CN207503515U - Human gastrointestinal tract in-vitro simulated digester - Google Patents

Abstract

The utility model discloses an in vitro simulated digester for the human gastrointestinal tract, which comprises two reaction tanks, a middle connection mechanism, two end connection mechanisms, a controller, a material peristaltic pump and a pressure peristaltic pump; A silicone tube is provided, and the two ends of the silicone tube are respectively sealed and connected to the two ends of the reaction tank, forming an independent interlayer cavity with the inner wall of the reaction tank, and the wall of the reaction tank is provided with a pressure inlet and a pressure outlet to communicate with the interlayer cavity; One end opening is connected through the middle connection mechanism, and the other openings of the two reaction tanks are respectively connected to an end connection mechanism; the end connection mechanism is provided with a material port; the controller is connected to control the material peristaltic pump and the pressure peristaltic pump; the material peristaltic pump is connected to the material port ; The pressure peristaltic pump is connected to the pressure inlet or pressure outlet. The utility model simulates reality and has good reproducibility, and is suitable for research in technical fields such as microbiology, pharmacodynamics, toxicology and nutrition.

Description

An in vitro simulated digester of human gastrointestinal tract

technical field

The utility model belongs to the technical fields of bionics, microbiology, pharmacodynamics, toxicology, nutrition and analysis and testing. The utility model relates to an in vitro simulator of the human gastrointestinal tract, in particular to a device for simulating the digestion and absorption of food in different intestinal cavities of the human gastrointestinal tract under external conditions.

Background technique

The digestive system of the human body is mainly composed of the digestive tract and digestive glands. The alimentary canal starts from the mouth, followed by the pharynx, esophagus, stomach, small intestine, and large intestine; the digestive glands include salivary glands, pancreas, liver, gastric glands, and intestinal glands. The main function of the digestive system is to ingest, digest food, absorb nutrients, and eliminate food residues. The digestion and absorption process of food by the human body is extremely complicated. The digestion process mainly refers to the decomposition of food into small molecules that can be absorbed in the digestive tract; the absorption process is the process in which the substances in the digestive tract enter the blood and lymph through the mucous membrane of the digestive tract. In the digestive process of the human body, the two main processes of digestion and absorption occur almost simultaneously. At present, the human digestive system has become a research hotspot in the fields of microbiology, pharmacodynamics, toxicology, and nutrition. However, due to the complexity of the human digestive system, the limitations of scientific research techniques, and the ethical constraints involved in the in vivo research process, the study of the human digestive system has always been difficult. Therefore, the academic community urgently needs a set of flexible, accurate, and repeatable in vitro models for simulating physiological processes such as human digestion and absorption.

In recent years, human and animal digestive tract simulation devices in vitro have been continuously promoted. These simulation devices have been widely used in the research fields of microbiology, pharmacodynamics, toxicology, human and animal nutrition, and are gradually accepted by the academic community. The device can even serve as a production facility for certain active substances that require a specific intestinal environment or activation of intestinal bacteria. These published in vitro digestive tract simulation devices all have the following similar design concepts: the core components of the simulated stomach, small intestine and other digestive tract parts are closed reaction vessels with different numbers, and different digestive juices, Adjust the appropriate pH value to roughly simulate the environment in the digestive tract of humans and animals, and each simulation device is equipped with peristaltic pumps, magnetic stirrers, temperature sensors and other moving parts.

For human in vitro gastrointestinal tract models, the design types can be roughly divided into four types: static single-chamber model, dynamic single-chamber model, dynamic double-chamber model, and dynamic multi-chamber model. The representative of the static single-chamber model is the gastrointestinal digestive system developed by Dutch scientist A.G.Oomen et al. in 2002. The system only includes a reaction tank, and the digestive juice only includes saliva, gastric juice, pancreatic juice and bile. Although the digestive system is widely used, its static limitations cannot simulate the complex physiological, biochemical and physicochemical characteristics of the human digestive system. At present, the most advanced and accurate in vitro digestion model of the human gastrointestinal tract is the TIM system designed by TNO in the Netherlands. This system is a dynamic and multi-chamber system controlled by a computer. The control system controls the process of food chewing, digestion, absorption and gastric emptying, and accurately reproduces the digestive process of the human gastrointestinal tract. In my country, the research on the in vitro simulated digestive system of the human gastrointestinal tract is relatively lagging behind. At present, only a few institutions conduct research on devices and methods. A cultured device that provides and maintains a certain rate of oscillation, relatively constant temperature and humidity, to achieve the effect of simulating the human intestinal environment. The utility model patent authorization number CN201344874Y discloses an animal simulated digester. By injecting the digestive juice of different digestive tract segments such as the stomach and duodenum into the dialysis tube and correspondingly changing the buffer solution passed into the glass tube, it can be realized in vitro. Under the conditions, the situation that the feed is digested and absorbed in each segment of the animal gastrointestinal tract is simulated. Invention patent authorization number CN103740589A discloses a human gastrointestinal bionic system and a simulation experiment method based on the system. The system can truly simulate the digestion and absorption of food in the human gastrointestinal tract under in vitro conditions through intelligent control. The invention patent authorization number CN104851346A discloses a modular animal digestive tract in vitro simulation system and a human intestinal simulation method thereof. The system simulates five digestive tracts including stomach, small intestine, ascending colon, transverse colon, and descending colon, and integrates multiple functional modules into the framework. The reaction tank and functional modules are controlled by an intelligent control system to realize automatic sampling and automatic storage of samples. parameter.

