CN203117162U - Integrated membrane biosensor for quickly detecting escherichia coli - Google Patents
Integrated membrane biosensor for quickly detecting escherichia coli Download PDFInfo
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- CN203117162U CN203117162U CN201320161422.8U CN201320161422U CN203117162U CN 203117162 U CN203117162 U CN 203117162U CN 201320161422 U CN201320161422 U CN 201320161422U CN 203117162 U CN203117162 U CN 203117162U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Abstract
本实用新型涉及一种微生物检测技术领域，具体的说是一种检测大肠杆菌的生物传感器。一种快速检测大肠杆菌的一体化薄膜生物传感器，所述的树脂片镀制有一层合金膜，支撑结构检测层上沉积有一层培养基层，玻璃试管内可灌入碳糊，其玻璃试管一口处开有注浆孔，玻璃试管两端口各设有铜探针，铜探针通过连接导线与智能循环伏安扫描一体机相连通，智能循环伏安扫描一体机输入端口还与合金膜相连通。本实用新型解决了对细菌检测操作复杂，且需要花费时间长的问题，提供了一种容易制备且测试操作简单方便、灵敏度高、特异性强、响应时间短、可定量检测细菌浓度的生物传感器，实现了对细菌的直接检测。
The utility model relates to the technical field of microorganism detection, in particular to a biosensor for detecting Escherichia coli. An integrated thin-film biosensor for rapid detection of Escherichia coli, the resin sheet is coated with a layer of alloy film, a layer of culture medium is deposited on the detection layer of the support structure, carbon paste can be poured into the glass test tube, and the glass test tube has a There are grouting holes, and copper probes are provided at the two ports of the glass test tube. The copper probes are connected to the intelligent cyclic voltammetry scanning integrated machine through connecting wires, and the input port of the intelligent cyclic voltammetric scanning integrated machine is also connected to the alloy film. The utility model solves the problem that the bacteria detection operation is complicated and takes a long time, and provides a biosensor that is easy to prepare, has simple and convenient test operation, high sensitivity, strong specificity, short response time, and can quantitatively detect the concentration of bacteria , to achieve direct detection of bacteria.
Description
Technical field
The utility model relates to a kind of microorganism detection technical field, specifically the colibacillary integrated thin film bio sensor of a kind of fast detecting.
Background technology
Biology sensor is a kind of bio signal to be transformed into the instrument of electric signal, is made of bio-identification system and signal transmission system usually.Clark is taught in 1956 and has delivered one piece of article about oxygen electrode, for important basis has been established in the development of biology sensor.Biology sensor has the specific biological molecule distinguishability, with a kind of responsive converter incorporates, detects analyte with high selectivity and sensitivity, becomes fast, the important method of material in the environment such as Sensitive Detection water body, atmosphere.According to the sensitive materials difference on the used molecular recognition elements in the biology sensor, biology sensor can be divided into enzyme sensor, microbiological sensor, tissus sensor, cell organelle sensor, immunosensor and DNA biology sensor etc.Oxygen content is closely bound up in the aerobic respiration process of bacterium and sweat and the environment, growth and breeding that can be by the monitoring bacterium and then carry out living things system redox monitoring, environmental pollution monitoring.
And well-known microorganism is closely related with human life, so they have very significant effects for the mankind's life.For example, many bacteriums can discharge toxin causes a disease people and animals, so but also there are many bacteriums to be utilized by we mankind simultaneously, this is commonly referred to probio.At present, the normally physiology by measuring bacterium, morphological feature or identify bacterium by the genomic constitution of measuring bacterium of the method for general bacterial detection.These methods complexity that operates sees, and needs the cost long time, and they can not satisfy current requirement to microorganism detection far away.In this case, many researchists explore new method hardy, in order to bacterium is detected rapidly.People such as Charach in 1993 reported first on the science utilize have cell surface be subjected to poly-two alkyne films of stopping with corresponding ligand molecular in conjunction with the time, the color of film can have been carried out direct colorimetric detection to influenza virus by the red this phenomenon of blue stain.This affinity metachromatism has caused people's extensive interest.Domestic have many units that this is studied, but these researchs all concentrate on and utilize the optical property of poly-two alkynes that bacterium is detected, and the report that utilizes electrochemical method bacterial detection also seldom.
