CN106615686A - Feed additive containing shrimp processing by-product namely protein powder, as well as preparation method and application of feed additive - Google Patents

Abstract

The invention discloses a feed additive containing shrimp processing by-product protein powder, which consists of the following components in parts by weight: 5-10 parts of shrimp processing by-product protein powder, 0.5-1 part of rosemary extract, 1-3 parts Parts of carvacrol, 30-40 parts of feed grade zeolite powder. The feed additive containing shrimp processing by-product protein powder of the present invention has simple components, good preservation and dispersibility, wide application range and low cost, and is very suitable for popularization and application in feed. The invention also discloses a method for preparing a feed additive containing the by-product protein powder of shrimp processing, which comprises the following steps: (1) preparing the protein powder of the by-product of shrimp processing; (2) compounding. The preparation method of the present invention has simple process steps, stable and controllable process, energy saving, high efficiency, and environmental protection, can reduce environmental pollution by shrimp processing by-products, and provide a practical basis for enterprises to realize resource utilization of shrimp processing by-products, which is beneficial Further improve the economic value of shrimp.

Description

A feed additive containing shrimp processing by-product protein powder and its preparation method, application

technical field

The invention relates to a feed additive, in particular to a feed additive containing shrimp processing by-product protein powder and its preparation method and application.

Background technique

At the same time, my country's feed industry is developing rapidly, and feed resources, especially protein resources, are in short supply. The development of new protein feeds has become a top priority. At present, the protein sources in the feed are mainly various fish meal and shrimp meal, etc., which are costly and are added in an extensive way, that is, the fish meal and shrimp are directly crushed and mixed into the feed, which is not conducive to the digestion and absorption of animals. Low, especially for all kinds of cubs and seedlings, whose digestive system is immature and their digestion ability is weak, and eating a large amount may even cause diarrhea and other phenomena.

my country is rich in shrimp production, and the processed and exported products mainly include prawns, freshwater crayfish shrimp soup, etc. In recent years, with the increase of aquatic products enterprises, more and more by-products such as shrimp heads and shrimp shells are produced during the processing. The by-products of shrimp processing contain a lot of nutrients such as protein, but most of them are discarded, causing serious environmental pollution and waste of resources.

If the by-products of shrimp processing are processed into protein powder, and it is compounded into an additive for supplementing protein and added to the feed to increase the protein content, it can not only reduce the environmental pollution of the by-products of shrimp processing, but also to a certain extent On the one hand, it can meet the animal's demand for protein in the breeding process and reduce the feed cost, which can be said to kill two birds with one stone.

For example, the application publication number CN105815549A, the Chinese patent of application publication date 2016.08.03 discloses an amino acid high protein powder feed additive and a preparation method thereof. The amino acid high protein powder feed additive is mainly composed of the following raw materials in parts by weight: amino acid high protein powder 30～ 50; traditional Chinese medicine additives 5-10; methionine 1-5; vitamin B12 1-5; calcium formate 10-30; galactomannan 5-15; L-carnitine 15-20; yeast nucleotides 10-20; Soybean lecithin 15-25; acidulant 5-8. The feed additive has high cost, poor preservation and dispersibility, and is difficult to uniformly disperse in the feed.

Contents of the invention

The present invention aims to solve the above-mentioned technical problems in the prior art, and provides a feed additive containing shrimp processing by-product protein powder with simple components, good preservation and dispersibility, wide application range and low cost.

The invention also provides a preparation method of a feed additive containing shrimp processing by-product protein powder, which has simple process steps, stable and controllable process, energy saving, high efficiency, environmental protection, and good product safety and stability.

