CN112674208A - Application of trans-10 cis-12 conjugated linoleic acid in enhancing skeletal muscle exercise endurance - Google Patents
Application of trans-10 cis-12 conjugated linoleic acid in enhancing skeletal muscle exercise endurance Download PDFInfo
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Abstract
本发明属于骨骼肌运动技术领域，公开了一种反10顺12共轭亚油酸在增强骨骼肌运动耐力中的应用。本发明提供了反10顺12共轭亚油酸的新用途，反10顺12共轭亚油酸可以显著增加动物的跑步运动时间和距离，同时增加动物骨骼肌的收缩持续时间，增强骨骼肌的运动耐力。反10顺12共轭亚油酸通过增强动物骨骼肌线粒体呼吸和骨骼肌氧化代谢相关酶活，同时提高骨骼肌I型纤维类型，从而显著增强骨骼肌的运动耐力。本发明中反10顺12共轭亚油酸不含对动物或人体有害的物质，添加量较少，可以直接加入动物饲料、食品或药品中，具有安全、有效、不会引起毒副作用等优点，在增强骨骼肌运动耐力方面的应用具有良好的推广应用前景。
The invention belongs to the technical field of skeletal muscle exercise, and discloses the application of trans-10cis-12 conjugated linoleic acid in enhancing exercise endurance of skeletal muscle. The invention provides a new application of trans-10cis-12 conjugated linoleic acid, and trans-10cis-12 conjugated linoleic acid can significantly increase the running time and distance of animals, and at the same time increase the duration of animal skeletal muscle contraction, and strengthen skeletal muscle exercise endurance. Trans-10cis-12-conjugated linoleic acid significantly enhanced the exercise endurance of skeletal muscle by enhancing mitochondrial respiration and oxidative metabolism-related enzyme activities of skeletal muscle, and at the same time increasing the type I fiber type of skeletal muscle. The trans-10cis-12-conjugated linoleic acid in the present invention does not contain substances harmful to animals or human body, and the added amount is small, and can be directly added to animal feed, food or medicine, and has the advantages of safety, effectiveness, and no toxic and side effects. , the application in enhancing skeletal muscle exercise endurance has a good promotion and application prospect.
Description
Technical Field
The invention belongs to the technical field of skeletal muscle movement, and particularly relates to application of trans-10 cis-12 conjugated linoleic acid in enhancing skeletal muscle movement endurance.
Background
Currently, skeletal muscle accounts for about 40-60% of the body weight of an adult animal, and is a striated muscle in muscle tissue, which is composed of thousands of muscle cells with contractile capacity, which are called muscle fibers. During development, myotubes are formed from the fusion of mesodermal progenitor cells, which redifferentiate to form myofibers. Normally, the number of muscle fibers is stable before the animal is born, and the growth of muscle in the postnatal stage is mainly reflected by changes in the size and type of muscle fibers, while the conversion of muscle fiber type is carried out throughout the growth and development process. The muscle fibers can be divided into different categories according to different characteristics. In daily life, the muscle fiber type is closely related to the motor ability of the body; in the process of animal production, the composition and difference of muscle fiber types are one of important factors influencing meat quality, and with the continuous expansion of livestock breeding scale, the living standard and consumption demand of people are increasingly improved, so that the people put forward higher and higher requirements on the market when selecting meat consumption products. Therefore, how to regulate the muscle fiber type and improve the exercise capacity and the meat quality has important significance for animal production, human body exercise and health.
The muscle fiber type of skeletal muscle is regulated and responded to a plurality of factors inside and outside the animal body during the growth process, so that the conversion between the fiber types is generated. The skeletal muscle is the most important movement and energy metabolism tissue in the animal body, and the transformation of fiber types in the skeletal muscle is finally reflected in the change of the contraction movement capability and the capability metabolism characteristic of the muscle, and the change not only reflects the physiological response of the animal body to factors inside and outside the animal body, but also reflects the living environment characteristic of the animal. In animal husbandry, factors affecting myofiber type conversion mainly include breed, age, sex, location, genetics, nutrition, feeding mode, and the like. Some nutrients can change the muscle fiber type and skeletal muscle motor ability by changing muscle metabolism, but the intrinsic mechanism is not clear, and the influence of a single component on the conversion of the muscle fiber type is reported to be less.
