CN118294685A - High-speed processor for pretreatment of tubular mass spectrum sample - Google Patents

Abstract

The present application relates to the technical field of biomedical equipment, and in particular to a high-speed processor for pre-processing of tubular mass spectrometry samples, which includes a box body; a reagent compartment for placing reagent tubes; a sample compartment for placing sample tubes; a reaction cup holder for placing reaction cups; a gun tip compartment for placing gun tips; a waste box for placing used reaction cups and gun tips; a mixing module including a support frame, an oscillation mechanism and a liquid collection device, the oscillation mechanism is used to mix reagents and samples, and the liquid collection device is used to absorb supernatant; a first track; a transfer trolley slidably connected to the first track; a first mechanical arm for grabbing the reaction cup and placing the reaction cup on the transfer trolley; a second mechanical arm for absorbing reagents and injecting the reagents into the reaction cups on the transfer trolley; a third mechanical arm for absorbing samples and injecting the samples into the reaction cups on the transfer trolley. The present application has the effect of shortening the time of sample pre-processing to improve the efficiency of sample pre-processing.

Description

A high-speed processor for pre-treatment of tubular mass spectrometry samples

Technical Field

The present application relates to the field of biomedical equipment technology, and in particular to a high-speed processor for pre-processing of tubular mass spectrometry samples.

Background technique

Mass spectrometry is an ion charge-to-mass ratio analysis method that can be used to analyze isotopic composition, organic structure, and elemental composition. Mass spectrometry can provide rich structural information in one analysis. Combining separation technology with mass spectrometry is a breakthrough in separation science. Mass spectrometry technology is developing rapidly. With the development of mass spectrometry technology, the application field of mass spectrometry technology is becoming wider and wider. Since mass spectrometry analysis has the advantages of high sensitivity, small sample amount, fast analysis speed, and simultaneous separation and identification, mass spectrometry technology is widely used in chemistry, chemical engineering, environment, energy, medicine, sports medicine, criminal investigation science, life science, material science and other fields.

At present, when performing mass spectrometry analysis on samples, from sampling to writing the analysis report, it can be roughly divided into four steps: sample collection, sample pretreatment, analysis and detection, and data processing and reporting results. In the entire analysis process, sample pretreatment accounts for about 70% of the entire analysis process, or even more.

Since the matrix of biological samples is complex and the targets to be detected are mostly endogenous compounds, such as serum samples containing proteins, peptides, amino acids, lipids, sugars, inorganic salts, vitamins, organic acids, hormones, etc., in order to reduce matrix interference, extend the service life of chromatographic columns, prevent system blockage and damage, and improve detection sensitivity and specificity, it is often necessary to perform pre-treatment such as precipitation, extraction, purification, and enrichment on the samples. At present, pre-treatment instruments are usually used to pre-treat samples.

The pre-treatment instrument described in the related art mainly includes a mixing unit and a robotic arm. The robotic arm is used to grab the reaction cup, absorb samples and reagents. When performing sample pre-treatment, the robotic arm absorbs samples and reagents according to the corresponding process and injects them into the reaction cup. The mixing unit homogenizes the mixed liquid in the reaction cup, and then precipitates, extracts, purifies, and enriches to obtain the liquid to be tested. In this way, in order to achieve the purpose of multiple liquid aspiration and liquid injection, the robotic arm needs to move over a large range multiple times, which makes the efficiency of sample pre-treatment low and the sample pre-treatment time long.

Summary of the invention

In order to shorten the sample pre-processing time and improve the sample pre-processing efficiency, the present application provides a high-speed processor for tubular mass spectrometry sample pre-processing.

