CN206219570U - For the aseptic air-breather of algae culture - Google Patents

Abstract

The utility model relates to an aseptic ventilation device for algae cultivation. The device includes an air pump, a flow meter and a filter connected in series on the gas pipeline, and the main gas pipeline passing through the filter is connected with the gas transmission rack. Multiple air outlet nozzles are installed on each longitudinal air guide pipe on the air delivery rack, and an air outlet rubber hose is inserted at the lower port of each air outlet nozzle. The lower port is inserted into the inflatable through-tube inserted in the rubber stopper at the opening of the triangular flask. The inflatable through-tube passes through the rubber stopper that seals the upper opening of the triangular flask and then extends into the triangular flask. The lower port of the inflatable through-tube is plugged with The inflatable rubber hose in the bottle extends to the bottom of the triangular bottle, and at the same time, the exhaust pipe connecting the inside and outside of the triangular bottle is inserted on the rubber stopper. The utility model realizes the sterility of marine microalgae cultivation, and at the same time, the air flow rate is controllable, and a set of equipment can simultaneously provide multiple groups of cultivation, and the structure of the device also lays a foundation for large-scale industrial cultivation in the future.

Description

Aseptic aerators for algae cultivation

technical field

The utility model belongs to the technical field of algae cultivation equipment, in particular to an aseptic ventilation device for algae cultivation.

Background technique

Marine microalgae are very rich in nutrients. In addition to rich in protein, amino acids, pigments, etc., they are also rich in high-value nutrients and chemical raw materials such as EPA and DHA. At present, marine microalgae are widely used in the pharmaceutical industry, food industry and animal feed.

The cultivation of marine microalgae in the laboratory is carried out in triangular flasks, but this cultivation mode has certain limitations, and the gas can only enter through the sealing film, which cannot meet the CO ₂ demand required by microalgae. The air pump injects gas, which cannot achieve gas sterility, and then causes microalgae to infect bacteria; therefore, it is of great significance to develop an effective aseptic ventilation device for algae cultivation.

Contents of the invention

The purpose of the utility model is to overcome the deficiencies of the prior art and provide an aseptic ventilation device for algae cultivation.

The utility model solves its technical problem and realizes by taking the following technical solutions:

An aseptic ventilation device for algae cultivation, the device includes an air pump, the air outlet of the air pump is connected with an air pump outlet conduit, and a flow meter for controlling the flow of gas is connected in series on the air pump outlet conduit, and at the end of the flow meter outlet pipe Insert the inlet hose with the filter, the port of the inlet hose is plugged with the inlet nozzle of the filter, a filter group is installed inside the filter, the outlet nozzle of the filter is connected with the outlet hose of the filter, and the outlet hose The outer port of the gas transmission main pipe is plugged with a gas transmission main pipe, and the gas outlet end of the gas transmission main pipe is bent downward to communicate with the gas transmission rack. There are multiple longitudinal air ducts on a plane, and multiple downward air outlet nozzles are installed at regular intervals on each longitudinal air duct, and an air outlet hose is inserted into the lower port of each air outlet nozzle, and an air outlet hose is installed on the air outlet hose. The pipe clamp that controls the on-off of the air outlet hose, the lower port of the air outlet hose is plugged into the inflatable tube inserted in the rubber plug at the opening of the triangular bottle, and the inflatable tube passes through the rubber plug that seals the upper opening of the triangular bottle and then extends to the triangular bottle Among them, an inflatable rubber tube extending to the bottom of the triangular bottle is plugged into the lower port of the inflation tube, and at the same time, an exhaust pipe connected to the inside and outside of the triangular bottle is inserted on the rubber stopper.

Moreover, the filter group further includes an activated carbon layer at the gas inlet end and a bacteria filter membrane arranged side by side with the activated carbon layer, the activated carbon layer is used to adsorb moisture and large-sized particles in the gas, and the bacteria filter membrane adopts The filter membrane with a pore size between 0.20μm and 0.22μm is used to filter out bacteria in the gas.

Moreover, the air outlet pressure of the air pump is maintained between 1.05-1.08 atmospheres.

Moreover, the rear end of the exhaust pipe passes through the rubber plug and bends downward.

Moreover, the pipe clamp further comprises a rectangular parallelepiped clamping frame, a threaded perforation is opened on the upper top wall of the clamping frame, a threaded push rod is screwed in the perforation, and a screw cap is formed on the upper end of the push rod, A disc-shaped pressing plate is fixedly connected to the lower end of the ejector rod, and the rotating screw cap is controlled by the pressing plate to control the flow rate of the air outlet hose installed in the clamping frame.

Advantage and positive effect of the present utility model are:

1. The present invention solves the aseptic operation of ventilation in the microalgae cultivation process;

_2. The present invention can control the CO in the microalgae cultivation process by adjusting the flowmeter Total aeration volume;

3. The present invention can control the _CO2 ventilation of each Erlenmeyer flask by adjusting the pipe clamp 19.

4. The present invention is easy to operate and easy to use.

Description of drawings

Fig. 1 is the overall structural representation of the utility model;

Fig. 2 is the enlarged structural representation of filter in the utility model;

Fig. 3 is a schematic diagram of the enlarged structure of the pipe clamp in the utility model.

detailed description

The embodiments of the present invention will be described in further detail below in conjunction with the accompanying drawings. It should be emphasized that the following embodiments are illustrative rather than restrictive, and should not be used as limitations of the present invention.

