CN216051763U - A device for horseradish peroxidase-dependent colorimetric detection of lead ions - Google Patents
A device for horseradish peroxidase-dependent colorimetric detection of lead ions Download PDFInfo
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- CN216051763U CN216051763U CN202122025651.4U CN202122025651U CN216051763U CN 216051763 U CN216051763 U CN 216051763U CN 202122025651 U CN202122025651 U CN 202122025651U CN 216051763 U CN216051763 U CN 216051763U
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- Prior art keywords
- reaction
- reaction tube
- liquid storage
- storage bottle
- lead ion
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- 238000001514 detection method Methods 0.000 title claims abstract description 19
- 108010001336 Horseradish Peroxidase Proteins 0.000 title claims abstract description 12
- 230000001419 dependent effect Effects 0.000 title claims abstract description 10
- 150000002500 ions Chemical class 0.000 title 1
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 238000006243 chemical reaction Methods 0.000 claims abstract description 49
- 238000007789 sealing Methods 0.000 claims abstract description 23
- 108020004414 DNA Proteins 0.000 claims abstract description 20
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 15
- 102000053602 DNA Human genes 0.000 claims abstract description 13
- 238000011534 incubation Methods 0.000 claims abstract description 13
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000006911 enzymatic reaction Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 17
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 102000016911 Deoxyribonucleases Human genes 0.000 claims 1
- 108010053770 Deoxyribonucleases Proteins 0.000 claims 1
- 230000012447 hatching Effects 0.000 claims 1
- 238000003780 insertion Methods 0.000 abstract description 6
- 230000037431 insertion Effects 0.000 abstract description 6
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 239000002122 magnetic nanoparticle Substances 0.000 description 8
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 6
- 229960005156 digoxin Drugs 0.000 description 6
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 239000011550 stock solution Substances 0.000 description 6
- 101710163270 Nuclease Proteins 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 238000009413 insulation Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a device for horse radish peroxidase-dependent lead ion colorimetric sensing detection, which relates to the field of kits and comprises an insert block, wherein an incubation component is arranged in the insert block, a plurality of insertion holes are formed in the insert block, reaction tubes are inserted into the insertion holes, the middle lower parts of the reaction tubes are inserted into the incubation component, the upper parts of the reaction tubes are connected with magnetic rings in a sliding mode, sealing plugs are arranged at the tops of the reaction tubes, two sliding strips are symmetrically arranged at the tops of the sealing plugs, a deoxyribonucleic acid enzyme reaction liquid storage component is connected onto the sliding strips in a sliding mode, and the bottoms of the deoxyribonucleic acid enzyme reaction liquid storage component are communicated with the reaction tubes after the deoxyribonucleic acid enzyme reaction liquid storage component fully descends. In the utility model, each reaction tube and the accessory components thereof are an independent reaction detection system, and the detection can be finished only by sequential operation without additional split charging, thereby avoiding the error leakage or pollution caused by multi-step liquid addition.
Description
Technical Field
The utility model relates to the field of kits, in particular to a device for horse radish peroxidase-dependent lead ion colorimetric sensing detection.
Background
The existing lead ion detection kit is internally provided with independent multi-bottle detection reagents, which comprise a digoxin antibody labeled magnetic nanoparticle solution, GR-5 deoxyribonucleic acid nuclease solution, streptavidin modified HRP enzyme solution, TMB color development solution, stop solution and the like.
Need add multiple reagent in order in different steps in whole testing process, when the testing sample is more, the process that the reagent was added to the partial shipment is comparatively loaded down with trivial details, and the manual operation in-process adds or leaks with the reagent by mistake very easily, causes the testing failure, and the lid of in-process centrifuging tube that the partial shipment adds and reagent bottle is open state moreover, pollutes other materials very easily, causes the testing result deviation.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a device for horse radish peroxidase-dependent lead ion colorimetric sensing detection, which solves the defects that the reagent addition of the existing kit is easy to miss and pollute.