The present application provides a high-speed processor for pre-processing of tubular mass spectrometer samples using the following technical solution:

A high-speed processor for pre-processing of tubular mass spectrometry samples, comprising a box, a reagent compartment, a sample compartment, a reaction cup holder, a gun head compartment, a waste box, a mixing module, a first mechanical arm, a second mechanical arm, a third mechanical arm, a first track and a transfer trolley;

The reagent compartment is installed in the box body and is used to place reagent tubes containing reagents;

The sample compartment is installed in the box body and is used to place a sample tube containing a sample;

The reaction cup holder is installed in the box and is used to place the reaction cup;

The gun tip bin is installed in the box and is used to place gun tips for aspiration. The third mechanical arm absorbs the gun tips before aspiration.

The waste box is installed in the box body and is used to store used reaction cups and gun tips;

The mixing module is installed in the box, and includes a support frame, an oscillating mechanism installed on the support frame, and a liquid collecting device installed on the support frame, wherein the oscillating mechanism is used to mix the reagent and the sample evenly, and the liquid collecting device is used to absorb the supernatant in the reaction cup;

The first robotic arm, the second robotic arm, the third robotic arm, the first track and the transfer cart are all arranged in the box body, the transfer cart is slidably connected to the first track, and the transfer cart can be moved to the bottom of the first robotic arm, the second robotic arm and the third robotic arm respectively, the first robotic arm is used to grab the reaction cup and place the reaction cup on the transfer cart, the second robotic arm is used to absorb the reagent and inject the reagent into the reaction cup on the transfer cart, and the third robotic arm is used to absorb the sample and inject the sample into the reaction cup on the transfer cart.

By adopting the above technical solution, when pre-processing the sample, the transfer cart moves to the bottom of the first robotic arm, the first robotic arm grabs the reaction cup from the reaction cup holder and places the reaction cup on the transfer cart, and then the transfer cart moves to the bottom of the second robotic arm, the second robotic arm sucks each reagent in turn according to the processing requirements and adds it to the reaction cup of the transfer cart, and then the transfer cart moves to the bottom of the third robotic arm, the third robotic arm sucks the gun tip from the gun tip warehouse, and then sucks the sample and adds it to the reaction cup of the transfer cart, the third robotic arm returns and withdraws the used gun tip above the waste box, and at the same time the transfer cart moves to the bottom of the first robotic arm again, the first robotic arm grabs the reaction cup and places the reaction cup in the mixing module for vibration, and finally completes the sample pre-processing work. In this way, through the cooperation of the first robotic arm, the second robotic arm, the third robotic arm and the transfer cart, the first robotic arm, the second robotic arm and the third robotic arm only need to complete the tasks of taking the cup, adding reagents and adding samples respectively, and each robotic arm no longer needs to move over a large range, which greatly shortens the time for taking the cup, aspirating liquid and injecting liquid, thereby shortening the time for sample pretreatment and improving the efficiency of sample pretreatment.

Optionally, it also includes a support base installed on the box body and a guide rail installed on the support base, the guide rail is arranged along the length direction of the box body, and the first robotic arm, the second robotic arm, and the third robotic arm are all slidably connected to the guide rail.

By adopting the above technical solution, the three robotic arms move on the same guide rail, which ensures the accuracy of the X-axis movement, and also facilitates the installation and positioning of the three robotic arms, thereby reducing the installation cost.

Optionally, three first driving mechanisms are provided on the support seat, and the three first driving mechanisms are arranged one by one corresponding to the first robotic arm, the second robotic arm, and the third robotic arm, and are used to drive the first robotic arm, the second robotic arm, and the third robotic arm to move along the setting direction of the guide rail.

By adopting the above technical solution, the three robotic arms are driven by three separate driving mechanisms, which ensures the independence of the three robotic arms when working, prevents them from interfering with each other, facilitates collaborative work, and further improves work efficiency.

Optionally, it also includes a second track and a transfer trolley slidably connected to the second track, the transfer trolley is equipped with a swing arm, the first track and the second track are parallel, the swing arm can grab the reaction cup on the transfer trolley, and the transfer trolley is used to deliver the reaction cup to the detection instrument.

