CN1916616A - Reusable bioassay test strips - Google Patents

Abstract

A biological test piece which can be used repeatedly, the main structure is formed by pasting a plurality of layers of thin slices, and the space formed by punching holes on the thin slices can be used for placing biological reaction carriers, and the test piece can be easily broken off, so as to be beneficial to secondary and tertiary reuse; two positive and negative electrodes are arranged between the sheets to conduct electrochemical signals of reaction, and the electrode wires are designed to have the same resistance in each electrochemical reaction area, so that the test piece can be repeatedly used. The test piece and the chemical carrier contained in the test piece can be used for matching with a biological sensing detection device, and detecting the components of the detected product and measuring the concentration of the detected product according to the electrochemical effect of the detected product in biological reaction; furthermore, two or more test items, which may be the same or different, may be measured simultaneously for a drop of blood. Meanwhile, the invention discovers for the first time that the addition of the carbon nanotube in the chemical carrier can greatly increase the biological reaction rate and shorten the detection time.

Description

Reusable bioassay test strips

technical field

The present invention relates to a biological detection test piece for personal use, in particular to a blood biological detection test piece that can be used multiple times in a single piece, and a biological detection test that can simultaneously measure two or more identical or different test items with one drop of blood. piece.

Background technique

At present, all blood glucose test strips on the market are test strips that can only be used once and are thrown away after use. Such test strips are too expensive and unaffordable for diabetic patients in developing or undeveloped countries, and cannot regularly test blood sugar according to their condition, and even endanger their lives. They desperately need a cheaper blood glucose test strip. At the same time, the blood glucose test strips currently on the market are not accurate enough, limited by the materials and methods they use, often resulting in an error of more than 30%. Moreover, except for a few brands in Europe and the United States, it takes fifteen to thirty seconds to measure, which is relatively long.

Therefore, there is a need to provide a blood glucose test strip that is cheap, more accurate, and has a shorter measurement time. Alternatively, it is necessary to provide a structural design and method that can be used multiple times on a single disposable, disposable biological detection test piece, so as to reduce the economic burden of the user. The inventor of this case first proposed an electrochemical test strip that can be used multiple times on a single test strip in U.S. Patent Application No. 10/704,701, but the content is different from this application. The test piece has the problem that it is not easy to break, and the resistance generated between the multiple reaction areas on the same test piece and the electrode wire tip is not the same, resulting in a considerable error in the measured detection value, and there is an improvement necessary.

In addition, U.S. Patent No. 5,120,420 applied by Shiro Nankai et al. discloses a scheme in which multiple electrode wires are placed on one test piece, which can detect the blood glucose concentrations of multiple test items at the same time, and then take the average value to increase the accuracy. However, this method requires a relatively large amount of blood, which is not feasible for patients to self-test at home. Simultaneously, this method also does not help to reduce the detection cost of the user.

Furthermore, there are many research reports and patents proposing various methods to improve measurement accuracy or detection speed. For example, US Patent No. 6,491,803 of Thomas Y.S.Shen mentions the use of nanometer metal powder to increase conductivity and reduce reaction time, but it still requires fifteen seconds of measurement time. Hart (Biosensors & Bioelectronics, 13:333-339, 1996) found that adding platinum powder to the surface of the electrode can increase the conductivity to improve the accuracy of the measurement, but these effects are not good enough. US Patent No. 6,541,216 to C.D. Wisley et al. states that plasma treatment of the surfaces of silver and carbon electrodes can increase the accuracy of test strip measurements. However, the above-mentioned methods cannot achieve the required goals of being economically cheaper, more accurate in measurement, and faster in measurement speed.

Contents of the invention

Therefore, the main purpose of the present invention is to provide a blood glucose test strip that is cheaper, more accurate and takes less time to measure.

Another main purpose of the present invention is to provide a blood glucose detection test piece, as long as one drop of blood is taken and one pain is taken, a variety of identical or different reading values can be measured with one drop of blood; multiple readings can be measured with one drop of blood The purpose of reading the same test product is to do more blood glucose tests and obtain the average value to increase the accuracy, but it is not necessary to collect blood many times, which can reduce the pain of blood collection; and several different blood glucose can be measured with one drop of blood The purpose of the reading value of the inspection product can also be used to test different items at the same time.

