CN1690690A - Biochip detection method with light intensity real-time detection and detection system thereof - Google Patents

Abstract

A biochip detection method with real-time detection of light intensity and its detection system. An optical density disk is added to the excitation light path to adjust the light intensity. A light detection system is installed under the slide table to detect The light intensity of the light reaching the sample to be detected, this light intensity value truly reflects the real situation of the light intensity of the excitation light source, this light intensity value is recorded in the computer, when the light intensity value changes, the computer system according to The signal provided by the detection circuit is judged, and measures are taken in time to adjust the optical density disc or the exposure of the sample to ensure the authenticity and reliability of the detection data. The present invention can not only adjust the light intensity through the optical density disk, but also adjust the exposure of the biological sample, so that the detection error caused by the difference in the light intensity or uniformity of the excitation light source in the detection of the biochip can be avoided, ensuring This ensures that the detection data of the biochip detection system is true and reliable.

Description

Biochip detection method and detection system with real-time detection of light intensity

Technical field

The invention relates to a biochip detection method with real-time detection of light intensity and a detection system thereof.

technical background

In the detection process of the biochip detection system, due to factors such as the service life of the xenon lamp of the excitation light source and the instability of the power grid voltage, the light intensity and uniformity of the xenon lamp light source often change, which will not only make the biochip detection system in the detection During the process, due to the change of the light intensity of the light source and the difference in uniformity, the detection data of the biochip detection system will be different, resulting in judgment errors, and photobleaching will also occur, causing damage to the biochip. At present, there are a variety of biochip detectors at home and abroad, but no real-time detection technology and adjustment measures for light intensity are used in these instruments to solve the impact caused by changes in light intensity of the light source.

Contents of the invention

The technical problem of the present invention is: in order to overcome the detection error problem caused by the light intensity difference of the light source in the detection process of the biochip detection system, a biochip detection system with real-time detection of light intensity is provided, so that the biochip detection When the light intensity of the light source changes during the detection process, the system can be detected in time, and the computer can take timely measures to adjust the optical density disc or control the exposure of the sample, so that the detection data is true and reliable.

The technical solution adopted by the present invention to solve its technical problems is: a biochip detection method with light intensity real-time detection, characterized in that: an optical density disc is added to the excitation light path of the biochip detection system to adjust the light intensity, A light detection system is installed under the loading stage, its purpose is to detect the light intensity of the light emitted by the xenon light source and reaching the sample to be tested. This light intensity value truly reflects the real situation of the excitation light source light intensity. This light intensity value It is recorded in the computer. When the light intensity value changes, the computer system judges according to the signal provided by the detection circuit, and takes timely measures to adjust the optical density disc or the exposure of the sample to ensure the authenticity and reliability of the detection data.

A biochip detection system with real-time detection of light intensity designed to realize the above method, including an optical system, an emission interference filter, a computer, a slide stage, a xenon lamp light source, and an excitation interference filter, is characterized in that: after the xenon lamp source There is an optical density disk for adjusting the light intensity, and a photodetector is installed under the slide table. The light emitted by the xenon lamp passes through the optical density disk, and then irradiates the slide table after passing through the excitation interference filter. The light intensity detector The optical signal can be detected, amplified by the integrated operational amplifier, and then sent to the computer for processing, and then the computer controls the adjustment of the optical density disc or the exposure of the sample.

The principle of the present invention is: the biological chip detection system can detect the light intensity and uniformity of the xenon lamp excitation light source on-line through the internal light intensity detection system, and the computer performs related control according to the detected light intensity value, which can be achieved through The optical density disc is used to adjust the light intensity, and also adjust the exposure of the biological sample to reduce the detection error caused by the difference of the excitation light source in the detection system of the biochip detection system.

The beneficial effect of the present invention is that the biochip detection system with light intensity detection can timely and effectively detect whether the xenon lamp light source is working normally and whether the light intensity is uniform, and can be conveniently controlled and adjusted by a computer, and the light intensity detection part circuit The required current is very small and does not affect the main power supply. The circuit only uses photodetectors and integrated op-amp devices, resistors, capacitors, etc. The structure of the optical density disc is simple, easy to install and adjust, economical, safe and reliable.

