CN101693870A - Culture method and device of plant rhizosphere soil microorganism - Google Patents

Abstract

The invention relates to culture method and device of plant rhizosphere soil microorganism. The culture device is a cuboid-shaped container, and the cuboid-shaped container is divided into three parts by two pieces of nylon net, wherein two sides are plant growing chambers, and the middle part is a soil chamber, and the aperture of each piece of nylon net is 30 micrometers; and the culture device is made of non-transparent organic glass plates, or hard plastic plats or PVC plates. The culture method of plant rhizosphere soil microorganism comprises two steps of culturing and sampling. The soil chamber is separated from the plant growing chambers by adopting the two pieces of nylon net to prevent root systems from passing through the plant growing chambers to reach the soil chamber, thereby conveniently separating the plant root systems from the rhizosphere soil; the method has the advantages of convenient sampling, accurate detection and reasonable design.

Description

A kind of plant rhizosphere soil microbial cultivation method and cultivation device

technical field

The invention relates to a soil microorganism cultivation method and a cultivation device, in particular to a plant rhizosphere soil microorganism cultivation method and a cultivation device.

Background technique

The rhizosphere (Rhizosphere) was first proposed by German microbiologist Hiltner in 1904 and defined as the part of the soil micro-area where the microbial population is affected by the root system, generally ranging from 1 to 5 mm from the root surface. Rhizosphere microorganisms are bacteria, actinomycetes, fungi, algae, protozoa and viruses that exist on the root surface and in the surrounding soil. The root exudates of different plants or even different growth stages of the same plant will affect the growth and development of rhizosphere microorganisms. The rhizosphere microorganisms are also involved in various soil physiological and biochemical processes such as the decomposition of soil organic matter, the formation of humus, and the transformation and circulation of nutrients. Therefore, analyzing and comparing the influence of plants on rhizosphere microorganisms can judge and evaluate the influence of plants on soil microenvironment.

A large number of microorganisms in the soil usually grow in an unstable dynamic balance, and plant roots are an important factor that leads to drastic changes in soil microorganisms. Therefore, research on rhizosphere microbes has also attracted much attention. Since the 1970s, international research on rhizosphere microbiology has been quite active, and the chemical process at the root-soil interface has become a research hotspot. However, for the sampling method of rhizosphere soil, traditional research mostly adopts artificial shaking separation method. This method has defects such as uneven sampling volume under different plant species, soil types, and field humidity, etc., and it is easy to cause a small amount of root tissue, especially root hairs, to affect the analysis and determination of rhizosphere soil microbial biomass. accuracy of the test.

Contents of the invention

The invention overcomes the deficiencies of the prior art and provides a scientific, reasonable and accurate method for cultivating plant rhizosphere soil microorganisms and a corresponding cultivating device.

In order to solve the above-mentioned technical problems, the present invention adopts the following technical solutions:

A culture device for soil microorganisms in the rhizosphere of plants, the culture device is a cuboid container, the cuboid container is divided into three parts by two nylon nets, wherein the two sides are plant growth chambers, and the middle is a soil chamber. The pore size of the nylon net is 30 μm, and the culture device adopts a non-transparent plexiglass plate or a hard plastic plate or a PVC plate.

A method for cultivating plant rhizosphere soil microorganisms, comprising the steps of:

1. Cultivation: mix the soil and fertilizer through a 1mm sieve, and pack them into the plant growth chamber and the soil chamber in the cultivation device according to claim 1 respectively. The soil is 1.5cm away from the edge of the cultivation device, and then watered to make the weight of the whole container The water content is 16% to 20%. The surface of the plant seeds is sterilized with 10% sodium hypochlorite, and then rinsed with deionized water for 3 times. The seeds are put into a petri dish at 25°C in the dark for 1 to 2 days, and finally sowed on the plants. In the growth chamber, the water in the plant growth chamber and the soil chamber is supplemented by weighing method during the plant growth process, so that the water content by weight in the whole container is maintained at 16% to 20%.

2. Sampling: When cultivating for 6-8 weeks, separate the soil on the nylon net, keep the soil in the soil room as it is, and take soil samples at a distance of 0-5mm from the plant growth room. This part of the soil is the rhizosphere soil. Follow this method Soil samples can be taken for index determination of soil microorganisms.

In the present invention, the use of the nylon net can prevent the root system from passing through the plant growth chamber to reach the soil chamber, can conveniently separate the plant root system from the root soil, has convenient sampling, accurate measurement and reasonable design.

Description of drawings

Fig. 1 is a schematic structural view of the culture device of the present invention.

Detailed ways

The present invention will be further elaborated below in conjunction with the accompanying drawings.

As shown in accompanying drawing 1, a kind of cultivation device of plant rhizosphere soil microorganism, described cultivation device is a cuboid container, and described cuboid container is divided into three parts by two pieces of nylon mesh 3, wherein both sides are plant growth Chambers 1 and 1' have a soil chamber 2 in the middle, the nylon mesh has a pore size of 30 μm, and the cultivation device uses a non-transparent plexiglass plate or a hard plastic plate or a PVC plate.

A method for cultivating plant rhizosphere soil microorganisms, comprising the steps of:

1. Cultivation: mix the soil and fertilizer through a 1mm sieve, and put them into the plant growth chamber 1, 1' and soil chamber 2 in the cultivation device according to claim 1 respectively, the soil is 1.5cm away from the edge of the cultivation device, and then water Make the weight water content of the whole container 16%-20%, sterilize the surface of the plant seeds with 10% sodium hypochlorite, then rinse with deionized water 3 times, put the seeds into a petri dish at 25°C in dark conditions for 1-2 days to accelerate germination , and finally sow the seeds in the plant growth chamber, and use the weighing method to supplement the water in the plant growth chamber and the soil chamber during the plant growth process, so that the weight water content in the entire container is maintained at 16% to 20%.

2. Sampling: When cultivating for 6-8 weeks, separate the soil at 3 places of the nylon mesh, keep the soil in the soil room as it is, and take soil samples at a distance of 0-5 mm from the plant growth room. This part of the soil is the rhizosphere soil. According to this Methods Soil samples were taken to measure the indicators of soil microorganisms.

Claims (2)

1. a culture device of plant rhizosphere soil microorganism, described culture device is a cuboid container, it is characterized in that: described cuboid container is separated into three parts by two nylon nets, wherein both sides are plant growth chambers, The soil chamber is in the middle, and the pore size of the nylon mesh is 30 μm, and the cultivation device adopts a non-transparent plexiglass plate or a rigid plastic plate or a PVC plate. 2. a cultivation method of plant rhizosphere soil microorganism, it is characterized in that comprising the steps: 1. Cultivation: mix the soil and fertilizer through a 1mm sieve, and pack them into the plant growth chamber and the soil chamber in the cultivation device according to claim 1 respectively. The soil is 1.5cm away from the edge of the cultivation device, and then watered to make the weight of the whole container The water content is 16% to 20%. The surface of the plant seeds is sterilized with 10% sodium hypochlorite, and then rinsed with deionized water for 3 times. The seeds are put into a petri dish at 25°C in the dark for 1 to 2 days, and finally sowed on the plants. In the growth chamber, the water in the plant growth chamber and the soil chamber is supplemented by weighing method during the plant growth process, so that the weight water content in the whole container is kept at 16% to 20%; 2. Sampling: When cultivating for 6-8 weeks, separate the soil on the nylon net, keep the soil in the soil room as it is, and take soil samples at a distance of 0-5mm from the plant growth room. This part of the soil is the rhizosphere soil. Follow this method Soil samples can be taken for index determination of soil microorganisms.

Images