CN209559811U - A multi-parameter electrochemical detection electrode sheet - Google Patents

Abstract

The utility model provide it is a kind of it is easy to operate, prepare liquid can be prevented contaminated, and compact-sized multi-parameter electrochemical detection electrode piece.The electrode slice, including substrate, reference electrode and multiple working electrodes, each working electrode have respective syphon runner, and electrode slice further includes pipette, and pipette is fixedly connected with substrate, and pipette is connected to multiple syphon runners.Prepare liquid is sucked into pipette, by syphon runner is guided to reference electrode and each working electrode again.Syphon runner has siphon port, and siphon port is located at the long side of substrate, and pipette is fixedly connected on the substrate long side, and pipette is connected to by siphon port with multiple syphon runners.

Description

A multi-parameter electrochemical detection electrode sheet

technical field

The utility model relates to the field of electrochemical detection, in particular to a multi-parameter electrochemical detection electrode sheet.

Background technique

The following background art is used to help readers understand the utility model, and should not be regarded as prior art.

Screen-printed electrodes based on the principle of electrochemical biosensors are widely used in the field of point-of-care detection (POCT). Physiological fluids such as blood, urine, sweat, and saliva contain many parameters of interest to clinical laboratories. For example, glucose, cholesterol, glycosylated hemoglobin, blood ketones in blood, uric acid in urine, lactic acid in sweat, etc. The detection principle of the electrode is to modify the enzyme corresponding to the target analyte on the working electrode, and realize the rapid quantitative analysis of the target analyte by detecting the enzymatic reaction current of the target analyte.

In order to realize simultaneous detection of multiple parameters, Chinese patent application CN103048462A discloses a multi-parameter electrochemical immunosensor based on an electrode array. The specific electrode uses multiple sets of detection electrodes, counter electrodes and reference electrodes to detect the contents of multiple tumor markers. During detection, the sensor electrodes are placed in the liquid to be tested for immune reaction. The disadvantages of this patent application are: 1. It is necessary to collect more liquid to be tested to infiltrate all the electrodes; 2. The liquid to be tested is conductive. Mutual interference affects the accuracy of detection; 3. The structure is not compact enough. When the detection parameters need to be increased, the size of the electrode sheet must be widened to accommodate more detection electrodes. In other existing technologies, when performing multi-parameter detection, after sampling, it is necessary to use a dropper or other pipetting device to drop the sample into the sample port, and the liquid to be tested is shunted from the sample port to each working electrode, generate electrical signals. The entire process of sampling, transferring, and adding samples is cumbersome, and the liquid to be tested is easily contaminated during the transfer process.

Utility model content

The purpose of the utility model is to provide a multi-parameter electrochemical detection electrode sheet which is convenient to operate, can prevent the liquid to be tested from being polluted, and has a compact structure.

A multi-parameter electrochemical detection electrode sheet, including a substrate, a reference electrode and a plurality of working electrodes, is characterized in that: each working electrode has its own siphon flow channel, and the electrode sheet also includes a pipette, a pipette It is fixedly connected with the substrate, and the suction pipe communicates with multiple siphon channels. The liquid to be tested is sucked into the pipette, and then guided to the reference electrode and each working electrode through the siphon flow channel.

Further, the siphon flow channel has a siphon port, the siphon port is located on the long side of the substrate, the suction pipe is fixedly connected to the long side of the substrate, and the liquid suction pipe communicates with a plurality of siphon flow channels through the siphon port. Preferably, the axis of the suction pipe is perpendicular to the axis of the siphon channel.

Further, the pipette is a capillary, and the inner surface of the pipette is coated with a hydrophilic film. Preferably, the pipette has a nozzle for sucking the liquid to be tested into the pipette, the nozzle is located at one end of the pipette, and the other end of the pipette is provided with absorbent cotton. Preferably, the suction nozzle is flush with one end of the substrate, or the suction nozzle protrudes from the substrate.