Escherichia coli are foremost a kind of bacteriums in the humans and animals enteron aisle, and main parasitic accounts for 1% in the enteron aisle bacterium in large intestine.Be the blunt circle in a kind of two ends, can move, nonspore-bearing Gram-negative brevibacterium.Escherichia coli energy synthetic vitamin B and K, not pathogenic under the normal perch condition; Locate and to cause inflammation if enter gall-bladder, bladder etc.In water and food, detect, can think by the index of fecal pollution.The hygiene standard of coliform group count Chang Zuowei drinking-water, food or medicine.
The utility model adopts electrochemical detection method to set out at Escherichia coli, developed a kind ofly have that checkout equipment is simple, volume is little, be quick on the draw, biology sensor that detection speed is fast.
Summary of the invention
The purpose of this utility model provides the colibacillary integrated thin film bio sensor of a kind of fast detecting, thereby fills up in the existing market blank for Electrochemical Detection mushroom instrument and equipment.
The utility model solves its above-mentioned technical matters and adopts following technical scheme: the colibacillary integrated thin film bio sensor of a kind of fast detecting, it mainly is configured with: resin sheet, alloy film, the supporting construction detection layers, culture medium layer, carbon paste, grouting hole, the copper probe, connect lead, intelligent circulation volt-ampere scanning integrated machine, glass test tube, described resin sheet has been coated with one deck alloy film, deposit one deck culture medium layer on the supporting construction detection layers, can pour into carbon paste in the glass test tube, its glass test tube locates to have grouting hole flatly, the glass test tube two-port respectively is provided with the copper probe, the copper probe is connected with intelligent circulation volt-ampere scanning integrated machine by connecting lead, and intelligent circulation volt-ampere scanning integrated machine input port also is connected with alloy film.
Its thickness of above-mentioned alloy film is at 2 ~ 4mm.
Its thickness of above-mentioned culture medium layer is at 8 ~ 15mm.
Above-mentioned glass test tube and culture medium layer surface of contact place have pore.
The optical property of the poly-two alkynes of the utility model microbiological sensor and common utilization is carried out detection method to bacterium and is compared and have the following advantages: solved the Bacteria Detection complicated operation, and need the long problem of spended time, provide that a kind of easy preparation and test operation are simple and convenient, highly sensitive, high specificity, response time are short, the quantitative biology sensor of bacterial detection concentration, have realized the direct detection to bacterium.The utility model can detect bacterium rapidly, all has very important meaning at medical diagnosis, food industry and field of environment protection.
Description of drawings
Fig. 1 is the colibacillary integrated thin film bio sensor one-piece construction synoptic diagram of a kind of fast detecting of the utility model.
1-resin sheet among the figure, the 2-alloy film, 3-supporting construction detection layers, the 4-culture medium layer, the 5-carbon paste, the 6-grouting hole, 7-copper probe, 8-connects lead, 9-intelligent circulation volt-ampere scanning integrated machine, 10-glass test tube.
Embodiment
Make a detailed explanation below in conjunction with 1 pair of embodiment of the present utility model of accompanying drawing.
Embodiment: the colibacillary integrated thin film bio sensor of a kind of fast detecting, it mainly is configured with: resin sheet 1, alloy film 2, supporting construction detection layers 3, culture medium layer 4, carbon paste 5, grouting hole 6, copper probe 7, connect lead 8, intelligent circulation volt-ampere scanning integrated machine 9, glass test tube 10, described resin sheet 1 has been coated with one deck alloy film 2, deposit one deck culture medium layer 4 on the supporting construction detection layers 3, can pour into carbon paste 5 in the glass test tube 10, its glass test tube 10 has grouting hole 6 in the place flatly, glass test tube 10 two-port respectively are provided with copper probe 7, copper probe 7 is connected with intelligent circulation volt-ampere scanning integrated machine 9 by connecting lead 8, and intelligent circulation volt-ampere scanning integrated machine 9 input ports also are connected with alloy film 2.
Described alloy film 2 its thickness are at 2 ~ 4mm.The platinum of its alloy film 2 employings 35% and 65% silver are formed.
Described culture medium layer 4 its thickness are at 8 ~ 15mm.Its culture medium layer 4 is mannose or sialic acid.
