CN110632963A - A fully automatic program-controlled simulated bionic gastrointestinal digestion device - Google Patents

Abstract

The invention discloses a fully automatic program-controlled simulated bionic gastrointestinal digestion device, which belongs to the technical field of food science experiments. The device includes stepless variable speed motor, transmission equipment, soft reaction tank, fixture, peristaltic pump, valve, peristaltic roller, heat-insulated hollow box, acid-base solution conduit, heating system, plexiglass panel, enzyme solution conduit, sealing cover , Computer program-controlled terminal, pH/temperature probe. The invention provides a fully automatic bionic gastrointestinal simulated digestion device for a human body capable of real-time monitoring and convenient sampling.

Description

A fully automatic program-controlled simulated bionic gastrointestinal digestion device

technical field

The invention belongs to the technical field of food science experiments, and in particular relates to a fully automatic program-controlled simulated bionic gastrointestinal digestion device.

Background technique

In vitro digestion models have been widely used in various fields such as medicine, nutrition, toxicology, and physiology, as well as in various industries such as food, health care products, and pharmaceuticals. It is used to study the physical, chemical and biological changes of food/drugs in the process of human digestion. Especially in the field of food science, the in vitro simulated digestion model can be used to study the interaction of food during digestion, the structural changes of food at different scales during digestion, the utilization efficiency of nutrients, the release of functional components, and the glycemic index of carbohydrates. Evaluation etc. At present, the in vitro digestion model of human body has gradually become more complicated, and the recognition of the use of in vitro digestion model for related research at home and abroad is getting higher and higher. However, compared with in vivo conditions, the existing gastrointestinal tract models are still relatively simple. There is a gap in the establishment and precise hormone control, which leads to poor correlation between most of the experimental data and the results of in vivo experiments. The main reason is that the pH and digestive enzymes in the digestion process cannot be precisely controlled , temperature and creep etc.

Contents of the invention

In order to solve the problems that the current gastrointestinal simulated digestion equipment cannot accurately control temperature, pH and peristalsis, the present invention provides a fully automatic program-controlled simulated bionic gastrointestinal digestion device based on the existing research foundation of human gastrointestinal simulated digestion, so as to realize Fully automatic program-controlled human bionic gastrointestinal digestion device.

In order to achieve the above object, the technical solution of the present invention is as follows: a fully automatic program-controlled simulated bionic gastrointestinal digestion device, the device includes a stepless variable speed motor, a transmission device, a belt, a soft reaction tank, a fixing device, a peristaltic pump, a valve , Peristaltic rollers, heat-insulated hollow box, acid-base solution conduit, heating system, plexiglass panel, enzyme solution conduit, sealing cover, computer program-controlled terminal, pH/temperature probe. The computer program-controlled terminal and the peristaltic pump are arranged on the left side of the plexiglass panel, the plexiglass panel is located above the heat-insulating hollow box, the steplessly variable speed motor is arranged at the bottom of the heat-insulating hollow box, the heating system, The transmission equipment is set in the heat-insulating hollow box, and the transmission equipment is connected with the stepless variable speed motor through a belt; the plexiglass panel and the upper part of the heat-insulation hollow box are provided with holes, and the upper end of the soft reaction tank passes through the hole, and passes through the fixing device fixed, the sealing cap is set on the upper port of the soft reaction tank, the valve is set at the bottom of the soft reaction tank, and the pH/temperature probe is embedded in the inner side of the soft reaction tank. The peristaltic rollers are embedded on the belt and distributed around the soft reaction tank, and the bottom of the peristaltic rollers and the heat-insulating hollow box are fixed by screws. The outlet of the acid-base solution conduit and the outlet of the enzyme solution conduit are connected with the upper port of the soft reaction tank, and the enzyme solution conduit and the acid-base solution conduit are connected to the peristaltic pump by roller pressure.

The peristaltic pump, the inorganic variable speed motor, the heating system, and the pH/temperature probe are respectively connected to a computer program-controlled terminal.

Further, the soft reaction tank is made of silica gel, the middle of the soft reaction tank is circular, the two ends are tapered, and both ends are open.

Further, the heating system includes heating equipment and a temperature sensing probe, the heating equipment is fixed on the wall of the heat-insulating hollow box by screws, and the temperature sensing probe is embedded in the inner wall of the heat-insulating hollow box.

