CN222444432U - Egg detecting and transplanting device - Google Patents

Abstract

The utility model relates to an egg detecting and transplanting device which comprises a rubber tube, a suction nozzle, a filter element gun head, a needle body fixer, a glass needle and a cotton plug, wherein the suction nozzle is connected with one end of the rubber tube, and the needle body fixer is connected with the other end of the rubber tube. The filter core gun head is connected to the suction nozzle, the glass needle is arranged on the needle body fixer, the cotton plug is arranged in the rubber tube, and the cotton plug is contacted with the end part of the glass needle. When the device is used for working, fertilized eggs are sucked or moved out of the glass needle by utilizing oral cavity suction in the process of egg detection and transplantation, so that egg detection and transplantation operation are realized, the device can well solve the problem of cross infection caused by oral cavities and suction nozzles through the filter element gun head, and meanwhile, when the fertilized eggs are sucked, if part of liquid enters the rubber tube from the glass needle due to overhigh suction force, the liquid can be adsorbed by the cotton plug, and the sucked liquid is prevented from directly contacting the rubber tube, so that the sanitary problem can be well solved.

Description

Egg detecting and transplanting device

Technical Field

The utility model relates to the technical field of mouse embryo transplantation, in particular to an egg detecting and transplanting device.

Background

The mouse in vitro fertilization-embryo transfer technology is widely applied to the fields of mouse embryo purification, cryopreservation, resuscitation, rapid propagation, transgenic mouse preparation and the like. The straw is an essential experimental tool for the steps of egg detection (egg washing, egg transferring) and embryo transplantation in the process of in vitro fertilization-embryo transplantation, and is an oral straw or a manual control tube which is currently used.

Wherein, traditional mouth straw comprises hose and glass needle of connection suction nozzle, and oral cavity and suction nozzle direct contact easily cause cross contamination.

The hand control tube is mainly composed of a hose and a glass needle which are sealed on one side in the early stage, and is composed of a hose and a glass needle with a suction head in the later stage, or is composed of a suction head and a glass needle. The early hand control tube is more sanitary than the traditional oral suction tube, but can only be used for other operation processes except embryo transplantation, has limited use occasions, and the improved hand control tube has short pipeline length, fast liquid rising and easy contact with the suction head or the hose.

Disclosure of Invention

In order to solve the problems, the utility model provides an egg detecting and transplanting device which can avoid cross infection between an oral cavity and a suction nozzle, can prevent liquid from contacting a rubber hose by arranging a cotton plug, and has the advantage of higher practicability.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

An egg and graft device, comprising:

a rubber tube;

The suction nozzle is connected with one end of the rubber tube;

the filter element gun head is connected to the suction nozzle;

The needle body fixer is connected to the other end of the rubber tube;

A glass needle provided on the needle body holder, and

And the cotton plug is arranged in the rubber tube and is contacted with the end part of the glass needle.

In one embodiment, a filter is connected to the middle of the rubber tube.

In one embodiment, the glass needle comprises:

a holding section for mounting connection with the needle holder, and

And one end of the capillary section is connected with the holding section, the other end of the capillary section is a needle point, and the needle point is in conical transition.

In one embodiment, the opening of the needle tip is round and smooth.

In one embodiment, the inner diameter of the needle opening is 100-120um, and the inner diameter of the capillary section is 200-300 um.

In one embodiment, the glass needle comprises:

a holding section for mounting connection with the needle holder, and

The capillary section, the one end of capillary section with hold the section and be connected, the other end is the needle point, the needle point with capillary section is the deflection setting, and deflection angle is 15-30 degrees.

In one embodiment, the capillary segment has an inner diameter of 100-120um.

In one embodiment, the tip has a deflection length of 1mm.

In one embodiment, the opening of the needle tip is round and smooth.

Due to the adoption of the technical scheme, the utility model has the following advantages:

1. When the device is used for working, the glass needle is mounted in the needle body fixer, the filter core gun head is clamped and connected with the suction nozzle, then the filter core gun head is held in the mouth, meanwhile, the needle body fixer is held by the right hand, fertilized eggs are sucked or moved out of the glass needle by utilizing the suction force of the mouth in the egg checking and transplanting process, so that the egg checking and transplanting operation is realized, compared with the prior art, the device can well solve the problem of cross infection caused by the mouth and the suction nozzle through the filter core gun head, and meanwhile, when the fertilized eggs are sucked, if the suction force is too high, part of liquid is absorbed by the cotton plug when entering into the rubber tube from the glass needle, the sucked liquid is prevented from being directly contacted with the rubber tube, the sanitary problem can be well solved, and the device has the advantage of higher practicability;

2. The filter is mainly used for filtering air in the rubber hose, and can slow down the flow rate of gas in the filtering process, so that the phenomenon that liquid rises too fast is avoided, and the filter is convenient to fix on the microscope eye tube temporarily.

