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CN1528190A - Oyster multivariate seafood seasoning and preparation method thereof - Google Patents

Oyster multivariate seafood seasoning and preparation method thereof Download PDF

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CN1528190A
CN1528190A CNA2003101048226A CN200310104822A CN1528190A CN 1528190 A CN1528190 A CN 1528190A CN A2003101048226 A CNA2003101048226 A CN A2003101048226A CN 200310104822 A CN200310104822 A CN 200310104822A CN 1528190 A CN1528190 A CN 1528190A
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朱蓓薇
付猛
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Dalian Polytechnic University
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Dalian Institute of Light Industry
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Abstract

一种牡蛎多元海鲜调味料及其制备方法,它是以牡蛎为主要原料,打浆后经常温、低温及紫外线照射自溶后,酶解制成酶解液,再脱除酶解液异味。虾头、扇贝边、小鱼等下脚料加入发酵剂使其发酵,发酵液与水解液混合。将上述混合液过滤澄清后,调pH,再加入食盐、香辛料提取物等辅料调配,浓缩后经造粒烘干制成。本发明的方法能最大限度地保存牡蛎中原有的生理活性物质,添加入下脚料发酵液,即增加了海鲜风味,又减少了牡蛎用量,从而达到了在改善风味的基础上降低成本的目的。本发明的调味料不仅营养丰富,具有独特的海鲜风味,还便于携带和取用,可以用于方便面的调料包等,用途十分广泛。The invention discloses an oyster multi-component seafood seasoning and a preparation method thereof. The oyster is used as a main raw material. After beating, the oyster is autolyzed under constant temperature, low temperature and ultraviolet radiation, and then enzymatically hydrolyzed to form an enzymolyzed liquid, and then the peculiar smell of the enzymatically hydrolyzed liquid is removed. Shrimp heads, scallops, small fish and other leftovers are added with a starter to make it fermented, and the fermented liquid is mixed with the hydrolyzed liquid. After the above mixed solution is filtered and clarified, the pH is adjusted, and auxiliary materials such as salt and spice extracts are added to prepare, concentrated, granulated and dried. The method of the invention can preserve the original physiologically active substances in oysters to the greatest extent, and adding leftovers to fermentation liquid not only increases the flavor of seafood, but also reduces the consumption of oysters, thereby achieving the purpose of reducing costs on the basis of improving the flavor. The seasoning of the invention is not only rich in nutrition, but also has a unique flavor of seafood, is also easy to carry and take, can be used for seasoning packets of instant noodles, etc., and has a wide range of uses.

Description

牡蛎多元海鲜调味料及其制备方法Oyster multivariate seafood seasoning and preparation method thereof

技术领域  本发明涉及一种调味料,特别是海鲜调味料。Technical field The present invention relates to a kind of seasoning, especially seafood seasoning.

背景技术  传统方法生产的蚝油等以牡蛎为主要原料的调味品,产品不精,工艺粗糙,加工的技术水平不够先进,因此不能最大限度地保存牡蛎原有生物活性物质,而且成本高,不易携带。Background Art Oyster sauce and other oyster-based seasonings produced by traditional methods are not refined, the process is rough, and the processing technology is not advanced enough. Therefore, the original biological active substances of oysters cannot be preserved to the maximum extent, and the cost is high and it is not easy to carry.

发明内容  本发明的目的在于提供一种能最大限度地保存牡蛎原有生物活性物质、成本低、易携带的牡蛎多元海鲜调味料。本发明是以牡蛎为主要原料,经适当酶解,将蛋白质水解成即利于吸收,又具有特殊生理功能的生物活性肽,再辅以虾头,扇贝边,小鱼等下脚料的发酵液的方法,以及由该方法获得的产品。SUMMARY OF THE INVENTION The purpose of this invention is to provide an oyster multi-component seafood seasoning that can preserve the original biologically active substances of oysters to the greatest extent, is low in cost and easy to carry. The present invention uses oysters as the main raw material, and through proper enzymolysis, the protein is hydrolyzed into biologically active peptides that are good for absorption and have special physiological functions, and then supplemented with fermentation liquid of leftovers such as shrimp heads, scallops, and small fish. method, and products obtained by the method.

一.原料及处理1. Raw material and processing

1.处理牡蛎1. Processing oysters

取新鲜牡蛎,洗净、剥壳,取出新鲜干净的牡蛎肉,沥干后加1~5倍重的水打浆。Take fresh oysters, wash and shell them, take out the fresh and clean oyster meat, drain and add 1 to 5 times the weight of water for beating.

2.处理下脚料2. Disposal of leftovers

虾头、扇贝边、小鱼分检去杂,漂洗沥干。Shrimp heads, scallop sides, and small fish are sorted to remove impurities, rinse and drain.

二.酶解和发酵2. Enzymolysis and fermentation

(一)酶解:(1) Enzymolysis:

1.自溶后再用外源酶处理1. Autolyzed and then treated with exogenous enzymes

①将上述牡蛎浆放入无菌罐中,在PH6.0~7.5,温度0~4℃自溶24~48小时;①Put the above oyster slurry into a sterile tank, autodissolve at pH 6.0-7.5, temperature 0-4°C for 24-48 hours;

或②将上述牡蛎浆放入无菌罐中,在PH6.0~7.5,常温放置6~8小时;Or ②Put the above oyster slurry into a sterile tank, and place it at a pH of 6.0 to 7.5 at room temperature for 6 to 8 hours;

或③将上述牡蛎浆用紫外线照射10~30分钟,再用浓度为0.06~0.08摩尔/升的氯化钠浸泡,温度为40~65℃(每3分钟升高5℃)的梯度温度,时间30~60分钟。Or ③ irradiate the above-mentioned oyster pulp with ultraviolet light for 10-30 minutes, and then soak it in sodium chloride with a concentration of 0.06-0.08 mol/liter, and the temperature is a gradient temperature of 40-65°C (5°C every 3 minutes). 30-60 minutes.

将采用上述自溶方法之一得到的产物用4~8%柠檬酸调PH至4~5,温度45℃条件下加入0.05~0.4%胃蛋白酶(酶活5×104u/g)酶解1~2小时,之后于90~100℃温度维持20~30分钟灭酶。Adjust the pH of the product obtained by one of the above autolysis methods to 4-5 with 4-8% citric acid, and add 0.05-0.4% pepsin (enzyme activity 5×10 4 u/g) at a temperature of 45°C for enzymatic hydrolysis 1 to 2 hours, and then maintain the temperature at 90 to 100°C for 20 to 30 minutes to inactivate the enzyme.

2.用单外源酶处理2. Treatment with a single exogenous enzyme

将上述牡蛎浆在PH7.0~8.0,加入0.5~2%枯草杆菌中性蛋白酶(酶活4×104u/g),于温度40~55℃酶解1~4小时,之后于90~100℃温度维持20~30分钟灭酶。Add 0.5-2% Bacillus subtilis neutral protease (enzyme activity 4×10 4 u/g) to the above-mentioned oyster slurry at pH 7.0-8.0, enzymolyze it at a temperature of 40-55°C for 1-4 hours, and then decompose it at a temperature of 90-55°C. Keep the temperature at 100°C for 20-30 minutes to inactivate the enzyme.

