DE102018003906A1 - Use of polyoxometalates against the infestation of eukaryotic cultures, viral cultures and microorganism populations by mollicutes and mollicutene-inhibiting and killing polyoxometalate-containing substances and processes - Google Patents

Abstract

Extracellular and / or intracellular use of a polyoxometalate- for the prophylaxis and / or after-treatment of mollicutaneous infections and / or mollicutenkontaminationen, - for temporary or permanent killing and / or decontamination of Mollicutenkontaminationen, - for temporary or permanent inhibition of the proliferation of Mollicuten and / or for temporary or permanent adjustment of a constant concentration of mollicuts from and in virus cultures, cultures of unicellular and multicellular eukaryotes and of and in microorganism populations, the polyoxometalate at most in one test culture in the dark or under irradiation with electromagnetic radiation and / or under Atmospheric pressure or overpressure determined limit concentration is applied, in which the Mollicuten are inhibited in their proliferation or killed, but in the polyoxometalate after an incubation time of a Eukaryotentestkultur, a Viru test culture or at least one test population originating from a microorganism population in the dark or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure of 0 hours until permanently and after a Mollicutendetektion after a life in the dark or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure of 0 hours until permanently does not inhibit the proliferation of the respective eukaryotes, the respective host cells of the viruses or of the microorganisms in question.

Description

Field of the invention

The present invention relates to the use of polyoxometalates against the infestation of eukaryotic cultures, viral cultures and microorganism populations by mollicutes, in particular mycoplasmas.

Moreover, the present invention relates to processes for the preparation of mollicutenhemmenden and killing, polyoxometallathaltigen substances against the infestation of eukaryotic cultures, viral cultures and microorganism populations by Mollicutes, in particular by Mycoplasma.

Furthermore, the present invention relates to mollicutene-inhibiting and killing polyoxometalate-containing substances against the infestation of eukaryotic cultures, viral cultures and microorganism populations by mollicutes, in particular mycoplasmas.

Furthermore, the present invention relates to methods for the prevention of infestation of eukaryotic cultures, viral cultures and microorganism populations by Mollicut, for setting a tolerable content of eukaryotic cultures, viral cultures and microorganism populations of Mollicutes and / or for decontamination of mollicut-infested eukaryotic cultures, viral cultures and microorganism populations with polyoxometalate-containing substances.

Furthermore, the present invention relates to kits for the extracellular and / or intracellular application of polyoxometalates for the quantitative detection and control of mollicut, in particular mycoplasma.

Furthermore, the present invention relates to a method for decontamination and sterilization with a cleaning agent which contains at least one polyoxometalate and / or at least one polyoxometalate preparation in dosages ≥ the limiting concentrations according to claim 1.

Not least, the present invention relates to objects and fluids protected against mollicut infestation.

State of the art

The documents cited in the present patent application are incorporated herein by reference.

No problem of cell cultures is as universal as the loss of cell cultures due to contamination. The consequences of cell culture contamination are the loss of time, money and effort, adverse effects on cultures, inaccurate or false experimental results, the loss of valuable products and the embarrassing professional embarrassment.

In particular, mollicuts and especially mycoplasmas play a particularly disastrous role, as they can not be seen under light microscopy and they are resistant to standard antibiotics, so they often remain undetected and adversely affect cellular growth and experimental results. Mycoplasmas significantly affect cell proliferation through nutrient competition and cell toxic secretions. Positively tested cultures often need to be discarded because treatment with antibiotics is usually too costly and their success and safe.

Mollicutes is a class of bacteria. They belong to the department of Tenericutes. Mollicutes are gram negative because they have no cell wall. They represent the smallest and simplest known organisms and they live parasitically on other cells. The tiny Mollicutes sit on or in their host cells and take many of the compounds that they need to live.

The class of mollicutes includes acholeplasmas, mycoplasmas, phytoplasmas and ureaplasmas.

Especially in the family of mycoplasmas you can find pathogens. The genome of the mollicutes is very small and misses numerous genes for the synthesis of vital molecules. This is due - as well as the lack of a cell wall - on the adaptation to the parasitic lifestyle.

Representatives of the Mollicut are unified in research laboratories dreaded contaminants of cell cultures and multicellular eukaryotes, because they can happen due to their small size and flexible cell structure bacteria-proof filters. On average, 30% of all cell cultures are said to contain mycoplasmas.

(see http://www.tagesspiegel.de/magazin/wissen/Krebsforschung;art304,2425114).

The most common contaminants are Mycoplasma hyoorhinis, M. arginii, M. oral and Acholeplasma laidlawaii. The mycoplasmas can alter physiological and morphological parameters of the infected eukaryotic cells and thus influence the results of various experiments. Cell cultures must therefore be regularly examined for contamination.

The Mykoplasmataceae are the only bacterial family of the order Mykoplasmatales. Most species are parasites and pathogens often dangerous to humans and animals.

They have no cell wall, murein is absent. Their genome is very small, which makes them particularly interesting for genetics. Mycoplasma genitalium at 580 kbp was completely sequenced.

The colloquial term Mycoplasma usually refers to the class Mollicutes, not to the family Mycoplasma or the species Mycoplasma specifically. There are two genera in the family: mycoplasma and ureaplasma.

The two genera colonize as parasites exclusively humans and animals. Other genera of the mollicutes such as spiroplasma are also found in insects and plants, e.g. S. apis in bees and some plant species. Most species tolerate oxygen but do not necessarily need it. They are facultative anaerobes. Some species, such as Mycoplasma hyorhinis, can not live in complete exclusion of oxygen. They are obligingly aerobic. The urease test is positive in Ureaplasma, unlike Mycoplasma, Ureaplasma is able to break down urea, which means that they are intracellular bacteria.

The mycoplasmas can usually change their cell shape, they are pleomorphic. The most common cell shape is coccoid, in addition z. B. fungus-like filiform forms observed. Species of Ureaplasma form partially short chains or grape-like clusters.

They live aerobically to facultatively anaerobically and are of varied (pleomorphic), variable, vesicular form. (See Henning (eds.) Brandis under coworker of R. Ansorg Brandis: textbook of the medical microbiology: 192 tables, 7., completely revised edition, G. Fischer, Stuttgart [ua] 1994, ISBN 3437007432, P. 66 , 172, 610ff).

Mycoplasmas are parasitic, intra- and extracellular-living bacteria that cause many diseases in humans, animals and plants. As a rule, bacteria from the class of Mollicutes do not kill their host. Rather, they cause chronic infections, which speaks for a good adaptation to the hosts, and thus embody a very successful type of parasitism.

• - Mykoplasma pneumoniae,
• - Mykoplasma genitalium,
• - Ureaplasma urealyticum,
• - Mykoplasma fermentans,
• - Mykoplasma Mykoides,
• - Mykoplasma agalactiae,
• - Mykoplasma bovis,
• - Mykoplasma capricolum,
• - Mykoplasma conjunctivae,
• - Mykoplasma felis,
• - Mykoplasma gallisepticum,
• - Hämatotrophe Mykoplasmen,
• - Mykoplasma hyopneumoniae,
• - Mykoplasma hyorhinis,
• - Mykoplasma hyosynoviae und
• - Mykoplasma pulmonis.

Mycoplasmas that cause disease include:

• - Mycoplasma pneumoniae,
• - mycoplasma genitalium,
• - Ureaplasma urealyticum,
• - Mycoplasma fermentans,
• - mycoplasma mykoides,
• - mycoplasma agalactiae,
• - mycoplasma bovis,
• - mycoplasma capricolum,
• - mycoplasma conjunctivae,
• - mycoplasma felis,
• - mycoplasma gallisepticum,
• - Hematotrophic mycoplasmas,
• - mycoplasma hyopneumoniae,
• - Mycoplasma hyorhinis,
• - Mycoplasma hyosynoviae and
• - Mycoplasma pulmonis.

• http://www.biocompare.com/Editorial-Articles/170554-Clean-Your-Cultures-with-These-Mykoplasma-Elimination-Tools/
• http://pubs.acs.org/doi/abs/10.1021/cr960396g

See also the links:

• http://www.biocompare.com/Editorial-Articles/170554-Clean-Your-Cultures-with-These-Mykoplasma-Elimination-Tools/
• http://pubs.acs.org/doi/abs/10.1021/cr960396g

Mycoplasmas were first discovered in cell cultures by Robinson and his coworkers in 1956. They sought to examine the effects of PPLO (PleuroPneumoniaLike Organisms), which was the original name for mycoplasma, on HeLa cells (cervical carcinoma human epithelial cells) when they discovered that the control HeLa cultures were already contaminated by PPLO. In addition, they discovered that the other cell lines used at the time in their laboratories were also infected with mycoplasma - a common characteristic of mycoplasma contamination. The mycoplasma tests conducted by the US Food and Drug Administration (ATCC) and by two major cell culture testing organizations currently account for 11 to 15% of mycoplasma cell cultures in the United States are infected. Since many of the cell cultures tested came from laboratories that routinely test for mycoplasmas, the actual level of infection is likely to be significantly higher in many non-test laboratories. It was also found that the degree of mycoplasma infection in Europe was even higher with 25% of 1949 cell cultures from the Netherlands and 37% of 327 cultures from the former Czechoslovakia. The Czechoslovakian studies were particularly interesting because it was found that 100% of the cell cultures were infected by laboratories without mycoplasma test programs but only 2% of the cell cultures of laboratories performing regular tests. It may be even worse in other countries. Other studies have found that 65% of the cell cultures in Argentina and 80% of the cell cultures in Japan were infected by mycoplasma.

Unfortunately, mycoplasmas are not comparably benign cell culture contaminants, but have the ability to adversely affect the functions, growth, metabolism, morphology, attachment, membranes, virus propagation and yield, the induction of interferon and its yield, and chromosomal aberrations and damage as well as cytopathic effects including pathological deposits. The validity of any research on unknowingly infected cell cultures is at least questionable.

1. (i) Diese einfachen, bakterienähnlichen Mikroorganismen sind die einfachsten selbst vermehrenden Organismen, die bekannt sind. Im Durchschnitt haben sie einen Durchmesser von 0,3 bis 0,8 µm.
2. (ii) sie verfügen über keine Zellwand und
3. (iii) sie sind wählerisch, was ihre Wachstumsbedingungen betrifft.

There are three reasons why mycoplasmas have the ability to infect so many cell cultures so easily:

1. (i) These simple, bacterial-like microorganisms are the simplest self-propagating organisms known. On average, they have a diameter of 0.3 to 0.8 μm.
2. (ii) they have no cell wall and
3. (iii) they are picky about their growth conditions.

Their small size and lack of cell walls allow the mycoplasmas to grow to very high densities in eukaryotic cell cultures, with 10 ⁷ to 10 ⁹ colony forming units being common. There were no visible signs of contamination such as cloudiness, pH changes or cytopathic effects.

Because of the medical importance of the mycoplasma problem, numerous methods for the detection of mycoplasma have been developed in recent decades. To mention just two examples of several hundred published patent applications, see the US patent application US 2017/0242007 A1 in which a specific antibody for the immunochromatographic detection of Mycoplasma pneumoniae infections and detection reagents therefor are proposed. Or it is on the European patent EP 1 675 966 B1 in which a method for expression analysis of eukaryotic cells in a cell culture with integrated detection of microorganism contamination in the culture is described. The method comprises (a) providing a microarray having on its surface at least one nucleic acid probe representing a gene of the eukaryotic cell and at least one nucleic acid probe representing a gene of the microorganism, (b) producing nucleic acid targets from said culture by means of a primer mixture which is suitable for amplifying said at least one gene of a eukaryotic cell and said at least one gene from a microorganism, (c) bringing the microarray from the process step (a) into contact with the nucleic acid targets from process step (b) to allow selective hybridization between the nucleic acid targets and their complementary nucleic acid probes on the microarray; and (d) detection of said hybridization, detecting microorganism contamination and, optionally, detection of expression of genes specific for the eukaryotic cell All are.

The large number of patent applications dealing with the detection of mycoplasmas faces a much smaller number of patent applications dealing with the prophylaxis of mycoplasma infections and the decontamination of mycoplasmas in cell cultures.

Thus, according to the international patent application WO 2015/123728 A1 immunogenic fragments of a mycoplasma XAA aminopeptidase and mutants of aminopeptidase proteins with partially inactivated active sites and metal binding residues provided. Furthermore, antibodies such as mycoplasma XAA aminopeptidase are also provided. Mycoplasma infections and contaminations are detected, prevented and / or treated in animals or in cell cultures.

From the American patent application US 2004/0053847 A1 For example, diastereomeric peptides are known which are useful for the treatment of cancer and for the topical treatment of infections caused by pathogenic organisms such as bacteria and fungi. In addition, they can be used as food additives instead of antibiotics for pet food for the control of mycoplasma infections and food preservation.

From the translation of the European patent specification EP 0 348 947 B1 . DE 689 10 397 T2 , there is known a method for preventing or eliminating mycoplasma contamination of animal or plant cell cultures comprising the preparation of an antimycoplastically effective amount of 5-amino-7- (2-aminomethylmorpholino) -1-cyclopropyl-6,8-difluoro-1,4- dihydro-oxoquinoline-3-carboxylic acid or its salt is added to a culture medium in which animal or plant cells are cultured.

From the German patent application DE 36 17 803 A1 It is known to use the gyrase inhibitors quinolone and 1,8-naphthyridone-3-carboxylic acids for the decontamination of mycoplasma-infected cell cultures.

From the German patent application DE 35 39 393 A1 The use of ciprofloxacin for the decontamination of mycoplasma-infected cell cultures is known.

For the same purpose, in the US patent US 4,546,097 Digitonin and digitonin-related saponins have been proposed as mycoplasma suppressors in cell cultures.

There are currently two groups of mycoplasma cell culture products on the market:

Mynox® and Mynox®Gold from Biochrom:

The treatment should include two steps: The "starter treatment" leads to a massive reduction in vital mycoplasmas. The active ingredient is a dissolved biosurfactant which is added directly to the contaminated cell or virus culture. This drug is said to show a high affinity for mycoplasma membrane. Unlike other prokaryotes, mycoplasmas do not have a cell wall but are surrounded by a lipid membrane. The interaction of Mynox®Gold with this lipid membrane induces permeability changes and increases the efficacy of the antibiotic portion. The eukaryotic cell is only affected at significantly higher drug concentrations. By changing the medium, the reagents are removed from the culture and permanently kills any surviving mycoplasma by three treatments with the Main Treatment.

MykoRAZOR® from Biontex:

MykoRAZOR® should be effective even in very low concentrations. Its effect is based on the intervention in the protein biosynthesis (inhibition of translation by the binding to the ribosomes), as well as in the transcription apparatus of mycoplasmas (gyrase inhibitors) and this without affecting the eukaryotic cells in culture. MykoRAZOR® is added to the culture medium and is intended to quickly and completely kill mycoplasma and bacteria in the culture. However, this requires mixtures or sequential treatment cycles with specific antibiotics such as neomycin, tetracyclines, macrolides, quinolones and gentramycin.

Polyoxometalates are inorganic polyacids consisting of two groups: heteropolyacids and isopolyacids.

Heteropoly acids arise from weak, polybasic oxo acids of a metal (usually chromium, molybdenum, vanadium or tungsten) and a non-metal (usually arsenic, iodine, phosphorus, selenium, silicon or tellurium) as partial mixed anhydrides; Example: H ₃ [PM ₁₂ O ₄₀ ]: 12-molybdophosphoric acid (M = Mo) or 12-tungstophosphoric acid (M = W). Actinides or lanthanides can also act as the second central atom; The tungsten heteropolyacids are thermally much more stable than the analogous molybdenum compounds. Heterocyclic acids of the general formula [(EO ₄ ) M ₁₂ O ₃₆ ) _n-8 with n = valence of the tetrahedrally coordinated element E (eg boron, silicon, zinc) are sometimes referred to as Keggin acids. With octahedral coordinated heteroatom, the heterohexametallate type [(EO ₆ ) M ₆ O ₁₈ ] _n-12 (Anderson-Evans anions) is frequently found [cf. Römpp Online, Version 3.47 "Heteropolyacids"].

Isopolyacids or homopolyacids are partial anhydrides which, in contrast to the heteropolyacids, contain only central atoms of one kind. In particular, molybdate and tungstates and oxometalates of some other transition metals tend to form isopoly acids with dehydration such as H ₄ [Mo ₈ O ₂₆ ] or H ₄ [Wo ₁₀ O ₃₂ ] [cf. Römpp Online, Version 3.81 "Isopolyacids"].

In her article "Fabrication and Characterization of Antibacterial-active Multilayer Films Based on Keggin Polyoxometalates and Methylene Blue," in Z. Naturforsch. 2010, 65b, pages 140-146, describe Dan Chen, Jun Peng, Haijun Pang, Pengpeng Zhang, Yuan Chen, Yan Shen, Chanyung Chen, and Huiyuan Ma, multi-layered films based on the Keggin polyoxometalates alpha (SiW ₁₂ O ₄₀ ] ^4- / alpha [PMo ₁₂ O ₄₀ ] ^3- , which show antibacterial activity against Escherichia coli.

In their article "Enhancement of antibacterial activity of beta-lactam antibiotics by [P ₂ W ₁₈ O ₆₂ ] ^6- , [SiMo ₁₂ O ₄₀ ] ^4- , and [PTi ₂ W ₁₀ O ₄₀ ] ^7- against methicillin-resistant and vacomycin resistant Staphylococcus aureus "in Journal of Inorganic Biochemistry, 100 (2006), pages 1225 to 1233, Miyauo Inue, Tokomo Suzuki, Yutaka Fujita, Mayumi Oda, Nobuhiro Matsumato and Thoshihiro Yamase describe increasing the antibacterial activity of beta-lactam antibiotics the above-mentioned heteropolyacids.

In its article "Antibacterial activity of highly negatively charged polyoxotentates, K ₂₇ [KAs ₄ W ₄₀ O ₁₄₀ ] and K ₁₈ [KSb ₉ W ₂₁ O ₈₆ ], and Keggin-structural polyoxotungstates agaist Helicobacter pylori", in Journal of Inorganic Biochemistry, 99 (2005), pp. 1023-1031, Miyao Inoue, Keiko Segawa, Sae Matsunaga, Nobuhiro Matsumoto, Mayumi Oda, and Toshihiro Yamase describe the antibacterial activity of these polyoxometalates (POM) based on determination of minimum inhibitory concentration (MIC), and the fractional inhibitory concentration (FIC), the time of death of the bacteria, the bacterial morphology and the uptake of the POM into the bacterial cells.

In their article "Fabrication and characterization of multilayer films based on Keggin-type polyoxometalate and chitosan, in Materials Letters, 60 (2006), pages 1588 to 1593, Yuhua Feng, Zhangan Han, Jun Pen, Jun Lu, Bo Xue, Li Li, Huiyuan Ma and Enbo Wang multilayer films based on the Keggin-type polyoxometalates alpha- [SiW ₁₂ O ₄₀ ] ^4- and alpha- [PMo ₁₂ O ₄₀ ] ^3- and cationic chitosan.

Shuiping Chen, Guozhong Wu, Dewu Long and Yaodang Liu describe in their article "Preparation, characterization and antibacterial activity of chitosan-Ca ₃ V ₁₀ O ₂₈ complex membrane", in Carbohydrate Polymers, 64 (2006), pages 92 to 97 Chitosan-Ca ₃ V ₁₀ O ₂₈ complex membrane with persistent antimicrobial action. The membrane is prepared by the self-assembly of V1 ₀ O ₂₈ ⁶ "and chitosan using Ca ²⁺ as the linker.

In their article "Studies on the first antibacterial agent of pipemidic acid-modifying Keggin polyoxometalate" in Inorganic Chemical Communication, 14, pages 1192 to 1195, 2011, C. Li et al. an adduct of POM with pipemidic acid (HPPA) of the formula {[Co (PPA) ₂ ] H ₂ [SiW ₁₂ O ₄₀ ]} · HPPA.3H ₂ O and its antitumor effect on MCF-7 cells.

• - [Cu(II)(Enrofloxacin)₂(H₂O)₂]H₂[b-Mo₈O₂₆].4H₂O,
• - [Cu(II)₂(Pipemidinsäure)₄] [d-Mo₈O₂₆].4H₂O,
• - [Cu(II)₂(Norfloxacin)₂(H₂O)₂] [b-Mo₈O₂₆] und
• - [Cu(II)₂(Enoxazin)₂(H₂O)₄] [b-Mo₈O₂₆].2H₂O.

In their article "Study on the ligation of copper complexes of the quinolone antibacterial drugs and octamolybdates" in Polyhedron 31, pages 422 to 430, 2012 describe J.-Q. Sha et al. the antitumor activity of

• - [Cu (II) (enrofloxacin) ₂ (H ₂ O) ₂ ] H ₂ [b-Mo ₈ O ₂₆ ] .4H ₂ O,
• - [Cu (II) ₂ (pipemidic acid) ₄ ] [d-Mo ₈ O ₂₆ ] .4H ₂ O,
• - [Cu (II) ₂ (norfloxacin) ₂ (H ₂ O) ₂ ] [b-Mo ₈ O ₂₆ ] and
• - [Cu (II) ₂ (enoxazine) ₂ (H ₂ O) ₄ ] [b-Mo ₈ O ₂₆ ] .2H ₂ O.

In their article "Studies on the interactions of Ti-containing polyoxometalates (POMs) with SARS-CoV 3Clpro by molecular modeling" in Journal of Inorganic Biochemistry, 101, pages 89 to 94, 2007, D. Hu et al. the SARS activity of the isomers of [a-PTi ₂ W ₁₀ O ₄₀ ] ⁷

In their article "Antibacterial activity of some saturated polyoxotungstates" in Revista Romana de Medicina de Laborator, Vol. 22, No. 1, Martie, 2014 report L. Grama, A. Man, D.-L Muntean, SA Gaze Florea, F. Boda and A. Curticacepan on the antibacterial activity of [H ₄ [Si (W ₃ O ₁₀ ) 4] .xH ₂ O and [Na ₃ [P (W ₃ O ₁₀ ) ₄ ] .xH ₂ O against Staphylococcus spp and especially MRSA. They point out that comparatively high concentrations are needed

From the American patent application US 2004/0185078 A1 , the American patent US 6,713,076 B1 , the European patent EP 1 078121 B1 and the European patent application EP 1439261 A2 discloses a method of removing gaseous phase or liquid phase contaminants by contacting a cellulosic fiber-based material with embedded POM with the contaminated gas phase or liquid phase.

From the international patent application WO 2015/034 9007 curable dental mixtures are known which contain polyoxometalates and / or derivatives thereof.

From the American patent US 6,911,470 B1 POM are known to have antiretroviral activity.

From the American patents US 5,824,706 and US 6,020,369 are the prevention and treatment of respiratory viral infections known in which a POM-containing aerosol spray is applied to the lungs.

From the American patent application US 2008/0187601 A1 and the American patents US 6,723,349 B2 and US Pat. No. 7,097,858 B2 are topical POM-containing compositions are known by means of which pollutants, in particular warfare agents, are removed from the environment. In addition, the topical compositions may also contain cerium, silver, gold or platinum compounds. In particular, perfluoropolyethers (PFPE) can be used as the carrier. Thus, the warfare agent 2-chloroethylethyl sulfide (CEES) in the presence of POM as catalysts can be quantitatively oxidized to 2-chloroethylethyl sulfoxide (CEESO). These known topical compositions have the disadvantage that they can not be removed from the skin by water.

From the international patent application WO 2006/036269 A2 For example, microspheres of particle size from 1 to 2000 microns are known to contain a hydrophilic polymer such as oxidized cellulose having numerous pendent anionic groups and POM. The microspheres are used for fixation and dosing of therapeutic radioisotopes.

Romanian Patent 122728 discloses a process for bleaching natural fibers with oxygen using POM as catalysts.

The Moldovan patent MD 4014 B1 discloses POM with antitumor effect.

From the American patent US 6,387,841 B1 For example, catalysts for the conversion of alkanes to unsaturated compounds containing oxidic catalysts supported on polyoxometalates are known.

From the American patents US 6,043,184 . US 6,196,202 B1 and US 5,990,348 For example, catalysts are known for converting alkanes to unsaturated carboxylic acids containing polyoxometalates supported on large pore polyoxometalates.

From the American patent US 6,596,896 B2 For example, a process for the production of an aromatic carbonate by the reaction of an aromatic monohydroxy compound with carbon monoxide and oxygen is known. The reaction is carried out in the presence of a palladium compound, a redox catalyst, a polyoxometalate and a quaternary ammonium or phosphonium salt.

From the American patent US 8,129,069 B2 For example, a composite is known as a fuel cell component that contains a proton-conducting polymer, a water-insoluble proton-conducting inorganic material, and a polyoxometalate.

From the American patent US 5,904,734 For example, a bleaching agent containing peroxide and an activator is known. The activator may be tungsten silicic acid. The bleach can remove mold and fungus from tiles and curtains.

From the Japanese patent application JP 002005281299 A For example, the use of potassium salt of 12-silicotungstic acid as an antibacterial and antifungal agent for use in furniture coating compositions is known.

In the article by Eunyoung Bae, Jae Won Lee, Byeong Hee Wang, Jiman Yeo, Jecong Yoon, Hyung Joon Cha and Wonyong Choi "Photocatalytic bacterial inactivation by polyoxometalates", in Chemosphere 72 (2008), 174-181, the photocatalytic inactivation becomes of Gram-negative and Gram-positive bacteria. For this purpose, aqueous suspensions of the bacteria containing H ₄ SiW ₁₂ O ₄₀ or H ₃ PW ₁₂ O _{40 were} irradiated with UV light. The authors conclude that the polyoxometalates act as photocatalysts.

From the American patent application US 2009/0004124 A1 For example, cosmetic compositions containing fluidized particles such as H ₃ PW ₁₂ O ₄₀ are known. The fluidization of the particles is carried out by residues of long carbon chains, which are linked to the particles.

From the American patent application US 2009/0012204 A1 For example, polymers functionalized with reactive polyoxometalates are known which serve to decontaminate harmful compounds. As polyoxometalates, those of the Keggin type of the general formula (R ^{m +} ) _y [X ^{n +} M ₁₂ O ₄₀ ] ^{(8-n) -} , wherein m and n are integers, y = (8-n) / m, X = Phosphorus or silicon and M = molybdenum, tungsten, vanadium and mixtures thereof, O = oxygen and R = hydrogen, silver, ammonium, quaternary ammonium and mixtures thereof.

From the American patent application US 2009/0022645 A1 is the use of K ₄ [α-SiW ₁₂ O ₄₀ ] known as an activator for bleaching.

From the German patent application DE 10 2013 104 284 A1 are known antimicrobial composites containing inter alia polyoxometalates. The composite materials can be used for household goods, consumer goods, industrial equipment and components, marine paints, facade paints, tanks, cables, coatings, pipes, pipes, components of oil exploration, oil production and oil storage, medical technology, food technology, sanitary facilities, packaging, textiles, furniture, building elements, furnishings , Latches, switches, seats, keyboards and equipment used by clinics, medical practices, nursing homes, day care centers, schools and publicly accessible buildings.

Except for the German disclosure DE 10 2015 000 812 A1 cleaning and care agents are known which contain polyoxometalates.

Furthermore, from the German patent application DE 10 2015 000 813 A1 the use of polyoxometalates for the decomposition of chemotherapeutic agents and other drugs that are excreted through the skin.

• - Akarizide gegen Milben,
• - Algizide gegen Algen,
• - Bakterizide und Bakteriostatika gegen Bakterien und Bakterienfilme,
• - Fungizide gegen Pilze,
• - Insektizide gegen Insekten,
• - mikrobiozide Ausrüstung gegen Keime,
• - Molluskizide gegen Schnecken,
• - Nematizide gegen Fadenwürmer und
• - Viruzide gegen Viren

verwendet werden. Es wird auch ganz allgemein angegeben, dass die bioziden Ausrüstungen in Laboratorien, insbesondere biologische und mikrobiologische Menschen Laboratorien, Zellkulturen, Nährmedien und Krankenhäusern, insbesondere Operationssäle, verwendet werden können. Der gezielte Einsatz gegen Mollicuten wird nicht erwähnt. Not least is from the German disclosure DE 10 2015 000 814 A1 the biocidal equipment of articles with polyoxometalate micro- and / or nanoparticles known. Specifically, the biocidal equipments containing polyoxometalates can be described as

• - acaricides against mites,
• - algicides against algae,
• - Bactericides and bacteriostats against bacteria and bacterial films,
• - fungicides against fungi,
• Insecticides against insects,
• - microbiocidal equipment against germs,
• - molluscicides against snails,
• - Nematicides against roundworms and
• - Viruzide against viruses

be used. It is also generally stated that the biocidal equipment can be used in laboratories, in particular biological and microbiological human laboratories, cell cultures, culture media and hospitals, in particular operating theaters. The targeted use against Mollicutes is not mentioned.

From the Japanese patent application JP 002005281299 A discloses bactericidal and fungicidal coating materials containing Keggin-type heteropolyacids, [XM ₁₂ O ₄₀ ] ⁿ , and the Dawson type, [X ₂ M ₁₈ O ₆₂ ], wherein X = silicon, phosphorus, or boron.

From the American patent US 7,211,707 B2 For example, reactive and adsorptive materials are known which contain activated carbon absorbers in which nanoparticles are embedded. The materials are biocidal and can neutralize and decompose harmful chemicals. In addition to numerous other nanoparticles, polyoxometalates are also used.

From the European patent EP 2 765 136 A1 Heteropolyoxometallate are known, which can be used in substances, surface layers, paints or coatings for disinfection purposes antimicrobial purposes.

In the article of JT Rhule, CL Hill and DA Judd "Polyoxometalates in Medicine" in Chemical Reviews 1998, 98, pages 327-357 , the authors refer to the previously known studies on the antiviral and anticancer activity of polyoxometalates.

The Gibco® IPL-41 Insect Medium (1X) contains inter alia ammonium heptamolybdate tetrahydrate, [(NH ₄ ) ₆ Mo ₇ O ₂₄ ] .4H ₂ O, CAS-No. 12054-85-2 (tetrahydrate), AHMT, as part of the formulation.

(See https://www.fishersci.ca/shop/products/gibco-ipl-41-insect-medium-1x/11405081)

However, the concentration of AHMT is not indicated. There is also little evidence that AHMT is particularly toxic to mollicut, especially mycoplasma.

It is therefore more likely that AHMT is added to the medium because molybdenum is essential for almost all organisms and forms the catalytic center of a large number of enzymes such as nitrogenases, nitrate reductases, sulfide toxidases and xanthine oxidoreductases (see Nature, 839-847 (1994); August 13, 2009) / doi: 10.1038 / nature08.3.2002, published online on August 12, 2009, article: Molybdenum Cofactors, enzymes and pathways, Günter Schwarz, Ralf R. Mendel and Markus W. Ribbe).

From the state of the art relating to the polyoxometalates, there are therefore no studies on their effect on mollicutes, in particular mycoplasmas. Of course, it can be assumed that the killing of unicellular and multicellular eukaryotes kills the mollicutes as a side effect as well. However, this does not solve the problem of killing the Mollicutes and letting the Eukaryotes live on.

These problems are also present in virus cultures. As is known, because of the lack of metabolism, viruses can only multiply in a culture of suitable host cells. Therefore, the appropriate eukaryotic cells are used, and bacteriophages have the appropriate bacterial cells. The virus cultures can therefore also be attacked by Mollicutes. It is true that the prior art relating to the polyoxometalates discloses the use of these compounds for the treatment of viral diseases. However, there are no suggestions or hints that Mollicuts can be targetedly killed without damaging the virus cultures.

Disadvantages of the prior art

1. (i) Durch Minox® werden nur extrazelluläre Mykoplasmen abgetötet oder in ihrem Wachstum gehemmt. Intrazelluläre Mykoplasmen werden dagegen nicht abgetötet.
2. (ii) Behandlungszyklen mit spezifischen Antibiotika (MykoRAZOR®) haben das Problem, dass sie nicht vollständig Mykoplasmen eliminieren können. Ebenso entstehen gefährliche Resistenzen. Des Weiteren werden die Zellen geschwächt, sodass große Teile - manchmal 80 % oder mehr - absterben.
3. (iii) All diese Verbindungen haben den Nachteil, dass sie temperaturempfindlich sind und über Kühlketten transportiert und gelagert werden müssen. Die Verbindungen haben eine definierte endliche Haltbarkeit um Gebrauchsdauer und sind sehr temperaturempfindlich und verlieren dadurch bedingt auch ihre Aktivität in der Zellkultur.
4. (iv) Außerdem beeinflussen die Antibiotika die Zellen erheblich, sodass die mit ihnen erzielten Ergebnisse fraglich sind.
5. (v) Des Weiteren muss nach einer Behandlung eine Erholungszeit für die Zellen eingehalten werden, wobei es nicht immer gewährleistet ist, dass sich die Zellen nicht bereits dauerhaft verändert haben.
6. (vi) Ferner sind die Antibiotika nicht als Breitbandantibiotika gegen die verschiedenen Mykoplasmen einsetzbar.
7. (vii) Es gibt bisher keine Stoffe, die spezifisch gegen Mykoplasmen wirken, aber zugleich nicht bakterizid oder bakteriostatisch sind.

The disadvantages of the prior art are numerous:

1. (i) Minox® kills or inhibits growth of extracellular mycoplasmas. Intracellular mycoplasmas, however, are not killed.
2. (ii) Treatment cycles with specific antibiotics (MykoRAZOR®) have the problem that they can not completely eliminate mycoplasma. Likewise, dangerous resistances arise. Furthermore, the cells are weakened, causing large parts - sometimes 80% or more - to die.
3. (iii) All these compounds have the disadvantage that they are sensitive to temperature and must be transported and stored via cold chains. The compounds have a defined finite shelf life and are very sensitive to temperature and therefore also lose their activity in the cell culture.
4. (iv) In addition, the antibiotics affect the cells significantly, so that the results obtained with them are questionable.
5. (v) Furthermore, after a treatment, a recovery time for the cells must be maintained, although it is not always guaranteed that the cells have not already changed permanently.
6. (vi) Furthermore, the antibiotics can not be used as broad spectrum antibiotics against the various mycoplasmas.
7. (vii) So far, there are no substances that are specific to mycoplasma, but are not bactericidal or bacteriostatic at the same time.

Object of the present invention

The present invention was therefore based on the object to find temperature-insensitive, transportable and long-term storable substances that are specifically against Mollicutes, especially Mycoplasma, effective.

Specifically, the fabrics should be effective against mollicutes, especially mycoplasmas, in virus cultures and single cell and / or multicellular eukaryotic cultures. Cultures may include cell cultures, 3D cell cultures, organ-on-a-chip, 3D tissue culture, microbiological nutrient systems, culture media, tissue culture, and adjuvant media, as well as viruses and unicellular and / or multicellular eukaryotes present in and / or on Equipment, apparatus and laboratories for biochemistry, molecular biology, genetic engineering, cell biology microbiology, virology, pharmacology, toxicology and medicine.

These substances are intended as contamination prophylaxis to prevent the infestation or infection of cultures by the Mollicutes, in particular mycoplasmas, and decontaminate infested cultures by killing the Mollicutes, especially mycoplasmas, or at least inhibit their multiplication, so that at least a constant and harmless Concentration of Mollicutes in the virus cultures and eukaryotic cultures sets. These substances should not alter their original properties, inhibit their propagation or even kill existing viruses and eukaryotes, so that the research results obtained on and with the cultures are valid.

Furthermore, the substances of Mollicutes, especially mycoplasmas, are said to decontaminate infected or infected populations of microorganisms residing in and / or on equipment, apparatus and laboratories for cell biology, microbiology, pharmacology, toxicology and medicine, so that they do not provide sources for one Infest cultures by mollicutes, especially mycoplasmas, more represent.

Inventive solution

• - für die Prophylaxe und/oder die Nachbehandlung von Mollicuteninfektionen und/oder Mollicutenkontaminationen,
• - zur temporären oder dauerhaften Abtötung und/oder Dekontaminierung von Mollicutenkontaminationen,
• - zur temporären oder dauerhaften Inhibierung der Vermehrung von Mollicuten und/oder
• - zur temporären oder dauerhaften Einstellung einer konstanten Konzentration von Mollicuten

von und in Viruskulturen, Kulturen einzelliger und mehrzelliger Eukaryoten sowie von und in Mikroorganismenpopulationen, wobei

• - das mindestens eine Polyoxometallat höchstens in einer an jeweils mindestens einer Testkultur im Dunkeln oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck ermittelten Grenzkonzentration angewandt wird, in der
• - die Mollicuten in ihrer Proliferation inhibiert oder abgetötet werden, in der aber
• - das mindestens eine Polyoxometallat nach einer Inkubationzeit einer gegebenen Eukaryotentestkultur, einer gegebenen Virustestkultur oder einer von einer gegebenen Mikroorganismenpopulation stammenden mindestens einenTestkultur im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck von 0 Stunden bis auf Dauer und nach mindestens einer Mollicutendetektion nach einer Standzeit im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck von 0 Stunden bis auf Dauer die Proliferation der betreffenden Eukaryoten, der betreffenden Wirtszellen der Viren oder der betreffenden Mikroorganismen noch nicht inhibiert, gelöst.

According to the invention, this object has been achieved by the extracellular and / or intracellular use of at least one polyoxometalate

• for the prophylaxis and / or aftercare of mollicut infections and / or mollicut contamination,
• for the temporary or permanent killing and / or decontamination of mollicut contaminants,
• for the temporary or permanent inhibition of the proliferation of Mollicutes and / or
• - For temporary or permanent adjustment of a constant concentration of Mollicutes

and in virus cultures, cultures of unicellular and multicellular eukaryotes, and of and in microorganism populations, wherein

• - the at least one polyoxometalate is used at most in a limit concentration determined in each case in at least one test culture in the dark or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure, in which
• - The proliferation of Mollicut be inhibited or killed, but in the
• the at least one polyoxometalate after an incubation time of a given eukaryotic test culture, a given virus test culture or at least one test culture originating from a given microorganism population in the dark and / or under exposure to electromagnetic radiation and / or under atmospheric pressure or overpressure from 0 hours to permanent and after at least a Mollicutendetektion after a life in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure of 0 hours to permanent proliferation of the eukaryotes concerned, the respective host cells of the virus or the microorganisms in question not yet resolved solved.

In the following, the extracellular and / or intracellular use of at least one polyoxometalate is referred to as "use according to the invention".

• - die Kulturen von Arzneimitteln, zellbiologischen Nährsystemen, zellbiologischen Nährmedien, Gewebekulturen, 3D-Gewebekulturen, Organen, Organtransplantaten, künstlich hergestellten Organen, Organen „on a chip“, Hilfsmedien, Laboratorien für die Biochemie, Molekularbiologie, Gentechnologie Zellbiologie Mikrobiologie, Virologie, Pharmakologie, Toxikologie und Medizin sowie von Gegenständen, Möbeln, Abzügen, Kleidung, Gerätschaften und Apparaturen für und in diesen Laboratorien stammen und wobei
• - das mindestens eine Polyoxometallat höchstens in einer an jeweils mindestens einer Testkultur im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck ermittelten Grenzkonzentration anwendbar ist, in der
• - die Mollicuten in ihrer Proliferation inhibiert oder abgetötet sind, in der aber
• - das mindestens eine Polyoxometallat nach einer Inkubationzeit einer gegebenen Eukaryotentestkultur, einer gegebenen Virustestkultur oder einer von einer gegebenen Mikroorganismenpopulation stammenden mindestens einenTestkultur im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck von 0 Stunden bis auf Dauer und nach mindestens einer Mollicutendetektion nach einer Standzeit im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck von 0 Stunden bis auf Dauer die Proliferation der betreffenden Eukaryoten, der betreffenden Wirtszellen der Viren oder der betreffenden Mikroorganismen noch nicht inhibiert ist.

Furthermore, this object has been achieved in accordance with the invention by the cultures of unicellular and / or multicellular eukaryotes, virus cultures and cultures of microorganism populations that are free of proliferation-capable mollicut-containing, at least one polyoxometalate, wherein

• - cultures of medicinal products, cell biological nutrient systems, cell culture media, tissue cultures, 3D tissue cultures, organs, organ transplants, man-made organs, on-chip organs, auxiliary media, biochemistry laboratories, molecular biology, genetic engineering cell biology microbiology, virology, pharmacology, Toxicology and medicine, as well as objects, furniture, prints, clothing, implements and equipment for and in these laboratories
• the at least one polyoxometalate is applicable at most in a limit concentration determined in each case in at least one test culture in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure, in which
• - The mollicutes are inhibited or killed in their proliferation, but in the
• the at least one polyoxometalate after an incubation time of a given eukaryotic test culture, a given virus test culture or at least one test culture originating from a given microorganism population in the dark and / or under exposure to electromagnetic radiation and / or under atmospheric pressure or overpressure from 0 hours to permanent and after at least a Mollicutendetektion after a lifetime in the dark and / or under irradiation with electromagnetic radiation and / or atmospheric pressure or overpressure of 0 hours to permanent proliferation of the respective eukaryotes, the respective host cells of the virus or microorganisms concerned is not yet inhibited.

In the following, the proliferating mollicut-free cultures containing at least one polyoxometalate are referred to as "cultures according to the invention".

• (A) mindestens eine Vorrichtung zur Durchführung spektroskopischer, mikrobiologischer und/oder chemischer Messungen zum qualitativen und/oder quantitativen Nachweis von einzelligen Eukaryoten, mehrzelligen Eukaryoten, Wirtszellen von Viren, Mikroorganismenpopulationen und Mollicuten an jeweils mindestens einer Testprobe, die mindestens einer mit mindestens einer Art von Mollicuten infizierten Testkultur, enthaltend mindestens eine Art von einzelligen Eukaryoten und/oder mindestens eine Art von mehrzelligen Eukaryoten, mindestens einer Art von Wirtszellen oder mindestens einer Art von Mikroorganismenpopulationen, entnehmbar ist,
• (B) mindestens eine Vorrichtung zur Auswertung und Speicherung der an der mindestens einen Testprobe gemessenen Testergebnisse,
• (C) von mindestens einer Art von Mollicuten freie mikrobiologische Nährmedien, Nährsysteme, Hilfsmedien, Gerätschaften und Apparaturen zur Herstellung mindestens einer von mindestens einer Art von Mollicuten freien Kultur, enthaltend
  • (C1) mindestens eine Art von einzelligen Eukaryoten und/oder mindestens eine Art von mehrzelligen Eukaryoten,
  • (C2) mindestens eine Art von Wirtszellen für Viren oder
  • (C3) mindestens eine Art von Mikroorganismenpopulationen, als Null-Probe, wobei die Eukaryoten, die Wirtszellen und Viren oder die Mikroorganismenpopulation von Arzneimitteln, Zellkulturen, 3D-Zellkulturen, zellbiologischen Nährsystemen, zellbiologischen Nährmedien, Gewebekulturen, 3D-Gewebekulturen, Organen, Organtransplantaten, künstlich hergestellten Organen, Organen „on a chip“, Hilfsmedien sowie Abstrichen von Laboratorien für die zellbiologischen Nährsystemen, zellbiologischen Nährmedien, Gewebekulturen, 3D-Gewebekulturen, Organen, Organtransplantaten, künstlich hergestellten Organen, Organen „on a chip“, Hilfsmedien, Laboratorien für die Biochemie, Molekularbiologie, Gentechnologie, Zellbiologie Mikrobiologie, Virologie, Pharmakologie, Toxikologie und Medizin sowie von Gegenständen, Möbeln, Abzügen, Kleidung, Gerätschaften, Apparaturen und Laborpersonal für und in diesen Laboratorien stammen,
• (D) zellbiologische Nährmedien, zellbiologische Nährsysteme, Hilfsmedien, Gerätschaften und Apparaturen zur Herstellung mindestens einer zellbiologischen Standardpräparation mit einer genau bekannten Dosis
  • (D1) mindestens einer Art von einzelligen Eukaryoten und/oder mindestens einer Art von mehrzelligen Eukaryoten,
  • (D2) mindestens einer Art von Wirtszellen für Viren oder
  • (D3) mindestens einer Art von Mikroorganismenpopulationen, infiziert mit einer genau bekannten Dosis an mindestens einer Art von Mollicuten,
• (E) mindestens eine Präparation einer genau bekannten Dosis mindestens eines Polyoxometallats und
• (F) mindestens eine Dosiervorrichtung für die mindestens eine Präparation einer genau bekannten Dosis mindestens eines Polyoxometallats,

umfassen und im Folgenden als »erfindungsgemäße Kits« bezeichnet werden.In addition, the object is achieved by kits for the extracellular and / or intracellular use of at least one polyoxometalate, the kits

• (A) at least one device for carrying out spectroscopic, microbiological and / or chemical measurements for the qualitative and / or quantitative detection of unicellular eukaryotes, multicellular eukaryotes, host cells of viruses, microorganism populations and mollicutes on at least one test sample, at least one with at least one species Mollicut infected test culture containing at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes, at least one kind of host cells or at least one kind of microorganism populations, is removable,
• (B) at least one device for evaluating and storing the test results measured on the at least one test sample,
• (C) at least one kind of Mollicuten free microbiological culture media, nutrient systems, auxiliary media, equipment and apparatus for producing at least one of at least one kind of Mollicuten free culture, containing
  • (C1) at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes,
  • (C2) at least one type of virus or host cell
  • (C3) at least one kind of microorganism populations, as a zero sample, wherein the eukaryotes, the host cells and viruses or the microorganism population of medicaments, cell cultures, 3D cell cultures, cell biological nutrient systems, cell culture media, tissue cultures, 3D tissue cultures, organs, organ transplants, artificially produced organs, organs "on a chip", auxiliary media and smears of laboratories for cell biological nutrient systems, cell biological nutrient media, tissue cultures, 3D tissue cultures, organs, organ transplants, artificially produced organs, organs "on a chip", auxiliary media, laboratories for the Biochemistry, molecular biology, genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology and medicine, as well as of articles, furniture, prints, clothing, implements, apparatus and laboratory personnel for and in these laboratories,
• (D) cell biological nutrient media, cell biological nutrient systems, auxiliary media, equipment and apparatus for preparing at least one standard cell biological preparation with a well-known dose
  • (D1) at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes,
  • (D2) at least one type of host cells for viruses or
  • (D3) at least one kind of microorganism population infected with a well-known dose of at least one kind of mollicut,
• (E) at least one preparation of a precisely known dose of at least one polyoxometalate and
• (F) at least one metering device for the at least one preparation of a precisely known dose of at least one polyoxometalate,

and hereinafter referred to as "kits of the invention".

• (G) an mindestens einer mollicutenfreien und/oder an mindestens einer mollicuteninfizierten Testkultur
  • (G1) mindestens einer Art von einzelligen Eukaryoten und/oder mindestens einer Art von mehrzelligen Eukaryoten,
  • (G2) mindestens einer Art von Wirtszellen für Viren oder
  • (G3) mindestens einer Art von Mikroorganismenpopulationen die Grenzkonzentration mindestens eines Polyoxometallats zur Inhibierung der Proliferation der jeweils mindestens einen Art von Eukaryoten, Wirtszellen für Viren oder Mikroorganismenpopulationen bestimmt,
• (H) mindestens eine mollicuteninfizierte Kultur
  • (H1) der mindestens einer Art von einzelligen Eukaryoten und/oder mindestens einer Art von mehrzelligen Eukaryoten,
  • (H2) der mindestens einer Art von Wirtszellen für Viren oder
  • (H3) der mindestens einer Art von Mikroorganismenpopulationen, deren Grenzkonzentration des mindestens einen Polyoxometallats zur Inhibierung der Proliferation nach dem Verfahrensschritt (G) bestimmt worden ist, mit dem mindestens einen Polyoxometallat portionsweise oder auf einmal versetzt, sodass in der mindestens einen mollicuteninfizierten Kultur höchstens die Grenzkonzentration des mindestens einen Polyoxometallats resultiert,
• (I) die nach dem Verfahrensschritt (H) resultierende mindestens eine mollicuteninfizierte Kultur während einer Inkubationzeit im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck von 0 Stunden bis auf Dauer inkubiert und nach einer Standzeit im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck von 0 Stunden bis auf Dauer mindestens einmal bestimmt, ob noch Mollicuten vorhanden sind und, sofern dies nicht der Fall ist,
• (J) mindestens einen Anteil der mindestens einen nunmehr mollicutenfreien Kultur
  • (J1) mindestens einer Art von einzelligen Eukaryoten und/oder mindestens einer Art von mehrzelligen Eukaryoten,
  • (J2) mindestens einer Art von Wirtszellen für Viren oder
  • (J3) mindestens einer Art von Mikroorganismenpopulationen auf mindestens ein mollicutenfreies Nährmedium überträgt und die jeweils mindestens eine Art von mollicutenfreien Eukaryoten, Wirtszellen und Mikroorganismenpopulationen in der resultierenden mindestens zweiten Kultur vermehrt.

Furthermore, the object has been achieved by the method (i) for decontaminating mollicutin-infected eukaryotic cultures, virus cultures and cultures of microorganism populations and (ii) for proliferation of the decontaminated, mollicutene-free cultures

• (G) at least one mollicutenfreien and / or at least one mollicuteninfiziert test culture
  • (G1) at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes,
  • (G2) at least one type of virus or host cell
  • (G3) at least one type of microorganism population determines the limiting concentration of at least one polyoxometalate for inhibiting the proliferation of the at least one type of eukaryote, host cells for viruses or microorganism populations,
• (H) at least one Mollicuteninfected culture
  • (H1) the at least one type of unicellular eukaryote and / or at least one type of multicellular eukaryote,
  • (H2) the at least one type of host cells for viruses or
  • (H3) the at least one type of microorganism populations whose limit concentration of the at least one polyoxometalate for inhibiting proliferation has been determined after process step (G), added to the at least one polyoxometallate in portions or at once, so that in the at least one mollicuteninfected culture at most Limiting concentration of the at least one polyoxometalate results,
• (I) the at least one Mollicuteninfected culture resulting after step (H) incubated for a incubation time in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure of 0 hours until permanently and after a life in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure from 0 hours to permanently at least once, determines whether there are still mollicutes and, if this is not the case,
• (J) at least a portion of the at least one now mollicutenfreien culture
  • (J1) at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes,
  • (J2) at least one type of virus or host cell
  • (J3) transmits at least one type of microorganism populations to at least one mollicin-free nutrient medium and which in each case propagates at least one species of mollicut-free eukaryotes, host cells and microorganism populations in the resulting at least second culture.