In order to achieve the above-mentioned purpose, the present invention provides a biological detection test piece that can be used repeatedly, which mainly includes a structure of a disposable, disposable electrode test piece that can be used multiple times and the structure of the electrode test piece The chemical carrier contained in it is used in conjunction with the biosensing detection device, and according to the electrical effect generated by the test product in the biological reaction, the composition of the test product is detected and its concentration is determined. A specific structure of the present invention is formed by pasting four layers of thin sheets, and the space formed by various punching holes on the thin sheet can produce three identical reaction zones, and a biological reaction carrier is placed on each reaction zone, only A blood volume of 2 μl is required. By the principle of siphon, the blood will be sucked into the reaction area and then electrochemically react with the biological reaction carrier on it, and the blood sugar of the blood sample can be obtained by analyzing the results of the electrochemical reaction concentration. At the same time, the thin sheet also has a space formed by various punching holes, so that the used test piece can be easily broken due to stress concentration factors, so as to facilitate the second or third use of other unused parts; There are two positive and negative conductive electrodes on the thin sheet to conduct the electrochemical signal of the reaction, and the electrode wire is designed so that the positive and negative electrodes of each electrochemical reaction zone have the same resistance as the positive and negative electrodes on the measuring electrode head , having the same resistance is the key factor for the multiple use of the test piece disclosed in the present invention, and it is also an indispensable element, and this method is adopted in the present invention for the first time. The positive and negative electrodes in each electrochemical reaction area will transmit the electrochemical reaction signal generated by the chemical carrier on it and the blood reaction to the positive and negative electrodes on the measuring electrode head, and then the blood glucose testing machine can interpret the blood sample. Blood glucose reading. As for the formula of the chemical reaction substance developed by the present invention, it contains hydroxypropyl methylcellulose (hydroxypropyl methylcellulose), so that its chemical reaction substance is evenly distributed, which is the same as the carboxymethylcellulose (Carboxymethylcellulose) adopted in U.S. Patent 5,120,420, It is different from the microcrystalline cellulose (Microcrystalline cellulose) used in Taiwan TW374116 and US Patent No. 6,413,394B1, which is used in the present invention for the first time.

In addition, the present invention also finds that by adding multi-walled and single-walled (Multi-walled and single walled) carbon nanotubes to increase the conduction rate and reduce the detection time, it can change the reaction time from the general thirty seconds Reduced to four seconds or even two seconds, and can provide appropriate surface tension and surface energy to prevent blood coagulation. In this way, the test strip provided by the present invention can be used repeatedly, and very accurate results can be obtained very quickly.

Furthermore, if the test piece formed by the above four layers is pasted on the top layer with the same four or eight layers, but the blood siphoning positions are all at the same position, then one drop of blood can be siphoned to two or more Three different and independent reaction areas are used for the same or different biological reaction detection, so more than two same or different items can be measured at the same time with one drop of blood. If one drop of blood is used to measure two or more of the same test items at the same time, the average value can be used to increase its accuracy; but if one drop of blood is used to measure two or more different items at the same time, the pain of each blood collection can be reduced .

According to a specific embodiment of the present invention, the present invention provides a biological detection test piece that can be used multiple times, mainly consisting of four layers of sheets, two wires with more than two groups of working electrodes and reference electrodes, and a test piece placed in the reaction area. Composed of chemical carriers; the four-layer sheet includes a bottom layer on which two electrode wires with more than two groups of working electrodes and reference electrodes are pasted, a second layer with three independent reaction zone punching holes on three small sheets, and three There are three independent blood siphon concave third layers on the small sheet, and a fourth layer with three independent gas outlets on the three small sheets; wherein the two wires with more than two groups of working electrodes and reference electrodes, the Each working or reference electrode has the same resistance to the electrode tip.