3.用双外源酶处理3. Treatment with dual exogenous enzymes

①将上述牡蛎浆在PH6.0~8.0,温度40~55℃条件下,加入0.05~0.15%枯草杆菌中性蛋白酶(酶活1.76×105u/g)和0.05~0.25%胰蛋白酶酶(酶活1.86×105u/g)解2~3小时,之后于90~100℃温度维持20~30分钟灭酶。① Add 0.05-0.15% Bacillus subtilis neutral protease (enzyme activity 1.76×10 5 u/g) and 0.05-0.25% trypsinase ( Enzyme activity 1.86×10 5 u/g) Decompose for 2-3 hours, and then maintain the temperature at 90-100°C for 20-30 minutes to inactivate the enzyme.

或②将上述牡蛎浆在PH6.0~8.0,温度45~55℃条件下,加入0.1~0.25%复合蛋白酶(酶活1.11×105u/g)和0.05~0.15%胰蛋白酶酶解(酶活1.86×105u/g)2~3小时,之后于90~100℃温度维持20~30分钟灭酶。Or (2) add 0.1-0.25% compound protease (enzyme activity 1.11×10 5 u/g) and 0.05-0.15% trypsin to hydrolyze the above-mentioned oyster slurry at pH 6.0-8.0 and temperature 45-55°C (enzyme (1.86×10 5 u/g) for 2 to 3 hours, and then maintain the temperature at 90 to 100°C for 20 to 30 minutes to inactivate the enzyme.

(二)发酵(2) Fermentation

1.将上述下脚料置于夹层锅中,蒸煮1~2小时,放入发酵罐中,加入酵母发酵剂1~6%于25~35℃温度发酵2~3个小时。1. Put the leftovers above in a sandwich pot, cook for 1-2 hours, put them into a fermenter, add 1-6% yeast starter, and ferment at 25-35°C for 2-3 hours.

或2.将上述下脚料置于夹层锅中,蒸煮1~2小时,放入发酵罐中,加入红曲发酵剂1~6%于25~35℃温度发酵2~3个小时。Or 2. Put the leftovers above in a jacketed pot, cook for 1-2 hours, put them into a fermenter, add 1-6% of red yeast rice starter, and ferment for 2-3 hours at a temperature of 25-35°C.

或3.将上述下脚料置于夹层锅中,蒸煮1~2小时,放入发酵罐中,加入酵母和红曲复合发酵剂1~6%于25~35℃温度发酵2~3个小时。Or 3. Put the leftovers above in a jacketed pot, cook for 1-2 hours, put them into a fermenter, add 1-6% of yeast and red yeast rice compound starter, and ferment at 25-35°C for 2-3 hours.

三.脱水解液异味3. Odor of dehydrolyzed solution

1.风味酶处理:在PH7.0~8.0,温度在35~45℃条件下,加入0.2~1%复合风味蛋白酶(Flavourzyme)(酶活6.4×104u/g)处理5~15分钟;1. Flavor enzyme treatment: Add 0.2-1% complex flavor protease (Flavourzyme) (enzyme activity 6.4×10 4 u/g) for 5-15 minutes at pH 7.0-8.0 and temperature 35-45°C;

2.糊精和酵母粉处理:水解液中,加入0.8~2%β-环状糊精煮沸2~6分钟,然后加入0.2~0.8%酵母粉于35~45℃加热30分钟;2. Dextrin and yeast powder treatment: add 0.8-2% β-cyclodextrin to the hydrolyzate and boil for 2-6 minutes, then add 0.2-0.8% yeast powder and heat at 35-45°C for 30 minutes;

3.活性炭和糊精处理:水解液中,加入0.1~1%(W/V)活性炭,过滤后加入0.2~1%β-环状糊精煮沸4~8分钟;3. Activated carbon and dextrin treatment: add 0.1-1% (W/V) activated carbon to the hydrolyzate, add 0.2-1% β-cyclodextrin after filtering and boil for 4-8 minutes;

上述方法可单独使用,也可联合使用,脱去水解液异味。The above methods can be used alone or in combination to remove the peculiar smell of the hydrolyzate.

四.澄清4. Clarification

将上述水解液和发酵液混合搅拌均匀,经硅藻土加压过滤或在4000转/分离心10分钟,将得到的澄清液再经孔径为5μm和0.5μm两级微孔滤膜过滤,彻底清除其中细小悬浮颗粒和细菌。Mix and stir the above-mentioned hydrolyzed liquid and fermented liquid evenly, pressurize and filter through diatomaceous earth or centrifuge at 4000 rpm for 10 minutes, and then filter the obtained clarified liquid through two-stage microporous membranes with a pore size of 5 μm and 0.5 μm, thoroughly Remove fine suspended particles and bacteria.

五.调配Five. Deployment

在上述滤液中,加入食盐6~12%,糖4~8%,味精2~4%,洋葱粉1~3%,姜蒜粉1~3%,胡椒粉0.5~2%,小茴香精油1~3%,搅拌均匀。In the above filtrate, add 6-12% of salt, 4-8% of sugar, 2-4% of monosodium glutamate, 1-3% of onion powder, 1-3% of ginger and garlic powder, 0.5-2% of pepper powder, cumin essential oil 1 ~3%, stir evenly.

六.后处理6. Post-processing

1.浓缩:将调配好的液体置于真空浓缩设备中,控制真空度86.25~98.64Kpa,温度45~65℃浓缩,使固形物在12~15%。1. Concentration: Put the prepared liquid in a vacuum concentration equipment, control the vacuum degree to 86.25-98.64Kpa, and concentrate at a temperature of 45-65°C, so that the solid content is 12-15%.

2.喷雾造粒:放入喷雾造粒机,制成粒度在0.5~1mm,水分在4%以下的颗粒。2. Spray granulation: Put it into a spray granulator to make granules with a particle size of 0.5-1mm and a moisture content of less than 4%.

3.包装3. Packaging

在上述工艺中,在调PH值时,酸用4~8%的柠檬酸,碱用5~10%的氢氧化钠。In the above process, when adjusting the pH value, 4-8% citric acid is used for the acid, and 5-10% sodium hydroxide is used for the alkali.

本发明与现有技术相比具有如下优点:Compared with the prior art, the present invention has the following advantages:

1.本发明能够最大限度地保存牡蛎中原有的生理活性物质,而且利用现代生物技术将蛋白质水解成生物活性肽,使产品兼有营养性和功能性。1. The present invention can preserve the original physiologically active substances in oysters to the greatest extent, and utilize modern biotechnology to hydrolyze proteins into biologically active peptides, so that the product has both nutrition and functionality.