The utility model provides a device for horse radish peroxidase relies on type lead ion color comparison sensing detection, includes the inserted block, be equipped with in the inserted block and incubate the subassembly, be equipped with a plurality of jacks on the inserted block, it has the reaction tube to peg graft in the jack, the well lower part of reaction tube inserts in the subassembly of incubating, reaction tube upper portion sliding connection has the magnetism circle, the top of reaction tube is equipped with the sealing plug, the top symmetry of sealing plug is equipped with two slides, sliding connection has the DNA enzyme reaction liquid storage subassembly on the slide, the bottom of DNA enzyme reaction liquid storage subassembly is in abundant back and reaction tube intercommunication down.
Preferably, the DNA enzymatic reaction liquid storage assembly comprises a liquid storage bottle, a sliding groove is formed in the outer wall of the liquid storage bottle, the liquid storage bottle is connected with a sliding strip in a sliding mode through the sliding groove, a liquid inlet needle is communicated with the bottom of the liquid storage bottle, the lower portion of the liquid inlet needle is embedded into a sealing plug, and a top plug is connected to the top of the liquid storage bottle in a sealing mode.
Preferably, two supporting pieces are connected between the bottom of the liquid storage bottle and the top of the sealing plug in a virtual mode, and the supporting pieces are arranged between the two sliding strips.
Preferably, the incubation assembly comprises a water bath cavity arranged in the insert, the middle lower part of the reaction tube is inserted into the water bath cavity, and one side of the water bath cavity is communicated with a water injection tube.
Preferably, the insert block is externally wrapped with a heat insulation layer, and the water injection pipe penetrates out of the heat insulation layer.
The utility model has the advantages that:
in the utility model, each reaction tube and the accessory components thereof are an independent reaction detection system, and the detection can be finished only by sequential operation without additional split charging, thereby avoiding the error leakage or pollution caused by multi-step liquid addition. According to the utility model, the liquid storage bottle is connected above the sealing plug in a sliding manner, GR-5 deoxyribonucleic acid enzyme liquid can be conveniently injected into the reaction tube through pressing the liquid storage bottle downwards and the liquid inlet needle, and the GR-5 deoxyribonucleic acid enzyme liquid can be added into the digoxin antibody-labeled magnetic nanoparticle solution to form reaction liquid without uncovering, sucking and transferring, so that the pollution risk is reduced. The injection of the HRP enzyme liquid and the sample to be detected is finished by penetrating the syringe into the top plug, the sample to be detected is conveniently sucked by utilizing the residual negative pressure in the reaction tube, and the liquid is not required to be added after the cover is opened. The GR-5 DNA nuclease liquid can be effectively prevented from flowing out of the liquid storage bottle by inserting the lower end of the liquid inlet needle into the sealing plug. The incubation component can directly complete the incubation step in the insert, does not need to prepare a water bath incubation device, and is very portable. The support sheet can prevent the liquid inlet needle from being inserted into the reaction tube by mistake, and avoid causing wrong premixing in a non-working state. The magnetic ring can effectively adsorb and precipitate magnetic nano particles, and is convenient for separating supernatant for subsequent color reaction.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a top view of the present invention.
Fig. 3 is a sectional view taken along line a-a in fig. 2.
Fig. 4 is a sectional view taken along line B-B in fig. 2.
The device comprises an insertion block 1, a heat insulation layer 11, a water injection pipe 12, a water bath cavity 13, an insertion hole 14, a reaction pipe 2, a sealing plug 21, a magnetic ring 22, a sliding strip 3, a liquid storage bottle 4, a sliding chute 41, a top plug 42, a liquid inlet needle 5 and a support sheet 6.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the utility model easy to understand, the utility model is further described with the specific embodiments.