In the following, the (i) for the decontamination of mollicuteninfected eukaryotic cultures, virus cultures and cultures of microorganism populations and (ii) for the proliferation of decontaminated, mollicutenfreien cultures referred to as "inventive proliferation process".

Furthermore, the object according to the invention by the method for the decontamination and sterilization of drugs, cell biology nutrition systems, cell biology culture media and laboratories for biochemistry, molecular biology, genetic engineering, cell biology microbiology, virology, pharmacology, toxicology and medicine and of items, clothing, equipment, Furniture, fume cupboards and laboratory personnel for and in these laboratories, using at least one detergent containing at least one POM and / or at least one POM preparation in a minimum dosage> the limiting concentration.

In the following, the method for decontamination and sterilization is referred to as "sterilization method according to the invention".

• - in Form einer Säure, eines Salzes, eines Hydrats, eines Soft-Oxometallats (SOMS), einer Beschichtung und/oder eines Polyoxometallat-Nanocomposits, und/oder
• - in und/oder auf als sich dispergierenden, teilweise auflösenden, nicht auflösenden oder völlig auflösenden, kompakten und/oder porösen Partikeln, Beschichtungen, Coatings, Releasematerialien, Schwämmchen, Plättchen, Folienstückchen, Textilstückchen, Gewebestückchen, Cellulosefasern, Cellulosemikropartikeln, Cellulosenanopartikeln, Nanosomen, Mikrosomen, Liposomen, Tabletten, Glaskügelchen, Sprühgeräten, Verneblern und/oder als Beschichtung, die die Oberfläche hart und glatt und/oder diffusionsgeschlossen macht, und/oder
• - in Aerosolen, Nebeln, Hydrogelen, in Micellen, in Vesikeln, in Mikrokapseln, in Mikrosphären, in Gelen, in Cremes, Lotions, Salben und/oder Schäumen und/oder
• - in Verbindung mit funktionalen Materialien, Antibiotika, bioziden Materialien und/oder Nährmedien und/oder Hilfsmedien für die Zellbiologie und/oder
• - in Form seiner Ausgangsprodukte, die sich spontan zu dem mindestens einen Polyoxometallat zusammenfügen,

auf und/oder in die Gegenstände und/oder in die Fluide in einer an Eukaryotentestkulturen, Virustestkulturen oder Testkulturen von Mikroorganismenpopulationen ermittelbaren Grenzkonzentration eingebracht ist oder sind, wobei

• - zur Ermittlung der jeweiligen Grenzkonzentrationd das mindestens eine Polyoxometallat und/oder die mindestens eine Polyoxometallat-Präparation höchstens in einer an jeweils mindestens einer Testkultur im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck Konzentration anwendbar ist, in der
• - die Mollicuten in ihrer Proliferation inhibiert oder abgetötet sind, in der aber
• - das mindestens eine Polyoxometallat und/oder die mindestens eine Polyoxometallat-Präparation nach einer Inkubationzeit einer gegebenen Eukaryotentestkultur, einer gegebenen Virustestkultur oder einer von einer gegebenen Mikroorganismenpopulation stammenden mindestens einen Testkultur im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck von 0 Stunden bis auf Dauer und nach mindestens einer Mollicutendetektion nach einer Standzeit im Dunkeln und/oder unter Bestrahlen mit elektromagnetischer Strahlung und/oder unter Atmosphärendruck oder Überdruck von 0 Stunden bis auf Dauer die Proliferation der betreffenden Eukaryoten, der betreffenden Wirtszellen der Viren oder der betreffenden Mikroorganismen noch nicht inhibiert ist.

Not least, the object has been achieved by protected against Mollicutenbefall and / or protective objects, medical devices and fluids containing at least one polyoxometalate to be used according to the invention and / or at least one polyoxometalate preparation to be used according to the invention, wherein the at least one polyoxometalate and / or at least one polyoxometalate preparation

• in the form of an acid, a salt, a hydrate, a soft oxometalate (SOMS), a coating and / or a polyoxometalate nanocomposite, and / or
• in and / or on as dispersing, partially dissolving, non-dissolving or completely dissolving, compact and / or porous particles, coatings, coatings, release materials, sponges, platelets, foil pieces, textile pieces, fabric pieces, cellulose fibers, cellulose microparticles, cellulose nanoparticles, nanosomes, Microsomes, liposomes, tablets, glass beads, sprayers, nebulizers and / or as a coating that makes the surface hard and smooth and / or diffusion-closed, and / or
• in aerosols, mists, hydrogels, micelles, vesicles, microcapsules, microspheres, gels, creams, lotions, ointments and / or foams and / or
• - in connection with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary media for cell biology and / or
• in the form of its starting materials, which spontaneously combine to form the at least one polyoxometalate,

is introduced into and / or into the articles and / or into the fluids in a limit concentration determinable on eukaryotic test cultures, virus test cultures or test populations of microorganism populations, wherein

• in order to determine the respective limiting concentration, the at least one polyoxometalate and / or the at least one polyoxometalate preparation can be used at most in at least one test culture in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure concentration, in
• - The mollicutes are inhibited or killed in their proliferation, but in the
• the at least one polyoxometalate and / or the at least one polyoxometalate preparation after an incubation time of a given eukaryotic test culture, virus test culture or at least one test culture originating from a given microorganism population in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or Overpressure from 0 hours to permanent and after at least one Mollicutendetektion after a life in the dark and / or under irradiation with electromagnetic radiation and / or atmospheric pressure or overpressure of 0 hours to permanent proliferation of the respective eukaryotes, the respective host cells of the virus or the microorganisms in question is not yet inhibited.

In the following, the objects and fluids protected against mollicut infestation are referred to as "objects and fluids according to the invention"

Advantages of the invention

With regard to the prior art it was surprising and unforeseeable for the skilled person that the object underlying the present invention by means of the use according to the invention, the cultures according to the invention, the kits according to the invention, the proliferation method according to the invention and the sterilization method according to the invention and the inventive objects and fluids could be solved.

In particular, it was surprising that the polyoxometalates turned out to be temperature-insensitive, transportable and long-term storable substances that are specifically active against mollicuts, in particular mycoplasmas, killed or at least inhibited in their proliferation, so that at least a constant and harmless concentration of mollicutes in cultures, which contained unicellular and / or multicellular eukaryotes, could be cultured in viral cultures and in cultures of microorganism populations. The cultures contained eukaryotes, viruses and microorganism populations of drugs, cell cultures, 3D cell cultures, cell biology nutrient systems, cell biological nutrient media, tissue cultures, 3D tissue cultures, organs, organ transplants, man-made organs, on-chip organs and auxiliary media, and of Swabs from laboratories for biochemistry, virology, microbiology, pharmacology, toxicology and medicine as well as articles, clothing, utensils, furniture, prints, apparatus and laboratory personnel for and in these laboratories. The polyoxometalates did not alter the original eukaryotes, the host cells and viruses, and the microorganism populations that were studied, nor did they inhibit their proliferation or kill them, so that the results of research on the mollicutene-free, especially from mycoplasma-free cultures, scored, were valid.

Thus, not only extracellular mollicutes but also intracellular mollicutes could be killed or their proliferation inhibited by the polyoxometalates. Nor were there any treatment cycles that weakened the unicellular and multicellular eukaryotes, the host cells and the viruses as well as the microorganism populations and allowed them to die off by up to 80% or more. Of particular importance was that no more dangerous resistances formed. The temperature insensitivity, long shelf life, autoclavability and long service life of the polyoxometalates guaranteed that in the long run no loss of their activity in the cell cultures occurred. With all this, no complex cold chains for transport and storage were more necessary. Thus, the polyoxometalates to be used according to the invention act specifically against mollicutes in defined, experimentally determinable limit concentrations. in particular mycoplasmas, and were at the same time not bactericidal or bacteriostatic, virucidal or virustatic and fungicidal or fungistatic and also did not work against archaea, protozoa and microalgae, which could be components of microorganism populations.

Another particular advantage of polyoxometalates was that they did not adhere well to glass and plastics, especially neutral and anionic plastics, unless they were adequately addressed, making them particularly well-suited for laboratory work with these materials.

The thermostability of the polyoxometalates also allowed them to be autoclaved. The polyoxometalates were also light-stable and showed no time course in the UV spectrum in the irradiation with UV light. The same was true for visible light, so no precautions needed to be taken in this regard.

In order to rule out that the benefits and effects found were partly or entirely attributable to daylight, experiments were carried out in the dark and in daylight. No differences were found, and therefore the benefits and effects were due solely to the polyoxometalates.

Detailed description of the invention

The essential components of the use according to the invention and of the cultures according to the invention are heteropolyacids and isopolyacids and their isomers, defect structures and partial structures, collectively called polyoxometalates (POM), in the form of their molecules having a largest molecular diameter 5 2 nm, preferably ≦ 1.5 nm and in particular ≤ 1 nm, collectively called POM molecules and in the form of microparticles and nanoparticles of an average particle size of 1 nm to <1000 .mu.m, preferably 2 nm to 500 .mu.m, preferably 5 nm to 250 .mu.m, more preferably 5 nm to 150 .mu.m and in particular 5 nm to 100 μm. In the following, they will be referred to as "POM microparticles or POM nanoparticles", respectively.

It should be emphasized that the data ≤ 2 nm, ≤ 1.5 nm and ≤ 1 nm do not include a molecular diameter of 0 nm, but that the lower limit of the molecular diameter is equal to the largest diameter of the smallest existing POM molecule.

The average particle size of the POM microparticles and POM nanoparticles to be used according to the invention measured by transmission electron microscopy (TEM), scanning electron microscopy (SEM), scanning transmission electron microscopy (RTEM), atomic force microscopy (AFM) or scanning tunneling microscopy (TRM) can vary very widely and meet the requirements of the invention To be adjusted individually.

The POM microparticles and POM nanoparticles can have a wide variety of morphologies and geometric shapes, so that they can also be perfectly adapted to the requirements of the individual case in this respect.

Thus, they can be compact and have at least one cavity and / or a core-shell structure, wherein the core and the shell can be constructed of different materials. They may also have different geometric shapes such as spheres, ellipsoids, cubes, cuboids, pyramids, cones, cylinders, rhombuses, dodecahedra, truncated dodecahedra, icosahedra, truncated icosahedra, dumbbells, tori, platelets or needles with circular, oval, elliptical, square, triangular , quadrangular, pentagonal, hexagonal, heptagonal, octagonal, or star-shaped (tri-quad, quadrilateral, pentagonal, or polygonal) outline. If necessary, existing edges and corners can be rounded. It is also possible for two or more POM microparticles and / or POM nanoparticles of different morphology and / or geometric form to assemble together. For example, spherical POM microparticles and / or POM nanoparticles may have pointed outgrowths in the form of cones. Or, two or three cylindrical POM microparticles and / or POM nanoparticles may assemble to form a T-shaped or Y-shaped particle. Furthermore, their surface may have pits so that the POM microparticles and / or POM nanoparticles have a strawberry, raspberry or blackberry morphology. Last but not least, the dumbbells, tori, needles or plates can be bent in at least one direction of the room.

The diameter of the POM microparticles and POM nanoparticles can vary widely and therefore be perfectly adapted to the requirements of the individual case.

In the context of the present invention, the diameter of the POM microparticles and / or POM nanoparticles to be used according to the invention, which have no spherical shape, is equal to the longest distance traveled by the respective POM microparticles and POM nanoparticles.

The diameter of preferred nanoparticles according to the invention is preferably 1 nm to <1000 μm, preferably 2 nm to 500 μm, preferably 5 nm to 250 μm, particularly preferably 5 nm to 150 μm and in particular 5 nm to 100 μm.

The elemental composition and structure of the POM can also vary very widely.

• - das Lindquist-Hexamolybdatanion, Mo₆O₁₉ ^2-,
• - das Decavanadatanion, V₁₀O₂₈ ^6-,
• - das Paratungstatanion B, H₂W₁₂O₄₂ ^10-,
• - Mo36-Polymolybdate, Mo₃₆O₁₁₂(H₂O)^8-,
• - die Strandberg-Struktur, HP₂Mo₅O₂₃ ^4-,
• - die Keggin-Struktur, XM₁₂O₄₀ ^n-,
• - die Dawson-Struktur, X₂M₁₈O₆₂ ^n-,
• - die Anderson-Struktur, XM₆O₂₄ ^n-,
• - die Allman-Waugh-Struktur, X₁₂M₁₈O₃₂ ^n-,
• - die Weakley-Yamase- Struktur, XM₁₀O₃₆ ^n-, und
• - die Dexter-Silverton-Struktur, XM₁₂O₄₂ ^n-.

For example, the classification of the POM into the following structures is known:

• the Lindquist hexamolybdate anion, Mo ₆ O ₁₉ ^2- ,
• the decavanadate anion, V ₁₀ O ₂₈ ^6- ,
• the paratization stat B, H ₂ W ₁₂ O ₄₂ ^10- ,
• Mo36 polymolybddate, Mo ₃₆ O ₁₁₂ (H ₂ O) ^8- ,
• - the Strandberg structure, HP ₂ Mo ₅ O ₂₃ ^4- ,
• the Keggin structure, XM ₁₂ O ₄₀ ^n- ,
• the Dawson structure, X ₂ M ₁₈ O ₆₂ ^n- ,
• the Anderson structure, XM ₆ O ₂₄ ^n- ,
• the Allman Waugh structure, X ₁₂ M ₁₈ O ₃₂ ⁿ ,
• the Weakley-Yamase structure, XM ₁₀ O ₃₆ ⁿ , and
• - the Dexter-Silverton structure, XM ₁₂ O ₄₂ ^n- .

The n-prime number here is an integer from 3 to 20 denoting the valence of an anion that varies depending on the variables X and M.

As a further principle of order for POM the formulas I to XIII can serve: - (BW ₁₂ O ₄₀ ) ^5- (I), - (W ₁₀ O ₃₂ ) ^4- (II), - (P ₂ W ₁₈ O ₆₂ ) ^6- (III), - (PW ₁₁ O ₃₉ ) ^7- (IV), - (SiW ₁₁ O ₃₉ ) ^8- (V), - (HSiW ₉ O ₃₄ ) ^9- (VI), - (HPW ₉ O ₃₄ ) ^8- (VII), - (TM) ₄ (PW ₉ O ₃₄ ) ^t- (VIII), - (TM) ₄ (P ₂ W ₁₅ O ₅₆ ) ₂ ^t- (IX), - (NaP ₅ W ₃₀ O ₁₁₀ ) ^14- (X), - (TM) ₃ (PW ₉ O ₃₄ ) ₂ ^12- (XI) and - (P ₂ W ₁₈ O ₆ ) ^6- (XII).

In formulas I to XII, TM is a divalent or trivalent transition metal ion such as Mn ²⁺ , Fe ²⁺ , Fe ³⁺ , Co ²⁺ , Co ³⁺ , Ni ²⁺ , Cu ²⁺ and Zn ²⁺ . The superscript t is an integer and denotes the valence of an anion, which varies depending on the significance of the variable TM.

Furthermore, POM of general formula XIII come into consideration: - (A _x Ga _y Nb _a O _b ) ^z- (XIII).

In the formula XIII the variable A stands for phosphorus, silicon or germanium and the index x stands for 0 or for an integer from 1 to 40. The index y stands for an integer from 1 to 10, the index a stands for one integer from 1 to 8 and the index b is an integer from 15 to 150. The z factor varies depending on the nature and the degree of oxidation of the variable A. The aqua complexes and the active fragments of the POM XIII are also suitable.

When the index x is 0, y is preferably 6-a, where the index a is an integer of 1 to 5 and the index b is 19.

When the variable A is silicon or germanium, the index x is 2, the index y is 18, the index a is 6 and the index b is 77.

When the variable A is P, the index x is 2 or 4, the index y is 12, 15, 17 or 30, the index a is 1, 3 or 6 and the index b is 62 or 123.

In addition, the isomers of POM into consideration. Thus, the keggin structure has five isomers, the alpha, beta, gamma, delta, and epsilon structure. Furthermore, defect structures or lacunary structures as well as partial structures can be considered.

Preferably, the anions I to XIII are applied in the form of salts with cations which are approved for cleansing and personal care and pharmaceutical use.

• - H⁺, Na⁺, K⁺ und NH₄ ⁺,
• - Mono-, Di-, Tri- oder Tetra-(C₁-C₂₀-alkylammonium) wie Pentadecyldimethylferrocenylmethylammonium, Undecyldimethylferrocenylmethylammonium, Hexadecyltrimethylammonium, Octadecyltrimethylammonium, Didodecyldimethylammonium, Ditetradecyldimethylammonium, Dihexadecyldimethylammonium, Dioctadecyldimethylammonium, Dioctadecylviologen, Trioctadecylmethylammonium und Tetrabutylammonium,
• - Mono-, Di-, Tri- oder Tetra-(C₁-C₂₀-alkanolammonium) wie Ethanolammonium Diethanolammonium und Triethanolammonium
• - Monokationen natürlich vorkommender Aminosäuren wie Histidinium (HISH⁺), Argininium (ARGH⁺) oder Lysinium (LYSH⁺) oder Oligo- oder Polypeptide mit einem oder mehreren protonierten basischen Aminosäurerest(en).

Examples of suitable cations are

• H ⁺ , Na ⁺ , K ⁺ and NH ₄ ⁺ ,
• Mono-, di-, tri- or tetra- (C ₁ -C ₂₀ -alkylammonium) such as pentadecyldimethylferrocenylmethylammonium, undecyldimethylferrocenylmethylammonium, hexadecyltrimethylammonium, octadecyltrimethylammonium, didodecyldimethylammonium, ditetradecyldimethylammonium, dihexadecyldimethylammonium, dioctadecyldimethylammonium, dioctadecylviologen, trioctadecylmethylammonium and tetrabutylammonium;
• - Mono-, di-, tri- or tetra- (C ₁ -C ₂₀ alkanolammonium) such as ethanolammonium diethanolammonium and triethanolammonium
• Monocations of naturally occurring amino acids such as histidinium (HISH ⁺ ), argininium (ARGH ⁺ ) or lysinium (LYSH ⁺ ) or oligo- or polypeptides having one or more protonated basic amino acid residues.

[See. US 6,020,369 , Column 3, line 6, to column 4, line 29]

Also included are natural, modified natural and synthetic cationic oligomers and polymers, i.e., oligomers and polymers bearing primary, secondary, tertiary and quaternary ammonium groups, primary, secondary and tertiary sulfonium groups and / or primary, secondary and tertiary phosphonium groups. Synthetic oligomers and polymers are common and well known and are used, for example, in electrodeposition paints. Examples of natural cationic oligomers and polymers are polyamino-saccharides such as polyglucosamines, especially chitosan.

Examples of suitable POM are shown in Table 1. Table 1: Sum formulas of suitable POMs ^a) No. Molecular formula family structure 1 [(NMP) ₂ H] ₃ PW ₁₂ O ₄₀ 2 [(DMA) ₂ H] ₃ PMO ₁₂ O ₄₀ 3 (NH ₄ ) ₁₇ Na [NaSb ₉ W ₂₁ O ₈₆ ] Inorganic crypt 4 a- and bH ₅ BW ₁₂ O ₄₀ " 5 a- and bH ₆ ZnW ₁₂ O ₄₀ " 6 a and bH ₆ P ₂ W ₁₈ O ₆₂ " 7 alpha- (NH ₄ ) ₆ P ₂ W ₁₈ O ₆₂ Wells-Dawson structure 8th K ₁₀ Cu ₄ (H ₂ O) ₂ (PW ₉ O ₃₄ ) ₂ .20H ₂ O " 9 K ₁₀ CO ₄ (H ₂ O) ₂ (PW ₉ O ₃₄ ) ₂ .20H ₂ O " 10 Na ₇ PW ₁₁ O ₃₉ " Na ₇ PW ₁₁ O ₃₉ .20H ₂ O + 2 C ₈ H ₅ P (O) (OH) ₂ " 11 [(n-butyl) ₄ N] ₄ H ₃ PW ₁₁ O ₃₉ " 12 b-Na ₈ HPW ₉ O ₃₄ " 13 [(n-butyl) ₄ N] ₃ PMoW ₁₁ O ₃₉ " 14 a - [(n-butyl) ₄ N] ₄ Mo8O26 " 15 [(n-butyl) ₄ N] ₂ W ₆ O ₁₉ " 16 [(n-butyl) _a N] ₂ Mo ₆ O ₁₉ " 17 a- (NH ₄ ) _n H _(4-n) SiW ₁₂ O ₄₀ " 18 a- (NH ₄ ) _n H _(5-n) BW ₁₂ O ₄₀ " 19 AK ₅ BW ₁₂ O ₄₀ " 20 K ₄ W ₄ O ₁₀ (O ₂ ) ₆ , " 21 b-Na ₉ HSiW ₉ O ₃₄ " 22 Na ₆ H ₂ W ₁₂ O ₄₀ 23 (NH ₄ ) ₁₄ [NaP ₅ W ₃₀ O ₁₁₀ ] Preyssler structure 24 a- (NH ₄ ) ₅ BW ₁₂ O ₄₀ " 25 a-Na ₅ BW ₁₂ O ₄₀ " 26 (NH ₄ ) ₄ W ₁₀ O ₃₂ " "27 (Me ₄ N) ₄ W ₁₀ O ₃₂ " 28 (HISH ⁺ ) _n H _(5-n) BW ₁₂ O ₄₀ " 29 (LYSH ⁺ ) _n H _(5-n) BW ₁₂ O ₄₀ " 30 (ARGH ⁺ ) _n H _(5-n) BW ₁₂ O ₄₀ " 31 (HISH ⁺ ) _n H _(4-1) SiW ₁₂ O ₄₀ " 32 (LYSH ⁺ ) _n H _(4-n) SiW ₁₂ O ₄₀ " 34 (ARGH ⁺ ) _n H _(4-n) SiW ₁₂ O ₄₀ " 35 K ₁₂ [EuP ₅ W ₃₀ O ₁₁₀ ] .22H ₂ O ^b) n 36 AK ₈ SiW ₁₁ O ₃₉ " 37 K ₁₀ (H ₂ W ₁₂ O ₄₂ ) " 38 K ₁₂ Ni ₃ (II) (PW ₉ O ₃₄ ) ₂ .nH ₂ O " 39 (NH ₄ ) ₁₀ Co ₄ (II) (PW ₉ O ₃₄ ) ₂ .nH ₂ O " 40 K ₁₂ Pd ₃ (II) (PW ₉ O ₃₄ ) ₂ .nH ₂ O " 41 Na ₁₂ P ₂ W ₁₅ O ₅₆ -18H ₂ O Lacunare (defective) structure 42 Na ₁₆ Cu ₄ (H ₂ O) ₂ (P ₂ W ₁₅ O ₅₆ ) ₂ .nH ₂ O " 43 Na ₁₆ Zn ₄ (H ₂ O) ₂ (P ₂ W ₁₅ O ₅₆ ) ₂ .nH ₂ O " 44 Na ₁₆ CO ₄ (H ₂ O) ₂ (P ₂ W ₁₅ O ₅₆ ) ₂ . nH ₂ O " 45 Na ₁₆ N ₁₄ (H ₂ O) ₂ (P ₂ W ₁₅ O ₅₆ ) ₂ .nH ₂ O Wells-Dawson sandwich structure 46 Na ₁₆ Mn ₄ (H ₂ O) ₂ (P ₂ W ₁₅ O ₅₆ ) ₂ .nH ₂ O " 47 Na ₁₆ Fe ₄ (H ₂ O) ₂ (P ₂ W ₁₅ O ₅₆ ) ₂ .nH ₂ O " 48 K ₁₀ Zn ₄ (H ₂ O) ₂ (PW ₉ O ₃₄ ) ₂ .20H ₂ O Keggin sandwich structure 49 K ₁₀ Ni ₄ (H ₂ O) ₂ (PW ₉ O ₃₄ ) ₂ .nH ₂ O " 50 K ₁₀ Mn ₄ (H ₂ O) ₂ (PW ₉ O ₃₄ ) ₂ .nH ₂ O " 51 K ₁₀ Fe ₄ (H ₂ O) ₂ (PW ₉ O ₃₄ ) ₂ .nH ₂ O " 52 K ₁₂ Cu ₃ (PW ₉ O ₃₄ ) ₂ .nH ₂ O " 53 K ₁₂ (CoH ₂ O) ₃ (PW ₉ O ₃₄ ) ₂ .nH ₂ O " 54 K ₁₂ Zn ₃ (PN ₉ O ₃₄ ) ₂ .15H ₂ O " 55 K ₁₂ Mn ₃ (PW ₉ O ₃₄ ) ₂ .15H ₂ O " 56 K ₁₂ Fe ₃ (PW ₉ O ₃₄ ) ₂ .25H ₂ O " 57 (ARGH ⁺ ) ₁₀ (NH ₄ ) ₇ Na [NaSb ₉ W ₂₁ O ₈₆ ] " 58 (ARGH ⁺ ) ₅ HW ₁₁ O ₃₉ .17H ₂ O " 59 K ₇ Ti ₂ W ₁₀ O ₄₀ " 60 [(CH ₃ ) ₄ N] ₇ Ti ₂ W ₁₀ O ₄₀ " 61 Cs ₇ Ti ₂ W ₁₀ O ₄₀ " 62 [HISH ⁺ ] ₇ Ti ₂ W ₁₀ O ₄₀ " 63 [LYSH ⁺ ] _n Na _7-n PTi ₂ W ₁₀ O ₄₀ " 64 [ARGH ⁺ ] _n Na _7-n PTi ₂ W ₁₀ O ₄₀ " 65 [n-butyl ₄ N ⁺ ] ₃ H ₃ V ₁₀ O ₂₈ " 66 K ₇ HNb ₆ O ₁₉ .13H2O " 67 [(CH ₃ ) ₄ N ⁺ ] ₄ SiW ₁₁ O ₃₉ -O [SiCH ₂ CH ₂ C (O) OCH ₃ ] ₂ Organically modified structure 68 [(CH ₃ ) ₄ N ⁺ ] ₄ PW ₁₁ O ₃₉ - (SiCH ₂ CH ₂ CH ₂ CN) " 69 [(CH ₃ ) ₄ N ⁺ ] ₄ PW ₁₁ O ₃₉ - (SiCH ₂ CH ₂ CH ₂ Cl) " 70 [(CH ₃ ) ₄ N ⁺ ] ₄ PW ₁₁ O ₃₉ - (SiCH ₂ = CH ₂ ) " 71 Cs ₄ [SiW11O ₃₉ - (CH ₂ CH ₂ C (O) OCH _{3) 2] 4} " 72 Cs ₄ [SiW11O ₃₉ - (SiCH ₂ CH ₂ CH ₂ CN)] ₄ " 73 Cs ₄ [SiW11O ₃₉ - (CH ₂ CH ₂ CH ₂ Cl) _{2] 4} " 74 Cs ₄ [SiW11O ₃₉ - (SiCH ₂ = CH ₂ )] ₄ " 75 [(CH ₃ ) _a N ⁺ ] _a SiW ₁₁ O ₃₉ -O- (SiCH ₂ CH ₂ CH ₂ Cl) ₂ " 76 [(CH ₃ ) ₄ N ⁺ ] ₄ SiW ₁₁ O ₃₉ -O (SiCH ₂ CH ₂ CH ₂ CN) ₂ " 77 [(CH ₃ ) ₄ N ⁺ ] ₄ SiW ₁₁ O ₃₉ -O (SiCH ₂ = CH ₂ ) ₂ " 78 [(CH ₃ ) ₄ N ⁺ ] ₄ SiW ₁₁ O ₃₉ -O [SiC (CH ₃ )] ₂ " 79 [(CH ₃ ) ₄ N ⁺ ] ₄ SiW ₁₁ O ₃₉ -O [SiCH ₂ CH (CH ₃ )] ₂ " 80 [(CH ₃ ) ₄ N ⁺ ] ₄ SiW ₁₁ O ₃₉ -O [SiCH ₂ CH ₂ C (O) OCH ₃ ] ₂ " 81 K ₅ Mn (II) PW ₁₁ O ₃₉ .nH ₂ O Structure substituted with transition metals 82 K ₈ Mn (II) P ₂ W ₁₇ O ₆₁ .nH ₂ O " 83 K ₆ Mn (II) SiW ₁₁ O ₃₉ .nH ₂ O " 84 K ₅ PW ₁₁ O ₃₉ [Si (CH ₃ ) ₂ ] .nH ₂ O " 85 K ₃ PW ₁₁ O ₄₁ (PC ₆ H ₅ ) ₂ .nH ₂ O " 86 Na ₃ PW ₁₁ O ₄₁ (PC ₆ H ₅ ) ₂ .nH ₂ O " 87 K ₅ PTiW ₁₁ O ₄₀ " 88 Cs ₅ PTiW ₁₁ O ₃₉ " 89 K ₆ SiW ₁₁ O ₃₉ [Si (CH ₃ ) ₂ ] .nH ₂ O " 90 KSiW ₁₁ O ₃₉ [Si (C ₆ H ₅ ) (tert-C ₄ H ₉ )]. NH ₂ O " 91 K ₆ SiW ₁₁ O ₃₉ [Si (C ₆ H ₅ ) ₂ ] .nH ₂ O " 92 K ₇ SiW ₉ Nb ₃ O ₄₀ .nH ₂ O " 93 Cs ₇ SiW ₉ Nb ₃ O ₄₀ .nH ₂ O " 94 Cs ₈ Si ₂ W ₁₈ Nb ₆ O ₇₇ .nH ₂ O " 95 [(CH ₃ ) ₃ NH ⁺ ] ₇ SiW ₉ Nb ₃ O ₄₀ .nH ₂ O Substituted Keggin structure 96 (CN ₃ H ₆ ) ₇ SiW ₉ Nb ₃ O ₄₀ .nH ₂ O " 97 (CN ₃ H ₆ ) ₈ Si ₂ W ₁₈ Nb ₆ O ₇₇ .nH ₂ O " 98 Rb ₇ SiW ₉ Nb ₃ O ₄₀ .nH ₂ O " 99 Rb ₈ Si ₂ W ₁₈ Nb ₆ O ₇₇ .nH ₂ O " 100 K ₈ Si ₂ W ₁₈ Nb ₆ O ₇₇ .nH ₂ O " 101 K ₆ P ₂ Mo ₁₈ O ₆₂ .nH ₂ O " 102 (C ₅ H ₅ N) ₇ HSi ₂ W ₁₈ Nb ₆ O ₇₇ .nH ₂ O " 103 (C ₅ H ₅ N) ₇ SiW ₉ Nb ₃ O ₄₀ .nH ₂ O " 104 (ARGH ⁺ ) ₈ SiW ₁₈ Nb ₆ O ₇₇ .18H ₂ O " 105 (LYSH ⁺ ) ₇ KSiW ₁₈ Nb ₆ O ₇₇ .18H ₂ O " 106 (HISH ⁺ ) ₆ K ₂ SiW ₁₈ Nb ₆ O ₇₇ .18H ₂ O " 107 [(CH ₃ ) ₄ N ⁺ ] ₄ SiW ₁₁ O ₃₉ -O (SiCH ₂ CH ₃ ) ₂ " 108 [(CH ₃ ) ₄ N ⁺ ] ₄ SiW ₁₁ O ₃₉ -O (SiCH ₃ ) ₂ " 109 [(CH ₃ ) ₄ N ⁺ ] ₄ SiW ₁₁ O ₃₉ -O (SiC ₁₆ H ₃₃ ) ₂ " 110 Li ₉ P ₂ V ₃ (CH ₃ ) ₃ W ₁₂ O ₆₂ " 111 Li ₇ HSi ₂ W ₁₈ Nb ₆ O ₇₇ " 112 Cs ₉ P ₂ V ₃ CH ₃ W ₁₂ O ₆₂ " 113 Cs ₁₂ P ₂ V ₃ W ₁₂ O ₆₂ " 114 K ₄ H ₂ PV ₄ W ₈ O ₄₀ " 115 Na ₁₂ P ₄ W ₁₄ O ₅₈ " 116 Na ₁₄ H ₆ P ₆ W ₁₈ O ₇₉ " 117 aK ₅ (NbO ₂ ) SiW ₁₁ O ₃₉ " 118 (aO ₂ ) SiW ₁₁ O ₃₉ " 119 [(CH ₃ ) ₃ NH ⁺ ) ₅ NbSiW ₁₁ O ₄₀ " 120 [(CH ₃ ) ₃ NH ⁺ ] ₅ TaSiW ₁₁ O ₄₀ " 121 K ₆ Nb ₃ PW ₉ O ₄₀ Peroxo Keggin structure 122 [(CH ₃ ) ₃ NH ⁺ ] ₅ (NbO ₂ ) SiW ₁₁ O ₃₉ " 123 [(CH ₃ ) ₃ NH ⁺ ] ₅ (TaO ₂ ) SiW ₁₁ O ₃₉ " 124 K ₄ (NbO ₂ ) PW ₁₁ O ₃₉ " 125 K ₇ (NbO ₂ ) P ₂ W ₁₂ O ₆₁ " 126 [(CH ₃ ) ₃ NH ⁺ ] ₇ (NbO ₂ ) ₃ SiW ₉ O ₃₇ " 127 Cs ₇ (NbO ₂ ) ₃ SiW ₉ O ₃₇ " 128 K ₆ (NbO ₂ ) ₃ PW ₉ O ₃₇ " 129 Na ₁₀ (H ₂ W ₁₂ O ₄₂ ) " 130 K ₄ NbPW ₁₁ O ₄₀ " 131 [(CH ₃ ) ₃ NH ₊ ] ₄ NbPW ₁₁ O ₄₀ " 132 K ₅ NbSiW ₁₁ O ₄₀ " 133 K ₅ TaSiW ₁₁ O ₄₀ " 134 K ₇ NbP ₂ W ₁₇ O ₆₂ Wells-Dawson structure 135 K ₇ (TiO ₂ ) ₂ PW ₁₀ O ₃₈ " 136 K ₇ (TaO ₂ ) ₃ SiW ₉ O ₃₇ " 137 K ₇ Ta ₃ SiW ₉ O ₄₀ " 138 K ₆ (TaO ₂ ) ₃ PW ₉ O ₃₇ " 139 K ₆ Ta ₃ PW ₉ O ₄₀ " 140 K ₈ CO ₂ W ₁₁ O ₃₉ " 141 H ₂ [(CH ₃ ) ₄ N ⁺ ] 4 (C ₂ H ₅ Si) ₂ CoW ₁₁ O ₄₀ " 142 H ₂ [(CH ₃ ) ₄ N ⁺ ] ₄ (iso-C ₄ H ₉ Si) ₂ CoW ₁₁ O ₄₀ " 143 K ₉ Nb ₃ P ₂ W ₁₅ O ₆₂ " 144 K ₉ (NbO ₂ ) ₃ P ₂ W ₁₅ O ₅₉ " 145 K ₁₂ (NbO ₂ ) ₆ P ₂ W ₁₂ O ₅₆ Well-Dawson peroxo 146 K1 ₂ Nb ₆ P ₂ W ₁₂ O ₆₂ Wells-Dawson structure ff. 147 a ₂ -K ₁₀ P ₂ W ₁₇ O ₆₁ " 148 K _e Fe (III) Nb ₃ P ₂ W ₁₅ O ₆₂ " 149 K ₇ Zn (II) Nb ₃ P ₂ W ₁₅ O ₆₂ " 150 (NH ₄ ) ₆ (aP ₂ W ₁₈ O ₆₂ ) .nH ₂ O " 151 K ₁₂ [H ₂ P ₂ W ₁₂ O ₄₈ ] .24H ₂ O " 152 K ₂ Na ₁₅ H ₅ [PtMo ₆ O ₂₄ ] .8H ₂ O " 153 K ₈ [a ₂ -P ₂ W ₁₇ MoO ₆₂ ] .nH ₂ O " 154 KHP ₂ V ₃ W ₁₅ O ₆₂ .34H ₂ O " 155 K ₆ [P ₂ W ₁₂ Nb ₆ O ₆₂ ] .24H ₂ O " 156 Na ₆ [V ₁₀ O ₂₈ ] .H ₂ O " 157 (Guanidinium) ₈ H [PV ₁₄ O ₆₂ ] .3H ₂ O " 158 K8H [PV14O62] " 159 Na ₇ [MnV ₁₃ O ₃₈ ] .18H ₂ O " 160 K ₆ [BW ₁₁ O ₃₉ Ga (OH) ₂ ] .13H ₂ O " 161 K ₇ H [Nb ₆ O ₁₉ ] .13H ₂ O " 162 [(CH ₃ ) ₄ N ⁺ / Na ⁺ / K ⁺ ] ₄ [Nb ₂ W ₄ O ₁₉ ] " 163 [(CH ₃ ) ₄ N ⁺ ] ₉ [P ₂ W ₁₅ Nb ₃ O ₆₂ ] " 164 [(CH ₃ ) ₄ N ⁺ ] ₁₅ [HP ₄ W ₃₀ Nb ₆ O ₁₂₃ ] .16H ₂ O " 165 [Na / K] ₆ [Nb ₄ W ₂ O ₁₉ ] " 166 [(CH _{3) 4} N ⁺ / Na ⁺ / K ^+] 5 _[Nb3W3O19]. 6H ₂ O " 167 K ₅ [CpTiSiW ₁₁ O ₃₉ ] .12H ₂ O " 169 b ₂ -K ₈ [SiWnO ₃₉ ] .14H ₂ O " 170 aK ₈ [SiW ₁₀ O ₃₆ ] .12H ₂ O " 171 Cs ₇ Na ₂ [PW ₁₀ O ₃₇ ] .8H ₂ O " 172 Cs ₆ [P ₂ W ₅ O ₂₃ ] .7.5H ₂ O " 173 g-Cs ₇ [PW ₁₀ O ₃₆ ] .7H ₂ O " 174 K ₅ [SiNbW ₁₁ O ₄₀ ] .7H ₂ O " 175 K ₄ [PNbW ₁₁ O ₄₀ ] .12H ₂ O " 176 Na ₆ [Nb ₄ W ₂ O ₁₉ ] .13H ₂ O " 177 K ₆ [Nb ₄ W ₂ O ₁₉ ] .7H ₂ O " 180 K ₄ [V ₂ W ₄ O ₁₉ ] .3.5H ₂ O " 181 Na ₅ [V ₃ W ₃ O ₁₉ ] .12H ₂ O " 182 K ₆ [PV ₃ W ₉ O ₄₀ ] .14H ₂ O " 183 Na ₉ [Ab-GeW ₉ O ₃₄ ] .8H ₂ O " 184 Na ₁₀ [Aa-GeW ₉ O ₃₄ ] .9H ₂ O " 185 K ₇ [BV ₂ W ₁₀ O ₄₀ ] .6H ₂ O " 186 Na ₅ [CH ₃ Sn (Nb ₆ O ₁₉ )]. 10H ₂ O " 187 Na ₈ [Pt (P (m-SO ₃ C ₆ H ₅ ) ₃ ) ₃ Cl] .3H ₂ O " 188 [(CH ₃ ) ₃ NH ⁺ ] ₁₀ (H) [Si (H) ₃ W ₁₈ O ₆₈ ] .10H ₂ O " 189 K ₇ [Aa-GeNb ₃ W ₉ O ₄₀ ]. 18H ₂ O " 190 K ₇ [Ab-SiNb ₃ W ₉ O ₄₀ ] .20H ₂ O " 191 [(CH ₃ ) ₃ NH ⁺ ] ₉ [Aa-HGe ₂ Nb ₆ W ₁₈ O ₇₈ " 192 K ₇ (H) [Aa-Ge ₂ Nb ₆ W ₁₈ O ₇₇ ] .18H ₂ O " 193 K ₈ [Ab-Si ₂ Nb ₆ W ₁₈ O ₇₇ ] " 194 [(CH ₃ ) ₃ NH ⁺ ] ₈ [AB-Si ₂ Nb ₆ W ₁₈ O ₇₇ ] " a) see. US 6,020,369 , TABLE 1, columns 3 to 10; b) Tierui Zhang, Shaoquin Liu, Dirk G. Kurth, and Charl FJ Faul, Organized Nanostructured Complexes of Polyoxometalates and Surfactants that Exhibit Photoluminescence and Electrochromism, Advanced Functional Materials, 2009, 19, 642-652; n Number, in particular integer, from 1 to 50.

Further examples of suitable POM are from the American patent US Pat. No. 7,097,858 B2 , Column 14, line 56, to column 17, line 19, and from TABLE 8a, column 22, line 41, to column 23, line 28, compounds number 1-53, and TABLE 8b, column 23, line 30, to column 25, line 34, compounds number 1 to 150, known.

Other preferred polyoxometalates are those of JTRhule, CL Hill and DA Judd in the article "Polyoxometalates in Medicine" in Chemical Reviews, Vol. 98, pages 327 to 357, in Table 1 "In Vitro Antiviral Activities of Polyoxometalates," pages 332 to 347 , and in Table 2, "In Vivo Activities of Polyoxometalates," page 351, listed polyoxometalates that have been tested for antiviral activity.

• - Ammoniumheptamolybdat-Tetrahydrat {(NH₄)₆Mo₇O₂₄] · 4H₂O, CAS-Nr. 13106-76-8 (wasserfrei), CAS-Nr. 12054-85-2 (Tetrahydrat), AHMT},
• - Wolframatophosphorsäure-Hydrat {H₃[P(W₃O₁₀)₄] ·xH₂O, CAS-Nr. 1343-93-7 (wasserfrei), CAS-Nr. 12067-99-1 (Hydrat), Wo-Pho},
• - Molybdatophosphorsäure-Hydrat, {H₃P(Mo₃O₁₀)₄]· xH2O, CAS-Nr.:12026-57-2 (wasserfrei), CAS-Nr. 51429-74-4 (Hydrat), Mo-Pho} und/oder
• - Wolframatokieselsäure {H₄[Si(W₃O₁₀)₄] · xH₂O, CAS-Nr. 12027-43-9, CAS-Nr. WKS}

und/oder ihre Salze verwendet. Very particularly preferred

• - Ammonium heptamolybdate tetrahydrate {(NH ₄ ) ₆ Mo ₇ O ₂₄ ] · 4H ₂ O, CAS no. 13106-76-8 (anhydrous), CAS no. 12054-85-2 (tetrahydrate), AHMT},
• Tungstophosphoric hydrate {H ₃ [P (W ₃ O ₁₀ ) ₄ ] .xH ₂ O, CAS-No. 1343-93-7 (anhydrous), CAS no. 12067-99-1 (hydrate), Wo-Pho},
• - Molybdatophosphoric hydrate, {H ₃ P (Mo ₃ O ₁₀ ) ₄ ] · xH ₂ O, CAS No.:12026-57-2 (anhydrous), CAS-No. 51429-74-4 (hydrate), Mo-Pho} and / or
• Tungstosilicic acid {H ₄ [Si (W ₃ O ₁₀ ) ₄ ] .xH ₂ O, CAS no. 12027-43-9, CAS no. WKS}

and / or their salts.

The POM microparticles and POM nanoparticles to be used according to the invention can be prepared by means of customary and known wet-chemical processes, such as, for example, precipitation processes. However, it is also possible to dissolve the POM in water and to spray the resulting solution against a warm stream of air. In addition, it is possible to evaporate the solution under vacuum, being irradiated with IR radiation. Furthermore, it is possible to prepare solutions, in particular aqueous, solutions of POM on cold surfaces, such as frozen, smooth metal surfaces, dry ice, cryogenic organic solvents and liquefied gases, such as methane, ethane propane, butane, methylcyclohexane or benzine, liquid nitrogen or liquid helium spray on and evaporate the dry ice or the liquid substances.

The molecular POM, POM microparticles and / or POM nanoparticles described above are unchanged, oxygen-substituted, functionalized, aggregated, agglomerated and / or supported. For example, they may be functionalized, agglomerated and supported. But they can also not be functionalized and not aggregated.

• - in Form einer Säure, eines Salzes, eines Hydrats, eines Soft-Oxometallats (SOMS), einer Beschichtung und/oder eines Polyoxometallat-Nanocomposits, und/oder
• - in und/oder auf als sich dispergierenden, teilweise auflösenden, nicht auflösenden oder völlig auflösenden, kompakten und/oder porösen Partikeln, Beschichtungen, Coatings, Releasematerialien, Schwämmchen, Plättchen, Folienstückchen, Textilstückchen, Gewebestückchen, Cellulosefasern, Cellulosemikropartikeln, Cellulosenanopartikeln, Nanosomen, Mikrosomen, Liposomen, Tabletten, Glaskügelchen, Sprühgeräten, Verneblern und/oder als Beschichtung, die die Oberfläche hart und glatt und/oder diffusionsgeschlossen macht, und/oder
• - in Aerosolen, Nebeln, Hydrogelen, in Micellen, in Vesikeln, in Mikrokapseln, in Mikrosphären, in Gelen, in Cremes, Lotions, Salben und/oder Schäumen und/oder
• - in Verbindung mit funktionalen Materialien, Antibiotika, bioziden Materialien und/oder Nährmedien und/oder Hilfsmedien für die Zellbiologie und/oder
• - in Form ihrer Ausgangsprodukte, die sich spontan zu dem mindestens einen Polyoxometallat zusammenfügen,

vorliegen.Furthermore, they can

• in the form of an acid, a salt, a hydrate, a soft oxometalate (SOMS), a coating and / or a polyoxometalate nanocomposite, and / or
• in and / or on as dispersing, partially dissolving, non-dissolving or completely dissolving, compact and / or porous particles, coatings, coatings, release materials, sponges, platelets, foil pieces, textile pieces, fabric pieces, cellulose fibers, cellulose microparticles, cellulose nanoparticles, nanosomes, Microsomes, liposomes, tablets, glass beads, sprayers, nebulizers and / or as a coating that makes the surface hard and smooth and / or diffusion-closed, and / or
• in aerosols, mists, hydrogels, micelles, vesicles, microcapsules, microspheres, gels, creams, lotions, ointments and / or foams and / or
• - in conjunction with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary media for cell biology and / or
• in the form of their starting materials, which spontaneously combine to form the at least one polyoxometalate,

available.

In addition, they may be present in the POM preparations as disclosed in the documents described above.

These POM preparations can not be sterilized, pre-sterilized or ready to use. They may be pre-dispensed in pre-dosed quantities in sealed ampoules, dispensers and dispensers for solutions, suspensions, powders, creams, lotions, ointments and / or foams, disposable syringes, rupturable bags or dropper bottles.

The particles, aerosols, mist, sprayers, nebulizers, hydrogels, gels, creams, lotions, ointments and / or foams may be passable to decontaminate the laboratory staff of mollicutes without risk of damaging cultures. Care must be taken that the concentrations of the POM and / or the POM preparations do not exceed the limit concentrations defined below according to the invention.

In general, when selecting functional materials for use with the POMs, attention must be paid to the toxicity of the materials for the unicellular and multicellular eukaryotes, host cells and viruses and microorganism populations. Thus, as such or in conjunction with the POM, these functional materials may not be toxic to the unicellular and multicellular eukaryotes, host cells and viruses and microorganism populations of the cultures to be studied. But if you want to achieve special effects, such as a particularly specific and targeted effect, the functional materials can be as such or in conjunction with the POM also toxic.

One skilled in the art, a broadly skilled chemical chemist trained in cell biology with many years of experimental experience, can therefore select the appropriate functional materials from his general knowledge.

The unaltered POMs are the POMs that arise during their manufacture without further functionalization or modification.

In the oxygen-displaced POM, terminal oxide centers are replaced by other ligands, such as sulfide ions, bromide ions, amino groups, nitrosyl groups, and / or alkoxy groups. The sulfur-containing POM are also called polyoxothiometalates.

The aggregates are loose aggregates of particles that are held together by cohesion and can not be distributed by conventional and known dispersion methods. Their inner surface is smaller than the sum of the surfaces of the primary particles.

The agglomerates are aggregates of primary particles and their aggregates, which are bridged over edges and corners. Their inner surface corresponds approximately to the sum of the surfaces of the primary particles.

The molecular POM, POM microparticles and POM nanoparticles to be used according to the invention can be present "naked". That is, their surface is not surrounded by a shell and / or is not functionalized.

Furthermore, the molecular POM, POM microparticles and POM nanoparticles to be used according to the invention can be surrounded by a shell and / or carry at least one functional group. In this case, the material of the shells may carry the functional groups or else the functional groups may be present directly on the surface of the molecular POM, the POM microparticles and the POM nanoparticles.

The material of the shell and / or the functional groups can be selected so that the molecular POM, POM microparticles and POM nanoparticles to be used according to the invention are particularly rapidly and homogeneously in an organic and / or inorganic, solid or liquid matrix, in particular a distribute and / or modify the physical and / or chemical properties of the molecular POM, POM microparticles, and POM nanoparticles in a particular desired manner, and / or modify the physical and / or chemical properties of the organic and / or inorganic polymeric matrix or inorganic ceramic matrix acting as a support material and / or binder mask.

The shells and / or the functional groups may be attached to the surface of the molecular POM, POM microparticles and POM nanoparticles via covalent and / or ionic bonds and / or electrostatic and / or van der Waals forces.

The bond between the surface of the molecular POM, POM microparticles and POM nanoparticles and the shell and / or the functional groups can be permanently or reversibly, i. be solvable again.

The shells may be constructed of organic, inorganic and organometallic, polymeric, oligomeric and low molecular weight materials or combinations of at least two of these materials.

The following are examples of suitable functional groups and functional materials for the shells and / or matrices of the molecular POM, POM microparticles and POM nanoparticles to be used according to the invention. The person skilled in the art can select the functional groups and materials which are particularly suitable for the particular case on the basis of the property profiles known to him.

Common and known functional groups:

Fluorine, chlorine, bromine and iodine atoms; Hydroxyl, thiol, ether, thioether, amino, peroxide, aldehyde, acetal, carboxyl, peroxycarboxyl, ester, amide, hydrazide and urethane groups; Imide, hydrazone and hydroxime, amide and hydroxamic acid groups; Groups derived from formamidine, formamidoxime, formamidrazone, formhydrazidine, formhydrazidoxime, formamidrazone, formoxamidine, formhydroxamoxime and formoxamidrazone; Nitrile, isocyanate, thiocyanate, isothiocyanate, isonitrile, lactide, lactone, lactam, oxime, nitroso, nitro, azo, azoxy, hydrazine, hydrazone, azine, carbodiimide , Azide, azane, sulfen, sulfenamide, sulfonamide, thioaldehyde, thioketone, thioacetal, thiocarboxylic acid, sulfonium, sulfur halide, sulfoxide, sulfone, sulfimine, sulfoximine, sultone, sultam, , Sulfonic, silane, siloxane, phosphine, phosphine, phosphonium, phosphoric, phosphorous, phosphonic, phosphate, phosphinate and phosphonate groups.

