CN206411134U - The repeatable vapour-pressure type ELISA sampling devices utilized - Google Patents

Abstract

The utility model discloses a kind of repeatable vapour-pressure type ELISA sampling devices utilized, it is made up of air pump, multiple loading pipes, ventilation insert row, push rod and outrigger；The front end of loading pipe is embedded in the hollow hole of outrigger.The loading pipe includes external sleeve pipe, piston-in-sleeve, pullover base, check valve sheet；Piston-in-sleeve top is pressure chamber；The pressure chamber of each loading pipe is connected with air pump, is the accommodation space that loading liquid is treated for storing below check valve sheet.External its lower end is sealed by pullover base.By the present apparatus, loading while realizing the quantity of fluid such as same content shortens loading time, at utmost ensures that the reaction time per hole is consistent, can greatly improve conventional efficient and accuracy.It is with low cost and the utility model makes simple, facilitate durable, it is adaptable to research application and business promotion.

Description

Reusable pneumatic ELISA sample loading device

technical field

The utility model relates to a device used in ELISA during scientific research, in particular to a reusable pneumatic ELISA sample loading device.

Background technique

Enzyme-linked immunosorbent assay (ELISA), one of the common molecular biology experiments, is widely used in schools and scientific research institutions. The reaction is carried out on the solid phase surface. This technology can be used to detect macromolecular antigens and specific antibodies. It has the advantages of rapidity, sensitivity, simplicity, and easy standardization of carriers. Commonly used ELISA kits are mostly 96-well plates. When a large sample is used for the test, it takes a long time from the first well of loading the sample to the last well, the error increases, and the reaction time of each well cannot be guaranteed. Consistent, which greatly interfered with the experimental results; except for the standard and sample contents added to each well, the remaining primary antibody working solution, enzyme-labeled antibody working solution, substrate working solution and final stop solution were all Equal amount of equal content liquid per well. Therefore, this device is designed to realize simultaneous addition of the same amount of liquid in the same content, which greatly shortens the sample loading time, is more conducive to reducing the experimental error caused by human operation, and plays a powerful role in improving the efficiency and accuracy of the experiment. .

Contents of the invention

The purpose of the utility model is to provide a reusable pneumatic ELISA sample loading device for the problems of instability, long time consumption and waste of resources in the existing operation.

The purpose of this utility model is achieved by the following technical solutions: a reusable pneumatic ELISA sample loading device, the device is made up of ventilating inserting row, air pump, multiple sample loading tubes, push rod and outer frame; The sample loading tube includes an external casing and a piston inside the casing; the upper end of the external casing is sealed by a top cover, the upper part is cylindrical, and the lower part is conical. The upper part of the piston in the casing is a pressure chamber; two cannulae and two sleeves are arranged around the pressure chamber, both of which are connected with the pressure chamber, and the cannula and sleeve are relatively distributed in position; Inserted into the casing of the adjacent sample loading tube, multiple sample loading tubes are connected to each other through the cannula and the sleeve to form a sample matrix, the unconnected cannula or sleeve at the side is connected or sealed with the ventilation insertion row, The row is connected with the air pump. A one-way valve plate is installed on the lower part of the external casing, and the accommodating space for storing the liquid to be loaded is under the one-way valve plate. The lower end of the external casing is sealed through the socket base. There are multiple hollow holes on the outer frame, and the hollow holes are arranged in a matrix, which corresponds to the hole distribution of the 96-well plate; the front part of the sample loading tube is embedded in the hollow holes. The lower end of the push rod is connected with the piston in the sleeve, and the upper end passes through the top cover and extends to the outside of the outer sleeve. The push rod and the piston in the casing are provided with a vertical through hole communicating with each other, and the upper end of the vertical through hole is sealed by a sealing cover.

Further, the ventilation strip includes a main pipe and a plurality of branch pipes arranged in parallel on one side of the main pipe. The branch pipes communicate with the main pipe and are perpendicular to the main pipe;

The beneficial effects of the utility model are: integrating and redesigning the operation method of the ELISA experiment for the current scientific research, and using the current scientific and technological achievements for assembly, which can improve the efficiency and consistency in the scientific research process, and save costs and reduce resources. Waste, suitable for various colleges and scientific research institutions to carry out experimental operations, easy to promote.

Description of drawings

Fig. 1 is the structural representation of sample loading tube;

Figure 2 is a schematic diagram of the distribution of intubation tubes and sleeves around the sample loading tube;

Figure 3 is a schematic diagram of the connection between the sample loading tube and the cannula through the intubation tube

Fig. 4 is a sample loading schematic diagram;

Figure 5 is a schematic diagram of the ventilation insertion row;

In the figure, the external sleeve 1, the piston inside the sleeve 2, the push rod 3, the socket base 4, the one-way valve plate 5, the liquid to be loaded 6, the ventilation insertion row 7, the air pump 8, the sample hole 9 on the 96-well plate, Outer frame 10, sealing cover 11, intubation tube 101, sleeve tube 102, top cover 103, main pipe 701, branch pipe 702.

detailed description

A reusable pneumatic ELISA sample loading device, the device is composed of a ventilating insertion row 7, an air pump 8, a plurality of sample loading tubes, a push rod 3 and an outer frame 10;