By adopting the above technical solution, after the sample is pre-processed, it is placed on the transfer cart, the transfer cart moves to the side of the transfer cart, the swing arm grabs the reaction cup on the transfer cart, and then the transfer cart moves to the side of the detection instrument, and the detection instrument can detect the target object in the reaction cup.

Optionally, the reaction cup placed in the mixing module contains magnetic beads capable of adsorbing the target substance, and the mixing module further includes a magnetic attraction mechanism installed on the support frame for fixing the position of the magnetic beads in the reaction cup.

By adopting the above technical solution, when the sample and the magnetic bead solution are fully mixed, the target object to be detected can be adsorbed on the magnetic beads. During the cleaning process, the magnetic attraction mechanism can produce an adsorption effect on the magnetic beads, thereby reducing the residual non-target substances on the magnetic beads, increasing the concentration of the target substance, and improving the detection accuracy.

Optionally, the magnetic attraction mechanism includes a mounting seat, a magnet disposed on the mounting seat, and a lifting assembly disposed on the mounting seat, and the lifting assembly is used to drive the magnet to rise or descend along the axial direction of the reaction cup.

By adopting the above technical solution, when oscillating, the lifting component drives the magnet down, the magnetic beads are no longer affected by the magnetic attraction, and the cleaning liquid can fully clean the magnetic beads. After the oscillation is over, the lifting component drives the magnet up, the magnetic beads are fixed, and the liquid collection device takes away the supernatant liquid to complete the purification treatment of the target object.

Optionally, a liquid preparation pool and a cleaning pool are further provided on the support frame, the liquid collection device includes a liquid collection needle, the liquid preparation pool is used to prepare the solution required in the sample purification process for the liquid collection needle to absorb, and the cleaning pool is used to clean the liquid collection needle.

By adopting the above technical solution, the liquid preparation tank, cleaning tank and mixing module are integrated together to improve the processing efficiency.

Optionally, a driving motor is installed on the reagent chamber, and a main gear is fixedly connected to the output shaft of the driving motor. The main gear is located at the bottom of the reagent chamber, and the main gear is meshed with a slave gear. The slave gear can be sleeved and fixed on the outer wall of the reagent tube. When the reagent tube is inserted from the reagent chamber, the main gear and the slave gear can be meshed.

By adopting the above technical solution, when the reagent tube is inserted from the top of the reagent compartment, the main gear and the slave gear mesh and rotate, thereby driving the reagent tube to shake, thereby ensuring the homogenization of the reagent.

Optionally, a cleaning unit is provided on the reagent chamber for cleaning the pipette needle on the second robotic arm.

By adopting the above technical solution, after the aspiration needle on the second robot arm absorbs the reagent, the cleaning unit can directly clean the aspiration needle on the second robot arm. In this way, the second robot arm does not need to move over a large range, and at the same time, the space in the reagent compartment is fully utilized to achieve space rationalization.

Optionally, a cup sorter is also included, and the cup sorter is used to sort the reaction cups and transport each reaction cup to the reaction cup holder.

By adopting the above technical solution, the cup sorter arranges the reaction cups as required, and the first robotic arm can directly grab and use them without manual placement, which improves convenience during use.

In summary, the present application includes at least one of the following beneficial technical effects:

