CN209751293U - A tool for embryo transfer - Google Patents

Abstract

The utility model discloses an embryo transplantation tool, which comprises an oviposit tube and a casing. The oviposit tube includes a catheter needle and a joint. The two ends of the casing are respectively set as a thick end and a thin end. It gradually becomes smaller to the thin end; the inner and outer walls of the casing are smooth, and the openings at the upper and lower ends are round. The embryo transfer tool disclosed by the utility model is helpful to quickly and easily find the opening of the fallopian tube umbrella, and has low requirements for operating skills, even those who have no experience in embryo transfer can operate skillfully; The small size reduces the infection risk of the recipient due to pelvic bleeding and adhesion, not only improves the success rate of transplantation and the utilization efficiency of the recipient, but also reduces the production cost.

Description

A tool for embryo transfer

technical field

The utility model relates to the technical field of embryo transfer, in particular to an embryo transfer tool.

Background technique

Embryo transfer in mammals began in the 1930s, and the success of pig embryo transfer experiments was first reported by Kvashickii in 1951. By the 1960s, the technology of surgical method to collect and transfer embryos had been basically established. Many pig cloning experiments have been carried out at home and abroad. According to statistics, the cloning efficiency of pigs is basically maintained at 0.5%-3%, and the success rate of embryo transfer is an important factor affecting the efficiency of cloning pigs.

When carrying out pig embryo transfer, whether to find and open the opening of the fallopian tube umbrella quickly is a key link in embryo transfer. In practice, in order to minimize unnecessary damage to the fallopian tubes, it often takes a long time to find the opening of the umbrella of the fallopian tube. After finding the opening, the embryo transfer tube needs to be inserted into the fallopian tube very carefully. This process is likely to cause secondary damage to the fallopian tubes or uterus, leading to problems such as recipient pelvic bleeding and uterine adhesions, which will not only affect the success rate of transplantation, reduce the utilization efficiency of recipients, but also increase production costs.

Utility model content

The purpose of the utility model is to provide an embryo transfer tool with simple and fast operation and little trauma to the oviduct of the sow, so as to solve the problems of complicated operation of the embryo transfer tool and great trauma to the sow.

The embryo transfer tool provided by the utility model includes a transfer tube, a sleeve and a syringe. The transfer tube includes a catheter needle and a joint, and the joint is fixedly connected with the end of the catheter needle. The outer diameter of the catheter needle is Less than 2.00mm, the outer diameter of the catheter needle is 0.30 to 2.00mm, and the length of the catheter needle is greater than 12.00cm; the two ends of the sleeve are respectively set as thick ends and thin ends; the inner diameter of the sleeve is greater than the outer diameter of the catheter needle and less than or equal to the outer diameter of the joint; the inner and outer walls of the sleeve are smooth, and the openings at the upper and lower ends are round; the catheter needle of the oviduct The thick end of the tube is passed through and passed out from the thin end to expose the needle of the catheter needle, and the joint of the oviduct is blocked by the thin end of the cannula.

Preferably, the outer diameter of the sleeve gradually decreases from the thick end to the thin end.

Preferably, the embryo transfer tool includes a syringe, and the syringe is arranged on the joint.

Preferably, the syringe is a 1 mL syringe.

Preferably, the oviduct is a plastic tube.

Preferably, the inner and outer walls of the oviduct are smooth, and the openings at the upper and lower ends are round.

Preferably, the total length of the oviduct tube is 14.00cm, the outer diameter of the joint of the oviduct tube is 6.00mm, and the inner diameter is 4.00mm, and the catheter needle of the oviduct tube has an outer diameter of 1.50mm and an inner diameter of 0.50mm.

Preferably, the sleeve is a glass sleeve.

Preferably, the lower end of the casing is sharp, and the upper end is round and thick in a tapered shape.