As shown in fig. 1 to 4, a device for horseradish peroxidase-dependent lead ion colorimetric sensing detection includes a plug block 1, an incubation component is arranged in the plug block 1, a plurality of insertion holes 14 are arranged on the plug block 1, a reaction tube 2 is inserted in the insertion holes 14, the middle lower part of the reaction tube 2 is inserted in the incubation component, the upper part of the reaction tube 2 is slidably connected with a magnetic ring 22, a sealing plug 21 is arranged at the top of the reaction tube 2, two sliding strips 3 are symmetrically arranged at the top of the sealing plug 21, a deoxyribonucleic acid enzyme reaction liquid storage component is slidably connected on the sliding strips 3, and the bottom of the deoxyribonucleic acid enzyme reaction liquid storage component is communicated with the reaction tube 2 after fully descending. The initial state in the reaction tube 2 is vacuum or negative pressure similar to vacuum, which is convenient for sucking the liquid in the upper DNA nuclease reaction liquid storage component and the subsequent sample liquid. The liquid storage bottle 4 is internally preloaded with GR-5 DNA nuclease liquid with single detection dosage, and the reaction tube 2 is preloaded with digoxin antibody-labeled magnetic nanoparticle solution with single detection dosage. The SAV-HRP enzyme liquid can be stored in the liquid storage bottle 4, and only the inner part of the liquid storage bottle 4 needs to be separated into two independent spaces, and the bottoms of the two independent spaces are communicated with the top of the liquid inlet needle 5. When the liquid inlet needle 5 is penetrated into the reaction tube 2, the SAV-HRP enzyme liquid and the GR-5 DNA nuclease liquid can be sucked into the reaction tube simultaneously and mixed with the digoxin antibody marked magnetic nano particle solution to form reaction liquid.
In this embodiment, the subassembly is stored to deoxyribonucleic acid enzyme reaction liquid includes stock solution bottle 4, be equipped with spout 41 on the outer wall of stock solution bottle 4, stock solution bottle 4 passes through spout 41 and 3 sliding connection of draw runner, the bottom intercommunication of stock solution bottle 4 has into liquid needle 5, it buries in sealing plug 21 to enter liquid needle 5 lower part, the top sealing connection of stock solution bottle 4 has top stopper 42. The sealing plug 21 and the top plug 42 are both made of elastic rubber. Stock solution bottle 4 is the cavity tube-shape, and its inner wall bottom is for leaking hopper-shaped and with advance the intercommunication of 5 tops of liquid needle, is convenient for like this during liquid gets into reaction tube 2 of below completely, reduces and remains.
In this embodiment, two support sheets 6 are virtually connected between the bottom of the liquid storage bottle 4 and the top of the sealing plug 21, and the support sheets 6 are arranged between the two sliding strips 3.
In this embodiment, the incubation assembly includes a water bath cavity 13 disposed in the insert block 1, the middle-lower portion of the reaction tube 2 is inserted into the water bath cavity 13, and one side of the water bath cavity 13 is communicated with a water injection tube 12. The insert block 1 is externally coated with a heat-insulating layer 11, and the water injection pipe 12 penetrates out of the heat-insulating layer 11. The heat dissipation of in-process thermal in the heat preservation 11 can effectual reduction incubation keeps the temperature, prevents that the temperature from crossing low the effect is hatched in the influence water bath.
The working process and principle are as follows:
before detection, the supporting sheet 6 which is virtually connected at two sides is torn off, the liquid storage bottle 4 is directly pressed down until the bottom of the liquid storage bottle 4 is contacted with the top of the sealing plug 21, at the moment, the lower end of the liquid inlet needle 5 is inserted into the reaction tube 2 and is communicated with the reaction tube 2 and the liquid storage bottle 4, and because the reaction tube 2 is internally provided with negative pressure, GR-5 deoxyribonucleic acid enzyme liquid in the liquid storage bottle 4 under normal pressure is completely sucked into the reaction tube 2 and is mixed and reacted with the digoxin antibody labeled magnetic nano particle solution. Then a certain amount of SAV-HRP enzyme liquid is injected into the liquid storage bottle 4 after being penetrated into the top plug 42 through an injector, the SAV-HRP enzyme liquid, the digoxin antibody marked magnetic nanoparticle solution and the GR-5 deoxyribonucleic acid enzyme liquid are mixed to form reaction liquid, then the sample solution to be detected is injected into the liquid storage bottle 4 after being penetrated into the top plug 42 through the injector, because a certain negative pressure still exists in the reaction tube 2, the sample to be detected is also sucked into the reaction tube 2 through the liquid inlet needle 5, the mixture is kept stand after being fully mixed, warm water is injected into the water bath cavity 13 through the water injection tube 12 until the water is full, the liquid in the reaction tube 2 is immersed in the warm water for incubation, the reaction tube 2 is pulled out after the incubation is finished, the magnetic ring 22 is pulled up and down until the magnetic nanoparticles are completely precipitated and adsorbed at the bottom of the reaction tube 2, all accessories on the sealing plug 21 and the sealing plug are removed, and the supernatant in the reaction tube 2 is sucked, carrying out subsequent TMB color development reaction.
