CN1850065A - Oral use of conjugated linoleic acid and its derivatives for muscle building and skin beautification - Google Patents

Abstract

The invention relates to the use of Conjugated Linoleic Acid (CLA) and its derivatives for oral administration for building up body, reducing weight, nourishing skin and beautifying skin, and also relates to the use of Conjugated Linoleic Acid (CLA) and its derivatives for animal health care. The invention relates to the application of Conjugated Linoleic Acid (CLA) and derivatives thereof in preparing oral preparations for strengthening body, protecting skin, nourishing and beautifying; the preparation comprises tablet, soft capsule, granule, capsule and pill. The invention has the advantages that: conjugated Linoleic Acid (CLA) and its derivatives can enter skin and muscle tissue by oral administration, promote metabolism, and remove or replace melanin to achieve skin whitening and caring effects; or increasing the content of myoglycogen, myosin, actin and myoglobin, promoting the generation of muscle cells, and achieving the effect of increasing the muscle volume and the muscle fiber strength.

Description

Oral use of conjugated linoleic acid and its derivatives for muscle building and skin beautification

【Technical field】

The present invention relates to the use of conjugated linoleic acid (CLA) and its derivatives for oral use in muscle building, body building, weight loss and skin care and beauty. The present invention also relates to the use of conjugated linoleic acid (CLA) and its derivatives in animals Health care use. The present invention also relates to methods for the preparation of related formulations and other compositions.

【Background technique】

In modern society, due to the rapid development of science and technology, mechanization, electrification, automation, and penetration into various fields of human society, people's physical labor consumption has been greatly reduced, including housewives' daily essential housework, which has also been modernized. equipment replaced. People's daily physical exertion has dropped to less than one-tenth of what it was half a century ago.

On the other hand, with the rapid development of food culture, various delicious foods emerge in endlessly. Most people no longer worry about whether they can fill their stomachs. Tasting various delicious foods has become fashionable. Improve meals, talk about business, treat guests to dinner, and send nutritious food to sick people. Various factors urge people to eat too much food without knowing it.

Due to the reduction of physical exertion and excessive supplementation of nutrients in modern people, the energy metabolism of the human body is unbalanced, leading to a series of modern diseases such as hyperviscosity, hyperlipidemia, diabetes, hypertension, and coronary heart disease. Obesity is one of these modern diseases. The health killer in the 21st century—obesity has attracted more and more attention from the international medical community, so some experts pointed out that obesity has become three serious problems threatening human health together with smoking and AIDS.

In recent years, losing weight has become a fashion. But how can you lose weight in a healthy and effective way while still looking toned?

Some experts put forward a new concept of weight loss ----- nutritional weight loss! That is, good weight loss methods and excellent weight loss products are the only way to lose weight healthily. Choose good weight loss food, through balanced nutrition, reasonable diet, to achieve the effect of weight loss and fitness. Nutritional regulation and weight loss, a new concept, discard heavy fat and reshape yourself.

"No diarrhea, no dieting, no fatigue" are the three internationally accepted principles of healthy weight loss. Some weight-loss drugs are mixed with drugs that stimulate metabolism (some are illegal drugs, such as bupropion, etc.), and the purpose of reducing body weight is achieved by excreting and consuming body water and food that will not be digested and absorbed in the future. The harm is that it can cause collapse, abdominal pain, nausea, vomiting, electrolyte imbalance, malnutrition and other consequences. Although it can also temporarily lose weight, it loses not only fat, but also important substances such as water, minerals and protein, and the drug is discontinued. Then it will rebound and gain weight. The essence of dieting to lose weight is to excessively reduce energy intake. Its harm is the same as that of suppressing appetite. It can cause insomnia, dizziness, nausea, vomiting, malnutrition, and decreased immune function. Can lead to anorexia nervosa, severe cases are life-threatening. Both diarrhea and dieting can cause fatigue.