According to another specific embodiment of the present invention, the present invention also provides another biological detection test piece that can be used repeatedly, mainly consisting of three layers of sheets, two wires with more than two groups of working electrodes and reference electrodes, and a set Composed of chemical carriers in the reaction area; the three-layer thin sheet includes the bottom layer with two electrode wires with more than two groups of working electrodes and reference electrodes on it, and the second layer with three independent blood siphon concaves on three small sheets. layer, and a third layer with three independent gas outlets on three small slices; wherein the two wires with more than two groups of working electrodes and reference electrodes have the same resistance from each working electrode or reference electrode to the electrode head.

According to another specific embodiment of the present invention, the present invention also provides another bioassay test strip that can be used multiple times, and one drop of blood can test three identical or different detection items, mainly composed of eight layers, twelve layer or more than sixteen layers, and four, six or more than eight wires with each group of wires having more than two sets of working electrodes and reference electrodes, and chemical carriers placed in the reaction zone; the eight layers, twelve Sheets with more than 16 layers include the bottom layer with two electrode wires with more than two sets of working electrodes and reference electrodes, the second layer with three independent reaction zone punching holes on three small sheets, and the upper layer with three small sheets. The third layer with three independent blood siphons and the fourth layer with three independent air outlets on three small slices; the two wires with more than two sets of working electrodes and reference electrodes, each working electrode Or the same resistance from the reference electrode to the electrode tip.

According to another specific embodiment of the present invention, the present invention also provides another bioassay test strip that can be used multiple times, and one drop of blood can test three identical or different detection items, mainly composed of six layers and nine layers. or more than twelve layers of sheets, and four, six or more than eight wires, each group of wires has more than two sets of working electrodes and reference electrodes, and chemical carriers placed in the reaction area; six, nine or twelve layers The sheets above the first layer include the bottom layer with two electrode wires with more than two sets of working electrodes and reference electrodes, the second layer with three small sheets with three independent blood siphon concaves, and the three small sheets with three independent blood siphons. The third layer of the gas outlet; wherein the two wires with more than two groups of working electrodes and reference electrodes have the same resistance from each working electrode or reference electrode to the electrode head.

According to another specific embodiment of the present invention, the present invention also provides another bioassay test piece that can be used multiple times and has a blood inlet on the side. The lead wire of the electrode and the chemical carrier placed in the reaction area; the three-layer thin sheet includes the bottom layer with two electrode wires with more than two groups of working electrodes and reference electrodes on it, and the bottom layer with three independent blood siphons. The second layer, and the third layer with three independent gas outlets; wherein the two wires with more than two sets of working electrodes and reference electrodes have the same resistance from each working electrode or reference electrode to the electrode head.

According to another specific embodiment of the present invention, the present invention also provides another biological detection test strip that can be used multiple times, the blood inlet is on the side, and one drop of blood can test three identical or different detection items, mainly composed of six layer, nine or more than twelve layers of sheets, four, six or more than eight and each group of wires has more than two groups of working electrodes and reference electrodes, and chemical carriers placed in the reaction area; the six layers, Sheets with nine or more than twelve layers include the bottom layer with two electrode wires with more than two sets of working electrodes and reference electrodes, the second layer with three independent blood siphons, and three independent gas outlets The third layer; wherein the two wires with more than two groups of working electrodes and reference electrodes have the same resistance from each working electrode or reference electrode to the electrode head.

According to a specific embodiment of the present invention, in the reusable biological detection test piece provided by the present invention, the base material of the sheet can be polyvinyl chloride (PVC) board, glass fiber (FR-4) board, polyether Sulfone (polyether sulphone, PES) board, bakelite board, PET board, PC board, glass board or ceramic board (CEM-1). The base material of the electrode wire is silver glue or carbon glue wire used in screen printing technology. The electrode wire can produce the required conductive circuit by the chemical reaction method of the additive process; it can also remove the partially useless conductor on the surface by the chemical reaction method of the subtractive process; and then use the chemical reaction method The immersion plating method (commonly known as gold plating) replaces precious metals (such as gold, platinum and other precious metals) on the substrate through chemical displacement reactions.

According to a specific embodiment of the present invention, in the reusable biological detection test strip provided by the present invention, the chemical carrier component in each reaction zone of the test strip comprises: hydroxypropyl methylcellulose (Hydroxypropyl methylcellulose) , Citric acid, Dibasic potassium phosphate, Non-ionic surfactant Triton X-100, Glucose oxidase, Potassium ferricyanide, Deionized water water), carbon nanotubes (Nanotube carbon).