Conjugated linoleic acid is used as a nutrient, is almost a Wanlingdan for preventing modern civilization diseases in European and American health food at present, and is almost an indispensable health food for modern people living in twenty-first century from cancer resistance to cardiovascular diseases and diabetes prevention to weight control. Conjugated linoleic acid is formed in the process that rumen bacteria hydrogenate linoleic acid to stearic acid, and due to the special biological activity of the conjugated linoleic acid, the conjugated linoleic acid has attracted great attention in research of nearly twenty years, mainly showing aspects of resisting cancers, resisting obesity, resisting inflammation, resisting diabetes, promoting bone formation and the like. The double bonds of the conjugated linoleic acid have various position arrangement modes on the carbon chain, wherein each double bond has cis-form conformation and trans-form conformation, so the conjugated linoleic acid has abundant types, and the biological activity and the physiological function of different isomers are different. There are two major biologically active isomers that have been demonstrated, cis 9 trans 11 and trans 10, cis 12. However, at present, researches on the aspects of enhancing the skeletal muscle exercise endurance and the like by the conjugated linoleic acid, particularly on the aspects of mitochondrial respiration, skeletal muscle metabolism, muscle fiber type transformation and the like are blank, and no related technical report exists, so that the further popularization and application of the conjugated linoleic acid are influenced.
Through the above analysis, the problems and defects of the prior art are as follows: at present, researches on the aspects of enhancing the exercise endurance of skeletal muscles by conjugated linoleic acid and the like, particularly on the aspects of mitochondrial respiration, skeletal muscle metabolism, muscle fiber type conversion and the like are blank, and no related technical report exists, so that the further popularization and application of the conjugated linoleic acid are influenced.
The difficulty in solving the above problems and defects is: the effects of the trans-10, cis-12 conjugated linoleic acid on various aspects such as the whole exercise endurance, the external contraction duration of skeletal muscles, the type conversion of skeletal muscle fibers, the metabolic characteristics of skeletal muscle cells and the like are researched from different levels of animal living bodies, skeletal muscle tissues, cells and the like, so that the effect of the trans-10, cis-12 conjugated linoleic acid in enhancing the exercise endurance of the skeletal muscles is clarified, and a sufficient scientific basis is provided for the application of the trans-10, cis-12 conjugated linoleic acid. The solution to the above problems is difficult in the aspects of research ideas, research methods, technical means and the like.
The significance of solving the problems and the defects is as follows: the regulation and control function of the trans-10, cis-12 conjugated linoleic acid in enhancing the exercise endurance of skeletal muscles is clarified, and the application is popularized. On one hand, the feed can provide nutrition reference for improving the athletic ability of people, and on the other hand, the feed can provide a nutrition regulation and control scheme for the transformation of the types of the skeletal muscle fibers of livestock and poultry so as to improve the quality of livestock and poultry meat.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an application of trans-10 cis-12 conjugated linoleic acid in enhancing the exercise endurance of skeletal muscles, and particularly relates to an application of trans-10 cis-12 conjugated linoleic acid in changing metabolism-related enzyme activity, increasing mitochondrial metabolism of skeletal muscles and changing fiber types of the skeletal muscles so as to enhance the exercise endurance of the skeletal muscles.
The invention is realized in such a way that the application of trans-10 cis-12 conjugated linoleic acid in enhancing the exercise endurance of skeletal muscles; the addition amount of trans 10 cis 12 conjugated linoleic acid is 1%.
It is another object of the present invention to provide a use of trans 10 cis 12 conjugated linoleic acid for increasing the duration of contraction in vitro of a skeletal muscle.
Another object of the present invention is to provide a use of trans 10 cis 12 conjugated linoleic acid for increasing the content and proportion of type I muscle fibers in skeletal muscle.