Therefore, it is necessary to seek a substance that can achieve the goal of weight loss by improving nutrition distribution, and does not produce side effects such as insomnia, dizziness, nausea, vomiting, malnutrition, and decreased immune function. At the same time, the synergistic effect of improving nutrient distribution is to increase muscle tissue, enhance the overall quality of the body, and achieve a healthy body.

Foreign studies have proved that conjugated linoleic acid in the body may reduce body fat by reducing fat deposition and increasing fat breakdown in fat cells. Foreign scholars have pointed out that CLA may inhibit the formation of adipocytes in two different ways, including inhibiting their proliferation and inhibiting their differentiation. The experimental results of Park et al. (Park Y. et al. Effect of conjugated linoleic acid on bodycomposition in mice [J]. Lipids, 1997, 32: 853-858) confirmed that conjugated linoleic acid reduces the physiology of mouse body fat The above mechanisms include inhibition of fat storage in adipocytes and acceleration of β-oxidation in skeletal muscle and increase in skeletal muscle mass. West et al found (West D.B.Conjugated linoleic acid rapidly reduces body fat content in mice withoutaffecting energy intake[J].Am.J.Physiol.1999; 276:R1172-R1179.), compared with the control without conjugated linoleic acid Rats fed a diet containing the CLA mixture had a 70 percent decrease in body fat percentage, while their body water, protein and ash percentages increased significantly compared to the control group.

Ha et al's research found that CLA has anticancer effect (Ha Y, et al.Inhibition of benz[a]pyrene-inducedmouse forestomach neoplasis by conjugated dienoic derivatives of linoleic acid[J].CancerRes., 1990,50:1097-1101 .), and accordingly infer that CLA is an antioxidant, and the subsequent CLA antioxidant performance test shows that CLA has stronger antioxidant performance than VE.

Someone co-cultured CLA with mouse skin cancer keratinocytes, and the results showed that: CLA can inhibit the production of PEG2 and AA that can be induced by TPA. CLA regulates the phospholipid composition of the cell membrane, protects the phospholipase of the cell membrane from being activated, and reduces the release of arachidonic acid to produce PEG2 (Liu KL, Belury MA. Conjugated linoleic acid reduces arachidonic acid content and PGE ₂ synthesis in murine keratinocytes. Cancer letters 1998; 127:15-22). Another study found that 9z, lle-CLA can specifically bind to peroxisome proliferator-activated receptor-α (PPAR-α) in the dermis, promote the differentiation of keratinocytes, and inhibit the carcinogenesis of skin tumors (Thuillier P, et al. Activators of peroxisome proliferator-activated receptor-α partially inhibit mouse skin tumor promotion. Molecular Carcinogenesis 2000;29:134-142. CLA was studied by measuring the synthesis of type I procollagen and its associated Derorin protein by fibroblasts in the dermis. Different combinations of CLA and EGCG were co-cultured with fibroblasts in human dermis. The results showed that both CLA and EGCG alone could increase the content of type I procollagen, and the combined use of the two had a synergistic effect. While type I procollagen The improvement of the Derorin protein content associated with it can smooth wrinkles, repair photodamaged skin, and show anti-aging effects. CLA has scavenging free radicals and anti-lipid peroxidation (WO0182861 Treatment of skin damage using conjugated linoleic acid and ascorbyl fatty acid esters), promote the growth of skin collagen cells, maintain the proliferation cycle of cells, prevent cells from developing in the direction of aging and differentiation, thereby exerting anti-aging and anti-inflammatory effects.

US6551602; US2004043963; US6403064; WO0182861 patents disclose the use of CLA in topical compositions and skin beauty care, such as anti-wrinkle, enhancing skin elasticity and thickness, repairing photodamaged skin, whitening effect, and moisturizing, nutrition, Anti-inflammatory and anti-allergic etc. It is disclosed in US6300374 that the zinc salt of conjugated linoleic acid can treat various skin diseases, such as eczema, psoriasis, dermatitis and the like. These studies or published patents suggest that topical CLA can nourish or care for bare skin, and we hypothesize that oral CLA can also reach the skin site to exert the same pharmacological effects.