2.本发明以牡蛎为主要原料,以虾头、扇贝边、小鱼等下脚料为辅料,既降低了成本,又增强了海鲜风味。2. The present invention uses oysters as the main raw material, and uses leftovers such as shrimp heads, scallops, and small fish as auxiliary materials, which not only reduces the cost, but also enhances the seafood flavor.

3.本发明中的脱腥处理不仅效果好,不存在无机盐的异味,而且还可将腥味物质转化为呈鲜香味的化合物3. The deodorization treatment in the present invention is not only effective, and there is no peculiar smell of inorganic salts, but also can convert fishy substances into compounds with fresh fragrance

4.本发明的调味料海鲜风味浓郁,独特,保质期长,携带方便,用途十分广泛。4. The seasoning seafood flavor of the present invention is rich and unique, has a long shelf life, is easy to carry, and has a wide range of uses.

5.通过生物工程技术生产海鲜调味料,不但深度转化了海洋生物资源而且产品形式非常迎合人们对海鲜特殊营养、特殊风味调味品的日益增长的需求趋势。生产对海产下脚料的转化,减轻了沿海地区的环境污染,其经济效益、环境效益和社会效益显著。5. The production of seafood seasoning through bioengineering technology not only deeply transforms marine biological resources, but also caters to people's growing demand for seafood with special nutrition and special flavor seasoning. The transformation of seafood leftovers in production reduces environmental pollution in coastal areas, and its economic, environmental and social benefits are remarkable.

具体实施方式Detailed ways

例1example 1

取新鲜牡蛎洗净、剥壳,取牡蛎肉50公斤,加入250公斤水,用打浆机打浆。将牡蛎浆放入无菌罐中,在PH为6,温度为4℃的条件下,放置24小时。用8%柠檬酸将PH调至4,加热使温度在45℃,加入200克(0.4%)胃蛋白酶(酶活5×104U/g)酶解1小时。之后用蒸汽加热至90℃,维持30分钟灭酶。然后用10%氢氧化钠将PH调至8,加入250g复合风味酶(酶活6.4×104U/g)于45℃处理5分钟。Wash and shell fresh oysters, take 50 kg of oyster meat, add 250 kg of water, and beat with a beater. Put the oyster slurry into a sterile tank, and place it for 24 hours at a pH of 6 and a temperature of 4°C. The pH was adjusted to 4 with 8% citric acid, heated to 45°C, and 200 g (0.4%) pepsin (enzyme activity 5×10 4 U/g) was added for enzymatic hydrolysis for 1 hour. Then heat to 90°C with steam and maintain for 30 minutes to inactivate the enzyme. Then adjust the pH to 8 with 10% sodium hydroxide, add 250 g of compound flavor enzyme (enzyme activity: 6.4× 10 4 U/g) and treat at 45° C. for 5 minutes.

取新鲜虾头、扇贝边、小鱼,除杂,漂洗,取100公斤,在夹层锅内蒸煮1小时,之后放入发酵罐中,加入酵母发酵剂1公斤,在30℃下发酵3小时。之后发酵液和水解液放入混合机内混合均匀,经离心机在4000转/分,离心10分钟,取上清液。然后再经过孔径为5μm和0.5μm两级微孔滤膜过滤。将滤液用4%的柠檬酸调节PH值为6.7,加入食盐40公斤(10%),糖16公斤(4%),味精12公斤(4%),洋葱粉9公斤(3%),姜蒜粉7.5公斤(2.5%),胡椒粉4.5公斤(1.5%),小茴香精油3公斤(1.0%),经混合机搅拌均匀。之后置于真空度86.25Kpa的真空浓缩设备中,于65℃温度浓缩,当浓缩液固形物为12%时,再将其移入喷雾造粒机中喷雾造粒。筛分后装袋,即可得到牡蛎多元海鲜调味料。其各种成分百分含量如下:Take fresh shrimp heads, scallops, and small fish, remove impurities, rinse, take 100 kg, cook in a sandwich pot for 1 hour, then put it into a fermentation tank, add 1 kg of yeast starter, and ferment at 30 ° C for 3 hours. Afterwards, the fermented liquid and the hydrolyzed liquid were put into a mixer and mixed evenly, centrifuged at 4000 rpm for 10 minutes by a centrifuge, and the supernatant was taken. Then it is filtered through two-stage microporous membranes with a pore size of 5 μm and 0.5 μm. The filtrate is adjusted with 4% citric acid to be 6.7, add 40 kg (10%) of salt, 16 kg (4%) of sugar, 12 kg (4%) of monosodium glutamate, 9 kg (3%) of onion powder, ginger and garlic 7.5 kilograms (2.5%) of ground powder, 4.5 kilograms (1.5%) of pepper powder, 3 kilograms (1.0%) of cumin essential oil, stir through mixer. Then place it in a vacuum concentrating device with a vacuum degree of 86.25Kpa, concentrate at a temperature of 65° C., and when the solid content of the concentrated solution is 12%, move it into a spray granulator for spray granulation. After sieving and bagging, the oyster multivariate seafood seasoning can be obtained. Its various composition percentages are as follows:

蛋白质                       31.8Protein 31.8

多肽混合物                   16.3Peptide mixture 16.3

水分                         4Moisture 4

还原糖                       5Reducing sugar 5

总氨基酸                     1.5Total Amino Acids 1.5

游离氨基酸                   0.75Free Amino Acids 0.75

牛磺酸                       0.30Taurine 0.30

例2Example 2

取新鲜牡蛎洗净、剥壳,取牡蛎肉50公斤,加入200公斤水,用打浆机打浆。将牡蛎浆放入无菌罐中,在PH为7.5,温度为0℃的条件下,放置12小时。用8%柠檬酸将PH调至5,加热使温度在45℃,加入25克(0.05%)胃蛋白酶(酶活5×104U/g)酶解2小时。之后用蒸汽加热至100℃,维持20分钟灭酶。然后用5%氢氧化钠将PH调至7,加入200克复合风味酶(酶活6.4×104U/g)于35℃处理15分钟。Wash and shell fresh oysters, take 50 kg of oyster meat, add 200 kg of water, and beat with a beater. Put the oyster slurry into a sterile tank, and place it for 12 hours at a pH of 7.5 and a temperature of 0°C. The pH was adjusted to 5 with 8% citric acid, heated to 45°C, and 25 g (0.05%) of pepsin (enzyme activity 5×10 4 U/g) was added for enzymatic hydrolysis for 2 hours. Then heat to 100°C with steam for 20 minutes to inactivate the enzyme. Then adjust the pH to 7 with 5% sodium hydroxide, add 200 grams of compound flavor enzyme (enzyme activity: 6.4× 10 4 U/g) and treat at 35° C. for 15 minutes.