Usual and Known Functional Additives for Plastics:

Examples of suitable additives are thermally and / or actinic-curable reactive diluents, low-boiling organic solvents and high-boiling organic solvents ("long solvents"), water, UV absorbers, light stabilizers, free-radical scavengers, thermolabile free-radical initiators, photoinitiators and co-initiators, crosslinking agents, as used in one-component systems, catalysts for thermal crosslinking, deaerating agents, slip additives, polymerization inhibitors, defoamers, emulsifiers, wetting and dispersing agents and surfactants, adhesion promoters, leveling agents, film-forming auxiliaries, sag control agents (SCA), rheology control additives (thickeners), Flame retardants, siccatives, drying agents, anti-skinning agents, corrosion inhibitors, waxes, matting agents, reinforcing fibers or precursors of organically modified ceramic materials.

Examples of suitable thermally curable reactive diluents are positionally isomeric diethyloctanediols or hydroxyl-containing hyperbranched compounds or dendrimers, as described, for example, in German patent applications DE 198 05 421 A 1, DE 198 09 643 A 1 or DE 198 40 405 A 1 will be described.

Examples of suitable reactive diluents curable with actinic radiation are those described in Rompp Lexikon Lacke und Druckfarben, Georg Thieme Verlag, Stuttgart, New York, 1998, on page 491 under the heading "reactive diluents". In the context of the present invention, actinic radiation is understood as meaning corpuscular radiation such as electron radiation, alpha radiation, beta radiation and proton radiation, as well as electromagnetic radiation such as infrared, visible light, UV radiation, X-radiation and gamma radiation. In particular, UV radiation is used.

Examples of suitable low-boiling organic solvents and high-boiling organic solvents ("long solvents") are ketones such as methyl ethyl ketone, methyl isoamyl ketone or methyl isobutyl ketone, esters such as ethyl acetate, butyl acetate, ethyl ethoxypropionate, methoxypropyl acetate or butyl glycol acetate ethers such as dibutyl ether or ethylene glycol, diethylene glycol, propylene glycol, dipropylene glycol , Butylene glycol or Dibutylenglykoldimethyl-, diethyl or -dibutylether, N-methylpyrrolidone or xylenes or mixtures of aromatic and / or aliphatic hydrocarbons such as Solventnaphtha®, gasoline 135/180, Dipentene or Solvesso®.

Examples of suitable thermolabile radical initiators are organic peroxides, organic azo compounds or C-C-cleaving initiators such as dialkyl peroxides, peroxycarboxylic acids, peroxodicarbonates, peroxide esters, hydroperoxides, ketone peroxides, azodinitriles or benzpinacol silyl ethers.

Examples of suitable catalysts for the crosslinking are dibutyltin dilaurate, dibutyltin dioleate, lithium decanoate, zinc octoate or bismuth salts, such as bismuth lactate or dimethylolpropionate.

Examples of suitable photoinitiators and coinitiators are described in Rompp Lexikon Lacke and Druckfarben, Georg Thieme Verlag Stuttgart, 1998, pages 444 to 446.

Examples of suitable additional crosslinking agents, as used in so-called one-component systems, are aminoplast resins, as described, for example, in Rompp Lexikon Lacke und Druckfarben, Georg Thieme Verlag, 1998, page 29, "Aminoharze", the textbook "Lackadditive" by Johan Bieleman, Wiley- VCH, Weinheim, New York, 1998, pages 242 et seq., The book "Paints, Coatings and Solvents", secondly revised edition, Edit. D. Stoye and W. Freitag, Wiley-VCH, Weinheim, New York, 1998, p. 80 et seq., Patents US 4,710,542 A 1 or EP-B-0 245 700 A 1 as well as in the article by B. Singh and Coworkers "Carbamylmethylated Melamines, Novel Crosslinkers for the Coatings Industry", in Advanced Organic Coatings Science and Technology Series, 1991, Vol. 13, pp. 193 to 207, compounds containing carboxyl groups or resins are described, for example, in the patent DE 196 52 813 A 1, epoxy group-containing compounds or resins, as described for example in the patents EP 0 299 420 A 1, DE 22 14 650 B 1, DE 27 49 576 B 1, US 4,091,048 A or US 3,781,379 A be blocked polyisocyanates, as described for example in the patents US 4,444,954 A . DE 196 17 086 A 1, DE 196 31 269 A 1, EP 0 004 571 A 1 or EP 0 582 051 A 1, and / or tris (alkoxycarbonylamino) triazines, as described in the patents US 4,939,213 A . US 5,084,541 A . US 5,288,865 A or EP 0 604 922 A 1 will be described.

Examples of suitable deaerating agents are diazadicycloundecane or benzoin.

Examples of suitable emulsifiers, wetting and dispersing agents or surfactants are the customary and known anionic, cationic, nonionic and zwitterionic wetting agents, as described in detail in, for example, Rompp Online, April 2014, Georg Thieme Verlag, "wetting agents".

An example of a suitable coupling agent is tricyclodecanedimethanol.

Examples of suitable film-forming auxiliaries are cellulose derivatives such as cellulose acetobutyrate (CAB).

Examples of suitable transparent fillers are those based on silica, alumina or zirconia; in addition, reference is made to the Rompp Lexikon Lacke and printing inks, Georg Thieme Verlag, Stuttgart, 1998, pages 250 to 252.

Examples of suitable Sag control agents are ureas, modified ureas and / or silicas, as described for example in the literature EP 0 192 304 A 1, DE 23 59 923 A 1, DE 18 05 693 A 1, WO 94/22968 . DE 27 51 761 C 1, WO 97/12945 or "dye + varnish", 11/1992, pages 829 ff., Are described.

Examples of suitable rheology-controlling additives are those from the patents WO 94/22968 . EP 0 276 501 A 1, EP 0 249 201 A 1 or WO 97/12945 known; crosslinked polymeric microparticles, as described for example in the EP 0 008 127 A 1 are disclosed; inorganic phyllosilicates such as aluminum-magnesium silicates, sodium magnesium and sodium magnesium fluorine lithium phyllosilicates of the montmorillonite type; Silicas such as aerosils; or synthetic polymers having ionic and / or associative groups such as polyvinyl alcohol, poly (meth) acrylamide, poly (meth) acrylic acid, polyvinylpyrrolidone, styrene-maleic anhydride or ethylene-maleic anhydride copolymers and their derivatives or hydrophobically modified ethoxylated urethanes or polyacrylates.

An example of a suitable matting agent is magnesium stearate.

Examples of suitable precursors for organically modified ceramic materials are hydrolyzable organometallic compounds, in particular of silicon and aluminum.

Further examples of the additives listed above, as well as examples of suitable UV absorbers, radical scavengers, leveling agents, flame retardants, siccatives, drying agents, skin preventatives, corrosion inhibitors and waxes (B) are described in the textbook "Lackadditive" by Johan Bieleman, Wiley-VCH, Weinheim, New York, 1998, described in detail.

Further examples of additives are dyes, colored pigments, white pigments, fluorescent pigments and phosphorescent pigments (phosphors) and the materials described below.

Carbohydrates:

Glyceraldehyde, erythrose, threose, ribose, arabinose, xylose, lyxose, fructose, allose, altrose, glucose, mannose, idose, galactose talose, rhamnose, amino sugars such as neuraminic acid, muramic acid, glucosamine, mannosamine, aldonic acids, ketoaldonic acids, aldaric acids, pyranoses, sucrose , Lactose, raffinose, panose and homopolysaccharides and heteropolysaccharides and proteoglycans, wherein the polysaccharide portion outweighs the protein portion, such as starch, dextran, cyclodextrin, arabinogalactan, celluloses, modified celluloses, lignocelluloses, chitin, chitosan, carageen and glycosaminoglycans.

Monoalcohols:

Methanol, ethanol, propanol, isopropanol, butanol, isobutanol, tert-butanol, amyl alcohol isoamyl alcohol, cyclopentanol, hexanol, cyclohexanol, heptanol, octanol, nonanol, decanol, undecanol, dodecanol and their stereoisomers.

polyols:

Glycerol, trimethylolpropane, pentaerythritol, alditols, cyclitols, dimers and oligomers of glycerin, trimethylolpropane, pentaerythritol, alditols and cyclitols; preferably tetritols, pentitols, hexitols, heptitols and octitols; preferably arabinitol, ribitol, xylitol, erythritol, threitol, galactitol, mannitol, glucitol, allitol, altritol, iditol, maltitol, isomaltitol, lactitol, tri-, tetra-, penta-, hexa-, hepta-, octa-, nona-, Deca-, undeca- and dodecaglycerol, - trimethylolpropane, -erythritol, -threitol and -pentaerythritol, 1,2,3,4-tetrahydroxycyclohexanes, 1,2,3,4,5-pentahydroxycyclohexanes, myo-, scyllo-, muco- , chiro-, neo-, allo-, epi- and cis-inositol.

polyhydroxycarboxylic:

Glycerol, citric, tartaric, threonine, erythron, xylon, ascorbic, glucone, galacturon, iduron, mannuron, glucuron, guluron, glycuron, glucar, uluson, diketogulone, and lactobionic.

Polyhydroxyphenols and -benzenecarboxylic acids:

Pyrocatechol, resorcinol, hydroquinone, pyrogallol, 1,2,4-trishydroxybenzene, phloroglucinol, 2,3-, 2,4-, 2,5-, 2,6-, 3,4- and 3,5-dihydroxybenzoic and 2,4,6-, 2,4,5-, 2,3,4- and 3,4,5-trihydroxybenzoic acid (bile acid).

Amine:

Ammonia, ammonium, mono-, di- and trialkyl-, -aryl-, cycloalkyl-, -alkylaryl-, -alkylcycloakyl-, -cycloalkylaryl- and -alkylcycloalkylarylamines such as methylamine, ethylamine, propylamine, isopropylamine, butylamine, isobutylamine, tert. Butylamine, benzylamine, cyclohexylamine, dodecylamine, cocoamine, tallowamine, adamantylamine, aniline, ethylenediamine, propylenediamine, butylenediamine, piperidine, piperazine, pyrazolidine, pyrazine, quinuclidine and morpholine.

thiols:

Mercaptopropionic acid, dimercaptosuccinic acid (DMSA), dithiothreitol (DTT) and octadecanethiol.

Click Chemistry:

Compounds for click reactions such as the copper-catalyzed cycloaddition of azides and alkynes, Diels-Alder reactions, reactions of e.g. Folic acid with alkyne groups and dipolar cycloadditions with e.g. Poly (tert-butyl acrylate).

fatty acids:

Lauric, myristic, oleic, palmitic, linoleic, stearic, arachinic and behenic acid.

Polymers and oligomers with functional groups:

Poly (trimethyl ammonium ethyl acrylate), polyacrylamide, poly (D, L-lactide-co-ethylene glycol), Pluronic®, Tetronic®, polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), poly (alkyl cyanoacrylate), poly (lactic acid), poly (epsilon-) caprolactone), polyethylene glycol (PEG), poly (oxyethylene-co-propene) bisphosphonate, poly (acrylic acid), poly (methacrylic acid), hyaluronic acid, alginic acid, pectic acid, poly (ethyleneimine), poly (vinylpyridine), polyisobutene, poly (styrenesulfonic acid) , Poly (glycidyl methacrylate), poly (methacryloyloxyethyltrimethylammonium chloride) (MATAC), poly (L-lysine) and poly (3- (trimethoxysilyl) propylmethacrylate-r-PEG-methylethermethacrylate), proteins such as treptavidin, trypsin, albumin, immunoglobulin, oligo-, and Polynucleotides such as DNA and RNA, peptides such as Arginylglycylasparginsäure (RGD), AGKGTPSLETTP peptide (A54), HSYHSHSLLRMF peptide (C10) and glutathione, enzymes such as glucose oxidase, dendrimers such as polypropyleneimine Tetrahexacontaamin dendrimer generation 5 (PPI G5), poly (amidoamine ) (PAMAM) and guanidine dendrimers, Phosphonic acid and dithiopyridine functionalized polystyrenes, functionalized polyethylene glycols (PEG: degree of polymerization 4-10, especially 5) such as PEG (5) nitroDOPA, nitrodopamine, mimosine, hydroxydopamine, hydroxypyridine, hydroxypyrone and carboxyl.

complexing agents:

Complexones such as nitrilotriacetic acid (NTA) and ethylenediaminetetraacetic acid (EDTA), phosphonic acids such as [(2-aminoethyl) hydroxymethylene] diphosphonic acid and [(5-aminopentyl) hyroxymethylene] diphosphonic acid and crown ethers.

Metal Complexes:

Usual and known coordination, sandwich and chelate complexes of the abovementioned metals and their cations with organic and inorganic anions, in particular fluoride, chloride, bromide, iodide, ammonia, amines, phosphines, thiols, sulfides, cyanide, cyanate, isocyanate, thiocyanate, Isothiocyanate, boranes, carbon monoxide, aromatics or heteroaromatics.

The above-listed functional groups and materials for the sheaths of the POM microparticles and POM nanoparticles are given by way of example only and not exhaustively. The enumeration is therefore intended to illustrate the variety of possibilities, and the expert can readily specify other possibilities due to his general expertise.

Another preferred ingredient is water. The water content can vary widely and also depends essentially on the intended use. For example, the water may be present as water of crystallization and / or be adsorbed on the surface of the POM microparticles and POM nanoparticles to be used according to the invention.

The POM microparticles and POM nanoparticles to be used according to the invention can be added to or mixed with other diamagnetic, non-magnetizable micro- and / or nanoparticles, but preferably nanoparticles. The POM microparticles and POM nanoparticles and the diamagnetic micro- and / or nanoparticles may be bound together by covalent and / or ionic bonds, hydrogen bonds, electrostatic attraction, and / or van der Waals forces.

• - Oxide aus der Gruppe, bestehend aus Scandiumoxid, Yttriumoxid, Titandioxid, Zirconiumdioxid, Yttrium-stabilisiertes Zirconiumdioxid, Hafniumdioxid, Vanadiumoxid, Nioboxid, Tantaloxid, Manganoxid, Eisenoxid, Chromoxid, Molybdänoxid, Wolframoxid, Zinkoxid, Oxide der Lanthanide, bevorzugt Lanthanoxid und Ceroxid, insbesondere Ceroxid, Oxide der Actinide, Magnesiumoxid, Calciumoxid, Strontiumoxid, Bariumoxid, Aluminiumoxid, zinkdotiertes Aluminiumoxid, Galliumoxid, Indiumoxid, Siliziumdioxid, Germaniumoxid, Zinnoxid, Antimonoxid, Bismutoxid, Zeolithe, Spinelle, Mischoxide aus mindestens zwei der genannten Oxide wie Antimon-Zinn-Oxid, Indium-Zinn-Oxid, Bariumtitanat, Bleititanat oder Bleizirkonattitanat;
• - Phosphate wie Hydroxylapatit oder Calciumphosphat;
• - Sulfide, Selenide und Telluride aus der Gruppe, bestehend aus Arsen-, Antimon-, Wismut-, Cadmium-, Zink-, Eisen-, Silber-, Blei- und Kupfersulfid, Cadmiumselenid, Zinnselenid, Zinkselenid, Cadmiumtellurid und Bleitellurid;
• - Selen und Selendioxid (vgl. M. Shakibaie et al, »Anti-biofilm activity of biogenic selenium nanoparticles and selenium dioxide against clinical isolates of Staphylococcus aureus, Pseudomonas aeruguinosa, and Proteus mirabilis«, Journal of Trace Elements in Medicine and Biology, Bd. 29, Januar 2015, Seiten 235 bis 241 );
• - Nitride wie Bornitrid, Siliziumnitrid, Aluminiumnitrid, Galliumnitrid und Titannitrid;
• - Phosphide, Arsenide und Antimonide aus der Gruppe, bestehend aus Aluminiumphosphid Galliumphosphid, Indiumphosphid, Aluminiumarsenid, Galliumarsenid, Indiumarsenid, Aluminiumantimonid, Galliumantimonid, Indiumantimonid;
• - Zintl-Phasen wie Na₄Sn₉, Na₄Pb₉, Na₂Pb₁₀, Na₃[Cu@Sn₉], Na₇[Ge₉CuGe₉] oder Na₁₂[Sn₂@Cu₁₂Sn²⁰];
• - Kohlenstoff wie Fullerene, Graphen, Graphit, Diamant und funktionalisierte und nicht funktionalisierte Kohlenstoff-Nanoröhrchen;
• - Nanocellulose-Partikel aus der Gruppe, bestehend aus Cellulosenanofasern (CNF), mikrofibrilläre Cellulose (MFC), nanokristalline Cellulose (CNC) und bakterielle Nanocellulose (BNC), ausgewählt. Die Mikrocellulose-Partikel sind vorzugsweise die mikrokristallinen Cellulosen (MCC), wie sie beispielsweise von der Firma DFE Pharma unter der Marke Pharmacel® angeboten werden;
• - metallorganische Gerüstverbindungen (MOFs);
• - Carbide wie Borcarbid, Siliziumcarbid, Wolframcarbid, Titancarbid oder Cadmiumcarbid;
• - Boride wie Zirkonborid; sowie
• - Silicide wie Molybdänsilicid.

Examples of suitable materials from which the diamagnetic micro- and / or nanoparticles can be constructed are in particular

• Oxides from the group consisting of scandium oxide, yttrium oxide, titanium dioxide, zirconium dioxide, yttrium-stabilized zirconium dioxide, hafnium dioxide, vanadium oxide, niobium oxide, tantalum oxide, manganese oxide, iron oxide, chromium oxide, molybdenum oxide, tungsten oxide, zinc oxide, oxides of lanthanides, preferably lanthanum oxide and cerium oxide, in particular, ceria, oxides of actinides, magnesium oxide, calcium oxide, strontium oxide, barium oxide, aluminum oxide, zinc-doped aluminum oxide, gallium oxide, indium oxide, silicon dioxide, germanium oxide, tin oxide, antimony oxide, bismuth oxide, zeolites, spinels, mixed oxides of at least two of the abovementioned oxides, such as antimony-tin oxide. Oxide, indium-tin oxide, barium titanate, lead titanate or lead zirconate titanate;
• - phosphates such as hydroxyapatite or calcium phosphate;
• - sulfides, selenides and tellurides from the group consisting of arsenic, antimony, bismuth, cadmium, zinc, iron, silver, lead and copper sulfide, cadmium selenide, tin selenide, zinc selenide, cadmium telluride and lead telluride;
• - selenium and selenium dioxide (cf. M. Shakibaie et al, "Anti-biofilm activity of biogenic selenium nanoparticles and selenium dioxide against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis," Journal of Trace Elements in Medicine and Biology, Vol. 29, January 2015, pp 235 to 241 );
• Nitrides such as boron nitride, silicon nitride, aluminum nitride, gallium nitride and titanium nitride;
• Phosphides, arsenides and antimonides selected from the group consisting of aluminum phosphide gallium phosphide, indium phosphide, aluminum arsenide, gallium arsenide, indium arsenide, aluminum antimonide, gallium antimonide, indium antimonide;
• Zintl phases such as Na ₄ Sn ₉ , Na ₄ Pb ₉ , Na ₂ Pb ₁₀ , Na ₃ [Cu @ Sn ₉ ], Na ₇ [Ge ₉ CuGe ₉ ] or Na ₁₂ [Sn ₂ @ Cu ₁₂ Sn ²⁰ ];
• - Carbon like fullerenes, graphene, graphite, diamond and functionalized and non-functionalized carbon nanotubes;
• Nanocellulose particles selected from the group consisting of cellulose nanofibers (CNF), microfibrillar cellulose (MFC), nanocrystalline cellulose (CNC) and bacterial nanocellulose (BNC). The microcellulose particles are preferably the microcrystalline celluloses (MCC), such as those offered by the company DFE Pharma under the brand Pharmacel®;
• - organometallic frameworks (MOFs);
• Carbides, such as boron carbide, silicon carbide, tungsten carbide, titanium carbide or cadmium carbide;
• - borides such as zirconium boride; such as
• Silicides such as molybdenum silicide.

In particular, micro- and / or nanoparticles of physiologically inert materials are used.

The molecular POM, POM microparticles and POM nanoparticles to be used according to the invention can be distributed homogeneously or inhomogeneously in an organic and / or inorganic matrix, in particular an organic and / or inorganic polymeric matrix and / or in an inorganic ceramic matrix.

In an inhomogeneous distribution, the content of molecular POM, POM microparticles, and POM nanoparticles in the matrix is a function of the distance from the surface of the matrix. For example, the concentration of molecular POM, POM microparticles, and POM nanoparticles may increase or decrease continuously or discontinuously with distance from the surface.

A particular advantage of this embodiment is that in the mechanical, physical and / or chemical removal of the material of the object in question a fresh active, POM-containing surface is exposed time and again.

However, the POMs to be used according to the invention can also be present as a separate layer on the surface of the matrix. This embodiment has the advantage of saving material.

In addition, both embodiments can be combined.

The organic polymeric matrix may be composed of conventional and known thermoplastic or thermoset polymers.

Suitable thermoplastic polymers are customary and known linear and / or branched and / or block-like, comb-like and / or random polyaddition resins, polycondensation resins and / or (co) polymers of ethylenically unsaturated monomers.

Examples of suitable (co) polymers are (meth) acrylate (co) polymers and / or polystyrene, polyvinyl esters, polyvinyl ethers, polyvinyl halides, polyvinylamides, polyacrylonitriles, polyethylenes, polypropylenes, polybutylenes, polyisoprenes and / or copolymers thereof.

Examples of suitable polyaddition resins or polycondensation resins are polyesters, alkyds, polylactones, polycarbonates, polyethers, proteins, epoxy resin-amine adducts, polyurethanes, alkyd resins polysiloxanes, phenol-formaldehyde resins, urea-formaldehyde resins, melamine-formaldehyde resins, cellulose, polysulfides , Polyacetals, polyethylene oxides, polycaprolactams, polylactones, polylactides, polyimides, and / or polyureas.

As is known, the thermosets are prepared from polyfunctional, low molecular weight and / or oligomeric compounds by (co) polymerization initiated thermally and / or with actinic radiation. Suitable functional low molecular weight and / or oligomeric compounds are the reactive diluents, catalysts and initiators listed above.

Again, the list of thermoplastics and thermosets listed above is not exhaustive, but in particular to illustrate the variety of possibilities. Other suitable materials for the polymeric matrix can be readily selected by those skilled in the art, based on their general knowledge.

Once the polymeric matrix is built up from thermoplastics, the molecular POM, POM microparticles, and POM nanoparticles are incorporated into the thermoplastics by conventional and well known methods of making polymer blends.

If the polymeric matrix is composed of thermosets, the POM microparticles and POM nanoparticles are incorporated into the starting materials of the thermosets by means of customary and known mixing methods, after which the resulting mixtures are polymerized and crosslinked thereby.

For mixing the materials, it is possible to use the customary and known mixing units, such as high-speed stirrers, Ultraturrax, inline dissolvers, homogenizing nozzles, static mixers, microfluidizers, extruders or kneaders.

The inorganic ceramic matrix may be composed of conventional and known glasses and / or ceramic materials.

The ceramic may be constructed of an oxide ceramic and / or non-oxide ceramic. Examples of suitable ceramics are aluminum oxide, boron carbide, boron nitride, boron nitride carbide, calcium silicate, hafnium carbide, silicon oxide, silicon carbide, silicon nitride, silicon oxynitride, silicon oxide carbide, silicon nitride carbide, silicon oxynitride carbide, glass ceramic, tantalum carbide, zinc carbide or zirconia ceramics composed of alumina, boron carbide, boron nitride, boron nitride carbide, calcium silicate, hafnium carbide, silica, silicon carbide, silicon nitride, silicon oxynitride, silicon oxide carbide, silicon nitride carbide, silicon oxynitride carbide, silicon aluminum oxynitride, glass ceramic, tantalum carbide, zinc carbide and / or zirconia.

In contrast to all other materials that ceramic products, especially oxide ceramics, first formed from the raw materials and then (ie after molding) in a high-temperature process or sintering process with the goal of material conversion to produce cohesive connections between the raw material grains in the ceramic material be transferred. In contrast to the other materials, the raw materials have two fundamental tasks: on the one hand they must guarantee the chemical composition of the desired ceramic materials and on the other hand they must first allow their shaping. The ceramic blank thus has a significantly lower mechanical strength than, for example, a metallic blank. Therefore bears the name Grünling, which has nothing to do with the color.

1. (i) Erzeugung der Rohstoffe,
2. (ii) Herstellung der keramischen Masse,
3. (iii) Formgebung,
4. (iv) Entfernung der Hilfsmittel wie Wasser und/oder organische Additive für die Formgebung,
5. (v) Gegebenenfalls mechanische Bearbeitung der Rohlinge oder Glasieren,
6. (vi) keramischer Brand sowie
7. (vii) Unterschiedliche Verfahren der Nachbehandlung und Veredelung einschließlich Glasieren oder Dekorieren und nochmaliger Brand.

The manufacture of the ceramic products, irrespective of the composition, always comprises the following steps:

1. (i) production of raw materials,
2. (ii) preparation of the ceramic mass,
3. (iii) shaping,
4. (iv) removal of auxiliaries such as water and / or organic additives for shaping,
5. (v) Optionally mechanical processing of the blanks or glazing,
6. (vi) ceramic fire as well
7. (vii) Different methods of post-treatment and refinement, including glazing or decorating and re-firing.

Thieme Römpp Online 2014, Version 3.45, »Ceramics, Table 1: Forming process for clay-ceramic compounds with production examples« provides an overview of the production of oxide ceramics. Examples of suitable oxide ceramics go from the German patent applications DE 196 28 820 A1 , ScienceDaily®, Novel Ceramic Foam is Safe and Effective Insulation, May 18, 2001, the company publication "Promat High Performance Insulation, Theoretical Foundations of Thermal Insulation," or the article by F. Luthardt and Jörg Adler, "A Ceramic Foaming Technology for High-Temperature Insulation Materials ", Fraunhofer IKTS Annual Report 2012/13, pages 32 and 33.

Non-oxide ceramics do not contain oxygen. The anions are instead carbon, nitrogen, boron and silicon. Exceptions are a few mixed ceramics which, in addition to the said anion, also contain some oxygen, e.g. Siliciumaluminiumoxidnitrid.

But the cations also differ significantly from the oxide ceramics. When silicon and where as cations occur, the homeopolar bond prevails, so that one can not speak chemically exactly of cation and anion. In contrast, if, for example, titanium, zirconium, niobium or tungsten are present in the crystal lattice, then these form layers with metallic bonds in the material which are heteropolar with the homopolar bonded carbon, nitrogen, boron or silicon layers.

Alkali and alkaline earth cations contained in many oxide ceramics and almost all silicate ceramics are not found in non-oxide ceramics unless as an impurity or, in the exception, as a dopant.

Further details on non-oxide ceramics can be found in Thieme Römpp Online 2014 Version 3.45, "Non-oxide Ceramics". Examples of suitable non-oxide ceramics are further from the American patent application US 2014/0206525 A1 and the German patent applications DE 102 07 860 A1 and DE 10 2012 021 906 A1 out.

Glass-ceramics are polycrystalline solids with more than 30% glass phase which are produced by controlled crystallization of glasses. The crystals are usually colorless by heat treatment of a suitable glass and cause a spatial dispersion of the light entering the material.

• - MgOxAl₂O₃xnSiO₂-System (MAS-System),
• - ZnOxAl2O₃xnSiO₂ (ZAS-System),
• - LiOxAl2O₃xnSiO₂ (LAS-System) und
• - KMg₃[(F, OH)₂AlSi₃O₁₀] (Phlogopit).

Examples of suitable glass ceramics are

• MgOxAl ₂ O ₃ xnSiO ₂ system (MAS system),
• ZnOxAl ₂ O ₃ xnSiO ₂ (ZAS system),
• - LiOxAl2O ₃ xnSiO ₂ (LAS system) and
• - KMg ₃ [(F, OH) ₂ AlSi ₃ O ₁₀ ] (phlogopite).

Further details on glass ceramics can be found in Thieme Römpp Online 2014 Version 3.45, »Glass Ceramics«, in the international patent application WO 2010/081561 A1 , "Optically transparent glass and glass ceramic foams, process for their preparation and their use" and in the article by AM Marques and AM Bernardin, "Ceramic Foams made from Plain Glass Cullets", Qualicer 2008, pages 89 to 93.

Most preferably, calcium silicates are used.

The calcium silicates are conventional and known products available on the market and can be prepared by a hydrothermal process from finely ground raw materials lime and sand in a water suspension with low solids content and additives. The mineralogical transformations in the main phases of tobermorite 5CaO · 6SiO ₂ · 5.5 H ₂ O (about 10% water, up to 650 ° C resistant) and xonotlite 6CaO. 6SiO ₂ · H ₂ O (about 3% water, stable up to 850 ° C) is carried out in autoclave. The anhydrous phase wollastonite 3CaO. 3SiO ₂ increases the temperature resistance as an additive. The dewatering reactions determine the degree of shrinkage and thus the application limits of the material.

The matrices described above may be shaped articles such as spheres, thin disks, nets, cuboids, rhombuses, pyramids, icosahedra or dodecahedra. The matrices can also be controlled release materials.

However, the POMs to be used according to the invention can also be applied to fibers. The fibers can be made into fabrics.

• Samenfasern: wie Baumwolle (CO), Kapok (KP), Pappelflaum, Akon, Bambusfaser, Brennnesselfaser, Hanffaser (HA), Jute (JU), Kenaf, Leinen (LI), Hopfen, Ramie (RA), Hanf,
• Hartfasern: wie Ananas, Caroä, Curauá, Henequen, Neuseeländer Flachs, Sisal (SI), Kokos (CC),
• Wollen und feine Tierhaare: wie, Wolle von Schafen (WO), Alpaka, Lama, Vikunja, Guanako, Angora (WA), Kanin, Kamelhaar (WK), Kaschmir (WS), Mohair (WM),
• grobe Tierhaare: wie Rinderhaar, Rosshaar, Ziegenhaar,
• Seiden: wie zum Beispiel Maulbeerseide (SE), Tussahseide (ST), Muschelseide,
• Fasern aus natürlichen Polymeren: wie cellulosische Fasern, wie beispielsweise Viskose (CV), Modal (CMD), Lyocell (CLY), Cupro (CUP), Acetat (CA), Triacetat (CTA),
• Gummifasern: wie zum Beispiel Gummi,
• Pflanzeneiweißfasern: wie zum Beispiel Sojaproteinfaser, Zein und andere Prolamine,
• Eiweißfasern: Fasern auf der Basis von Casein, Albuminen, Kollagen, Glykoproteinen, Globuline, Elastin, Nucloproteinen, Histonen, Keratin, Chromoproteine, Protaminen, Fibrinogen, Phosphoproteinen, Prolaminen, Myosin, Lipoproteinen und Hydrophobin,
• Fasern auf Basis Stärke bzw. Glukose: wie zum Beispiel Alginatfasern (ALG) oder Chitosanfasern,
• Fasern aus synthetischen biologisch abbaubaren Polymeren: wie Polylactidfasern (PLA) und Polyester (vgl. Biologisch abbaubare Polyester - Neue Wege mit Bismutkatalysatoren, DISSERTATION zur Erlangung des Grades eines Doktors der Naturwissenschaften des Fachbereichs Chemie der Universität Hamburg, vorgelegt von Gesa Behnken, aus Hamburg, Hamburg 2008) und
• Technische Fasern und anorganische Fasern: wie Polyesterfasern, Polyamidfasern und sonstige Kunststofffasern, Kevlafasern, Glasfasern, Kohlenstofffasern, Metallfasern wie Stahlfasern und Mineralfasern wie Basaltsfasern.

Examples of suitable fibers are:

• Seed fibers: such as cotton (CO), kapok (KP), poplar fluff, acon, bamboo fiber, nettle fiber, hemp fiber (HA), jute (JU), kenaf, linen (LI), hops, ramie (RA), hemp,
• Hard fibers: such as pineapple, Caroä, Curauá, Henequen, New Zealander flax, sisal (SI), coconut (CC),
• Wool and fine animal hair: like, wool from sheep (WO), alpaca, llama, vicuña, guanaco, angora (WA), rabbit, camel hair (WK), cashmere (WS), mohair (WM),
• coarse animal hair: such as cattle hair, horsehair, goat hair,
• Silk: such as mulberry silk (SE), tussah silk (ST), sea silk,
• Fibers of natural polymers: such as cellulosic fibers such as viscose (CV), modal (CMD), lyocell (CLY), cupro (CUP), acetate (CA), triacetate (CTA),
• Rubber fibers: such as rubber,
• Vegetable protein fibers: such as soy protein fiber, zein and other prolamins,
• Protein fibers: fibers based on casein, albumins, collagen, glycoproteins, globulins, elastin, Nucloproteins, histones, keratin, chromoproteins, protamines, fibrinogen, phosphoproteins, prolamins, myosin, lipoproteins and hydrophobin,
• Starch-based or glucose-based fibers such as alginate fibers (ALG) or chitosan fibers,
• Fibers made of synthetic biodegradable polymers: such as polylactide fibers (PLA) and polyesters (see Biodegradable polyesters - novel ways with bismuth catalysts, DISSERTATION to obtain the degree of Doctor of Science of the Department of Chemistry, University of Hamburg, submitted by Gesa Behnken, from Hamburg, Hamburg 2008) and
• Technical fibers and inorganic fibers: such as polyester fibers, polyamide fibers and other plastic fibers, Kevlar fibers, glass fibers, carbon fibers, metal fibers such as steel fibers and mineral fibers such as basalt fibers.

The molecular POM, POM microparticles and POM nanoparticles can be supported on porous bodies. Suitable porous bodies are zeolite particles, porous POM particles, porous glass, porous plastic materials or porous organometallic network compounds (see "Porous Organometallic Network Compounds as Support Matrices for Functional Nanoparticles", dissertation by Stefan Hermes, Ruhr University Bochum, 2006). Also included are carbon particles selected from the group consisting of biochars, pyrogenic carbon, biochar, charcoal, char lumber residue, lumber ashes, activated carbons, coal, animal charcoal, animal waste, fumed carbon of different pyrolysis degree, functionalized coals, pre-treated coals, washed coals and extracted coals , considering. In particular, biochar and / or pyrogenic carbon is used. These materials are common and known and go, for example, from the German patent application DE 10 2015 010 041 A1 Paragraphs [0055] to [0064].

The molecular POM, POM microparticles and POM nanoparticles can be ultrasonically deposited in and / or on the materials described above.

Furthermore, chemotherapeutic agents, antibiotics, biocidal materials, DNA, RNA, cerium silver, gold and / or platinum compounds and / or the nutrient media and / or auxiliary media for cell biology described in more detail below are suitable.

Examples of biocides are 10,10'-oxybisphenoxoarsine (OBPA), octylisothiazolinone (OIT), dichloroctylisothiazolinone (DCOIT), butylbenzisothiazolinone (BBIT), iodocarb (3-iodo-2-propynylbutylcarbamate), zinc pyrithione (zinc salt of pyridine-2 thiol-1-oxide), trichlosan (polychlorinated phenoxyphenols), silver ions and silver, especially in the form of silver nanoparticles.

• Chemotherapeutika auf der Basis von Platin: Carboplatin und Cisplatin,
• Alkylierende Chemotherapeutika auf der Basis von Senfgas
• Nitrosoharnstoffderivate: Carmustin (BCNU),
• Antimetabolite: Methotrexat,
• Purinanaloge Metabolite,
• Pyrimidinanaloge Antimetabolite: Fluorouracil (5-FU) und Gemcitabin,
• Hormonale antineoplastische Verbindungen: Goselerin, Leuprolid und Temoxifen,
• Natürliche antineoplastische Verbindungen: Taxane, Doclitaxel und Paclitaxel,
• Leukine: Aldesleukin, Interleukin-2, Etoposid (VP-16), Interferon alfa und Tretinoin (ATRA),
• Antibiotische natürliche neoplastische Verbindungen: Bleomycin, Dactinomycin, Daunarubicin, Doxorubicin und Mitomycin,
• Vinca alkaloid natural antineoplasics: Vinblastin und Vincristin,
• Weitere Medikamente: Daunarubicin HCl, Docetaxel, Doxorubicin HCl, Epoetin alpha, Ganciclovir Natrium, Gentamicinsulfat, Interferon alpha, Leuprolidacetat, Meperidin HCl (Phetidin), Methadon HCl, Ranitidin HCl, Vinblastinsulfat, Zidovudin (AZT) und Fluorouracil + Epinephrin + Bovinecollagen und
• Antibiotika Penicilline, Neomycine, Tetracycline, Quinolone und Gentramycine.

As chemotherapeutic agents, antibiotics and biocides are basically all drugs and / or toxins and / or metabolites into consideration, as for example in the textbook "General and special pharmacology and toxicology," seventh edition, 1998, Spektrum Akademischer Verlag Heidelberg, publisher W. Forth, D. Henschler, W. Rummel and K. Strong. In particular, the medicaments are chemotherapeutics and antibiotics such as

• Platinum based chemotherapeutic agents: carboplatin and cisplatin,
• Alkylating chemotherapeutic agents based on mustard gas
• Nitrosourea derivatives: carmustine (BCNU),
• Antimetabolites: methotrexate,
• Purine-analogous metabolites,
• Pyrimidine analog antimetabolites: fluorouracil (5-FU) and gemcitabine,
• Hormonal antineoplastic compounds: goselerin, leuprolide and temoxifen,
• Natural antineoplastic compounds: taxanes, doclitaxel and paclitaxel,
• Leukine: aldesleukin, interleukin 2 , Etoposide (VP 16 ), Interferon alfa and tretinoin (ATRA),
• Antibiotic natural neoplastic compounds: bleomycin, dactinomycin, daunarubicin, doxorubicin and mitomycin,
• Vinca alkaloid natural antineoplasics: vinblastine and vincristine,
• Other medications include daunarubicin HCl, docetaxel, doxorubicin HCl, epoetin alpha, ganciclovir sodium, gentamicin sulfate, interferon alpha, leuprolide acetate, meperidine HCl (phtidine), methadone HCl, ranitidine HCl, vinblastine sulfate, zidovudine (AZT), and fluorouracil + epinephrine + bovine collagen and
• Antibiotics penicillins, neomycins, tetracyclines, quinolones and gentramycins.

The POMs described above can be used according to the invention as pure compounds, POM nanoparticles and POM microparticles. In addition, they may be used in the form of POM preparations containing at least one of the above materials. In particular, the POM preparations, the at least one POM as acid, salt, soft oxometalate (SOMS), coating and / or dissolved in molecular dispersion, buffered, especially buffered with dipotassium hydrogen phosphate and / or potassium dihydrogen phosphate, suspended, lipophilized and / or fluidized with organic groups , in and / or on compact and / or porous particles, films, fibers and / or sponges and / or in hydrogels, in micelles, in vesicles, in microcapsules, in gels, in powders, in and on coatings, in and on plastics , in and on tablets, in creams, lotions, ointments and / or foams and / or in mixtures and / or in conjunction with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary media for cell biology.

It is particularly advantageous to add buffers to the POM or POM preparations or to the cultures containing POM and / or POM preparations. Examples of suitable buffers are

Phosphate buffered saline solutions:
(PBS of English phosphate buffered saline) is a buffer solution used in biochemistry. The solution contains 137 mM sodium chloride, 2.7 mM potassium chloride and 12 mM total phosphate (in the form of HPO ₄ ^2- and H ₂ PO ₄ ^- ). The pH of the adjusted buffer solution is 7.4. The property as a buffer solution allows working at this constant pH. Due to the different salts, the solution has the osmotic pressure of the human organism (isotonic saline solution). In particular, Dulbecco's Phosphate Buffered Saline (PBS) - as a solution and powder - is used.

• Alsever's solution
• Gey's balanced salt solution (GBSS)
• Puck's balanced salt solution
• Ringer's balanced salt solution (RBSS)
• Simm's balanced salt solution (SBSS)
• TRIS-buffered saline (TBS)
• Tyrode's balanced salt solution (TBSS
• Hanks' Salzlösung: Hanks' Balanced Salt Solution (HBSS) - als Lösung und Pulver
• Earle's Salzlösung: Earle's Balanced Salt Solution (EBSS) - als Lösung

Balanced Salt Solutions:

• Alsever's solution
• Geys balanced salt solution (GBSS)
• Puck's balanced salt solution
• Ringer's balanced salt solution (RBSS)
• Simm's balanced salt solution (SBSS)
• TRIS-buffered saline (TBS)
• Tyrode's balanced salt solution (TBSS
• Hanks 'Saline Solution: Hanks' Balanced Salt Solution (HBSS) - as a solution and powder
• Earle's Saline Solution: Earle's Balanced Salt Solution (EBSS) - as a solution

sodium:
For cells that multiply rapidly in culture, media with a high hydrogen carbonate content or the corresponding addition of sodium bicarbonate to bicarbonate-free media are suitable when the cell culture is carried out in a CO ₂ incubator. The required CO ₂ concentration is related to the desired pH and the NaHCO ₃ concentration of the medium.

The POM and / or POM preparations can be prepared by stirring, whisking, sonication, vortex mixing, loading, insufflation, and / or nebulization with the above described materials, but especially with the cell biological nutrient media and / or cell biology media described below , as dispersing, partially dissolving, non-dissolving or completely dissolving coatings, coatings, release materials, sponges, flakes, foil pieces, textile pieces, pieces of fabric, tablets, glass beads and / or as a coating that makes the surface hard and smooth and / or diffusion-closed inorganic-organic hybrid polymers (for example Worlee® protect) and / or as an article coated with such a coating, mixed and / or brought into contact.

The concentrations of the polyoxometalates in the cell biological nutrient media and / or the nutrient systems and / or the auxiliary media are preferably from 0.001 to 99.99% by weight, preferably from 0.01 to 99.9% by weight and in particular from 0.1 to 99 wt .-%, each based on the respective total amount of the nutrient medium, the multi-system or the auxiliary medium.

According to the invention, the cell biological nutrient media and / or the nutrient systems and / or the auxiliary media must be used in amounts such that the POMs are introduced into the cultures at the experimentally determinable limit concentrations, i. at concentrations where eukaryotes, host cells and viruses and microbial populations do not undergo transient and / or permanent changes.

However, when the sterilization method of the invention for the decontamination and sterilization of methods for decontamination and sterilization of medicaments, cell biological nutrition systems, cell biology culture media and laboratories for cell biology, microbiology, pharmacology, toxicology and medicine and of items, clothing, equipment and apparatus for and in these At least one cleaning agent containing at least one POM and / or at least one POM in dosages ≥ the limit concentrations is used in laboratories.

For the preparation of the cell biological nutrient media, the cell biological nutrient systems and the cell biological auxiliary media, the nutrients and additives can be mixed together according to the selected recipe and dissolved in water and, if necessary, with heating with hot, flowing water vapor. Subsequently, the stabilization is carried out, usually by heating in an autoclave. If thermolabile additives are present which would be destroyed by the heat sterilization, the sterilization can also be carried out by sterile filtration with a membrane filter, after which the sterilized additives are added to the cool sterilized nutrient media, nutrient systems and auxiliary media. In this case, the POM and / or the POM preparations can be used directly in both methods since they are not thermally labile.

• • Bio-Science grade
• • Nuklease frei
• • Autoklaviert
• • DEPC behandeltes Wasser.

The water is preferably used in the following quality levels:

• • Bio-Science grade
• • Nuclease free
• • Autoclaved
• • DEPC treated water.

Mycoplasma-infected and mycoplasma-free cultures of eukaryotes, virus cultures and cultures of microorganism populations are subjected to long-term incubation and short-term incubation with media of different POM limit concentrations.

This can be done in the dark and / or under irradiation with electromagnetic radiation, in particular far infrared, near infrared (NIR), visible light and / or UV radiation. Preferably, visible light, in particular daylight, is used. In the determination of the limiting concentration and in the subsequent experiments, it is only possible to work in the dark, alternately in the dark and under irradiation with electromagnetic radiation or only with irradiation.

The experiments can also be carried out at atmospheric pressure or overpressure or alternately atmospheric pressure and overpressure.

Furthermore, the experiments can be carried out on dried or moist air or under inert gas such as carbon dioxide, nitrogen, helium, neon, argon, krypton and / or xenon.

Furthermore, the experiments at 0 ° C to 100 ° C, preferably 15 ° C to 80 ° C and in particular 20 ° C to 50 ° C are performed.

In the use according to the invention, the POM and / or the POM are applied in an experimentally determinable limiting concentration or concentration <Grenzkonzentration after a culture incubation period of 0 to permanently, preferably from 30 minutes to 100 days, preferably from 30 minutes 50 days and in particular 30 minutes to 10 days and a Mycoplasendetektion after a life of the incubated cultures from 0 to permanently, preferably from 30 minutes to 100 days, preferably 30 minutes to 50 days and especially 30 minutes to 10 days, the proliferation of unicellular and / or multicellular eukaryotes, host cells and viruses as well as microorganism populations in the respective cultures. These limiting concentrations are determined in separate test series with mycoplasma-free or mycoplasma-infected cultures of the unicellular and / or multicellular eukaryotes, host cells and viruses to be examined, as well as microorganism populations. The conditions of the incubation and the devices for this purpose are determined by the respective unicellular and / or multicellular eukaryotes to be investigated, the viruses and the host cells as well as the microorganism populations and are known to the person skilled in the art.

For example, for cultures of cell line A549 (cells of explanted adenocarcinoma) infected with Mycoplasma hominis, this limit concentration for tungstophosphoric acid (Wo-Pho), ammonium heptamolybdate (AHMT) and tungstosilicic acid (WCS) is below 10 mM, respectively. For cultures of the cell line 3T3-L1 (Swiss albino cells) infected with Mycoplasma hominis, this limit concentration is below 10 mM for Wo-Pho and below 5 mM for WCS.

In one embodiment, the POM cutoff concentration for an incubation period of the infected cultures of 30 minutes and a mycoplasma detection is <15 mM after a further 5 days. At an incubation time of 4 days and with a mycoplasma detection after another 5 days, it is <1.0 mM.

The following are examples of suitable materials used in the context of the present invention for the use according to the invention, the cultures according to the invention, the kits according to the invention, the proliferation method according to the invention, the sterilization method according to the invention and the articles and fluids according to the invention.

Since mycoplasmas are also inside the cells (intracellular), it makes sense to combine the POM with carriers that transport them through the membrane. This can enhance the beneficial effects of POM. In the following, suitable carriers are listed in tabular form.

• ■ DMSO
• ■ DMF

Chemical carrier

• ■ DMSO
• ■ DMF

• ■ Electrophorese
• ■ Elektroschock
• ■ Scherkräfte

Physical Carrier

• ■ electrophoresis
• ■ electric shock
• ■ Shear forces

• ■ Polymere Konjugate
• ■ Polymeren Nanopartikeln,
• ■ Carrier auf der Basis von Lipiden, insbesondere Carrier, die Lipide und Mizellen enthalten
• ■ Dendrimere
• ■ Kohlenstoff-Nanoröhrchen
• ■ Gold-Nanopartikeln, Gold-Nanohüllen, Gold-Nanokäfige
• ■ Virushüllen, z.B. vom Tabakmosaikvirus

Nanocarrier

• ■ Polymeric conjugates
• ■ polymeric nanoparticles,
• ■ Carrier based on lipids, especially carriers containing lipids and micelles
• ■ dendrimers
• ■ Carbon nanotubes
• ■ Gold nanoparticles, gold nanoshells, gold nanocages
• ■ Virus envelopes, eg from tobacco mosaic virus

Biological carrier

• ■ Major Facilitator-Proteine (mit den Glucosetransportern),
• ■ Solute: Natrium-Symporter (SSS),
• ■ Mitochondriale Carrier,
• ■ Chloridcarrier/-kanäle,
• ■ Organo-Anion-Transporter und
• ■ Oligopeptid-Transporter

Transporters (also: carriers or permeases) are membrane-bound transport proteins:

• Major facilitator proteins (with the glucose transporters),
• ■ Solute: Sodium Symporter (SSS),
• ■ mitochondrial carrier,
• ■ chloride carriers / channels,
• ■ Organo-anion transporters and
• ■ oligopeptide transporters

1. I. Zussammensetzung,
2. II. Nährstoffansprüche,
3. III. Arbeitsaufwand,
4. IV. Untersuchungsziel,
5. V. Replica Plating,
6. VI. Beispiele und
7. VII. Hilfsmedien.

The nutrient media suitable for the use according to the invention can be subdivided according to the following criteria:

1. I. Composition,
2. II. Nutrient claims,
3. III. Labor,
4. IV. Study objective,
5. V. Replica Plating,
6. VI. Examples and
7. VII. Auxiliary media.

Composition in list form:

• o Wasser (siehe oben)
• o Eine für den jeweiligen Organismus verwertbaren Energiequelle (siehe Chemotrophie), beispielsweise organische Verbindungen oder schwefelhaltige Verbindungen,
• o Von ihm benötigte Nährstoffe (organische oder anorganische Kohlenstoff-, Stickstoff-, Schwefel- und Phosphat-Quellen sowie andere essentielle Nährstoffe).
• ◯ Kohlenhydrate („Zucker“),
• ◯ Proteinhydrolysate (Peptone) und
• ◯ Fettsäuren.
• ◯ Salze, wie z. B. Ammonium, Kalium, Natrium, Phosphat, Sulfat sowie Spurenelemente. [2]
• ◯ Farbstoffe bzw. deren Vorstufen (Mikroskopiefarbstoffe, chromogene Substrate)
• ◯ Geliermittel (Verfestigungsmittel), wie Agar-Agar oder (seltener) Gelatine,
• ◯ Hemmstoffe (zum Beispiel Antibiotika)
• ◯ Selektive Agentien, um das Wachstum unerwünschter Mikroorganismen zu verhindern (z. B. Chloramphenicol für Hefen/Schimmel-Nährböden)
• ◯ Indikatoren, um Änderungen anzuzeigen, wie z. B. beim pH-Wert, aber auch um gewisse Stoffwechselprodukte oder Stoffwechselaktivitäten anzuzeigen
• ◯ Puffersubstanzen, um den pH-Wert zu stabilisieren
• ◯ Wachstumsfaktoren (Suppline genannt), wie Hormone, Vitamine und dergleichen.
• ◯ Feste Nährmedien enthalten mindestens 1 % Agar-Agar, je nach Rezeptur variiert die Konzentration zwischen 10 und 20 g Agar/I Nährmedium (Gramm pro Liter).
• ◯ Für einen sogenannten „Weichagar“ sind 1-4 g/l üblich.
• ◯ Wässrige Lösungen komplexer, organischer Substrate von Bouillon
• ◯ Vorsterilisiert, ready to use, und nicht steril
• ◯ Mit und ohne Phenolrot oder anderen Indikatoren
• ◯ Mit und ohne Puffer
• ◯ Als Pulver, Fluessigkeit, lypophilisiert, gefriergetrocknet oder Gel
• ◯ Vorsterilisiert, ready to use, und nicht steril
• ◯ DNAse-, Protease- und RNAse-frei oder DNAse-, Protease- und RNAse-haltig

In general, the nutrient media may have the following components.

