CN1851459A - Multi-parameter whole blood integrating test bar - Google Patents
Multi-parameter whole blood integrating test bar Download PDFInfo
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- CN1851459A CN1851459A CN 200510011615 CN200510011615A CN1851459A CN 1851459 A CN1851459 A CN 1851459A CN 200510011615 CN200510011615 CN 200510011615 CN 200510011615 A CN200510011615 A CN 200510011615A CN 1851459 A CN1851459 A CN 1851459A
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Abstract
多参数全血集成试条，属于医学生化测试技术领域。为了弥补现有试条只能进行单一参数测试的局限性，本发明基于生物酶技术，提供了一种具有多参数测试功能的全血检测集成试条，它结合相应的检测仪器能够分时或分步地对血糖、尿酸或胆固醇等生理参数进行测量。本发明所述试条以高分子材料合成的基底为主体，基底之上依次覆盖导电银层、导电碳层、由绝缘漆形成的绝缘层、不干胶层和由亲水性高分子材料组成的覆盖膜层。本发明将葡萄糖氧化酶、尿酸氧化酶或胆固醇氧化酶分别设在同一基底上，由试条侧面同一进样口进样，但各反应池相互分开，电化学反应时互不干扰。
The multi-parameter whole blood integrated test strip belongs to the technical field of medical biochemical testing. In order to make up for the limitation that the existing test strips can only test a single parameter, the present invention provides a whole blood test integrated test strip with multi-parameter test function based on bio-enzyme technology. Step-by-step measurement of physiological parameters such as blood glucose, uric acid or cholesterol. The test strip of the present invention has a substrate synthesized by a polymer material as the main body, and the substrate is covered with a conductive silver layer, a conductive carbon layer, an insulating layer formed by insulating varnish, a self-adhesive layer, and a hydrophilic polymer material. the covering film layer. In the present invention, glucose oxidase, uric acid oxidase or cholesterol oxidase are respectively set on the same substrate, and samples are injected from the same inlet on the side of the test strip, but the reaction pools are separated from each other, and the electrochemical reaction does not interfere with each other.
Description
Technical field
The present invention relates to electric chemical formula bio-sensing strip, belong to the biochemical technical field of measurement and test of medical science.
Background technology
At present, the whole world has 100,012,000 diabetics, and developed countries onset diabetes rate has reached 2%～10%, domesticly should the disease population size have also surpassed 4,000 ten thousand people, accounts for about 4% of total population.Diabetes have become the 3rd human dead killer, and its incidence of disease is just with annual 10% speed increase, but recall rate has only 20%, that is to say have 80% patient to get diabetes but do not know, these people overwhelming majority are early stage patients, because of not knowing that the state of an illness very easily increases the weight of.The diabetic wishes the blood glucose value of oneself testing oneself every day at home very much, and in time adjusts dosage by the result that tests oneself.
Uric acid is the final metabolic product of purine.All can cause the rising (high lithemia disease) of blood plasma uric acid concentration or reduce (low uric acid disease) the acatharsia of uric acid if purine metabolic disturbance, energetic supersession reach kidney unusually.At present medical circle is thought, testing uric acid is the best biochemical marker of gout due to the diagnosis purine metabolic disturbance, thus in the blood uric acid be determined at significant clinically.
Disclose according to the Ministry of Public Health, the hypertensive patient of China surpasses 6,000 ten thousand, every studies show that, and increasing of cholesterol is the main arch-criminal of angiocardiopathies such as cardiac trigger disease, miocardial infarction.Hospital's tradition method for determination of cholesterol is based on colorimetric and method for saponification at present.This method is time-consuming and cost an arm and a leg, and needs specialized equipment and professional operation, is difficult for popularizing and the individual uses.The cholesterol test equipment be non-mainstream product at present, but because of heart disease and cardiovascular patient increase day by day, its market demand day by day enlarges, and especially is fit to the portable testing set of family's use.