Drawings

FIG. 1 is a schematic diagram showing a specific structure of an egg testing and transplanting device according to an embodiment of the present utility model;

FIG. 2 is a schematic view of a glass needle for egg detection in accordance with an embodiment of the present utility model;

FIG. 3 is a schematic view of a glass needle according to an embodiment of the present utility model;

FIG. 4 is a schematic view showing the structure of a glass needle for implantation according to an embodiment of the present utility model;

FIG. 5 is a schematic view of a deflection structure of a needle in accordance with one embodiment of the present utility model;

The figures are marked as follows:

1. A cartridge gun head; 2, a suction nozzle, 3, an upper rubber tube, 4, a filter, 5, a lower rubber tube, 6, a cotton plug, 7, a needle body fixer, 8, a glass needle, 9, a holding section, 10, a capillary section, 11 and a needle tip.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions in the present utility model will be clearly and completely described below, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are intended to be within the scope of the present disclosure.

Unless defined otherwise, technical or scientific terms used herein should be given the ordinary meaning as understood by one of ordinary skill in the art to which this utility model belongs. The terms "first," "second," "third," "fourth," and the like as used herein do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that elements or items preceding the word are included in the element or item listed after the word and equivalents thereof, but does not exclude other elements or items. The terms "connected" or "connected," and the like, are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.

The straw is an essential experimental tool for the steps of egg detection (egg washing, egg transferring) and embryo transplantation in the process of in vitro fertilization-embryo transplantation, and is an oral straw or a manual control tube which is currently used. Wherein, traditional mouth straw comprises hose and glass needle of connection suction nozzle, and oral cavity and suction nozzle direct contact easily cause cross contamination. Compared with the traditional oral suction tube, the hand control tube is more sanitary, but can only be used for other operation processes except embryo transplantation, has limited use occasions, and the improved hand control tube has short pipeline length, fast liquid rising and easy contact with the suction head or the hose. In view of the above problems, the utility model provides an egg detecting and transplanting device, which can avoid cross infection between an oral cavity and a suction nozzle, can prevent liquid from contacting a rubber hose by arranging a cotton plug, and has the advantage of higher practicability.

Referring to fig. 1, the egg detecting and transplanting device comprises a rubber tube, a suction nozzle 2, a filter element gun head 1, a needle body fixer 7, a glass needle 8 and a cotton plug 6, wherein the suction nozzle 2 is connected with one end of the rubber tube, and the needle body fixer 7 is connected with the other end of the rubber tube. The filter core gun head 1 is connected to the suction nozzle 2, the glass needle 8 is arranged on the needle body fixer 7, the cotton plug 6 is arranged inside the rubber tube, and the cotton plug 6 is contacted with the end part of the glass needle 8.

In this embodiment, the cartridge gun head 1 is a disposable product, the cartridge gun head 1 is directly clamped into the suction nozzle 2 to be connected, and except for the connection between the cartridge gun head 1 and the suction nozzle 2, the interfaces between other parts are all made of rubber hoses and are connected with plastic or rubber, so that the connection is tight and not easy to fall off.

The device is used for working, the glass needle 8 is arranged in the needle body fixer 7, the filter element gun head 1 is clamped into the suction nozzle 2 to be connected, then the filter element gun head 1 is held in the oral cavity, meanwhile, the needle body fixer 7 is held by the right hand, fertilized eggs are sucked or moved out of the glass needle 8 by utilizing oral suction in the egg detection and transplantation process, so that egg detection and transplantation operation are realized, compared with the prior art, the device can well solve the problem of cross infection caused by the oral cavity and the suction nozzle 2 through the filter element gun head 1, and meanwhile, when the fertilized eggs are sucked, if the suction force is too high, part of liquid is adsorbed by the cotton plug 6 when the liquid enters the rubber tube from the glass needle 8, the sucked liquid is prevented from directly contacting the rubber tube, the sanitary problem can be well solved, and the device has the advantage of higher practicability.

In an embodiment, it is more preferable that a filter 4 is connected to the middle of the rubber tube, and the filter 4 divides the rubber tube into an upper rubber tube 3 and a lower rubber tube 5.

It should be noted that, the filter 4 is mainly used for filtering air in the rubber hose, and can slow down the flow rate of gas in the filtering process, avoid preventing that liquid from rising too fast, be convenient for temporarily place the fixation on the microscope eye tube simultaneously.