Preferably, the content of the hydroxypropylmethylcellulose is 0.05%-2%. The carbon nanotubes can be multi-walled and single-walled (Multi-walled and single walled) carbon nanotubes, the size of which is 10 μm-200 μm, and the content is 0.01%-2%. The rest of the components in the chemical carrier formulation of the present invention are commonly used carriers for general blood sugar or other specimen tests, and their content ranges are conventional.

According to another specific embodiment of the present invention, the present invention also provides another bioassay test strip that can be used repeatedly, comprising several detection zones with chemical carriers on the test strip, and each two detection zones There is a breaking gap between them, so that it can be used for optical detection of the inspection product, and each piece can be used multiple times for blood glucose detection test strips.

In summary, the biological detection test piece provided by the present invention can be reused many times, and its main structure is formed by pasting multiple layers of sheets, and the space formed by punching holes on the sheet can place biological reaction carriers. , and the test piece can be easily broken to facilitate the second or third reuse; the electrode wires used to conduct the electrochemical signal of the reaction between the sheets are designed to have the same resistance in each electrochemical reaction area, and the measurement is accurate high degree. The test piece of the present invention and the chemical carrier contained therein can be used in conjunction with a biosensing detection device, and detect the composition of the test product and determine its concentration according to the electrochemical effect produced by the test product in the biological reaction; and , It can also measure more than two identical or different inspection items at the same time with one drop of blood. At the same time, the invention has the advantages of high accuracy and short testing time.

Description of drawings

FIG. 1 is a three-dimensional assembly schematic diagram of the multiple-usable biological detection test piece of the present invention.

FIG. 2 and FIG. 2A are respectively a three-dimensional exploded schematic diagram of the multiple-usable biological detection test piece of the present invention, and a schematic diagram of another embodiment of the wires arranged on the base sheet.

3A-3D are schematic cross-sectional views of the bioassay test piece that can be used multiple times in the present invention.

Fig. 4 is a three-dimensional combination schematic diagram of a bioassay test piece of the present invention that can be used multiple times and can test three identical or different detection items with one drop of blood.

Fig. 5A and Fig. 5B are schematic cross-sectional views of a bioassay test strip of the present invention that can be used multiple times and can test three same or different test items with one drop of blood.

FIG. 6 is a three-dimensional assembly diagram of a biological detection test piece that can be used multiple times and has a blood inlet on the side of the present invention.

Fig. 7 is a schematic diagram of an embodiment of a conductive electrode in a biological detection test strip that can be used multiple times and has a blood inlet on the side of the present invention.

Fig. 8 is a three-dimensional combination schematic diagram of a biological detection test piece that can be used multiple times, the blood inlet is on the side, and one drop of blood can test three identical or different detection items.

Fig. 9 is a schematic diagram of another embodiment of the present invention.

Fig. 10 is a graph of electrochemical reactions between chemical carriers with and without carbon nanotubes and blood.

Explanation of main component symbols in the figure:

Test piece 10, 90; Thin slice 11, 12, 13, 14;

Small flakes 121, 122, 123, 131, 132, 133, 141, 142, 143;

Punching holes 201, 202, 203, 401, 402, 403, 404, 405, 406, 17, 18;

Concave 19, 191, 192, 193, 194, 195, 196, 301, 302, 303;

chemical carrier 251, 252, 253, 91, 92, 93; blood 40;

Blood inlets 71, 72, 73, 74, 75, 76, 77, 78, 79; electrode heads 51, 52;

Lead wires 15, 16; working electrodes 161, 162, 163; reference electrodes 151, 152, 153.

Detailed ways

In order to enable readers to have a deeper understanding and understanding of the structure and features of the present invention, the feasible embodiments are listed below in detail with accompanying drawings:

Please refer to Fig. 1, Fig. 2, Fig. 2A and Fig. 3A-Fig. and two wires 15,16 are placed between the sheets 11,12, the wire 15 is connected to three reference electrodes 151,152,153, and the wire 16 is connected to three working electrodes 161,162,163 (as shown in Figure 2 shown). The working electrodes 161, 162, 163 can be designed as a circle, while the reference electrodes 151, 152, 153 are arranged at the gaps of the working electrodes 161, 162, 163 and are not in contact with each other; or The working electrodes 161, 162, 163 are designed as an H shape (as shown in FIG. 2A ) or square, and the reference electrodes 151, 152, 153 are arranged at the gaps of the working electrodes 161, 162, 163 and are not in contact with each other. type.