The inner diameter of the thick end of the sleeve is sleeved on the outer diameter of the joint, preferably, the length of the sleeve is 3.00cm, the inner diameter of the thick end is 8.00mm, and the outer diameter is 9.00mm; the thin end The inner diameter is 4.00mm and the outer diameter is 5.00mm.

Compared with the prior art, the utility model has the following advantages: the utility model is provided with an oviduct and a cannula with a smooth tube wall and a round port. The opening of the oviduct is established to establish the entry channel of the oviduct, and then the embryo is sucked through the syringe set at the upper end of the oviduct, and finally the oviduct is inserted into the oviduct along the cannula until it enters the depth of the oviduct and the embryo is injected into the oviduct. The embryo transfer tool disclosed by the utility model is helpful to quickly and easily find the opening of the fallopian tube fimbria, has low requirements for operating skills, and can be skillfully operated even by those who have no experience in embryo transfer; The small size reduces the infection risk of the recipient due to pelvic bleeding and adhesion, not only improves the success rate of transplantation and the utilization efficiency of the recipient, but also reduces the production cost.

Description of drawings

FIG. 1 is a schematic diagram of an embodiment of the embryo transfer tool provided in Example 1.

FIG. 2 is a schematic structural view of the oviduct tube provided in Example 1. FIG.

FIG. 3 is a schematic structural view of the casing provided in Example 1. FIG.

FIG. 4 is a schematic diagram of another embodiment of the embryo transfer tool provided in Example 2.

Detailed ways

Example 1

Embodiment 1 provides an embryo transfer tool, and the structure provided in Embodiment 1 will be described in detail below.

Referring to FIG. 1 , the embryo transfer tool includes an oviduct 1 and a sleeve 2 , and a connector 11 is fixedly connected to an end of a catheter needle 12 .

Referring to Fig. 2, the total length of the oviduct 1 is 14.00cm, and the length of the catheter needle 12 is greater than 12.00cm; The needle 12 has an outer diameter of 1.50 mm and an inner diameter of 0.50 mm.

Specifically, the oviduct tube 1 is a plastic tube, and preferably, its material is PVC plastic resistant to a high temperature of 190°C. The inner and outer tube walls of the oviduct 1 are smooth, and the openings at the upper and lower ends are round.

Referring to FIG. 3 , the two ends of the sleeve 2 are respectively configured as a thick end 21 and a thin end 22 , and the outer diameter of the sleeve 2 gradually decreases from the thick end 21 to the thin end 22 .

In order to realize that the inner diameter of the thick end 21 of the cannula 2 is sleeved on the outer diameter of the joint 11, the inner diameter of the cannula 2 is larger than the outer diameter of the catheter needle 12 and less than or equal to the outer diameter of the joint 11; the inner and outer walls of the cannula 2 Smooth, with rounded upper and lower openings.

Specifically, the sleeve 2 is a glass sleeve, preferably made of high temperature resistant quartz glass. The casing 2 is tapered, with round openings at the upper and lower ends. The length of the casing 2 is 3.00 cm, the inner diameter of the butt end 21 is 8.00 mm, and the outer diameter is 9.00 mm.

Referring to Fig. 1, the catheter needle 12 of the oviduct tube 1 passes through the thick end 21 of the sleeve tube 2 and passes out from the thin end 22, exposing all the needles of the catheter needle 12, and the joint 11 of the oviduct tube 1 is covered by the thin end of the sleeve tube 2. End 22 blocks.

Example 2

On the basis of Example 1, Example 2 improves the connector 11 of the oviduct tube 1 to provide another embryo transfer tool, the improvement is:

Referring to FIG. 4 , the embryo transfer tool includes a syringe 3 , and the syringe 3 is arranged on the joint 11 of the oviduct tube 1 . The setting method includes various methods such as plugging, threaded connection, clamping, socketing, etc., and even includes the integral formation of the syringe 3, the joint 11 and the catheter needle 12. In the socketing method, flexible rubber tubes can be used to connect the syringes respectively. 3 output and connector 11.

Preferably, the syringe is a commercially available 1mL syringe.