In order to achieve the above object, the present invention adopts the following technical solutions:

A feed additive containing shrimp processing by-product protein powder, which consists of the following components in parts by weight: 5-10 parts of shrimp processing by-product protein powder, 0.5-1 part of rosemary extract, and 1-3 parts of carvacrol , 30-40 feed grade zeolite powder. The present invention designs a feed additive containing shrimp processing by-product protein powder suitable for various feeds according to the characteristics that the protein powder of the by-product of shrimp processing is easy to deteriorate and is not easy to disperse. The present invention uses the protein powder of the by-product of shrimp processing as protein supplement Compared with protein sources such as fish meal and shrimp meal, the cost is lower. Feed grade zeolite powder is used as a premix and carrier, which can fully disperse the protein powder of the by-product of shrimp processing, which solves the problem that the protein powder of the by-product of shrimp processing is not easy to disperse. At the same time, feed-grade zeolite powder also contains a variety of trace elements, which can make up for the lack of trace elements in the feed, and can absorb and inhibit the reproduction of various bacteria, thereby delaying the deterioration of the by-product protein powder of shrimp processing to a certain extent; parsley Phenol has a strong function of inhibiting and killing a variety of pathogenic microorganisms. Rosemary powder has strong antioxidant and antibacterial properties. Carvacrol and rosemary powder are synergistic and can effectively inhibit shrimp Process by-product protein powder spoilage, browning and other phenomena, improve its shelf life. The feed additive containing shrimp processing by-product protein powder of the present invention has simple components, good preservation and dispersibility, wide application range and low cost, and is very suitable for popularization and application in feed.

A method for preparing a feed additive containing shrimp processing by-product protein powder, comprising the following steps:

(1) Preparation of shrimp processing by-product protein powder

(1) After washing the by-products of shrimp processing, drying them, adding heat-conducting metal powders for dry grinding and pulverizing to obtain raw material powders. The shrimp processing by-products in the present invention are mainly shrimp heads and shrimp shells, and protein powder is prepared by using the shrimp processing by-products as the main raw material, which can reduce the environmental pollution of the shrimp processing by-products and realize resource utilization of the shrimp processing by-products for enterprises It is beneficial to further improve the economic value of shrimp by providing a practical basis; when the by-products of shrimp processing are dry-grinded, a large amount of heat will be generated, which will cause the protein in the by-products of shrimp processing to be denatured by heat, and it is easy to make the by-products of shrimp processing agglomerate , not easy to crush, in order to solve the above problems, the present invention creatively adds heat-conducting metal powder into the by-products of shrimp processing during dry grinding and crushing. The most important thing about the agglomeration of by-products is that it has excellent thermal conductivity, which can quickly dissipate the heat generated during grinding and crushing, reduce the thermal denaturation of protein in shrimp processing by-products, and improve the crushing quality and protein extraction rate.

(2) Place the raw material powder in ethanol for 30-60 minutes of ultrasonic treatment, filter and dry. Sonication in ethanol was used to remove the lipids in the raw material powder, which was beneficial for the next step of enzymatic hydrolysis.

(3) Add the raw material powder in step (2) into hydrochloric acid with a mass fraction of 4-6%, soak for 30-60 min, filter, wash with water until neutral, and dry. Soak the raw material powder in hydrochloric acid to remove the calcium carbonate in the raw material powder and form interconnected channels in the raw material powder. These channels are conducive to the entry of enzymes to increase the contact area and greatly increase the rate of enzymatic hydrolysis. , shortening the enzymatic hydrolysis time (enzymolysis 2h is enough), is conducive to improving the efficiency of protein extraction.

(4) Add the raw material powder in step (3) into water, adjust the material-liquid ratio to 1:10-20, and obtain the raw material liquid; when the temperature is raised to 50-55°C, add papain, and perform constant temperature enzymatic hydrolysis for 1-2 hours, adjust After the pH is 7.5-8, add alkaline protease, perform constant temperature enzymatic hydrolysis for 0.5-1 hour, raise the temperature to inactivate the enzyme, and obtain the enzymatic hydrolysis solution. The enzymatic hydrolysis solution of shrimp processing by-products will have a bitter taste, and the resulting product has poor palatability. The bitter taste produced in the enzymatic protein is mainly related to the hydrophobic amino acids in the protein. The higher the content of hydrophobic amino acids in the protein, the greater the bitterness. In the present invention, papain and alkaline protease are selected for step-by-step enzymatic hydrolysis, and the enzymatic hydrolysis effect is good, and the bitter taste of the product is found to be significantly reduced. The reason may be that papain and alkaline protease synergistically reduce the content of hydrophobic amino acids in the protein.