In the present application, a first mechanical arm, a second mechanical arm, a third mechanical arm, a first track and a transfer trolley are provided. The transfer trolley slides on the first track, the first mechanical arm is used to grab the reaction cup, the second mechanical arm is used to absorb the reagent, and the third mechanical arm is used to absorb the sample. When performing sample pre-treatment, the transfer trolley moves to the bottom of the first mechanical arm, the first mechanical arm grabs the reaction cup and places it on the transfer trolley, and then the transfer trolley moves with the reaction cup to the bottom of the second mechanical arm, at which time the second mechanical arm absorbs the reagent and injects it into the reaction cup, and then the transfer trolley continues to move with the reaction cup to the bottom of the third mechanical arm, the third mechanical arm absorbs the sample and injects it into the reaction cup, and finally, the transfer trolley moves to the bottom of the first mechanical arm, and the first mechanical arm grabs the reaction cup and moves it to the corresponding station for subsequent processing. In this way, through the cooperation of the first robotic arm, the second robotic arm, the third robotic arm and the transfer trolley, each robotic arm only needs to complete its own work, and can achieve synchronous cooperation, and no longer need the robotic arm to move over a large range, greatly shortening the time for aspiration and injection, thereby shortening the time for sample pretreatment and improving the efficiency of sample pretreatment;

The present application also provides a cup sorter, which can sort the reaction cups, so that manual placement is no longer required. Multiple reaction cups are placed in the cup sorter, and the cup sorter sorts the reaction cups as required, and the first robotic arm can directly grab and use them, which improves the convenience of use.

The present application also includes a mixing mechanism, which mainly includes an oscillation component and a magnetic suction component. The oscillation component can drive the reaction cup to oscillate so that the sample and the reagent are fully mixed, and the magnetic beads fully absorb the substance to be detected. After sufficient oscillation and mixing, the magnetic suction component absorbs the magnetic beads on the side wall of the reaction cup, and the aspiration needle absorbs the supernatant in the reaction cup. After repeating several times, the enrichment of the target substance is achieved, thereby improving the accuracy of the detection results.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a schematic diagram of the structure of a high-speed processor for pre-processing of tubular mass spectrometry samples in the present application;

FIG2 is a top view of a high-speed processor for pre-processing of tubular mass spectrometry samples in the present application;

FIG3 is a schematic diagram of the structure of the sample chamber and the reagent chamber in the present application;

FIG4 is a top view of the sample compartment and the reagent compartment in the present application after the top shell is hidden;

FIG5 is a schematic diagram of the structure of the reagent strip in the present application;

FIG6 is a schematic structural diagram of the first mechanical arm in the present application;

FIG7 is a schematic diagram of the installation of the transfer trolley and the transfer trolley in the present application;

FIG8 is a schematic diagram of the structure of the mixing module in the present application;

FIG9 is a schematic structural diagram of the mixing module in the present application from another angle;

FIG10 is a schematic diagram of the structure of the cleaning module in the present application;

FIG11 is a front view of the cleaning module in the present application;

FIG12 is a schematic diagram of the structure of the top plate in the present application;

FIG13 is a schematic diagram of the structure of the cleaning module in the present application after partially hiding;

FIG14 is a schematic diagram of the structure of the cup sorter in the present application;

FIG. 15 is a top view of the cup organizer in the present application.

Description of the accompanying drawings: 1. box body; 2. reaction cup seat; 3. gun head compartment; 4. reagent compartment; 41. reagent compartment shell; 42. reagent strip; 421. main body; 422. handle; 423. limit hole; 424. clamp; 425. main gear; 5. sample compartment; 51. sample compartment shell; 52. sample strip; 6. waste box; 7. mixing module; 71. support frame; 72. mounting seat; 721. base; 722. top plate; 73. liquid collection device; 731. main body; 732. hanging arm; 733. liquid collection needle; 734. fourth driving mechanism; 735. fifth driving mechanism; 74. oscillation mechanism; 741. mixing drive motor; 742. eccentric shaft; 743. rocker arm; 744. sleeve; 745. sliding bearing; 74 6. Slide; 75. Magnetic attraction mechanism; 751. Magnet; 752. Lifting assembly; 7521. Screw motor; 7522. Magnet seat; 7523. Guide rod; 76. Liquid distribution tank; 77. Cleaning tank; 8. First robotic arm; 9. Second robotic arm; 10. Third robotic arm; 11. First track; 12. Transfer trolley; 13. Cleaning unit; 14. Support seat; 15. Guide rail; 16. First drive mechanism; 17. Second drive mechanism; 18. Third drive mechanism; 20. Second track; 21. Transfer trolley; 22. Swing arm; 23. Testing instrument; 24. Zero position plate; 25. Photoelectric sensor; 26. Cup sorter; 261. Hopper; 262. Slide; 263. Slope; 264. Discharge port; 265. Baffle.

