.브이. 유전자요법에사용할수있는사람의재조합아데노바이러스패키징시스템
PT1161548E (pt) * 1999-04-15 2005-06-30 Crucell Holland Bv Producao de proteinas recombinantes numa celula humana utilizando sequencias codificando a proteina e1 de adenovirus
EP1103610A1 (de) * 1999-11-26 2001-05-30 Introgene B.V. Impfstoffherstellung von immortalisierten Säugetierzellinien
EP1130099A1 (de) * 2000-02-25 2001-09-05 Crucell Holland B.V. Aktiviertes Vitronektin als Angiogenesemarker nachgewiesen durch Phagen-Antikörper
US20050048038A1 (en) 2001-12-17 2005-03-03 Jones David Halford Ashton Efficient production of f(ab')2 fragments in mammalian cells

Also Published As

Publication number Publication date
EP1456238B1 (de) 2008-05-28
WO2003051927A3 (en) 2003-11-20
CA2470579C (en) 2012-03-06
DK1456238T3 (da) 2008-08-18
EP1456238A2 (de) 2004-09-15
CA2470579A1 (en) 2003-06-26
SI1456238T1 (sl) 2008-08-31
US20080166767A1 (en) 2008-07-10
US20050048038A1 (en) 2005-03-03
US7537916B2 (en) 2009-05-26
WO2003051927A2 (en) 2003-06-26
ES2307807T3 (es) 2008-12-01
AU2002353662A1 (en) 2003-06-30
ATE397019T1 (de) 2008-06-15
NZ533332A (en) 2005-07-29
AU2002353662B2 (en) 2009-04-23

Similar Documents

Publication Publication Date Title
BR0009964A (pt) Peptìdios artificiais com atividade desuperfìcie e sua utilização na preparação de surfactante artificial
BR9709829A (pt) Formas de dosagem biconvexas de rápida desintegração
DE60226896D1 (de) Herstellung von f(ab')2 fragmenten in saügetierzelle
DK1210411T3 (da) Sammensætninger og fremgangsmåder til forbedret celledyrkning
ATE347585T1 (de) In-vitro wachstum von langerhans'schen inseln und ihre verwendungen
TR200003513T2 (tr) Pirolo[2,3d]Pirimidin kompozisyonları ve kullanımları
ATE246681T1 (de) Sulfonamide
DE69734605D1 (de) Abgeschwächter salmonellenstamm, gebraucht als vehikel zur oralen immunisierung
BR0007781A (pt) Conjugado fisiologicamente ativo, composição, e, processo para a produção de um conjugado de peg-gcsf
ATA165999A (de) Medium zur protein- und serumfreien kultivierung von zellen
DE60037450D1 (de) Funf-helix protein
ES2147720T3 (es) Factor de celulas madre.
NO20061470L (no) Fremgangsmate for fremstilling av gamma-karboksylerte proteiner
DK1379671T3 (da) Fremgangsmåder til dyrkning af circovirus
BR0112070A (pt) Combinações de substâncias ativas com propriedades inseticidas e acaricidas
SI1644484T1 (sl) Avtologne samo-toleranco inducirajoče celice monocitnega izvora in njihova uporaba v farmacevtskihpripravah
PT1451302E (pt) Folhas reconstituídas in vitro de epitélio corneal humano e método de produção das mesmas
BR0109997A (pt) Combinações de substâncias ativas com propriedades inseticidas e acaricidas
PT1200561E (pt) Clone celular recombinante estavel, sua producao e utilizacao
AU2001258373A1 (en) Method for identifying helicobacter antigens
BR0110069A (pt) Combinações de substâncias ativas com propriedades inseticidas e acaricidas
ATE522223T1 (de) Pflanzenextrakte
BR0012751A (pt) Benzofuranos serotonérgicos
PL367306A1 (pl) Szczepionki na bazie autologicznych komórek T i sposoby ich otrzymywania
TR200401462T4 (tr) Torsemid modifikasyonu II' yi içeren dengeli bir farmasötik formülasyonu

Legal Events

Date Code Title Description
8364 No opposition during term of opposition