There are two punch holes 17, 18 on the sheet 11, which can generate stress concentration to facilitate the test piece to break. There is also an indentation 19 on the sheet 11 to mark the position of blood entry.

Thin slice 12 is divided into three small slices 121,122,123 and sticks on the wire 15,16 and thin slice 11; There is a gap in the middle of these three small slices 121,122,123 for the test piece after the combination is relatively easy to break (as shown in Figure 3C shown), and the three small slices 121, 122, 123 each have punching holes 201, 202, 203 to place chemical carriers 251, 252, 253 on the working electrodes 161, 162, 163 and reference electrodes 151, 152, 153 (as shown in FIG. 3A ) to form three different reaction zones, and an indentation 191, 192, 193 is respectively provided at the ends of the three small slices 121, 122, 123 to mark the position of blood entry.

Thin slice 13 is divided into three small flakes 131,132,133 and sticks on the thin slice 12, likewise, there is a gap in the middle of the three small flakes 131,132,133 for the combined test piece is relatively easy to break; and on it Having three indentations 301 , 302 , 303 allows the blood 40 to inhale and react electrochemically with the chemical carriers 251 , 252 , 253 through the siphon principle (as shown in FIGS. 3A and 3B ). Therefore, the test strip only needs 2 μl of blood for each test. By the principle of siphon, the blood will be sucked into the reaction area and then electrochemically react with the biological carrier on it, and by analyzing the result of the electrochemical reaction, it can Know the blood glucose concentration of the blood sample.

The sheet 14 is divided into three small sheets 141, 142, 143 and pasted on the sheet 13, and there are three indentations 194, 195, 196 on it to mark the position of blood inflow; there are also punched holes 401, 402, 403 on it as siphons Principle blood outlet.

And lead wire 15,16 is then designed as reference electrode 151,152,153 needs to have identical wire length to electrode head 51, namely same resistance, and working electrode 161,162,163 needs to have identical wire length to electrode head 52 , that is, the same resistance, so that the biological reaction results in the three different reaction zones will be consistent. As mentioned above, the same resistance of the lead wires 15 and 16 is a key factor and an indispensable element for the repeated use of the test piece disclosed by the present invention. This method is adopted in the present invention for the first time in the world.

In the above structure, the sheets 11, 12, 13, 14 can be plastic plates such as polyvinyl chloride (PVC), polyether sulfone (PES), PET, PC, etc., or ceramic substrates, bakelite boards, cardboard or FRP (glass fiber reinforced plastics) material; and wires 15, 16 can be silver glue or carbon glue wires used in the screen printing technology, and can also produce the required chemical reaction method with the addition method (Additive Process). Conductive circuit; Subtractive process can also be used to remove local useless conductors on the surface by chemical reaction; and then precious metals (such as gold, platinum, etc.) The chemical replacement reaction is replaced on the substrate. In this way, the thickness of the precious metal can be averaged when more than 100 pieces are produced at a time, that is, the resistance of the three reaction areas on each piece of the test piece can be maintained at 100%. error within one. The method of the present invention is different from U.S. Patent 5,437,999, and is also different from China Taiwan TW 496110, because the latter utilizes the electroplating method to plate precious metals, and all test pieces need to be connected to the same electrode (the method of the present invention does not need), which will cause test The thickness of the precious metal plating on the chip is not uniform, and the resistances in the three reaction zones all exceed an error of more than 30%, so the error is too large to be applicable.