Based on the above considerations, the applicant unexpectedly discovered through experiments that oral administration of conjugated linoleic acid (CLA) and its derivatives can improve nutrition distribution, lose weight and increase muscle, enhance immunity, and also have skin nutrition and beauty effects.

【Content of invention】

The object of the present invention is to orally administer conjugated linoleic acid (CLA) and its derivatives for muscle building, fitness, weight loss, skin nutrition and skin care, especially for muscle building (bodybuilding). The invention also relates to the use of conjugated linoleic acid (CLA) and its derivatives for animal health. The present invention also relates to methods for the preparation of related formulations and other compositions.

In fact, the present invention relates to the application of conjugated linoleic acid (CLA) and its derivatives in the preparation of oral preparations for muscle building, skin care, nutrition and cosmetic purposes;

As mentioned above, conjugated linoleic acid (CLA) and its derivatives orally enter the skin and muscle tissue to promote their metabolism, remove or replace melanin, and achieve the effect of whitening and skin rejuvenation; or increase muscle glycogen, myosin , the content of actin and myoglobin, promote the generation of muscle cells, and achieve the effect of increasing muscle volume and muscle fiber strength;

As above-mentioned preparation, its preparation comprises tablet, soft capsule, electuary, granule, capsule and pill;

The above-mentioned preparation, the preparation contains optional one or more commonly used antioxidant physiologically active substances;

CLA and derivatives thereof as described above, wherein CLA comprises one or more cis and trans form isomers of 9,11-10,12- and 11,13-octadecadienoic acid; CLA derived The substance is ethyl ester, triglyceride, calcium salt, zinc salt and other possible derivatives or salts of CLA;

The above CLA and its derivatives, characterized in that the amount of conjugated linoleic acid (CLA) and its derivatives is 0.01-20% (W/W);

As mentioned above, the commonly used antioxidant physiologically active substances are vitamin E or TBHQ or tea polyphenols or selenium (Se);

As mentioned above, the commonly used antioxidant physiologically active substances, in addition to protecting CLA and its derivatives to exert normal physiological functions, also play a role in synergistic scavenging of free radicals in the body, especially for muscle growth and more active substances after body activity is enhanced. There are many free radicals, so as to achieve the effect of cleaning the internal environment of the human body.

The invention adopts cell experiments and animal experiments to study the effects of conjugated linoleic acid (CLA) and its derivatives on reducing fat and increasing muscle, building body and nourishing skin.

The cell experiments used mouse 3T3-L1 preadipocytes. The 3T3-L1 cell line is a preadipocyte derived from mouse embryos and has a fibroblast morphology. It is currently an internationally recognized cell line for studying the process of adipogenicity. Under differentiation-inducing stimuli, 3T3-L1 preadipocytes differentiate into mature adipocytes. In this study, the in vitro culture experiment of adipocytes was used, and adipocytes were cultured and differentiated in the culture medium added with differentiation inducers. The experiment consisted of two test groups, the control group and the CLA group. The culture medium of the control group was cultured without adding other substances; the culture medium of the CLA group was cultured with CLA (concentration changed in a gradient), and the morphology was observed on the eighth day, and compared with the control group.

Results: Compared with the control group, the cells in the CLA group were obviously fibrous, and small oil droplets were scattered, indicating that it had a certain inhibitory effect on the proliferation of 3T3-L1 adipose precursor cells and the differentiation into adipocytes, suggesting that conjugated linoleic acid (CLA) has potential weight loss and lipid-lowering effects.

Animal experiments mainly studied the effects of conjugated linoleic acid (CLA) and its derivatives on improving body muscle composition and promoting the redistribution of nutrients to achieve weight loss and bodybuilding with firm muscles and uniform distribution of body muscles. At the same time, the skin of experimental animals was also studied. state. The test animals in the CLA group were significantly better than the control group: the skin gloss was better, and there were fewer abnormal spots or skin colors caused by skin diseases. These advantages of conjugated linoleic acid (CLA) and its derivatives are unmatched by the hot-selling slimming products on the market.