In sum, blood sugar, uric acid and cholesterol are three important parameters by the blood testing health, and the present used strip of various instruments both domestic and external mostly can only detect single parameter.Adopt the electrochemical method test strip of enzymatic analysis and since have be easy to use, measure highly sensitive, can obtain advantage such as experimental result fast, obtained to use widely in family and hospital.The multiparameter integrating test bar that the present invention designs can be integrated in glucose, uric acid and cholesterol on the same base material, adopt the method for self-priming, by corresponding testing tool timesharing ground corresponding test event is carried out quantitative test then, can satisfy the clinical requirement that multiparameter is detected.
Summary of the invention
In order to remedy the limitation that existing strip can only carry out the single parameter test, the present invention is based on biological enzyme technology, a kind of whole blood test integrating test bar with multi-parameters test function is provided, and it can timesharing in conjunction with the relevant detection instrument or step by step physiological parameters such as blood sugar, uric acid or cholesterol is measured.
Technical scheme of the present invention is as follows:
Multi-parameter whole blood integrating test bar, it is characterized in that: described strip is based on the synthetic substrate of macromolecular material, covers conductive silver layer, conductive carbon layer on the substrate successively, insulation course, the non-drying glue layer that is formed by insullac and the covering rete of being made up of hydrophilic high molecular material;
It is right that described conductive silver layer comprises as many groups silver bar of electrode, and described silver bar is to the injection port rear end that is set in parallel in the substrate middle and upper part end to substrate;
Described conductive carbon layer is located at the right middle and upper part of silver bar accordingly, comprises that many group carbon bars are right;
Described insulation course covers the substrate middle and upper part, and the corresponding carbon bar that exposes is to the zone;
Described non-drying glue layer covers on the described insulation course accordingly, form each reaction tank zone, and expose injection port, and described each reaction tank bottom is provided with and is used for detecting the required biology enzyme of different blood constituents, and described each reaction tank is by same injection port sample introduction;
Described covering rete is positioned at and covers whole substrate middle and upper part on the non-drying glue layer, is used for sucking blood sample to described reaction tank from injection port.
Multi-parameter whole blood integrating test bar advantage of the present invention:
● it is located at glucose oxidase, urate oxidase or cholesterol oxidase respectively on the same base material, and by the same injection port sample introduction in strip side, but each reaction tank is separated from each other, and forms electrochemical reaction cell separately, does not disturb mutually during electrochemical reaction.
● it can take multiple measurements same parameter by in the middle of a plurality of reaction tanks the same enzyme being set.
Description of drawings
Fig. 1 is the overall diagram of multi-parameter whole blood integrating test bar;
Fig. 2 is the exploded view of the conductive silver layer of multi-parameter whole blood integrating test bar;
Fig. 3 is the exploded view of the conductive carbon layer of multi-parameter whole blood integrating test bar;
Fig. 4 is the exploded view of the insulation course of multi-parameter whole blood integrating test bar;
Fig. 5 is the exploded view of the non-drying glue layer of multi-parameter whole blood integrating test bar;
Fig. 6 is the exploded view of the covering rete of multi-parameter whole blood integrating test bar;
Fig. 7 has the design sketch of a plurality of multi-parameter whole blood integrating test bars when being used in combination.
Embodiment
Describe the present invention in detail below in conjunction with accompanying drawing.
Multi-parameter whole blood integrating test bar of the present invention is based on the synthetic substrate of macromolecular material, insulation course, non-drying glue layer that covers conductive silver layer, conductive carbon layer on the substrate successively, formed by insullac and the covering rete of being made up of hydrophilic high molecular material are referring to Fig. 1.The corresponding sample introduction oral-lateral of one side of substrate, opposite side is the electrode side.The concrete material of substrate can be selected various macromolecular materials for use, as Polyvinylchloride (PVC), polypropylene or polycarbonate etc.