(5) After centrifuging and filtering the enzymolysis solution, the supernatant solution is vacuum-concentrated and vacuum-freeze-dried to obtain protein powder, a by-product of shrimp processing, for use. Vacuum freeze-drying, the product has no browning and good color

(2) Compounding

After weighing each component according to the above weight ratio, first mix the rosemary extract and carvacrol evenly, then add protein powder, a by-product of shrimp processing, and mix evenly, and finally add feed-grade zeolite powder, and sieve, that is A feed additive containing protein powder, a by-product of shrimp processing, is obtained. Step-by-step mixing is carried out, the process steps are simple, and the operability is strong.

Preferably, in step (1), the mass ratio of the thermally conductive metal powder to the shrimp processing by-product is 1:5-7, and the thermally conductive metal powder is copper powder or stainless steel powder.

Preferably, in step (1), the particle size of the raw material powder is controlled at 300-500 mesh.

As preferably, in step (4), based on the mass of the raw material liquid, the amount of papain added is 5000-6000 U/g.

As a preference, in step (4), based on the mass of the raw material liquid, the amount of alkaline citrullin added is 2000-5000 U/g.

As a preference, in step (4), the specific process step of raising the temperature to kill the enzyme is: raising the temperature to 95-100° C. and keeping it for 10-20 minutes.

Preferably, in step (4), the enzymolysis process is carried out under stirring conditions.

Preferably, in step (5), the vacuum concentration is completed when the solid content is 30-40%.

The application of a feed additive containing shrimp processing by-product protein powder in feed.

Therefore, the present invention has following beneficial effect:

(1) Provide a feed additive containing shrimp processing by-product protein powder, using shrimp processing by-product protein powder as a protein supplement, with simple components, good preservation and dispersibility, wide application range, low cost, very suitable for Promotion and application in feed;

(2) When dry grinding and crushing the by-products of shrimp processing, adding heat-conducting metal powder to the by-products of shrimp processing not only facilitates the crushing of by-products of shrimp processing, but also avoids the agglomeration of by-products of shrimp processing during the grinding and crushing process, reducing the The protein in the processing by-products is denatured by heat, which can improve the crushing quality and protein extraction rate;

(3) Papain and alkaline protease are selected for step-by-step enzymatic hydrolysis, which can significantly reduce the bitterness of the product.

detailed description

The present invention will be further described below through specific embodiments.

Example 1

(1) Preparation of shrimp processing by-product protein powder

(1) After washing the by-products of shrimp processing, dry them, and add heat-conducting metal powders for dry grinding according to the mass ratio of heat-conducting metal powder (copper powder) and shrimp processing by-products in a ratio of 1:7 to obtain a particle size of 500 mesh raw material powder;

(2) Place raw material powder in ethanol for 60 minutes of ultrasonic treatment, filter, and dry;

(3) adding the raw material powder in step (2) into hydrochloric acid with a mass fraction of 4-6%, soaking for 60 minutes, filtering, washing with water until neutral, and drying;

(4) Add the raw material powder in step (3) into water, adjust the material-liquid ratio to 1:20, and obtain the raw material liquid; when the temperature is raised to 55°C, add papain under stirring conditions, and the mass of the raw material liquid is papaya The amount of protease added is 6000U/g, constant temperature enzymatic hydrolysis for 2h, after adjusting the pH to 8, add alkaline protease, based on the mass of the raw material liquid, the amount of alkaline guarinase added is 5000U/g, constant temperature enzymatic hydrolysis for 1h, and the temperature is raised to 100°C Keep for 20 minutes to inactivate the enzyme to obtain the enzymatic hydrolysis solution;

(5) After centrifuging and filtering the enzymatic solution, vacuum concentrate the supernatant to a solid content of 40%, and vacuum freeze-dry to obtain protein powder, a by-product of shrimp processing, for use;

(2) Compounding

According to the weight ratio of 10kg shrimp processing by-product protein powder, 1kg rosemary extract, 3kg carvacrol, and 40kg feed grade zeolite powder, mix the rosemary extract and carvacrol evenly Finally, add shrimp processing by-product protein powder and mix evenly, and finally add feed-grade zeolite powder and sieve to obtain a feed additive containing shrimp processing by-product protein powder.