When the present invention is in use with the above-mentioned structure, please refer to FIGS. 3A-3D again, which show the application process of the present invention when blood 40 enters the test strip 10 . When the blood 40 enters and fills the reaction zone in the punching hole 201 through the siphon effect from the concave 301 at the right end of the test strip 10 (as shown in FIGS. 3A to 3B ), after a few seconds and the blood glucose reading value appears, The test piece of the used first reaction zone can be broken off (as shown in FIG. 3C ), and the unused second reaction zone in the middle (as shown in FIG. 3D ) is exposed, and then the next blood test can be carried out . By analogy, after the second reaction zone is used up, the test piece of the second reaction zone can be broken off to expose the third unreacted reaction zone, and then the third reaction zone can be carried out on this test piece 10. The same test piece can be used multiple times for multiple blood tests.

Please refer to Fig. 4, Fig. 5A and Fig. 5B again, which shows an embodiment diagram in which three groups of test strips 10 shown in Fig. 1 with the same structure and different lengths are overlapped and combined into one piece, which allows blood to enter The entrance positions of the punching holes 301 are the same, and the punching holes 404 , 405 , 406 serving as air outlets penetrate at least from the top to the bottom sheet, so three identical or different items can be tested simultaneously with one drop of blood. When this embodiment is used, as shown in Fig. 5A-Fig. 5B, when a drop of blood 40 is sucked and filled with three reaction zones a, b and c overlapped at its right side, the chemical carrier 251 in each reaction zone will be simultaneously with The blood 40 sucked into each reaction area initiates an electrochemical reaction. Wherein the chemical carrier 251 in the three reaction zones a, b, c can be the same, to produce the same reading value, and then get the average value to obtain better accuracy; also can be different types, with a drop Three different readings were obtained for the blood. Similarly, this test piece can be broken and reused after the first test.

The test piece structure described above can also be changed and implemented as shown in Figure 6 and Figure 7. In the embodiment shown in Figure 6, the test piece 10 is designed to be formed by pasting three layers of sheets 11, 12, 13, wherein the inner Concave blood inlets 71, 72, 73 are arranged on the second layer of sheet 12, and are all designed on the other side of the electrode heads 51, 52 in the direction of extension, so that blood can enter through the side blood inlets 71, 72, 73, and Perforations 404, 405, 406 (through at least the bottommost sheet) are air outlets for the blood siphon. In addition, the design form of the wires 15, 16 can be changed in response to different blood inlet positions (as shown in Figure 7), and the chemical carrier 251 is coated on the working electrodes 161, 162, 163 of the first layer of sheet 11 and the reference On the reaction zone formed by the electrodes 151, 152, 153; according to this, the purpose that the same test piece 10 can be used for multiple detections in the embodiment shown in FIG. 1 can be achieved. Again, the test strip that this blood enters from the side can also be designed as a multi-stack implementation (as shown in Figure 8), wherein the blood inlets 71, 72, 73, 74, 75, 76, 77, 78, 79 It is located on the side of the test piece 10, and the punching holes 404, 405, 406 are the air outlets of the blood siphon, so it can also achieve multiple tests with one drop of blood as described in the embodiment shown in Figure 4 Efficacy of the test piece. As mentioned above, the test strips of each segment can be further stacked to more than four or five groups of reaction zones.

As mentioned above, the structure of a test piece that can be used multiple times, the present invention also proposes another embodiment of the design suitable for the few blood glucose testers (such as: ROCHE) on the market that use optical methods to detect blood glucose concentration, such as As shown in Figure 9, three reaction zones with chemical carriers 91, 92, 93 can be provided on a test piece 90, and two gaps 94 are correspondingly provided on two sides between every two reaction zones, so as to be able to Easily snap off the used reaction zone.

As for the chemical carrier formula in the reaction zone adopted in the present invention, its composition can be composed of hydroxypropyl methylcellulose (Hydroxypropyl methylcellulose), citric acid (Citric acid), dibasic potassium phosphate (Dibasic potassium phosphate), nonionic Composed of active surfactant TritonX-100, Glucose oxidase, Potassium ferricyanide, Deionized water, and Nanotube carbon.