• o water (see above)
• o an energy source which can be used for the respective organism (see Chemotrophy), for example organic compounds or sulfur-containing compounds,
• o Nutrients it needs (organic or inorganic carbon, nitrogen, sulfur and phosphate sources, as well as other essential nutrients).
• ◯ carbohydrates ("sugar"),
• Protein hydrolysates (peptones) and
• ◯ fatty acids.
• ◯ salts, such as. As ammonium, potassium, sodium, phosphate, sulfate and trace elements. [2]
• ◯ dyes or their precursors (microscopy dyes, chromogenic substrates)
• ◯ gelling agent (solidifying agent), such as agar-agar or (more rarely) gelatine,
• ◯ inhibitors (for example antibiotics)
• ◯ Selective agents to prevent the growth of unwanted microorganisms (eg chloramphenicol for yeasts / mold media)
• ◯ Indicators to indicate changes, such as: As the pH value, but also to indicate certain metabolic products or metabolic activities
• ◯ buffer substances to stabilize the pH
• ◯ Growth factors (called suppline), such as hormones, vitamins and the like.
• ◯ Solid nutrient media contain at least 1% agar-agar, depending on the recipe, the concentration varies between 10 and 20 g agar / I medium (grams per liter).
• ◯ For a so-called "soft agar" 1-4 g / l are common.
• ◯ Aqueous solutions of complex, organic substrates of bouillon
• ◯ Pre-sterilized, ready to use, and not sterile
• ◯ With and without phenol red or other indicators
• ◯ With and without buffer
• ◯ Powder, liquid, lyophilized, freeze-dried or gel
• ◯ Pre-sterilized, ready to use, and not sterile
• ◯ DNAse, protease and RNAse-free or DNAse, protease and RNAse-containing

• • USP (United States Pharmacopeia)
• • Steril
• • Pyrogenfrei
• • Hypotonisch
• • Niedrige oder keine Endotoxine
• • Isotonisch
• • Verschiedene pH-Werte von sauer bis basisch, bevorzugt 7,2
• • Bakteriengefiltert
• • Destilliert
• • Demineralisiert
• • Dekontaminiert
• • Steril filtirert und dampfsterilisiert

'Specific characteristics:

• • USP (United States Pharmacopeia)
• • Sterile
• • Pyrogen free
• • Hypotonic
• • Low or no endotoxins
• • Isotonic
• • Different pH values from acidic to basic, preferably 7.2
• • Bacteria filtered
• • Distilled
• • Demineralized
• • Decontaminated
• • Sterile filtertert and steam-sterilized

• ◯ L-Glutamin
• ◯ Natriumpyruvat
• ◯ Nichtessentielle Aminosäuren
• ◯ Natriumbicarbonat
• ◯ Phenolrot
• ◯ Serum/ fetales Kälberserum, menschliches
• ◯ anorganische Salze
• ◯ Aminosäuren
• ◯ Kohlenhydrate
• ◯ Vitamine
• ◯ Peptide und Proteine Lipide und Fettsäuren Spurenelemente
• ◯ mit und ohne L-Gluatimin
• ◯ mit high, low und keiner Glykose
• ◯ reduziertes Serum medium
• ◯ mit und ohne HEPES
• ◯ Mit Glutamin, mit Pyvuvat
• ◯ Mit glutamin ohne Pyruvat
• ◯ Mit stabilem Glutamat, mit und ohne Pyruvat mit und ohne HEPES
• ◯ Mit und ohne Nukleoside
• ◯ Mit und ohne Galactose
• ◯ Mit und ohne Ca/Mg
• ◯ Makronährstoffe
• ◯ Magnesiumsulfat (MgSO₄ · 7H₂O)
• ◯ Kaliumnitrat (KNO₃)
• ◯ Kaliumchlorid (KCI)
• ◯ Kaliumdihydrogenphosphat (KH₂PO₄)
• ◯ Calciumnitrat (Ca(NO₃)₂ . 4H₂O)
• ◯ Mikronährstoffe
• ◯ NaFeEDTA (Ethylendiamin-tetraessigsäure Eisen(III) Natriumsalz)
• ◯ Kaliumiodid (KI)
• ◯ Manganchlorid (MnCl₂ . 4H₂O)
• ◯ Zinksulfat (ZnSO₄ . 7H₂O)
• ◯ Borsäure (H₃Bo₃)
• ◯ Kupfersulfat (CuSO₄ · 5H₂O)
• ◯ Natriummolybdat (Na₂MoO₄ . 2H₂O)
• ◯ Organische Additive
• ◯ Glycin
• ◯ Thiamin
• ◯ Pyridoxin
• ◯ Nicotinsäure
• ◯ Inosit
• ◯ Agar
• ◯ Saccharose
• ◯ Phenolrot oder anderen Indikatoren
• ◯ Puffer
• ◯ L-Gluatimin
• ◯ mit high, low und keiner Glykose
• ◯ reduziertes Serum medium
• ◯ HEPES (2-(4-(2-Hydroxyethyl)-1-piperazinyl)-ethansulfonsäure
• ◯ Glutamin
• ◯ stabilem Glutamat
• ◯ Pyruvat
• ◯ Nukleoside
• ◯ Galactose
• ◯ Ca/Mg
• ◯ Bicarbonat-
• ◯ Glucose
• ◯ MEM-NEAA (Minimum Essential Medium -Nichtessentielle Aminosäuren)
• ◯ Hank's BSS (Balanced Salt Solution)
• ◯ DPBS (Dulbeccos Phosphatgepufferte Salzlösung)
• ◯ Albumin
• ◯ Albumin, endotoxin geprueft
• ◯ Albumin Franktion V, endotoxinarm
• ◯ Dimethylsulfoxid
• ◯ Flavin-adenine-dinucleotid dinatriumsalz
• ◯ Pufferan
• ◯ Lactalbumin
• ◯ Phosphat gepufferte Salze
• ◯ Proteinhydrolysate (Peptone) und
• ◯ Fettsäuren.
• ◯ Salze, wie z. B. Ammonium, Kalium, Natrium, Phosphat, Sulfat sowie Spurenelemente
• ◯ Farbstoffe bzw. deren Vorstufen (Mikroskopiefarbstoffe, chromogene Substrate)
• ◯ Geliermittel (Verfestigungsmittel), wie Agar-Agar oder (seltener) Gelatine,
• ◯ Hemmstoffe (zum Beispiel Antibiotika)
• ◯ Selektive Agentien, um das Wachstum unerwünschter Mikroorganismen zu verhindern (z. B. Chloramphenicol für Hefen/Schimmel-Nährböden)
• ◯ Indikatoren, um Änderungen anzuzeigen, wie z. B. beim pH-Wert, aber auch um gewisse Stoffwechselprodukte oder Stoffwechselaktivitäten anzuzeigen
• ◯ Puffersubstanzen, um den pH-Wert zu stabilisieren
• ◯ Wachstumsfaktoren (Suppline genannt), wie Hormone, Vitamine und dergleichen.
• ◯ Feste Nährmedien enthalten mindestens 1 % Agar-Agar, je nach Rezeptur variiert die Konzentration zwischen 10 und 20 g Agar/I Nährmedium (Gramm pro Liter).
• ◯ Für einen sogenannten „Weichagar“ sind 1-4 g/l üblich.
• ◯ wässrigen Lösungen komplexer, organischer Substrate von Bouillon
• ◯ L-Glutamin Natriumpyruvat
• ◯ Nichtessentielle Aminosäuren
• ◯ Natriumbicarbonat
• ◯ Phenolrot
• ◯ Serum/ fetales Kälberserum, menschliches
• ◯ anorganische Salze
• ◯ Aminosäuren
• ◯ Kohlenhydrate
• ◯ Vitamine
• ◯ Peptide und Proteine Lipide und Fettsäuren Spurenelemente

Other ingredients:

• ◯ L-glutamine
• ◯ Sodium pyruvate
• ◯ Nonessential amino acids
• ◯ Sodium bicarbonate
• ◯ phenol red
• ◯ serum / fetal calf serum, human
• ◯ inorganic salts
• ◯ amino acids
• ◯ carbohydrates
• ◯ vitamins
• ◯ peptides and proteins lipids and fatty acids trace elements
• ◯ with and without L-gluatimine
• ◯ with high, low and no glucose
• ◯ reduced serum medium
• ◯ with and without HEPES
• ◯ with glutamine, with pyvuvate
• ◯ with glutamine without pyruvate
• ◯ With stable glutamate, with and without pyruvate with and without HEPES
• ◯ With and without nucleosides
• ◯ With and without galactose
• ◯ with and without Ca / Mg
• ◯ macronutrients
• ◯ magnesium sulfate (MgSO ₄ · 7H ₂ O)
• ◯ potassium nitrate (KNO ₃ )
• ◯ potassium chloride (KCI)
• ◯ potassium dihydrogen phosphate (KH ₂ PO ₄ )
• ◯ calcium nitrate (Ca (NO ₃ ) ₂ .4H ₂ O)
• ◯ micronutrients
• ◯ NaFeEDTA (ethylenediamine tetraacetic acid ferric sodium salt)
• ◯ potassium iodide (KI)
• ◯ manganese chloride (MnCl _2, 4H ₂ O)
• ◯ zinc sulfate (ZnSO. ₄ 7H ₂ O)
• ◯ boric acid (H ₃ Bo ₃ )
• ◯ copper sulphate (CuSO ₄ · 5H ₂ O)
• ◯ Sodium molybdate (Na ₂ MoO ₄ .2H ₂ O)
• ◯ Organic additives
• ◯ glycine
• ◯ thiamine
• ◯ pyridoxine
• ◯ nicotinic acid
• ◯ Inositol
• ◯ agar
• ◯ sucrose
• ◯ Phenol red or other indicators
• ◯ buffer
• ◯ L-gluatimine
• ◯ with high, low and no glucose
• ◯ reduced serum medium
• ◯ HEPES (2- (4- (2-hydroxyethyl) -1-piperazinyl) ethanesulfonic acid
• ◯ glutamine
• ◯ stable glutamate
• ◯ pyruvate
• ◯ nucleosides
• ◯ galactose
• ◯ Ca / Mg
• ◯ bicarbonate
• ◯ glucose
• ◯ MEM-NEAA (Minimum Essential Medium -Non-Essential Amino Acids)
• Hank's BSS (Balanced Salt Solution)
• ◯ DPBS (Dulbecco's Phosphate Buffered Saline)
• ◯ albumin
• ◯ albumin, endotoxin tested
• ◯ Albumin fraction V, endotoxin-poor
• ◯ dimethyl sulfoxide
• ◯ Flavin adenine dinucleotide disodium salt
• ◯ buffer
• ◯ lactalbumin
• ◯ phosphate buffered salts
• Protein hydrolysates (peptones) and
• ◯ fatty acids.
• ◯ salts, such as. As ammonium, potassium, sodium, phosphate, sulfate and trace elements
• ◯ dyes or their precursors (microscopy dyes, chromogenic substrates)
• ◯ gelling agent (solidifying agent), such as agar-agar or (more rarely) gelatine,
• ◯ inhibitors (for example antibiotics)
• ◯ Selective agents to prevent the growth of unwanted microorganisms (eg chloramphenicol for yeasts / mold media)
• ◯ Indicators to indicate changes, such as: As the pH value, but also to indicate certain metabolic products or metabolic activities
• ◯ buffer substances to stabilize the pH
• ◯ Growth factors (called suppline), such as hormones, vitamins and the like.
• ◯ Solid nutrient media contain at least 1% agar-agar, depending on the recipe, the concentration varies between 10 and 20 g agar / I medium (grams per liter).
• ◯ For a so-called "soft agar" 1-4 g / l are common.
• ◯ aqueous solutions of complex, organic substrates of bouillon
• ◯ L-glutamine sodium pyruvate
• ◯ Nonessential amino acids
• ◯ Sodium bicarbonate
• ◯ phenol red
• ◯ serum / fetal calf serum, human
• ◯ inorganic salts
• ◯ amino acids
• ◯ carbohydrates
• ◯ vitamins
• ◯ peptides and proteins lipids and fatty acids trace elements

• ◯ ACES (N-(1-Acetamido)-2-aminoethansulfonsäure),
• ◯ Acetat (Natriumacetat),
• ◯ ADA (N-(2-Acetamido)-iminodiessigsäure),
• ◯ AMP (2-Amino-2-methyl-1-propanol),
• ◯ AMPD (2-Amino-2-methyl-1,3-propandiol),
• ◯ AMPSO (N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropansulfonsäure),
• ◯ BES (N,N-Bis-(2-hydroxyethyl)-2-aminoethansulfonsäure),
• ◯ Bicarbonat (Natriumhydrogencarbonat),
• ◯ Bicin (N,N-Bis-(2-hydroxyethyl)-glycin),
• ◯ Bis-Tris (Bis-(2-hydroxyethyl)-imino-tris-(hydroxymethyl)-methan),
• ◯ Bis-Tris-Propan (1,3-Bis-[tris-(hydroxymethyl)-methylamino]-propan),
• ◯ CAPS (3-Cyclohexylamino)-1-propansulfonsäure),
• ◯ CAPSO (3-(Cyclohexylamino)-2-hydroxy-1-propansulfonsäure),
• ◯ CHES (2-(N-Cyclo-hexylamin)-ethansulfonsäure),
• ◯ Citrat (tri-Natriumcitrat),
• ◯ DIPSO (N,N-Bis-(2-hydroxyethyl)-3-amino-2-hydroxypropansulfonsäure),
• ◯ HEPES (4-(2-Hydroxyethyl)-piperazin-1-ethan-sulfonsäure),
• ◯ HEPPS (4-(2-Hydroxyethyl)-piperazin-1-propansulfonsäure),
• ◯ HEPPSO (4-(2-Hydroxyethyl)-piperazin-1 (2-hydroxy)-propansulfonsäure),
• ◯ MES (2-Morpholinoethan-sulfonsäure),
• ◯ MOPS (3-Morpholinopropansulfonsäure),
• ◯ MOPSO (3-Morpholino-2-hydroxypropansulfonsäure),
• ◯ PIPES (Piperazin-1,4-bis-(2-ethansulfon-säure)),
• ◯ POPSO (Piperazin-1,4-bis-(2-hydroxy-propansulfonsäure)), TAPS (N-[Tris-(hydroxymethyl)-methyl]-3-aminopropansulfonsäure),
• ◯ TAPSO (N-[Tris-(hydroxymethyl)-methyl]-3-amino-2-hydroxypropan-sulfonsäure),
• ◯ TES (N-[Tris-(hydroxymethyl)-methyl]-2-aminoethansulfonsäure),
• ◯ Tricin (N-[Tris-(hydroxymethyl)-methyl]-glycin),
• ◯ Tris (Tris-(hydroxymethyl)-aminomethan)
• ◯ Tris-HCI
• ◯ TEA, Triethylamin
• ◯ Maleat
• ◯ Phosphatdihydrate
• ◯ Glycin
• ◯ Citrat
• ◯ Glycylycin
• ◯ Malat
• ◯ Succinat
• ◯ Acetat
• ◯ Propionat
• ◯ Cacodylsaeure
• ◯ Imidazol
• ◯ Ammoniumhydroxid

Buffer:

• ◯ ACES (N- (1-acetamido) -2-aminoethanesulfonic acid),
• ◯ acetate (sodium acetate),
• ◯ ADA (N- (2-acetamido) iminodiacetic acid),
• ◯ AMP (2-amino-2-methyl-1-propanol),
• ◯ AMPD (2-amino-2-methyl-1,3-propanediol),
• ◯ AMPSO (N- (1,1-dimethyl-2-hydroxyethyl) -3-amino-2-hydroxypropanesulfonic acid),
• ◯ BES (N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid),
• ◯ bicarbonate (sodium bicarbonate),
• Bicin (N, N-bis (2-hydroxyethyl) glycine),
• ◯ bis-tris (bis (2-hydroxyethyl) -imino-tris- (hydroxymethyl) -methane),
• ◯ bis-tris-propane (1,3-bis- [tris (hydroxymethyl) -methylamino] -propane),
• ◯ CAPS (3-cyclohexylamino) -1-propanesulfonic acid),
• CAPSO (3- (cyclohexylamino) -2-hydroxy-1-propanesulfonic acid),
• CHES (2- (N-cyclo-hexylamine) -ethanesulfonic acid),
• ◯ citrate (tri-sodium citrate),
• DIPSO (N, N-bis (2-hydroxyethyl) -3-amino-2-hydroxypropanesulfonic acid),
• ◯ HEPES (4- (2-hydroxyethyl) piperazine-1-ethane-sulphonic acid),
• ◯ HEPPS (4- (2-hydroxyethyl) piperazine-1-propanesulfonic acid),
• ◯ HEPPSO (4- (2-hydroxyethyl) piperazine-1 (2-hydroxy) -propanesulfonic acid),
• ◯ MES (2-morpholinoethane-sulphonic acid),
• ◯ MOPS (3-morpholinopropanesulfonic acid),
• ◯ MOPSO (3-morpholino-2-hydroxypropanesulfonic acid),
• ◯ PIPES (piperazine-1,4-bis (2-ethanesulfonic acid)),
• ◯ POPSO (piperazine-1,4-bis (2-hydroxypropanesulfonic acid)), TAPS (N- [tris- (hydroxymethyl) -methyl] -3-aminopropanesulfonic acid),
• TAPSO (N- [tris- (hydroxymethyl) -methyl] -3-amino-2-hydroxypropanesulfonic acid),
• TES (N- [tris- (hydroxymethyl) -methyl] -2-aminoethanesulfonic acid),
• ◯ tricine (N- [tris- (hydroxymethyl) -methyl] -glycine),
• Tris (tris- (hydroxymethyl) -aminomethane)
• ◯ Tris-HCI
• ◯ TEA, triethylamine
• ◯ maleate
• ◯ phosphate dihydrate
• ◯ glycine
• ◯ citrate
• ◯ glycylycine
• Malat
• ◯ succinate
• ◯ acetate
• ◯ Propionate
• ◯ Cacodylic acid
• ◯ imidazole
• ◯ ammonium hydroxide

Amino acids:

• • Alanin
• • Glycin
• • Isoleucin
• • Leucin
• • Methionin
• • Prolin
• • Valin

Aliphatic amino acid side chains

• • Alanine
• • Glycine
• • isoleucine
• • leucine
• • methionine
• • proline
• • Valin

• • Histidin
• • Phenylalanin
• • Tryptophan
• • Tyrosin

Aromatic amino acid side chains

• • histidine
• • phenylalanine
• • Tryptophan
• • tyrosine

• • Asparagin
• • Glutamin

Amidated amino acid side chains

• • asparagine
• • glutamine

• • Cystein
• • Methionin
• •

Sulfur-containing amino acid side chains

• • cysteine
• • methionine
• •

• • Serin
• • Threonin
• • Tyrosin

Hydroxylated amino acid side chains

• • serine
• • Threonine
• • tyrosine

• • Lysin
• • Arginin
• • Histidin

Basic amino acid side chains

• • lysine
• • Arginine
• • histidine

• • Asparaginsäure (dissoziiert zu Aspartat)
• • Glutaminsäure (dissoziiert zu Glutamat)

Acid amino acid side chains

• • aspartic acid (dissociated to aspartate)
• Glutamic acid (dissociated to glutamate)

• • Thyroxin,
• • GABA, (γ-Aminobuttersäure)
• • L-Homoserin
• • Ornithin
• • Citrullin
• • Arininosuccinat
• • L-DOPA, (L-3,4-Dihydroxyphenylalanin)
• • 5-Hydroxytrythophan
• • b-Alanin
• • b-Methylamino-Alanin

Nonproteinogenic amino acids:

• • thyroxine,
• • GABA, (γ-aminobutyric acid)
• • L homoserine
• • Ornithine
• • Citrulline
• • Arininosuccinate
• L-DOPA, (L-3,4-dihydroxyphenylalanine)
• • 5-Hydroxytrythophane
• • b-alanine
• • b-methylamino-alanine

• • Pyrrolysin
• • Selenocystein

Non-canonical amino acids

• • Pyrrolysin
• • selenocysteine

• • L-Alanyl-L-Glutamin
• • L-Arginin
• • L-Arginin-Monohydrochlorid
• • L-Aspargin Monohydrat
• • L-Asparaginsaeure
• • L-Cysteine
• • L-Cysteine Hydrochlorid Monohydrat
• • L-Glutamin
• • L-Glutaminsaeure
• • L-Glutathion reduziert
• • Glycin
• • L-Histidin
• • L-Histidin Hydrochlorid Monohydrat
• • L-Isoleucin
• • L-Lysin hydrochlorid
• • L-Lysin Monohydrat
• • L-Methionin
• • L-Orithin Monohydrat
• • L-Phenylalanin
• • L-Prolin
• • Glutamin
• • L-Serin
• • L-Threonin
• • L-Tryptophan
• • L-Tyrosin
• • L-Valin

Specific amino acids

• • L-alanyl-L-glutamine
• • L-arginine
• • L-arginine monohydrochloride
• • L-Aspargin monohydrate
• • L-aspartic acid
• • L-cysteine
• • L-cysteine hydrochloride monohydrate
• • L-glutamine
• • L-glutamic acid
• • L-glutathione reduced
• • Glycine
• • L-histidine
• • L-histidine hydrochloride monohydrate
• • L-isoleucine
• • L-lysine hydrochloride
• • L-lysine monohydrate
• • L-methionine
• • L-orithine monohydrate
• • L-phenylalanine
• • L-proline
• • glutamine
• • L-serine
• • L-threonine
• • L-tryptophan
• • L-Tyrosine
• • L-valine

• • Ampicillin und das Natriumsalz
• • Geneticindisulfat G418
• • Gentamycinsulfat
• • Hygromycin und als B-Loesung
• • Penicillin und als G-Kaliumsalz
• • Penicillin und als G-Natriumsalz
• • Streptomycinsulfat
• • Tetracylin-Hydrochlorid
• • Vancomycin-Hydrochlorid
• • Pen-Strepmischungen

Antibiotics, in general, in particular:

• • ampicillin and the sodium salt
• • Geneticindisulfate G418
• • gentamycin sulfate
• • hygromycin and as a B solution
• • penicillin and as G potassium salt
• • penicillin and as G-sodium salt
• • streptomycin sulfate
• • tetracycline hydrochloride
• Vancomycin hydrochloride
• • Pen-Strapping

• ◯ Calciumchlorid und das Dihydrat
• ◯ Glycose und die D(+) Glycose und das auch wasserfrei
• ◯ Kaliumacetat
• ◯ Kaliumchlorid
• ◯ Magnesiumacetat und das Tetrahydrat
• ◯ Natriumacetat und das Trihydrat
• ◯ Natriumchlorid
• ◯ Natriumcitrat, tri-Natriumcitrat und das Dihydrat
• ◯ Natriumhydrogencarbonat
• ◯ Bicarbonat
• ◯ Glucose

Sugars and salts in general, in particular:

• ◯ calcium chloride and the dihydrate
• ◯ Glycose and the D (+) Glycose and that too anhydrous
• ◯ potassium acetate
• ◯ potassium chloride
• ◯ magnesium acetate and the tetrahydrate
• ◯ Sodium acetate and the trihydrate
• ◯ sodium chloride
• ◯ sodium citrate, tri-sodium citrate and the dihydrate
• ◯ Sodium bicarbonate
• ◯ bicarbonate
• ◯ glucose

• • Ammoniumeisen-III-citratloesung
• • Bacillus cereus Zusatz
• • Campylobacter-Zusatz
• • Campylobacter-Preston Zusatz
• • Clostridium perfringes Zusatz
• • Eigelb-Emulsion
• • Eigelb-Tellurit Emulsion
• • Legionelle GVPC Zusatz
• • Legionelle Wachstumszusatz
• • Listeria-chromo-Lipase C, Selektix, Oxfort
• • Listeria Palcam
• • MRSA Celxitin
• • RPF (Rabbit Plasma Fibrinogen)
• • TTC (2,3,5-Triphenyl-Tetrazoliumchlorid, Tetrazoliumrot)
• • Yersinia
• • Bengalrosa B
• • Brilliantgruen
• • 4-Bromo-4-chloro-3-indoxyl-N-acetyl-b-D-glucosamid
• • Bromkresolgrün , auch als Natrium- und Kaliumsalz
• • Bromkresolpurpur
• • Bromthymolblau, auch die Salze
• • Cholsaeure
• • Dextrin
• • Eisen-II-sulfatHeptahydrat
• • Esulin Sesquihydrat
• • Fuchsin
• • Gelantine
• • Glycerin
• • IPTG (Isopropyl-β-D-thiogalactopyranosid)
• • Kresolrot
• • Kristallviolett
• • Lactose Monohydrat
• • Methylrot
• • 4-Methylumbelliferyl-n-acetyl-b-D-galactosamid
• • 4-Methylumbelliferyl-n-acetyl-b-D-galactosamid
• • 4-Methylumbelliferyl-b-D-glucopyranosid
• • 4-Methylumbelliferyl-phosphat
• • Natriumdisulfit
• • Natriummolybdat-dihydrat
• • Neutralrot
• • 4-Nitrophenyl-N-acetyl-b-D-glucosaminid
• • 4-nitropheyl-b-D-galactopyranosid
• • Phenolrot
• • Saccharose (und alle anderen Zucker)
• • Tetraxoliumblauchlorid
• • 2,3,5-Triphenyltetrazoliumchlorid
• • X-b-Gal
• • X-Glc
• • X-Glu
• • X-glucuro CHAsalz

Especially for bacteria, but not exclusively, the following components:

• • Ammonium iron III citrate solution
• • Bacillus cereus additive
• • Campylobacter supplement
• • Campylobacter-Preston supplement
• • Clostridium perfringes additive
• • egg yolk emulsion
• Egg yolk tellurite emulsion
• • Legionella GVPC additive
• • Legionella growth supplement
• Listeria chromo-lipase C, Selektix, Oxfort
• • Listeria Palcam
• • MRSA Celxitin
• • RPF (Rabbit Plasma Fibrinogen)
• TTC (2,3,5-triphenyl-tetrazolium chloride, tetrazolium red)
• • Yersinia
• • Rose Bengal B
• • Brilliant green
• 4-Bromo-4-chloro-3-indoxyl-N-acetyl-bD-glucosamide
• • Bromocresol green, also as sodium and potassium salt
• • Bromocresol purple
• Bromothymol blue, also the salts
• • Cholic acid
• • Dextrin
• • ferrous sulfate heptahydrate
• • Esulin sesquihydrate
• • fuchsin
• • Gelatine
• • glycerine
• IPTG (isopropyl-β-D-thiogalactopyranoside)
• • Cresol red
• • Crystal violet
• • Lactose monohydrate
• • Methyl red
• 4-methylumbelliferyl-n-acetyl-bD-galactosamide
• 4-methylumbelliferyl-n-acetyl-bD-galactosamide
• 4-methylumbelliferyl-bD-glucopyranoside
• • 4-methylumbelliferyl phosphate
• • sodium disulfite
• • Sodium molybdate dihydrate
• • Neutral red
• 4-Nitrophenyl-N-acetyl-bD-glucosaminide
• 4-nitropheyl-bD-galactopyranoside
• • Phenol red
• • sucrose (and all other sugars)
• • tetraxolium blue chloride
• • 2,3,5-triphenyltetrazolium chloride
• • Xb Gal
• • X-Glc
• • X-Glu
• • X-glucuro CHA salt

• ◯ DPBS mit und ohne EDTA
• ◯ PBS mit und ohne EDTA
• ◯ EDTA
• ◯ Trypsin
• ◯ Trypsin-Inhibitor

Specific cell culture adjuvants:

• ◯ DPBS with and without EDTA
• ◯ PBS with and without EDTA
• ◯ EDTA
• ◯ trypsin
• ◯ trypsin inhibitor

• ◯ Ficoll 400
• ◯ Polysucrose 400
• ◯ Sep 1077

Cell isolation means

• ◯ Ficoll 400
• ◯ Polysucrose 400
• 10 Sep 1077

• ◯ DOTAP (N-[1-(2,3-Dioleoyloxy)]-N,N,N-trimethylammonium propan methylsulfat)
• ◯ DEAE Dextran (Diethylaminoethyldextran)
• ◯ Fect RNAi und der Kit
• ◯ Fect Plus
• ◯ Insectofect

transfection

• ◯ DOTAP (N- [1- (2,3-dioleoyloxy)] - N, N, N-trimethylammonium propane methylsulfate)
• DEAE Dextran (diethylaminoethyldextran)
• ◯ Fect RNAi and the kit
• ◯ Fect Plus
• ◯ Insectofect

• ◯ Neutralrot
• ◯ Loefflers Methylenblauloesung und Methylenblauloesung
• ◯ Tryphanblau, besonders (C.I. 23850)
• ◯ Testloesungen zur Bestimmung der Zellproliferation wie Rotitest Vital

Cell analysis, such as vitality assay and cell counting

• ◯ neutral red
• ◯ Loefflers Methylene Blue Leaching and Methylene Blue Leaching
• ◯ Tryphan blue, especially (CI 23850)
• ◯ Test solutions for determining cell proliferation such as Rotitest Vital

• ◯ Imaging
• ◯ Flow Cytometry

Proliferation assays for:

• ◯ Imaging
• C flow cytometry

• ◯ 5-Brom-2'-desoxyuridin
• ◯ PBST (Phosphat-gepufferte Salzlösung mit Tween)
• ◯ Tween 20, und andere Tween
• ◯ Histofix
• ◯ DAPI (4',6-Diamidin-2-phenylindol)
• ◯ Methanol

High Throughput Screening:

• 5-bromo-2'-deoxyuridine
• ◯ PBST (phosphate buffered saline with Tween)
• ◯ Tween 20, and other tween
• ◯ Histofix
• DAPI (4 ', 6-diamidin-2-phenylindole)
• ◯ methanol

• ◯ Annexin V
• ◯ PBS
• ◯ Blockierung
• ◯ ImmunoBlock
• ◯ Albunim
• ◯ Albumin, IgG-frei

Apoptosis Assay:

• ◯ Annexin V
• ◯ PBS
• ◯ blocking
• ◯ ImmunoBlock
• ◯ Albunim
• ◯ albumin, IgG-free

• ◯ BCIP (5-Brom-4-chlor-3-indolylphosphat) und das Natriumsalz
• ◯ BCIP-Touidinsalz
• ◯ NBT (p-Nitrotetrazoliumblauchlorid)
• ◯ Rose-phos-Toluidinsalz
• ◯ DAB (3,3'-Diaminobenzidin Tetrahydrochlorid)
• ◯ Phosphatase
• ◯ Meerrettisch-Peroxidase

Immune detections:

• ◯ BCIP (5-bromo-4-chloro-3-indolyl phosphate) and the sodium salt
• ◯ BCIP touidine salt
• ◯ NBT (p-nitrotetrazolium blue chloride)
• ◯ Rose-phos-toluidine salt
• ◯ DAB (3,3'-diaminobenzidine tetrahydrochloride)
• ◯ Phosphatase
• ◯ Horse radish peroxidase

• ◯ DPBS/EDTA mit und ohne Ca/Mg
• ◯ PBS/EDTA
• ◯ Trypsin, z.B. BSE (Bovine Spongiform Encephalopathy) und oder TSE (Transmissible Sponigforme Encephalopathie) frei, auch salzfrei
• ◯ Trypsin Inhibitor wie BAEE (Benzoyl-L-arginine ethyl ester), z.B. salzfrei

Cell passage:

• ◯ DPBS / EDTA with and without Ca / Mg
• ◯ PBS / EDTA
• ◯ Trypsin, eg BSE (Bovine Spongiform Encephalopathy) and / or TSE (Transmissible Spongebrake Encephalopathy) free, also salt-free
• ◯ Trypsin inhibitor such as BAEE (benzoyl-L-arginine ethyl ester), eg salt-free

• ◯ Ficoll 400
• ◯ Polysucrose 400
• ◯ Sep 1077

Cell isolation:

• ◯ Ficoll 400
• ◯ Polysucrose 400
• 10 Sep 1077

• ◯ Tween 20, 40 etc.

Tween:

• ◯ Tween 20, 40 etc.

Auxiliary chemicals:

• • Kohlenwasserstoffe ohne funktionelle Gruppe
• • Halogenkohlenwasserstoffe

• Sauerstoff- und Hydroxyverbindungen

• • Alkohole, wie Methanol, Ethanol, Propanol
• • Aldehyde, wie Formaldehyd
• • Ester, wie Essigsaeureester, milchsäure ethylester
• • Ether, wie Diethylether
• • Ketone wie Isobutylmethylketon, Aceton, Butanon
• • Carbonsäuren, wie Essigsauere

• Stickstoffverbindungen

• • Amine, wie Adamantan
• • Amide
• • Diazoniumsalze
• • Nitroverbindungen, beispielsweise TNT
• • Nitrile

• Schwefelverbindungen

• • Alkanthiole
• • Sulfide
• • Disulfide
• • Ester der Schwefelsäure
• • Sulfone
• • Sulfoxide
• • Thionamide
• • Thiolester
• • Thiosäure

• Phosphorverbindungen

• • Ortho-Phosphorsäure
• • Phosphorsäureester
• • Phosphine, beispielsweise Triphenylphosphin

• Metallorganische Verbindungen

• • Ferrocen

• Aliphatische Kohlenwasserstoffe (Aliphaten)

• • Acyclische Kohlenwasserstoffe
  • ■ gesättigt (Alkane)
  • ■ ungesättigt (Alkene und Alkine)
• • Cyclische Kohlenwasserstoffe
  • ■ gesättigt (Alkane)
  • ■ ungesättigt (Alkene und Alkine
• • Aromatische Kohlenwasserstoffe (Aromaten)
  • ■ einfache Aromaten
  • ■ kondensierte Aromaten
• • Heterocyclen
• • Biochemische Verbindungen
  • ■ Alkaloide,
  • ■ Aminosäuren,
  • ■ Kohlenhydrate,
  • ■ Proteine,
  • ■ Steroide,
  • ■ Terpene,
  • ■ Vitamine

• Anorganische Stoffe

• • Wasserstofffreie Chalkogenide des Kohlenstoffs (Kohlenstoffmonoxid, Kohlenstoffdioxid, Schwefelkohlenstoff), Kohlensäure und Carbonate, Carbide sowie die ionischen Cyanide, Cyanate und Thiocyanate, Blausäure, Wasserstoffperoxid
• • Stoffe zusammengesetzt aus dem Periodensystem der Elemente
• • Metalle, wie Aluminium, Titan, Eisen, Kupfer
• • Legierungen, wie Bronze
• • Halbleiter
• • Salze
• • Kationen
• • Anionen
• • Liganden
• • Mineralien und Keramiken, wie Silikate
• • Gläser
• • Säuren und Basen, wie Schwefelsäure, Salzsäure, Salpetersäure, Phosphorsäuren, Phosphonsäuren, traust Du Natronlauge, Ammoniak
• • Gase

• Weitere funktionelle Materialien

• • Molekularsiebe
• • Nanopartikel: Aluminiumoxid, Kupfer, Titanoxid, Zinkoxid, Zirkoniumoxid
• • Quantumpunkte und -dots (hydrophob und hydrophil)
• • DC-Platten
• • Elektrophoreseplatten
• • Xylan
• • Öle und Fette
• • Esculin-Sequihydrat
• • Azidoaminosaueren:
• • Fmoc-geschuetze Aziodaminosauere
• • Agarosen
• • Chlorophyll
• • Carotin
• • Abscisinsäure
• • Cyanidin
• • Szintillationscocktail

• Albumin:

• • Albumin Fraktion V
• • Albumin fettsäurefrei
• • Albumin IgG frei
• • Albumin nukleasefrei
• • Albumin endotoxinarm und/oder geprüft
• • Albumin pH 5,2
• • Cetyltrimethylammoniumbromid
• • DEPC (Diethyldicarbonat)
• • Dextran 70, 500
• • Dodecyl-beta-D-maltosid
• • Glycin
• • Guainidn hydrochlorid
• • Guanidinthiocyanant
• • Harnstoff
• • Lecithin
• • Luminol
• • Naphtyl-ethylendiamin dihydrochlorid
• • SDS (Natriumdodecylsulfat), auch als Pellets
• • SDS-PAGE, eine Variante der Polyacrylamid-Gelelektrophorese
• • Thioharnstoff
• • Tris-carboxethyl-phosphin-hydrochlorid

• Reagenzien für die Molekularbiologie

• • Reagenzien zur Beseitigung von RNasen, wie RNase AWAY
• • Amplicillin-Natriumsalz
• • IPTG
• • X-Beta-Gal
• • Nucleinsäurefreie Lösung zur Beseitigung von Nukleinsäueren
• • DNA-Beseitigerlösungen wie DNA AWAY
• • PCR Mixturen
• • dNTP-Sets
• • Ribonuklease A

• Blotting-Papiere und -Membranen
• Histologiereagenzien:

• • Formaldehyd
• • Histofix
• • Osmiumtetroxid
• • Paraffin
• • Mikroskopieloesungen

• Trockenmedien, auch granuliertOrganic:

• • Hydrocarbons without a functional group
• • Halogenated hydrocarbons

• oxygen and hydroxy compounds

• • Alcohols, such as methanol, ethanol, propanol
• • aldehydes, such as formaldehyde
• • esters, such as esters of acetic acid, ethyl lactate
• • Ethers, such as diethyl ether
• Ketones such as isobutyl methyl ketone, acetone, butanone
• • Carboxylic acids, such as acetic acid

Nitrogen compounds

• • Amines, like adamantane
• • Amide
• • Diazonium salts
• • Nitro compounds, for example TNT
• • nitriles

• sulfur compounds

• • Alkane thiols
• • sulphides
• • Disulfides
• • esters of sulfuric acid
• • sulfones
• • Sulfoxides
• • thionamide
• • thiolester
• • thio acid

• phosphorus compounds

• • Ortho-phosphoric acid
• • Phosphoric acid ester
• • phosphines, for example triphenylphosphine

• organometallic compounds

• • ferrocene

• Aliphatic hydrocarbons (aliphatics)

• • Acyclic hydrocarbons
  • ■ saturated (alkanes)
  • ■ unsaturated (alkenes and alkynes)
• • Cyclic hydrocarbons
  • ■ saturated (alkanes)
  • ■ unsaturated (alkenes and alkynes
• • Aromatic hydrocarbons (aromatics)
  • ■ simple aromatics
  • ■ condensed aromatics
• • heterocycles
• • Biochemical compounds
  • ■ alkaloids,
  • ■ amino acids,
  • ■ carbohydrates,
  • ■ proteins,
  • ■ steroids,
  • ■ terpenes,
  • ■ vitamins

• Inorganic substances

• • Hydrogen-free chalcogenides of carbon (carbon monoxide, carbon dioxide, carbon disulfide), carbonic acid and carbonates, carbides and the ionic cyanides, cyanates and thiocyanates, hydrogen cyanide, hydrogen peroxide
• • Substances composed of the periodic table of the elements
• • Metals, such as aluminum, titanium, iron, copper
• • alloys, such as bronze
• • Semiconductors
• • salts
• • Cations
• • anions
• • ligands
• • minerals and ceramics, such as silicates
• • glasses
• • Acids and bases, such as sulfuric acid, hydrochloric acid, nitric acid, phosphoric acids, phosphonic acids, you dare caustic soda, ammonia
• • gases

• Other functional materials

• • molecular sieves
• • Nanoparticles: aluminum oxide, copper, titanium oxide, zinc oxide, zirconium oxide
• • Quantum dots and dots (hydrophobic and hydrophilic)
• • DC plates
• • electrophoresis plates
• • xylan
• • Oils and fats
• • Esculin Sequihydrate
• • azidoamino acids:
• • Fmoc-protected aziodo amino acid
• • agaroses
• • Chlorophyll
• • carotene
• • abscisic acid
• • cyanidin
• • scintillation cocktail

• albumin:

• • Albumin fraction V
• • Albumin fatty acid-free
• • Albumin IgG free
• • Albumin nuclease-free
• • Albumin low in endotoxins and / or tested
• • albumin pH 5.2
• • cetyltrimethylammonium bromide
• • DEPC (diethyl dicarbonate)
• • Dextran 70, 500
• • Dodecyl-beta-D-maltoside
• • Glycine
• • Guainidn hydrochloride
• • guanidine thiocyanate
• • urea
• • lecithin
• • luminol
• Naphtyl-ethylenediamine dihydrochloride
• • SDS (sodium dodecyl sulfate), also as pellets
• • SDS-PAGE, a variant of polyacrylamide gel electrophoresis
• • thiourea
• Tris-carboxy-phosphine hydrochloride

• Reagents for molecular biology

• • Reagents to remove RNases, such as RNase AWAY
• • Amplicillin sodium salt
• • IPTG
• • X Beta Gal
• • Nucleic acid-free solution for the removal of nucleic acids
• • DNA removal solutions such as DNA AWAY
• • PCR mixtures
• • dNTP sets
• Ribonuclease A

• blotting papers and membranes
Histology reagents:

• • formaldehyde
• • Histofix
• Osmium tetroxide
• • paraffin
• • Microscopy solutions

• Dry media, also granulated

Possible multiple mentions of materials are due to multiple functions.

Nutrient media according to the nutritional requirements

minimal medium

It contains the absolutely necessary nutrients for the growth of the microorganism in question. For example, contains these D-glucose as carbon sources, ammonium ions as a nitrogen source and sulfate ions as Schwerfelquelle. The medium is used for the selection and description of a specific microorganism.

The minimal medium - also M-medium or MM - is a nutrient medium, which is used in laboratories mostly for the investigation of the interactions between plants and mycorrhizierenden mushrooms. It was described by G. Becard and J.A. Fortin developed to analyze the exact processes after incipient mycorrhization of a carrot root with the fungus Glomus. It is also widely used in tissue culture experiments where low-nutrient media are required.

In microbiology, minimal media are commonly used to find out what nutrient claims a microorganism has in growth.

Although most nutrient media, such as the commonly used MS medium, provide sufficient nutrients for healthy and fast growth of the plants, the high concentration of sucrose, phosphate and other ingredients prevent germination of the fungal spores. A dual culture of plant and fungus, as well as the investigation of their interactions is therefore not possible. Becard and Fortin have therefore developed a nutrient medium for their experiments, which provides just enough nutrients to keep the plants or plant organs alive while the fungal spores can still germinate and form a mycorrhizating compound with the host.

Ingredients:

• • Magnesiumsulfat (MgSO₄ · 7H₂O) 731 mg/l
• • Kaliumnitrat (KNO₃) 80 mg/l
• • Kaliumchlorid (KCl) 65 mg/l
• • Kaliumdihydrogenphosphat (KH₂PO₄) 4,8 mg/l
• • Calciumnitrat (Ca(NO₃)₂ · 4H₂O) 288 mg/l

macronutrients

• Magnesium sulfate (MgSO ₄ .7H ₂ O) 731 mg / l
• • Potassium nitrate (KNO ₃ ) 80 mg / l
• • Potassium chloride (KCl) 65 mg / l
• • Potassium dihydrogen phosphate (KH ₂ PO ₄ ) 4.8 mg / l
• Calcium nitrate (Ca (NO ₃ ) ₂ .4H ₂ O) 288 mg / l

• • NaFeEDTA 8 mg/l
• • Kaliumiodid (Kl) 0,75 mg/l
• • Manganchlorid (MnCl₂ · 4H₂O) 6 mg/l
• • Zinksulfat (ZnSO₄ · 7H₂O) 2,65 mg/l
• • Borsäure (H₃Bo₃) 1,5 mg/l
• • Kupfersulfat (CuSO₄ · 5H₂O) 0,13 mg/l
• • Natriummolybdat (Na₂MoO₄ · 2H₂O) 0,0024 mg/l

micronutrients

• • NaFeEDTA 8 mg / l
• • potassium iodide (Cl) 0.75 mg / l
• • manganese chloride (MnCl ₂ · 4H ₂ O) 6 mg / l
• Zinc sulfate (ZnSO ₄ .7H ₂ O) 2.65 mg / l
• Boric acid (H ₃ Bo ₃ ) 1.5 mg / l
• Copper sulfate (CuSO ₄ .5H ₂ O) 0.13 mg / l
• Sodium molybdate (Na ₂ MoO ₄ .2H ₂ O) 0.0024 mg / l

• • Glycin 3 mg/l
• • Thiamin 0,1 mg/l
• • Pyridoxin 0,1 mg/l
• • Nicotinsäure 0,5 mg/l
• • Inosit 50 mg/l
• • Agar 10.000 mg/l
• • Saccharose 10.000 mg/l

Organic additives

• • Glycine 3 mg / l
• • thiamine 0.1 mg / l
• • Pyridoxine 0.1 mg / l
• • nicotinic acid 0.5 mg / l
• • inositol 50 mg / l
• • agar 10,000 mg / l
• • sucrose 10,000 mg / l

The optimal pH of the minimal media is 5.5.

complete medium

In addition to the substances in the minimal medium, the complete medium contains growth-promoting substances and supplines. These are e.g. several amino acids as nitrogen source, sulfur-containing amino acids as sulfur source. So it is similar to applications such as the cultivation of numerous microorganisms.

Media according to the workload:

Finished mixtures

In common nutrient media, the solid ingredients together with gelling agents are industrially put together to ensure that the composition always remains uniform. Such ready mixes are available as powder, granules, tablets or lyophilisate. If necessary, these ready-made mixtures need only be dissolved in heated water and sterilized.

Fertigmediem

In the finished medium, the medium has already been industrially produced and filled into petri dishes or tubes. The finished medium can be used directly without further preparation.

Media according to the study objective:

Media are also used to select microorganisms based on particular properties. There are two approaches

selective media

Selective media only allow the growth of certain microorganisms that have particular properties to proliferate in this medium. An example is media that are fortified with antibiotics. In them only those microorganisms can grow that are resistant to the antibiotic used.

differential media

Differential media (also called differentiation media or indicator nutrient media) allow the growth of several microorganisms used. However, they are so composed that the resulting colonies of the various microorganisms differ in appearance from each other so that they can be differentiated. For example, on Blood Agar, bacterial strains capable of hemolysis can be distinguished from others by the clearing-house around their colonies.

A well-known medium that combines both of these principles is the MacConkey agar. It contains bile salts and crystal violet and thus prevents the growth of Gram-positive bacteria and thus acts as a selective medium. For its action as a differential medium lactose and neutral red is included, so that lactose-fermenting bacteria can be identified by a color change of the pH indicator neutral red. Another commonly used culture medium that acts as both a selective medium and a differential medium is the XLD agar.

Media for Replica Plating:

Replica plating refers to the transfer of all colonies from one solid nutrient medium to another solid nutrient medium to obtain the alignment of the individual colonies. For this purpose, a sterile velvet cloth is stretched on a Lederberg stamp, which is first applied to a plate covered with colonies and then to an unvegetated plate Pressing is transferred. The impression produces a copy of the colonies on the solid nutrient medium which is cultured to grow the colonies. The pile fibers of the velvet avoid smearing the colonies. Prior to using velvet, transfer of colonies was done with sterile toothpicks or with sterile filter paper.