取新鲜虾头、扇贝边、小鱼,除杂,漂洗,取100公斤,在夹层锅内蒸煮2小时,之后放入发酵罐中,加入红曲发酵剂3公斤,在35℃下发酵2小时。之后发酵液和水解液放入混合机内混合均匀,经离心机在4000转/分,离心10分钟,取上清液。然后再经过孔径为5μm和0.5μm两级微孔滤膜过滤。将滤液用4%的柠檬酸调节PH值为6.6,加入食盐42公斤(12%),糖17.5公斤(5%),味精10.5公斤(3%),洋葱粉7公斤(2%),姜蒜粉3.5公斤(1%),胡椒粉1.75公斤(0.5%),小茴香精油7公斤(2.0%),下面重复例1的后处理操作,得到牡蛎多元海鲜调味料。其各种成分百分含量如下:Take fresh shrimp heads, scallops, and small fish, remove impurities, rinse, take 100 kg, cook in a sandwich pot for 2 hours, then put them in a fermentation tank, add 3 kg of red yeast yeast, and ferment for 2 hours at 35°C . Afterwards, the fermented liquid and the hydrolyzed liquid were put into a mixer and mixed evenly, centrifuged at 4000 rpm for 10 minutes by a centrifuge, and the supernatant was taken. Then it is filtered through two-stage microporous membranes with a pore size of 5 μm and 0.5 μm. The filtrate is adjusted with 4% citric acid to be 6.6, add 42 kg (12%) of salt, 17.5 kg (5%) of sugar, 10.5 kg (3%) of monosodium glutamate, 7 kg (2%) of onion powder, ginger and garlic 3.5 kilograms (1%) of powder, 1.75 kilograms (0.5%) of pepper powder, 7 kilograms (2.0%) of fennel essential oil, repeat the post-processing operation of example 1 below, obtain oyster multivariate seafood seasoning. Its various composition percentages are as follows:

蛋白质                            30.8protein 30.8

多肽混合物                         16.6Peptide mixture 16.6

水分                               3.9Moisture 3.9

还原糖                             5.8Reducing sugar 5.8

总氨基酸                           1.36Total Amino Acids 1.36

游离氨基酸                         0.72Free Amino Acids 0.72

牛磺酸                             0.48Taurine 0.48

例3Example 3

取新鲜牡蛎洗净、剥壳,取牡蛎肉50公斤,加入100公斤水用打浆机打浆。将牡蛎浆放入无菌罐中,在PH为6,温度为22℃的条件下,放置8小时。用8%柠檬酸将PH调至4,加热使温度在45℃,加入50克胃蛋白酶(酶活5×104U/g)酶解1.5小时。之后用蒸汽加热至90℃,维持30分钟灭酶。然后用5%氢氧化钠将PH调至8,加入500g复合风味酶(酶活6.4×104U/g)于35℃处理10分钟。再加0.4公斤β-环状糊精煮沸6分钟,然后加入400g酵母粉于45℃加热30分钟。Wash and shell fresh oysters, take 50 kg of oyster meat, add 100 kg of water and beat with a beater. Put the oyster slurry into a sterile tank, and place it for 8 hours under the condition that the pH is 6 and the temperature is 22°C. The pH was adjusted to 4 with 8% citric acid, heated to 45°C, and 50 g of pepsin (enzyme activity 5×10 4 U/g) was added for enzymatic hydrolysis for 1.5 hours. Then heat to 90°C with steam and maintain for 30 minutes to inactivate the enzyme. Then adjust the pH to 8 with 5% sodium hydroxide, add 500 g of compound flavor enzyme (enzyme activity: 6.4× 10 4 U/g) and treat at 35° C. for 10 minutes. Add another 0.4 kg of β-cyclodextrin and boil for 6 minutes, then add 400 g of yeast powder and heat at 45° C. for 30 minutes.

取新鲜虾头、扇贝边、小鱼,除杂,漂洗,取100公斤,在夹层锅内蒸煮2小时,之后放入发酵罐中,加入复合发酵剂5公斤,在30℃下发酵2小时。之后发酵液和水解液放入混合机内混合均匀,经离心机在4000转/分,离心10分钟,取上清液。然后再经过孔径为5μm和0.5μm两级微孔滤膜过滤。将滤液用4%的柠檬酸调节PH值为6.8,加入食盐22.5公斤(9%),糖20公斤(8%),味精7.5公斤(3%),洋葱粉7.5公斤(3%),姜蒜粉5公斤(2.%),胡椒粉1.25公斤(0.5%),小茴香精油2.5公斤(1.0%),下面重复例1的后处理操作,得到牡蛎多元海鲜调味料。其各种成分百分含量如下:Take fresh shrimp heads, scallops, and small fish, remove impurities, rinse, take 100 kg, cook in a sandwich pot for 2 hours, then put them into a fermentation tank, add 5 kg of compound starter, and ferment at 30°C for 2 hours. Afterwards, the fermented liquid and the hydrolyzed liquid were put into a mixer and mixed evenly, centrifuged at 4000 rpm for 10 minutes by a centrifuge, and the supernatant was taken. Then it is filtered through two-stage microporous membranes with a pore size of 5 μm and 0.5 μm. The filtrate is adjusted with 4% citric acid to be 6.8, add 22.5 kg (9%) of salt, 20 kg (8%) of sugar, 7.5 kg (3%) of monosodium glutamate, 7.5 kg (3%) of onion powder, ginger and garlic 5 kilograms (2.%) of powder, 1.25 kilograms (0.5%) of pepper powder, 2.5 kilograms (1.0%) of cumin essential oil, repeat the post-processing operation of example 1 below, obtain the multivariate seafood seasoning of oyster. Its various composition percentages are as follows:

蛋白质                             32.1Protein 32.1

多肽混合物                         15.7Peptide mixture 15.7

水分                               3.6Moisture 3.6

还原糖                          5.9Reducing sugar 5.9

总氨基酸                        1.4Total Amino Acids 1.4

游离氨基酸                      0.7Free amino acid 0.7

牛磺酸                          0.6Taurine 0.6

例4Example 4

取新鲜牡蛎洗净、剥壳,取牡蛎肉50公斤,加入50公斤水用打浆机打浆。将牡蛎浆放入无菌发酵罐中,用氢氧化钠调PH为7.0,加入500克(1%)枯草杆菌中性蛋白酶(酶活4×104u/g),于温度45℃酶解3小时,之后于100℃温度维持20分钟灭酶。加入1公斤(1%)活性炭,过滤后加250克(0.5%)β-环状糊精煮沸8分钟。Wash and shell fresh oysters, take 50 kg of oyster meat, add 50 kg of water, and beat with a beater. Put the oyster pulp into a sterile fermenter, adjust the pH to 7.0 with sodium hydroxide, add 500 grams (1%) of Bacillus subtilis neutral protease (enzyme activity 4×10 4 u/g), and enzymolyze at 45°C 3 hours, and then maintain at 100°C for 20 minutes to inactivate the enzyme. Add 1 kg (1%) of activated carbon, filter and add 250 g (0.5%) of β-cyclodextrin and boil for 8 minutes.

