CN104248476B - A kind of simple and easy Novel experimental anesthetic machine - Google Patents

Abstract

The invention discloses a simple and new experimental anesthesia machine, which includes an oxygen pump and a centrifuge tube provided with a liquid anesthetic. The oxygen pump communicates with a first gas flow meter and a second gas flow meter respectively. The meter communicates with the centrifuge tube through the centrifuge tube inlet conduit, the second gas flow meter communicates with the tee, the tee communicates with the centrifuge tube through the centrifuge tube outlet conduit, and the tee also communicates with the anesthesia induction box or the breathing anesthesia box through the anesthesia catheter. The invention has many advantages such as high structural strength, stable structure, safety and reliability, easy concentration control, high quality and low price.

Description

A simple and new experimental anesthesia machine

technical field

The invention relates to the field of animal experiment devices, and more specifically relates to a simple and novel experimental anesthesia machine, which is suitable for anesthesia of small animals.

Background technique

The working principle of the respiratory anesthesia machine for animal experiments is to introduce the airflow into the anesthesia bottle through a mechanical circuit, and then promote the volatilization of the anesthetic agent, and the airflow carrying the anesthetic gas is introduced into the nasal cavity of the animal, and finally reaches all parts of the body through the animal's respiratory system and circulatory system. Thus, the central nervous system is directly inhibited, resulting in the effect of general anesthesia. The respiratory anesthesia machine is a semi-open device, which can be divided into several parts according to its working principle: air supply system, anesthetic agent volatilization system, anesthesia induction and maintenance system, residual gas removal system, etc.

The anesthesia machines sold on the market are mostly used in the medical field. Since they are applied to the human body, their designs are generally more precise, their structures are complex, and the corresponding costs are relatively high. Even the anesthesia machine used in animal research is expensive and difficult to popularize and practical. However, with the development of biology, animals are more and more widely used in biological experiments. Therefore, an anesthesia machine that is simple, fast, safe and can effectively anesthetize animals has become an urgent problem to be solved. The high cost of existing medical anesthesia machines and commercial animal anesthesia machines brings unnecessary waste. Some laboratories have designed their own anesthesia machines, but the effect is poor, and the leakage of residual gas is serious, which is not good for the health of experimenters. In view of this, the applicant designed this simple and practical anesthesia machine for laboratory animals. It has a simple and efficient design, a simplified structure, and is easy to operate. It can effectively anesthetize small experimental animals such as rats and mice. The anesthesia machine is low in cost, high in reliability, adjustable in concentration, convenient to carry, and can be widely used in biological laboratories.

In addition, considering the health of the experimenters, a residual gas elimination system is specially added in the design, which can prevent the residual gas from entering the laboratory and effectively protect the experimenters.

Contents of the invention

The purpose of the present invention is to solve the above-mentioned problems in the prior art, and provide a simple and new experimental anesthesia machine, which reduces the difficulty of operation, reduces the cost of animal anesthesia, and has a residual gas elimination system.

Above-mentioned purpose of the present invention is achieved through the following technical solutions:

A simple new experimental anesthesia machine, including an oxygenation pump, and also includes a centrifuge tube provided with a liquid anesthetic, the oxygenation pump communicates with the first gas flowmeter and the second gas flowmeter respectively, and the first gas flowmeter passes through the centrifuge tube The inlet conduit communicates with the centrifuge tube, the second gas flowmeter communicates with the tee, the tee communicates with the centrifuge tube through the centrifuge tube outlet conduit, and the tee also communicates with the anesthesia induction box through the anesthesia catheter.

The above-mentioned anesthesia induction box is a transparent sealed plastic lunch box.

A simple and new experimental anesthesia machine also includes a breathing anesthesia box connected with an anesthesia catheter, and a breathing port is opened on the breathing anesthesia box.

A simple and new experimental anesthesia machine also includes a vacuum pump which communicates with a breathing anesthesia box.

The inlet conduit of the centrifuge tube described above extends below the liquid level of the liquid anesthetic, and the outlet conduit of the centrifuge tube extends above the liquid level of the liquid anesthetic.

Compared with the prior art, the present invention has the following advantages:

1. The small anesthesia machine is simple in design, easy to operate and easy to carry. During the development process, the heavy high-pressure nitrogen cylinders and high-pressure oxygen cylinders were discarded, and only a small oxygen pump can be used to realize the air supply. Any experimenter can operate it correctly after simple learning. The device has high reliability and can be popularized and used.