Further, a plurality of partitions are arranged in the siphon channel, and the partitions divide the siphon channel into a plurality of sub-channels. Preferably, the sub-channel is parallel to the axis of the siphon flow channel.

Further, a plurality of siphon channels are commonly connected to a reference electrode. That is, multiple working electrodes share one reference electrode. Preferably, the reference electrode runs through all the siphon channels, and the reference electrode is perpendicular to the axis of the siphon channels.

Further, the reference electrode is closer to the siphon port relative to the working electrode.

Further, the working electrode has a connection with the siphon flow channel, and the distance between the connection of multiple working electrodes and the corresponding siphon opening is equal.

Further, the siphon flow channel is provided with a pressure sensor for sending out electrical signals when the liquid to be tested flows through and a blinking signal light for receiving electrical signals. The pressure sensor is far away from the siphon port relative to the working electrode, and the signal light is electrically connected to the pressure sensor. When the liquid to be tested flows through the siphon channel, flows through the working electrode, and reaches the pressure sensor, the pressure sensor generates an electrical signal and transmits the electrical signal to the signal lamp, and the signal lamp flashes after receiving the electrical signal, indicating that the liquid to be tested has been connected to the working electrode. touch.

Further, each working electrode is modified with a biochemical reagent for detecting target analyte, multiple working electrodes are modified with the same biochemical reagent, or multiple working electrodes are modified with different biochemical reagents. The modification of different biochemical reagents on multiple working electrodes may be that each working electrode is modified with different biochemical reagents, and the number of working electrodes is greater than or equal to the number of types of biochemical reagents.

Further, the biochemical reagent is glucose oxidase, mutarotase, glucose dehydrogenase, lactate oxidase, urate oxidase, cholesterol oxidase, phospholipase, amino acid enzyme, horseradish peroxidase or beta-hydroxybutyrate dehydrogenase Hydrogenase.

The beneficial effects of the utility model:

1. The suction pipe directly absorbs the liquid to be tested, and guides the liquid to be tested to the working electrode through the siphon flow channel, saving the steps of sampling, transferring, and dropping samples, making the operation more convenient, and preventing the liquid to be tested from being damaged during the transfer process. Pollution; the pipette is a capillary tube, and a small amount of the liquid to be tested can form a capillary action in the pipette to be guided to the working electrode, so that the liquid to be tested can be fully utilized.

2. Each working electrode has its own siphon flow channel. When multiple parameters are detected at the same time, the electrical signals generated by each working electrode will not interfere with each other, which improves the accuracy of detection.

3. There is a pressure sensor and a signal light in the siphon flow channel, which are used to indicate whether the liquid to be tested flows through the working electrode, so as to avoid the detection error caused by the blockage of the siphon flow channel or the suction pipe.

4. Multiple parallel sub-channels are set in the siphon flow channel, which can speed up the flow of the liquid to be tested to the working electrode through the siphon flow channel.

5. Multiple siphon ports are located on the same long side of the substrate, and the long side of the substrate is larger in size, which can increase the number of working electrodes and siphon flow channels while maintaining the original size of the substrate, making the structure more compact and saving space , Saving materials.

6. Since the siphon flow channel is connected to the same reference electrode, that is, multiple working electrodes share one reference electrode, which saves materials; in addition, the reference electrode is closer to the siphon port than the working electrode, so that the liquid to be tested first passes through the reference electrode Then flow to each working electrode, avoid the enzymatic reaction on each working electrode from affecting the potential of the reference electrode, and improve the detection accuracy.

Description of drawings

Fig. 1 is a schematic diagram of the appearance of a multi-parameter electrochemical detection electrode sheet in an embodiment of the present invention.

Fig. 2 is a schematic diagram of an electrode layer in an embodiment of the present invention.

Fig. 3 is a schematic diagram of the diversion layer in an embodiment of the present invention.