Example 2

(1) Preparation of shrimp processing by-product protein powder

(1) After cleaning the by-products of shrimp processing, dry them, and add heat-conducting metal powders for dry grinding according to the mass ratio of heat-conducting metal powder (copper powder) and shrimp processing by-products in a ratio of 1:6 to obtain a particle size of 400 mesh raw material powder;

(2) Place the raw material powder in ethanol for ultrasonic treatment for 40 minutes, filter, and dry;

(3) adding the raw material powder in the step (2) into hydrochloric acid with a mass fraction of 5% and soaking for 50 min, filtering, washing with water until neutral, and drying;

(4) Add the raw material powder in step (3) into water, and adjust the ratio of material to liquid to 1:15 to obtain the raw material liquid; when the temperature is raised to 52°C, add papain under stirring conditions, and the mass of the raw material liquid is papaya The addition of protease is 5500U/g, constant temperature enzymolysis for 1.2h, after adjusting the pH to 7.8, add alkaline protease, based on the mass of raw material liquid, the addition of alkaline guarinase is 3000U/g, constant temperature enzymolysis for 0.7h, and heat up to Keep at 98°C for 15 minutes to inactivate the enzyme, and obtain the enzymatic hydrolysis solution;

(5) After centrifuging and filtering the enzymatic solution, vacuum concentrate the supernatant to a solid content of 35%, and vacuum freeze-dry to obtain protein powder, a by-product of shrimp processing, for use;

(2) Compounding

After weighing each component according to the weight ratio of 8kg shrimp processing by-product protein powder, 0.7kg rosemary extract, 2kg carvacrol, and 35kg feed grade zeolite powder, firstly mix rosemary extract and carvacrol After uniformity, add shrimp processing by-product protein powder and mix evenly, and finally add feed-grade zeolite powder and sieve to obtain a feed additive containing shrimp processing by-product protein powder.

Example 3

(1) Preparation of shrimp processing by-product protein powder

(1) After washing the by-products of shrimp processing, dry them, and add heat-conducting metal powders for dry grinding according to the mass ratio of heat-conducting metal powder (copper powder) and shrimp processing by-products in a ratio of 1:7 to obtain a particle size of 500 mesh raw material powder;

(2) Place raw material powder in ethanol for 60 minutes of ultrasonic treatment, filter, and dry;

(3) adding the raw material powder in step (2) to 6% hydrochloric acid for soaking for 60 min, filtering, washing with water until neutral, and drying;

(4) Add the raw material powder in step (3) into water, adjust the material-liquid ratio to 1:20, and obtain the raw material liquid; when the temperature is raised to 55°C, add papain under stirring conditions, and the mass of the raw material liquid is papaya The amount of protease added is 6000U/g, constant temperature enzymatic hydrolysis for 2h, after adjusting the pH to 8, add alkaline protease, based on the mass of the raw material liquid, the amount of alkaline guarinase added is 5000U/g, constant temperature enzymatic hydrolysis for 1h, and the temperature is raised to 100°C Keep for 20 minutes to inactivate the enzyme to obtain the enzymatic hydrolysis solution;

(5) After centrifuging and filtering the enzymatic solution, vacuum concentrate the supernatant to a solid content of 40%, and vacuum freeze-dry to obtain protein powder, a by-product of shrimp processing, for use;

(2) Compounding

According to the weight ratio of 10kg shrimp processing by-product protein powder, 1kg rosemary extract, 3kg carvacrol, and 40kg feed grade zeolite powder, mix the rosemary extract and carvacrol evenly Finally, add shrimp processing by-product protein powder and mix evenly, and finally add feed-grade zeolite powder and sieve to obtain a feed additive containing shrimp processing by-product protein powder.