Compared with the prior art, the present invention has the following beneficial effects: In the present invention, silica gel material with better stretchability is used as the wall material of the reactor, and temperature and pH sensor probes are embedded on the inner wall of the silica gel, compared with traditional magnetic Stirring and conventional silicone wall materials without probes can more realistically simulate the peristaltic state and more realistically control the key parameters in the simulated digestion process. Compared with the traditional gastrointestinal simulated digestion equipment, the equipment can realize precise and convenient regulation of temperature, gastrointestinal motility rate, pH and enzyme concentration through the computer program-controlled terminal, so as to realize precise regulation of the entire simulated digestion process. The device has the characteristics of real-time monitoring and easy operation.

Description of drawings

Fig. 1 is a schematic diagram of the human gastrointestinal tract in vitro simulated digestion unit mechanism of the present invention, (A) is a front view, (B) is a rear view, (C) is a top view, and (D) is a perspective view.

Among them: 1 stepless variable speed motor, 2 transmission equipment, 3 soft reaction tank, 4 fixing device, 5 peristaltic pump, 6 valve, 7 peristaltic roller, 8 temperature-insulated inner wall, 9 stainless steel shell, 10 acid-base solution conduit, 11 heating Equipment, 12 plexiglass panels, 13 enzyme solution conduits, 14 sealing caps, 15 computer program-controlled terminals.

Detailed ways

As shown in Figure 1, it is a schematic diagram of the human gastrointestinal tract in vitro simulated digestion unit mechanism of the present invention. The device includes a stepless variable speed motor 1, a transmission device 2, a belt, a soft reaction tank 3, a fixing device 4, a peristaltic pump 5, and a valve 6. Peristaltic roller 7, heat-insulated hollow box, acid-base solution conduit 10, heating system, plexiglass panel 12, enzyme solution conduit 13, sealing cover 14, computer program-controlled terminal 15, pH/temperature probe. The computer program-controlled terminal 15 and the peristaltic pump 5 are arranged on the plexiglass panel 12; the plexiglass panel 12 is located above the heat-insulating hollow box, and the continuously variable speed motor 1 is arranged at the bottom of the heat-insulated hollow box, and the The heating system and the transmission equipment 2 are set in the heat-insulating hollow box, and the transmission equipment 2 is connected with the stepless variable speed motor 1 through a belt; the heat-insulation hollow box is composed of two layers inside and outside, including a temperature-insulating inner wall 8 and a stainless steel shell 9 . Those skilled in the art only need to set holes on the plexiglass panel 12 and the upper part of the heat-insulating hollow box, pass the upper end of the soft reaction tank 3 through the hole, and fix it through the fixing device 4, which is a detachable buckle , can realize the fast and thorough cleaning of the soft reaction tank. Those skilled in the art only need to pour the material to be simulated into the soft reaction tank 3 from the upper port of the soft reaction tank 3 to simulate the digestion process, and the sealing cover 14 is arranged on the upper port of the soft reaction tank 3, The valve 6 is arranged at the bottom of the soft reaction tank 3 , and the valve 6 is also a detachable buckle, which can realize quick and thorough cleaning of the soft reaction tank 3 . The pH/temperature probe is embedded in the inner side of the soft reaction tank 3 and connected with the computer program control terminal 15 to realize real-time monitoring of the temperature/pH in the soft reaction tank 3 . The peristaltic rollers 7 are embedded on the belt and distributed around the soft reaction tank 3. The bottom of the peristaltic rollers 7 and the heat-insulating hollow box are fixed by screws. During the rotation of the continuously variable speed motor 1, the peristaltic rollers 7 Regularly rotate and squeeze the soft reaction tank 3 to simulate gastrointestinal peristalsis. Those skilled in the art only need to connect the outlet of the acid-base solution conduit 10 and the outlet of the enzyme solution conduit 13 with the upper port of the soft reaction tank 3, and the enzyme solution conduit 13, the acid-base solution conduit 10 and the peristaltic pump 5 Rolling connection makes the acid-base liquid and enzyme liquid pumped into the soft reaction tank 3 .

The peristaltic pump 5 is connected to the computer program-controlled terminal 15, and through the control instructions of the computer program-controlled terminal 15, the signal is fed back to the enzyme solution conduit 13 and the acid-base solution conduit 10, so as to directly realize the precise control of the amount of acid-base and enzyme added.

The inorganic speed-variable motor 1 is connected to the computer program-controlled terminal 15, and the rotation rate of the peristaltic roller 7 is adjusted by issuing instructions from the computer program-controlled terminal 15, so as to realize different extrusion force simulations of the stomach and small intestine.

The heating system is connected with the computer program-controlled terminal 15 to realize real-time monitoring of the temperature/pH in the soft reaction tank 3 .

The soft reaction tank 3 is made of silica gel material, the middle of the soft reaction tank 3 is circular, the two ends are tapered, and both ends are open. The inside of the soft reaction tank 3 can hold liquid, semi-fluid and solid digestate.