Fig. 4 is a schematic diagram of a cover plate in an embodiment of the present invention.

Detailed ways

Below in conjunction with specific embodiment the utility model is further described in detail.

A multi-parameter electrochemical detection electrode sheet, as shown in Figure 1, includes a substrate, a reference electrode 104 and a plurality of working electrodes 101, working electrodes 102 and working electrodes 103, each working electrode has its own siphon flow channel 301, The electrode sheet also includes a liquid suction tube 2 , which is fixedly connected to the substrate, and the liquid suction tube 2 communicates with a plurality of siphon flow channels 301 . The liquid to be tested is sucked into the pipette, and then guided to the reference electrode and each working electrode through the siphon flow channel. Pipettes can be made of plastic, glass, metal or alloys. As shown in Figure 2 and Figure 3, the substrate comprises an electrode layer 1 and a conduction layer 3 stacked on each other, and the reference electrode 104 and the working electrode 101, the working electrode 102 and the working electrode 103 are all arranged on the electrode layer 1; the conduction layer 3 is provided with a notch, and the notch and the electrode layer 1 jointly form a siphon flow channel. As shown in FIG. 4 , the electrode sheet further includes a cover plate 4 , and the flow guide layer 3 is located between the cover plate 4 and the electrode layer 1 . The cover plate 4 is located on the upper surface of the flow guide layer 3 , and the electrode layer 1 is located on the lower surface of the flow guide layer 3 .

As shown in Figure 1, the siphon channel 301 has a siphon port 3011, the siphon port 3011 is located at the long side of the substrate, the liquid suction pipe 2 is fixedly connected to the long side of the substrate, and the liquid suction pipe 2 passes through the siphon port 3011 and a plurality of The siphon channel 301 is connected. The axis of the suction pipe 2 is perpendicular to the axis of the siphon channel 301 . The long sides of the substrate are larger in size and can accommodate more siphon flow channels, which can increase the number of working electrodes and siphon flow channels while maintaining the original size of the electrode sheet.

The pipette is a capillary tube, and the inner surface of the pipette is coated with a hydrophilic film. As shown in FIG. 1 , the pipette 2 has a nozzle 201 for sucking the liquid to be tested into the pipette. The nozzle 201 is located at one end of the pipette, and the other end of the pipette is provided with absorbent cotton 202 . Absorbent cotton prevents excess blood from spilling from the other end of the pipette. The ends of the pipette are flush with the ends of the long sides of the substrate. The suction nozzle is flush with one end of the substrate.

In some embodiments, the suction nozzle may protrude beyond the substrate.

Electrochemical detection electrodes are commonly used to detect the content of target analytes in blood or urine. Taking the detection of blood as an example, after piercing the skin with a puncturer and extruding a blood drop, the suction nozzle of the pipette is aimed at the blood drop, and the blood drop is guided into the pipette under capillary action. When the blood flows through each siphon port, it is guided to the reference electrode and the working electrode by the siphon channel. Squeeze out as much blood as you need for testing, avoid taking out excess blood and causing waste, and there is no need to transfer blood to prevent blood contamination and improve detection accuracy.

In some embodiments, a plurality of partitions are arranged in the siphon channel, and the partitions divide the siphon channel into a plurality of sub-channels. The sub-channel is parallel to the axis of the siphon flow channel. As shown in FIG. 3 , the partition 303 may be a part of the diversion layer 3 , and the partition is located in the notch of the diversion layer and is perpendicular to the axis of the notch. The partition makes the notch a comb-like shape. In some embodiments, one side of the separator is fixedly connected to the electrode layer, and the other side of the separator is flush with the upper end surface of the siphon flow channel. The upper end surface of the siphon flow channel refers to the side of the siphon flow channel facing the cover plate. When it is necessary to have a large contact area between the working electrode and the liquid to be tested to increase the electrical signal, it is necessary to increase the lateral dimension of the siphon channel, which will reduce the flow rate of the liquid to be tested in the siphon channel, and the setting of the sub-channel It can speed up the flow rate of the liquid to be tested in the siphon channel. In addition, the siphon flow channel is covered with a cover plate, and the partition plate can prevent the cover plate from sinking under the action of an external force and adhering to the bottom surface of the siphon flow channel, resulting in blockage of the siphon flow channel.