Further, the adding proportion of the trans-10 cis-12 conjugated linoleic acid is 1%.
Another objective of the invention is to provide an application of trans-10 cis-12 conjugated linoleic acid in increasing skeletal muscle mitochondrial metabolism and changing enzyme activity related to skeletal muscle oxidative metabolism.
Further, the adding proportion of the trans-10 cis-12 conjugated linoleic acid is 1%.
It is another object of the present invention to provide a use of trans 10 cis 12 conjugated linoleic acid for modifying skeletal muscle fiber type.
By combining all the technical schemes, the invention has the advantages and positive effects that: the trans-10 cis-12 conjugated linoleic acid provided by the invention can obviously increase the running exercise time and distance of animals, and simultaneously increase the contraction duration of skeletal muscles of the animals, and enhance the exercise endurance of the skeletal muscles. Specifically, the trans-10 cis-12 conjugated linoleic acid can enhance the activity of related enzymes of mitochondrial respiration and oxidative metabolism of skeletal muscle of animals, and simultaneously improve the I-type fiber of the skeletal muscle, thereby obviously enhancing the exercise endurance of the skeletal muscle. Moreover, the trans-10 cis-12 conjugated linoleic acid does not contain substances harmful to animals or human bodies, has small addition amount, can be directly added into animal feed, food or medicines, has the advantages of safety, effectiveness, no toxic or side effect and the like, and has good popularization and application prospect in the aspect of enhancing the exercise endurance of skeletal muscles.
The invention can improve the quality of animal skeletal muscle, increase the exercise capacity of the animal and overcome the application defects of the conjugated linoleic acid monomer, provides the application of the conjugated linoleic acid in improving the quality of animal carcasses, widens the application range of the conjugated linoleic acid, obviously improves the production performance of the animal, is a technology for improving the exercise endurance and the meat quality of the animal skeletal muscle by a nutrition regulation and control means, has important significance for animal production and human body exercise health, and provides a powerful technical theoretical basis for the application of the conjugated linoleic acid in improving the exercise capacity and the animal production performance.
Research shows that the addition of trans-10 cis-12 conjugated linoleic acid into grains can obviously improve the exercise time and distance of mice, the external contraction duration of skeletal muscles, the proportion of I-type muscle fibers in the skeletal muscles and the oxidative metabolism capability of the skeletal muscles, thereby enhancing the exercise endurance of the skeletal muscles. Meanwhile, the trans-10 cis-12 conjugated linoleic acid is applied to the aspect of improving the exercise endurance of animals and human beings.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1A is a statistical graph of time and distance for endurance running exercises performed by different groups of mice provided by the embodiments of the present invention.
FIG. 1B is a graph showing the induction of contraction tension and the statistical distribution of muscle tension half-life of gastrocnemius muscles in different groups of mice under electrical stimulation conditions, according to an embodiment of the present invention.
FIG. 2A is a graph showing immunofluorescence staining of gastrocnemius type I muscle fibers and muscle cell membranes of various groups of mice according to an embodiment of the present invention.
FIG. 2B is the results and statistics of the protein expression of type I and type IIa muscle fibers in gastrocnemius muscle of different groups of mice according to the present invention.
Fig. 3A is a diagram illustrating the basal respiratory rate of gastrocnemius mitochondria of different groups of mice according to an embodiment of the present invention.
FIG. 3B is a graph showing the ATP content in gastrocnemius muscle of different groups of mice according to the present invention.
FIG. 3C is a graph showing the activity of gastrocnemius hexokinase in different groups of mice, provided by an example of the present invention.
FIG. 3D is a graph showing the activity of gastrocnemius succinate dehydrogenase in different groups of mice, according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In view of the problems of the prior art, the present invention provides a use of trans 10 cis 12 conjugated linoleic acid for enhancing endurance of skeletal muscle movement, which is described in detail below with reference to the accompanying drawings and examples.