取新鲜虾头、扇贝边、小鱼,除杂,漂洗,取100公斤,在夹层锅内蒸煮1小时,之后放入发酵罐中,加入红曲发酵剂4公斤,在35℃下发酵2小时。之后发酵液和水解液放入混合机内混合均匀,经离心机在4000转/分,离心10分钟,取上清液。然后再经过孔径为5μm和0.5μm两级微孔滤膜过滤。将滤液用4%的柠檬酸调节PH值为6.8,加入食盐12公斤(6%),糖10公斤(5%),味精4公斤(2%),洋葱粉2公斤(1%),姜蒜粉3公斤(1.5%),胡椒粉公斤(0.15%),小茴香精油2公斤(1%),下面重复例1的后处理操作,得到牡蛎多元海鲜调味料。其各种成分百分含量如下:Take fresh shrimp heads, scallops, and small fish, remove impurities, rinse, take 100 kg, cook in a sandwich pot for 1 hour, then put it into a fermentation tank, add 4 kg of red yeast yeast, and ferment for 2 hours at 35°C . Afterwards, the fermented liquid and the hydrolyzed liquid were put into a mixer and mixed evenly, centrifuged at 4000 rpm for 10 minutes by a centrifuge, and the supernatant was taken. Then it is filtered through two-stage microporous membranes with a pore size of 5 μm and 0.5 μm. The filtrate is adjusted to a pH value of 6.8 with 4% citric acid, 12 kg (6%) of salt, 10 kg (5%) of sugar, 4 kg of monosodium glutamate (2%), 2 kg of onion powder (1%), ginger and garlic are added. 3 kilograms (1.5%) of powder, 1 kilogram of pepper powder (0.15%), 2 kilograms (1%) of cumin essential oil, repeat the post-processing operation of example 1 below, obtain the multivariate seafood seasoning of oyster. Its various composition percentages are as follows:

蛋白质                          30.0Protein 30.0

多肽混合物                      15.4Peptide mixture 15.4

水分                            3.5Moisture 3.5

还原糖                          5.9Reducing sugar 5.9

总氨基酸                        1.21Total Amino Acids 1.21

游离氨基酸                      0.73Free Amino Acids 0.73

牛磺酸                          0.31Taurine 0.31

例5Example 5

取新鲜牡蛎洗净、剥壳,取牡蛎肉50公斤,加入150公斤水,用打浆机打浆。将牡蛎浆放入无菌罐中,用10%氢氧化钠调节PH为7.5,在温度为50℃的条件下,加入50克(0.1%)枯草杆菌中性蛋白酶(酶活1.76×105U/g)和100g(0.2%)胰蛋白酶(酶活1.86×105U/g),酶解2小时,之后于95℃温度维持20分钟灭酶。再加400gβ-环状糊精煮沸6分钟之后,加入400g酵母粉于45℃加热30分钟。Wash and shell fresh oysters, take 50 kg of oyster meat, add 150 kg of water, and beat with a beater. Put the oyster slurry into a sterile tank, adjust the pH to 7.5 with 10% sodium hydroxide, and add 50 grams (0.1%) of Bacillus subtilisin neutral protease (enzyme activity 1.76×10 5 U) at a temperature of 50°C /g) and 100g (0.2%) trypsin (enzyme activity 1.86×10 5 U/g), hydrolyze for 2 hours, and then maintain the temperature at 95°C for 20 minutes to inactivate the enzyme. After adding 400g of β-cyclodextrin and boiling for 6 minutes, add 400g of yeast powder and heat at 45°C for 30 minutes.

取新鲜虾头、扇贝边、小鱼,除杂,漂洗,取100公斤,在夹层锅内蒸煮1小时,之后放入发酵罐中,加入复合发酵剂4公斤,在35℃下发酵2小时。之后发酵液和水解液放入混合机内混合均匀,经离心机在4000转/分,离心10分钟,取上清液。然后再经过孔径为5μm和0.5μm两级微孔滤膜过滤。将滤液用4%的柠檬酸调节PH值为6.8,加入食盐30公斤(10%),糖18公斤(6%),味精9公斤(3%),洋葱粉6公斤(2%),姜蒜粉6公斤(2%),胡椒粉4.5公斤(1.5%),小茴香精油6公斤(2%),下面重复例1的后处理操作,得到牡蛎多元海鲜调味料。其各种成分百分含量如下:Take fresh shrimp heads, scallops, and small fish, remove impurities, rinse, take 100 kg, cook in a sandwich pot for 1 hour, then put them into a fermentation tank, add 4 kg of compound starter, and ferment at 35°C for 2 hours. Afterwards, the fermented liquid and the hydrolyzed liquid were put into a mixer and mixed evenly, centrifuged at 4000 rpm for 10 minutes by a centrifuge, and the supernatant was taken. Then it is filtered through two-stage microporous membranes with a pore size of 5 μm and 0.5 μm. The filtrate is adjusted with 4% citric acid to be 6.8, add 30 kg (10%) of salt, 18 kg (6%) of sugar, 9 kg of monosodium glutamate (3%), 6 kg of onion powder (2%), ginger and garlic 6 kilograms (2%) of powder, 4.5 kilograms (1.5%) of pepper powder, 6 kilograms (2%) of cumin essential oil, repeat the post-processing operation of example 1 below, obtain oyster multivariate seafood seasoning. Its various composition percentages are as follows:

蛋白质                             33.6Protein 33.6

多肽混合物                         15.8Peptide mixture 15.8

水分                               3.6Moisture 3.6

还原糖                             5.6Reducing sugar 5.6

总氨基酸                           1.5Total Amino Acids 1.5

游离氨基酸                         0.8Free Amino Acids 0.8

牛磺酸                             0.75Taurine 0.75

例6Example 6

取新鲜牡蛎洗净、剥壳,取牡蛎肉50公斤,加入50公斤水,用打浆机打浆。将牡蛎浆放入无菌罐中,用10%氢氧化钠调节PH为7.0,在温度55℃条件下,加入50克(0.1%)复合蛋白酶(酶活1.11×105u/g)和75克(0.15%)胰蛋白酶酶解(酶活1.86×105u/g)3小时,之后于90℃温度维持30分钟灭酶。加入0.4公斤β-环状糊精煮沸5分钟,然后加入400g酵母粉于45℃加热30分钟。Wash and shell fresh oysters, take 50 kg of oyster meat, add 50 kg of water, and beat with a beater. Put the oyster slurry into a sterile tank, adjust the pH to 7.0 with 10% sodium hydroxide, and add 50 grams (0.1%) of compound protease (enzyme activity 1.11×10 5 u/g) and 75 gram (0.15%) of trypsin was hydrolyzed (enzyme activity 1.86×10 5 u/g) for 3 hours, and then kept at 90°C for 30 minutes to inactivate the enzyme. Add 0.4 kg of β-cyclodextrin and boil for 5 minutes, then add 400 g of yeast powder and heat at 45° C. for 30 minutes.