A kind of aseptic ventilation device for algae cultivation, as shown in Figure 1, the device includes an air pump 12, the air outlet of the air pump is connected with an air pump outlet conduit 11, and a flowmeter 10 for controlling the gas flow is connected in series on the air pump outlet conduit , the end of the outlet pipe of the flowmeter is plugged with the inlet rubber hose 9 of the filter, the port of the inlet rubber hose is plugged with the inlet nozzle 8 of the filter, and a filter group 7 for filtering bacteria is installed inside the filter, and the filter The outlet nozzle 6 of the filter is plugged with the outlet rubber hose 5 of the filter, and the gas delivery main pipe 4 is plugged in the outer port of the outlet rubber hose. The bent frame includes a horizontal air duct 3, on which a plurality of longitudinal air ducts 2 on a plane are communicated at even intervals, and a plurality of downward air outlets are installed on each longitudinal air duct at equal intervals. Nozzle 1, an air outlet rubber hose 16 is plugged into the lower port of each air outlet nozzle, and a pipe clamp 19 for controlling the on-off of the air outlet rubber hose is installed on the air outlet rubber hose. The lower port of the air outlet rubber hose is inserted into the rubber plug 14 at the opening of the triangle bottle 15 The inflatable through-pipe 17 is plugged in, the inflatable through-pipe passes through the rubber plug on the upper opening of the sealed conical flask and then extends into the conical flask, and the lower port of the inflatable through-pipe is plugged with an inflatable rubber hose 18 extending to the bottom of the conical flask , Simultaneously, on the rubber stopper, insert and connect the exhaust pipe 13 inside and outside the triangular flask.

In the specific implementation of the present utility model, as shown in Figure 2, the filter screen group further includes an activated carbon layer 20 at the air inlet end and a bacteria filter membrane 21 arranged side by side with the carbon layer, and the activated carbon layer is used for adsorption Moisture and large-sized particles in the gas, the bacteria filter membrane adopts a filter membrane with a pore size between 0.20 μm and 0.22 μm to filter out the bacteria in the gas.

In the specific implementation of the utility model, in order to ensure uniform and stable inflation of the marine microalgae in the triangular flask, the air outlet pressure of the air pump is maintained between 1.05-1.08 atmospheric pressure, and this pressure is conducive to the stability of the filter Work, is conducive to the practical life of the filter group.

In the specific implementation of the utility model, the rear end of the exhaust pipe passes through the rubber plug and bends downward, so as to prevent the dust in the atmosphere from falling into the exhaust pipe and contaminating the marine microalgae in the triangular flask.

In the specific implementation of the present utility model, the pipe clamp further includes a cuboid clamp frame 24, on the upper top wall of the clamp frame, there is a threaded perforation, and a threaded ejector rod 23 is screwed in the perforation. The upper end of the push rod is shaped on a screw cap 22, and the lower end of the push rod is affixed with a disc-shaped pressing plate 25, and the rotating screw cap is controlled by the pressing plate to pass through the flow of the air outlet hose in the clamp frame.

In the specific implementation of the utility model, the air pump pumps the carbon dioxide gas into the pipeline of the device under reduced pressure or under pressure.

Claims (5)

1. An aseptic ventilation device for algae cultivation, characterized in that: the device comprises an air pump, the air outlet of the air pump is connected with an air pump outlet conduit, and the air pump outlet conduit is connected in series with a flowmeter for controlling the flow of gas. The end of the meter outlet pipe is plugged with the inlet hose of the filter, and the port of the inlet hose is plugged with the inlet nozzle of the filter. A filter group is installed inside the filter, and the outlet nozzle of the filter is connected with the outlet hose of the filter. Inserting, a gas transmission main pipe is inserted at the outer port of the outlet rubber hose, and the gas transmission end of the gas transmission main pipe is bent downward to communicate with the gas transmission rack. The gas transmission rack includes a horizontal air guide tube, and the horizontal air guide There are multiple longitudinal air ducts on a plane connected at regular intervals on the upper side, and multiple downward air outlets are installed on each longitudinal air duct at regular intervals, and an air outlet rubber tube is inserted into the lower port of each air outlet , Install the pipe clamp to control the on-off of the air outlet hose on the outlet hose, the lower port of the outlet hose is plugged into the inflatable tube inserted in the rubber plug at the opening of the triangular bottle, and the inflatable tube passes through the rubber on the upper opening of the sealed triangular bottle After the stopper, it extends into the triangular bottle, and the lower port of the inflatable tube is plugged with an inflatable hose extending to the bottom of the triangular bottle. 2. The aseptic ventilation device for algae cultivation according to claim 1, characterized in that: the filter group further comprises an activated carbon layer at the air inlet end and a cell filter membrane arranged side by side with the activated carbon layer, the The activated carbon layer is used to adsorb moisture and large-sized particles in the gas, and the bacteria filter membrane adopts a filter membrane with a pore size between 0.20 μm and 0.22 μm to filter out bacteria in the gas. 3. The aseptic aeration device for algae cultivation according to claim 1, characterized in that: the pressure of the air outlet of the air pump is kept between 1.05-1.08 atmospheres. 4. The aseptic ventilation device for algae cultivation according to claim 1, characterized in that: the end of the exhaust pipe passes through the rubber plug and bends downward. 5. The aseptic ventilation device for algae cultivation according to claim 1, characterized in that: said pipe clamp further comprises a cuboid clamp frame, and a threaded perforation is provided on the upper top wall of the clamp frame. A threaded ejector rod is screwed into the hole, a screw cap is made on the upper end of the ejector rod, and a disc-shaped pressure plate is fixedly connected to the lower end of the ejector rod. flow.