The invention provides a new application of trans-10 cis-12 conjugated linoleic acid, and the addition amount of the trans-10 cis-12 conjugated linoleic acid is 1 percent; the trans-10 cis-12 conjugated linoleic acid can obviously improve the external contraction duration of skeletal muscle, the proportion of I-type muscle fiber in the skeletal muscle and the oxidative metabolism capability of the skeletal muscle, thereby enhancing the exercise endurance of the skeletal muscle.
Example 1: 1% of trans-10 cis-12 conjugated linoleic acid is added into the daily ration to increase running exercise time and distance of mice
1. To study the effect of trans 10 cis 12 conjugated linoleic acid monomer on skeletal muscle motor endurance in mice, male C57BL/6 mice at 8 weeks of age were used as subjects.
2. Experimental methods
(1) The animal feeding method comprises the following steps: 1, culturing alone per box; raising temperature and humidity: 22-26 ℃ and 40-70 percent; the method adopts the steps of 12 h: 12h day and night intermittent illumination; the feeding condition is kept stable all the time to ensure the reliability of the test result. During the raising period, the animals freely eat drinking water, and the feed and the drinking water are provided by the medical experimental animal center of Guangdong province. Purchased SPF grade C57BL/6 mice were pre-fed for 7 d. During which the animals were examined once daily.
(2) Animal grouping and treatment: mice of 8 weeks of age were randomly divided into 3 groups of 8 mice each, namely, a control group, a cis 9 trans 11 conjugated linoleic acid group and a trans 10 cis 12 conjugated linoleic acid group. The control group mice were fed normal diet, the cis 9 trans 11 conjugated linoleic acid group was fed normal diet supplemented with 1% of cis 9 trans 11 conjugated linoleic acid, and the trans 10 cis 12 conjugated linoleic acid group was fed normal diet supplemented with 1% of trans 10 cis 12 conjugated linoleic acid, and the experiment was carried out for 8 weeks.
(3) Sampling and detecting: at the 6 th week of the experiment, the endurance exercise capacity of the mice was examined using a mouse treadmill. The mice are anesthetized before sampling, gastrocnemius muscles are exposed, and electrical stimulation is carried out to detect the contraction capacity and the anti-fatigue capacity of the gastrocnemius muscles of different groups of mice.
3. The results are shown in FIG. 1:
FIG. 1 is a graph of the effect of different conjugated linoleic acid monomers on skeletal muscle exercise endurance in mice;
(1) as shown in fig. 1A, the addition of cis 9 trans 11 conjugated linoleic acid to the ration had no significant effect on the time and distance of the running of the mice on the treadmill compared to the normal ration group (control group), while the addition of trans 10 cis 12 conjugated linoleic acid to the ration significantly increased the running time and distance of the mice.
(2) The results are shown in fig. 1B, compared with the control group, the addition of cis 9 trans 11 conjugated linoleic acid to the ration has no significant effect on the anti-fatigue capability of the gastrocnemius muscle of the mice, while the addition of trans 10 cis 12 conjugated linoleic acid to the ration can significantly increase the anti-fatigue capability of the gastrocnemius muscle of the mice.
Example 2: 1 percent of trans-10 cis-12 conjugated linoleic acid is added into daily ration to increase the content and proportion of I-type muscle fibers in gastrocnemius of mice
1. To study the effect of trans 10 cis 12 conjugated linoleic acid on mouse muscle fiber type, 8 week old male C57BL/6 mice were used as subjects.
2. Experimental methods
(1) The animal feeding method comprises the following steps: the same as in example 1.
(2) Animal grouping and treatment: the same as in example 1.
(3) Sampling and detecting: the experiment was carried out for 16 weeks, on the day of completion of the experiment, the mice were sacrificed by cervical dislocation, gastrocnemius muscles of all mice were collected, sections were subjected to immunofluorescent staining, and proteins were extracted for Western Blot experiment. And detecting the influence of the addition of the conjugated linoleic acid to the daily ration on the mouse muscle fiber type.