Examples of the media:

• ➢DMEM (Well supported for the growth of a spectrum of mammalian cell lines.)
• ➢ DMEM / F-12 (1:1 Mix of DMEM and Ham's F-12)
• ➢KnockOut DMEM
• ➢ DMEM/F-12 Und besonders
• ➢ KnockOut™ D-MEM/F-12
• ➢ Opti-MEM medium (Ideal for use during cationic lipid transfections.)
• ➢DMEM, high glucose, GlutaMAX™ Supplement
• ➢ DMEM, high glucose, GlutaMAX™ Supplement, HEPES
• ➢DMEM, high glucose, GlutaMAX™ Supplement, pyruvate
• ➢DMEM/F-12, GlutaMAX™ supplement

Dulbecco's Minimum Essential Media: Dulbecco's Modified Eagle Medium

• ➢DMEM (Well supported for the growth of a spectrum of mammalian cell lines.)
• ➢ DMEM / F-12 (1: 1 Mix of DMEM and Ham's F-12)
• ➢KnockOut DMEM
• ➢ DMEM / F-12 And especially
• ➢ KnockOut ™ D-MEM / F-12
• ➢ Opti-MEM medium (Ideal for use during cationic lipid transfections.)
• ➢DMEM, high glucose, GlutaMAX ™ Supplement
• ➢ DMEM, high glucose, GlutaMAX ™ Supplement, HEPES
• ➢DMEM, high glucose, GlutaMAX ™ Supplement, pyruvate
• ➢DMEM / F-12, GlutaMAX ™ supplement

• ➢ MEM
• ➢ MEM (Patterned after Eagle's Media, well suited for mammalian cells.)
• ➢MEM α, GlutaMAX™ Supplement, no nucleosides
• ➢MEM α, nucleosides, GlutaMAX™ Supplement
• ➢ GlutaMAX™ Supplement
• ➢ MEM, GlutaMAX™ Supplement
• ➢ MEM, HEPES, GlutaMAX™ Supplement
• ➢Opti-MEM™ Reduced Serum Medium, GlutaMAX™ Supplement

Iscove's Modified Dubelcco Media

• ➢ MEM
• MEM (Patterned after Eagle's Media, well suited for mammalian cells.)
• ➢MEM α, GlutaMAX ™ Supplement, no nucleosides
• ➢MEM α, nucleosides, GlutaMAX ™ Supplement
• ➢ GlutaMAX ™ Supplement
• ➢ MEM, GlutaMAX ™ Supplement
• ➢ MEM, HEPES, GlutaMAX ™ Supplement
• ➢Opti-MEM ™ Reduced Serum Medium, GlutaMAX ™ Supplement

IMDM (Highly enriched synthetic media, well suited for proliferating, high-density cell cultures)

• ➢ Medien RPMI 1640 (Enriched media with extensive applications for mammalian cells.)
• ➢ RPMI 1640 Medium, GlutaMAX™ Supplement
• ➢ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

RPMI (Roswell Park Memorial Institute) media

• ➢ Media RPMI 1640 (Enriched media with extensive applications for mammalian cells.)
• ➢ RPMI 1640 Medium, GlutaMAX ™ Supplement
• ➢ RPMI 1640 Medium, GlutaMAX ™ Supplement, HEPES

• ➢ GlutaMAX media
• ➢ HAM's
• ➢ HAMs F-12
• ➢ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement
• ➢ IMDM, GlutaMAX™ Supplement

specialty media

• ➢ GlutaMAX media
• ➢ HAM's
• ➢ HAMs F-12
• Ham's F-12 Nutrient Mix, GlutaMAX Supplement
• ➢ IMDM, GlutaMAX ™ Supplement

• ➢ Williams' Media E
• ➢CMRL
• ➢ Glasgow (G-MEM)
• ➢ Improved MEM
• ➢Modified Eagle Medium (MEM)
• ➢BME
• ➢CO₂-Independent Medium
• ➢ Waymouth's MB 752/1)
• ➢BGJb (Fitton-Jackson Modification)
• ➢Brinster's BMOC-3

McCoy's 5A

• ➢ Williams' Media E
• ➢CMRL
• ➢ Glasgow (G-MEM)
• ➢ Improved MEM
• ➢Modified Eagle Medium (MEM)
• ➢BME
• ➢CO ₂ -Independent medium
• ➢ Waymouth's MB 752/1)
• ➢BGJb (Fitton-Jackson Modification)
• ➢Brinkster's BMOC-3

MCDB 131
MEM Regar 3
Fischer's medium
NCTC-135
Leibovitz's L15
McCoy's 5A
Medium 199 / Earle's
TC100
William's E

• ➢ DMEM Media
• ➢ Minimum Essential Media (MEM)
• ➢ RPMI Medium 1640
• ➢ DMEM/F-12 Media
• ➢ Media 199
• ➢ F-12 Nutrient Mixture
• ➢ Iscove's (IMDM)

Mammalian classical media

• ➢ DMEM Media
• ➢ Minimum Essential Media (MEM)
• ➢ RPMI Medium 1640
• ➢ DMEM / F-12 Media
• ➢ Media 199
• ➢ F-12 Nutrient Mixture
• ➢ Iscove's (IMDM)

• • Sf9 & Sf21 Cell Culture
• • High Five™ Cell Culture
• • Drosophila S2 Cell Culture

Reduced-serum media
Cytogenetics media
Bioproduction media
PSC (pluripotent stems cells) Neural Induction Medium
MesenPRO RS™ Medium
DMEM/F-12, HEPES
Ham's F-12K (Kaighn's) Medium
Brinster's, No Glutamine, No Phenol Red
StemPro™-34 SFM (1X)
PFHM-II Protein-Free Hybridoma Medium
RPMI 1640 Medium, GlutaMAX
Essential 8
Oxoid Saline
Medium 199
DMEM, High Glucose, No Glutamine, No Methionine, No Cystine
MEM, Suspension, No Calcium, No Glutamine
Intestinal Epithelium Differentiation Medium
Hibernate™-E MediumMammalian protein expression media
Hybridoma media
Primary cell media
Stem cell media
Neurobiology media
Insect media

• • Sf9 & Sf21 Cell Culture
• • High Five ™ Cell Culture
• • Drosophila S2 cell culture

Reduced-serum media
Cytogenetics media
Bioproduction media
PSC (Pluripotent Stems Cells) Neural Induction Medium
MesenPRO RS ™ Medium
DMEM / F-12, HEPES
Ham's F-12K (Kaighn's) medium
Brinster's, No Glutamine, No Phenol Red
StemPro ™ -34 SFM (1X)
PFHM-II Protein-Free Hybridoma Medium
RPMI 1640 Medium, GlutaMAX
Essential 8
Oxoid Saline
Medium 199
DMEM, High Glucose, No Glutamine, No Methionine, No Cystine
MEM, Suspension, No Calcium, No Glutamine
Intestinal Epithelium Differentiation Medium
Hibernate ™ -E medium

Auxiliary media:

• o DPBS with and without EDTA
• o PBS with and without EDTA
• o EDTA
• ◯ trypsin
• ◯ trypsin inhibitor

• ◯ Ficoll 400
• ◯ Polysucrose 400
• ◯ Sep 1077

Media for cell isolation agents

• ◯ Ficoll 400
• ◯ Polysucrose 400
• 10 Sep 1077

• ◯ DOTAP
• ◯ DEAE Dextran
• ◯ Fect RNAi und der Kit
• ◯ Fect Plus
• ◯ Insectofect

Media with transfection agent

• ◯ DOTAP
• DEAE Dextran
• ◯ Fect RNAi and the kit
• ◯ Fect Plus
• ◯ Insectofect

• ◯ Neutralrot
• ◯ Loefflers Methylenblauloesung und Methylenblauloesung
• ◯ Tryphanblau, besonders (C.I. 23850)
• ◯ Testloesungen zur Bestimmung der Zellproliferation wie Rotitest Vital

Media for cell analysis, such as vitality assay and cell counting

• ◯ neutral red
• ◯ Loefflers Methylene Blue Leaching and Methylene Blue Leaching
• ◯ Tryphan blue, especially (CI 23850)
• ◯ Test solutions for determining cell proliferation such as Rotitest Vital

• ◯ Imaging
• ◯ Flow Cytometry

Media for proliferation assays for

• ◯ Imaging
• C flow cytometry

• ◯ 5-Brom-2'-desoxyuridin
• ◯ PBST
• ◯ Tween 20, und andere Tween
• ◯ Histofix
• ◯ DAPI
• ◯ Methanol

Media for High Throughput Screening

• 5-bromo-2'-deoxyuridine
• ◯ PBST
• ◯ Tween 20, and other tween
• ◯ Histofix
• ◯ DAPI
• ◯ methanol

• ◯ Annexin V
• ◯ PBS
• ◯ Blockierung
• ◯ ImmunoBlock
• ◯ Albunim
• ◯ Albumin, IgG-frei

apoptosis assay

• ◯ Annexin V
• ◯ PBS
• ◯ blocking
• ◯ ImmunoBlock
• ◯ Albunim
• ◯ albumin, IgG-free

• ◯ BCIP und das Natriumsalz
• ◯ BCIP-Touidinsalz
• ◯ NBT
• ◯ Rose-phos-Toluidinsalz
• ◯ DAB
• ◯ Phosphatase
• ◯ Meerrettisch-Peroxidase

Media for immunodetections

• ◯ BCIP and the sodium salt
• ◯ BCIP touidine salt
• ◯ NBT
• ◯ Rose-phos-toluidine salt
• ◯ DAB
• ◯ Phosphatase
• ◯ Horse radish peroxidase

• ◯ DPBS/EDTA mit und ohne Ca/Mg
• ◯ PBS/EDTA
• ◯ Trypsin, z.B. BSE und oder TSE frei, auch salzfrei
• ◯ Trypsin Inhibitor wie BAEE, z.B. salzfrei

Media for cell passage

• ◯ DPBS / EDTA with and without Ca / Mg
• ◯ PBS / EDTA
• ◯ Trypsin, eg BSE and / or TSE free, also salt-free
• ◯ Trypsin inhibitor like BAEE, eg salt-free

• ◯ Ficoll 400
• ◯ Polysucrose 400
• ◯ Sep 1077

Media for cell isolation

• ◯ Ficoll 400
• ◯ Polysucrose 400
• 10 Sep 1077

Media for degradation ◯ Tween 20, 40 etc.

Media for analysis

• ◯ 2x YT Agar
• ◯ 2x YT Medium, auch granuliert
• ◯ Acetamid Naehrloesung
• ◯ Antibioktikamedium, auch nummer 23
• ◯ Antibitikamedium, Nr. 1
• ◯ Azid-Glucose-bouillon
• ◯ Baird-Parker Agar
• ◯ Bengalrot-Agar
• ◯ Bengalrot-Agar, auch mit chloramphinikol
• ◯ Blutagar, auch Nr. 2
• ◯ Brilliantgrün-Galle-Medium
• ◯ Brilliantgruen-Galle-Thinat-Bouillion
• ◯ Brilliantgruen-Lactose-Saccarose-Agar
• ◯ Brucella Agar, und Bouillon
• ◯ Brucella-Agar
• ◯ Campylobacter-Agar
• ◯ Campylobacter-Agar, auch blutfrei
• ◯ Candidachromogener Agar
• ◯ CASO Agar, mit Soy, Broth, TSB, Lecitin, Polysorbat 80, auch alles als bouillon
• ◯ CASO-Agar
• ◯ CASO-Bouillon, auch gruliert
• ◯ Cetriid Agar
• ◯ CLED Agar
• ◯ Clostridien-Anreicherungsmedium
• ◯ Clostridien-Differential-Bouillon
• ◯ Coli-fluoro-Medium
• ◯ Coliforme chromogener Agar
• ◯ Columbia-Agar
• ◯ Cronobacter chromogener Agear
• ◯ D/E-Neutralisierungs Agar
• ◯ Desxoxylcholat Citrat Agar
• ◯ Detrimid-Agar
• ◯ Dichloran-Bengalrot-Agar
• ◯ Dichloran-glycering-Agar
• ◯ DNase-Test Agar
• ◯ EC Bouillon
• ◯ Endo-Agar
• ◯ Fraser-Halb-Medium
• ◯ Galle-Esculin-Azid Medium
• ◯ Gelantine Agar
• ◯ Harnstoffagar nach Christinaxen
• ◯ Hefe-Stickstoff-Medium
• ◯ Hefeextraktagar
• ◯ Hefel-Glucose Agar
• ◯ Hirn-Herz-Glucose-bouilon
• ◯ Kartoffelextrakt-Glucose Agar
• ◯ King's B Medium
• ◯ Klinger-Eisen-Agar
• ◯ Kristallviolett-Galle- Agar mit glucose und Lactose
• ◯ Kristallviolett-Galle-glucose Agar
• ◯ Lacotse-sulfit Medium
• ◯ Lactose Bouillon
• ◯ Lactose TTC Agar
• ◯ Lactose-Sulfit-Medium
• ◯ Laurylsulfat-Bouillon
• ◯ LB Agar und Medium, auch granuliert und vegetal
• ◯ Legionella-Agar
• ◯ Letheen Agar und Bouillon
• ◯ Listeria chromogener Agar
• ◯ Lysin Eisen Agar
• ◯ m-CP-Agar
• ◯ m-EI-chromogener Agar
• ◯ MacConkey-Agar
• ◯ MacConkey-Bouillon
• ◯ Malzextrakt Agar und Bouillon
• ◯ Mannit-Kochsalz Agar
• ◯ Mannitol-Eigelb-Polymyxin Agar
• ◯ Marine Bouillon
• ◯ Maximal-Wiederbelebungsloesung
• ◯ Mossel-Anreicherungsmedium
• ◯ MRS-Agar, auch pH 5,7, und chromogener
• ◯ MRS-Bouillon
• ◯ Müller-Hinton Agar und Bouillon
• ◯ Müller-Kaufmann-bouillon
• ◯ Naehragar auch DEV/ISO und DEV und bouillon
• ◯ NZCYM Medium
• ◯ NZYM Medium
• ◯ Organgenserum Agar
• ◯ Oxford-Listeria-Agar
• ◯ Palcalm-Listeria Agar
• ◯ Peptonwasser, gepuffert und auch granuliert
• ◯ Plate-count Agar, auch mit Magermilch
• ◯ Pseudomonas-Selektiv-Agar und chromogener
• ◯ R2A Agar
• ◯ Rappaport-Vassiliadis-Bouillion
• ◯ Rogosa Agar
• ◯ Sabouraud 2% Glucose Bouillon
• ◯ Sabouraud-4%-Glucoase Agar, auch mit Chloramphinicol
• ◯ Salmonella chigella Agar
• ◯ Salmonelle chromogener Agar
• ◯ Schaedler Agar und bouillon
• ◯ Simmons Citrat Agar
• ◯ Slanetz Bartley Medium und Agar, auch mit Tetazoliumchlorid
• ◯ SOB Medium
• ◯ Sorbitol-MacConkey-Agar
• ◯ Standard Keimzahlagar
• ◯ Standard Nähragar und Nährmedium
• ◯ TAT Bouillon
• ◯ TBX chromogener Agar
• ◯ TCBS Agar
• ◯ Terrific Broh, auch modifiziert oder vegetal
• ◯ Todd-Hewitt bouillon
• ◯ Tripel-Zucker-eisen-Agar
• ◯ TSC Agar
• ◯ Universal Bier Agar
• ◯ UTI chomogener Agar
• ◯ Würzbouillon
• ◯ Würzeagar
• ◯ XLD Agar
• ◯ Yersinia Selektirv Agar
• ◯ YPD Agar und Medium
• ◯ Tryphan-soja Agar
• ◯ Thyphan Bouillon und Agar
• ◯ Tryphon-Galle-Agar und Bouillon

Media in microbiology, partly according to the European Pharmacopoeia

• ◯ 2x YT agar
• ◯ 2x YT medium, also granulated
• ◯ Acetamide nutrient solution
• ◯ antibiotic medicine, also number 23
• ◯ Antibassy Media, No. 1
• ◯ Azide-glucose broth
• ◯ Baird-Parker Agar
• ◯ Bengal red agar
• ◯ Bengal red agar, also with chloramphinikol
• ◯ Blood Agar, also No. 2
• ◯ Brilliant green bile medium
• ◯ Brilliant-green-bile-thinat-Bouillion
• ◯ Brilliant green lactose-saccarose agar
• ◯ Brucella Agar, and bouillon
• ◯ Brucella agar
• ◯ Campylobacter agar
• ◯ Campylobacter agar, also free of blood
• ◯ Candidachromogener agar
• ◯ CASO agar, with soy, broth, TSB, lecithin, polysorbate 80, also everything as a bouillon
• ◯ CASO agar
• ◯ CASO broth, also grumbled
• ◯ Cetriid Agar
• ◯ CLED agar
• ◯ Clostridium enrichment medium
• ◯ Clostridia differential broth
• ◯ Coli-fluoro medium
• ◯ Coliform chromogenic agar
• ◯ Columbia agar
• ◯ Cronobacter chromogenic agar
• ◯ D / E neutralization agar
• ◯ Desxoxylcholate Citrate Agar
• ◯ Detrimid agar
• ◯ Dichloran-Bengal red agar
• ◯ Dichloran glycerol agar
• ◯ DNase test agar
• ◯ EC broth
• ◯ Endo-agar
• ◯ Fraser half-medium
• ◯ bile esculin azide medium
• ◯ Gelatin Agar
• ◯ Urea agar after Christinaxen
• ◯ yeast nitrogen medium
• ◯ Yeast extract agar
• ◯ yeast-glucose agar
• ◯ brain-heart-glucose-bouilon
• ◯ Potato extract-glucose agar
• ◯ King's B Medium
• ◯ Klinger iron agar
• Crystal violet bile agar with glucose and lactose
• Crystal violet-bile-glucose agar
• ◯ Lacotse sulfite medium
• ◯ Lactose Bouillon
• ◯ Lactose TTC agar
• ◯ Lactose sulphite medium
• ◯ Lauryl sulfate broth
• ◯ LB agar and medium, also granulated and vegetal
• ◯ Legionella agar
• ◯ letheen agar and bouillon
• ◯ Listeria chromogenic agar
• ◯ lysine iron agar
• -M CP agar
• -M-EI chromogenic agar
• ◯ MacConkey agar
• ◯ MacConkey Broth
• ◯ Malt extract agar and bouillon
• ◯ mannitol salt agar
• ◯ Mannitol egg yolk polymyxin agar
• ◯ Marine Bouillon
• ◯ Maximum Resuscitation Solution
• ◯ Mossel enrichment medium
• ◯ MRS agar, also pH 5.7, and chromogenic
• ◯ MRS broth
• ◯ Müller-Hinton agar and bouillon
• ◯ Müller-Kaufmann broth
• ◯ Naehragar also DEV / ISO and DEV and bouillon
• ◯ NZCYM medium
• ◯ NZYM medium
• ◯ organogen serum agar
• ◯ Oxford Listeria agar
• ◯ Palcalm-Listeria Agar
• ◯ Peptone water, buffered and also granulated
• ◯ Plate-count agar, also with skimmed milk
• ◯ Pseudomonas selective agar and chromogenic
• ◯ R2A agar
• ◯ Rappaport-Vassiliadis-Bouillion
• ◯ Rogosa Agar
• ◯ Sabouraud 2% Glucose Broth
• ◯ Sabouraud 4% glucoase agar, also with Chloramphinicol
• Salmonella chigella agar
• Salmonella chromogenic agar
• ◯ Schaedler agar and bouillon
• ◯ Simmons Citrate Agar
• ◯ Slanetz Bartley medium and agar, also with tetazolium chloride
• ◯ SOB medium
• ◯ Sorbitol MacConkey agar
• ◯ Standard germ count agar
• ◯ Standard nutrient agar and nutrient medium
• ◯ TAT broth
• ◯ TBX chromogenic agar
• ◯ TCBS agar
• ◯ Terrific Broh, also modified or vegetal
• ◯ Todd-Hewitt bouillon
• ◯ Triple sugar iron agar
• ◯ TSC Agar
• ◯ Universal Beer Agar
• ◯ UTI chomogenic agar
• ◯ Seasoning broth
• ◯ Würzeagar
• ◯ XLD agar
• ◯ Yersinia Selektirv agar
• ◯ YPD agar and medium
• ◯ Tryphan-soya agar
• ◯ Thyphan Bouillon and Agar
• ◯ Tryphon bile agar and bouillon

Examples of important cell cultures are listed in Table 2. Table 2: Cultures of human and animal cells 10B human Endometriotic cells 11Z, 11E human Endometriotic cells 1273/99 (1273) human synovial sarcoma 12Z human Endometriotic cells 1321N1 human brain 1411H human teratocarcinoma 143B human osteosarcoma 174xCEM human Hybrid between human B and T cell line 17B human Endometriotic cells 17cl-1 mouse fibroblasts 18B human Endometriotic cells 1 BR.3.GN human 1C11 mouse teratocarcinoma 20-1 human hepatoma 20B human Endometriotic cells 21-5 human hepatoma 22B human Endometriotic cells 22Rv1 human prostate cancer 25Z human Endometriotic cells 266-6 mouse pancreatic cells 293 (HEK 293) human kidney 293 GPG human kidney 293 TVA800 human kidney 293A (HEK 293A) human kidney 293FT human kidney 293LTV human kidney 293T (HEK 293T) human kidney 293T-Rex human kidney 293T / 17 human kidney 293TGPRT + R1 human kidney 293TT (HEK 293TT) human kidney 2FTGH human fibrosarcoma 2H-11 mouse epithelial cells 3, 4 human Endometriotic cells 300.19 mouse B-cell lymphoma 32D mouse bone marrow 33Z human Endometriotic cells 38C13 mouse B-cell lymphoma 39Z human Endometriotic cells 3A (tPA 30-1) human placenta 3D4 pig macrophages 3LL A9 mouse lung cell 3T3-L1 mouse fibroblasts 3T3-Swiss albino mouse Swiss albino mouse fibroblasts 40Z human Endometriotic cells 42B human Endometriotic cells 45Z human Endometriotic cells 49Z human Endometriotic cells 4T1 mouse mammary tumor 50Z human Endometriotic cells 55Z human Endometriotic cells 5637 human 57z-T1 human Endometriotic cells 57z-T2 human Endometriotic cells 721220 human B-cell 721220-B4402 human B-cell 721220-B4405 human B-cell 85HG66 human 8E5 human T cell 9-4Z human Endometriotic cells 9-8Z human Endometriotic cells 911 human retinoblasts A-431 human Squamous cell carcinoma A-498 human kidney A-9 L mouse fibroblasts A2780 human ovarian cancer A2780cis human ovarian cancer A3.01 human T-Lymphoblastomzelllinie A375 human Epithelial cells, malignant melanoma A375M human melanoma A3R5 human T-Lymphoblastomzelllinie A549 human Lung carcinoma, adenocarcinoma (lung) A818-4 human Adenoma (pancreas) ABE-8.1 / 2 mouse B-cell lymphoma ACH-2 human T cell AGS human Adenocarcinoma (stomach) alphaTC1 clone 6 mouse Adenoma (pancreas) alphaTC1 clone 9 mouse Adenoma (pancreas) AM C6SC8 pig AML-12 mouse AmphoPack-293 human kidney ANJOU 65 human kidney ARPE-19 human As4.1 mouse kidney AsPC-1 human pancreatic cancer AT1 mouse cardiomyocytes ATDC5 mouse chondrocytes AtT-20 mouse brain B-3 human lens epithelium B-CPAP human thyroid carcinoma B-LCL human B-cell B16 mouse melanoma B16-F10 mouse melanoma B16V mouse melanoma B51LiM mouse Colon carcinoma B7GG hamster kidney B95-8 monkey B-cell B95a monkey B-cell Ba / F3 mouse asked bat lung epithelium BC-1 human B-cell lymphoma BC-2 human B-cell lymphoma BC-3 human B-cell lymphoma BEAS-2B human human bronchial epithelial cells, NHBE, (normal bronchial epithelium cells) beta TC6 mouse pancreatic cancer BFH12 beef hepatocytes Bra 24 Rabbits Lymphocyte Bhas 42 mouse fibroblasts BHK-21 / BHK-21 / BHK-21CI13 hamster kidney BHK ENVA hamster kidney BHK T7 hamster kidney BJAB human Burkitt's Lymphoma BL-2 human Burkitt's Lymphoma BL-3 beef B-cell lymphoma BL-41 human Burkitt's Lymphoma BL-60 human B-cell lymphoma BL-70 human Burkitt's Lymphoma BOMAC beef macrophages BOS2 mouse neuroblasts BOSC 23 human kidney bovine colonocytes beef Colon bovine jejunocytes beef jejunum BRL-3A rat broli human Merkel cell carcinoma BS-C-1 monkey kidney BSC40 monkey BSR-T7 / 5 hamster kidney BSR-VSV-RV G hamster kidney BT-549 human breast carcinoma BT054 human glioblastoma BT088 human glioblastoma BT474 human Adenocarcinoma (breast) BT549 human BV-2 mouse Microglia, murine dendritic cell line BxPC3 human pancreatic cancer BXS0116 human bone marrow BYS0112 human bone marrow C-20 / A4 human chondrocytes C-33A human cervical cancer C-per-Lu bat lung epithelium C1, C2 mouse Myoblast, musculature C127I mouse mammary tumor C1R-neo human C2C12 mouse Myoblast, musculature C3H / 10T1 / 2 mouse embryo C4-1 human cervical cancer C4-II human cervical cancer C6 rat glioblastoma C6 / 36 insect C8161 human melanoma C8166 human Lymphocyte C8166 SEAP human Lymphocyte CA-HPV-10 human prostate cancer Caco-2, CaCO2 human adenocarcinoma Cad2 mouse Adenocarcinoma (breast) Cal33 human epithelial cells Calu-3 human Adenocarcinoma (lung) CAP human Capan-1 human pancreatic cancer Capan-2 human pancreatic cancer CaPi carp pituitary CARLU / 1 bat lung cell CaSki human cervical cancer CB-5-7-1 human B-cell CB5-B8 human B-cell CB6-16 human B-cell CB6-3 human B-cell CCB carp brain CCL13 human cervical cancer CD34 + human stem Cells CEC 32 chicken embryonic fibroblasts CEC-32 / chMx-Luc chicken embryonic fibroblasts CEF chicken primary embryonic chicken fibroblasts CEM human T cell CEM-GFP human T cell CEM-NKR human T-Lymphoblastomzelllinie CEM-T4 human T cell CEMx174 human Hybrid between human B and T cell line CEMx174 SEAP human Hybrid between human B and T cell line CEMxSS human T cell cend mouse brain capillaries Cf2Th dog thymus CFBE CFTR-mCherry-Flag-wt / F508del human bronchial CFBE CFTR-wt / F508del human bronchial CFBE410- human bronchial CHCC OU2 chicken fibroblasts CHME-5 human CHO hamster ovaries CHO-K1 hamster ovaries CHO / DHFR hamster ovaries cholangiocytes mouse epithelial cells CHON-001 human CHON-002 human XES-214 salmon embryo CIN-612 9E human Cervical carcinoma / epidermal carcinoma Ciptec human kidney CME-1 human synovial sarcoma CMK human acute megakaryocytic leukemia CMMT monkey mammary tumor CMT-64 mouse Adenocarcinoma (lung) Colo201 human Colon cancer Colo205 human Colon carcinoma Colo320 human colon cancer COLO320DM human colon cancer Colo357 human pancreatic cancer Colo829 human melanoma COS, COS-1, COS-7 monkey kidney COV362 human ovarian cancer CP-A (KR-42421) human esophagus CP-B (CP-52731) human esophagus CP-C (CP-94251) human esophagus CP-D (CP-18821) human esophagus CR duck duck embryo CRE mouse fibroblasts CRFK cat kidney CRIP mouse fibroblasts CRO-AP2 human B-cell lymphoma CRO AP3 human B-cell lymphoma CRO-AP5 human B-cell lymphoma CRO-AP6 human B-cell lymphoma CT26 mouse Colon carcinoma CTAC dog adenocarcinoma Ctrl-1 human fibroblasts CV1, CV-1 monkey kidney D-17 dog osteosarcoma D10.G4.1 mouse Lymphocyte D341 human medulloblastoma DAMI human megakaryoblasts Daudi human B-cell lymphoma DB human B-cell lymphoma DBS FRhL-2 monkey lung cell DC2.4 mouse bone marrow Detroit 562 human pharyngeal DEV human lymphoma DF-1 chicken fibroblasts DF-1 / chIL28RA chicken fibroblasts DG-75 human Burkitt's Lymphoma DKN1 dolphin kidney DLD-1 human Adenocarcinoma (colon) DOHH-2 human B-cell lymphoma DT40 chicken B-cell DU145 human prostate cancer E.G7-OVA mouse lymphoma EA. hy926 human endothelial EcoPack2TM-293 human kidney ECV304 human bladder cancer Efnb2-lox / lox, Efnb2-KO mouse epithelial cells EHEB human B-cell lymphoma EidNi / 41 flying Fox kidney EKVX human lung cancer EL4 mouse T cell elona Syrian hamster tumor EML-3C horse macrophages Endoc-ssh1 human pancreatic cells Endoc-ssh2 human pancreatic cells Endoc-SSH3 human pancreatic cells EOC 13.31 mouse brain EPC carp epithelial cells EPC1 hTERT human esophagus EPC2 hTERT human esophagus EpH4 mouse immortalized murine mammary epithelial cells EpoNi / 22.1 bat kidney ES-2 human ovarian cancer F-11 Mouse / rat brain F98npEGFRvlll rat FaDu human epithelial cells FAMPAC human Adenocarcinoma (stomach) FAO rat hepatocytes Fcwf-4 cat macrophages FeT-1C cat Mononuclear cells (PMBC), T lymphocytes FHM Minnow lat .: Phoxinus phoxinus epithelial cells FLC-4 human hepatoma FLM-R beef Flotzmaul Flp-In-293 human kidney Flp-In BHK hamster kidney Flp-In CV1 monkey kidney Flp-in Jurkat human T cell FMH202-1 mouse hepatocytes FRhK-4 monkey kidney FS-1 human testicle FU DDLS-1 human liposarcoma Fuji human synovial sarcoma G-292 Clone A141B1 human G44 human glioblastoma g62 human glioblastoma GaLV mouse fibroblasts gb0864 monkey lymphoblast GC-2SPD (ts) mouse spermatocytes GC-LCL human B-cell Ghost human osteosarcoma Gibco episomal hiPSC Line human stem Cells Gic 1-5 human glioblastoma GLUTag mouse intestine GM-95 mouse GP + E 86 mouse fibroblasts GP + E AM12 mouse fibroblasts GP2-293 human kidney GPC-16 Guinea pig Adenocarcinoma (colon) GPNT rat brain Granta 519 human B-cell lymphoma H-4-II-E rat hepatocytes H1299 human human non-small cell lung carcinoma H441 human Adenocarcinoma (lung) H6c7 human epithelial cells H9 human T cell H9c2 (2-1) rat Adenocarcinoma (breast) H9puroFF human T cell HaCat human keratinocyte HaCaTII4 human epithelial cells Hank-92 human natural killer cells HAP1 human Mononuclear cells (PMBC), T lymphocytes HB-8065 human hepatocytes HBEC-5i human endothelial HBEpC-c human epithelial cells HBL-1 (Abe and Nowaza) human B-cell lymphoma HBL-1 (Gaidano) human B-cell lymphoma HBL-100 human breast epithelium HBL-2 human B-cell lymphoma HBMEC (Large T antigen immortalized) human brain HBT-43 human pharyngeal HCC-2998 human colon cancer HCC-78 human Adenocarcinoma (lung) HCE-T (Araki-Sasaki) human cornea HCE-T (barge) human cornea HCEC CT1 human HCEC CT2 human HCK human cornea HCMC / D3 human endothelial hCMEC / D3 human brain HCN-2 human neural tissue HCT 116 human Colon cancer HCT-15 human Adenocarcinoma (colon) HCT-8 human Adenocarcinoma (colon) HDLM-2 human lymphoma HEK-293T CD63-GFP human kidney HEK293S GnTI human kidney HEL human erythrocytes HEL (92.1.7) human lymphoblast HEL 299 human fibroblasts HeLa human cervical cancer HeLa S3 human cervical cancer HeLa-CD4 human cervical cancer HeLa EM2 human HeLa Fucci human HeLa-tat III human cervical cancer HeLa229 human cervical cancer HeLaT (clone-4) human cervical cancer HEp-2 human Laryngeal carcinoma Hep-56.1b; Hep-56.1d mouse liver Hep3B human hepatocytes HEPA1-6 mouse hepatoma HepAD38 human hepatocytes HepaRG human hepatoma HepG2 human hepatocytes HepG2 H1.3 human hepatocytes HepG2 H1.3dx human hepatocytes HepG2-2.2.15 human Liver, hepatocytes HepG2-3.22 human hepatocytes HepG2-4A5 human liver HepG2.117 human hepatocytes HepG2.TA2-7 human hepatocytes HER2-tank human natural killer cells HES-2 human embryonic fibroblasts HES-3 human embryonic fibroblasts HEY human ovarian cancer HFF human hFOB 1.19 human osteoblasts High Five (BTI-TN-5B1-4) insect hiPSC A18945 human cord blood HIT-T15 hamster pancreatic cells HK-2 human kidney HKB 11 human kidney HKC-8 human kidney HL-1 mouse HL-60 human Acute myeloid leukemia cells HLE human hepatoma HLF human hepatoma HMC-1 human mast cells HMEC-1 human endothelial hMSC-tert human stem Cells HNEpC-c human epithelial cells HNSC.100 human neural stem cells HNSC.Lat human HOP-62 human Adenocarcinoma (lung) HOP-92 human human non-small cell lung carcinoma HOS human osteosarcoma TROUSERS 17.1 human epithelial cells TROUSERS 6.3 human epithelial cells HOXB8-FL mouse bone marrow HpL3-4 mouse brain HPMEC-ST1.6R human lung HRT-18 human Adenocarcinoma (colon) HS-27A human Bone marrow (stroma) HS-5 human Bone marrow (stroma) HS-SY-II human synovial sarcoma Hs1.Tes human testis Hs578T human Adenocarcinoma (breast) HSB-2 human Mononuclear cells (PMBC), T lymphocytes HSC-F monkey T cell HT-1080 human fibrosarcoma HT-22 mouse neural tissue HT-29 human colon cancer HT-4 mouse neural tissue HT-STAR human fibrosarcoma HTEpC-c human epithelial cells hTERT RPE-1 human retina hTERT HME1 human breast epithelium HTR-8 / SVneo human trophoblast HUES 2 human embryonic fibroblasts HUES 8 human embryonic fibroblasts Huh6 human hepatoma HuH7 human hepatocytes Huh7 Luned human hepatoma Huh7.1 human Huh7.5 human hepatoma HAT 78 human T cell HUVEC human endothelial HypNi / 1.1 flying Fox kidney I3 (TE03) human stem Cells IB3-1 human human bronchial epithelial cells, (cystic fibrosis) IC 21 mouse macrophages IDE2 tick embryo IDE8 tick embryo IDG-SW3 mouse osteocytes IEC-6 rat IGR-OV1 human Adenocarcinoma (ovary) IMR-5 human neuroblastoma IMR90-1 human embryonic fibroblasts In-R1-G9 hamster Insulinom INA-6 human B-cell iNGN hiPSC human stem Cells INS-1E rat pancreatic cells IRE11 tick embryo IRE19 tick embryo IRE20 tick embryo ISE18 tick embryo ISE6 tick embryo J-Lat 10.6 human T cell J-Lat 6.3 human T cell J-Lat 8.4 human T cell J.RT-T3.5 human J111 human macrophages J558L mouse myeloma cells J774 mouse macrophages J82 human bladder cancer Jurkat human T cell Jurkat -1G5 human T cell Jurkat E6 human T cell Jurkat E6-1 human T cell Jurkat-tat human T cell JVM-2 / -3 human B-cell lymphoma K-562 human chronic myeloid leukemia K4IM human fibroblasts Karpas 422 human lymphoma Kasumi-1 human Acute myeloid leukemia cells KB human Cervical carcinoma / epidermal carcinoma KB-3-1 human Cervical carcinoma / epidermal carcinoma KB-8-5 human Cervical carcinoma / epidermal carcinoma KB-V1 human Cervical carcinoma / epidermal carcinoma KBM-7 human Mononuclear cells (PMBC), T lymphocytes KC insect embryo KE-37 human KE-R cat embryo KELLY human neuroblastoma Kera-308 (308, Line 308) mouse skin Kera5 mouse keratinocyte KG-1 human Bone marrow (acute myeloid leukemia) KLN-205 mouse Squamous cell carcinoma KLU-2-R beef lung KM-H2 human lymphoma KM12 human colon cancer KMS-11 human myeloma cells KMS-27 human myeloma cells KNRK rat kidney KTR-R beef trachea Kym-1 human L-1236 human lymphoma L-41 human Acute myeloid leukemia cells L-428 human lymphoma L-540 human lymphoma L-929 mouse fibroblasts LM mouse fibroblasts LM (TK-) mouse fibroblasts L-WRN mouse subcutis L-cell line mouse fibroblasts L3.6pl human pancreatic cancer L6 rat Myoblast, musculature L660 human B-cell lymphoma L8057 mouse LAD2 human LAN 1 human neuroblastoma LAP1 mouse fibroblasts LC5 human human lung fibroblasts LC5-RIC human hepatocytes LCL-721 human Lymphocyte LCL-721174 human Lymphocyte LCL-WEI human B-cell LH86 human hepatoma LIM1215 human LinX human kidney LLC-MK2 original monkey kidney LLC-PK1 pig kidney LMH chicken hepatoma LN-229 human glioblastoma LN1590 human esophagus LNCAP human prostate cancer LNCaP C4-2 human prostate cancer LNCaP C4-2B human prostate cancer Loke human Merkel cell carcinoma LoVo human Adenocarcinoma (colon) LOX IMVI human melanoma LP9 / TERT-1 human peritoneal, mesothelial LR-7 mouse fibroblasts LS174T human Adenocarcinoma (colon) LUHMES human embryo, brain LUSIV human Hybrid between human B and T cell line LVIP2.0Zc mouse M-07e human acute megakaryocytic leukemia M-1 mouse kidney m-ICc12 mouse M14 human melanoma M7-Luc human Hybrid between human B and T cell line MA-104 monkey kidney MAC-1 human lymphoma MAGI human cervical cancer Makaka 2KM monkey lymphoblast Malme 3M human melanoma MaMel2 human melanoma MaMel35 human melanoma Mamur 3C monkey lymphoblast MaTi human Merkel cell carcinoma MaTu human mammary tumor MC-38 mouse Colon cancer MC-38cea mouse Colon cancer MCA 102 mouse fibrosarcoma McA-RH7777 rat hepatocytes MCC13 human Merkel cell carcinoma MCC26 human Merkel cell carcinoma McCoy mouse fibroblasts MCF-7 human Adenocarcinoma (breast) MCF-7 / ADR human breast carcinoma MCF10A human breast epithelium MCF10A ER-Src human breast epithelium MDA PCa 2b human prostate cancer MDA-MB-231 human Adenocarcinoma (breast) MDA-MB-435S human Epithelial cells, malignant melanoma, breast carcinoma (ductal) MDA-MB-453 human breast carcinoma MDA-MB-468 human Adenocarcinoma (breast) MDA-MET human Adenocarcinoma (breast) MDB1 human medulloblastoma MDBK beef kidney MDCK dog kidney MDCK.2 dog kidney Me-180 human cervical cancer MEB4 mouse melanoma MEC.B7.SigOVA (SAMBOK) mouse embryo MEC1 / 2 human B-cell lymphoma Mel 397 human melanoma Mel 526 human melanoma Mel 624 human melanoma Mel 888 human melanoma MESC 2.10 human embryo, brain MET-2 human epithelial cells MeT-5a human Bronchial mesothelial cells MeWo human Epithelial cells, malignant melanoma Mf4 / 4 mouse macrophages MG-63 human MG7 mouse MH-S mouse macrophages MIA PaCa-2 human pancreatic cancer mIMCD-3 mouse kidney Min 6 mouse pancreatic cells MJ [G11] human T-Lymphoblastomzelllinie MKL-1 human Merkel cell carcinoma MKN28 human Adenocarcinoma (stomach) MLE-12 mouse epithelial cells MLS402; MLS1765 human liposarcoma MLT-IFNAR - / - mouse liver MLT IRF3 - / - mouse liver MLT-MAVS - / - mouse liver MLT-WT mouse liver MLY-04 mouse osteocytes MM-HSE-2305 human testicle MM1.S human B-cell MM221-92; 221 monkey T cell MOLM-13 human Acute myeloid leukemia cells Molt-3 human T cell Molt-4 human T cell Molt-4/8 human T cell MonoMac6 human acute monocytic leukemia cells MOVAS mouse Aorta / smooth muscle MPC mouse pheochromocytoma mpf ferrets MRC-5 human human lung fibroblasts MS-LG seal lung epithelium MS1 mouse Pancreatic islet endothelium MS751 human cervical cancer MT-2 human lymphoblast MT-4 human lymphoblast MT 4puroFF human lymphoblast MT2 mouse Adenocarcinoma (breast) MTT mouse tumor Mv 1 Lu (NBL-7) ferrets lung epithelium MV3 human melanoma MV4-11 human Acute myeloid leukemia cells MYA 1 cat Mononuclear cells (PMBC), T lymphocytes MyDauDa bat intestine MyDauNi / 2c bat kidney N2A (Neuro-2a) mouse neuroblastoma N52 human amniocytes Namalwa human Burkitt's Lymphoma NB-1 human neuroblastoma Nb2 rat NB324K human kidney NB69 human neuroblastoma NCI-H1395 human lymphoblast NCI-H2170 human lung NCI-H226 human Squamous cell carcinoma (lung) NCI-H23 human Adenocarcinoma (lung) NCI-H292 human lung epithelium NCI H322M human human non-small cell lung carcinoma NCI-H460 human lung cancer NCI-H522 human Adenocarcinoma (lung) NCI-H727 human lung epithelium NCM460 human Colon Neuro-2A, N2A mouse neuroblasts NF - &#1008; B / 293 / GFP-Luc human kidney NF - &#1008; B / Jurkat / GFP human T cell NG108-15 Mouse / rat brain NIH3T3, NIH / 3T3, NIH-3T3 mouse fibroblasts NK-92 human natural killer cells, lymphoblast NMB human neuroblastoma NMU rat Adenocarcinoma (breast) nptr pig trachea NRK rat kidney NTERA-2 clone D1 human teratocarcinoma Nthy-ori 3-1 human epithelial cells OC 316 human Adenocarcinoma (ovary) OCI LY10 human B-cell lymphoma OCI LY4 human B-cell lymphoma OE19 human Oli-neu mouse oligodendrocytes OMK (637-69) monkey kidney OVCAR-3 human Adenocarcinoma (ovary) OVCAR-4 human Adenocarcinoma (ovary) OVCAR-5 human Adenocarcinoma (ovary) OVCAR-8 human Adenocarcinoma (ovary) P1-55 human Mononuclear cells (PMBC), T lymphocytes P3 / NS / 1-Ag4-1 mouse P388 mouse P3 HR-1 human B-cell P493-6 human B-cell lymphoma P815 mouse mastocytoma PA-1 human teratocarcinoma PA317 mouse fibroblasts Pac-1 human pancreatic cancer Panc 02 mouse pancreatic cancer Panc-1 human pancreatic cancer PAZ6 human Fatty tissue (brown) PC-12 rat pheochromocytoma PC-3 human adenocarcinoma PCL 12 human B-cell lymphoma PE501 mouse fibroblasts PEER human Pfeiffer human B-cell lymphoma PG-4 cat embryonic fibroblasts PG13 mouse fibroblasts PGP1 hiPSC human stem Cells PGP9 hiPSC human stem Cells Phoenix ampho / phi NX ampho human kidney Phoenix eco human kidney Phoenix-gp human kidney PIPNI bat kidney PIPNI / 1 bat kidney PK (15) pig kidney Platinum-A (Plat-A) human kidney Platinum E (Plat E) human kidney PLB-985 human Acute myeloid leukemia cells PLC / PRF / 5 human hepatocytes PM-1 human T cell PO lamb kidney psi 2 mouse fibroblasts psiAM mouse fibroblasts psiCRE mouse fibroblasts psiCRIP mouse fibroblasts PT1590 human esophagus PT67 mouse fibroblasts Pv11 insect embryo QM 7 quail QM 9 quail fibrosarcoma QT35 quail fibroblasts QT6 quail fibrosarcoma R 1610 hamster lung R06E flying Fox fibroblasts R1.1 mouse Raji human Burkitt's Lymphoma Ramos (RA1) human Burkitt's Lymphoma Ramos-EHRB human lymphoma Council1 rat fibroblasts Council2 rat fibroblasts RAW 264.7 mouse macrophages RBE-4 rat brain RBL-5 mouse lymphoma RC-37 monkey kidney RC2a human acute monocytic leukemia cells REF52 rat REF52 YFP-paxillin rat Rev-CEM human T cell RF / 6A monkey retina RH / 66A Rabbits T cell RH / K30 Rabbits T cell RH / K34 Rabbits Mononuclear cells (PMBC), T lymphocytes RHCT 138 Rabbits T-Lymphoblastomzelllinie RHEU-Lu bat lung RhiF-Lu bat lung RhiF-Mi bat RhiLu bat lung Rhini bat kidney RIKd bat kidney RIN 1046-38 rat Insulinom RINm5F rat Insulinom RK13 Rabbits kidney RL-5 Rabbits lymphoma RLE 6TN rat lung epithelium RMA mouse lymphoma RMA-S mouse lymphoma rMC rat kidney RoNi bat kidney RoNi ACE-2 bat kidney RoNi / 7 flying Fox kidney RPMI 8226 human B-cell RPTEC / TERT1 human kidney RS4; 11 human B-cell lymphoma RT-112 human bladder cancer RT4 human bladder cancer RTG 2 Rainbow trout fibroblasts RWPE-1 human epithelial cells RWPE-2 human epithelial cells S1A.TB.4.8.2 (replaces S1A.T4.G8) mouse S24 human glioblastoma S9 human human bronchial epithelial cells, (cystic fibrosis) SAOS-2 human osteosarcoma SAS human epithelial cells SAT human epithelial cells SB-HEK TRPM8 human kidney SC102A-1 human skin ScGT1 mouse neuroblasts Schneider-2 (S2) insect embryo SCL II human epithelial cells SCN immortalized mouse brain ScN2a mouse neuroblasts SCP-1 human stem Cells Seraphina human Burkitt's Lymphoma SF-21 insect ovaries SF-268 human glioblastoma SF-295 human glioblastoma SF-539 human gliosarcoma SF9 insect ovaries SGBS human Fatty tissue (brown) SH-SY5Y human neuroblastoma Shh light II mouse SiHa human cervical cancer SINCC mouse neuroblasts SIRC Rabbits SK-BR-3 human breast carcinoma SK-HEP-1 human Adenocarcinoma (liver) SK-MEL-2 human melanoma SK-MEL-28 human melanoma SK-MEL-3 human melanoma SK-MEL-5 human melanoma SK-N-AS human neuroblastoma SK-N-BE (2) human SK-OV-3 human Adenocarcinoma (ovary) SK6 pig kidney SKOV3.ip1 human ovarian cancer sMAGI monkey mammary tumor SMS-KAN human neuroblastoma SMS-KCN human neuroblastoma SMS-SB human lymphoma SN56 mouse neuroblastoma SNB-19 human glioblastoma SNB-75 human glioblastoma SNU-182 human hepatoma SNU-387 human hepatoma SP2 / 0-AG14 mouse myeloma cells ST8814 human SUM-1315 human breast carcinoma SUM-159 human breast carcinoma SUM149 human breast carcinoma SUP-T1 human T cell SV40 MES13 mouse kidney SVEC4-10 mouse endothelial SVG p12 human brain SW 982 human synovial sarcoma SW-48 human Adenocarcinoma (colon) SW1088 human brain SW13 human epithelial cells SW480 human colon cancer SW620 human Adenocarcinoma (colon) SW756 human cervical cancer SYO-1 human synovial sarcoma T-HESC human stromal T0281 human brain T1 human glioblastoma T24 human bladder cancer T269 human glioblastoma T325 human glioblastoma T3M-4 human pancreatic cancer T449, T778 human liposarcoma T47D human Breast carcinoma (ductal) T84 human Colon cancer T98G (T-98G) human glioblastoma TALL-1 human T cell TC-1 mouse lung epithelium TCAM 2 human testicle TE671 / RD human Rhabdomyosarcoma TF-1 human bone marrow TGP52 mouse Insulinom THLE-2, THLE-3 human liver THP-1 human Acute myeloid leukemia cells TIME human foreskin TMD5 human B-cell lymphoma TMD8 human B-cell lymphoma Toledo human B-cell lymphoma TRAMP C1-C3 mouse Prostate carcinoma TYK-nu human ovarian cancer TZM-bl human cervical cancer U-138 MG human glioblastoma U-2 OS human osteosarcoma U-251 human glioblastoma U-2932 human B-cell lymphoma U-373, U-373MG human glioblastoma U-87 MG human glioblastoma U-937 human lymphoma U1 human U38 human monocytes U3A human fibrosarcoma U937 human lymphoma UACC-257 human melanoma UACC-62 human melanoma UINS human Merkel cell carcinoma VISO-MEL-6 human melanoma UKE-1 human Acute myeloid leukemia cells UPCI: SCC090 human epithelial cells upcyte® endothelial cells human endothelial upcyte® hepatocytes human hepatocytes upcyte®LSEC human liver UPN 1 human lymphoma UT-SCC-14 human epithelial cells UT-SCC-5 human epithelial cells UT-SCC-8 human epithelial cells UWB1.289 + BRCA1 human ovarian cancer V79 hamster lung cell V79-4 hamster lung cell VCAP human Prostate carcinoma Vero monkey kidney Vero 76 monkey kidney Vero C1008 monkey kidney Vero LB-pi monkey kidney VERO B4 monkey kidney Vero MxA monkey kidney W12 human Cervical carcinoma / epidermal carcinoma WA01 (H1) human embryonic fibroblasts WA09 (H9) human embryonic fibroblasts WAGA human Merkel cell carcinoma WEHI-231 mouse B-cell lymphoma WI-38 human fibroblasts WIL2-S human B-cell WM-115 human Epithelial cells, malignant melanoma WPE-int human prostate WPE-stem human prostate WPE1-NB14 human epithelial cells WPE1-NB26 human epithelial cells WPMY-1 human Stromal cells from the prostate Wt MEF mouse embryonic fibroblasts WT100BIS human WT49 human WT51 human X63.Ag 8.653 mouse myeloma cells XC rat XF354 human epithelial cells XS106 mouse murine dendritic cell line XS52 mouse murine dendritic cell line Y79, Y-79 human retinoblastoma YT human lymphoma ZZ-R goat Fetus (tongue) ß-TC3 mouse Insulinom