Description of drawings

Fig. 1 is a schematic diagram of the system of the present invention.

Detailed ways

As shown in Figure 1, the embodiment of the present invention consists of an image acquisition system 1, an optical system 2, an emission interference filter 3, a computer 4, a slide table 5, a light detector 6, an integrated operational amplifier 7, a xenon lamp light source 8, The optical density disk 9 and the excitation interference filter 10 are composed. Turn on the power supply of the xenon lamp, the light emitted by the xenon lamp 8 passes through the optical density disc 9, and then irradiates on the slide stage 5 after passing through the excitation interference filter 10. At this time, the light intensity detector 6 installed under the slide stage 5 can The optical signal is detected, and after the signal is amplified by the operational amplifier 7, it is sent to the computer 4, and the computer 4 processes it in time according to the received light intensity data, adjusts the optical density disk 9 or adjusts the exposure of the sample to eliminate the light source Detection data errors caused by changes in light intensity. During detection, after the sample placed on the slide stage 5 is excited by the excitation light, the fluorescence generated by the target molecules marked with fluorescent dyes on the sample under the excitation of monochromatic light enters the optical system 2 after passing through the emission narrow-band interference filter 3 The optical system 2 sends the captured image signal to the data acquisition system 1, and the data acquisition system 1 converts the image signal captured by the optical system 2 into a digital signal and sends it to the computer 4, and then the computer 4 performs relevant detection data processing.

The optical density disc 9 is made of coated optical devices, and its light transmission and filtration is uniformly distributed in the range of 99% to 5% in the range of 0° to 360°. The optical density disc can be driven by a motor to rotate. When the light intensity of the excitation light source changes, the computer can automatically control the motor to drive the optical density disc to rotate to the required position according to the detected light intensity signal to adjust the incidence of the excitation light. The computer can also change the exposure time setting to adjust the exposure of the sample according to the light intensity. The added light intensity detection circuit has few components, and the photodetector 6 adopts GT101 photodetector, which has high light detection sensitivity, simple structure of the optical density disc, and easy installation. In addition, the power required by the detection circuit is very small, which is harmful to biological The impact of the chip detection system is negligible, and the detection circuit is in a low-power working state, which is safe and reliable.

Claims (5)

1. The biochip detection method with real-time detection of light intensity is characterized in that: an optical density disc is added in the excitation light path of the biochip detection system to adjust the light intensity, and a light detection system is installed under the loading stage to detect The light intensity emitted by the xenon light source and reaching the sample to be tested. This light intensity value truly reflects the real situation of the light intensity of the exciting light source. This light intensity value is recorded in the computer. When the light intensity value changes, the computer The system judges according to the signal provided by the detection circuit, and takes timely measures to adjust the optical density disc or adjust the exposure of the sample to ensure the authenticity and reliability of the detection data. 2. The biochip detection method with real-time detection of light intensity according to claim 1, characterized in that: adjusting the optical density disk or adjusting the exposure of the sample refers to: when the light intensity of the excitation light source changes According to the detected light intensity signal, the computer can automatically control the motor to drive the optical density disk to rotate to the desired position to adjust the incident light intensity of the excitation light. The computer can also change the exposure time setting to adjust the exposure of the sample according to the need. 3. The biochip detection method with real-time detection of light intensity according to claim 1, characterized in that: the optical density disk is made of optical devices that have been coated, and the light transmission and filtration range is between 0° and 0°. In the range of 360°, it is evenly distributed by 99% to 5%. 4. A biochip detection system with real-time detection of light intensity, which includes an optical system, an emission interference filter, a computer, a slide table, a xenon lamp light source, and an excitation interference filter. The optical density disk to adjust the light intensity is installed with a light detector under the slide table. The light emitted by the xenon lamp passes through the optical density disk, and then irradiates the slide table after passing through the excitation interference filter, and the light intensity detector can detect it. After the optical signal is amplified by the integrated operational amplifier, it is sent to the computer for processing, and then the computer controls the adjustment of the optical density disc or the exposure of the sample. 5. The biochip detection system with real-time detection of light intensity according to claim 1, characterized in that: the photodetector is a GT101 photodetector.