取新鲜虾头、扇贝边、小鱼,除杂,漂洗,取100公斤,在夹层锅内蒸煮2小时,之后放入发酵罐中,加入复合发酵剂4公斤,在35℃下发酵3小时。之后发酵液和水解液放入混合机内混合均匀,经离心机在4000转/分,离心10分钟,取上清液。然后再经过孔径为5μm和0.5μm两级微孔滤膜过滤。将滤液用4%的柠檬酸调节PH值为6.8,加入食盐12公斤(6%),糖12公斤(6%),味精4公  2%),洋葱粉2公斤(1%),姜蒜粉5公斤(2.5%),胡椒粉6公斤(2%),小茴香精油4公斤(2%),下面重复例1的后处理操作,得到牡蛎多元海鲜调味料。其各种成分百分含量如下:Take fresh shrimp heads, scallops, and small fish, remove impurities, rinse, take 100 kg, cook in a sandwich pot for 2 hours, then put them into a fermenter, add 4 kg of compound starter, and ferment at 35°C for 3 hours. Afterwards, the fermented liquid and the hydrolyzed liquid were put into a mixer and mixed evenly, centrifuged at 4000 rpm for 10 minutes by a centrifuge, and the supernatant was taken. Then it is filtered through two-stage microporous membranes with a pore size of 5 μm and 0.5 μm. The filtrate is adjusted with 4% citric acid to be 6.8, add 12 kg (6%) of salt, 12 kg (6%) of sugar, 4 kg (2%) of monosodium glutamate, 2 kg (1%) of onion powder, ginger and garlic powder 5 kilograms (2.5%), 6 kilograms (2%) of pepper powder, 4 kilograms (2%) of cumin essential oil, repeat the post-processing operation of example 1 below, obtain oyster multivariate seafood seasoning. Its various composition percentages are as follows:

蛋白质                             30.1Protein 30.1

多肽混合物                         15.6Peptide mixture 15.6

水分                               3.6Moisture 3.6

还原糖                             4.8Reducing sugar 4.8

总氨基酸                           1.70Total Amino Acids 1.70

游离氨基酸                         0.42Free Amino Acids 0.42

牛磺酸                             0.26Taurine 0.26

注:本申请文件所涉及的比例均为重量比。Note: The proportions involved in this application document are weight ratios.

Claims (10)

