CN2646712Y - Quick test sheet paper for fungus and microzyme - Google Patents
Quick test sheet paper for fungus and microzyme Download PDFInfo
- Publication number
- CN2646712Y CN2646712Y CN 03274715 CN03274715U CN2646712Y CN 2646712 Y CN2646712 Y CN 2646712Y CN 03274715 CN03274715 CN 03274715 CN 03274715 U CN03274715 U CN 03274715U CN 2646712 Y CN2646712 Y CN 2646712Y
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- China
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- utility
- model
- cultivating
- microzyme
- epiphragma
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- Expired - Fee Related
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model relates to a quick testing slip for the mildew and the microzyme and belongs to the microbiology testing art domain. The utility model consists of an upper velum which is provided with sop gel powder with enzyme visualization reagent in the bottom surface and a cultivating slice which absorbs the cultivating liquid. The utility model has a better testing effect compared with the prior art; the utility model is convenient to be operated and leaves out a great of assisting works such as preparing the substrate and the disinfection and cleaning of the cultivating device; the utility model saves the cultivating time of the mildew and the microzyme and can sample and detect in any time; and the utility model will not be limited by the environment, the experimental device and the technical staff.
Description
(1) technical field
Technical field under the utility model is a microbiological Test, relates to mould and saccharomycetic a kind of quick test articles for use.
(2) background technology
The classic method of mould and saccharomycete check has: fall flat band method.Method is that the starting material of the preparation nutrient culture media that will be taken by weighing are put into water and boiled moltenly, treats that nutrient culture media is cooled to about 45 ℃, inserts sample liquid in the double dish, pour nutrient culture media into, keep flat and be cooled to flat board, thermophilic is observed, is counted after cultivating a week down, record mould and saccharomycetic growing state.
The operated in accordance with conventional methods complex steps will be done a large amount of auxiliary works before and after mould and saccharomycete are cultivated, count, cultivate vessel etc. as preparation nutrient culture media, sterilization, cleaning, and is very high to the requirement of experimental implementation technology.Temperature control requirement is rigorous, and incubation time is long, and the cycle reached about 1 week.
(3) summary of the invention
1, technical matters to be solved by this invention:
For solve described in the background technology to the existing problem of mould and saccharomycete check aspect, it is short to design a kind of easy and simple to handle, proving time, the disposable check scraps of paper are used for mould and saccharomycete being carried out quick test, the purpose of this utility model that Here it is.
2, the technical solution used in the present invention:
The utility model is made up of last epiphragma and cultivation sheet.Last epiphragma coated on bottom side cloth contain the water absorbent gel powder of enzyme developer, be waterproof and breathable, the transparent membrane that has graticule line, the tape square line is to observe counting for convenience.Cultivating sheet is the scraps of paper that adsorbed liquid medium, and it is bonded at below the epiphragma.In order to carry epiphragma and to cultivate sheet, forms an excellent environment that helps mould and saccharomycete growth, prevent the moisture turnover, be preferably in the egative film that one deck waterproof and breathable is arranged below epiphragma and the cultivation sheet, egative film is preferably made by synthetic material.
With the utility model mould and saccharomycetic check are realized by following process.
1) will check scraps of paper level to be placed on the table top, open epiphragma, draw sample stoste or dilution 1mL, and evenly be added to and cultivate on the sheet, will go up epiphragma then gently and put down, leave standstill 5 minutes with the sterilization suction pipe.
2) push gently in the middle of the grid zone with finger, moisture is evenly distributed in scraps of paper grid zone, and bubble is driven away.
3) the check scraps of paper that will add sample lie in the 28-35 ℃ of incubator and cultivated 48-72 hour.
4) amplification by the enzyme chromogenic reaction, mould and saccharomycete will show blue spot in growth on the scraps of paper after 24 hours, bigger or a bit diffusion of the spot that mold colony shows, yeast colony is then less and slick and sly, and many moulds can present itself distinctive color in late stage of culture.Select the scraps of paper of clump count moderate (10-100) to count, be mould and saccharomycetic number in every gram (or milliliter) sample after multiply by extension rate.
3, beneficial effect of the present invention:
A large amount of auxiliary works such as cleaning treatment of preparation nutrient culture media, sterilization and cultivation vessel have been saved, at any time can begin to carry out sampling Detection, and easy and simple to handle, by the amplification of enzyme developer, mould and saccharomycetic bacterium colony are clearly displayed in advance.Also saved mould and saccharomycetic incubation time, traditional multistep culture identification process be simplified to one the step finish, incubation time shortens to 48-72 hour by a week.Be not subjected to the restriction of many-sided conditions such as environment, experimental facilities and technician.
(4) accompanying drawing and embodiment
Fig. 1 is the synoptic diagram directly perceived according to a kind of pattern (when raising epiphragma) that the utility model proposes.
Fig. 2 is a cut-open view of the present utility model.
With reference to accompanying drawing, present embodiment is made up of following each several part:
1---epiphragma in the representative,
2---sheet is cultivated in representative,
3---represent egative film.
In the present embodiment: going up epiphragma 1 is that the bottom surface has been coated with the water absorbent gel powder that contains the enzyme developer, the waterproof and breathable, the transparent membrane that have graticule line, its length * wide size is 75 millimeters * 65 millimeters, and the surface is printed on 16 grids, and the size of each grid is 11 millimeters * 11 millimeters; Cultivating sheet 2 is the scraps of paper that adsorbed liquid medium, and its length * wide size is 46 millimeters * 46 millimeters; Egative film 3 is made waterproof and breathable by synthetic material.Last epiphragma 1, cultivation sheet 2 and egative film 3 usefulness viscose glues stick together.