However, the above-mentioned in vitro simulation device systems disclosed above all have their own defects. For example, the devices basically use a magnetic stirrer to simulate the peristaltic function of the gastrointestinal tract. In the gastrointestinal tract simulation experiment, the magnetic stirrer needs to be able to work continuously for at least 200 hours. , but the general magnetic stirrer cannot work continuously for more than 10 hours. And with the advancement of sensors and precision control technology, more controllable components or collected information will inevitably appear in future models. The design of existing devices inevitably encounters structural adjustment or modification, and its intelligent control system must be improved accordingly with the deepening of research.

Utility model content

In view of the above problems, the purpose of this utility model is to provide an in vitro simulated digester for the human gastrointestinal tract, which can truly simulate the digestion of food in different intestinal lumens of the human gastrointestinal tract under in vitro conditions, Absorption situation.

In order to achieve the above object, the utility model adopts the following technical solutions:

An in vitro simulated digester for the human gastrointestinal tract, the in vitro human digestive tract simulator includes two reaction tanks, a middle connection mechanism, two end connection mechanisms, a controller, a material peristaltic pump and a pressure peristaltic pump;

Both the middle connection mechanism and the end connection mechanism include a cavity that can accommodate liquid;

Both ends of the reaction tank are open; the interior of the reaction tank is provided with a silicone tube, and the two ends of the silicone tube are respectively sealed and connected to the two ends of the reaction tank, and the outer wall of the silicone tube and the inner wall of the reaction tank form an independent interlayer space cavity, the inside of the silicone tube communicates with the openings at both ends of the reaction tank; the wall of the reaction tank is provided with a pressure inlet and a pressure outlet, and both the pressure inlet and the pressure outlet are connected to the interlayer cavity;

Openings at one end of the two reaction tanks communicate through a cavity in the middle connection mechanism, and the other openings of the two reaction tanks communicate with a cavity of the end connection mechanism respectively;

A material port is provided on the terminal connection mechanism, and the material port communicates with the cavity of the terminal connection mechanism;

The controller is connected to control the material peristaltic pump and the pressure peristaltic pump; the material peristaltic pump is connected to the material port; the pressure peristaltic pump is connected to the pressure inlet or pressure outlet.

The constant temperature function can be realized by passing constant flow and constant temperature water into the interlayer cavity.

Further, the human digestive tract in vitro simulation unit is provided with a connector, and the connector communicates with the pressure inlet or pressure outlet. The detection of the liquid level in the tank and the measurement of the volume of the interlayer cavity in the tank can be realized by installing a non-contact liquid level gauge and a connector.

Further, at least one of the terminal connection mechanisms is provided with an acid-base solution inlet, and the acid-base solution inlet communicates with the cavity of the terminal connection mechanism.

Further, the middle connection mechanism is provided with a pH meter port, and the pH meter port communicates with the cavity of the middle connection mechanism; the pH meter port is provided with a pH meter, and the pH meter is connected to the controller; The controller is connected with an acid-base liquid peristaltic pump, and the acid-base liquid peristaltic pump is connected to the acid-base liquid inlet. Set the target pH value through the controller. If the pH is lower than the minimum or maximum value, start the acid and alkali peristaltic pump, inject diluted alkali or acid into the simulated reaction tank, and stop the acid and alkali peristaltic pump when the target value is reached. .

Further, the openings at both ends of the reaction tank are grinding ports, which communicate with the middle connection mechanism or the end connection mechanism through flanges.

Further, the two ends of the silicone tube are turned out at the opening of the reaction tank.

Further, the reaction tank is a cylindrical tank.

Further, the reaction tank is made of high temperature and high pressure resistant glass material.