The heating system includes a heating device 11 and a temperature sensing probe, the heating device 11 is fixed on the wall of the insulating hollow box by screws, and the temperature sensing probe is embedded in the inner wall of the insulating hollow box. By controlling the temperature of the heat-insulated hollow box, the temperature in the soft reaction tank 3 is kept constant. When the temperature is lower than the set temperature, the heating system starts automatically, and when the temperature is higher than the set temperature, the hot air heating system stops working.

The working process of simulating the gastrointestinal digestion of the human body in the present invention is as follows: firstly preheat the insulating hollow box to the set temperature, then open the sealing cover 14 on the upper end of the soft reaction tank 3, put the sample into the soft reaction tank 3, Turn on the peristaltic pump 5, pump the acid solution and gastric digestive enzyme solution into the soft reaction tank 3, and then start the inorganic variable speed motor 1 to simulate gastric peristalsis. During this operation, the multiple pH/temperature sensors embedded in the inner wall of the soft reaction tank 3 monitor the digestive fluid environment in real time. If the pH of the digestive solution is lower than the minimum or maximum value, start the acid-base peristaltic pump, inject diluted lye or acid into the soft reaction tank 3, and stop the acid-base peristaltic pump after reaching the target value. In this process, the temperature in the soft reaction tank 3 is also controlled through the hollow heat insulation box and the heating system, so as to simulate the temperature of the human gastrointestinal tract to the greatest extent. After the sample fully reacts with the gastric reaction solution in the soft reaction tank 3, the pH of the solution is adjusted to the digestive environment of the small intestine through the peristaltic pump 5, and then a certain amount of digestive enzymes such as pancreatic enzymes are pumped into the small intestine through the peristaltic pump 5 to adjust the rotation speed of the roller , simulating the stages of digestion in the small intestine. During the whole simulated reaction process, at any time point, the sealing cover on the upper end of the soft reaction tank can be opened for sampling and analysis, so as to complete the analysis of the physicochemical and biological characteristics of the food during the simulated digestion process.

Claims (3)

1. A full-automatic program-controlled simulated bionic gastrointestinal digestion device is characterized in that said equipment comprises stepless variable speed motor (1), transmission equipment (2), belt, soft reaction tank (3), fixture ( 4), peristaltic pump (5), valve (6), peristaltic roller (7), heat-insulated hollow box, acid-base solution conduit (10), heating system, plexiglass panel (12), enzyme solution conduit (13), Sealing cap (14), computer program-controlled terminal (15), pH/temperature probe. The computer program-controlled terminal (15) and the peristaltic pump (5) are arranged on the left side of the plexiglass panel (12), the plexiglass panel (12) is positioned above the heat-insulating hollow box, and the continuously variable speed motor (1 ) is arranged at the bottom of the heat-insulating hollow box, the heating system and transmission equipment (2) are arranged in the heat-insulation hollow box, and the transmission equipment (2) is connected with the continuously variable speed motor (1) through a belt; the organic The glass panel (12) and the upper part of the heat-insulating hollow box are provided with holes, and the upper end of the soft reaction tank (3) passes through the hole and is fixed by the fixing device (4). The sealing cover (14) is arranged on the soft reaction tank (3). ), the valve (6) is arranged at the bottom of the soft reaction tank (3), and the pH/temperature probe is embedded in the inner side of the soft reaction tank (3). The peristaltic rollers (7) are embedded on the belt and distributed around the soft reaction tank (3), and the bottom of the peristaltic rollers (7) and the heat-insulating hollow box are fixed by screws. The outlet of the acid-base solution conduit (10) and the outlet of the enzyme solution conduit (13) are communicated with the upper port of the soft reaction tank (3), and the enzyme solution conduit (13), the acid-base solution conduit (10) and the peristaltic The pump (5) is roller-pressed. The peristaltic pump (5), the inorganic variable-speed motor (1), the heating system, and the pH/temperature probe are respectively connected to a computer program-controlled terminal (15). 2. The bionic gastrointestinal simulation digestion device according to claim 1, wherein the soft reaction tank (3) is made of silica gel, the middle of the soft reaction tank (3) is circular, and the two ends Tapered and open at both ends. 3. The bionic gastrointestinal simulation digestion device according to claim 1, characterized in that the heating system includes a heating device (11) and a temperature sensing probe, and the heating device (11) is fixed to the bottom of the heat-insulating hollow box by screws. On the wall, the temperature sensing probe is embedded in the inner wall of the heat-insulating hollow box.

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