Examples of important bacterial cultures and microbiological cultures are listed in Table 3. Table 3: Bacterial cultures and microbiological cultures Phylum "Acidobacteria" Class Acidobacteria Order Acidobacteriales Family Acidobacteriaceae Class Holophagae Order Acanthopleuribacterales Family Acanthopleuribacteraceae Order Holophagales Family Holophagaceae Phylum "Actinobacteria" Class Actinobacteria Subclass Acidimicrobidae Order Acidimicrobiales Suborder "Acidimicrobineae" Family Acidimicrobiaceae Family lamiaceae Subclass Actinobacteridae Order Actinomycetales Suborder Actinomycineae Family Actinomycetaceae Suborder Actinopolysporineae Family Actinopolysporaceae Suborder Catenulisporineae Family Actinospicaceae Family Catenulisporaceae Suborder Corynebacterineae Family Corynebacteriaceae Family Dietziaceae Family Gordoniaceae Family Mykobacteriaceae Family Nocardiaceae Family Segniliparaceae Family Tsukamurellaceae Family "Williamsiaceae" Suborder Frankineae Family Acidothermaceae Family Cryptosporangiaceae Family Frankiaceae Family Geodermatophilaceae Family Nakamurellaceae Family Sporichthyaceae Suborder Glycomycineae Family Glycomycetaceae Suborder Jiangellineae Family Jiangellaceae Suborder Kineosporiineae Family Kineosporiaceae Suborder Micrococcineae Family Beutenbergiaceae Family Bogoriellaceae Family Brevibacteriaceae Family Cellulomonadaceae Family Demequinaceae Family Dermabacteraceae Family Dermacoccaceae Family Dermatophilaceae Family Intrasporangiaceae Family Jonesiaceae Family Microbacteriaceae Family Micrococcaceae Family Promicromonosporaceae Family Rarobacteraceae Family Ruaniaceae Family Sanguibacteraceae Suborder Micromonosporineae Family Micromonosporaceae Suborder Propionibacterineae Family Nocardioidaceae Family Propionibacteriaceae Suborder Pseudonocardineae Family Pseudonocardiaceae Suborder Streptomycineae Family Streptomycetaceae Suborder Streptosporangineae Family Nocardiopsaceae Family Streptosporangiaceae Family Thermomonosporaceae Order Bifidobacteriales Family Bifidobacteriaceae Subclass Coriobacteridae Order Coriobacteriales Suborder "Coriobacterineae" Family Coriobacteriaceae Subclass Nitriliruptoridae Order Euzebyales Family Euzebyaceae Order nitrile iruptorales Family Nitriliruptoraceae Subclass Rubrobacteridae Alright Gaiellales Family Gaiellaceae Order Rubrobacterales Suborder "Rubrobacterineae" Family Rubrobacteraceae Order Solirubrobacterales Family Conexibacteraceae Family Patulibacteraceae Family Solirubrobacteraceae Order Thermoleophilales Family Thermoleophilaceae Phylum "Aquificae" Class Aquificae Order Aquificales Family Aquificaceae Family Desulfurobacteriaceae Family Hydrogenothermaceae Phylum "Armatimonadetes Class Armatimonadia Order Armatimonadales Family Armatimonadaceae Class Chthonomonadetes Order Chthonomonadales Family Chthonomonadaceae Class Fimbriimonadia Order Fimbriimonadales Family Fimbriimonadaceae Phylum "Bacteroidetes" Class Bacteroidia Order Bacteroidales Family Bacteroidaceae Family Marinilabiliaceae Family Porphyromonadaceae Family Prevotellaceae Family Prolixibacteraceae Family Rikenellaceae Class Cytophagia Order Cytophagales Family Catalimonadaceae Family Cyclobacteriaceae Family Cytophagaceae Family Flammeovirgaceae Family Mooreiaceae Family Rhodothermaceae Class Flavobacteriia Order Flavobacteriales Family Blattabacteriaceae Family Cryomorphaceae Family Flavobacteriaceae Family Schleiferiaceae Class Sphingobacteriia Order Sphingobacteriales Family Chitinophagaceae Family "Flexibacteraceae" Family Saprospiraceae Family Sphingobacteriaceae Phylum "Caldiserica" Class Caldisericia Order Caldisericiales Family Caldisericiaceae Phylum "Chlamydiae" Class Chlamydiae Order Chlamydiales Family Chlamydiaceae Family Parachlamydiaceae Family Simkaniaceae Family Waddliaceae Phylum "Chlorobi" Class Chlorobea Order Chlorobiales Family Chlorobiaceae Class Ignavibacteria Order Ignavibacteriales Family Ignavibacteriaceae Phylum "Chloroflexi" Class Anaerolineae Order Anaerolineales Family Anaerolineaceae Class Caldilineae Order Caldilineales Family Caldilineaceae Class Chloroflexi Order chloroflexales Suborder Chloroflexineae Family Chloroflexaceae Family Oscillochloridaceae Suborder Roseiflexineae Family Roseiflexaceae Order Herpetosiphonales Family Herpetosiphonaceae Class Dehalococcoidia Order Dehalococcoidales Family Dehalococcoidaceae Class Ktedonobacteria Order Ktedonobacterales Family Ktedonobacteraceae Family Thermosporotrichaceae Order Thermogemmatisporales Family Thermogemmatisporaceae Class Thermomicrobia Order Thermomicrobiales Family Thermomicrobiaceae Subclass Sphaerobacteridae Order Sphaerobacterales Suborder Sphaerobacterineae Family Sphaerobacteraceae Phylum "Chrysiogenetes" Class Chrysiogenetes Order Chrysiogenales Family Chrysiogenaceae Phylum "Cyanobacteria" Class Cyanobacteria Phylum "Deferribacteres" Class Deferribacteres Order Deferribacterales Family Deferribacteraceae Phylum "Deinococcus Thermus" Class Deinococci Okay Deinococcales Family Deinococcaceae Family Trueperaceae Order thermal Family Thermaceae Phylum "Dictyoglomi" Class Dictyoglomi Order Dictyoglomales Family Dictyoglomaceae Phylum "Elusimicrobia" Class Elusimicrobia Order Elusimicrobiales Family Elusimicrobiaceae Phylum "Fibrobacteres" Class Fibrobacteres Trim fibrobacteral Family Fibrobacteraceae Phylum "Firmicutes" Class Bacilli (alternatively: Firmibacteria) Order Bacillales Family Alicyclobacillaceae Family Bacillaceae Family Listeriaceae Family Paenibacillaceae Family Pasteuriaceae Family Planococcaceae Family Sporolactobacillaceae Family Staphylococcaceae Family Thermoactinomycetaceae Order Lactobacillales Family Aerococcaceae Family Carnobacteriaceae Family Enterococcaceae Family Lactobacillaceae Family Leuconostocaceae Family Streptococcaceae Class Clostridia Order Clostridiales Family Caldicoprobacteraceae Family Christensenellaceae Family Clostridiaceae Family Defluviitaleaceae Family Eubacteriaceae Family Gracilibacteraceae Family Heliobacteriaceae Family Lachnospiraceae Family Peptococcaceae Family Peptostreptococcaceae Family Ruminococcaceae Family Syntrophomonadaceae Order Halanaerobiales Family Halanaerobiaceae Family Halobacteroidaceae Order Natranaerobiales Family Natranaerobiaceae Order Thermoanaerobacterales Family Thermoanaerobacteraceae Family Thermodesulfobiaceae Class Erysipelotrichi Order Erysipelotrichales Family Erysipelotrichaceae Class negatives Order Selenomonadales Family Acidaminococcaceae Family Veillonellaceae Class Thermolithobacteria Order Thermolithobacteral Family Thermolithobacteraceae Phylum "Fusobacteria" Class fusobacteria Order Fusobacteriales Family Fusobacteriaceae Family Leptotrichiaceae Phylum "Gemmatimonadetes" Class Gemmatimonadetes Order Gemmatimonadales Family Gemmatimonadaceae Phylum "Lentisphaerae" Class Lentisphaeria Order Lentisphaerales Family Lentisphaeraceae Alright Victivallales Family Victivallaceae Class Oligosphaeria Order Oligosphaerales Family Oligosphaeraceae Phylum "Nitrospira Class "Nitrospira" Order "Nitro Spirals" Family "Nitrospiraceae" Phylum "Planctobacteria" Class Planctomycea (previously: Planctomycetacia) Order Planctomycetales Family Planctomycetaceae Class Phycisphaerae Order Phycisphaerales Family Phycisphaeraceae Phylum "Proteobacteria" Class Alphaproteobacteria Order Caulobacterales Family Caulobacteraceae Family Hyphomonadaceae Order Kiloniellales Family Kiloniellaceae Order Kordiimonadales Family "Kordiimonadaceae" Order Magnetococcales Family Magnetococcaceae Order "Parvularculales" Family "Parvularculaceae" Order Rhizobiales Family "Aurantimonadaceae" Family Bartonellaceae Family Beijerinckiaceae Family Bradyrhizobiaceae Family Brucellaceae Family Cohaesibacteraceae Family Hyphomicrobiaceae Family Methylobacteriaceae Family Methylocystaceae Family Phyllobacteriaceae Family Rhizobiaceae Family Rhodobiaceae Family Xanthobacteraceae Order Rhodobacterales Family Rhodobacteraceae Order Rhodospirillales Family Acetobacteraceae Family Rhodospirillaceae Order Rickettsiales Family Anaplasmataceae Family Holosporaceae Family Rickettsiaceae Okay sneathiellales Family Sneathiellaceae Order Sphingomonadales Family Erythrobacteraceae Family Sphingomonadaceae Class Betaproteobacteria Order Burkholderiales Family Alcaligenaceae Family Burkholderiaceae Family Comamonadaceae Family Oxalobacteraceae Family Sutterellaceae Order Hydrogenophilales Family Hydrogenophilaceae Order Methylophilales Family Methylophilaceae Alright Neisseriales Family Neisseriaceae Order Nitrosomonadales Family Gallionellaceae Family Nitrosomonadaceae Family Spirillaceae Order "Procabacteriales" Family "Procabacteriaceae" Order Rhodocyclales Family Rhodocyclaceae Class Gammaproteobacteria Order Acidithiobacillales Family Acidithiobacillaceae Family Thermithiobacillaceae Order Aeromonadales Family Aeromonadaceae Family Succinivibrionaceae Order Alteromonadales Family Alteromonadaceae Family Celerinatantimonadaceae Family Colwelliaceae Family Ferrimonidaceae Family Idiomarinaceae Family Moritellaceae Family Pseudoalteromonadaceae Family Psychromonadaceae Family Shewanellaceae Order Cardiobacteriales Family Cardiobacteriaceae Order Chromatiales Family Chromatiaceae Family Ectothiorhodospiraceae Family Granulosicoccaceae Family Halothiobacillaceae Family Thioalkalispiraceae Order "Enterobacteriales" Family Enterobacteriaceae Order Legionellales Family Coxiellaceae Family Legionellaceae Order Methylococcales Family Crenotrichaceae Family Methylococcaceae Order Oceanospirillales Family Alcanivoraceae Family Hahellaceae Family Halomonadaceae Family Litoricolaceae Family Oceanospirillaceae Family Oleiphilaceae Family "Saccharospirillaceae" Okay Orbales Family Orbaceae Order Pasteurellales Family Pasteurellaceae Order Pseudomonadales Family Moraxellaceae Family Pseudomonadaceae Order "Salinisphaerales" Family "Salinisphaeraceae" Order Thiotrichales Family Francisellaceae Family Piscirickettsiaceae Family Thiotrichaceae Order "Vibrional" Family Vibrionaceae Order Xanthomonadales Family Algiphilaceae Family Nevskiaceae Family Sinobacteraceae Family Solimonadaceae Family Xanthomonadaceae Class Deltaproteobacteria Order Bdellovibrionales Family Bacteriovoracaceae Family Bdellovibrionaceae Order Desulfarculales Family Desulfarculaceae Order Desulfobacterales Family Desulfobacteraceae Family Desulfobulbaceae Family Nitrospinaceae Order Desulfovibrionales Family Desulfohalobiaceae Family Desulfomicrobiaceae Family Desulfonatronumaceae Family Desulfovibrionaceae Order Desulfurellales Family Desulfurellaceae Order Desulfuromonadales Family Desulfuromonadaceae Family Geobacteraceae Order Myxococcales Suborder Cystobacterineae Family Cystobacteraceae Family Myxococcaceae Suborder Nannocystineae Family "Haliangiaceae" Family Kofleriaceae Family Nannocystaceae Suborder Sorangineae Family Phaselicystidaceae Family Polyangiaceae Family Sandaracinaceae Order Syntrophobacterales Family Syntrophobacteraceae Family Syntrophaceae Class Epsilonproteobacteria Order Campylobacterales Family Campylobacteraceae Family Helicobacteraceae Family "Hydrogenimonaceae" Order Nautiliales Family Nautiliaceae Class "Zetaproteobacteria Order "Mariprofundales" Family "Mariprofundaceae" Phylum "Spirochaetae" Class Spirochaetes Order Spirochaetales Family Brachyspiraceae Family Brevinemataceae Family Leptospiraceae Family Spirochaetaceae Phylum "Synergistic" Class Synergistia Order Synergistales Family Synergistaceae Phylum "Tenericutes" Class Mollicutes Order Acholeplasmatales Family Acholeplasmataceae Order anaeroplasmatales Family Anaeroplasmataceae Order entomoplasmatales Family entomoplasmataceae Family Spiroplasmataceae Order Haloplasmatales Family Haloplasmataceae Order mycoplasmaatales Family Mycoplasmaaceae Phylum "Thermodesulfobacteria" Class Thermodesulfobacteria Order Thermodesulfobacteriales Family Thermodesulfobacteriaceae Phylum "Thermotogae" Class thermotogae Order Thermotogales Family Thermotogaceae Phylum "Verrucomicrobia" Class Verrucomicrobiae Order Verrucomicrobiales Family Akkermansiaceae Family Rubritaleaceae Family Verrucomicrobiaceae Class Opitutae Order Opitutales Family Opitutaceae Order Puniceicoccales Family Puniceicoccaceae

Examples of important fungal cultures can be found in Table 4 Table 4: Fungus cultures Microsporidia inc. sed. Potty mushrooms (ChytridioMykota) NeocallimastigoMykota BlastocladioMykota ZoopagoMykotina KickxelloMykotina EntomophthoroMykotina (including fly-killers) MucoroMykotina GlomeroMykota (including arbuscular mycorrhizal fungi) Dikarya (ascomycetes and stamina mushrooms)

Examples of important viruses for viral cultures and diseases that you cause can be found in Table 5. Table 5: Overview of virus families, virus subfamilies, and viral genera Shrouded viruses ➢ Double-stranded DNA viruses = dsDNA • Family Poxviridae ◯ Subfamily Chordopoxvirinae ■ Genus Orthopoxvirus ■ Orthopoxvirus variola = Variola virus - smallpox, true smallpox ■ Orthopoxvirus variola var. Alastrim = kaffir pox virus - smallpox, white smallpox ■ Genus Parapoxvirus ■ Parapoxvirus ovis = Orf virus - Orf ■ Genus Molluscipoxvirus ■ Molluscum Contagiosum Virus - Molluscum contagiosum • Family Herpesviridae ◯ Subfamily Alphaherpesvirinae ■ Genus simplex virus ■ Herpes simplex virus 1 (HSV-1) = Human herpes virus 1 (HHV-1) - herpes simplex, herpes labialis, stomatitis aphtosa ■ Herpes simplex virus 2 (HSV-2) = Human herpes virus 2 (HHV-2) - herpes simplex, genital herpes ■ Herpes B virus = (herpesvirus simiae) ■ Genus Varicellovirus ■ Varicella-zoster virus (VZV) = human herpes virus 3 (HHV-3) - chickenpox = varicella (herpes zoster), shingles ■ suid herpesvirus type 1 (SHV-1) = pseudoprotective virus, Aujeszky's virus, etc. - Aujeszky's disease = pseudo-rash, itching, madcap, etc. (in animals, with low pathogenicity also transmissible to humans) ◯ Subfamily Betaherpesvirinae ■ Genus cytomegalovirus ■ Human cytomegalovirus (HCMV) = Human cytomegalovirus (HZMV) = Human herpes virus 5 (HHV-5) - cytomegalovirus ■ Genus Reseolovirus ■ Human herpesvirus 6 (HHV-6) - three-day fever ■ Human Herpesvirus 7 (HHV-7) - Three-Day Fever ◯ Subfamily Gammaherpesvirinae ■ Genus Lymphocryptovirus ■ Epstein-Barr virus (EBV) = Human herpes virus 4 (HHV-4) - Pfeiffer glandular fever, Burkitt's lymphoma ■ Genus Rhadinovirus ■ Human herpes virus 8 (HHV-8) - Kaposi's sarcoma • Family Hepadnaviridae ◯ multicellular genus Orthohepadnavirus ▪ Hepatitis B virus (HBV) - hepatitis B ➢ Single (+) strand RNA viruses = ss (+) RNA • Family Togaviridae ◯ Genus Alphavirus - pathogen of arboviruses ▪ Chikungunya virus (CHIKV) - Chikungunya fever ▪ Eastern equine encephalitis virus (EEEV) = Eastern equine encephalitis virus - mosquito transmission also possible on humans (rare!) → Encephalitis / encephalomyelitis [19] ▪ Western equine encephalitis virus (WEEV) = Western equine encephalitis virus - transmission by mosquitoes also possible on humans (rare!) → Encephalitis / encephalomyelitis [20] ▪ Everglades virus - Everglades fever ▪ O'Nongong-nyong virus (ONNV) - O'Nongong-nyong fever ▪ Mayaro fever virus Mayaro fever ▪ Semliki Forest Virus - Semliki Forest Fever ▪ Mucambo virus - Mucambo fever ▪ Ross River Virus - Ross River Fever ▪ Sindbis virus - Sindbis fever (joint inflammation ["epidemic polyarthritis"], partly with rashes and rarely with encephalitis) ◯ Genus Rubiviren ◯ Rubivirus = rubella virus = rubellavirus - rubella • Family Flaviviridae ◯ Genus Hepacivirus ▪ Hepatitis C virus (HCV) - hepatitis C ▪ GB virus C (without disease value) ◯ Genus Flavivirus ▪ West Nile Virus (WNV) - West Nile Fever ▪ Dengue virus (DENV) - Dengue fever ▪ Yellow fever virus (YFV) - yellow fever ▪ Louping ill virus (LIV) - Louping ill encephalitis ▪ St. Louis Encephalitis Virus (SLEV) - St. Louis Encephalitis ▪ Japan Encephalitis Virus (JEV) - Japanese Encephalitis ▪ Usutu virus (USUV) - nonspecific symptoms such as fever and / or rash ▪ Kyasanur Forest Disease Virus (KFDV) - Kyasanur Forest Fever ▪ Powassan virus (POWV) - Powassan encephalitis ▪ TBE virus [TBEV] - TBE (tick-borne encephalitis virus) - Subtype European / Western tick borne encephalitis virus (WTBEV) - Subtype Siberian tick-borne encephalitis virus (STBEV) - Subtype Far-Eastern tick borne encephalitis virus (Far Eastern TBEV); formerly Russian-Spring-Summer-Encephalitis-Virus (RSSEV) - RSSE, also RFSE (Russian-Spring-Summer-Encephalitis, Russian early-summer-encephalitis) ▪ Zika virus (ZIKV) (2 main groups, various subtypes) - usually only rash, fever, joint pain, conjunctivitis • Family Coronaviridae ◯ Subfamily Coronavirinae ◯ Genus Alphacoronavirus ▪ Human Coronavirus 229E (HCoV-229E) - Cold ◯ Genus Betacoronavirus ▪ Human Coronavirus OC43 (HCoV-OC43) - Cold ▪ SARS-associated coronavirus (SARS-CoV) - SARS (atypical pneumonia pneumonia). ▪ Middle East respiratory syndrome coronavirus (MERS-CoV) - flu-like symptoms, severe respiratory infection, pneumonia, and possibly renal failure ◯ Subfamily Torovirinae ◯ Genus Torovirus ▪ various types - gastroenteritis • Family Retroviridae - single (+) - strand RNA viruses with dsDNA intermediate ◯ Subfamily Orthoretrovirinae ◯ Genus deltaretrovirus ▪ Human T-lymphotropic Virus 1 (HTLV-1) - Adult T-Cell Leukemia, Tropical Spastic Paraparesis ▪ Human T-lymphotropic Virus 2 (HTLV-2) - Leukemia (?) ▪ Human T-lymphotropic virus 3 (HTLV-3) - unknown ▪ Human T-lymphotropic virus 4 (HTLV-4) - unknown ◯ Genus Lentivirus ▪ Human immunodeficiency virus type 1 (HIV-1) - AIDS ▪ Human immunodeficiency virus type 1 (HIV-2) - AIDS ➢ Single (-) - strand RNA viruses = ss (-) RNA • Family Arenaviridae ◯ Genus Arenavirus ◯ Chapare virus - Hemorrhagic fever ◯ Lassa virus - Lassa fever ◯ lymphocytic chorio-meningitis virus (LCMV) - lymphocytic choriomeningitis ◯ Lujo virus - Hemorrhagic fever ◯ Tacaribe virus - Hemorrhagic fever ◯ Junin virus - Junin fever (Argentinian haemorrhagic fever) ◯ Machupo virus - Machupo fever (Bolivian haemorrhagic fever) • Family Bornaviridae ◯ Genus Bornavirus ◯ Borna disease virus (BoDV) - Borna disease agent in horses and sheep, and possibly transmissible to humans - Affective disorders ◯ Mammalian bornavirus 1 ▪ Variegated squirrel Bornavirus 1 (VSBV-1) is detected in bunnies (Sciurus variegatoides), also transmissible to humans - potentially fatal encephalitis • Family Bunyaviridae - pathogens of Arboviruses ◯ Genus Orthobunyavirus ▪ Bunyamwera virus (serogroup) ▪ Batai virus (BATV) - flu-like symptoms and rashes ▪ California encephalitis virus (serogroup) - encephalitis ◯ Genus phlebovirus ◯ Rift Valley Fever Virus (3 subtypes) - Rift Valley Fever ◯ Sandfly fever virus (SFNV) - Sandfly fever = sandfly fever ▪ Subtype Karimabad virus (KARV) ▪ Subtype sandfly fever virus Sabin (SFNV-Sabin) ▪ Subtype Tehran virus (THEV) ▪ Subtype Toscana virus (TOSV) - Pappataci fever ▪ Serotypes: Tuscany (T), Sicily (S) and Naples (N) ◯ Genus Nairovirus ◯ Crimean Congo fever virus (serogroup): ▪ Subtype Crimean Congo Hemorrhagic Fever Virus (CCHFV) - Crimean Congo Fever ▪ Subtype Hazara virus (HAZV) - Crimean Congo fever ▪ Subtype Khasan virus (KHAV) - Crimean Congo fever ◯ Genus Hantavirus ◯ Hantaan virus (4 subtypes) - hemorrhagic fever, nephritis ◯ Seoul virus (serogroup) - hemorrhagic fever ◯ Prospect-Hill virus (2 subtypes) - hemorrhagic fever ◯ Puumala virus (serogroup) - haemorrhagic fever, pneumonia, nephritis ◯ Dobrava-Belgrade virus - haemorrhagic fever ◯ Tula virus - hemorrhagic fever ◯ Sin Nombre virus (serogroup) - haemorrhagic fever with severe pulmonary edema • Family Filoviridae ◯ Genus Marburg virus ◯ Lake Victoria Marburg virus (serogroup) - Marburg fever (haemorrhagic fever) ◯ Genus Ebola virus ◯ Zaire ebolavirus (ZEBOV) serogroup - Ebola fever (haemorrhagic fever) ◯ Sudan Ebola virus (SEBOV) serogroup - Ebola fever (haemorrhagic fever) ◯ Reston ebolavirus (REBOV) serogroup - not pathogenic to humans, haemorrhagic fever only in macaques and pigs ◯ Cote d'Ivoire ebolavirus (CIEBOV) serogroup - Ebola fever (haemorrhagic fever) ◯ Bundibugyo Ebola virus (BEBOV) serogroup - Ebola fever (haemorrhagic fever) • Family Orthomyxoviridae ◯ Genus influenza virus A - influenza (flu) ▪ Influenza virus A variant H1N1 - influenza (flu) ▪ Influenza virus A variant H3N2 - influenza (flu) ▪ (avian) influenza A variant H5N1, highly pathogenic avian influenza virus (HPAIV) - "avian influenza", in animals, also transmissible to humans, but hardly from person to person. ◯ Genus influenza virus B - influenza (flu) ▪ Influenza virus B / Victoria line - influenza (flu) ▪ Influenza virus B / Yamagata line - influenza (flu) ◯ Genus Influenza viruses C - Influenza (flu) • Family Paramyxoviridae ◯ Subfamily Paramyxovirinae ◯ Genus avulavirus ▪ Human parainfluenza virus (type 1, 3) - cold, parainfluenza ◯ Genus Morbillivirus ▪ Measles virus - measles ◯ Genus Henipavirus ▪ Hendra virus, (formerly Equines Morbillivirus) - pneumonia; encephalitis ▪ Nipah virus - pneumonia; encephalitis ◯ Genus Rubulaviruses ▪ Human parainfluenza virus (type 2, 4) - cold, parainfluenza ▪ Mumps virus - Mumps ◯ Subfamily Pneumovirinae ◯ Genus Pneumovirus ▪ Human respiratory syncytial virus (HRSV) (type A, B) - respiratory tract infection, common cold ◯ Genus metapneumovirus ▪ Human metapneumovirus (HMPV) (types A1 to 2, B1 to 2) - Respiratory tract infection, common cold • Family Rhabdoviridae ◯ Genus Vesiculovirus ▪ Vesicular stomatitis Indiana virus (VSV) - stomatitis vesicularis (oral mucosal inflammation with blistering) in animals, also transmissible to humans ◯ Genus Lyssavirus ▪ Rabies virus (RABV) (formerly genotype 1) = rabies virus - rabies, in animals, also transmissible to humans ▪ Mokola virus (MOKV) (formerly genotype 3) - rabies, in animals, also transmissible to humans ▪ Duvenhage virus (DUVV) (formerly genotype 4) - rabies, in animals, also transmissible to humans ▪ European bat Lyssa virus 1 + 2 (EBLV-1, -2) (formerly genotypes 5 and 6) - Rabies, in animals, also transmissible to humans ▪ Australian bat Lyssa virus (ABLV) (formerly genotype 7) - rabies, in animals, also transmissible to humans Uncovered viruses ➢ Double-stranded DNA viruses = dsDNA • Family Adenoviridae ◯ Genus mastadenovirus ▪ Human adenovirus AF (51 subtypes) - runny nose, colds, diarrhea • Family Polyomaviridae ◯ Genus Polyomavirus ▪ BK polyomavirus (BKPyV) = BK virus (BKV) = polyomavirus hominis type 1 - may lead to loss of the transplant during immunosuppressive treatment after transplantation ▪ JC Polyoma Virus (JCPyV) = JC virus (JCV) = polyomavirus hominis type 2 - in cellular immunosuppressed (AIDS) to progressive multifocal leukoencephalopathy (PML) • Family Papillomaviridae ◯ Genus Papillomavirus ▪ Subgenus human papillomaviruses ▪ various human papillomavirus (HPV) - warts ▪ Condyloma virus 6 (HPV-6) - genital warts ▪ Condyloma virus 11 (HPV-11) - genital warts ▪ Human papillomavirus 16/18/30 ... (HPV-16 / -18 / -30 ...) - cervical carcinoma = cervix tumor / cancer ➢ Single-stranded DNA viruses = ssDNA • Family Parvoviridae ◯ Subfamily Parvovirinae ▪ Genus Dependovirus ▪ Adeno-associated virus 2 (AAV-2) ▪ Adeno-associated virus 3 (AAV-3) ▪ Adeno-associated virus 5 (AAV-5) ▪ Genus erythroid virus ▪ Parvovirus B19 - Ring Rubella ➢ Double-stranded RNA viruses = dsRNA • Family Reoviridae ◯ Genus Rotavirus ▪ various types - gastroenteritis with diarrhea ◯ genus Coltivirus ▪ Colorado Tick Fever Virus - Colorado Tick Fever ➢ Single (+) strand RNA viruses = ss (+) RNA • Family Caliciviridae ◯ Genus Norovirus ▪ Norovirus (NV) = Norwalk-like virus (NLV) ▪ Humane noroviruses of groups GGI, GGII and GGIV - vomiting = gastroenteritis ◯ genus Sapovirus ▪ Sapovirus (SV) - Gastroenteritis • Family Hepeviridae ◯ Genus Hepevirus ▪ Hepatitis E virus (HEV) - hepatitis E • Family Picornaviridae ◯ Genus Enterovirus ▪ Poliovirus type 1-3 - polio ▪ Coxsackievirus A / B - from cold to meningitis, pancreatitis or myocarditis, rarely also paralyzes ▪ Coxsackievirus B1 (CVB-1) to B 6 - Cold ▪ Echovirus Exanthema Enanthema, upper respiratory tract infections (colds), herpangina, myopericarditis, disseminated neonatal infection, chronic meningoencephalitis in immunosuppressed patients, meningitis, encephalitis rare Paralysis ▪ enterovirus ▪ Human Enteroviruses - Cold ▪ Human enterovirus 70 (EV-70) - acute hemorrhagic conjunctivitis ▪ Human enterovirus 71 (EV-71) - meningoencephalitis, skin rash, and poliomyelitis-like syndrome = hand-foot-and-mouth disease ◯ genus Hepatovirus ▪ Hepatitis A virus - hepatitis A ◯ genus rhinovirus ▪ rhinovirus ▪ Human Rhinovirus-1 A (HRV-1 A) or 1 B to 100 - Cold oncoviruses The group of "oncoviruses", the most important in humans carcinogenic (carcinogenic) viruses, is responsible for 10 to 15 percent of all human cancers worldwide, even accounting for about 17% of cancer cases in the US Cancer Society. ^{[22] [23]} • Epstein Barr virus (EBV) • Hepatitis B virus (HBV) • Hepatitis C virus (HCV) • human papillomavirus (HPV) • Human T-lymphotropic virus 1 (HTLV-1) • Human herpesvirus 8 (HHV-8, also Kaposi's sarcoma herpesvirus, KSHV) Giant viruses • Nucleocytoplasmic large DNA viruses

The enormous number of serious diseases causing mycoplasmas, viruses and other microorganisms emphasize the importance of pure, mycoplasmic eukaryotic cultures, viral cultures and cultures of microorganism populations in controlling and researching diseases.

Laboratories for biochemistry, molecular biology, genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology and medicine, and items, clothing, equipment and apparatus for and in these laboratories

• - der Prophylaxe und/oder der Nachbehandlung von Mollicuteninfektionen und/oder Mollicutenkontaminationen, insbesondere Mykoplasmeninfektionen und Mykoplasmenkontaminationen,
• - der temporären oder dauerhaften Abtötung und/oder Dekontaminierung von Mollicutenkontaminationen, insbesondere Mykoplasmenkontaminationen
• - des temporären oder dauerhaften Abstoppens und/oder der temporären oder dauerhaften Inhibierung der Vermehrung von Mollicuten, insbesondere Mykoplasmen, in Kulturen und/oder
• - der temporären oder dauerhaften Einstellung einer konstanten Konzentration von Mollicuten, insbesondere Mykoplasmen,

mit den vorstehend beschriebenen POM und/oder den POM-Präparationen vermischt, behandelt, gereinigt und/oder beschichtet werden. Dabei können diese separat in verschiedenen Reihenfolgen und als

• - in Form einer Säure, eines Salzes, eines Hydrats, eines Soft-Oxometallats (SOMS), einer Beschichtung und/oder eines Polyoxometallat-Nanocomposits, und/oder
• - in molekulardispers gelöster, gepufferter, suspendierter, lipophilisierter und/oder mit organischen Gruppen fluidisierter Form und/oder
• - in und/oder auf als sich dispergierenden, teilweise auflösenden, nicht auflösenden oder völlig auflösenden, kompakten und/oder porösen Partikeln, Beschichtungen, Coatings, Releasematerialien, Schwämmchen, Plättchen, Folienstückchen, Textilstückchen, Gewebestückchen, Cellulosefasern, Cellulosemikropartikeln, Cellulosenanopartikeln, Nanosomen, Mikrosomen, Liposomen, Tabletten, Glaskügelchen, Sprühgeräten, Verneblern und/oder als Beschichtung, die die Oberfläche hart und glatt und/oder diffusionsgeschlossen macht, und/oder
• - in Aerosolen, Nebeln, Hydrogelen, in Micellen, in Vesikeln, in Mikrokapseln, in Mikrosphären, in Gelen, in Cremes, Lotions, Salben und/oder Schäumen und/oder
• - in Verbindung mit funktionalen Materialien, Antibiotika, bioziden Materialien und/oder Nährmedien und/oder Hilfsmedien für die Zellbiologie und/oder
• - in Form seiner Ausgangsprodukte, die sich spontan zu dem mindestens einen Polyoxometallat zusammenfügen,

eingebracht werden.The following are exemplary and not exhaustive enumerated laboratories for biochemistry, molecular biology, genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology and medicine, as well as the items, clothing, implements and equipment for and in these laboratories for purposes

• the prophylaxis and / or after-treatment of mollicut infections and / or mollicut contamination, in particular mycoplasma infections and mycoplasma contamination,
• the temporary or permanent killing and / or decontamination of mollicut contaminants, in particular mycoplasma contamination
• the temporary or permanent stopping and / or the temporary or permanent inhibition of the proliferation of mollicutes, in particular mycoplasmas, in crops and / or
• the temporary or permanent setting of a constant concentration of mollicutes, especially mycoplasmas,

are mixed, treated, purified and / or coated with the POM and / or POM preparations described above. These can be separated in different orders and as

• in the form of an acid, a salt, a hydrate, a soft oxometalate (SOMS), a coating and / or a polyoxometalate nanocomposite, and / or
• - in molecularly dispersed, buffered, suspended, lipophilized and / or fluidized with organic groups form and / or
• - in and / or as dispersing, partially dissolving, non-dissolving or completely dissolving, compact and / or porous particles, coatings, coatings, release materials, sponges, platelets, foil pieces, textile pieces, fabric pieces, cellulose fibers, cellulose microparticles, cellulosic nanoparticles, nanosomes, microsomes , Liposomes, tablets, glass beads, sprayers, nebulisers and / or as a coating that renders the surface hard and smooth and / or diffusion-closed, and / or
• in aerosols, mists, hydrogels, micelles, vesicles, microcapsules, microspheres, gels, creams, lotions, ointments and / or foams and / or
• - in conjunction with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary media for cell biology and / or
• in the form of its starting materials, which spontaneously combine to form the at least one polyoxometalate,

be introduced.

Hereinafter, items, clothing, utensils, furniture, prints and apparatuses conventionally used in the aforementioned laboratories are enumerated.

According to the use according to the invention, and the POM and / or the POM preparations are added in a dosage that set the Mollicuten killing and / or inhibiting limit concentrations.

According to the sterilization method according to the invention, the POM and / or the POM preparations are added in dosages such that concentrations> adjust the limit concentrations.

• o Deckel von Flaschen die in der Zellkultur verwandet werden, auch Spritz- und Sprühflaschen
• o In einem separaten Dichtungsring oder Inlet in Flaschen oder Kulturgefaessen
• o im 3D-Printmaterial
• o Flaschen Innenbeschichtung und Aussenbeschichtung
• o Eine Sprühloesung zur Desinfektion
• o Eine Sprühloesung, Suspension, etc. in Kombination mit Ethanol, 95% und jede andere Konzentration
• o Laborglas, Gefäße
• o Becher
• o Erlenmeyerkolber
• o Messbecher
• o Abdichtungen
• o Inlets für Abdichtungen
• o Mischzylinder
• o Messkolben
• o Stopfen aus Kunststoff und Glashohlstopfen
• o Pyknometer
• o Sedimentiergefäße o Glasflaschen und Kunststoffflaschen
• o Abklärflaschen
• o Tropfflaschen
• o Gewindeflaschen
• o Ausgiessringe
• o Oliven
• o Septen
• o Flaschenmehrfachverteiler
• o Rührreaktoren
• o Exsikkatoren
• o Probenflaeschchen
• o Autosamplervials
• o Analysen und Szintillationsgefäße
• o Rührstaebe
• o Wischer
• o Probenroehrchen
• o Reagenzglaeser
• o Stopfen
• o Zentrifugenroeehrchen
• o Zentrifugenflaschen
• o Sicherheitsflaschen
• o Fässer
• o Kanister
• ○ Ballonflaschen
• ○ Spreuhflaschen
• ○ Dosen
• ○ Proben und Versandgefaesse
• ○ Laborschalen und Wannen
• ○ Abtropfbretter
• ○ Instrumentenschalen
• ○ Tabletts
• ○ Schüsseln
• ○ Nierenschalen
• ○ Eimer
• ○ Klemmen
• ○ Übergangsstücke
• ○ Destillationssysteme
• ○ Chromatographiesaeulren
• ○ Scheidetrichter
• ○ Trichter
• ○ Nutschen
• ○ Gasflaschen
• ○ Wulfscheflaschen
• ○ Gaswaschflaschen
• ○ Probenschalen
• ○ Brutschränke
• ○ Kristallisierschalen
• ○ Uhrgläser
• ○ Mörser und Reibschalen
• ○ Abdampfschalen
• ○ Laborporzellan
• ○ Wägezubehöhr
• ○ Siebe
• ○ Siebkellen
• ○ Löffel
• ○ Schaufeln
• ○ Spatel
• ○ Hebebühnen
• ○ Stativmaterilaien
• o Zangen
• o Clips
• o Halter
• o Schwimmständer
• o Staender fuer Vials und Probenroehrchen
• o Reagenzglasgestelle
• o PCR Staender
• o Cryoboxen und Gestelle
• o Dewars
• o Wasserbad
• o Ultraschallbad
• o Folien
• o Spatel
• o Parafilm
• o Sterilisationsinstrumente wie Körbe nd Container und Sterilisatoren
• o Zellschaber
• o Rollerflaschen
• o Kulturflaschen
• o Petrischalen
• o Kolonienzählgeraete
• o Standlupe und Leuchtplatte
• o Rotavaporen
• o Kontaktschalen
• o Petrieschalen mit Gitternetze, Teilungen
• o Zahnstocher
• o Imfpösen
• o Impfnadeln
• o Wattestäbchen
• o Klontransferdiscs
• o Homogenisatoren
• o Mikropistille
• o Küvetten
• o Abdeckungen für Küvetten
• o Adhäsionsobjekttraeger
• o Färbeplatten
• o Objekttraegerspender
• o Versandbehälter auch für Objektträger
• o Präparatenmappen
• o Färbkammern
• o Einbettkasetten
• o Pinzetten
• o Skalpelle und Klingen
• o Scheren
• o Klemmen und Nadeln
• o Zangen
• o NAHMTaterial
• o Präpariernadeln und Sonden
• o Präparationszubehör
• o Nutschen
• o Autoklaven
• o Blockthermostate
• o Brenner und Anzünder
• o Brutschränke
• o Dampfsterisisatoren
• o Dispergier und Homogenisiersysteme
• o Drei- und Vierfüsse und Hitzplatten
• o Eisautomaten
• o Heizer, Magnetruehrer
• o Gefrierschraenke
• o Heissluftgebläse
• o Heissluftsterilisatoren
• o Heiz- und Magnetrührer
• o Heizgeräte und Laborkocher
• o Heizhauben und Heizleitungen
• o Histologie und Pathologiegeräte
• o Inkubationsschüttler
• o Kältethermostate
• o Heiswasserbereiter
• o Kontaktthermometer
• o Kühlbrutschränke
• o Gefriertruhen und -schränke
• o Begehbare Gefrier- und Kühlsysteme
• o Magnetstäbchen
• o Mikrowellengeräte
• o Mühlen und Mixer
• o Muffelofen
• o Ölbäder
• o Rocker
• o Rotatoren
• o Tauchsieder
• o Thermoshaker
• o Thermostatschränke
• o Überkopfmischer
• o Ultraschallsysteme wie Lanzen und Desintegratoren
• o Umlaufkühler
• o Vakuuum-Trockenschränke
• o Wasserbäder
• o Laborbedarf fuer Probennahme, Pumpen und Liquid Handling
• o Druckminderer
• o Probennehmer
• o Pumpen, wie Membranpumpen
• o Peristaltische Pumpen
• o Membranvakuumpumpen
• o Diffusionsvakuumpumpen
• o Dampfstrahlvakuumpumpen
• o Drehschieberpumpen
• o Vakuummessgeräte
• o Absaugsysteme
• o Klimaanlagen
• o Schleusen
• o Wasserstrahlpumpe
• o Schläuche
• o Schlachklemmen und Binder und Verbinder
• o Hähne
• o Dispenser
• o Büretten
• o Spritzen und Kanuelen
• o Pasteurpipetten und Gummisauger
• o Einmalpipetten
• o Voll-/Messpipetten
• o Pipettierbälle
• o Pipettierhilfen auch durch Akkus und elektrisch betrieben
• o Pipettenreinigungsbehälter
• o Dispenserpipetten
• o Dispensertips
• o Direktverdraengerpipetten
• o Mikroliterpipetten
• o Pipettenspitzen
• o Pipettenspitzennachfüllsystem
• o Sicherheitsreaktionsgefäße
• o Etiketten und Lab-Marker

o Laborbedarf für Filtration, Wasseraufbereitung und Dialyse

• o Spritzenfilter
• o Filtereinheiten
• o Zentrifugenfiltereinheiten
• o Bottle-top-Filtersysteme
• o Filterhalter
• o Membranfilter
• o Filterpapiere
• o Extraktionshülsen
• o Analysensiebe
• o Wasseraufbereitungsgeräte
• o lonenautstauschepatronen
• o Wasseraufbereitung
• o Kapillarröhrchen
• o Zerstäuber
• o Vakuumblöcke
• o SPE-Säulen
• o Dialyseschlauchmembranen
• o Dialysesysteme und Zubehör

o Laborbedarf für Arbeitsschutz und Sicherheit

• o Körperduschen
• o Augenduschen und Wasseramatueren
• o Augenspülflaschen
• o Brillen
• o Kopf- und Gesichtsschutz
• o Mundschutz
• o Atemschutz
• o Gehörschutz
• o Hautpflege
• o Hautreinigung
• o Hautdesinfektion
• o Handschuhe
• o Berufskleidung wie Kittel, Hauben, Überschuhe
• o Erstehilfekasten
• o Verbandsmaterial
• o Schutzschilder
• o Kennzeichnungen
• o Entsorgungseimer und Behaelter

o Laborausstattung

• o Anti-Rutschmatten
• o Aufbewahrungsbehälter
• o Büroorganisationssysteme
• o Glasschneider und Graviergeräte
• o Kisten und Boxen
• o Klemmen
• o Koffer
• o Leitern
• o Messer
• o Öffner
• o Plattformwagen
• o Scheren
• o Schränke
• o Labottische
• o Abzüge
• o Stehhilfen
• o Transportwagen
• o Werkzeuge

o Various equipment and appliances

• o Lid of bottles used in cell culture, also spray and spray bottles
• o In a separate sealing ring or inlet in bottles or culture vessels
• o in 3D print material
• o Bottles inside and outside
• o A spray solution for disinfection
• o A spray solution, suspension, etc. in combination with ethanol, 95% and any other concentration
• o laboratory glass, vessels
• o mug
• o Erlenmeyer Colbert
• o Measuring cup
• o seals
• o Inlets for seals
• o mixing cylinder
• o volumetric flask
• o plastic plugs and glass stopper
• o pycnometer
• o sedimentation vessels o glass bottles and plastic bottles
• o suds
• o dropper bottles
• o Threaded bottles
• o pouring rings
• o olives
• o septa
• o Bottle dispenser
• o stirred reactors
• o Desiccators
• o Sample bottle
• o Autosamplervials
• o Analyzes and scintillation vials
• o Rührstaebe
• o wiper
• o Sample tube
• o Test tube
• o stopper
• o Centrifuge tubes
• o Centrifuge bottles
• o Safety bottles
• o barrels
• o canister
• ○ Balloon bottles
• ○ Spreader bottles
• ○ canned
• ○ Samples and shipping containers
• ○ laboratory trays and tubs
• ○ Draining Boards
• ○ Instrument trays
• ○ Trays
• ○ Bowls
• ○ kidney dishes
• ○ bucket
• ○ terminals
• ○ Transition pieces
• ○ Distillation systems
• ○ Chromatography
• ○ Separating funnels
• ○ Funnel
• ○ Nutsche
• ○ Gas bottles
• ○ Wulfscheflaschen
• ○ Gas washing bottles
• ○ Sample cups
• ○ incubators
• ○ crystallizing dishes
• ○ watch glasses
• ○ Mortar and friction bowls
• ○ Evaporating dishes
• ○ laboratory porcelain
• ○ Weighing accessories
• ○ sieves
• ○ sieve trowels
• ○ Spoon
• ○ Shovels
• ○ spatula
• ○ Lifting platforms
• ○ Tripod materials
• o pliers
• o clips
• o holder
• o Floating stand
• o Stands for vials and sample tubes
• o Test tube racks
• o PCR stators
• o Cryoboxes and racks
• o Dewars
• o water bath
• o ultrasonic bath
• o slides
• o spatula
• o Parafilm
• o Sterilization instruments such as baskets, containers and sterilizers
• o Cell scraper
• o Roller bottles
• o Culture bottles
• o Petri dishes
• o Colonial Counters
• o Stand magnifier and light panel
• o Rotavaporen
• o contact cups
• o Petri dishes with lattices, divisions
• o toothpick
• o Imfpösen
• o vaccine needles
• o Cotton swabs
• o clone transfer discs
• o Homogenizers
• o Micropistilla
• o cuvettes
• o Covers for cuvettes
• o Adhesion object carrier
• o dyeing plates
• o Object carrier dispenser
• o Shipping containers also for slides
• o preparation files
• o dyeing chambers
• o Embedded cassettes
• o tweezers
• o scalpels and blades
• o scissors
• o clamps and needles
• o pliers
• o Raw material
• o Dissecting needles and probes
• o Preparation accessories
• o Nutsche
• o autoclave
• o Block thermostats
• o burner and lighter
• o incubators
• o steam sterilizers
• o Dispersing and homogenizing systems
• o three and four feet and hot plates
• o ice machines
• o heater, magnetic driver
• o Freezer
• o Hot air blower
• o Hot air sterilizers
• o Heating and magnetic stirrer
• o heaters and laboratory cookers
• o Heating mantles and heating cables
• o histology and pathology devices
• o Incubation shaker
• o cooling thermostats
• o Hot water heater
• o contact thermometer
• o refrigerated incubators
• o Freezers and cupboards
• o Walk-in freezing and cooling systems
• o Magnetic sticks
• o microwave ovens
• o mills and blenders
• o muffle furnace
• o oil baths
• o Rocker
• o rotators
• o immersion heaters
• o Thermoshaker
• o Thermostatic cabinets
• o overhead mixer
• o Ultrasound systems such as lances and disintegrators
• o circulation cooler
• o Vacuum drying cabinets
• o water baths
• o Laboratory equipment for sampling, pumps and liquid handling
• o Pressure reducer
• o sampler
• o Pumps, such as diaphragm pumps
• o Peristaltic pumps
• o Diaphragm vacuum pumps
• o diffusion vacuum pumps
• o steam jet vacuum pumps
• o Rotary vane pumps
• o Vacuum gauges
• o Suction systems
• o Air conditioners
• o locks
• o Water jet pump
• o hoses
• o Schlachklemmen and binders and connectors
• o taps
• o Dispensers
• o Burettes
• o spraying and canoeing
• o Pasteur pipettes and rubber suction cups
• o disposable pipettes
• o Full / graduated pipettes
• o Pipetting balls
• o Pipetting aids also by batteries and electrically operated
• o Pipette cleaning container
• o Dispenser pipettes
• o Dispenser tips
• o direct-displacement pipettes
• o microliter pipettes
• o pipette tips
• o Pipette tip refilling system
• o Safety reaction vessels
• o Labels and lab markers

o Laboratory equipment for filtration, water treatment and dialysis

• o Syringe filter
• o Filter units
• o Centrifuge filter units
• o Bottle-top filter systems
• o Filter holder
• o membrane filter
• o Filter papers
• o extraction sleeves
• o Test sieves
• o Water treatment equipment
• o lonenautstauschepatronen
• o water treatment
• o capillary tube
• o atomizer
• o Vacuum blocks
• o SPE columns
• o dialysis tubing membranes
• o dialysis systems and accessories

Laboratory requirements for occupational safety and security

• o body showers
• o Eye showers and water amaturs
• o eye wash bottles
• o glasses
• o head and face protection
• o Mouthguard
• o Respiratory protection
• o Hearing protection
• o skin care
• o skin cleansing
• o skin disinfection
• o gloves
• o Work clothes such as gowns, hoods, overshoes
• o First aid box
• o dressing material
• o Protection labels
• o Markings
• o Disposal buckets and containers

o Laboratory equipment

• o Anti-slip mats
• o storage box
• o Office organization systems
• o Glass cutters and engravers
• o boxes and boxes
• o clamps
• o suitcases
• o ladders
• o knife
• o opener
• o platform car
• o scissors
• o Cabinets
• o Labotti
• o Deductions
• o Standing aids
• o Dolly
• o tools

• o Batterien und Ladegeräte
• o Infrarotleuchten
• o Kaltlichtquellen
• o Laserpointer
• o Leuchten
• o Leuchtlupen
• o Leuchtplatten
• o Linsenreinigungsmittel
• o Mikrospkope
• o Refraktometer
• o Röntgengeraete und Kasetten
• o Steromikroskope
• o Transilluminatoren
• o UV-Leuchten, germizid und nicht germizid
• o Bildschirme
• o Computer, EDV, mobile und stationäre Telefonie
• o Beamer

Laboratory requirements for optical and electronic instruments and luminaires

• o batteries and chargers
• o infrared lights
• o cold light sources
• o laser pointer
• o lights
• o Light magnifying glasses
• o Light panels
• o lens cleaner
• o microspheres
• o Refractometer
• o X-ray machines and cassettes
• o Steromicroscopes
• o Transilluminators
• o UV lights, germicidal and non-germicidal
• o screens
• o Computer, EDP, mobile and stationary telephony
• o Beamer

• o Barometer
• o Datenlogger
• o Dichtemessgeräte
• o Thermometer
• o Gasmessgeräte
• o Gasflowkontroller
• o pH-Messgeräte
• o Leitfähigkeitsmesser
• o Luxmeter
• o Photometer
• o pH-Papiere
• o pH-Pufferlösungen
• o Sauerstoffmessgeräte
• o Taschenrechner
• o Testpapiere
• o Thermohygrometer
• o Trübungsmessgeräte
• o Uhren
• o Analysen- und Präzisionswaagen
• o Bücher, Tabellen
• o Schallmessgeräte
• o Heliumlecksuchgeräte für Hochvakuumapparaturen

o Laborbedarf an Wandtafeln

• o Wandtafeln mit Beamer verbunden

o Laborbedarf für Reinigung und Pflege

• o Fette und Wachse
• o Schutzpapiere wie Bench-Top
• o Wischer
• o Büroreiniger
• o Bürsten, Reinigungsutensilien
• o Desinfektionsmittel
• o Handgels und Körpergels sowie Sprühmittel
• o Kombination mit Ethanol, wie 95%-igem Ethanol
• o Isopropanol
• o Entkalker
• o Etikettenlöser
• o Gaslecksucher
• o Glasperen und Beads
• o Giesharze, Epoxide
• o Heizbadflüssigkeiten
• o Instrumentendesinfektionsmittel
• o Klebe- und Isolierbänder
• o Klebstoffe
• o Konservierer
• o Lösemittel
• o Magnesiastäbchen und Rinnen
• o Laborspülmittel
• o Laborspülmaschinen
• o Schmiermittel
• o Siedesteinchen
• o Sauger
• o Sprays
• o Trennmittel
• o Wasserbadkonservierer
• o Wischtücher

◯ Hilfsmittel für die Elektrophorese

• ◯ PAGE-Gele (Polyacrylamid-Gelelektrophorese)
• ◯ PAGE-Lösungen
• ◯ PAGE-Reagenzien
• ◯ Agarosen und Gelbildner
• ◯ Ampholyte
• ◯ Gelpufferlösungen
• ◯ Gelladepuffer
• ◯ Marker und Standards
• ◯ Gelfärbung
• ◯ Kammern
• ◯ Blotting

◯ Hilfsmittel für die Molekularbiologie

• ◯ DNA und RNA Isolation
• ◯ DANN und RNA analyse
• ◯ PCR

◯ Hilfsmittel für die Biochemie

• ◯ Biologische Puffersalze
• ◯ Detergenzien
• ◯ Enzyme
• ◯ Coenzyme
• ◯ Inhibitoren
• ◯ Substrate
• ◯ Albumine
• ◯ Proteinisolierung
• ◯ Szintillation
• ◯ Proteinquantifizierung
• ◯ Western-Blot und ELISA (Enzyme-linked Immunosorbent Assay)

◯ Hilfsmittel fürdie Mikrobiologie

• ◯ Hygienekontrolle
• ◯ Fertignährmedien
• ◯ Trockennährmedien
• ◯ Nährmedienzusätze
• ◯ Nährmediengrundstoffe
• ◯ Geliermittel
• ◯ Antibiotika und Antimykotika
• ◯ Cryokonservierung

◯ Hilfsmittel für die Histologie und die Mikroskopie

• ◯ Intermedien
• ◯ Reagenzien
• ◯ Fixierung
• ◯ Einbettung
• ◯ Färbung
• ◯ Einschlussmittel
• ◯ Zubehöhr für Mikroskopie

◯ Hilfsmittel für die Zellbiologie

• ◯ Hygienekontrolle
• ◯ Fertignährmedien
• ◯ Trockennährmedien
• ◯ Nahermedienzusaetze
• ◯ Pinzetten
• ◯ Petrieschalen
• ◯ Aufbewahrungsgefäße
  • ■ Zellkulturflachen
  • ■ Zellkulturflaschen mit Filter-Schraubverschluss, z.B. transparent
  • ■ Filter in den Zellkulturflaschen
  • ■ Suspensionskulturflaschen, aus z.B. PS, z.B. transparent
  • ■ Zellkultur-Multiwellplatten
  • ■ Mit Oberflächenbehandlungen wie DNase, RNase und/oder Human-DNA-frei
• ◯ Adhäsionsobjektträger
• ◯ Handschuhe, Nitrilix und Latex, gepudert und ungepudert
• ◯ Unterhandschuhe
• ◯ Einweg- und Mehrweghandschuhe
• ◯ Gesichtsmasken und Mundschutz, einweg und mehrweg, mit Filter und ohne Filter
• ◯ Filter der Mundmasken
• ◯ Arbeitskleidung, insbesondere Laborkittel, Laborhose, Laborschuhe
• ◯ Laborhaarbedeckung und Netze
• ◯ Ganzkörperanzüge und Schutzanzüge
• ◯ Überschuhe und Schuhschutze
• ◯ Knöpfe für Laborkleidung
• ◯ Seal für die Mikroskopie
• ◯ Mikroskopierträger

Laboratory requirements for measuring instruments

• o Barometer
• o Datalogger
• o Density meters
• o thermometer
• Gas detectors
• o Gas flow controller
• o pH meters
• o conductivity meter
• o Luxmeter
• o photometer
• o pH papers
• o pH buffer solutions
• o oxygen meters
• o Calculator
• o test papers
• o Thermohygrometer
• o Turbidity meters
• o clocks
• o Analytical and precision balances
• o books, tables
• o Sound meters
• o Helium leak detectors for high vacuum equipment

o Laboratory equipment on wall panels

• o Wall panels connected to beamer

o Laboratory equipment for cleaning and care

• o fats and waxes
• o protective papers such as bench top
• o wiper
• o Office cleaner
• o brushes, cleaning utensils
• o disinfectant
• o Handgels and body gels as well as sprays
• o Combination with ethanol, such as 95% ethanol
• o isopropanol
• o descaler
• o Label remover
• o Gas leak detector
• o glass beads and beads
• o Giesharze, epoxides
• o heating bath liquids
• o Instrument disinfectant
• o adhesive and insulating tapes
• o adhesives
• o Conservator
• o solvent
• o Magnesia sticks and gutters
• o Laboratory detergent
• o Laboratory dishwashers
• o lubricant
• o boiling stones
• o sucker
• o sprays
• o release agent
• o Waterbath preservative
• o wipes

◯ Aid for electrophoresis

• ◯ PAGE gels (polyacrylamide gel electrophoresis)
• ◯ PAGE solutions
• ◯ PAGE reagents
• ◯ Agaroses and gelling agents
• ◯ ampholytes
• ◯ gel buffer solutions
• ◯ gel loading buffer
• ◯ markers and standards
• ◯ gel staining
• ◯ chambers
• ◯ blotting

◯ Auxiliary for molecular biology

• ◯ DNA and RNA isolation
• THEN and RNA analysis
• ◯ PCR

◯ Auxiliary for biochemistry

• ◯ biological buffer salts
• ◯ Detergents
• ◯ enzymes
• ◯ coenzymes
• ◯ inhibitors
• ◯ Substrates
• ◯ albumins
• ◯ protein isolation
• ◯ Scintillation
• ◯ protein quantification
• ◯ Western Blot and ELISA (Enzyme-linked Immunosorbent Assay)

◯ Auxiliary for microbiology

• ◯ Hygiene control
• ◯ Ready-to-use media
• ◯ Dried nutrient media
• ◯ Nutrient media supplements
• ◯ Culture media
• ◯ gelling agent
• ◯ antibiotics and antimycotics
• ◯ Cryopreservation

◯ Aids for histology and microscopy

• ◯ intermediates
• ◯ Reagents
• ◯ fixation
• ◯ embedding
• ◯ coloring
• ◯ containment
• ◯ Accessories for microscopy

◯ Auxiliary for cell biology

• ◯ Hygiene control
• ◯ Ready-to-use media
• ◯ Dried nutrient media
• ◯ Middle media supplements
• ◯ Tweezers
• ◯ Petri dishes
• ◯ Storage containers
  • ■ cell culture surfaces
  • ■ Cell culture bottles with filter screw cap, eg transparent
  • ■ Filters in the cell culture bottles
  • ■ Suspension culture bottles, made of eg PS, eg transparent
  • ■ cell culture multiwell plates
  • ■ With surface treatments such as DNase, RNase and / or human DNA-free
• ◯ adhesion slide
• ◯ Gloves, nitrilix and latex, powdered and unpowdered
• ◯ under gloves
• ◯ Disposable and reusable gloves
• ◯ Face masks and mouthguard, disposable and reusable, with filter and without filter
• ◯ filter of the mouth masks
• ◯ Work clothing, in particular lab coats, laboratory trousers, laboratory shoes
• ◯ Laboratory hair coverage and nets
• ◯ body suits and protective suits
• ◯ overshoes and shoe protectors
• ◯ Buttons for lab clothing
• ◯ Seal for microscopy
• ◯ Microscopy carrier

In particular, the following equipment and apparatus are suitable for coating with POM and / or with POM preparations.