1. the polynary seafood condiment of a seed oyster, it is characterized in that: it contains following component in percentage by weight:
Protein 〉=30%
Mixtures of polypeptides 〉=15%
Moisture≤4%
Reduced sugar 2~5%
Total amino acid 0.4~1.5%
Free amino acid 0.25~0.75%
Taurine 0.25~1.0%
2. the polynary seafood condiment of above-mentioned oyster is characterized in that:
(1) preparation of enzymolysis liquid
1. raw material and processing: get clean bright oyster meat, add the water making beating of 1~5 times of weight after draining;
2. enzymolysis:
A starches above-mentioned oyster in PH6.0~7.5,0~4 ℃ of self-dissolving of temperature 24~48 hours;
B adds 0.05~0.4% pepsin in 45 ℃ of following enzymolysis 1~2 hour at PH4~5;
C is 90~100 ℃ in temperature to be kept 20~30 minutes.
3. take off the hydrolyzate peculiar smell
(2) preparation of fermentation liquid
1. raw material and processing: with rinsings such as shrimp head, scallop edge, fish offal material, drain;
2. fermentation:
After the above-mentioned raw materials pulverizing, boiling 1~2 hour adds 1~6% leavening in 25~35 ℃ of fermentations 2~3 hours;
(3) clarification
Said hydrolyzed liquid and zymotic fluid are mixed, through diatomite pressure filtration or 4000 rev/mins centrifugal 10 minutes, with the clarified solution that obtains again via hole diameter be 5 μ m and 0.5 μ m two-stage filtering with microporous membrane;
(4) allotment
In above-mentioned filtrate, add salt 6~12%, sugar 4~8%, monosodium glutamate 2~4%, onion powder 1~3%, ginger garlic powder 1~3%, pepper powder 0.5~2%, Fructus Foeniculi quintessence oil 1~3% stirs.
(5) concentrate, granulation, oven dry
With above-mentioned deployed liquid concentration, make graininess through comminutor, packing is finished product.
3. the polynary seafood condiment of above-mentioned oyster is characterized in that:
(1) preparation of enzymolysis liquid
1. raw material and processing: get clean bright oyster meat, add the water making beating of 1~5 times of weight after draining;
2. enzymolysis:
In PH6.0~7.5, placed 6~8 hours by normal temperature with above-mentioned oyster slurry for a;
B adds 0.05~0.4% pepsin in 45 ℃ of following enzymolysis 1~2 hour at PH4~5;
C is 90~100 ℃ in temperature to be kept 20~30 minutes.
3. take off the hydrolyzate peculiar smell
(2) preparation of fermentation liquid
1. raw material and processing: with rinsings such as shrimp head, scallop edge, fish offal material, drain;
2. fermentation:
After the above-mentioned raw materials pulverizing, boiling 1~2 hour adds 1~6% leavening in 25~35 ℃ of fermentations 2~3 hours;
(3) clarification
Said hydrolyzed liquid and zymotic fluid are mixed, through diatomite pressure filtration or 4000 rev/mins centrifugal 10 minutes, with the clarified solution that obtains again via hole diameter be 5 μ m and 0.5 μ m two-stage filtering with microporous membrane;
(4) allotment
In above-mentioned filtrate, add salt 6~12%, sugar 4~8%, monosodium glutamate 2~4%, onion powder 1~3%, ginger garlic powder 1~3%, pepper powder 0.5~2%, Fructus Foeniculi quintessence oil 1~3% stirs.
(5) concentrate, granulation, oven dry
With above-mentioned deployed liquid concentration, make graininess through comminutor, packing is finished product.
4. the polynary seafood condiment of above-mentioned oyster is characterized in that:
(1) preparation of enzymolysis liquid
1. raw material and processing: get clean bright oyster meat, add the water making beating of 1~5 times of weight after draining;
2. enzymolysis:
A with ultraviolet ray irradiation 10~30 minutes, is the sodium chloride immersion of 0.06~0.08 mol with above-mentioned oyster slurry with concentration again, and temperature is the gradient temperature of 40~65 ℃ (raising 5 ℃ in per 3 minutes), 30~60 minutes time;
B adds 0.05~0.4% pepsin in 45 ℃ of following enzymolysis 1~2 hour at PH4~5;
C is 90~100 ℃ in temperature to be kept 20~30 minutes.
3. take off the hydrolyzate peculiar smell
(2) preparation of fermentation liquid
1. raw material and processing: with rinsings such as shrimp head, scallop edge, fish offal material, drain;
2. fermentation:
After the above-mentioned raw materials pulverizing, boiling 1~2 hour adds 1~6% leavening in 25~35 ℃ of fermentations 2~3 hours;
(3) clarification
Said hydrolyzed liquid and zymotic fluid are mixed, through diatomite pressure filtration or 4000 rev/mins centrifugal 10 minutes, with the clarified solution that obtains again via hole diameter be 5 μ m and 0.5 μ m two-stage filtering with microporous membrane;
(4) allotment
In above-mentioned filtrate, add salt 6~12%, sugar 4~8%, monosodium glutamate 2~4%, onion powder 1~3%, ginger garlic powder 1~3%, pepper powder 0.5~2%, Fructus Foeniculi quintessence oil 1~3% stirs.
(5) concentrate, granulation, oven dry
With above-mentioned deployed liquid concentration, make graininess through comminutor, packing is finished product.
5. the polynary seafood condiment of above-mentioned oyster is characterized in that:
(1) preparation of enzymolysis liquid
1. raw material and processing: get clean bright oyster meat, add the water making beating of 1~5 times of weight after draining;
2. enzymolysis: above-mentioned oyster slurry in PH7.0~8.0, is added 0.5~2% bacillus subtilis neutral proteinase, in 40~55 ℃ of enzymolysis of temperature 1~4 hour.
3. take off the hydrolyzate peculiar smell
(2) preparation of fermentation liquid
1. raw material and processing: with rinsings such as shrimp head, scallop edge, fish offal material, drain;
2. fermentation:
After the above-mentioned raw materials pulverizing, boiling 1~2 hour adds 1~6% leavening in 25~35 ℃ of fermentations 2~3 hours;
(3) clarification
Said hydrolyzed liquid and zymotic fluid are mixed, through diatomite pressure filtration or 4000 rev/mins centrifugal 10 minutes, with the clarified solution that obtains again via hole diameter be 5 μ m and 0.5 μ m two-stage filtering with microporous membrane;
(4) allotment
In above-mentioned filtrate, add salt 6~12%, sugar 4~8%, monosodium glutamate 2~4%, onion powder 1~3%, ginger garlic powder 1~3%, pepper powder 0.5~2%, Fructus Foeniculi quintessence oil 1~3% stirs.
(5) concentrate, granulation, oven dry
With above-mentioned deployed liquid concentration, make graininess through comminutor, packing is finished product.
6. the polynary seafood condiment of a seed oyster is characterized in that:
(1) preparation of enzymolysis liquid
1. raw material and processing: get clean bright oyster meat, add the water making beating of 1~5 times of weight after draining;
2. enzymolysis: above-mentioned oyster slurry in PH6.0~8.0, under 40~55 ℃ of conditions of temperature, was added 0.05~0.15% bacillus subtilis neutral proteinase and 0.05~0.25% trypsin digestion 2~3 hours
3. take off the hydrolyzate peculiar smell
(2) preparation of fermentation liquid
1. raw material and processing: with rinsings such as shrimp head, scallop edge, fish offal material, drain;
2. fermentation:
After the above-mentioned raw materials pulverizing, boiling 1~2 hour adds 1~6% leavening in 25~35 ℃ of fermentations 2~3 hours;
(3) clarification
Said hydrolyzed liquid and zymotic fluid are mixed, through diatomite pressure filtration or 4000 rev/mins centrifugal 10 minutes, with the clarified solution that obtains again via hole diameter be 5 μ m and 0.5 μ m two-stage filtering with microporous membrane;
(4) allotment
In above-mentioned filtrate, add salt 6~12%, sugar 4~8%, monosodium glutamate 2~4%, onion powder 1~3%, ginger garlic powder 1~3%, pepper powder 0.5~2%, Fructus Foeniculi quintessence oil 1~3% stirs.
(5) concentrate, granulation, oven dry
With above-mentioned deployed liquid concentration, make graininess through comminutor, packing is finished product.
7. the polynary seafood condiment of above-mentioned oyster is characterized in that:
(1) preparation of enzymolysis liquid
1. raw material and processing: get clean bright oyster meat, add the water making beating of 1~5 times of weight after draining;
2. enzymolysis: above-mentioned oyster slurry in PH6.0~8.0, under 45~55 ℃ of conditions of temperature, was added 0.1~0.25% compound protease and 0.05~0.15% trypsin digestion 2~3 hours
3. take off the hydrolyzate peculiar smell
(2) preparation of fermentation liquid
1. raw material and processing: with rinsings such as shrimp head, scallop edge, fish offal material, drain;
2. fermentation:
After the above-mentioned raw materials pulverizing, boiling 1~2 hour adds 1~6% leavening in 25~35 ℃ of fermentations 2~3 hours;
(3) clarification
Said hydrolyzed liquid and zymotic fluid are mixed, through diatomite pressure filtration or 4000 rev/mins centrifugal 10 minutes, with the clarified solution that obtains again via hole diameter be 5 μ m and 0.5 μ m two-stage filtering with microporous membrane;
(4) allotment
In above-mentioned filtrate, add salt 6~12%, sugar 4~8%, monosodium glutamate 2~4%, onion powder 1~3%, ginger garlic powder 1~3%, pepper powder 0.5~2%, Fructus Foeniculi quintessence oil 1~3% stirs.
(5) concentrate, granulation, oven dry
With above-mentioned deployed liquid concentration, make graininess through comminutor, packing is finished product.
8. according to claim 2 or 3 or 4 or 5 or the 6 or 7 polynary seafood condiments of described oyster, it is characterized in that: the peculiar smell that removes the oyster enzymolysis liquid with food flavor enzyme, be in PH7.0~8.0, temperature added 0.2~1% composite flavor Protease Treatment 5~15 minutes under 35~45 ℃ of conditions.
9. according to claim 2 or 3 or 4 or 5 or the 6 or 7 polynary seafood condiments of described oyster, it is characterized in that: the peculiar smell that removes the oyster enzymolysis liquid with dextrin and yeast, be in hydrolyzate, add 0.8~2% cycloheptaamylose and boiled 2~6 minutes, add 0.2~0.8% dusty yeast then in 35~45 ℃ of heating 30 minutes.
10. according to claim 2 or 3 or 4 or 5 or the 6 or 7 polynary seafood condiments of described oyster, it is characterized in that: the peculiar smell that removes the oyster enzymolysis liquid with active carbon and dextrin, be in hydrolyzate, add 0.1~1% (W/V) active carbon, filter back adding 0.2~1% cycloheptaamylose and boiled 4~8 minutes.
CNA2003101048226A 2003-10-10 2003-10-10 Oyster multivariate seafood seasoning and preparation method thereof Pending CN1528190A (en)