Further, the in vitro simulated digester of the human gastrointestinal tract also includes a temperature regulation system, and the temperature regulation system includes a sealed constant temperature water tank and a temperature sensor; the constant temperature water tank is controlled by the controller, and the controller is connected to the The temperature sensor is arranged in the constant temperature water tank. Set the target value, maximum value, and minimum value of the liquid temperature in the constant temperature water tank. The temperature sensor detects the liquid temperature in the constant temperature water tank. If it is lower than the minimum value, the controller controls the constant temperature water tank to heat until it reaches the target value; if the temperature is higher than the specified value When the maximum value is reached, the control pressure peristaltic pump stops injecting hot water into the interlayer.

The utility model can change the internal volume of the silicone tube by supplying gas or liquid to the interlayer cavity, and the peristalsis of the gastrointestinal tract can be simulated through the continuous extrusion and contraction process of the silicone tube.

The utility model has the following advantages:

In the utility model, the silicone tube with good telescopic performance is used to replace the traditional magnetic stirrer to simulate the peristaltic function of the gastrointestinal tract, which can overcome the defect that the magnetic stirrer cannot work for a long time, and the shrinkage and expansion process of the silicone tube is closer to the gastrointestinal tract. Creeping state, the simulation is more realistic.

Description of drawings

Fig. 1 is a schematic diagram of the mechanism of the in vitro simulated digestion unit of the human gastrointestinal tract of the present invention.

Among them, 1 is the reaction tank, 2 is the silicone tube, 3 is the interlayer cavity, 4 is the end connection mechanism, 5 is the middle connection mechanism, 6 is the pressure inlet, 7 is the pressure outlet, 8 is the material port, and 9 is the acid and alkali liquid Import, 10 is the pH meter port.

Detailed ways

As shown in Figure 1,

An in vitro simulated digester for the human gastrointestinal tract, the human digestive tract in vitro simulator includes two reaction tanks (1), a middle connection mechanism (5), two end connection mechanisms (4), a controller, and a material peristaltic pump and pressure peristaltic pumps;

Both the middle connection mechanism (5) and the end connection mechanism (4) include a cavity that can accommodate liquid;

Both ends of the reaction tank (1) are open; the inside of the reaction tank (1) is provided with a silicone tube (2), and the two ends of the silicone tube (2) are respectively sealed and connected to the two ends of the reaction tank (1). The outer wall of the silicone tube (2) and the inner wall of the reaction tank (1) form an independent interlayer cavity (3), and the inside of the silicone tube (2) communicates with the openings at both ends of the reaction tank (1); The wall of the tank (1) is provided with a pressure inlet (6) and a pressure outlet (7), and the pressure inlet (6) and the pressure outlet (7) are both connected to the interlayer cavity (3);

One end openings of the two reaction tanks (1) communicate through the cavity in the middle connection mechanism (5), and the other openings of the two reaction tanks (1) communicate with one of the end connection mechanisms (4) respectively. ) cavity;

The terminal connection mechanism (4) is provided with a material port (8), and the material port (8) communicates with the cavity of the terminal connection mechanism (4);

The controller is connected to control the material peristaltic pump and the pressure peristaltic pump; the material peristaltic pump is connected to the material port (8); the pressure peristaltic pump is connected to the pressure inlet (6) or pressure outlet (7 ).

The constant temperature function can be realized by passing constant flow and constant temperature water into the interlayer cavity (3).

Further, the human digestive tract in vitro simulation unit is provided with a connector, and the connector communicates with the pressure inlet (6) or pressure outlet (7). The detection of the liquid level in the tank and the measurement of the volume of the interlayer cavity (3) in the tank can be realized by installing a non-contact liquid level gauge and a connector.

Further, at least one of the terminal connection mechanisms (4) is provided with an acid-base solution inlet (9), and the acid-base solution inlet (9) communicates with the cavity of the terminal connection mechanism (4).

Further, the middle connecting mechanism (5) is provided with a pH meter port (10), and the pH meter port (10) communicates with the cavity of the middle connecting mechanism (5); the pH meter port (10) A pH meter is arranged in the center, and the pH meter is connected to a controller; the controller is connected to an acid-base liquid peristaltic pump, and the acid-base liquid peristaltic pump is connected to the acid-base liquid inlet (9). Set the target pH value through the controller. If the pH is lower than the minimum or maximum value, start the acid-base peristaltic pump, inject diluted lye or acid into the simulated reaction tank (1), and stop the acid-base when the target value is reached. liquid peristaltic pump.

Further, the openings at both ends of the reaction tank (1) are grinding ports, which communicate with the middle connection mechanism (5) or the end connection mechanism (4) through flanges.

Further, the two ends of the silicone tube (2) are turned out at the opening of the reaction tank (1).

Further, the reaction tank (1) is a cylindrical tank.

Further, the reaction tank (1) is made of high temperature and high pressure resistant glass material.