Wherein hydroxypropylmethyl cellulose is adopted for the first time in the present invention, in order to make the chemical carrier evenly distribute when drying on the test piece, its content is preferably 0.05%-2%; Citric acid is used to adjust the pH value, while It also has an anticoagulant effect; dipotassium hydrogen phosphate is used to adjust the pH value; non-ionic surfactant Triton X-100 is used to reduce the surface tension of water and help dissolve additives; glucose oxidase It will produce an electrochemical reaction with blood; red blood salt is used as a conductive medium; carbon nanotubes are also used for the first time in the present invention to increase the reaction rate and reduce the reaction time, as shown in FIG. 10 . Carbon nanotubes can be used or not; they can reduce the reaction time and are not absolutely necessary elements. Among the above components, the content of the hydroxypropylmethylcellulose is preferably 0.05%-2%; the carbon nanotubes can be multi-walled and single-walled carbon nanotubes, the size of which is preferably 10 μm-200 μm, and the content is preferably 0.01%-2%; the rest of the components are commonly used carriers for general blood sugar or other specimen tests, and their content ranges are conventional.

A specific embodiment of the chemical carrier formula in the reaction zone of the present invention can be:

1. Hydroxypropyl methyl cellulose (Hydroxypropyl methyl cellulose), content: 0.4%

2. Citric acid (Citric acid), content: 0.085%

3. Dibasic potassium phosphate, content: 0.43%

4. Nonionic surfactant Triton X-100, content: 0.035%

5. Glucose oxidase, content: 0.7%

6. Red blood salt (Potassium ferricyanide), content: 3.5%

7. Deionized water (Deionized water), content: 94.8%

8. Nanotube carbon, content: 0.05%

The aforementioned chemical carriers 251-253 are placed on the working electrodes 161-163 and reference electrodes 151-153 in the punching holes 201-203 with a Musashi precision dispenser to absorb the biologically active substances of the carrier and conduct electricity. Medium, and make the electrode part from hydrophobic to hydrophilic, so as to facilitate the adhesion of the sample, and further protect the biologically active material from being damaged during the drying process at a higher temperature; while the aforementioned biologically active material is only Including immobilized or even unimmobilized enzymes (such as glucose oxidase Glucose oxidase), antigens, antibodies, microbial cells, animal and plant cells, and components with biorecognition capabilities of animal and plant tissues. Products (such as blood and other biological tissues) produce biological or chemical reactions; and the above-mentioned conductive medium (such as Potassium ferricyanide, quinones) content of 3.5%, is used to receive the electrons generated by the reaction between the enzyme and the test product, and then pass the electrons through The electrode wire is passed to the sensor to convert the concentration of the test product.

Claims (15)

1, a kind of reused biological detecting test piece, mainly by four layers of thin slice, two by the lead of working electrode and reference electrode more than two groups and place the chemistry carrier of reaction zone to be formed; Described four layers of thin slice comprise post two bottoms that the electrode cable of two groups of above working electrodes and reference electrode arranged, have have on three flakelets three independently the reaction zone punching the second layer, have and have three independently the 3rd layer of blood siphon indent and have three independently the 4th layers of gas outlet are arranged on three flakelet on three flakelets; Wherein said two leads that two groups of above working electrodes and reference electrode are arranged, its each working electrode or reference electrode are identical to the resistance of electrode tip.

2, a kind of reused biological detecting test piece, mainly by three layers of thin slice, two by the lead of working electrode and reference electrode more than two groups and place the chemistry carrier of reaction zone to be formed; Described three layers of thin slice comprise post two bottoms that the electrode cable of two groups of above working electrodes and reference electrode arranged, have have on three flakelets three independently the second layer and having of blood siphon indent three independently the 3rd layers of gas outlet are arranged on three flakelet; Wherein said two leads that two groups of above working electrodes and reference electrode are arranged, its each working electrode or reference electrode are identical to the resistance of electrode tip.

3, a kind of reused and one bleed and can check the biological detecting test piece of three kinds of identical or test items inequality, mainly by eight layers, Floor 12 or the thin slice more than 16 layers, and four, more than six or eight and every group of lead has the lead of working electrode and reference electrode more than two groups and place the chemistry carrier of reaction zone to be formed; Described eight layers, Floor 12 or the thin slice more than 16 layers comprise post two bottoms that the electrode cable of two groups of above working electrodes and reference electrode arranged, have have on three flakelets three independently the reaction zone punching the second layer, have and have three independently the 3rd layer of blood siphon indent and have three independently the 4th layers of gas outlet are arranged on three flakelet on three flakelets; Wherein said two leads that two groups of above working electrodes and reference electrode are arranged, its each working electrode or reference electrode are identical to the resistance of electrode tip.