Described glass test tube 10 has pore with culture medium layer 4 surface of contact places.
Do the explanation of an example below at whole coli test cultivation, testing process:
One, colibacillary cultivation:
After beef-protein medium, Yihong, Mei Lan and the used water of configuration nutrient culture media sterilized about 5 hours under 121 ℃ condition, take out successively and be cooled to room temperature, Escherichia coli (E. coli) are inoculated into fill the beef peptone nutrient culture media in vitro then.Postvaccinal test tube is positioned in the shaking table, and regulates incubator temperature to 37.5 ℃, cultivate 24 h.The peptone, water, Yihong, methylene blue, the nutrient culture media that take out after sterilizing are cooled to room temperature, on the sterile working platform they are respectively charged in the flat board, and the flat board that will install is placed on the place of lucifuge with stand-by at last.
Two, will sneak in the carbon paste after the cultured Escherichia coli dilution:
The Escherichia coli original solution is diluted to 10
-5, 10
-6, 10
-7, trapped fluid is diluted to 10
-5, 10
-6, 10
-7, 10
-8, then the solution that dilutes above and reserve liquid and keep stand-by.Solution after the dilution under the variable concentrations and trapped fluid are respectively got 0.1mL, put into respectively on the cultured different nutrient culture media and shakeout.It should be noted that in whole experiment all operations all need be in desinfection chamber and the operation of spirit lamp flame next door.The nutrient culture media that has coated together is placed in the incubator, adjusts the temperature of incubator it is cultivated.After incubation time reaches 24h, can count the clump count on the nutrient culture media.
Dag and epoxy resin are mixed the back in proportion inject some in glass test tube 10 by grouting hole 6, cultured Escherichia coli and a small amount of nutrient culture media, carbon dust and methyl-silicone oil are mixed into carbon paste 5, the carbon paste 5 that obtains is filled in the loculus of full glass test tube 10, make the carbon paste working electrode that Escherichia coli are modified.
Three, equipment experiment beginning and data conclusion thereof:
Escherichia coli belong to amphimicrobian type bacterium, carry out aerobic respiration under (PBS, pH 7. 0) aerobic conditions in suitable phosphate buffered solution, can carry out anaerobic respiration or fermentation under the oxygen free condition.The carbon paste that comprises bacterium 5 of preparation is started working by copper probe 7 electrodes, because intelligent circulation volt-ampere scanning integrated machine 9 is connected with alloy film 2, copper probe 7 electrodes, therefore when started by working electrodes, its intelligent circulation volt-ampere scanning integrated machine 9 can record colibacillary existence by cyclic voltammetry, and relevant quantity or concentration.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320161422.8U CN203117162U (en) | 2013-04-02 | 2013-04-02 | Integrated membrane biosensor for quickly detecting escherichia coli |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320161422.8U CN203117162U (en) | 2013-04-02 | 2013-04-02 | Integrated membrane biosensor for quickly detecting escherichia coli |
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| Publication Number | Publication Date |
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| CN203117162U true CN203117162U (en) | 2013-08-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201320161422.8U Expired - Fee Related CN203117162U (en) | 2013-04-02 | 2013-04-02 | Integrated membrane biosensor for quickly detecting escherichia coli |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103822949A (en) * | 2014-01-14 | 2014-05-28 | 济南大学 | Preparation method and application of electrochemical immunosensor for detecting escherichia coli |
| CN108562631A (en) * | 2018-03-19 | 2018-09-21 | 青岛科技大学 | A kind of test method detecting anaerobic digestion based on electrochemical techniques |
-
2013
- 2013-04-02 CN CN201320161422.8U patent/CN203117162U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103822949A (en) * | 2014-01-14 | 2014-05-28 | 济南大学 | Preparation method and application of electrochemical immunosensor for detecting escherichia coli |
| CN108562631A (en) * | 2018-03-19 | 2018-09-21 | 青岛科技大学 | A kind of test method detecting anaerobic digestion based on electrochemical techniques |
| CN108562631B (en) * | 2018-03-19 | 2020-09-01 | 青岛科技大学 | Test method for detecting anaerobic digestion based on electrochemical technology |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130807 Termination date: 20140402 |