Referring to Fig. 2, it is right that described conductive silver layer comprises as many groups silver bar of electrode, described silver bar is to the injection port rear end that the is set in parallel in substrate 1 middle and upper part end to substrate, silver bar 2 is to adopt screen printing technique typographic, it is used for transmitting the electronics that produces in the electrochemical reaction, thereby provides the detection electric current for testing circuit; The right adjacent silver bar of adjacent silver bar can link together, and can make the strip line succinct, compact conformation, and do not influence the detection of adjacent reaction pool chemical reaction signal.
Referring to Fig. 3, described conductive carbon layer is located at the right middle and upper part of silver bar accordingly, comprises that many group carbon bars are to 3.Described carbon bar is to adopt the screen printing technique printing to 3, mainly is to provide corresponding reaction media for electrochemical reaction.
Described insulation course 4 covers the substrate middle and upper part, and the corresponding carbon bar that exposes is to the zone; It is that operate as normal for strip provides corresponding protection, in order to avoid produce short circuit, and referring to Fig. 4.
Described non-drying glue layer 5 covers on the described insulation course 4 accordingly, form each reaction tank zone 8, and expose injection port 7, described each reaction tank bottom is provided with and is used for detecting the required biology enzyme of different blood constituents, described each reaction tank is by same injection port sample introduction, referring to Fig. 5, the insulation course between described each reaction tank and non-drying glue layer form blocking-up wall 9 reaction tank are separated, to reduce mutual interference.
Described covering rete 6 is made up of hydrophilic high molecular material, is positioned to cover whole substrate middle and upper part on the non-drying glue layer.Hydrophilic covering rete utilizes surface tension to suck blood sample to described reaction tank, referring to Fig. 6 from injection port.The thickness that covers rete 6 is 0.15mm, can select polyvinyl alcohol (PVA), polyethylene terephthalate (PET) or other hydrophilic material for use.
During detection, at first with the injection port end in contact blood of multi-parameter whole blood integrating test bar of the present invention, because the effect of hydrophilic coverlay forms siphonage, blood is inhaled in the reaction tank by injection port, and the blocking-up wall is separated each reaction tank mutually.Constant potential in chronological sequence order to be applied to each silver bar successively right, thereby the phase mutual interference of signal when avoiding testing.By detecting the size of electric signal, just can obtain the information of correlation parameter.
In actual applications, described biology enzyme can be the glucose oxidase that is used to detect glucose, be used to detect uric acid urate oxidase, be used for the cholesterol oxidase of cholesterol detection etc.Multi-parameter whole blood integrating test bar of the present invention is used in combination, can detects a plurality of different parameters or same parameter is carried out repeated detection, as shown in Figure 7.
Claims (6)
1. multi-parameter whole blood integrating test bar, it is characterized in that: described strip is based on the synthetic substrate of macromolecular material, covers conductive silver layer, conductive carbon layer on the substrate successively, insulation course, the non-drying glue layer that is formed by insullac and the covering rete of being made up of hydrophilic high molecular material;
It is right that described conductive silver layer comprises as many groups silver bar of electrode, and described silver bar is to the injection port rear end that is set in parallel in the substrate middle and upper part end to substrate;
Described conductive carbon layer is located at the right middle and upper part of silver bar accordingly, comprises that many group carbon bars are right;
Described insulation course covers the substrate middle and upper part, and the corresponding carbon bar that exposes is to the zone;
Described non-drying glue layer covers on the described insulation course accordingly, form each reaction tank zone, and expose injection port, and described each reaction tank bottom is provided with and is used for detecting the required biology enzyme of different blood constituents, and described each reaction tank is by same injection port sample introduction;
Described covering rete is positioned at and covers whole substrate middle and upper part on the non-drying glue layer, is used for sucking blood sample to described reaction tank from injection port.
2. multi-parameter whole blood integrating test bar according to claim 1 is characterized in that: insulation course between the described reaction tank and non-drying glue layer form the blocking-up wall reaction tank are separated, to reduce mutual interference.