As shown in Figure 1, the sample loading tube includes an external sleeve 1 and a piston 2 in the sleeve; the upper end of the external sleeve 1 is sealed by a top cover 103, the upper part is cylindrical, the lower part is conical, and the piston 2 in the sleeve is sealed. It is located on the upper part of the external casing 1 and is in interference fit with the external casing 1, and the upper part of the piston 2 in the casing is a pressure chamber. The lower end of the push rod 3 is connected with the inner piston 2 of the sleeve, and the upper end passes through the top cover 103 and extends to the outside of the outer sleeve 1 . The push rod 3 and the piston 2 in the casing have a vertical through hole communicating with each other, and the upper end of the vertical through hole is sealed by a sealing cover 11 . The setting of the push rod 3 can, on the one hand, prevent the occurrence of individual liquids that are not fully injected into the holes. After the gas injection is completed, the push rod 3 that has not exhausted the liquid can also be manually pressed to ensure that all the liquids to be used are injected into the holes. On the other hand, the liquid required for the next step can be added through the vertical through hole to realize re-adding.

As shown in FIG. 2 , two cannulae 101 and two sleeves 102 are arranged around the pressure chamber, and both the cannula 101 and the sleeves 102 communicate with the pressure chamber. The cannula 101 and the cannula 102 are relatively distributed in position.

As shown in Figure 5, the ventilation insert 7 includes a main pipe 701 and a plurality of branch pipes 702 arranged in parallel on one side of the main pipe 701. The branch pipes 702 communicate with the main pipe 701 and are perpendicular to the main pipe 701; Cannula 101 or cannula 102 are connected.

As shown in Figure 3, the cannula 101 is inserted into the sleeve 102 of the adjacent sample loading tube, and a plurality of sample loading tubes are connected to each other through the cannula 101 and the sleeve 102 to form a sample loading matrix, and the unconnected cannula on the upper side 101 is connected with the ventilation insertion row 7, and then communicated with the air pump 8; the intubation tube 101 on the left side is sealed, and the sleeve tube 102 on the right side and the lower side are sealed.

A one-way valve plate 5 is installed on the lower part of the external casing 1, and the accommodating space for storing the liquid 6 to be loaded is below the one-way valve plate 5. The lower end of the external casing 1 is sealed by the socket base 4 .

The outer frame 10 is provided with a plurality of hollow holes, and the hollow holes are arranged in a matrix, corresponding to a 96-well plate; the front part of the sample loading tube is embedded in the hollow holes.

The working process of the device is as follows (take the 96-well plate as an example):

Place the outer frame 10 above the 96-well plate, insert the outer casing 1 into the hollow hole and fix it, remove the socket base 4 of each tube, align it with the sample hole, and then use the air pump 8 to inject air, and the piston 2 in the casing will move Press down to open the valve plate 5 in the tube, the gas pushes the liquid, and the liquid to be sampled is naturally injected into the sample loading hole 9, as shown in Figure 4. In order to prevent the situation that individual liquids are not fully injected into the holes, after the gas injection is completed, the push rod 3 of the unfilled liquid can also be manually pressed to ensure that all the liquids to be used are injected into the holes.

In addition, after the same type of liquid is loaded, the soft base 4 is put back, the piston 2 in the sleeve is pulled back to the initial position through the push rod 3, and then the sealing cover 11 on the top of the piston is opened, and the next step is added into the tube cavity. After adding all the required liquids, add samples together according to the method described above.

Claims (2)

1. a kind of repeatable vapour-pressure type ELISA sampling devices utilized, it is characterised in that the device is by ventilation insert row (7), air pump (8), multiple loading pipes, push rod (3) and outrigger (10) composition；The loading pipe includes external sleeve pipe (1) and piston-in-sleeve (2)；External sleeve pipe (1) upper end is sealed by top cover (103), and top is cylindrical shape, and bottom is taper, piston-in-sleeve (2) position In external sleeve pipe (1) top, it is interference fitted with external sleeve pipe (1), piston-in-sleeve (2) top is pressure chamber；Pressure chamber surrounding Two intubations (101) and two sleeve pipes (102) are disposed with, intubation (101) and sleeve pipe (102) are connected with pressure chamber, are intubated And sleeve pipe (102) Relative distribution in position (101)；Intubation (101) is inserted into the sleeve pipe (102) of adjacent loading pipe, multiple Loading pipe is connected with each other by being intubated (101) and sleeve pipe (102), constitutes loading matrix, the not connected intubation (101) of avris or set Pipe (102) is connected or sealed with ventilation insert row (7), and ventilation insert row (7) is connected with air pump (8)；

It is to treat loading liquid (6) for storing that external sleeve pipe (1) bottom, which is provided with below check valve sheet (5), check valve sheet (5), Accommodation space, external sleeve pipe (1) lower end is sealed by pullover base (4)；

Outrigger (10) is provided with multiple hollow holes, and hollow hole is arranged in matrix, and the pore size distribution with 96 orifice plates is corresponding；The front portion of loading pipe It is embedded in hollow hole；

Push rod (3) lower end is connected with piston-in-sleeve (2), and upper end passes through top cover (103), extends to external sleeve pipe (1) outside； The push rod (3) is provided with the vertical through hole connected with piston-in-sleeve (2), and vertical through hole upper end is sealed by closure (11).

2. sampling device according to claim 1, it is characterised in that the ventilation insert row (7) includes being responsible for (701) and many The individual branch pipe (702) for being arranged in parallel in supervisor (701) side, branch pipe (702) is communicated with supervisor (701), and is hung down with supervisor (701) Directly；The intubation (101) or sleeve pipe (102) that branch pipe (702) is not connected with avris are connected.