2. The concentration of anesthetic agent is easy to adjust, and the concentration of anesthetic gas is produced by mixing anesthetic gas and air according to a certain ratio. Therefore, the gas flow monitoring of the small anesthesia machine is coordinated and controlled by two gas flow meters (the first gas flow meter and the second gas flow meter), thereby effectively adjusting the anesthetic concentration in the gas, thereby effectively controlling the anesthesia depth of small animals. The anesthesia machine controls the gas flow by an air flow meter, which is simple and efficient and saves drugs. The anesthesia machine can not only quickly induce anesthesia (<1min) for animals, but also maintain low anesthetic concentration to keep animals under anesthesia for a long time (>2h).

3. The vacuum pump can be used to guide the exhaust gas to the outside to prevent it from escaping into the laboratory, thereby achieving the purpose of protecting the experimenters.

4. The cost of the selected equipment is low, and the total price is much lower than that of commercial equipment on the market, so it should be extended to biological experiments. In addition, all systems are inside a cuboid acrylic board box, which is easy to carry and use; the adjustment knobs are installed on the outer wall, which is convenient for adjustment.

The present invention has the above-mentioned characteristics, has achieved the purposes of low cost, simple structure, easy operation and high efficiency, and is ideal equipment for animal experiments.

Description of drawings

Fig. 1 is a structural schematic diagram of the first embodiment of the present invention.

Fig. 2 is a schematic structural diagram of a second embodiment of the present invention.

In the figure: 1-anesthesia machine shell; 2-oxygenation pump; 3-first gas flow meter; 4-second gas flow meter; 5-anesthetic agent; 6-centrifuge tube (50ml centrifuge tube can be selected); 7-centrifuge Tube inlet; 8-centrifuge tube outlet; 9-tee; 10-anesthesia induction box; 11-breathing anesthesia box; 12-vacuum pump; 13-plate.

detailed description

The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings.

Example 1

As shown in Figure 1~2,

1. Air supply:

The gas flow supply of the present invention all comes from the aeration pump 2 . The aeration pump 2 has two outlets, one of which is introduced into the first gas flow meter 3 (250mL/min) through a conduit, and the flow rate can be adjusted to generate an air flow with a certain flow rate, which is introduced into the centrifuge tube through the inlet conduit of the centrifuge tube 6, the anesthetic gas can be generated; the other outlet is connected to the second gas flow meter 4 (1L/min) through the catheter, and the flow rate can be adjusted to generate an air flow with a certain flow rate, which is the same as the final gas flow through the centrifuge tube outlet 8 The anesthetic gas is mixed so that the anesthetic gas concentration can be diluted. Gas flow meters with two flow rates are used here, wherein the first gas flow meter 3 can easily fine-tune the gas flow rate, so as to facilitate the effective control of the concentration of the anesthetic gas; In addition to providing enough oxygen to animals, it can quickly reduce the concentration of anesthetic gas. The anesthesia machine shell 1 can be selected from a cuboid acrylic board box, and the oxygen pump 2, the first gas flow meter 3, the second gas flow meter 4, the centrifuge tube 6, and the centrifuge tube air inlet 7 are all arranged in the anesthesia machine shell 1. The adjustment knobs of the first gas flow meter 3 and the second gas flow meter 4 are arranged on the anesthesia machine shell 1 , and the mouth of the centrifuge tube 6 protrudes from the anesthesia machine shell 1 .

2. Anesthesia induction:

The anesthesia induction box 10 can be an ordinary transparent sealed plastic lunch box, which not only can observe the anesthetized state of the animal conveniently, but also is very easy to obtain. The anesthesia induction box 10 is not only much cheaper than the commercial induction box, but also it is easy to find alternatives of various volumes and sizes, so that it is suitable for animals of different sizes. During use, a small opening on the side and top is sufficient.