Claims (3)
1, a kind of mould and the saccharomycetic quick test scraps of paper is characterized in that it is coated with last epiphragma that contains enzyme developer water absorbent gel powder and the cultivation sheet that has adsorbed liquid medium and forms by the bottom surface.
2, the mould according to claim 1 and the saccharomycetic quick test scraps of paper is characterized in that epiphragma is waterproof and breathable, the transparent membrane that has graticule line.
3,, under it is characterized in that epiphragma and cultivating sheet egative film is arranged according to claim 1,2 described moulds and the saccharomycetic quick test scraps of paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03274715 CN2646712Y (en) | 2003-09-26 | 2003-09-26 | Quick test sheet paper for fungus and microzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03274715 CN2646712Y (en) | 2003-09-26 | 2003-09-26 | Quick test sheet paper for fungus and microzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2646712Y true CN2646712Y (en) | 2004-10-06 |
Family
ID=34302041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03274715 Expired - Fee Related CN2646712Y (en) | 2003-09-26 | 2003-09-26 | Quick test sheet paper for fungus and microzyme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2646712Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914608A (en) * | 2010-08-09 | 2010-12-15 | 曹莹莹 | Letter pad-type culture medium for rapidly and quantitatively testing flora amount |
| CN101570733B (en) * | 2009-05-12 | 2011-05-18 | 山西省食品工业研究所 | Method for culturing and separating acid-resistant mould fungi |
| CN102471747A (en) * | 2009-07-14 | 2012-05-23 | 大日本印刷株式会社 | Microorganism culture sheet and manufacturing method therefor |
| CN103343158A (en) * | 2013-07-20 | 2013-10-09 | 吉林农业大学 | Detection paper piece for clostridium perfringens, preparation method and application for same |
| CN105483204A (en) * | 2015-12-25 | 2016-04-13 | 贵州勤邦食品安全科学技术有限公司 | Detection vessel for rapidly detecting moulds and yeasts and preparation method |
| CN106811403A (en) * | 2017-01-22 | 2017-06-09 | 贵州勤邦食品安全科学技术有限公司 | A kind of test piece of quick detection bacillus cereus and preparation method thereof, detection method |
-
2003
- 2003-09-26 CN CN 03274715 patent/CN2646712Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101570733B (en) * | 2009-05-12 | 2011-05-18 | 山西省食品工业研究所 | Method for culturing and separating acid-resistant mould fungi |
| CN102471747A (en) * | 2009-07-14 | 2012-05-23 | 大日本印刷株式会社 | Microorganism culture sheet and manufacturing method therefor |
| CN102471747B (en) * | 2009-07-14 | 2014-04-02 | 大日本印刷株式会社 | Microorganism culture sheet and manufacturing method therefor |
| CN101914608A (en) * | 2010-08-09 | 2010-12-15 | 曹莹莹 | Letter pad-type culture medium for rapidly and quantitatively testing flora amount |
| CN103343158A (en) * | 2013-07-20 | 2013-10-09 | 吉林农业大学 | Detection paper piece for clostridium perfringens, preparation method and application for same |
| CN103343158B (en) * | 2013-07-20 | 2014-05-14 | 吉林农业大学 | Detection paper piece for clostridium perfringens, preparation method and application for same |
| CN105483204A (en) * | 2015-12-25 | 2016-04-13 | 贵州勤邦食品安全科学技术有限公司 | Detection vessel for rapidly detecting moulds and yeasts and preparation method |
| CN106811403A (en) * | 2017-01-22 | 2017-06-09 | 贵州勤邦食品安全科学技术有限公司 | A kind of test piece of quick detection bacillus cereus and preparation method thereof, detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Guangzhou Oasis Biochemistry Technology Co., Ltd. Assignor: Oasis Biochemistry Research Center Contract fulfillment period: 2007.6.13 to 2013.9.25 Contract record no.: 2009440001013 Denomination of utility model: Quick test sheet paper for fungus and microzyme Granted publication date: 20041006 License type: Exclusive license Record date: 20090806 |
|
| LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2007.6.13 TO 2013.9.25; CHANGE OF CONTRACT Name of requester: GUANGZHOU OASIS BIOCHEMISTRY TECHNOLOGY CO., LTD. Effective date: 20090806 |
|
| DD01 | Delivery of document by public notice |
Addressee: Lu Xin Document name: Notification to Pay the Fees |
|
| DD01 | Delivery of document by public notice |
Addressee: Oasis Biochemistry Research Center Lu Xin Document name: Notification of Termination of Patent Right |
|
| ASS | Succession or assignment of patent right |
Owner name: GUANGZHOU OASIS BIOCHEMISTRY TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: OASIS BIOCHEMISTRY RESEARCH CENTER Effective date: 20120104 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 510665 GUANGZHOU, GUANGDONG PROVINCE TO: 510663 GUANGZHOU, GUANGDONG PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20120104 Address after: 902 room 7, No. 510663, 1st floor, science Avenue, Guangzhou hi tech Industrial Development Zone, Guangdong, China Patentee after: Guangzhou Oasis Biochemistry Technology Co.,Ltd. Address before: 308, room 510665, west seat, Tong Li building, 491 West Zhongshan Road, Guangzhou, Guangdong Patentee before: Oasis Biochemistry Research Center |
|
| DD01 | Delivery of document by public notice |
Addressee: Lu Xin Document name: Notification of Passing Examination on Formalities |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20041006 Termination date: 20120926 |