Wherein, in the in-process of whole experimental operation, need intermittent other steps of doing, need place whole device temporarily aside this moment, can hang the whole device of putting on the style microscope of just operating this moment, and the setting of filter 4 can place the rubber tube and overturn on style microscope to the interim of device is put in the convenience.

In one embodiment, the device is suitable for use in the egg detection process after in vitro fertilization of mice, and in the egg detection process, fertilized eggs (with the diameter of about 100 um) need to be rapidly selected from a culture solution containing impurities, and continuously transferred into fresh egg washing solution for 2-3 times to be washed so as to remove the impurities attached to embryos. When the diameter of the egg detecting needle is small, fertilized eggs are selected cleanly, but the egg storage quantity is limited, and when the diameter is large, a plurality of impurities are easy to inhale.

Referring to fig. 2 and 3, in this embodiment, the entire glass needle 8 is further thinned, the entire glass needle 8 is used as an egg-detecting needle, the glass needle 8 includes a holding section 9 and a capillary section 10, the holding section 9 is used for being attached to the needle holder 7, one end of the capillary section 10 is connected to the holding section 9, the other end is a needle tip 11, and the needle tip 11 is provided in a tapered transition.

By the arrangement of the structure, the whole glass needle 8 not only can have a smaller needle head structure, but also can store sucked fertilized eggs after the capillary section 10 with the gradually conical shape is gradually enlarged, and has higher use practicability.

In this embodiment, the mouth of the needle tip 11 is rounded and smooth. In addition, the inner diameter of the needle opening can be selected to be 100-120um, and the inner diameter of the capillary section 10 can be selected to be 200-300 um. In particular, in this embodiment, the inner diameter of the needle opening is in particular 110um and the inner diameter of the capillary segment 10 is in particular 250um. In the egg detection process, the front end diameter is small, the rear end diameter is large, the front end of the needle point 11 is provided with the needle point 11 with a gentle and excessive cone, the front end of the needle point 11 can avoid sucking excessive impurities in liquid, the capillary section 10 at the rear end of the needle point 11 can also ensure the storage capacity of fertilized eggs, and the egg detection rate is improved.

Referring to fig. 4 and 5, in one embodiment, the device is suitable for the embryo oviduct transplantation process of mice, and in the embryo oviduct transplantation process, the large diameter of the transplantation needle can lead to large oviduct wound, too large diameter is not easy to enter the oviduct, small diameter is favorable for transplantation, but easy to block, too small diameter is easy to squeeze embryos, embryos and culture solution, and difficult to descend. In addition, the problem of blockage or reflux is easily caused by improper dimensions and angles of insertion of the implantation needle into the fallopian tube.

In this embodiment, the glass needle 8 is further refined as a whole, the glass needle 8 is used specifically as a transplanting needle, and the glass needle 8 includes a holding section 9 and a capillary section 10, wherein the holding section 9 is used for mounting connection with the needle body holder 7. One end of the capillary section 10 is connected with the holding section 9, the other end is provided with a needle point 11, the needle point 11 and the capillary section 10 are arranged in a deflection way, the deflection angle can be selected to be 15-30 degrees, and the deflection length of the needle point 11 can be selected to be 0.5-1mm. Specifically, in this embodiment, the deflection angle is set to 20 degrees, and the deflection length of the needle tip 11 is 1mm.

The needle tip 11 is biased to make the needle tip 11 of the glass needle 8 enter the oviduct and then be parallel to the oviduct, which is beneficial to the subsequent transplanting operation. Meanwhile, the deflection length of the needle tip 11 is set, so that the depth of the glass needle 8 entering the oviduct can be conveniently controlled, the blocking of the needle or the backflow of the oviduct wall can be avoided, and the success rate of transplantation can be improved.

The inner diameter of the capillary segment 10 may be selected to be 100-120um, and in this embodiment, specifically, the inner diameter of the capillary segment 10 is 120um, and in addition, the needle mouth of the needle tip 11 is provided in a round and smooth manner.

By providing the needle tip 11 with a diameter slightly larger than 2-cell embryo (about 100 um), the problems of easy blockage, easy extrusion of embryos, difficult descending of embryos and culture solution can be solved, and the success rate of transplantation can be improved. In addition, the smooth needle tip 11 is advantageous in that damage to fertilized eggs, embryos, oviducts, and the like caused by the needle tip 11 can be avoided.