Further, the in vitro simulated digester of the human gastrointestinal tract also includes a temperature regulation system, and the temperature regulation system includes a sealed constant temperature water tank and a temperature sensor; the constant temperature water tank is controlled by the controller, and the controller is connected to the The temperature sensor is arranged in the constant temperature water tank. Set the target value, maximum value, and minimum value of the liquid temperature in the constant temperature water tank. The temperature sensor detects the liquid temperature in the constant temperature water tank. If it is lower than the minimum value, the controller controls the constant temperature water tank to heat until it reaches the target value; if the temperature is higher than the specified value When the maximum value is reached, the control pressure peristaltic pump stops injecting hot water into the interlayer.

When the utility model is used to simulate the gastric digestion process of the human body, food samples and gastric simulated digestive juice are put into the reaction tank (1) through the material port (8), and the constant temperature water bath is opened to inject water into the interlayer cavity (3), and the silicone tube (2) The pressure of the constant temperature water in the interlayer cavity (3) shrinks, and the food sample enters the other end from one end of the reaction tank (1). By adjusting the pressure in the interlayer cavity (3), the food sample reciprocates from the cavity of the reaction tank (1) silicone tube (2), and this process realizes the simulation of gastric peristalsis. During this operation, a pH meter can be inserted into the cavity of the middle connecting mechanism (5) to monitor the pH value of the sample at all times. Set the target pH value through the system controller, if the pH in the cavity is lower than the minimum or maximum value, start the acid and alkali peristaltic pump, and inject diluted alkali into the acid and alkali inlet (9) in the simulated reaction tank (1) Or acid, after reaching the target value, stop the acid and alkali peristaltic pump. In this process, the temperature of the water in the interlayer cavity (3) is controlled by the sealed constant temperature water tank, and then the temperature of the food sample is controlled, so as to simulate the temperature of the gastrointestinal tract of the human body to the greatest extent. After the food sample fully reacts with the gastric reaction liquid in the reaction tank (1), the pressure supply is stopped, thereby completing the simulated digestion process of the stomach.

Claims (8)

1. An in vitro simulated digester for the human gastrointestinal tract, characterized in that it comprises two reaction tanks, a middle connection mechanism, two end connection mechanisms, a controller, a material peristaltic pump and a pressure peristaltic pump; Both the middle connection mechanism and the end connection mechanism include a cavity that can accommodate liquid; Both ends of the reaction tank are open; the interior of the reaction tank is provided with a silicone tube, and the two ends of the silicone tube are respectively sealed and connected to the two ends of the reaction tank, and the outer wall of the silicone tube and the inner wall of the reaction tank form an independent interlayer space cavity, the inside of the silicone tube communicates with the openings at both ends of the reaction tank; the wall of the reaction tank is provided with a pressure inlet and a pressure outlet, and both the pressure inlet and the pressure outlet are connected to the interlayer cavity; Openings at one end of the two reaction tanks communicate through a cavity in the middle connection mechanism, and the other openings of the two reaction tanks communicate with a cavity of the end connection mechanism respectively; A material port is provided on the terminal connection mechanism, and the material port communicates with the cavity of the terminal connection mechanism; The controller is connected to control the material peristaltic pump and the pressure peristaltic pump; the material peristaltic pump is connected to the material port; the pressure peristaltic pump is connected to the pressure inlet or pressure outlet. 2 . The digester according to claim 1 , wherein at least one of the end connection mechanisms is provided with an acid-base inlet, and the acid-base inlet communicates with the cavity of the end connection mechanism. 3. The digester according to claim 1, characterized in that, the middle connection mechanism is provided with a pH meter port, and the pH meter port communicates with the cavity of the middle connection mechanism; the pH meter mouth is provided with A pH meter, the pH meter is connected to a controller; the controller is connected to an acid and alkali liquid peristaltic pump, and the acid and alkali liquid peristaltic pump is connected to the acid and alkali liquid inlet. 4. The digester according to claim 1, wherein the openings at both ends of the reaction tank are ground openings, which communicate with the middle connection mechanism or the end connection mechanism through flanges. 5. The digester according to claim 1, characterized in that, the two ends of the silicone tube are turned outward at the opening of the reaction tank. 6. The digester according to claim 1, characterized in that, the reaction tank is a cylindrical tank. 7. The digester according to claim 1, wherein the reaction tank is made of high temperature and high pressure resistant glass material. 8. The digester according to claim 1, characterized in that, the human gastrointestinal tract simulated digester in vitro also includes a temperature regulation system, and the temperature regulation system includes a sealed constant temperature water tank and a temperature sensor; the constant temperature water tank Controlled by the controller, the controller is connected to the temperature sensor, and the temperature sensor is arranged in the constant temperature water tank.