There were 2 test groups in the experiment: blank control group and conjugated linoleic acid calcium (conjugated linoleic acid calcium is the form of conjugated linoleic acid calcium salt, referred to as: CLA-Ca) group, each test group was set 2 There were 2 repetition groups, 5 pigs in each repetition group, a total of 20 test pigs (2♀, 3♂/replication). The body weight of the test pigs was measured before the test to ensure that the test pigs' body weight and body shape were not significantly different among each repeated group. The pre-test period is 5 days, and the formal test period is 64 days.

The dose of CLA-Ca powder was 6.7‰ (equivalent to pure CLA-Ca 5.0‰) directly into the powdered feed of pigs in the corresponding test group, stirred evenly, and fed continuously every day.

Determination of production performance indicators: Weigh the individual pigs recorded at the beginning and end of the experimental stage, record the feed intake of each repeated group, and calculate the average daily gain, feed-to-meat ratio, and daily feed intake.

In vivo fat measurement and grading adopt B-ultrasound instrument to carry out in-vivo fat measurement and grading at the animal test site at the beginning and end of the experimental stage. The testing items include B-ultrasound measurement of backfat thickness, eye muscle area, and estimation of carcass lean meat percentage.

After the test, 2 medium-sized pigs (1 each for ♀ and 1 ♂) were selected from each column of the blank group and the CLA-Ca group, a total of 8 pigs were slaughtered after 24 hours of fasting. "Procedures" method, measuring eye muscle area, backfat thickness, intermuscular fat, and calculating carcass lean meat percentage. Divide the lean meat, fat, skin, and bone of the left carcass and weigh them; measure the pH value of the longissimus dorsi muscle at the 13th to 14th intercostal space 45 minutes after slaughter, and evaluate the meat color and marbling grade. The experimental results are shown in Table 1-3: the results are detailed in Table 1-3:

Table 1 Effect of CLA calcium on weight gain and feed conversion efficiency of finishing pigs Initial weight(kg) Final weight(kg) Daily weight gain (g) feed conversion efficiency Daily feed intake (kg/d head) control group test group 59.25059.750 102.20098.550 681.75±10.10624.60±16.83 3.91±0.053.82±0.28 2.712.42

Table 2 Effect of CLA calcium on carcass traits Test indicators control group test group Test indicators control group test group Pre-mortem body weight (kg) carcass weight (kg) slaughter percentage (%) hind leg ratio (%) carcass lean meat percentage (%) 103.25±4.5576.04±3.1573.67±1.9829.17±1.6055.41±5.57 100.5±1.4773.32±3.0372.96±2.7830.90±1.9859.84±2.31 Head Weight (kg) Sebum Weight (kg) Bone Weight (kg) Hind Leg Weight (kg) Lean Weight (kg) 6.85±0.9510.04±2.226.52±0.2211.16±0.6220.54±1.93 6.19±0.488.90±1.565.63±0.8611.1±0.3521.66±0.80

Table 3 Effect of CLA calcium on carcass traits Test indicators control group test group Test indicators control group test group Fat thickness at the thickest part of the shoulder (mm) 6-7 rib fat thickness (mm) last rib fat thickness (mm) fat thickness at the front of the shoulder (mm) average fat thickness (mm) 41.18±7.2228.53±6.6815.58±6.0413.4±7.5624.68±6.77 33.2±8.8722.1±4.8315.4±5.1415.95±4.5121.68±5.07 B-ultrasound fat thickness (mm) carcass eye muscle area (cm ² ) B-ultrasound eye muscle area (cm ² ) estimated lean meat percentage (%) estimated lean daily gain (g/day.head) 21.35±9.6647.26±7.2637.84±1.7151.55±1.37205.55±11.72 15.05±4.3650.49±4.7440.08±2.4655.01±1.17212.33±4.82

The above experiments show that:

(1) Evaluation of skin condition and meat quality: the skin of the test group was fair and ruddy, the coat was neat and shiny, and there were less abnormal skin phenomena such as rashes and plaques caused by skin diseases, showing the improvement function of CLA on skin condition; In terms of meat quality evaluation, the meat color score of the experimental group was higher than that of the control group.