In one embodiment, the device is integrally provided with two glass needles 8, one of which is the above-mentioned egg-detecting needle and the other of which is the above-mentioned transplanting needle;

The ovum detecting needle is structurally characterized in that the glass needle 8 comprises a holding section 9 and a capillary section 10, the holding section 9 is used for being connected with the needle body fixer 7 in an installation mode, one end of the capillary section 10 is connected with the holding section 9, the other end of the capillary section is a needle point 11, the needle point 11 is in conical transition, and a needle opening of the needle point 11 is round and smooth. Specifically, in this embodiment, the inner diameter of the needle opening is specifically 110um, and the inner diameter of the capillary segment 10 is specifically 250um;

The glass needle 8 comprises a holding section 9 and a capillary section 10, wherein the holding section 9 is used for being connected with the needle body fixer 7 in a mounting way. One end of the capillary section 10 is connected with the holding section 9, the other end is a needle tip 11, the needle tip 11 and the capillary section 10 are arranged in a deflection manner, in the embodiment, the deflection angle is set to 20 degrees, the deflection length of the needle tip 11 is 1mm, the inner diameter of the capillary section 10 is 120um, and in addition, the needle mouth of the needle tip 11 is arranged in a round and smooth manner.

The specific embodiment is as follows:

During egg detection, an egg detection needle is selected to be mounted into a needle body fixer 7 as a glass needle 8, a filter element gun head 1 is held in an oral cavity, the needle body fixer 7 is held by a right hand, the egg detection needle is stretched into in-vitro fertilization culture solution drops under a split microscope, the glass needle 8 is sucked into the needle tip 11 by utilizing oral suction fertilized eggs when the needle tip 11 is close to fertilized eggs, all fertilized eggs in the culture solution are continuously sucked and transferred to a new culture solution, and the repeated suction is repeated and transferred to the new culture solution for 3 times, so that the washing of fertilized eggs (namely egg detection) can be completed.

During transplantation, the transplanting needle is selected to be mounted into the needle body fixer 7 as a glass needle 8, the filter core gun head 1 is held in the oral cavity, meanwhile, the needle body fixer 7 is held in the right hand, 2-3 small bubbles are alternately sucked by the oral cavity at intervals of 2-3mm under a split microscope, then 2-cell embryos are sucked into the glass needle 8 one by one in the culture solution until the last embryo is positioned above the deflection position by utilizing oral cavity suction, the interval between the embryos is as small as possible, the transplanting needle is pricked into the oviduct along the direction from the ovary to the uterus through an opening on the oviduct at the front end of the expanded part of the mouse, when the deflection position contacts the oviduct, the extension is stopped, the needle tip 11 of the transplanting needle is parallel to the oviduct, and the transplanting needle can be drawn out after the small bubbles are observed to be generated after the embryo in the transplanting needle is injected into the oviduct by the oral cavity by gentle blowing.

It should be noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present utility model, and not for limiting the same, and although the present utility model has been described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that the technical solution described in the above-mentioned embodiments may be modified or some technical features may be equivalently replaced, and these modifications or substitutions do not make the essence of the corresponding technical solution deviate from the spirit and scope of the technical solution of the embodiments of the present utility model.

Claims (9)

1. An egg testing and transplanting device, comprising:

a rubber tube;

The suction nozzle is connected with one end of the rubber tube;

the filter element gun head is connected to the suction nozzle;

The needle body fixer is connected to the other end of the rubber tube;

A glass needle provided on the needle body holder, and

And the cotton plug is arranged in the rubber tube and is contacted with the end part of the glass needle.

2. An egg and graft device as in claim 1, wherein a filter is attached to the middle portion of the rubber tube.

3. An egg and graft device as in claim 1, wherein the glass needle comprises:

a holding section for mounting connection with the needle holder, and

And one end of the capillary section is connected with the holding section, the other end of the capillary section is a needle point, and the needle point is in conical transition.

4. An egg and transplant device as claimed in claim 3 wherein the mouth of the needle tip is round and smooth.

5. An egg and graft device as in claim 4, wherein the needle port has an inner diameter of 100-120um and the capillary section has an inner diameter of 200-300 um.

6. An egg and graft device as in claim 1, wherein the glass needle comprises:

a holding section for mounting connection with the needle holder, and

The capillary section, the one end of capillary section with hold the section and be connected, the other end is the needle point, the needle point with capillary section is the deflection setting, and deflection angle is 15-30 degrees.

7. An egg and graft device as in claim 6, wherein the capillary section has an inner diameter of 100-120um.

8. An egg and graft device as in claim 6, wherein the tip has a deflection length of 1mm.

9. An egg and transplant device as claimed in claim 6 wherein the mouth of the needle tip is round and smooth.