4, a kind of reused and one bleed and can check the biological detecting test piece of three kinds of identical or test items inequality, mainly by six layers, nine layers or thin slice more than the Floor 12, and four, more than six or eight and every group of lead has the lead of working electrode and reference electrode more than two groups and place the chemistry carrier of reaction zone to be formed; The above thin slice of six layers, nine layers or Floor 12 comprise post two bottoms that the electrode cable of two groups of above working electrodes and reference electrode arranged, have have on three flakelets three independently the second layer and having of blood siphon indent three independently the 3rd layers of gas outlet are arranged on three flakelet; Wherein said two leads that two groups of above working electrodes and reference electrode are arranged, its each working electrode or reference electrode are identical to the resistance of electrode tip.

5, a kind of can repeatedly use and blood inlet at the biological detecting test piece of side, mainly by three layers of thin slice, two by the lead of working electrode and reference electrode more than two groups and place the chemistry carrier of reaction zone to be formed; Described three layers of thin slice comprise post two bottoms that the electrode cable of two groups of above working electrodes and reference electrode arranged, have three independently blood siphon indent the second layer and three independently the 3rd layers of gas outlet are arranged; Wherein said two leads that two groups of above working electrodes and reference electrode are arranged, its each working electrode or reference electrode are identical to the resistance of electrode tip.

6, a kind ofly can repeatedly use, the blood inlet is in the side and one bleed and can check the biological detecting test piece of three kinds of identical or test items inequality, mainly by six layers, nine layers or thin slice more than the Floor 12, four, more than six or eight and every group of lead have the lead of two groups of above working electrodes and reference electrode and place the chemistry carrier of reaction zone to form; The above thin slice of described six layers, nine layers or Floor 12 comprise post two bottoms that the electrode cable of two groups of above working electrodes and reference electrode arranged, have three independently blood siphon indent the second layer and three independently the 3rd layers of gas outlet are arranged; Wherein said two leads that two groups of above working electrodes and reference electrode are arranged, its each working electrode or reference electrode are identical to the resistance of electrode tip.

7, as each described test piece of claim 1-6, the base material of wherein said thin slice is polyvinyl chloride panel, glass mat, polyethersulfone plate, bakelite plate, PET plate, PC plate, glass plate or ceramic plate.

8, as each described test piece of claim 1-6, the base material of wherein said electrode cable is elargol or the carbon paste lead that is used for screen printing technology.

9, as each described test piece of claim 1-6, the chemistry carrier composition in each reaction zone of this test piece comprises: HPMC, citric acid, dipotassium hydrogen phosphate, nonionic surfactant Triton X-100, glucose oxidase ferment, potassium ferricyanide, deionized water, CNT.

10, test piece as claimed in claim 8, wherein said electrode cable are that the chemical reaction method with addition process produces required conducting channel; Or to subtract the chemical reaction method of multiplication, the conductor that surface local is useless is removed; And then with electroless plated method with noble metal by chemical replacement reaction displacement on ground.

11, test piece as claimed in claim 9, wherein said chemistry carrier contains many walls and Single Walled Carbon Nanotube, and its size is 10 μ m-200 μ m, and content is 0.01%-2%.

12, test piece as claimed in claim 9, wherein said chemistry carrier contains HPMC, and content is 0.05%-2%.

13, a kind of reused biological detecting test piece, be included in this test piece and be provided with most detection zones with chemistry carrier, and between per two detection zones, be provided with the breach that fractures, detect the inspection product can be used for optical means, and reach the every nonexpondable blood sugar test test piece of energy.

14, as each described test piece of claim 1-6, wherein said working electrode is designed to circle, and reference electrode then is arranged at the indentation, there of this working electrode and is non-touching kenel.

15, as each described test piece of claim 1-6, wherein said working electrode is designed to H shape, and reference electrode then is arranged at the indentation, there of this working electrode and is non-touching kenel.

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