(2) Evaluation of overall body shape and muscle state: In terms of body shape, the fattening pigs in the test group were long in body, straight in back, firm and contracted in abdominal muscles, round and firm in hip muscles and evenly distributed, and in a strong state of activity. After slaughtering and dividing, it can be seen that the thickening of muscle fibers and the proliferation of longitudinal fissures in the fattening pigs in the test group increased the volume and weight of the muscles, which manifested as changes in the shape of the muscles, so the overall shape of the body was benignly developed.

(3) Effects on weight gain and feed conversion efficiency: Compared with the control group, the daily weight gain, average daily feed intake and feed-to-weight ratio of the experimental group were all reduced.

(4) Slaughter rate and carcass traits: the proportion of hind legs, carcass lean meat percentage, carcass eye muscle area and B-ultrasound eye muscle area in the experimental group increased, and the average fat thickness, B-ultrasonic fat thickness and relative sebum weight decreased compared with the control group up. The lean meat percentage of the test group was significantly higher than that of the control group.

When people breathe oxygen into the body, some of it is transformed into active free radicals, which will cause cell aging, increase wrinkles, memory loss, and weaken the immune system. Therefore, the intake of antioxidants can effectively scavenge active free radicals and delay the aging of the body and skin.

The invention adopts a pyrogallol autoxidation method to study the antioxidant effect of conjugated linoleic acid (CLA) and its derivatives.

Make a 6mmol/L solution of pyrogallol with dilute hydrochloric acid. Add 0.02% of the sample to 4.1 mL of pH 8.2 buffer and mix well. Take 0.1mL of pyrogallol solution and add it to the above mixed solution, and use the time program on the ultraviolet spectrophotometer to measure continuously at 320nm for 300s. The increase in absorbance express. The percentage inhibition rate of the sample pyrogallol autoxidation rate was calculated by formula (1-1).

The results are shown in Table 4.

In the formula, is the autoxidation rate of pyrogallol in the control group; is the autoxidation rate of pyrogallol when adding the sample. The experimental results are shown in Table 4:

Table 4 Inhibition rate of superoxide anion by CLA and its ethyl ester sample Inhibition rate(%) CLACLA Ethyl Ester Tocopherol 24.3221.637.21

The above results show that in the pyrogallol autoxidation system test, CLA and CLA ethyl ester of the present invention have a stronger superoxide anion scavenging effect than vitamin E at the same concentration, and are an effective antioxidant.

Conjugated linoleic acid (CLA) and derivatives thereof described in the present invention are used orally for weight loss, muscle gain and skin care and cosmetic purposes. 30 obese patients (volunteers) were randomly divided into two groups, and one group took 4.5 gCLA, and the other group received only a placebo containing nothing. After 3 months of food testing, it was found that after eating CLA, the subjects' waist circumference, abdominal circumference, and hip circumference decreased significantly, especially the waist circumference and abdominal circumference, and the thickness of subcutaneous fat in the human body also decreased significantly. The statistical results show that the weight loss rate of the placebo group is 0%; the body weight loss rate of the CLA group is 100%, with an average weight loss of 12.5%. Lose weight and increase muscle.

Compared with the existing products, the present invention has the following advantages: Conjugated linoleic acid (CLA) and its derivatives are taken orally into the skin and muscle tissue to promote their metabolism, remove or replace melanin, and achieve the effect of whitening and skin rejuvenation; The content of muscle glycogen, myosin, actin and myoglobin can promote the production of muscle cells and achieve the effect of increasing muscle volume and muscle fiber strength.