In this embodiment, all siphon channels share a cover plate, and a hydrophilic film is provided on the back of the cover plate. The back side of the cover plate refers to the side facing the siphon flow channel when the cover plate is covered on the base plate. In some embodiments, there may be multiple cover plates, and each siphon channel has its own cover plate.

As shown in FIG. 1 , a plurality of siphon channels 301 are commonly connected to a reference electrode 104 . That is, multiple working electrodes share one reference electrode. The reference electrode 104 runs through all the siphon channels 301 , and the reference electrode 104 is perpendicular to the axis of the siphon channels 301 . The reference electrode 104 is closer to the siphon opening 3011 relative to the working electrode 101 , the working electrode 102 and the working electrode 103 . In each siphon flow channel, the liquid to be tested is drained to the reference electrode and the working electrode in turn.

The working electrode and the siphon channel 301 have connection points, and the distances between the connection points of multiple working electrodes and the corresponding siphon ports 301 are equal. As shown in Figure 1 and Figure 2, the working electrode includes a first section, a second section and a third section connected in sequence, the first section and the third section are parallel, the second section is perpendicular to the first section and the third section, and the second section is perpendicular to the first section and the third section. The third section is connected to the siphon flow channel, and the third section is perpendicular to the axis of the siphon flow channel. Each working electrode has a corresponding working electrode terminal 105, and the working electrode terminal 105 is used to connect to an electrochemical workstation, and the electrochemical workstation is used to detect the electrical signal of the working electrode. The suction nozzle of the pipette faces away from the working electrode terminal, the working electrode terminal 105 is located at one end of the substrate, and the suction nozzle 201 of the pipette is flush with the other end of the substrate.

The siphon channel 301 is provided with a pressure sensor 3021 for sending out electrical signals when the liquid to be tested flows through and a flashing signal lamp 3022 for electrical signals. The pressure sensor 3021 is far away from the siphon port 3011 relative to the working electrode, and the signal lamp 3022 is connected to the pressure sensor. 3021 electrical connections. When the liquid to be tested flows through the siphon channel, flows through the working electrode, and reaches the pressure sensor, the pressure sensor generates an electrical signal and transmits the electrical signal to the signal lamp, and the signal lamp flashes after receiving the electrical signal, indicating that the liquid to be tested has been connected to the working electrode. touch.

Each working electrode is modified with a biochemical reagent for detecting a target analyte, multiple working electrodes are modified with the same biochemical reagent, or multiple working electrodes are modified with different biochemical reagents. The biochemical reagent is glucose oxidase, mutarotase, glucose dehydrogenase, lactate oxidase, urate oxidase, cholesterol oxidase, phospholipase, amino acid enzyme, horseradish peroxidase, or beta-hydroxybutyrate dehydrogenase .

When multiple working electrodes are modified with the same biochemical reagent, it can be used to verify the accuracy of the detection results. For example, the working electrode 101, the working electrode 102 and the working electrode 103 are all modified with glucose oxidase for detecting the glucose content in the blood, and the electrochemical workstation detects that the current of the working electrode 101 is I ₁ and the current of the working electrode 102 is I ₂ , The current of the working electrode 102 is I ₃ , and the absolute value of the calculated current difference ∆I ₁ =|I ₁ -I ₂ |, ∆I ₂ =|I ₁ -I ₃ |, ∆I ₃ =|I ₂ -I ₃ |. ∆I ₁ , ∆I ₂ and ∆I ₃ represent the correctness of the test results, and the smaller the value, the higher the accuracy of the test results. The concentration of glucose in the detection sample can be calculated correspondingly through the currents I ₁ , I ₂ and I ₃ .