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CN101268824B (en) * 2008-05-09 2011-02-16 李新民 Method for preparing sea sausage seasoning matter
CN102028172A (en) * 2010-12-18 2011-04-27 北海市源龙珍珠有限公司 Method for preparing flavoring from taurine-extracted pearl oyster meat
CN102084978A (en) * 2009-12-08 2011-06-08 泰宝美客株式会社 Novel seafood seasoning and the manufacturing method thereof
CN102132855A (en) * 2011-03-22 2011-07-27 尹渭元 Ocean fish protein peptide composite seasoning powder and preparation method thereof
CN101536764B (en) * 2009-02-25 2012-05-30 广东海洋大学 Preparation method of oyster delicate flavor seasoning
CN102524510A (en) * 2010-12-14 2012-07-04 大连工业大学 Preparation method of low-fluorine Euphasia superb protein base stock
CN102972735A (en) * 2012-11-30 2013-03-20 烟台泰华海洋食品股份有限公司 Fan shell adductor seafood sauce and processing method thereof
CN103099180A (en) * 2013-01-09 2013-05-15 烟台泰华海洋食品股份有限公司 Short necked clam seafood condiment sauce and preparation method thereof
CN103892238A (en) * 2014-04-15 2014-07-02 海南大学 Seasoning material product prepared by applying cooked juice of mussels and preparation method of seasoning material product
CN103976338A (en) * 2014-05-29 2014-08-13 赵祥忠 Method for producing low-sodium seafood compound condiment
CN104187556A (en) * 2014-08-05 2014-12-10 北海富安源生物科技有限公司 Method for producing natural seasoning from oysters
CN104886603A (en) * 2015-06-17 2015-09-09 青岛海百合生物技术有限公司 Seafood seasoning rich in GABA (gamma-aminobutyric acid) and preparation method of seafood seasoning
CN105192665A (en) * 2015-10-19 2015-12-30 海南大学 Shrimp-flavored liquid as well as preparation method and application thereof
CN105475950A (en) * 2015-12-13 2016-04-13 宋介珍 Preparation method for nutritional seasoner for making soup stock
CN105614814A (en) * 2015-12-22 2016-06-01 崔为山 Seafood condiment and making technology thereof
CN105876722A (en) * 2016-04-26 2016-08-24 韦白玲 Seafood flavored seasoning
CN106107635A (en) * 2016-06-29 2016-11-16 大连深蓝肽科技研发有限公司 Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide
CN107865374A (en) * 2016-09-26 2018-04-03 威海红印食品科技有限公司 A kind of compound seafood seasoned food
CN108935719A (en) * 2018-08-31 2018-12-07 广东环境保护工程职业学院 Shellfish enzymolysis liquid beans custard and preparation method thereof
CN109452607A (en) * 2018-11-07 2019-03-12 北京康宝利华生物科技有限公司 Baste and preparation method thereof comprising abalone enzymatic extract
CN114376197A (en) * 2022-01-20 2022-04-22 中国海洋大学 Freshness enhancing agent and preparation method thereof
CN117297062A (en) * 2023-09-06 2023-12-29 青岛海洋食品营养与健康创新研究院 Euphausia superba composite seafood oyster sauce and preparation method thereof

Cited By (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101268824B (en) * 2008-05-09 2011-02-16 李新民 Method for preparing sea sausage seasoning matter
CN101536764B (en) * 2009-02-25 2012-05-30 广东海洋大学 Preparation method of oyster delicate flavor seasoning
CN102084978B (en) * 2009-12-08 2014-03-12 泰宝美客株式会社 Novel seafood seasoning and manufacturing method thereof
CN102084978A (en) * 2009-12-08 2011-06-08 泰宝美客株式会社 Novel seafood seasoning and the manufacturing method thereof
CN102524510A (en) * 2010-12-14 2012-07-04 大连工业大学 Preparation method of low-fluorine Euphasia superb protein base stock
CN102028172A (en) * 2010-12-18 2011-04-27 北海市源龙珍珠有限公司 Method for preparing flavoring from taurine-extracted pearl oyster meat
CN102132855A (en) * 2011-03-22 2011-07-27 尹渭元 Ocean fish protein peptide composite seasoning powder and preparation method thereof
CN102972735A (en) * 2012-11-30 2013-03-20 烟台泰华海洋食品股份有限公司 Fan shell adductor seafood sauce and processing method thereof
CN103099180A (en) * 2013-01-09 2013-05-15 烟台泰华海洋食品股份有限公司 Short necked clam seafood condiment sauce and preparation method thereof
CN103892238A (en) * 2014-04-15 2014-07-02 海南大学 Seasoning material product prepared by applying cooked juice of mussels and preparation method of seasoning material product
CN103976338A (en) * 2014-05-29 2014-08-13 赵祥忠 Method for producing low-sodium seafood compound condiment
CN103976338B (en) * 2014-05-29 2016-02-10 齐鲁工业大学 A kind of production method of low sodium type seafood compound seasoner
CN104187556A (en) * 2014-08-05 2014-12-10 北海富安源生物科技有限公司 Method for producing natural seasoning from oysters
CN104187556B (en) * 2014-08-05 2016-05-11 北海富安源生物科技有限公司 A kind of method of producing natural condiment containing taking oyster as raw material
CN104886603A (en) * 2015-06-17 2015-09-09 青岛海百合生物技术有限公司 Seafood seasoning rich in GABA (gamma-aminobutyric acid) and preparation method of seafood seasoning
CN105192665A (en) * 2015-10-19 2015-12-30 海南大学 Shrimp-flavored liquid as well as preparation method and application thereof
CN105192665B (en) * 2015-10-19 2018-04-06 海南大学 One seed shrimp liquid flavor and preparation method and application
CN105475950A (en) * 2015-12-13 2016-04-13 宋介珍 Preparation method for nutritional seasoner for making soup stock
CN105614814A (en) * 2015-12-22 2016-06-01 崔为山 Seafood condiment and making technology thereof
CN105876722A (en) * 2016-04-26 2016-08-24 韦白玲 Seafood flavored seasoning
CN106107635A (en) * 2016-06-29 2016-11-16 大连深蓝肽科技研发有限公司 Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide
CN107865374A (en) * 2016-09-26 2018-04-03 威海红印食品科技有限公司 A kind of compound seafood seasoned food
CN108935719A (en) * 2018-08-31 2018-12-07 广东环境保护工程职业学院 Shellfish enzymolysis liquid beans custard and preparation method thereof
CN109452607A (en) * 2018-11-07 2019-03-12 北京康宝利华生物科技有限公司 Baste and preparation method thereof comprising abalone enzymatic extract
CN109452607B (en) * 2018-11-07 2021-11-23 武汉华腾济康生物科技有限公司 Sauce containing enzymatic extract of abalone and its preparation method
CN114376197A (en) * 2022-01-20 2022-04-22 中国海洋大学 Freshness enhancing agent and preparation method thereof
CN117297062A (en) * 2023-09-06 2023-12-29 青岛海洋食品营养与健康创新研究院 Euphausia superba composite seafood oyster sauce and preparation method thereof

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