【Detailed ways】

Several specific embodiments of the present invention will be described below, but the content of the present invention is not limited thereto.

Example 1: CLA functional blend oil

(1) Mix 5g of CLA with sesame oil, olive oil or sunflower oil evenly to make 100g of blended oil, and obtain the finished product after packaging. Ways of eating: Add directly as cooking oil to salads or other seasonings for raw and cooked foods.

Example 2: Functional food containing conjugated linoleic acid and its derivatives

(1) CLA chocolate: Mix 1g of CLA with cocoa pulp, cocoa powder, cocoa butter, milk powder, edible oil, sugar, emulsifier and spices in a certain proportion to obtain 100g, use a rotating grinding roller to make it micronized, and use a fine grinder After fine grinding, it is processed into fine powder flakes with a rotating grinding roller, passed through a 60-mesh sieve, and finally shaped by a molding machine press.

(2) CLA-Ca candy: Put white granulated sugar in an aluminum pot, add a little water, and cook until it becomes silky and not sticky, then lower the temperature to 40°C, add 5g of CLA calcium powder, and make 100g. turn off the flame. Pour the sugar into an enamel plate coated with edible oil, wait for it to cool slightly, and cut the sugar into small pieces with a knife.

(3) Preparation of pastry sugar powder containing CLA: Mix 10 g of CLA and pastry sugar powder to obtain 100 g, stir, grind, pass through a 60-mesh sieve, and pack separately to obtain the finished product.

Example 3: Nutritional supplements containing conjugated linoleic acid and its derivatives

(1) Mixture containing CLA-Ca: Mix 3g of CLA-Ca powder with sucrose, inosine, catechol, and sodium benzoate in a certain proportion, dissolve in 100mL of distilled water, stir well to make it completely dissolved. Packed in cans, that is. Directions for use: Add to milk or other sports drinks and food.

(2) Preparation of effervescent tablets containing CLA: get 5g of CLA and mix them uniformly with citric acid, fumaric acid, and sodium bicarbonate in a certain proportion, make a soft material with ethanol, pass through a 60-mesh sieve, and granulate. Dry at ℃, granulate, add low-substituted hydroxypropyl cellulose and magnesium stearate, mix thoroughly to obtain 100g, punch out tablets with 12mm dimples, and obtain. Directions for use: Add to milk or other sports drinks and food.

Example 4: Health care products and pharmaceutical raw materials containing conjugated linoleic acid and its derivatives

(1) Preparation of hard capsules containing CLA calcium salt: get 5g of CLA calcium salt, mix it with vitamin E, sucrose, and starch in a certain proportion to make 100g, after crushing, pass through a 100-mesh sieve, and pack into hard capsules.

(2) Preparation of CLA-containing soft capsules: gelatin, glycerin, and distilled water are mixed in a ratio of 40:12:48 to form a glue solution, and the glue solution and CLA are passed through the double holes of the dropping pill machine and dripped out at different speeds. Solidify in the cooled liquid paraffin, the dosage ratio of the three is 1.12:0.9:0.86. The obtained capsules are dried at about 20°C to obtain. Dosage: Take orally, take with warm water or milk.

(3) Conjugated linoleic acid microcapsules: Dissolve 3g of CLA, ethyl cellulose, starch, talcum powder, glycerin stearate, vitamin E, etc. in 100ml of ethanol in a certain proportion, mix well, and place in a spray dryer Spray drying, that is. Usage: used as raw material for medicine and food production.

(4) Prepare the oral instant tablet containing CLA: get 5g CLA and polysaccharide, gelatin, polypeptide, talcum powder, ethanol, coloring agent etc. are made into suspension in certain proportion, quantitatively subpackage in certain mould, freeze into solid state, then The temperature was raised under reduced pressure, and water was removed by sublimation to obtain 100 g of a high-porosity solid preparation. How to take: Take it without water, it dissolves quickly when it encounters saliva in the mouth.