The multiple working electrodes modified with different biochemical reagents may be that each working electrode is modified with different biochemical reagents, and the number of working electrodes is greater than or equal to the number of types of biochemical reagents. The number of working electrodes is equal to the number of types of biochemical reagents, each working electrode can be used to detect a substance, and as many substances as there are working electrodes can be detected. For example, the working electrode 101, the working electrode 102, and the working electrode 103 are respectively modified with glucose oxidase, urate oxidase, and lactate oxidase, and the electrochemical workstation detects that the current of the working electrode 101 is I ₄ , and the current of the working electrode 102 is I ₅ 1. The current of the working electrode 102 is I ₆ , and the concentration of glucose, uric acid and lactic acid in the detection sample can be calculated respectively through the values of I ₄ , I ₅ and I ₆ .

In some embodiments, the number of working electrodes is greater than the number of types of biochemical reagents. For example, there are 6 working electrodes in total. Glucose oxidase is modified on working electrodes A ₁ and A ₂ to detect the glucose content in the test solution, and urate oxidase is modified on B ₁ , B ₂ and B ₃ to detect The content of uric acid in the liquid to be tested, the lactate oxidase modified on _C1 , is used to detect the content of lactic acid in the liquid to be tested.

The content described in the embodiments of this specification is only an enumeration of the realization forms of the utility model concept, and the protection scope of the utility model should not be regarded as being limited to the specific forms stated in the embodiments, and the protection scope of the utility model also extends to Equivalent technical means that those skilled in the art can think of according to the concept of the utility model.

Claims (8)

1. A multi-parameter electrochemical detection electrode sheet, comprising a substrate, a reference electrode and a plurality of working electrodes, is characterized in that: each working electrode has its own siphon flow channel, and the electrode sheet also includes a pipette, a suction pipe The liquid pipe is fixedly connected with the base plate, and the suction pipe communicates with a plurality of siphon channels. 2. multi-parameter electrochemical detection electrode sheet as claimed in claim 1, is characterized in that: siphon channel has siphon mouth, and siphon mouth is positioned at the long side of substrate, and pipette is fixedly connected with described substrate long side, The suction pipe communicates with a plurality of siphon channels through the siphon port. 3. The electrode sheet for multi-parameter electrochemical detection according to claim 2, characterized in that: the pipette is a capillary, and the inner surface of the pipette is coated with a hydrophilic film. 4. multi-parameter electrochemical detection electrode sheet as claimed in claim 3, is characterized in that: pipette has the suction nozzle that is used to inhale liquid to be measured to the pipette, and suction nozzle is positioned at an end of pipette, and liquid suction The other end of the pipe is provided with absorbent cotton. 5. The multi-parameter electrochemical detection electrode sheet as claimed in claim 4, characterized in that: the siphon channel is provided with a plurality of partitions, and the partition divides the siphon channel into a plurality of sub-channels, and the sub-channels are separated from the siphon. The axes of the runners are parallel. 6. The electrode sheet for multi-parameter electrochemical detection according to claim 5, characterized in that: a plurality of siphon channels are connected to a reference electrode; the reference electrode is closer to the siphon opening relative to the working electrode. 7 . The electrode sheet for multi-parameter electrochemical detection according to claim 6 , wherein the working electrode has a connection with the siphon channel, and the distance between the connection of multiple working electrodes and the corresponding siphon opening is equal. 8. The electrode sheet for multi-parameter electrochemical detection as claimed in claim 7, characterized in that: the siphon channel is provided with a pressure sensor for sending an electrical signal when the liquid to be tested flows through and a flashing sensor for receiving an electrical signal. The signal light, the pressure sensor is far away from the siphon port relative to the working electrode, and the signal light is electrically connected to the pressure sensor.