DE2513944A1 - Verfahren zum extrahieren von nucleinsaeure aus hefezellen - Google Patents
Verfahren zum extrahieren von nucleinsaeure aus hefezellenInfo
- Publication number
- DE2513944A1 DE2513944A1 DE19752513944 DE2513944A DE2513944A1 DE 2513944 A1 DE2513944 A1 DE 2513944A1 DE 19752513944 DE19752513944 DE 19752513944 DE 2513944 A DE2513944 A DE 2513944A DE 2513944 A1 DE2513944 A1 DE 2513944A1
- Authority
- DE
- Germany
- Prior art keywords
- hydrochloric acid
- nucleic acid
- yeast cells
- mass
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 19
- 150000007523 nucleic acids Chemical class 0.000 title claims description 17
- 102000039446 nucleic acids Human genes 0.000 title claims description 17
- 108020004707 nucleic acids Proteins 0.000 title claims description 17
- 210000005253 yeast cell Anatomy 0.000 title claims description 9
- 238000000605 extraction Methods 0.000 title 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 34
- 210000004027 cell Anatomy 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 3
- 229930195733 hydrocarbon Natural products 0.000 claims description 2
- 150000002430 hydrocarbons Chemical class 0.000 claims description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 230000007062 hydrolysis Effects 0.000 description 5
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 229940116269 uric acid Drugs 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000004898 kneading Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 230000007065 protein hydrolysis Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 150000007968 uric acids Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/08—Reducing the nucleic acid content
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT4988474A IT1018642B (it) | 1974-03-29 | 1974-03-29 | Procedimento perfezionato per l estrazione di acidi nucleici in lieviti per alimentazione umana |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2513944A1 true DE2513944A1 (de) | 1975-10-16 |
Family
ID=11271778
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752513944 Pending DE2513944A1 (de) | 1974-03-29 | 1975-03-29 | Verfahren zum extrahieren von nucleinsaeure aus hefezellen |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS50129795A (ja) |
| BE (1) | BE827054A (ja) |
| DE (1) | DE2513944A1 (ja) |
| FR (1) | FR2265854A1 (ja) |
| IT (1) | IT1018642B (ja) |
| NL (1) | NL7503648A (ja) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL423875A1 (pl) * | 2017-12-14 | 2019-06-17 | Eugeniusz Foss | Sposób otrzymywania białka o zmniejszonej zawartości kwasów nukleinowych i białka pozbawionego kwasów nukleinowych |
-
1974
- 1974-03-29 IT IT4988474A patent/IT1018642B/it active
-
1975
- 1975-03-24 BE BE154642A patent/BE827054A/xx unknown
- 1975-03-26 NL NL7503648A patent/NL7503648A/xx unknown
- 1975-03-26 JP JP3664675A patent/JPS50129795A/ja active Pending
- 1975-03-27 FR FR7509675A patent/FR2265854A1/fr active Granted
- 1975-03-29 DE DE19752513944 patent/DE2513944A1/de active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL423875A1 (pl) * | 2017-12-14 | 2019-06-17 | Eugeniusz Foss | Sposób otrzymywania białka o zmniejszonej zawartości kwasów nukleinowych i białka pozbawionego kwasów nukleinowych |
Also Published As
| Publication number | Publication date |
|---|---|
| BE827054A (fr) | 1975-07-16 |
| FR2265854B1 (ja) | 1978-09-29 |
| IT1018642B (it) | 1977-10-20 |
| FR2265854A1 (en) | 1975-10-24 |
| NL7503648A (nl) | 1975-10-01 |
| JPS50129795A (ja) | 1975-10-14 |
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