Example 3

Embodiment 3 provides a kind of embryo transfer method, adopts the embryo transfer tool that embodiment 2 provides, and this embryo transfer method comprises the following steps:

Step S1: provide recipient sows, and general anesthesia;

Step S2: setting a surgical incision 100 on the abdomen of the recipient sow, and leading the oviduct, ovary and part of the uterus out of the incision 100;

Step S3: Insert the thin end 22 of the cannula 2 into the opening 100 of the fimbria of the oviduct of the sow;

Step S4: setting a 1 mL syringe 3 on the joint 11 of the oviduct tube 1;

Step S5: using the syringe 3 to suck the embryo to be transplanted into the catheter needle 12 in the oviduct;

Step S6: Insert the catheter needle 12 of the oviduct 1 through the thick end 21 of the cannula 2 and out from the thin end 22, and continuously extend the needle of the catheter needle 12 until it cannot go further;

Step S7: Using a syringe, push the embryo from the oviduct 1 into the oviduct of the recipient.

Step S8: After the injection is completed, the oviduct 1 and the cannula 2 are sequentially drawn out, the oviduct and ovary of the sow are reset, and the wounded incision 100 is sutured.

Although the utility model has been described in detail with general description and specific examples above, some modifications or improvements can be made on the basis of the utility model, which will be obvious to those skilled in the art. Therefore, the modifications or improvements made on the basis of not departing from the spirit of the utility model all belong to the protection scope of the utility model.

Claims (9)

1. an embryo transfer tool, is characterized in that, described embryo transfer tool comprises oviduct (1) and sleeve pipe (2), The oviduct (1) includes a connector (11) and a catheter needle (12), the connector (11) is fixedly connected to the end of the catheter needle (12), and the outer diameter of the catheter needle (12) is is 0.30 to 2.00mm, and the length of the catheter needle (12) is greater than 12.00cm; The two ends of the sleeve (2) are respectively set as a thick end (21) and a thin end (22); The inner diameter of the sleeve (2) is greater than the outer diameter of the catheter needle (12) and less than or equal to the outer diameter of the joint (11); the inner and outer walls of the sleeve (2) are smooth, the upper end and The opening at the lower end is round; The catheter needle (12) of the oviduct (1) penetrates through the thick end (21) of the cannula (2) and passes out from the thin end (22), exposing the needle of the catheter needle (12), so The joint (11) of the oviduct (1) is blocked by the thin end (22) of the sleeve (2). 2. The embryo transfer tool according to claim 1, characterized in that, The outer diameter of the sleeve (2) gradually decreases from the thick end (21) to the thin end (22). 3. The embryo transfer tool according to claim 1, characterized in that the embryo transfer tool comprises a syringe (3), and the syringe (3) is arranged on the joint (11). 4. The embryo transfer tool according to claim 3, characterized in that, the syringe (3) is a 1 mL syringe. 5. The embryo transfer tool according to claim 1, characterized in that, the oviposit tube (1) is a plastic tube. 6. The embryo transfer tool according to claim 1, characterized in that, the inner and outer walls of the oviduct (1) are smooth, and the openings at the upper and lower ends are round. 7. The embryo transfer tool as claimed in claim 1, characterized in that, the total length of the oviposit tube (1) is 14.00cm, and the outer diameter of the joint (11) of the oviposit tube (1) is 6.00 cm. mm and an inner diameter of 4.00mm, the catheter needle (12) of the oviduct (1) has an outer diameter of 1.50mm and an inner diameter of 0.50mm. 8. The embryo transfer tool according to claim 1, characterized in that, the sleeve (2) is a glass sleeve. 9. The embryo transfer tool as claimed in claim 1, characterized in that, the length of the sleeve pipe (2) is 3.00cm, and the inner diameter of the butt end (21) is 8.00mm, and the outer diameter is 9.00mm; The inner diameter of the thin end (22) is 4.00mm, and the outer diameter is 5.00mm.