Pour the liquid anesthetic (e.g. isoflurane, halothane) from the top into a 50ml centrifuge tube and screw the cap on to seal the gas. In order to facilitate the addition of liquid anesthetic at any time without affecting the formation and release of anesthetic gas, the centrifuge tube 6 must undergo a simple modification, that is, add two small holes on the side of the centrifuge tube 6 that are the same size as the outer diameter of the catheter, one of which is a centrifuge tube The air inlet and the other is the air outlet of the centrifuge tube. Wherein, the air inlet of the centrifuge tube is directly connected to the air outlet of the first gas flow meter 3 through the air inlet conduit of the centrifuge tube. In order to facilitate the generation of high-concentration anesthetic gas, the outlet of the centrifuge tube inlet conduit in the centrifuge tube 6 must extend to the bottom of the centrifuge tube 6 or directly contact the bottom of the centrifuge tube; Above the liquid level of the liquid anesthetic. At the same time, the connection between the centrifuge tube inlet conduit, the centrifuge tube outlet conduit and the centrifuge tube needs to be sealed with glass glue. The generated anesthetic gas will be mixed with the air exported by the second gas flow meter 4 through the tee 9, and the mixed gas will be directly introduced into the anesthesia induction box through the anesthesia catheter.

Turn on the oxygen pump 2, adjust the flow rate of the first gas flow meter 3 and the second gas flow meter 4, thereby controlling the concentration of the anesthetic agent. During the animal induction anesthesia process, it is necessary to use a high concentration of anesthetic gas, that is, increase the flow rate of the first gas flow meter 3 to generate a higher concentration of initial anesthetic gas, and at the same time reduce the flow rate of the second gas flow meter 4 as much as possible, In turn, a small amount of air that dilutes the anesthetic gas is produced, thereby producing a high concentration of anesthetic gas. If the concentration of the anesthetic gas needs to be reduced, the reverse operation is required, that is, the flow rate of the first gas flow meter 3 is decreased, and the flow rate of the second gas flow meter 4 is increased.

After the anesthetic agent volatilizes, it is output through the air outlet 8 of the centrifuge tube, and is transported to the T-shaped junction point of the tee through the catheter, mixed with the air input through the second gas flow meter 4, and then reaches the anesthesia induction box to induce anesthesia for small animals (test The anesthesia time for both rats and mice was less than 1 min).

3. Maintenance of anesthesia

After the animal is anesthetized, transfer it to the flat panel 13, connect the breathing anesthesia box with the anesthesia catheter, place the mouth and nose of the animal at the breathing anesthesia port of the breathing anesthesia box, and maintain the anesthesia state;

Adjust the ratio of the first gas flow meter 3 and the second gas flow meter 4 to reduce the concentration of the anesthetic agent, so as to maintain the animal anesthesia state efficiently for a long time (the duration of anesthesia for test rats and mice can be greater than 2 hours);

In order to protect the researchers, the residual gas is sucked out by the vacuum pump 12 and discharged out of the laboratory through the pipeline.

During use, if the total amount of anesthetic is too low, then turn off the oxygen pump 2, unscrew the centrifuge tube 6, add the anesthetic, screw on the cover, and turn on the power supply for controlling the oxygen pump 2 and the vacuum pump 12 again. It is convenient and easy to operate without affecting the whole system. Turn off the anesthesia machine by turning off the power.

The specific embodiments described herein are merely illustrative of the spirit of the invention. Those skilled in the art to which the present invention belongs can make various modifications or supplements to the described specific embodiments or adopt similar methods to replace them, but they will not deviate from the spirit of the present invention or go beyond the definition of the appended claims range.

Claims (1)

1. a simple and easy Novel experimental anesthetic machine, including oxygen increasing pump (2), it is characterized in that, also include the centrifuge tube (6) being provided with liquid anaesthetic agent, oxygen increasing pump (2) connects with the first gas flowmeter (3) and the second gas flowmeter (4) respectively, first gas flowmeter (3) is connected with centrifuge tube (6) by centrifuge tube air induction conduit, second gas flowmeter (4) connects with threeway (9), threeway (9) is connected with centrifuge tube (6) by centrifuge tube outtake tube, threeway connects with induction of anesthesia box (10) also by anesthetic tube；

Induction of anesthesia box (10) is transparent sealing plastic lunch box；

Also include breathing anesthesia box (11) connected with anesthetic tube, breathe and anesthesia box (11) offers pneumostome；

Also including vacuum pump (12), vacuum pump (12) connects with breathing anesthesia box (11)；

Described centrifuge tube air induction conduit extends to below the liquid level of liquid anaesthetic agent, and described centrifuge tube outtake tube extends to the ullage of liquid anaesthetic agent。