3. multi-parameter whole blood integrating test bar according to claim 1 and 2 is characterized in that: the right adjacent silver bar of adjacent silver bar is joined together.
4. multi-parameter whole blood integrating test bar according to claim 1 is characterized in that: the macromolecular material that described substrate is adopted is Polyvinylchloride, polypropylene or polycarbonate.
5. multi-parameter whole blood integrating test bar according to claim 1 is characterized in that: the hydrophilic high molecular material that described covering rete adopts is polyvinyl alcohol (PVA) or polyethylene terephthalate.
6. multi-parameter whole blood integrating test bar according to claim 1 is characterized in that: described biology enzyme comprises detection glucose oxidase, urate oxidase or cholesterol oxidase.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510011615 CN1851459A (en) | 2005-04-22 | 2005-04-22 | Multi-parameter whole blood integrating test bar |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510011615 CN1851459A (en) | 2005-04-22 | 2005-04-22 | Multi-parameter whole blood integrating test bar |
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| CN1851459A true CN1851459A (en) | 2006-10-25 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010043075A1 (en) * | 2008-10-17 | 2010-04-22 | 红电医学科技股份有限公司 | Liquid test strip and the method |
| US8133718B2 (en) | 2008-10-17 | 2012-03-13 | Actherm Inc | Analytical strip and detecting method using the same |
| CN102539492A (en) * | 2010-12-27 | 2012-07-04 | 深圳光启高等理工研究院 | Information acquisition device and equipment based on Internet of things |
| US8367015B2 (en) | 2009-03-23 | 2013-02-05 | Actherm Inc | Analytical strip and the manufacturing method thereof |
| US8372660B2 (en) | 2008-10-09 | 2013-02-12 | Actherm Inc | Quantitative analyzing method |
| CN106353387A (en) * | 2016-08-24 | 2017-01-25 | 三诺生物传感股份有限公司 | Electrochemical testing strip and method for simultaneously testing various substances to be tested |
| CN110327057A (en) * | 2019-07-30 | 2019-10-15 | 三诺生物传感股份有限公司 | A kind of multiple determination strip |
| CN115684317A (en) * | 2022-10-28 | 2023-02-03 | 东莞市源凯电气有限公司 | Purine detection module and method |
-
2005
- 2005-04-22 CN CN 200510011615 patent/CN1851459A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8372660B2 (en) | 2008-10-09 | 2013-02-12 | Actherm Inc | Quantitative analyzing method |
| WO2010043075A1 (en) * | 2008-10-17 | 2010-04-22 | 红电医学科技股份有限公司 | Liquid test strip and the method |
| US7964370B2 (en) | 2008-10-17 | 2011-06-21 | Actherm Inc | Analytical strip and detecting method using the same |
| CN102171566A (en) * | 2008-10-17 | 2011-08-31 | 红电医学科技股份有限公司 | Liquid test strip and the method |
| US8133718B2 (en) | 2008-10-17 | 2012-03-13 | Actherm Inc | Analytical strip and detecting method using the same |
| US8367015B2 (en) | 2009-03-23 | 2013-02-05 | Actherm Inc | Analytical strip and the manufacturing method thereof |
| CN102539492A (en) * | 2010-12-27 | 2012-07-04 | 深圳光启高等理工研究院 | Information acquisition device and equipment based on Internet of things |
| CN106353387A (en) * | 2016-08-24 | 2017-01-25 | 三诺生物传感股份有限公司 | Electrochemical testing strip and method for simultaneously testing various substances to be tested |
| CN106353387B (en) * | 2016-08-24 | 2019-11-29 | 三诺生物传感股份有限公司 | A kind of electrochemical test bar and the method for detecting a variety of determinands simultaneously |
| CN110327057A (en) * | 2019-07-30 | 2019-10-15 | 三诺生物传感股份有限公司 | A kind of multiple determination strip |
| CN115684317A (en) * | 2022-10-28 | 2023-02-03 | 东莞市源凯电气有限公司 | Purine detection module and method |
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