(5) Preparation of buccal tablets containing CLA: mix lactose, sugar powder, and 17% starch slurry evenly in a certain proportion, pass through a 60-mesh sieve, and dry the wet granules in a drying oven (60°C-80°C) to obtain blank granules . Take 5g of CLA and mix it with blank granules to obtain 100g, and press it into tablets with a tablet machine. How to take: Take it sublingually.

(6) Preparation of CLA-containing microcapsules: dissolve ethyl cellulose (EC) in acetone, add 2 g of CLA and 0.7 g of talc, stir, and add the resulting suspension to liquid paraffin containing Span, and the system The temperature was gradually raised to 35°C, kept stirring, then raised to 57°C, stirred, filtered under reduced pressure, the obtained solid was washed 3 times with n-hexane, drained and dried to obtain 100 g of microcapsules.

(7) Preparation of nutrient solution containing CLA ethyl ester: mix 2g of CLA ethyl ester with honey, sucrose ester, vitamin E, selenium, protein sugar, and sodium dehydroacetate in a certain proportion, add water to 100ml, stir evenly, and then After bottling, sealing, and sterilization, the finished product is obtained.

Example 5: Animal Food and Feed Containing Conjugated Linoleic Acid and Its Derivatives

(1) Add directly to animal food and feed: Mix 5g of CLA calcium powder with 95g of dog food or pig feed, and stir evenly.

(2) Raw material additives for the production of pet food: take 0.05g of CLA and mix it with chicken breast meat, sorbitol, red yeast rice, vitamin E, potassium sorbate, sodium saccharin, and sodium nitrite in a certain proportion to form 100g, and control the mixture Degreasing with water, freezing and slicing, drying, packing, and it is ready.

(3) Raw material additives for the production of animal feed: remove impurities from corn, soybean meal, and bran, bake at 90°C for 18 hours, roughly mix them in a ratio of 10:3:2, crush them with a ball mushroom machine and pass through an 80-mesh sieve, and take Mix 95g of sieved powder with 5g of CLA calcium powder evenly, pack them into clean brown bottles, fill them with nitrogen gas, seal them, and irradiate them with the isotope cobalt 60 at a dose of 1.5 Mrad for long-term storage.

Claims (8)

1, conjugated linoleic acid (CLA) and derivant thereof are used for the application of the oral formulations of increasing muscule and skin-care nutrient, beautifying use in preparation.

2, application according to claim 1, oral skin and the muscular tissue of entering of conjugated linoleic acid (CLA) and derivant thereof promotes its metabolism, removes or displacement melanin, reaches the effect of whitening and softening skin; Or the content of increase muscle glycogen, myosin, actin and Myoglobin, promote muscle cell to generate, reach the effect that increases muscle volume and muscle fiber intensity.

3, preparation according to claim 1, its preparation comprises tablet, soft capsule, electuary, granule, capsule and pill.

4, according to claim 2 or 3 described preparations, preparation contains one or more optional tool antioxidation biological active substanceies commonly used.

5, CLA according to claim 1 and derivant thereof, wherein CLA comprise one or more 9,11-10,12-and 11, the cis of 13-octadecadienoic acid and trans forms isomer; The CLA derivant is ethyl ester, the triglyceride of CLA, calcium salt, zinc salt and other possible derivant or salt.

6, according to the CLA and the derivant thereof of claim 1 or 5, the consumption that it is characterized in that conjugated linoleic acid (CLA) and derivant thereof is 0.01-20% (W/W).

7, according to the described preparation of claim 4, tool antioxidation biological active substances commonly used is vitamin E or TBHQ or tea polyphenols or selenium (Se).

8, according to the described preparation of claim 7; tool antioxidation biological active substances commonly used; except protection CLA and derivant thereof are brought into normal play the physiological function; also play a part the collaborative interior free yl of removing; increase at muscle especially; the movable back that strengthens of human body produces more freedom base situation, reaches the effect of environment in the cleaning human body.