• ◯ Mikrotiterplatten
• ◯ Lösemittelpumpen
• ◯ Pericyclische Pumpen
• ◯ Dispenser
• ◯ Pipetten
• ◯ Automatische Pipetten
• ◯ Rotatoren
• ◯ Mikrowelle
• ◯ Becher
• ◯ Schmelztiegel
• ◯ Schüttler
• ◯ Vortexer
• ◯ Kanister
• ◯ Auslaufhähne
• ◯ Mikroklingen
• ◯ Klingenhalter
• ◯ Gewebezangen
• ◯ Lupenbrillen
• ◯ Arbeitsplatzleuche
• ◯ Steriomikroskope
• ◯ Gluko-Sets
• ◯ Trichter, Büchnertricher
• ◯ Saugflaschen
• ◯ Spritzenfilter
• ◯ Vakuumpumpen
• ◯ Filtrations und Dialysesysteme
• ◯ Reaktionsgefässe
• ◯ Autotube-Racks
• ◯ Pipettierhilfen
• ◯ Kühlwasserwächter
• ◯ Stativmaterial wie Laborhebebühnen
• ◯ Steckdostenleisten
• ◯ Brutschränke
• ◯ Magnetische Aufbewahrungsbehälter
• ◯ Kombi-Racks
• ◯ Wägeschalen
• ◯ Mikrozentrifugen
• ◯ Kleinzentrifugen
• ◯ Reinigungs- und Pflegeaufsteller wie Wischtuschhalter,
• ◯ Ultraschallgeräte
• ◯ Magnesiarinnen und -stäbchen
• ◯ Siedesteinchen
• ◯ Wischtücher
• ◯ Laborhocker
• ◯ Etagenwagen
• ◯ Handkorb
• ◯ Gehörschutz
• ◯ Pflasterspender
• ◯ Augenduschen
• ◯ Sicherheitsabfallsysteme
• ◯ Entsorgungsbeutel
• ◯ Reaktionsgefäßständer
• ◯ Seifenspender
• ◯ Handtuchhalter

Articles and apparatus for the coating

• ◯ Microtiter plates
• ◯ Solvent pumps
• ◯ Pericyclic pumps
• ◯ Dispensers
• ◯ Pipettes
• ◯ Automatic pipettes
• ◯ rotators
• ◯ microwave
• ◯ cup
• ◯ crucible
• ◯ Shakers
• ◯ Vortexer
• ◯ canister
• ◯ drain taps
• ◯ microblades
• ◯ Blade holder
• ◯ tissue forceps
• ◯ Magnifying glasses
• ◯ Workplace light
• ◯ Steriomicroscopes
• ◯ Gluco sets
• ◯ Funnels, Büchnertricher
• ◯ feeding bottles
• ◯ Syringe filter
• ◯ Vacuum pumps
• ◯ Filtration and dialysis systems
• ◯ reaction vessels
• ◯ Car tube racks
• ◯ Pipetting aids
• ◯ Cooling water monitor
• ◯ Tripod material such as laboratory lifts
• ◯ socket strips
• ◯ incubators
• ◯ Magnetic storage containers
• ◯ Combi racks
• ◯ weighing bowls
• ◯ microcentrifuges
• ◯ small centrifuges
• ◯ cleaning and care stands such as wipe-dryers,
• ◯ Ultrasound equipment
• ◯ Magnesia and chopsticks
• ◯ boiling stones
• ◯ wipes
• ◯ laboratory stool
• ◯ Shelf trolley
• ◯ hand basket
• ◯ hearing protection
• ◯ plaster dispenser
• ◯ Eye showers
• ◯ safety waste systems
• ◯ disposal bag
• ◯ Reaction vessel stand
• ◯ Soap dispenser
• ◯ Towel rack

The above-mentioned laboratories, items, furniture, prints and apparatuses are exemplary and not exhaustive. The summary is intended to illustrate the various uses of the present invention. They are used primarily in the context of the use according to the invention, the cultures according to the invention, the kits according to the invention, the invention Proliferation method and the sterilization method according to the invention and the articles and fluids of the invention used.

However, everyday objects, apparatuses and installations can also be the subject of the use according to the invention, the cultures according to the invention, the kits according to the invention, the proliferation methods according to the invention, the sterilization method according to the invention and the articles and fluids according to the invention.

• ◯ Prothesen,
• ◯ Kopfhörer,
• ◯ Hörgeräte,
• ◯ Ohrstöpsel,
• ◯ Brillen,
• ◯ Sexspielzeuge,
• ◯ Toiletten,
• ◯ Schalter,
• ◯ Handys,
• ◯ Telefonhörer, Mobiltelefone, IPads, Laptops, PCs, Bildschirme
• ◯ Keyboards, Tastaturen
• ◯ Musikinstrumente jeder Art,
• ◯ Wände,
• ◯ Holz,
• ◯ Möbel jeglicher Art,
• ◯ Fliesen,
• ◯ Fußböden,
• ◯ Türen und Türgriffe,
• ◯ Geländer,
• ◯ Badutensilien wie Duschköpfe und Wasserhähne,
• ◯ medizinische und nicht medizinische Implantate,
• ◯ Glasrahmen,
• ◯ Schuhe und Schuheinlagen,
• ◯ Schutzkleidung,
• ◯ Laboratorien, insbesondere biologische und mikrobiologische Laboratorien,
• ◯ Zellkulturen,
• ◯ Gewebekulturen,
• ◯ Nährmedien,
• ◯ flüssige und feste, insbesondere pulverförmige, Vorprodukte für Zellkulturen, Gewebekulturen und Nährmedien,
• ◯ Krankenhäuser, insbesondere Operationssäle, Arztpraxen, medizinische Geräte,
• ◯ Imprägnierungen, die durch Aufsprühen eines POM-Mikro- und/oder - Nanopartikel enthaltenden Aerosols aufgetragen werden,
• ◯ Griffe an Einkaufswagen,
• ◯ Bestecke,
• ◯ Geschirr,
• ◯ sterile Folien und Behälter aus Glas, Metall oder Kunststoff, insbesondere für Zellkulturen, Getränke und Lebensmittel,
• ◯ Unterwasser-Antifouling-Ausrüstung,
• ◯ Fahrradgriffe und Motorradgriffe,
• ◯ das Innere von Transportmitteln wie Boote, Schiffe, Züge, Automobile, Lastwagen oder Flugzeuge,
• ◯ Innenräume im Allgemeinen, insbesondere in lebensmittelverarbeitenden Betrieben
• ◯ Klimaanlagen,
• ◯ Heizungen, insbesondere Öfen und Blockheizungen,
• ◯ Lüftungssysteme,
• ◯ befeuchtende oder entfeuchtende Raumbelüfter,
• ◯ kaschierte Folien, insbesondere aus Kunststoff,
• ◯ Glasuren für Keramiken,
• ◯ Füllstoffe, insbesondere Füllstoffe für Kissen, Polster oder Matratzen,
• ◯ Tone, Lehme und Böden,
• ◯ Beton, Baustoffe,
• ◯ Saatgut,
• ◯ Biosaatgut,
• ◯ Pflaster und Wundauflagen und Bandagen, einseitig und beidseitig,
• ◯ Silikon zur Abdichtung,
• ◯ Silikonspray,
• ◯ Antibaktereille Textilien,
• ◯ 3D-Printing Material,
• ◯ Antibakterielle Kunststoffe,
• ◯ Lacke und Oberflächenbeschichtungen,
• ◯ Keramiken,
• ◯ Fugenmaterial für Kacheln/Fliesen,
• ◯ Zusatz bei Medikamenten, um diese antibakteriell zu machen,
• ◯ Kombination mit Antispasmolytika und Cortikoiden zur Inhalation um multiresistente Baktereine und Viren z.B. bei der Lungenentzündung zu bekämpfen,
• ◯ Fischfarmen,
• ◯ Krebs und Lobsterfarmen,
• ◯ Insektenfarmen,
• ◯ Vorrichtungen für die Tierhaltung,
• ◯ Aquarien,
• ◯ Terrarien,
• ◯ Ställe,
• ◯ Gesichtsmasken, Mundschutz,
• ◯ Schwimmingpools,
• ◯ Antibakterielle Einlagen für Schuhe, Stiefel,
• ◯ Antibakterelle Zahlnbürsten,
• ◯ Lebensmittelverpackungen,
• ◯ Antibakterelle Papiere,
• ◯ Glasbeschichtungen,
• ◯ Tabs und Salzmischungen, die auch temperaturstabil sind,
• ◯ Waschtabs in Geschirrspülern,
• ◯ Autoinnenausstattung,
• ◯ Öffentliche Toiletten, Badezimmerzubehör,
• ◯ Putzmittel,
• ◯ Mülleimer,
• ◯ Textilspray für Textilien, die nicht gewaschen werden können.

The following are examples of such everyday items, equipment and equipment:

• ◯ prostheses,
• ◯ headphones,
• ◯ hearing aids,
• ◯ earplugs,
• ◯ glasses,
• ◯ sex toys,
• ◯ toilets,
• ◯ switches,
• ◯ cell phones,
• ◯ Telephone receivers, mobile phones, IPads, laptops, PCs, screens
• ◯ Keyboards, keyboards
• ◯ musical instruments of all kinds,
• ◯ walls,
• ◯ wood,
• ◯ furniture of any kind,
• ◯ tiles,
• ◯ floors,
• ◯ doors and door handles,
• ◯ railings,
• ◯ bathroom utensils such as shower heads and faucets,
• ◯ medical and non-medical implants,
• ◯ glass frame,
• ◯ shoes and shoe inserts,
• ◯ protective clothing,
• ◯ laboratories, in particular biological and microbiological laboratories,
• ◯ cell cultures,
• ◯ tissue cultures,
• ◯ nutrient media,
• ◯ liquid and solid, in particular pulverulent, precursors for cell cultures, tissue cultures and nutrient media,
• ◯ Hospitals, especially operating theaters, medical practices, medical devices,
• Impregnations applied by spraying a POM micro- and / or nanoparticle-containing aerosol,
• ◯ handles on shopping trolleys,
• ◯ cutlery,
• ◯ dishes,
• ◯ sterile films and containers made of glass, metal or plastic, in particular for cell cultures, drinks and food,
• ◯ underwater antifouling equipment,
• ◯ bicycle grips and motorcycle grips,
• ◯ the interior of means of transport such as boats, ships, trains, automobiles, trucks or aircraft,
• ◯ Interiors in general, especially in food processing establishments
• ◯ air conditioners,
• ◯ heaters, especially ovens and block heaters,
• ◯ ventilation systems,
• ◯ humidifying or dehumidifying room aerators,
• ◯ laminated films, in particular of plastic,
• ◯ glazes for ceramics,
• ◯ fillers, in particular fillers for cushions, upholstery or mattresses,
• ◯ clays, clays and soils,
• ◯ concrete, building materials,
• ◯ seed,
• ◯ organic seed,
• ◯ plasters and wound dressings and bandages, one-sided and two-sided,
• ◯ silicone for sealing,
• ◯ silicone spray,
• ◯ anti-bacterial textiles,
• ◯ 3D printing material,
• ◯ antibacterial plastics,
• ◯ paints and surface coatings,
• ◯ ceramics,
• ◯ jointing material for tiles / tiles,
• ◯ Adding medicines to make them antibacterial,
• Combination with anti-spasmolytics and corticosteroids for inhalation to combat multi-drug resistant bacteria and viruses eg in pneumonia,
• ◯ fish farms,
• ◯ Cancer and lobster farms,
• ◯ insect farms,
• ◯ devices for animal husbandry,
• ◯ aquariums,
• ◯ terrariums,
• ◯ stables,
• ◯ face masks, mouthguard,
• ◯ swimming pools,
• ◯ Antibacterial insoles for shoes, boots,
• ◯ Antibacterial number brushes,
• ◯ food packaging,
• ◯ Antibacterial papers,
• ◯ glass coatings,
• ◯ tabs and salt mixtures that are also temperature stable,
• ◯ wash tablets in dishwashers,
• ◯ car interior equipment,
• ◯ Public toilets, bathroom accessories,
• ◯ cleaning agents,
• ◯ dustbin,
• ◯ Textile spray for textiles that can not be washed.

• - für die Prophylaxe und/oder die Nachbehandlung von Mollicuteninfektionen und/oder Mollicutenkontaminationen, insbesondere Mykoplasmeninfektionen und/oder Mykoplasmenkontaminationen,
• - zur temporären oder dauerhaften Abtötung und/oder Dekontaminierung von Mollicutenkontaminationen, insbesondere Mykoplasmenkontaminationen,
• - zur temporären oder dauerhaften Inhibierung der Vermehrung von Mollicuten, insbesondere Mykoplasmen, und/oder
• - zur temporären oder dauerhaften Einstellung einer konstanten Konzentration von Mollicuten, insbesondere Mykoplasmen

von und in Viruskulturen, Kulturen einzelliger und mehrzelliger Eukaryoten sowie von und in Mikroorganismenpopulationen, wobei das mindestens eine Polyoxometallat höchstens in einer an Testkulturen ermittelten Grenzkonzentration angewandt.According to the invention, the extracellular and / or intracellular, preferably the extracellular and intracellular, use of at least one POM, at least one POM preparation and / or at least one mixture of at least two POM and / or at least two POM preparations

• for the prophylaxis and / or after-treatment of mollicin infections and / or mollicut contamination, in particular mycoplasma infections and / or mycoplasma contamination,
• for the temporary or permanent killing and / or decontamination of mollicut contaminants, in particular mycoplasma contamination,
• for the temporary or permanent inhibition of the proliferation of mollicutes, in particular mycoplasmas, and / or
• for the temporary or permanent setting of a constant concentration of Mollicut, especially Mycoplasma

from and in virus cultures, cultures of unicellular and multicellular eukaryotes and of and in microorganism populations, the at least one polyoxometalate being used at most in a limit concentration determined on test cultures.

The respective limit concentration is the concentration at which the at least one POM and / or the at least one POM preparation after an incubation time of a given eukaryotic test culture, virus test culture or test culture from a given microorganism population is from 0 hours to persistently, preferably 30 minutes to 100 days, preferably 30 minutes to 40 days and especially 30 minutes to 50 days and in particular 30 minutes to 10 days and after a Mykoplasendetektion after a life of 0 hours to permanent, preferably 30 minutes to 100 days, preferably 30 minutes to 50 Days and in particular 30 minutes to 10 days, the proliferation of the respective eukaryotes, the respective host cells of the virus or the microorganisms concerned not yet inhibited.

• - einzelligen und/oder mehrzelligen Eukaryoten,
• - Viren und
• - Mikroorganismenpopulationen

stammen von von den vorstehend beschriebenen Arzneimitteln, Zellkulturen, 3D-Zellkulturen, zellbiologischen Nährsystemen, zellbiologischen Nährmedien, Gewebekulturen, 3D-Gewebekulturen, Organen, Organtransplantaten, künstlich hergestellten Organen, Organen „on a chip“, Hilfsmedien, Laboratorien für die Biochemie, Molekularbiologie, Gentechnologie, Zellbiologie Mikrobiologie, Virologie, Pharmakologie, Toxikologie und Medizin sowie von Gegenständen, Kleidung, Gerätschaften und Apparaturen für und in diesen Laboratorien.The cultures of

• - unicellular and / or multicellular eukaryotes,
• - viruses and
• - Microorganism populations

are derived from the medicines described above, cell cultures, 3D cell cultures, cell biological nutrient systems, cell culture media, tissue cultures, 3D tissue cultures, organs, organ transplants, man-made organs, on-chip organs, auxiliary media, biochemistry laboratories, molecular biology, Genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology and medicine, and articles, clothing, equipment and apparatus for and in these laboratories.

Preferably, the respective determined in each case limit concentrations of at least one POM at an incubation time of an infected test culture of 30 minutes with a Mollicutendetektion, especially Mycoplasma detection, after another 5 days life <15 mM and the limit concentration of the at least one polyoxometalate for an incubation period of 4 days with a Mollicut dispersion, in particular Mycoplasma detection, after a further 5 days' life <1.0 mM.

Preferably, the at least one POM as described above is selected from the group consisting of unaltered, oxygenated, functionalized, coated, grafted, aggregated, agglomerated, and supported POM molecules of largest molecular diameter ≤2 nm and POM nanoparticles and POM microparticles average particle size of 1 nm to <1000 microns selected.

• - in Form einer Säure, eines Salzes, eines Hydrats, eines Soft-Oxometallats (SOMS), einer Beschichtung und/oder eines Polyoxometallat-Nanocomposits, und/oder
• - in molekulardispers gelöster, gepufferter, suspendierter, lipophilisierter und/oder mit organischen Gruppen fluidisierter Form und/oder
• - in und/oder auf als sich dispergierenden, teilweise auflösenden, nicht auflösenden oder völlig auflösenden, kompakten und/oder porösen Partikeln, Beschichtungen, Coatings, Releasematerialien, Schwämmchen, Plättchen, Folienstückchen, Textilstückchen, Gewebestückchen, Cellulosefasern, Cellulosemikropartikeln, Cellulosenanopartikeln, Nanosomen, Mikrosomen, Liposomen, Tabletten, Glaskügelchen, Sprühgeräten, Verneblern und/oder als Beschichtung, die die Oberfläche hart und glatt und/oder diffusionsgeschlossen macht, und/oder
• - in Aerosolen, Nebeln, Hydrogelen, in Micellen, in Vesikeln, in Mikrokapseln, in Mikrosphären, in Gelen, in Cremes, Lotions, Salben und/oder Schäumen und/oder
• - in Verbindung mit funktionalen Materialien, Antibiotika, bioziden Materialien und/oder Nährmedien und/oder Hilfsmedien für die Zellbiologie und/oder
• - in Form seiner Ausgangsprodukte, die sich spontan zu dem mindestens einen Polyoxometallat zusammenfügen,

vorliegen.The at least one POM may be present in at least one POM preparation

• in the form of an acid, a salt, a hydrate, a soft oxometalate (SOMS), a coating and / or a polyoxometalate nanocomposite, and / or
• - in molecularly dispersed, buffered, suspended, lipophilized and / or fluidized with organic groups form and / or
• in and / or on as dispersing, partially dissolving, non-dissolving or completely dissolving, compact and / or porous particles, coatings, coatings, release materials, sponges, platelets, foil pieces, textile pieces, fabric pieces, cellulose fibers, cellulose microparticles, cellulose nanoparticles, nanosomes, Microsomes, liposomes, tablets, glass beads, sprayers, nebulizers and / or as a coating that makes the surface hard and smooth and / or diffusion-closed, and / or
• in aerosols, mists, hydrogels, micelles, vesicles, microcapsules, microspheres, gels, creams, lotions, ointments and / or foams and / or
• - in conjunction with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary media for cell biology and / or
• in the form of its starting materials, which spontaneously combine to form the at least one polyoxometalate,

available.

• - Ammoniumheptamolybdat-Tetrahydrat {(NH₄)₆Mo₇O₂₄] · 4H₂O, CAS-Nr. 13106-76-8 (wasserfrei), CAS-Nr. 12054-85-2 (Tetrahydrat), AHMT},
• - Wolframatophosphorsäure-Hydrat {H₃[P(W₃O₁₀)₄] · xH₂O, CAS-Nr. 1343-93-7 (wasserfrei), CAS-Nr. 12067-99-1 (Hydrat), Wo-Pho},
• - Molybdatophosphorsäure-Hydrat, {H₃P(Mo₃O₁₀)4] · xH2O, CAS-Nr.:12026-57-2 (wasserfrei), CAS-Nr. 51429-74-4 (Hydrat), Mo-Pho} und
• - Wolframatokieselsäure {H₄[Si(W₃O₁₀)₄] · xH₂O, CAS-Nr. 12027-43-9, CAS-Nr. WKS}

ausgewählt.Most preferably, the at least one POM is selected from the group consisting of

• - Ammonium heptamolybdate tetrahydrate {(NH ₄ ) ₆ Mo ₇ O ₂₄ ] · 4H ₂ O, CAS no. 13106-76-8 (anhydrous), CAS no. 12054-85-2 (tetrahydrate), AHMT},
• Tungstophosphoric hydrate {H ₃ [P (W ₃ O ₁₀ ) ₄ ] .xH ₂ O, CAS-No. 1343-93-7 (anhydrous), CAS no. 12067-99-1 (hydrate), Wo-Pho},
• - Molybdatophosphoric hydrate, {H ₃ P (Mo ₃ O ₁₀ ) 4] · xH ₂ O, CAS No.:12026-57-2 (anhydrous), CAS-No. 51429-74-4 (hydrate), Mo-Pho} and
• Tungstosilicic acid {H ₄ [Si (W ₃ O ₁₀ ) ₄ ] .xH ₂ O, CAS no. 12027-43-9, CAS no. WKS}

selected.

Preferably, the eukaryotes are the viruses and microorganism populations of the infected cultures of vertebrates, deuterostomes, proterostomes, tissue animals, multicellulars, unicellulars, plants, algae and / or arthropods.

Preferably, the eukaryotes, viruses and microorganism populations of the infected cultures are from humans, mammals, birds, reptiles, amphibians, fish, bony fish, cartilaginous, non-jawed, echinoderms, annelids, centipedes, crustaceans, arachnids, insects, molluscs, tubeworms, flatworms, Hollow animals, sponges, protists and algae.

The term "derived from" is to be understood in the sense that the eukaryotes are derived from the animals as such, i. Cells of these organisms are, whereas the viruses and the microorganism populations populate the said organisms and are taken in the form of samples.

Both the eukaryotes and the viruses and the microorganism populations may become strong within a same species depending on infestation with diseases, bacteriophages, toxicants, noxae, etc. differ from each other. For this very reason, pure, mollicut-free, in particular mycoplasma-free, cultures are so important for the corresponding investigations.

• - von Arzneimitteln, zellbiologischen Nährsystemen, zellbiologischen Nährmedien, Gewebekulturen, 3D-Gewebekulturen, Organen, Organtransplantaten, künstlich hergestellten Organen, Organen „on a chip“, Hilfsmedien, Laboratorien für die Biochemie, Molekularbiologie, Gentechnologie, Zellbiologie Mikrobiologie, Virologie, Pharmakologie, Toxikologie und Medizin sowie von Gegenständen, Kleidung, Gerätschaften und Apparaturen für und in diesen Laboratorien stammen, wobei
• - das mindestens eine POM in einer an Testkulturen ermittelbaren Grenzkonzentration vorliegt, in der das mindestens eine POM nach einer Inkubationzeit einer gegebenen Eukaryotenkultur, einer gegebenen Viruskultur oder einer von einer gegebenen Mikroorganismenpopulation stammenden Kultur von 0 Stunden bis auf Dauer, vorzugsweise 30 Minuten bis 100 Tagen, bevorzugt 30 Minuten bis 50 Tagen und insbesondere 30 Minuten bis 10 Tagen und einer Mollicutendetektion, insbesondere Mykoplasmendetektion, nach einer Standzeit von 0 Stunden bis auf Dauer, vorzugsweise 30 Minuten bis 100 Tagen und insbesondere 30 Minuten bis 50 Tagen und insbesondere 30 Minuten bis 10 Tagen für die Proliferation der betreffenden Eukaryoten, der betreffenden Wirtszellen der Viren oder der betreffenden Mikroorganismen noch nicht inhibierend ist.

The proliferative Mollicut-free, at least one polyoxometalate-free cultures of unicellular and / or multicellular eukaryotes, virus cultures and cultures of microorganism populations according to the invention obtained according to the invention are, in particular, cultures

• - of pharmaceuticals, cell biological nutrient systems, cell culture media, tissue cultures, 3D tissue cultures, organs, organ transplants, man-made organs, on-chip organs, auxiliary media, biochemistry laboratories, molecular biology, genetic engineering, cell biology microbiology, virology, pharmacology, toxicology and medicine, and articles, clothing, equipment and apparatus for and in these laboratories, wherein
• - the at least one POM is present in a limit concentration determinable at test cultures in which the at least one POM after incubation of a given eukaryotic culture, virus culture or culture from a given microorganism population is from 0 hours to persistently, preferably 30 minutes to 100 days , preferably 30 minutes to 50 days and especially 30 minutes to 10 days and a Mollicutendetektion, in particular mycoplasma detection, after a period of 0 hours to persist, preferably 30 minutes to 100 days and especially 30 minutes to 50 days and especially 30 minutes to 10 Days for the proliferation of the eukaryotes concerned, the relevant host cells of the virus or the microorganisms in question is not yet inhibitory.

For the use according to the invention, it is of particular advantage to provide the kits according to the invention.

The kits according to the invention contain at least one device for carrying out customary and known spectroscopic, microbiological and / or chemical measurements for the qualitative and / or quantitative detection of unicellular eukaryotes, multicellular eukaryotes, host cells of viruses, microorganism populations and mollicuts, in particular mycoplasma, on respectively at least one test sample which is taken from at least one test culture infected with at least one kind of mollicut, in particular mycoplasma, containing at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes, at least one kind of host cells or at least one kind of microorganism populations.

Furthermore, the kits according to the invention contain at least one device for evaluating and storing the test results measured on the at least one test sample.

In addition, the kits according to the invention comprise at least one type of mollicut-free microbiological nutrient media, nutrient systems, auxiliary media, equipment and apparatuses for producing at least one culture free of mycoplasma, at least one species free from mycoplasma of unicellular eukaryotes and / or at least one type of multicellular eukaryote, at least one type of host cell for viruses or at least one type of microorganism population. This culture serves as a null sample, wherein the eukaryotes, viruses or microbial population of drugs, cell cultures, 3D cell cultures, cell biological nutrient systems, cell culture media, tissue cultures, 3D tissue cultures, organs, organ transplants, artificially prepared organs, organs "on a chip ", auxiliary media and smears from laboratories for cell biological nutrient systems, cell culture media, tissue cultures, 3D tissue cultures, organs, organ transplants, man-made organs, on-chip organs, auxiliary media, biochemistry laboratories, molecular biology, genetic engineering, cell biology microbiology , Virology, Pharmacology, Toxicology and Medicine as well as objects, clothing, utensils, furniture, prints, equipment for and in these laboratories.

Furthermore, the kits according to the invention contain cell-biological nutrient media, cell-biological nutrient systems, auxiliary media, equipment and apparatus for producing at least one cell biological standard preparation with a precisely known dose of at least one type of unicellular eukaryotes and / or at least one type of multicellular eukaryotes, at least one type of host cell Viruses or at least one species of microorganism populations infected with a well known dose of at least one species of mollicut, in particular mycoplasma.

Furthermore, the kits according to the invention contain at least one preparation of a precisely known dose of at least one POM and at least one dosing device for the at least one preparation of a precisely known dose of at least one POM.

Preferably, in the kits of the invention, the at least one POM is selected from the group consisting of unaltered, oxygen-substituted, functionalized, coated, grafted, aggregated, agglomerated, and supported POM molecules of largest molecular diameter ≤2 nm, and POM nanoparticles and POM microparticles of a middle molecular weight Particle size from 1 nm to <1000 microns selected.

• - in Form einer Säure, eines Salzes, eines Hydrats, eines Soft-Oxometallats (SOMS), einer Beschichtung und/oder eines Polyoxometallat-Nanocomposits, und/oder
• - in molekulardispers gelöster, gepufferter, suspendierter, lipophilisierter und/oder mit organischen Gruppen fluidisierter Form und/oder
• - in und/oder auf als sich dispergierenden, teilweise auflösenden, nicht auflösenden oder völlig auflösenden, kompakten und/oder porösen Partikeln, Beschichtungen, Coatings, Releasematerialien, Schwämmchen, Plättchen, Folienstückchen, Textilstückchen, Gewebestückchen, Cellulosefasern, Cellulosemikropartikeln, Cellulosenanopartikeln, Nanosomen, Mikrosomen, Liposomen, Tabletten, Glaskügelchen, Sprühgeräten, Verneblern und/oder als Beschichtung, die die Oberfläche hart und glatt und/oder diffusionsgeschlossen macht, und/oder
• - in Aerosolen, Nebeln, Hydrogelen, in Micellen, in Vesikeln, in Mikrokapseln, in Mikrosphären, in Gelen, in Cremes, Lotions, Salben und/oder Schäumen und/oder
• - in Verbindung mit funktionalen Materialien, Antibiotika, bioziden Materialien und/oder Nährmedien und/oder Hilfsmedien für die Zellbiologie und/oder
• - in Form seiner Ausgangsprodukte, die sich spontan zu dem mindestens einen Polyoxometallat zusammenfügen,

verwendet.The at least one POM and / or the at least one POM preparation is preferred in the kits according to the invention

• in the form of an acid, a salt, a hydrate, a soft oxometalate (SOMS), a coating and / or a polyoxometalate nanocomposite, and / or
• - in molecularly dispersed, buffered, suspended, lipophilized and / or fluidized with organic groups form and / or
• in and / or on as dispersing, partially dissolving, non-dissolving or completely dissolving, compact and / or porous particles, coatings, coatings, release materials, sponges, platelets, foil pieces, textile pieces, fabric pieces, cellulose fibers, cellulose microparticles, cellulose nanoparticles, nanosomes, Microsomes, liposomes, tablets, glass beads, sprayers, nebulizers and / or as a coating that makes the surface hard and smooth and / or diffusion-closed, and / or
• in aerosols, mists, hydrogels, micelles, vesicles, microcapsules, microspheres, gels, creams, lotions, ointments and / or foams and / or
• - in conjunction with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary media for cell biology and / or
• in the form of its starting materials, which spontaneously combine to form the at least one polyoxometalate,

used.

• - Ammoniumheptamolybdat-Tetrahydrat {(NH₄)₆Mo₇O₂₄] · 4H₂O, CAS-Nr. 13106-76-8 (wasserfrei), CAS-Nr. 12054-85-2 (Tetrahydrat), AHMT},
• - Wolframatophosphorsäure-Hydrat {H₃[P(W₃O₁₀)₄] · xH₂O, CAS-Nr. 1343-93-7 (wasserfrei), CAS-Nr. 12067-99-1 (Hydrat), Wo-Pho},
• - Molybdatophosphorsäure-Hydrat, {H₃P(Mo₃O₁₀)4] · xH2O, CAS-Nr.:12026-57-2 (wasserfrei), CAS-Nr. 51429-74-4 (Hydrat), Mo-Pho} und
• - Wolframatokieselsäure {H₄[Si(W₃O₁₀)₄] · xH₂O, CAS-Nr. 12027-43-9, CAS-Nr. WKS}.

In particular, the kits according to the invention contain at least one POM from the group consisting of

• - Ammonium heptamolybdate tetrahydrate {(NH ₄ ) ₆ Mo ₇ O ₂₄ ] · 4H ₂ O, CAS no. 13106-76-8 (anhydrous), CAS no. 12054-85-2 (tetrahydrate), AHMT},
• Tungstophosphoric hydrate {H ₃ [P (W ₃ O ₁₀ ) ₄ ] .xH ₂ O, CAS-No. 1343-93-7 (anhydrous), CAS no. 12067-99-1 (hydrate), Wo-Pho},
• - Molybdatophosphoric hydrate, {H ₃ P (Mo ₃ O ₁₀ ) 4] · xH ₂ O, CAS No.:12026-57-2 (anhydrous), CAS-No. 51429-74-4 (hydrate), Mo-Pho} and
• Tungstosilicic acid {H ₄ [Si (W ₃ O ₁₀ ) ₄ ] .xH ₂ O, CAS no. 12027-43-9, CAS no. WKS}.

In a particularly preferred embodiment, the kits according to the invention are controlled by a data processing system, automated robots. In a most preferred embodiment, the automated robots include automated peripherals with automatic dosages for the materials described above, for transporting the materials, utensils, samples, well plates or microtiter plate, to the respective measurement sites, incubators and equipment for the appropriate Disposal of crops and chemicals.

In the inventive method (i) for the decontamination of mollicuteninfiziert, especially mycoplasma infected, eukaryotic cultures, virus cultures and cultures of microorganism populations and (ii) for the proliferation of decontaminated, mollicutenfreien, in particular mycoplasma-free, cultures of at least one mollicutenfreien, especially mycoplasma free, and / or at least one molliculturally infected, in particular mycoplasma-infected test culture of at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes, at least one kind of host cells for viruses or at least one kind of microorganism populations, the limit concentration at least a POM for inhibiting the proliferation of at least one type of eukaryotes, host cells for viruses or microorganism populations.

Subsequently, at least one culture of at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes, of at least one kind of host cells for viruses or of at least one kind of microorganism populations, whose limiting concentration of the at least one POM is used, is at least one mollicutin-infected, in particular mycoplasma-infected culture Inhibition of proliferation has been determined after the previous step, with the at least one POM offset, so that in the at least one mollicuteninfiziert, in particular mycoplasma -infected, culture at most results in the limit concentration of the at least one POM

Thereafter, the resulting at least one mollicuteninfiziert, especially mycoplasma infected culture for 0 hours to permanent, preferably 30 minutes to 100 days, preferably 30 minutes to 50 days and especially 30 minutes to 10 days incubated and a Mollicutendetektion, in particular a Mykoplasmendetektion, after a Life of 0 hours to permanent, preferably 30 minutes to 100 days, preferably 30 minutes to 50 days and especially 30 minutes to 10 days determined whether even Mollicutes, especially mycoplasma, are present.

If this is not the case, at least a portion of the at least one now mollicutenfreien, in particular mycoplasma-free culture of at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes, at least one type of host cell for viruses or at least one kind of microorganism populations to at least one mollicutenfreies, in particular mycoplasma-free, nutrient medium transmits and the at least one species of mollicut free, in particular free from mycoplasma, eukaryotes, host cells and microorganism populations in the resulting at least second culture.

The eukaryotes, the viruses with their cell cultures or the microorganism populations in the above-mentioned sense are also derived from medicaments, cell cultures, 3D cell cultures, cell biological nutrient systems, cell biological culture media, tissue cultures, 3D tissue cultures, organs, organ transplants, artificially produced organs. "On-chip" and / or auxiliary media and laboratories for biochemistry, molecular biology, genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology and medicine and of articles, clothing, utensils, furniture, prints, apparatus and laboratory personnel for and in these laboratories.

Preferably, this inventive method is carried out automatically using the robot kit described above.

• - in Form einer Säure, eines Salzes, eines Hydrats, eines Soft-Oxometallats (SOMS), einer Beschichtung und/oder eines Polyoxometallat-Nanocomposits, und/oder
• - in molekulardispers gelöster, gepufferter, suspendierter, lipophilisierter und/oder mit organischen Gruppen fluidisierter Form und/oder
• - in und/oder auf als sich dispergierenden, teilweise auflösenden, nicht auflösenden oder völlig auflösenden, kompakten und/oder porösen Partikeln, Beschichtungen, Coatings, Releasematerialien, Schwämmchen, Plättchen, Folienstückchen, Textilstückchen, Gewebestückchen, Cellulosefasern, Cellulosemikropartikeln, Cellulosenanopartikeln, Nanosomen, Mikrosomen, Liposomen, Tabletten, Glaskügelchen, Sprühgeräten, Verneblern und/oder als Beschichtung, die die Oberfläche hart und glatt und/oder diffusionsgeschlossen macht, und/oder
• - in Aerosolen, Nebeln, Hydrogelen, in Micellen, in Vesikeln, in Mikrokapseln, in Mikrosphären, in Gelen, in Cremes, Lotions, Salben und/oder Schäumen und/oder
• - in Verbindung mit funktionalen Materialien, Antibiotika, bioziden Materialien und/oder Nährmedien und/oder Hilfsmedien für die Zellbiologie und/oder
• - in Form seiner Ausgangsprodukte, die sich spontan zu dem mindestens einen Polyoxometallat zusammenfügen,

die auf und/oder in die Gegenstände und/oder in die Fluide

• - in einer an Eukaryotentestkulturen, Virustestkulturen oder Testkulturen von Mikroorganismenpopulationen ermittelbaren Grenzkonzentration eingebracht ist oder sind,
• - bei der das mindestens eine POM und/oder die mindestens eine POM-Präparation nach einer Inkubation der Testkulturen während 0 Stunden bis auf Dauer, vorzugsweise 30 Minuten bis 100 Tagen, bevorzugt 30 Minuten bis 50 Tagen und insbesondere 30 Minuten bis 10 Tagen und einer Mollicutendetektion, insbesondere Mykoplasmendetektion, nach einer Standzeit der inkubierten Testulturen von 0 Stunden bis auf Dauer, vorzugsweise 30 Minuten bis 100 Tagen, bevorzugt 30 Minuten bis 50 Tagen und insbesondere 30 Minuten bis 10 Tagen für die Proliferation der betreffenden Eukaryoten, der betreffenden Wirtszellen und Viren oder der betreffenden Mikroorganismen noch nicht inhibierend ist oder sind, wobei die Eukaryoten, die Viren und die Mikroorganismenpopulationen der Testkulturenvon den Gegenständen und/oder von den Fluiden stammen.

The use according to the invention also makes it possible to provide objects according to the invention protected against mollicut infestation, in particular mycoplasma infestation, and articles which contain at least one POM and / or at least one POM

• in the form of an acid, a salt, a hydrate, a soft oxometalate (SOMS), a coating and / or a polyoxometalate nanocomposite, and / or
• - in molecularly dispersed, buffered, suspended, lipophilized and / or fluidized with organic groups form and / or
• in and / or on as dispersing, partially dissolving, non-dissolving or completely dissolving, compact and / or porous particles, coatings, coatings, release materials, sponges, platelets, foil pieces, textile pieces, fabric pieces, cellulose fibers, cellulose microparticles, cellulose nanoparticles, nanosomes, Microsomes, liposomes, tablets, glass beads, sprayers, nebulizers and / or as a coating that makes the surface hard and smooth and / or diffusion-closed, and / or
• in aerosols, mists, hydrogels, micelles, vesicles, microcapsules, microspheres, gels, creams, lotions, ointments and / or foams and / or
• - in conjunction with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary media for cell biology and / or
• in the form of its starting materials, which spontaneously combine to form the at least one polyoxometalate,

on and / or in the articles and / or in the fluids

• is or is introduced in a limit concentration determinable on eukaryotic test cultures, virus test cultures or test cultures of microorganism populations,
• - in which the at least one POM and / or the at least one POM preparation after incubation of the test cultures for 0 hours to persist, preferably 30 minutes to 100 days, preferably 30 minutes to 50 days and especially 30 minutes to 10 days and a Mollicutendetektion, in particular mycoplasma detection, after a lifetime of the incubated test cultures from 0 hours to permanent, preferably 30 minutes to 100 days, preferably 30 minutes to 50 days and especially 30 minutes to 10 days for the proliferation of the eukaryotes in question, the host cells and viruses concerned or the microorganisms in question are or are not yet inhibitory, the eukaryotes, viruses and microorganism populations of the test cultures being from the articles and / or from the fluids.

As objects and fluids, which are protected against Mollicutenbefall, in particular Mycoplasma attack, all the above-described objects and fluids come into consideration. These are given by way of example and not exhaustively in the present application and are intended to substantiate the surprisingly broad applicability of the use according to the invention.

Due to the use according to the invention, the conditions for the decontamination and sterilization of medicaments, cell biological nutrient systems, cell biological nutrient media and of laboratories for biochemistry, molecular biology, genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology and medicine and of objects can now also be considered For this purpose, at least one cleaning agent containing at least one POM and / or at least one POM preparation in a minimum dosage> the limiting concentration as described above must be used ,

In the following, the invention and the advantages achieved thereby will be explained in more detail by means of examples and comparative experiments. The examples do not limit the invention, but serve to illustrate it.

Examples and Comparative Experiments

example 1

The decontamination of mycoplasma-infected cultures of unicellular and multicellular eukaryotes and the proliferation of mycoplasma-free unicellular and multicellular eukaryotes in a schematic representation

The figure shows a possible embodiment of the procedure for decontamination and the proliferation as a flow chart in a schematic representation.

1

einzellige oder mehrzellige Eukaryoten

2

Mykoplasmen

In

Inkubation

G

Gerät für die Zellbiologie

MT

Mykoplasmen-Test

N

Mykoplasmenfreies Nährmedium

O

Oberfläche von G

P1-Pn

Sterilisierte Mikrotiterplatten

POM1

Niedrigste Konzentration von Polyoxometallat im Nährmedium N

POM2

Höhere Konzentration von Polyoxometallat im Nährmedium N

POM3

Höchste Konzentration von Polyoxometallat im Nährmedium N

Pr

Proliferation

S12

Mykoplasmeninfizierte Eukaryoten 1 enthaltender Oberflächenfilm

Tr

Transfer.

In the figure, the reference numerals have the following meaning:

1

unicellular or multicellular eukaryotes

2

mycoplasma

In

incubation

G

Device for cell biology

MT

Mycoplasma test

N

Mycoplasma-free nutrient medium

O

Surface of G

P1-Pn

Sterilized microtiter plates

POM1

Lowest concentration of polyoxometalate in the nutrient medium N

POM2

Higher concentration of polyoxometalate in the nutrient medium N

POM3

Highest concentration of polyoxometalate in the nutrient medium N

pr

proliferation

S12

Mycoplasma-infected eukaryotes 1 containing surface film

Tr

Transfer.

Determination of the limit concentration of polyoxometalates for the decontamination of mycoplasmas 2

It was in three sterilized microtiter plates P1 . P2 and P3 (symbolized by a circle) on a mycoplasma-free nutrient medium N three cultures of a species of eukaryotes 1 each set in the same number and incubated ( In ). After proliferations (Pr), the culture became that of the microtiter plate P1 with polyoxometalate POM1 vaccinated in a first, low concentration. The culture in the microtiter plate P2 was given a second, slightly higher concentration of polyoxometalate POM2 vaccinated. The culture in the microtiter plate P3 was the highest concentration of polyoxometalate POM3 vaccinated. The three vaccinated cultures were incubated again ( In ). It turned out that the two cultures in the microtiter plates P1 and P2 had increased, whereas the culture in the third microtiter plate P3 had not multiplied. In other words, the concentration of polyoxometalate was equivalent POM3 the limit concentration above which a significant inhibition of the proliferation of the examined eukaryotic species had occurred. It could be concluded that the limit concentration of polyoxometalate POM for the decontamination of cultures of the type of eukaryotes used 1 equal to or below this third concentration POM3 had to stay.

Decontamination of a mycoplasma 2 infected culture of eukaryotes 1 and proliferation of eukaryotes 1

From the surface O of a device G for the microbiological laboratory became a sample of a microorganism population S12 that with mycoplasma 2 infected eukaryotes 1 contained, taken and on the nutrient medium N the microtiter plate P4 transferred. The presence of mycoplasma 2 was verified using the MilliPROBE® system from Merck ( MT ). Subsequently, the colony was incubated ( In ), which causes the mycoplasmas 2 and the eukaryotes 1 multiplied ( pr . MT ). The culture with the increased population of mycoplasmas 2 and eukaryotes 1 in the microtiter plate P4 was mixed with the polyoxometalate in a limiting concentration between POM 2 and POM 3 lay, staggered and incubated again ( In ). Subsequently, the MilliPROBE® system was used to eliminate mycoplasma 2 in the culture of the microtiter plate P4 detected. The of mycoplasma 2 free culture of eukaryotes 1 was incubated ( In ) and multiplies ( pr ). The culture of the increased eukaryotes 1 in P4 was again tested for mycoplasma ( MT ). The culture proved to be free of mycoplasma 2 , Parts of the culture of eukaryotes 2 in P4 could now on more nutrient media N in the microtiter plates P5 transferred to Pn ( Tr ) and by incubation ( In ).

The increased mycoplasma-free eukaryotes in this way 1 could be safely identified and subjected to testing. Thereafter, it was possible to search specifically for the source of contamination in the microbiological laboratory. In addition, the surface could O of the device G with a cleaning solution containing polyoxometalate, as described in the German patent application DE 10 2015 000 812.5 Examples 1 and 2 will be disinfected. It is important to ensure that the concentration of polyoxometalate is higher than the limit concentration determined above.

In order to rule out that the benefits and effects found are partly or entirely attributable to daylight, the tests were carried out in the dark and in daylight. No differences were found, which is why the advantages and effects are due solely to the polyoxometalates.

Example 2

The influence of polyoxometalates on the fluorescence of media

The POM showed differences in fluorescence. This had to be considered when working with fluorescence reagents. At the same time, however, this could be used as an advantage to directly indicate the activity against mycoplasma in the emission spectrum.