It will be appreciated by those skilled in the art that the utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The embodiments disclosed above are therefore to be considered in all respects as illustrative and not restrictive. All changes which come within the scope of or equivalence to the utility model are intended to be embraced therein.
Claims (5)
1. The utility model provides a device that is used for horse radish peroxidase to rely on type lead ion colorimetric sensing to detect, its characterized in that, includes inserted block (1), be equipped with in inserted block (1) and hatch the subassembly, be equipped with a plurality of jacks (14) on inserted block (1), it has reaction tube (2) to peg graft in jack (14), the well lower part of reaction tube (2) is inserted in hatching the subassembly, reaction tube (2) upper portion sliding connection has magnetic ring (22), the top of reaction tube (2) is equipped with sealing plug (21), the top symmetry of sealing plug (21) is equipped with two draw runner (3), sliding connection has deoxyribonucleic acid enzyme reaction liquid storage assembly on draw runner (3), deoxyribonucleic acid enzyme reaction liquid storage assembly's bottom is in abundant back and reaction tube (2) intercommunication down.
2. The device for horseradish peroxidase-dependent colorimetric lead ion sensing detection according to claim 1, wherein the deoxyribonuclease reaction solution storage component comprises a solution storage bottle (4), a chute (41) is arranged on the outer wall of the solution storage bottle (4), the solution storage bottle (4) is slidably connected with the slide bar (3) through the chute (41), a solution inlet needle (5) is communicated with the bottom of the solution storage bottle (4), the lower part of the solution inlet needle (5) is embedded in the sealing plug (21), and a top plug (42) is hermetically connected to the top of the solution storage bottle (4).
3. The device for horseradish peroxidase-dependent colorimetric lead ion sensing according to claim 2, wherein two support sheets (6) are virtually connected between the bottom of the liquid storage bottle (4) and the top of the sealing plug (21), and the support sheets (6) are arranged between the two slide bars (3).
4. The device for horseradish peroxidase-dependent colorimetric lead ion sensing detection according to claim 1, wherein the incubation assembly comprises a water bath cavity (13) arranged in the insert block (1), the middle lower part of the reaction tube (2) is inserted into the water bath cavity (13), and one side of the water bath cavity (13) is communicated with a water injection tube (12).
5. The device for horseradish peroxidase-dependent lead ion colorimetric sensing detection according to claim 4, wherein the insert block (1) is externally coated with an insulating layer (11), and the water injection pipe (12) penetrates out of the insulating layer (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122025651.4U CN216051763U (en) | 2021-08-26 | 2021-08-26 | A device for horseradish peroxidase-dependent colorimetric detection of lead ions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122025651.4U CN216051763U (en) | 2021-08-26 | 2021-08-26 | A device for horseradish peroxidase-dependent colorimetric detection of lead ions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216051763U true CN216051763U (en) | 2022-03-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122025651.4U Expired - Fee Related CN216051763U (en) | 2021-08-26 | 2021-08-26 | A device for horseradish peroxidase-dependent colorimetric detection of lead ions |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216051763U (en) |
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2021
- 2021-08-26 CN CN202122025651.4U patent/CN216051763U/en not_active Expired - Fee Related
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220315 |
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| CF01 | Termination of patent right due to non-payment of annual fee |