3. The results are shown in FIG. 2:
FIG. 2 is a graph of the effect of different conjugated linoleic acid monomers on the type of gastrocnemius muscle fibers in mice;
(1) as shown in fig. 2A, the addition of cis 9 trans 11 conjugated linoleic acid to the ration had no significant effect on the content of mouse type I muscle fibers, compared to the normal ration group (control group), while the addition of trans 10 cis 12 conjugated linoleic acid to the ration significantly increased the content and proportion of mouse type I muscle fibers.
(2) As shown in fig. 2B, the addition of cis 9 trans 11 conjugated linoleic acid to the ration had no significant effect on the protein expression of mouse type I and type IIa muscle fibers compared to the normal ration group (control group), while the addition of trans 10 cis 12 conjugated linoleic acid to the ration significantly increased the protein expression of mouse type I and type IIa muscle fibers. The above results suggest that trans 10 cis 12 conjugated linoleic acid can increase the expression of oxidized muscle fibers of gastrocnemius in mice.
Example 3: 1% of trans-10 cis-12 conjugated linoleic acid is added into daily ration to improve the activity of related enzymes of calf muscle mitochondrial respiration and oxidative metabolism of mice
1. In order to study the influence of 1% trans-10 cis-12 conjugated linoleic acid added into daily ration on the mitochondrial respiration and oxidative metabolism related enzyme activity of calf muscles, an 8-week-old male C57BL/6 mouse is taken as a study object in the experiment.
2. Experimental methods
(1) The animal feeding method comprises the following steps: the same as in example 1.
(2) Animal grouping and treatment: the same as in example 1.
(3) Sampling and detecting: the experiment was carried out for 16 weeks, on the day of completion, the mice were sacrificed by dislocation of the cervical vertebrae, blood was taken from the eyeballs, gastrocnemius muscles were separated, and the supernatant was homogenized and taken to detect mitochondrial respiration. The kit is used for detecting the influence of the addition of 1% of conjugated linoleic acid to the rat gastrocnemius ATP content and the oxidative metabolism related enzyme activity.
3. The results are shown in FIG. 3:
FIG. 3 is a graph of the effect of different conjugated linoleic acid monomers on mitochondrial respiration and associated enzyme activity in the gastrocnemius muscle of mice;
(1) as shown in fig. 3A, 3B, 3C and 3D, compared to the control group, the addition of cis 9 trans 11 conjugated linoleic acid to the diet did not significantly affect the basal respiration, ATP content, succinate dehydrogenase activity and hexokinase activity of the gastrocnemius muscle mitochondria of the mouse, while the addition of trans 10 cis 12 conjugated linoleic acid to the diet significantly increased the activity of the hexokinase of the gastrocnemius muscle of the mouse, with a tendency to increase the basal respiration, ATP content and succinate dehydrogenase activity of the gastrocnemius muscle mitochondria of the mouse.
The addition of the trans-10 cis-12 conjugated linoleic acid into the daily ration can obviously improve the exercise time and distance of mice, the external contraction duration of skeletal muscles, the proportion of I-type muscle fibers in the skeletal muscles and the oxidative metabolic capacity of the skeletal muscles, thereby enhancing the exercise endurance of the skeletal muscles.
The trans 10 cis 12 conjugated linoleic acid is used in raising animal's and human's exercise tolerance.
In example 1, the trans 10 cis 12 conjugated linoleic acid can improve the exercise time and the exercise distance of a mouse living body and increase the contraction duration of isolated gastrocnemius muscle, and is the application of the trans 10 cis 12 conjugated linoleic acid in improving the exercise endurance of animals.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (7)
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| CN113455588A (en) * | 2021-06-15 | 2021-10-01 | 浙江大学 | Novel application of DHA (docosahexaenoic acid) as feed additive in regulating muscle fiber types |
| CN114097938A (en) * | 2021-10-27 | 2022-03-01 | 浙江大学 | Feed for promoting muscle injury repair of mice and application |
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| CN113455588A (en) * | 2021-06-15 | 2021-10-01 | 浙江大学 | Novel application of DHA (docosahexaenoic acid) as feed additive in regulating muscle fiber types |
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