For fluorescence measurements, a DMEM medium including 10% FCS (fetal calf serum, 4 mM L-glutamate and 1 mM sodium pyruvate) was used and the results were summarized in the following tables. Media emission signals with different concentrations of molybdophosphoric hydrate (Mo-Pho) and tungstophosphoric acid hydrate (Wo-Pho) when excited with light of wavelength 594 nm Medium AHMT concentration Fluorescence intensity emission signal at 630 nm 0 1443 P / s ^a) 1471 80.9 μM 1543 0.4 mM 1414 0.8mM 1342 1.6 mM 1328 4.9 mM 1285 9.7 mM 700 19.4 mM 628 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza

From a concentration of 0.4 mM, there is a gradual reduction of the emission signal with increasing AHMT concentration. Table 7: Emission signals of the media with different ammonium heptamolybdate tetrahydrate (AHMT) concentrations when excited with light of a wavelength of 555 nm Medium AHMT concentration Fluorescence intensity emission signal at 555 nm 0 1514 P / S ^a) 1542 80.9 μM 1628 0.4 mM 1500 0.8mM 1428 1.6 mM 1314 2.4 mM 1028 9.7 mM 857 19.4 mM 714 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza

Again, from a concentration of 0.8 mM, there is a gradual reduction of the emission signal with increasing AHMT concentration. Table 8: Emission signals of the media with different ammonium heptamolybdate tetrahydrate (AHMT) concentrations when excited with light of a wavelength of 488 nm Medium AHMT concentration Fluorescence intensity emission signal at 530 nm 0 7000 P / S ^a) 6500 80.9 μM 7125 0.4 mM 7000 0.8mM 7187 1.6 mM 7312 2.4 mM 5500 9.7 mM 5125 19.4 mM 4437 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza

Here, too, from a concentration of 1.6 mM, there is a gradual reduction of the emission signal with increasing AHMT concentration. Table 9: Emission signals from the media with different concentrations of molybdophosphoric hydrate (Mo-Pho) and tungstophosphoric acid hydrate (Wo-Pho) when excited with light of 555 nm wavelength POM Medium POM concentration Fluorescence intensity emission signal at 585 nm - 0 10714 - P / S ^a) 10,000 Mo Pho 0.05mM 9285 Mo Pho 0.5 mM 6428 Mo Pho 1 mm 5714 Wo-Pho 0.01 mM 10357 Wo-Pho 0.1 mM 10,200 Wo-Pho 0.5 mM 8571 Milli Q water - near 0 Ko 594 ^b) 0.07 μg / ml 17857 Ko 594 ^b) 0.2 μg / ml 31071 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza; b) KnockOut ™ DMEM Thermo Fisher Scietific

The fluorescence intensity of the emission signal at 630 nm was higher in the case of tungstophosphoric acid (eg 0.01 mM) even at a lower concentration than in the case of molybdophosphoric acid (eg 0.05 mM). Table 10: Emission signals from the media with different concentrations of molybdophosphoric hydrate (Mo-Pho) and tungstophosphoric acid hydrate (Wo-Pho) when excited with light of 555 nm wavelength POM Medium POM concentration Fluorescence intensity emission signal at 585 nm - 0 5137 - P / S ^a) 5625 Mo Pho 0.05mM 5312 Mo Pho 0.5 mM 3750 Mo Pho 1 mm 3125 Wo-Pho 0.01 mM 5625 Wo-Pho 0.1 mM 5625 Wo-Pho 0.5 mM 5000 Milli Q water - 0 Ko 594 ^b) 0.07 μg / ml 11875 Ko 594 ^b) 0.2 μg / ml 12312 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza; b) KnockOut ™ DMEM Thermo Fisher Scietific

The fluorescence intensity of the emission signal at 630 nm was higher with tungstophosphoric acid (eg 0.01 mM) even at a lower concentration than with molybdophosphoric acid (eg 0.05 mM). Table 11: Emission signals of the media with different concentrations of molybdophosphoric acid hydrate (Mo-Pho) and tungstophosphoric acid hydrate (Wo-Pho) when excited with a wavelength of 488 nm POM Medium POM concentration Fluorescence intensity emission signal at 530 nm - 0 17,000 - P / S ^a) 12,000 Mo Pho 0.05mM 13,000 Mo Pho 0.5 mM 15,000 Mo Pho 1 mm 14,000 Wo-Pho 0.01 mM 12,000 Wo-Pho 0.1 mM 14,000 Wo-Pho 0.5 mM 14,000 Milli Q water - 0 Ko 594 ^b) 0.07 μg / ml 47,000 Ko 594 ^b) 0.2 μg / ml 86,000 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza; b) KnockOut ™ DMEM Thermo Fisher Scietific

The fluorescence intensity of the emission signal at 630 nm was higher with tungstophosphoric acid (e.g., 0.01 mM) even at lower concentration than with molybdophosphoric acid (e.g., 0.05 mM).

Example 3

The interactions of POM with reacted and unreacted WST 8th and LDH

It was investigated whether the absorption of POM is the usual color change based assay for determining cell viability with the MTT assay with 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide ( WST-8) or the test with L-lactate dehydrogenase (LDH). For this purpose, the interference of the POM with the reacted and unreacted WST-8 reagent and with the reacted and unreacted LDH reagent were measured. Table 12: Interference of the POM with the reacted WST-8 reagent POM Medium POM concentration absorption - 0 2.53 - Triton 0.01% 2.49 0 - P / S ^a) 2.5 Mo Pho 0.5 mM 2.47 Mo Pho 1 mm 2.51 Mo Pho 4mM 2.53 Wo-Pho 0.5 mM 2.33 Wo-Pho 1 mm 2.56 Wo-Pho 4mM 2.56 AHMT 0.1 mg / ml 2.49 AHMT 1 mg / ml 2.51 AHMT 6 mg / ml 2.52 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza Table 13: Interference of the POM with the unreacted WST-8 reagent POM Medium POM concentration absorption - 0 0.17 - Triton 0.01% 0.17 0 - P / S ^a) 0.15 Mo Pho 0.5 mM 0.15 Mo Pho 1 mm 0.15 Mo Pho 4mM 0.13 Wo-Pho 0.5 mM 0.15 Wo-Pho 1 mm 0.16 Wo-Pho 4mM 0.14 AHMT 0.1 mg / ml 0.15 AHMT 1 mg / ml 0.14 AHMT 6 mg / ml 0.12 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza

The measured values showed that there was no interference with the reagents. Normally the absorbance values were from 0.25 to 0.8 whereas in the tables they were either at the lower limit of the range (Table 12) or significantly lower (Table 13). Table 14 Interference of the POM with the reacted LDH reagent POM Medium POM concentration pitch - 0 0.00156 - Triton 0.01% 0.00133 0 - P / s ^a) 0.0016 Mo Pho 0.5 mM 0.00053 Mo Pho 1 mm 0.00041 Mo Pho 4mM 0.00008 Wo-Pho 0.5 mM 0.00123 Wo-Pho 1 mm 0.00115 Wo-Pho 4mM 0.0006 AHMT 80 μM 0.00127 AHMT 0.8mM 0.00122 AHMT 4.9 mM 0,002 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza Table 15: Interference of the POM with the unreacted LDH reagent POM Medium POM concentration pitch - 0 0.00187 - Triton 0.01% 0.00182 0 - P / S ^a) 0.00176 Mo Pho 0.5 mM 0.0011 Mo Pho 1 mm 0.00003 Mo Pho 4mM -0.00012 Wo-Pho 0.5 mM 0.00135 Wo-Pho 1 mm 0.00053 Wo-Pho 4mM 0.00019 AHMT 80 μM 0,002 AHMT 0.8mM 0,002 AHMT 4.9 mM 0.00123 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza

Normally, the slope of dead cells was 0.2. Since orders of magnitude lower values for the slope were obtained, there were no interferences with LDH.

The investigations underlined that the POMs did not show any adverse interactions with respect to the detection reactions, so that the results obtained in the further investigations were valid.

Example 4

The effect of ammonium heptamolybdate tetrahydrate (AHMT) on eukaryotes, determination of limit concentrations and mycoplasma decontamination

Ammonium heptamolybdate tetrahydrate (AHMT) was dissolved in MilliQ. For subsequent eukaryotic examinations, the required concentrations were adjusted by addition of DMEM medium including 10% FCS (fetal calf serum, 4 mM L-glutamate and 1 mM sodium pyruvate.) The cells were seeded on 96-well plates and incubated for 24 h in The cells were then incubated for 24 hours and 48 hours with the DMEM medium and DMEM with at least three different concentrations of AHMT as negative control and incubation with 0.01% Triton-X as positive control.

While all cells were metabolically active and alive in the negative control, all cells died in the positive control.

To study the influence of the media on cell proliferation and cell metabolism of the different cell lines, a WST-8 assay was performed at 24 hours and 48 hours. In this assay, metabolically active cells reduced the water-soluble WST-8 to the orange WST-8 formazan. This was used as a measure of cellular metabolism. To analyze the influence of the media on cytotoxicity, an LDH assay was performed in parallel with the WST-8 assay. In necrotic cells loss of membrane integrity led to the release of LDH. The water-insoluble INT-formazan (INT = 2- (4-iodophenyl) -3- (4-nitrophenyl) -5-phenyl-2H-tetrazolium chloride) resulting from the assay served as a measure of the released LDH.

To study the influence of AHMT, six different concentrations were initially selected to estimate the possible concentrations that could be used. The cell lines used were RAW 264.7 cells (macrophages), MDCK (NBL2) cells (epithelial cells) and NIH 3T3 cells (fibroblasts).

The cell cultures were assayed for the presence of mycoplasma using the Myco-Alert® Mycoplasma kit. To this was added 50 μl of Myco-Alert® reagent to each sample, after which the samples were incubated for 5 minutes. The measured values obtained were designated S. Next, 50 μl of Myco-Alert® substrate was added to each sample, after which the samples were incubated for 10 minutes. The measured values obtained were designated R. Thereafter, the ratio of S / R was formed. If the ratio was <0.9, no mycoplasmas are present. Values between 0.9 and 1.2 were borderline and the cultures in question had to be quarantined. Values> 1.2 showed mycoplasma contamination.

RAW 264.7 cells

An exemplary measurement (n = 2) was made with WST-8 assays to study the influence of AHMT on RAW 264.7 cells after 24 hours. The mean values and the standard deviations n of the absorptions were formed from in each case 4 replicates.

Furthermore, an exemplary measurement (n = 3) was performed with LDH assays to investigate the cytotoxic effect of AHMT RAW 264.7 cells after 24 hours and 48 hours. The mean values and the standard deviation n of the slope were formed from in each case 4 replicates.

The results of the WST-8 and LDH assays on RAW 264.7 cells are shown in Tables 16 and 17. TABLE 16 Influence of AHMT on Cell Metabolism and Cell Proliferation of RAW 264.7 Cells After 24 Hours - WST-8 Assay Medium AHMT concentration Absorption after 24 hours 0 0.4 P / S ^a) 0.414 Triton-X 0.01% 0 positive control 0.08 mM 0.457 0.4 mM 0.185 0.8mM 0,021 1.6 mM 0 2.4 mM 0 4.9 mM 0 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit-Lonza Table 17: Cytotoxic effect of AHMT on cellular metabolism and cell proliferation of RAW 264.7 cells after 24 hours and 48 hours by LDH assay Medium AHMT concentration Gradient after 24 hours Gradient after 48 hours 0 0.1 0.364 P / S ^a) .1007 0,375 Triton-X 0.01% Positive Control 0.321 0.316 0.08 mM 0.08 0.35 0.4 mM 0,071 0.3 0.8mM 0.166 0.35 1.6 mM 0.285 0.308 2.4 mM 0,271 0.303 4.9 mM 0.3 0.3 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit -Lonza

RAW 264.7 cells showed a reduction in their metabolic activity at 24 hours in the range of 0.08 mM to 0.8 mM AHMT. This reduction increases with increasing AHMT concentration, so that at a concentration of 2.4 mM AHMT comparable values were achieved as in the positive control with Triton-X. Parallel to this, a necrotic reaction of the RAW 264.7 cells to the AHTM was evident in the LDH test from a concentration of 0.8 mM.

This meant that the limiting concentration under the given conditions had to be <0.8 mM.

MDCK (NBL2) cells

As another cell line, epithelial cells of a dog (mardin darby canine kidney, MDCK (NBL2) cells) were used.

In Table 18, the exemplary evaluations of WST-8 assays for cell metabolism and cell proliferation of MDCK (NBL2) cells at 24 hours and 48 hours and Table 19 are the exemplary evaluations of LDH assays for the cytotoxic influence of AHMT on MDCK (NBL2) cells after 24 hours and 48 hours.

The mean values and the standard deviations n of the absorbances of the exemplary measurements (n = 3) were formed in each case from 4 replicates (Table 18).

The mean values and the standard deviations n of the slopes of the exemplary measurements (n = 3) were formed in each case from 4 replicates (Table 19). Table 18: The impact of AHMT on film metabolism on cell proliferation of MDCK (NBL2) cells after 24 hours and 48 hours using WST-8 assays Medium AHMT concentration Absorption after 24 hours Absorption after 48 hours 0 0.4 0.49 P / S ^a) 0.416 0.45 Triton-X 0.01% Positive Control 0.016 0.02 0.08 mM 0.439 0.514 0.4 mM 0.403 0.428 0.8mM 0.2 0,128 1.6 mM 0.1 0.028 2.4 mM 0.03 0,002 4.9 mM near 0 0,002 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza

However, the MDCK (NBL2) cells showed a concentration-dependent reduction in their metabolic activity in the range of 0.08 mM to 0.8 mM AHMT. Under the given conditions, the limit concentration was 0.4 mM. Table 19: Cytotoxic effect of AHMT on cell metabolism and cell proliferation of MDCK (NBL2) cells after 24 hours and 48 hours by LDH assays Medium AHMT concentration Gradient after 24 hours Gradient after 48 hours 0 0,025 0,045 P / S ^a) 0,025 0.04 Triton-X 0.01% Positive Control 0.207 0.25 0.08 mM 0,025 0.047 0.4 mM 0.03 0.08 0.8mM 0.05 0.085 1.6 mM 0,035 0.06 2.4 mM 0.027 0.0275 4.9 mM 0.20 0.06 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza

Despite the concentration-dependent reduction in metabolic activity, no necrotic effect was seen on the cells at concentrations of 0.08 mM to 4.9 mM.

Mouse fibroblasts (NIH-3T3, Swiss Albino Cells)

In Table 20, the exemplary evaluations of WST-8 assays for cell metabolism and cell proliferation of MDCK (NBL2) cells at 24 hours and in Table 21 are the exemplary evaluations of LDH assays for the cytotoxic effect of AHMT on NIH -3T3 cells shown after 24 hours.

The mean values and the standard deviations n of the absorbances of the exemplary measurements (n = 3) were formed in each case from 4 replicates (Table 20).

The mean values and the standard deviations n of the slopes of the exemplary measurements (n = 3) were formed in each case from 4 replicates (Table 21). Table 20: The influence of AHMT on cell metabolism and cell proliferation of NIH-3T3 cells after 24 hours - WST-8 assay Medium AHMT concentration Absorption after 24 hours 0 0.2 P / S ^a) 0.211 Triton-X 0.01% Positive Control 0,008 0.08 mM 0.211 0.4 mM 0.173 0.8mM 0.167 1.6 mM 0,035 2.4 mM 0,029 4.9 mM 0,006 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza Table 21: Cytotoxic effect of AHMT on cell metabolism and cell proliferation of NIH-3T3 cells after 24 hours by LDH assay Medium AHMT concentration Gradient after 24 hours 0 0,018 P / S ^a) 0,018 Triton-X 0.01% Positive Control 0,068 0.08 mM 0.016 0.4 mM 0,018 0.8mM 0.02 1.6 mM 0,018 2.4 mM 0.046 4.9 mM 0.032 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza

The NIH-3T3 cells showed a concentration-dependent reduction in their metabolic activity after 24 hours from an AHMT concentration of 0.8 mM. Under the given conditions, the limiting concentration was therefore 0.8 mM AHMT.

AHMT showed a non-significant necrotic influence on the NIH-3T3 cells from a concentration of 2.4 mM. In another measurement, however, no necrotic reaction to AHMT could be observed.

Eukaryotic cultures of RAW 264.7 cells (macrophages), MDCK (NBL2) cells (epithelial cells), and NIH 3T3 cells (fibroblasts) were prepared as described above. The cultures were buffered with Dulbecco's Phosphate Buffered Saline (PBS) powder. Subsequently, the cultures were incubated for 48 hours. After a holding time of 5 days, the supernatant media were separated from the cells. The separated media were assayed for mycoplasma-free cultures using Myco-Alert® assays and the Polymerase Chain Reaction (PCR) with GenePurge Direct® for DNA / RNA Releasing Agent. In all cases, the mycoplasma evidence was negative. The cells were mixed with fresh medium for further use.

• - MDCK (NBL2)-Zellen: 0,4 mM,
• - RAW 264.7-Zellen: 0,7 mM und nicht
• - NIH-3T3-Zellen: 0,8 mM.

Part of the cultures were infected with mycoplasma hominis. Subsequently, the infected cultures were added with AHTM in the following limit concentrations:

• MDCK (NBL2) cells: 0.4 mM,
• - RAW 264.7 cells: 0.7 mM and not
• NIH 3T3 cells: 0.8 mM.

The presence of mycoplasma hominis in the cultures was verified using the above test methods. Subsequently, the infected cultures were incubated for 4 days. After a Life of 5 days, the supernatant media were separated from the cells. The separated media were assayed for mycoplasma-free cultures using Myco-Alert® assays and the Polymerase Chain Reaction (PCR) with GenePurge Direct® for DNA / RNA Releasing Agent. Mycoplasmas could no longer be detected. The cells were added with fresh media. The refreshed cultures were incubated and it was found that the eukaryotes were still capable of proliferation.

In order to rule out that the benefits and effects found are partly or entirely attributable to daylight, the tests were carried out in the dark and in daylight. No differences were found, which is why the advantages and effects are due solely to the polyoxometalates.

Example 5

The effects of Mo-Pho and Wolframatophosphoric Hydrate (Wo-Pho) on eukaryotes, determination of limit concentrations, and mycoplasma decontamination

Wolframatophosphoric hydrate (Wo-Pho) and molybdophosphoric hydrate (Mo-Pho) were dissolved in MilliQ. For the subsequent studies of the eukaryotes, the required concentrations were adjusted by addition of DMEM medium, including 10% FCS (fetal calf serum), 4 mM L-glutamate and 1 mM sodium pyruvate. The cells were seeded on 96-well microtiter plates and cultured for 24 hours in the DMEM medium. This was followed by a 24-hour incubation of the cells with the DMEM medium and DMEM with at least three different POM concentrations as a negative control and incubation with 0.01% Triton-X as a positive control.

In the following two tables, the results of each measurement (n = 1) were shown, with the mean and standard deviations n of the absorptions and the slopes from each of 3 replicates being formed. The influence of Mo-Pho and Wo-Pho on cell proliferation and cell metabolism of MDCK (NBL2) cells after 24 hours - WST-8 assays POM concentration Absorption after 24 hours medium 0 0,383 P / S ^a) 0 0.391 Triton-X 0.01% 0 0,008 Mo Pho 0.05mM 0,129 Mo Pho 0.5 mM 0.191 Mo Pho 1 mm 0,075 Wo-Pho 0.01 mM 0.362 Wo-Pho 0.1 mM 0.183 Wo-Pho 0.5 mM 0.2 a) Ratio of 2 measurements R and S of the MycoAlert ™ Mycoplasma Detection Kit - Lonza Table 23: The cytotoxic effect of Mo-Pho and Wo-Pho on MDCK (NBL2) cells after 24 hours - LDH assays POM concentration Gradient after 24 hours medium 0 0.048 P / S ^a) 0 0.048 Triton-X 0.01% 0 0.214 Mo Pho 0.05mM 0.048 Mo Pho 0.5 mM 0.048 Mo Pho 1 mm 0,375 Wo-Pho 0.01 mM 0.05 Wo-Pho 0.1 mM 0.05 Wo-Pho 0.5 mM 0.93

Mo-Pho showed no necrotic influence on the MDCK (NBL2) cells at any concentration. Cell proliferation appeared slightly inhibited at a concentration of 1mM. Under the given conditions, the limiting concentration of Mo-Pho could be chosen between 0.5 mM and 1 mM.

In contrast, Wo-Pho showed a necrotic effect on the MDCK (NBL2) cells from a concentration of 0.5 mM. The frontier confinement Wo-Pho should therefore be <0.5 mM.

Eukaryotic cultures of MDCK (NBL2) cells were prepared as described above. The cultures were buffered with Dulbecco's Phosphate Buffered Saline (PBS) powder. The cultures were then incubated for 24 hours, and after 5 days' storage, the supernatant media were separated from the cells and resuspended with GenePurge Direct® for DNA / using the Myco-Alert® Assays and Polymerase Chain Reaction (PCR). RNA Releasing Agent tested whether the eukaryotic cultures were mycoplasma-free. In all cases, the mycoplasma evidence was negative. The cells were mixed with fresh medium for further use.

• - Mo-Pho: 0,7 mM und
• - Wo-Pho: 0,3 mM.

Part of the MDCK (NBL2) cultures were infected with mycoplasma hominis. Subsequently, the infected cultures Mo-Pho or Wo-Pho were added in the following limit concentrations:

• - Mo-Pho: 0.7 mM and
• - Wo-Pho: 0.3 mM.

The infected cultures were incubated for 24 hours. After a holding time of 5 days, the supernatant media were separated from the cells. The separated media were assayed for mycoplasma-free cultures using Myco-Alert® assays and the Polymerase Chain Reaction (PCR) with GenePurge Direct® for DNA / RNA Releasing Agent. Mycoplasmas could no longer be detected. The cells were added with fresh media. The refreshed cultures were incubated and it was found that the eukaryotes were still capable of proliferation.

In order to rule out that the benefits and effects found are partly or entirely attributable to daylight, the tests were carried out in the dark and in daylight. No differences were found, which is why the advantages and effects are due solely to the polyoxometalates.

Example 7

The influence of tungstosilicic acid (WCS) on cultures of A549 cells

To determine whether WCS was able to eliminate mycoplasmas in cell cultures, two different experiments were performed.

WCS were solved in MilliQ. For the subsequent studies of the eukaryotes, the required concentrations were adjusted by addition of DMEM medium, including 10% FCS (fetal calf serum), 4 mM L-glutamate and 1 mM sodium pyruvate. The cells were seeded on 96-well microtiter plates and cultured for 24 hours in the DMEM medium.

First, (i) the A549 cells with and without mycoplasma hominis were incubated with high concentrations of WCS for 30 minutes. Thereafter, (ii) the A549 cells with and without mycoplasma hominis were incubated with low concentrations of POM for 4 days. Residual mycoplasmas were detected in the infected cell cultures in the cells above the cells using the MycoAlert ™ Mycoplasma Detection Kit - Lonza. In this regard, it was necessary to collect the supernatant fluids before and after incubation to demonstrate that WKS had now eliminated the mycoplasmas in the infected cultures.

Experiment (i) - short incubation period - high POM concentrations

For the short incubation period of 30 minutes with high concentrations of WCS a confluence of 80% was necessary. The cultures with the adherent cells were buffered with dipotassium hydrogen phosphate and incubated for 30 minutes with 10 mM, 5 mM, 2.5 mM and 0 mM of WKS. Cell viability was determined by light microscopy images during incubation. After incubation, the media were discarded. The cells were washed twice with PBS and new media were added. The cell viability was determined several times during 5 days to determine the recovery of the cells. Afterwards, the detection methods for mycoplasmas could be applied.

Experiment (ii) - long incubation period - low POM concentrations

Also for the long incubation period of 4 days with low concentrations of WCS a confluence of 80% was necessary. Cell cultures were cultured in media containing WKS and dipotassium hydrogen phosphate for 4 days. WKS at concentrations of 1.25 mM, 0.63 mM, 0.31 mM, 0.16 mM and 0 mM were used. The cell viability was optically determined several times during 4 days. After the 4-day incubation with the POM, the media was discarded and new media containing WCS and dipotassium hydrogen phosphate were added to the cells. The cells were grown for 5 days before mycoplasma detection with the MycoAlert ™ Mycoplasma Detection Kit - Lonza and the polymerase chain reaction (PCR) with GenePurge Direct® for DNA / RNA Releasing Agent.

Results

The results of experiments (i) and (ii) showed that short incubation times of 30 minutes with high concentrations of WCS were more cell damaging than long incubation times of 24 hours with low concentrations of WCS. For these reasons, a pure A549 cell line and an A549 cell line infected with mycoplasma hominis were spiked with WCS at a concentration of 10 mM, buffered with dipotassium hydrogen phosphate, for 30 minutes each time. Cell growth was not disturbed and the pure A549 cell line was still mycoplasma negative, which was expected. However, the infected A549 cell line was still mycoplasma positive. This demonstrated that WKS was unable to eliminate the mycoplasmas, even at high WKS concentrations, with short incubation times.

Thus, the pure A549 cell lines and the A549 cell lines infected with mycoplasma hominis were spiked and incubated for 4 days with low concentrations of WCS (0.63 mM, 31 mM and 0.16 mM). An addition of dipotassium hydrogen phosphate was not necessary. After the 4-day incubation, the pure A549 cell lines were expected to be mycoplasma-free as evidenced by Myco-Alert® assays and the polymerase chain reaction (PCR) with GenePurge Direct® for DNA / RNA releasing agent assays. In the infected A549 cell lines, a strong reduction of Mycoplasmas occurred, which was strongest at the WKS concentration of 0.63 mM. At most, this culture still contained traces of dead mycoplasma DNA. In addition, the mycoplasma-free A549 cell lines were still capable of proliferation.

In order to rule out that the benefits and effects found are partly or entirely attributable to daylight, the tests were carried out in the dark and in daylight. No differences were found, which is why the advantages and effects are due solely to the polyoxometalates.

The relevant measurement results are summarized in Table 24. Table 24: Results of mycoplasma detection after treatment of infected A549 cell cultures with WCS after 4-day incubations A549 cultures / WKS concentration Measurement R Measurement S Ratio S / R Before the trials 2385 2023 0.85 Control with 0 mM 2934 1831 0.62 With mycoplasma hominis infected before the experiments 2819 53620 19 Infected with mycoplasma hominis 0 mM 2732 54280 19.9 With mycoplasma hominis infected 0.16 mM 1698 1748 1.03 With mycoplasma hominis infected 0.31 mM 1523 1327 0.87 Infected with mycoplasma hominis 0.63 mM 1164 1037 0.89 positive control 2220 159500 71.8 negative control 3726 859.7 0.23

• < 0,9 - negativ für Mykoplasmen
• 0,9 bis 1,2 - Grenzfälle, Zellkulturen müssen in Quarantäne
• >1,2 - Mykoplasmenkontamination

Rating of S / R:

• <0.9 - negative for mycoplasma
• 0.9 to 1.2 - borderline cases, cell cultures must be quarantined
• > 1.2 - mycoplasma contamination

Example 8

The antibacterial effect of POM on P. aeruginosa (Gram-negative) and S. aureus (Gram-negative)

To determine whether AHMT, Wo-Pho or WKS at concentrations of 0.31 mM, 0.63 mM, 1.25 mM, 2.5 mM and 5 mM, the bacteria after an incubation time of 0, 10 minutes, 20 minutes, 30 minutes and 24 hours, corresponding MIC (Minimal Inhibitory Concentration) assays were performed.

It was found that none of the concentrations were able to kill S. aureus after 0 hours of incubation. After 24 hours incubation of S. aureus in a DMEM medium with Wo-Pho showed that Wo-Pho at a concentration of 2.5 mM after a 24 hour incubation period could kill the bacteria. In an EMEM medium, only 1.25 mM was necessary.

The experiments were repeated with AHMT and it was found that S. aureus could be killed after a 24-hour incubation at a concentration of 5 mM AHMT. In contrast, WKS required a concentration of 2.5 mmol / l.

AHMT, Wo-Pho, or WKS at concentrations of 0.31 mM, 0.63 mM, 1.25 mM, 2.5 mM, and 5 mM were unable to kill P. aeruginosa after an 0 hour incubation. In contrast, Wo-Pho or WKS at a concentration of 5 mM were able to kill P. aeruginosa in an EMEM medium after 24 hours of incubation.

Further, short-term incubations were performed to test the effect of WKS and WoPho after 10 minutes, 20 minutes and 30 minutes incubation. The highest concentration of POM was 10 mM in the medium buffered with dipotassium hydrogen phosphate. Previously, it was ensured that dipotassium hydrogen phosphate had no effect on the bacteria. The test results are summarized in Table 25 to. Table 25: The antibacterial effect of Wo-Pho and WKS on P. aeruginosa (gram-negative) and S. aureus (gram-positive) Wo-Pho Concentration mM Incubation for 10 minutes S. aureus necrosis? P. aeruginosa necrosis? " 0.31 " No No " 0.63 " " " " 1.25 " " " " 2.5 " " " " 5 " " " " 7.5 " " " " 10 " " " Wo-Pho Incubation for 20 minutes S. aureus necrosis? P. aeruginosa necrosis? " 0.31 " No No " 0.63 " " " " 1.25 " " " " 2.5 " " " " 5 " No, just inhibition No, just inhibition " 7.5 " No, just inhibition No, just inhibition " 10 " No, just inhibition No, just inhibition Wo-Pho Incubation for 30 minutes S. aureus necrosis? P. aeruginosa necrosis? " 0.31 " No No " 0.63 " No No " 1.25 " " " " 2.5 " " " " 5 " " " " 7.5 " " No, just inhibition " 10 " " No, just inhibition WKS Incubation for 10 minutes S. aureus necrosis? P. aeruginosa necrosis? " 0.31 " No No " 0.63 " " " " 1.25 " " " " 2.5 " " " " 5 " " " " 7.5 " " " " 10 " " Yes WKS Incubation for 20 minutes S. aureus necrosis? P. aeruginosa necrosis? " 0.31 " No No " 0.63 " " " " 1.25 " " " " 2.5 " " " " 5 " " No, just inhibition " 7.5 " " No, just inhibition " 10 " " Yes WKS Incubation for 30 minutes S. aureus necrosis? P. aeruginosa necrosis? " 0.31 " No No " 0.63 " " " " 1.25 " " " " 2.5 " " " " 5 " " No, just inhibition " 7.5 " " No, just inhibition " 10 " " Yes

While WKS and Wo-Pho have an inhibitory and cytotoxic effect on P. aeruginosa at comparatively high concentrations, both POMs are neither cytotoxic nor inhibiting on S. aureus. The bactericidal effect of POM is therefore at best weak.

However, this drawback becomes an advantage because POM's weak to lacking bactericidal activity leaves a large margin for POM concentrations to eliminate mycoplasma in infected bacterial cultures.

In order to rule out that the benefits and effects found are partly or entirely attributable to daylight, the tests were carried out in the dark and in daylight. No differences were found, which is why the advantages and effects are due solely to the polyoxometalates.

Example 9

Protective medical devices for the prophylaxis and / or topical control of mollicuten infections

Medical devices for topically controlling mollicin infections contained polyoxometalates at a concentration below the limiting concentration. For the skin were plasters, dispensers for ointments, creams and lotions, for the nose dosers for nose drops, sprays and ointments, for the oral mucosa bottles for gargle solutions and lozenges, for respiratory inhalation systems for aerosols and powders, for the intestinal enemas and Enema, for the conjunctivae and corneas of eyes Dropping bottles and dispensers for ointments and for the joints syringes used with injection solutions.

The medical devices were excellently suited for the topical control of mycoplasma infections in humans and animals.

QUOTES INCLUDE IN THE DESCRIPTION

This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

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Claims (28)

Extracellular and / or intracellular use of at least one polyoxometalate for the prophylaxis and / or aftercare of mollicut infections and / or mollicut contamination, for the temporary or permanent killing and / or decontamination of mollicut contaminants, for the temporary or permanent inhibition of the proliferation of Mollicutes and / or for the temporary or permanent adjustment of a constant concentration of mollicuts from and in virus cultures, cultures of unicellular and multicellular eukaryotes, and of and in microorganism populations, wherein - the at least one polyoxometalate is used at most in a limit concentration determined in each case in at least one test culture in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure, in which - The proliferation of Mollicut be inhibited or killed, but in the the at least one polyoxometalate after an incubation time of a given eukaryotic test culture, a given virus test culture or at least one test culture in the dark originating from a given microorganism population and / or under exposure to electromagnetic radiation and / or under atmospheric pressure or overpressure from 0 hours to permanent and after at least a Mollicutendetektion after a lifetime in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure of 0 hours to permanent proliferation of the respective eukaryotes, the respective host cells of the virus or the microorganisms concerned not yet inhibited. Extracellular and / or intracellular use of a polyoxometalate Claim 1 , characterized in that the microorganism populations contain at least two members selected from the group consisting of eukaryotes, bacteria, microalgae, protozoa, bacteriophages and viruses. Extracellular and / or intracellular use of at least one polyoxometalate Claim 1 or 2 , characterized in that - the incubation time and / or the lifetime 10 minutes to 100 days, - the incubation temperature and / or the stand temperature 0 ° C to 100 ° C and / or - incubation pressure and / or the 1.0 bar pressure to 50 is bar and - the electromagnetic radiation of lights, lamps and / or lasers is continuously or with at least one interruption radiated far infrared, near infrared (NIR), visible light and / or UV radiation. Extracellular and / or intracellular use of at least one polyoxometalate according to one of Claims 1 to 3 , characterized in that the cultures of - unicellular and / or multicellular eukaryotes, - viruses and - microorganism populations of drugs, cell cultures, 3D cell cultures, cell biological nutrient systems, cell culture media, tissue cultures, 3D tissue cultures, organs, organ transplants, artificially produced organs, On-chip organs, auxiliary media, biochemistry laboratories, molecular biology, genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology, and medicine, as well as articles, prints, furniture, clothing, equipment, apparatus, and laboratory personnel for and in these laboratories. Extracellular and / or intracellular use of at least one polyoxometalate according to one of Claims 1 to 4 , characterized in that the mollicutes in the infected cultures are mycoplasmas. Extracellular and / or intracellular use of at least one polyoxometalate according to one of Claims 1 to 5 , characterized in that the limiting concentration of the at least one polyoxometalate at an incubation time of an infected test culture of 30 minutes in the dark with a Mollicutendetektion after another 5 days of standing in the dark <15 mM and the limiting concentration of at least one Polyoxometallats for an incubation period of 4 days in the dark with a Mollicutendetektion after another 5 days of standing in the dark <1.0 mM. Extracellular and / or intracellular use of at least one polyoxometalate according to one of Claims 1 to 6 characterized in that the at least one polyoxometalate is selected from the group consisting of unmodified, oxygen-substituted, functionalized, coated, grafted, aggregated, agglomerated and supported Polyoxometallatmolekülen a largest molecular diameter ≤2 nm and Polyoxometallatnanopartikeln and Polyoxometallatmikropartikeln an average particle size of 1 nm to <1000 microns, is selected. Extracellular and / or intracellular use of at least one polyoxometalate according to one of Claims 1 to 7 , characterized in that the at least one polyoxometalate in at least one polyoxometalate preparation - in the form of an acid, a salt, a hydrate, a soft oxometalate (SOMS), a coating and / or a polyoxometalate nanocomposite, and / or - in molecular dispersions of dissolved, buffered, suspended, lipophilized and / or fluidized with organic groups and / or in and / or as dispersing, partially dissolving, non-dissolving or completely dissolving, compact and / or porous particles, coatings, coatings, release materials Sponges, wafers, pieces of foil, pieces of textile, pieces of tissue, cellulosic fibers, cellulose microparticles, cellulosic nanoparticles, nanosomes, microsomes, liposomes, tablets, glass beads, sprayers, nebulisers and / or as a coating which renders the surface hard and smooth and / or diffusion-closed, and / or - in aerosols, mists, hydrogels, in Mice in vesicles, in microcapsules, in microspheres, in gels, in creams, lotions, ointments and / or foams and / or in conjunction with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary media for cell biology and / or - in the form of its starting materials, which spontaneously combine to form the at least one polyoxometalate. Extracellular and / or intracellular use of at least one polyoxometalate according to one of Claims 1 to 8th , characterized in that the at least one polyoxometalate is selected from the group consisting of: - ammonium heptamolybdate tetrahydrate {(NH ₄ ) ₆ Mo ₇ O ₂₄ ] · 4H ₂ O, CAS No. 13106-76-8 (anhydrous), CAS No. 12054-85-2 (tetrahydrate), AHMT}, - tungstophosphoric hydrate {H ₃ [P (W ₃ O ₁₀ ) ₄ ] xH ₂ O, CAS-No. 1343-93-7 (anhydrous), CAS no. 12067-99-1 (hydrate), Wo-Pho}, - molybdophosphoric acid hydrate, {H ₃ P (Mo ₃ O ₁₀ ) 4] xH 2 O, CAS No .: 12026-57-2 (anhydrous), CAS No. 51429-74-4 (hydrate), Mo-Pho} and - tungstosilicic acid {H ₄ [Si (W ₃ O ₁₀ ) ₄ ] .xH ₂ O, CAS-No. 12027-43-9, CAS no. WCS} is selected. Extracellular and / or intracellular use of at least one polyoxometalate according to one of Claims 1 to 9 , characterized in that the eukaryotes, viruses and microorganism populations of the infected cultures are from vertebrates, deuterostomes, proterostomes, tissue animals, multicellular organisms, unicellular organisms, plants, algae and / or arthropods. Extracellular and / or intracellular use of at least one polyoxometalate Claim 8 , characterized in that the eukaryotes, viruses and microorganism populations of the infected cultures of humans, mammals, birds, reptiles, amphibians, fish, bony fish, cartilaginous, non-jawed, echinoderms, annelids, centipedes, crustaceans, arachnids, insects, molluscs, tubular worms , Flat worms, hollow animals, sponges, protists and algae. Cultures of microorganism populations which contain at least one polyoxometalate and contain at least one polyoxometalate, the cultures of medicaments, cellulobiological nutrient systems, cell biological nutrient media, tissue cultures, 3D tissue cultures, organs, organ transplants, artificially produced organs "On-chip organs", auxiliary media, biochemistry laboratories, molecular biology, genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology and medicine, as well as articles, furniture, prints, clothing, implements and apparatus for and in these laboratories; the at least one polyoxometalate can be used at most in a limit concentration determined in each case in at least one test culture in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure, in the - d or at least one polyoxometalate after incubation of a given eukaryotic test culture, virus test culture, or at least one test culture originating from a given microorganism population in the dark and / or under irradiation with electromagnetic radiation; under Atmospheric pressure or overpressure from 0 hours to permanent and after at least one Mollicutendetektion after a lifetime in the dark and / or under irradiation with electromagnetic radiation and / or atmospheric pressure or overpressure of 0 hours to permanent proliferation of the respective eukaryotes, the respective host cells Viruses or the microorganisms in question is not yet inhibited. Mollicut-free proliferation-competent cultures containing at least one polyoxometalate Claim 10 , characterized in that the Mollicutes are mycoplasmas. Mollicut-free proliferation-competent cultures containing at least one polyoxometalate Claim 12 or 13 , characterized in that the unicellular and / or multicellular eukaryotes, the host cells of the virus cultures and the microorganism populations of the cultures and the test cultures of vertebrates, deuterostomes, proterostomes, tissue animals, multicellular organisms, unicellulars, plants, algae and / or arthropods, the expression "Derived from" in the sense that the eukaryotes are derived from the living beings as such, ie cells of these living beings, whereas the viruses and the microorganism populations colonize said living beings and are taken in the form of samples. From proliferating Mollicuten free, containing at least one polyoxometalate cultures according to one of Claims 12 to 14 characterized in that the unicellular and / or multicellular eukaryotes, the host cells of the virus cultures and the microorganism populations of the cultures and the test cultures of humans, mammals, birds, reptiles, amphibians, fish, bony fish, cartilaginous, pine, echinoderm, annelids, centipedes, Crustaceans, arachnids, insects, molluscs, tubeworms, flatworms, cows, sponges, protists and algae, the term "derived from" meaning that the eukaryotes are derived from the animals as such, ie cells of these animals whereas the viruses and microorganism populations populate said animals and are sampled. Kits for the extracellular and / or intracellular use of at least one polyoxometalate according to one of Claims 1 to 11 , in each case (A) at least one apparatus for carrying out spectroscopic, microbiological and / or chemical measurements for the qualitative and / or quantitative detection of unicellular eukaryotes, multicellular eukaryotes, host cells of viruses, microorganism populations and mollicutes on at least one test sample, the at least one test culture infected with at least one type of mollicut containing at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes, at least one kind of host cells or at least one kind of microorganism populations is removable, (B) at least one device for evaluating and storing the test results measured on the at least one test sample; (C) free microbiological nutrient media, nutrient systems, auxiliary media, equipment and apparatus for producing at least one type of mollicutene for producing at least one of at least one Type of Mollicut free culture containing (C1) at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes, (C2) at least one kind of host cells for viruses or (C3) at least one kind of microorganism populations, as a null sample in which the eukaryotes, the viruses or the microorganism population of drugs, cell cultures, 3D cell cultures, cell biological nutrient systems, cell biological nutrient media, tissue cultures, 3D tissue cultures, organs, organ transplants, artificially produced organs, on-chip organs, auxiliary media and smears of Laboratories for cell biological nutrient systems, cell culture media, tissue cultures, 3D tissue cultures, organs, organ transplants, man-made organs, on-chip organs, auxiliary media, biochemistry laboratories, virology, microbiology, pharmacology, toxicology and medicine, and articles, Furniture, prints, clothes, ger (D) cell biological nutrient media, cell biological nutrient systems, auxiliary media, equipment and apparatus for preparing at least one standard cell biological preparation with a known dose (D1) of at least one kind of unicellular eukaryotes and / or at least a kind of multicellular eukaryotes, (D2) at least one kind of host cells for viruses or (D3) at least one kind of microorganism populations infected with a precisely known dose of at least one kind of mollicutae, (E) at least one preparation of a precisely known dose at least a polyoxometalate and (F) at least one metering device for the at least one preparation of a precisely known dose of at least one polyoxometalate. Kits for the extracellular and / or intracellular use of at least one polyoxometalate after Claim 16 characterized in that said at least one polyoxometalate is selected from the group consisting of unaltered, oxygen-substituted, functionalized, coated, grafted, aggregated, agglomerated and supported polyoxometalate molecules of largest molecular diameter ≤2 nm and polyoxometalate nanoparticles and polyoxometalate microparticles of median particle size from 1 nm to <1000 μm, is selected. Kits for the extracellular and / or intracellular use of at least one polyoxometalate after Claim 16 or 17 , characterized in that the at least one polyoxometalate and / or the at least one POM preparation - in the form of an acid, a salt, a hydrate, a soft oxometalate (SOMS), a coating and / or a polyoxometalate nanocomposite, and / or - in molecularly dispersed, suspended, lipophilized and / or fluidized with organic groups form and / or - in and / or as dispersing, partially dissolving, non-dissolving or completely dissolving, compact and / or porous particles, coatings, coatings, Release materials, sponges, platelets, foil pieces, textile pieces, fabric pieces, cellulose fibers, cellulose microparticles, cellulosic nanoparticles, nanosomes, microsomes, liposomes, tablets, glass beads, sprayers, nebulizers and / or as a coating which renders the surface hard and smooth and / or diffusion-closed, and / or - in aerosols, mists, hydrogels, in micelles, in vesicles , in microcapsules, in microspheres, in gels, in creams, lotions, ointments and / or foams and / or in conjunction with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary media for cell biology and / or Form of its starting materials, which spontaneously combine to form the at least one polyoxometalate, is present. Kits for the extracellular and / or intracellular use of at least one polyoxometalate according to one of Claims 16 to 18 , characterized in that the at least one polyoxometalate selected from the group consisting of - ammonium heptamolybdate tetrahydrate {(NH ₄ ) ₆ Mo ₇ O ₂₄ ] · 4H ₂ O, CAS no. 13106-76-8 (anhydrous), CAS no. 12054-85-2 (tetrahydrate), AHMT}, - tungstophosphoric hydrate {H ₃ [P (W ₃ O ₁₀ ) ₄ ] .xH ₂ O, CAS-No. 1343-93-7 (anhydrous), CAS no. 12067-99-1 (hydrate), Wo-Pho}, - molybdophosphoric acid hydrate, {H ₃ P (Mo ₃ O ₁₀ ) 4] .xH ₂ O, CAS No: 1206-57-2 (anhydrous), CAS No. 51429-74-4 (hydrate), Mo-Pho} and tungstosilicic acid {H ₄ [Si (W ₃ O ₁₀ ) ₄ ] .xH ₂ O, CAS-No. 12027-43-9, CAS no. WCS} is selected. Kits after one of Claims 16 to 19 , characterized in that the Mollicutes are mycoplasmas. Kits after one of Claims 16 to 20 , characterized in that the kits are controlled by a data processing system, automated robots. Method (i) for decontaminating mollicutin-infected eukaryotic cultures, virus cultures and cultures of microorganism populations and (ii) for proliferation of the decontaminated, mollicutene-free cultures, characterized in that (G) at least one mollicut-free and / or at least one mollicutin-infected test culture (G1) at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes, (G2) at least one kind of host cells for viruses or (G3) at least one kind of microorganism populations the limiting concentration of at least one polyoxometalate for inhibiting the proliferation of the at least one kind of Eukaryotes, host cells for viruses or microorganism populations determines (H) at least one Mollicuteninfected culture (H1) of at least one kind of unicellular eukaryotes and / or at least one kind of multicellular eukaryotes, (H2) of at least one kind of host cells for r viruses or (H3) the at least one type of microorganism populations whose limit concentration of the at least one polyoxometalate for inhibiting proliferation has been determined after process step (G), admixed with the at least one polyoxometalate so that in the at least one mollicuteninfiziert culture at most the limiting concentration of at least one Polyoxometallats results, (I) the after process step (H) resulting at least one Mollicuteninfiziert culture during a incubation time in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure of 0 hours to permanent incubation and after a lifetime in Darkening and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure from 0 hours to permanently at least once determines whether there are still Mollicutes and, if this is not the case, (J) at least a proportion of min at least one culture (J1) of at least one type of unicellular eukaryote and / or at least one type of multicellular eukaryote, (J2) transmits at least one type of host cell for viruses or (J3) at least one type of microorganism populations to at least one mollicin-free nutrient medium; each propagating at least one species of mollicut-free eukaryotes, host cells and microorganism populations in the resulting at least second culture. Process (i) for the decontamination of mollicutin-infected eukaryotic cultures, virus cultures and cultures of microorganism populations and (ii) for the proliferation of the decontaminated, mollicutene-free cultures Claim 22 Characterized in that the eukaryotes, the virus or microorganism populations of drugs, cell cultures, 3D cell cultures, cell biological Nährsystemen, cell biological growth media, tissue cultures, 3D tissue cultures, organs, organ transplants, organs artificially produced, organs "on a chip" and / or auxiliary media, as well as laboratories for biochemistry, molecular biology, genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology and medicine, and articles, prints, furniture, clothing, implements, equipment and laboratory personnel for and in these laboratories and / or that the mollicut mycoplasmas are. Process (i) for the decontamination of mollicutin-infected eukaryotic cultures, virus cultures and cultures of microorganism populations and (ii) for the proliferation of the decontaminated, mollicutene-free cultures Claim 22 or 23 , characterized in that for this purpose at least one kit according to one of Claims 16 to 20 used. Process (i) for the decontamination of mollicutin infected eukaryotic cultures, virus cultures and cultures of microorganism populations and (ii) for the proliferation of the decontaminated, mollicin - free cultures according to any one of Claims 22 to 24 , characterized in that the method is carried out automatically using a robot kit. Anti-Mollicut infestation and / or protective articles, medical devices and fluids containing at least one polyoxometalate and / or at least one polyoxometalate preparation according to any one of Claims 17 to 19 , which or which - in the form of an acid, a salt, a hydrate, a soft oxometalate (SOMS), a coating and / or a polyoxometalate nanocomposite, and / or - in and / or on as dispersing, partially dissolving, non-dissolving or completely dissolving, compact and / or porous particles, coatings, coatings, release materials, sponges, platelets, foil pieces, textile pieces, fabric pieces, cellulose fibers, cellulose microparticles, cellulose nanoparticles, nanosomes, microsomes, liposomes, tablets, glass beads, sprayers, nebulisers and / or as a coating which renders the surface hard and smooth and / or diffusion-closed, and / or - in aerosols, mists, hydrogels, in micelles, in vesicles, in microcapsules, in microspheres, in gels, in creams, lotions, ointments and / or foaming and / or - in conjunction with functional materials, antibiotics, biocidal materials and / or nutrient media and / or auxiliary agents media for cell biology and / or - in the form of its starting materials, which spontaneously assemble into the at least one polyoxometalate, introduced onto and / or into the articles and / or into the fluids in a limit concentration determinable on eukaryotic test cultures, virus test cultures or test cultures of microorganism populations or are, where - in order to determine the respective limiting concentration, the at least one polyoxometalate and / or the at least one polyoxometalate preparation can be used at most in at least one test culture in the dark and / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure concentration, in which the at least one polyoxometalate and / or the at least one polyoxometalate preparation are incubated after incubation of a given eukaryotic test culture, virus test culture, or at least one test culture in the dark, and from a given microorganism population / or under irradiation with electromagnetic radiation and / or under atmospheric pressure or overpressure of 0 hours until permanently and after at least one Mollicutendetektion after a life in the dark and / or under irradiation with electromagnet ischemic radiation and / or under atmospheric pressure or overpressure of 0 hours until permanently the proliferation of the respective eukaryotes, the respective host cells of the virus or of the microorganisms in question is not yet inhibited. Anti-Mollicut infestation and / or protective articles and medical devices and fluids containing at least one polyoxometalate and / or at least one polyoxometalate preparation Claim 26 , characterized in that the particles, aerosols, mist, sprayers, nebulizers, hydrogels, gels, creams, lotions, ointments and / or foams for decontamination of the laboratory staff of Mollicutes are dosed without risk of damage to crops and that the medical devices dispenser for Ointments, creams and lotions, nasal drops, sprays and ointment dosers, gargles and lozenge bottles, aerosol and powder inhalation systems, enemas and enemas, eye dropper bottles and ophthalmic ophthalmic dispensers and syringes with injection solutions. Decontamination and sterilization of pharmaceuticals, cellulosic nutrient systems, cellulosic nutrient media and of biochemistry, molecular biology, genetic engineering, cell biology, microbiology, virology, pharmacology, toxicology and medicine laboratories and of articles, furniture, prints, clothing, equipment, and laboratory personnel and in these laboratories, characterized in that for this purpose at least one cleaning agent, the at least one polyoxometalate and / or at least one polyoxometalate preparation in a minimum dosage> the limiting concentration according to Claim 1 contains, used.

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