The feed additive containing shrimp processing by-product protein powder of the present invention can be added to any feed as a protein supplement, and has a wide range of applications, and the actual addition amount can be adjusted according to animal needs.

The embodiment described above is only a preferred solution of the present invention, and does not limit the present invention in any form. There are other variations and modifications on the premise of not exceeding the technical solution described in the claims.

Claims (10)

1. a kind of feed addictive containing shrimp processing byproduct albumen powder, it is characterised in that by the component group of following weight portion Into：5～10 parts of shrimp processing byproduct albumen powders, 0.5～1 part of Rosmarinus officinalis extract, 1～3 part of carvacrol, 30～40 parts of feed grades Zeolite powder.

2. a kind of feed addictive preparation method containing shrimp processing byproduct albumen powder as claimed in claim 1, its feature It is to comprise the following steps：

(1) shrimp processing byproduct albumen powder is prepared

(1) after shrimp processing byproduct is cleaned, drying adds heat-conducting metal powder to carry out dry grinding crushing, obtains raw material powder；

(2) raw material powder is placed in into ultrasonically treated 30～60min in ethanol, is filtered, drying；

(3) the raw material powder in step (2) is added in the hydrochloric acid that mass fraction is 4～6% and soaks 30～60min, filtered, use water Neutrality is washed till, is dried；

(4) the raw material powder in step (3) is added to the water, it is 1 to adjust solid-liquid ratio：10～20, obtain material liquid；It is warming up to 50～55 DEG C when, add papain, constant temperature digest 1～2h, adjust pH be 7.5～8 after, add alkali protease, constant temperature enzymolysis 0.5 ～1h, heat up the enzyme that goes out, and obtains enzymolysis liquid；

(5) after by enzymolysis liquid centrifugal filtration, clear liquid is carried out into concentrated in vacuo, vacuum freeze drying, obtains final product shrimp processing byproduct egg White powder, it is stand-by；

(2) compound

Weigh after each component by above-mentioned weight, after first Rosmarinus officinalis extract is well mixed with carvacrol, add shrimp Processing byproduct albumen powder is well mixed, and is eventually adding feed grade zeolite powder, sieves, and obtains final product containing shrimp processing byproduct albumen powder Feed addictive.

3. the feed addictive preparation method containing shrimp processing byproduct albumen powder according to claim 2, its feature exists In, in step (1), the mass ratio 1 of the heat-conducting metal powder and shrimp processing byproduct：5～7, heat-conducting metal powder is copper powder Or stainless steel powder.

4. the feed addictive preparation method containing shrimp processing byproduct albumen powder according to claim 2, its feature exists In in step (1), the size controlling of raw material powder is in 300～500 mesh.

5. the feed addictive preparation method containing shrimp processing byproduct albumen powder according to claim 2, its feature exists In, in step (4), in terms of material liquid quality, the 5000～6000U/g of addition of papain.

6. the feed addictive preparation method containing shrimp processing byproduct albumen powder according to claim 2, its feature exists In, in step (4), in terms of material liquid quality, the 2000～5000U/g of addition of alkaline melon protease.

7. the feed addictive preparation method containing shrimp processing byproduct albumen powder according to claim 2, its feature exists In in step (4), the go out concrete technology step of enzyme of intensification is：It is warming up to 95～100 DEG C of 10～20min of holding.

8. the feed addictive preparation method containing shrimp processing byproduct albumen powder according to claim 2, its feature exists In in step (4), enzymolysis process is carried out under agitation.

9. the method that a kind of utilization shrimp processing byproduct according to claim 2 prepares albumen powder, it is characterised in that step (5) in, it is concentrated in vacuo to when solid content is 30～40% and terminates concentration.

10. a kind of application of the feed addictive containing shrimp processing byproduct albumen powder in feed as claimed in claim 1.