Detailed ways

The present application is further described in detail below in conjunction with Figures 1-15.

An embodiment of the present application discloses a high-speed processor for pre-processing of tubular mass spectrometry samples. Referring to Figures 1 and 2, the high-speed processor for pre-processing of tubular mass spectrometry samples includes a housing 1, in which a reagent compartment 4, a sample compartment 5, a reaction cup holder 2, a gun tip compartment 3, a waste box 6, a mixing module 7, a first robotic arm 8, a second robotic arm 9, a third robotic arm 10, a first track 11 and a transfer cart 12 are installed.

The reagent compartment 4, sample compartment 5, reaction cup holder 2, gun tip compartment 3, waste box 6, mixing module 7 and first track 11 are all installed at the bottom of the box body 1, the first robotic arm 8, the second robotic arm 9 and the third robotic arm 10 are all installed at the top of the box body 1, the sample compartment 5 is located between the reagent compartment 4 and the gun tip compartment 3, the transfer trolley 12 is slidably connected to the first track 11, and the transfer trolley 12 can be moved to the bottom of the first robotic arm 8, the second robotic arm 9 and the third robotic arm 10 respectively. The reagent compartment 4 is used to place reagent tubes containing reagents; the sample compartment 5 is used to place sample tubes containing samples; the reaction cup holder 2 is used to place the reaction cup; the gun tip compartment 3 is used to place the gun tip for aspiration; the waste box 6 is used to place used reaction cups and gun tips; the mixing module 7 is used to mix the reagents and samples evenly, the first robotic arm 8 is used to grab the reaction cup and place the reaction cup on the transfer cart 12, the second robotic arm 9 is used to absorb the reagent and inject the reagent into the reaction cup on the transfer cart 12, and the third robotic arm 10 is used to absorb the sample and inject the sample into the reaction cup on the transfer cart 12.

When pre-processing the sample, the sample tube, reagent tube and gun tip are placed in the corresponding positions in advance, and the transfer trolley 12 is controlled to move to the bottom of the first mechanical arm 8, and the first mechanical arm 8 grabs the reaction cup from the reaction cup holder 2 and places it on the transfer trolley 12; then the transfer trolley 12 moves to the bottom of the second mechanical arm 9, and the second mechanical arm 9 absorbs the reagent and adds it to the reaction cup; then the transfer trolley 12 moves to the bottom of the third mechanical arm 10, and the third mechanical arm 10 takes the gun tip and absorbs the sample, and adds the sample to the reaction cup; the transfer trolley 12 returns to the bottom of the first mechanical arm 8, and the first mechanical arm 8 transfers the reaction cup to the mixing module 7 to achieve reagent and sample mixing, and after purification treatment, to obtain the detection liquid with the target object. In this way, through the cooperation of the first mechanical arm 8, the second mechanical arm 9, the third mechanical arm 10 and the transfer trolley 12, each mechanical arm does not need to move over a large range, which reduces the waiting time, thereby shortening the sample pre-processing time and improving the efficiency of sample pre-processing.

Specifically, the box 1 is preferably a closed type. Since most of the reagents used in the sample pretreatment process are chemical reagents, the chemical reagents will spill into the external environment and cause harm to the human body. Through closed treatment, the harm to the human body during the pretreatment process can be reduced. The material of the box 1 is not specifically limited in this embodiment. Secondly, the box 1 is preferably a rectangular parallelepiped or a cube, which can ensure the maximization of the internal space, so as to facilitate the rational layout of each device.

2 and 3 , the reagent chamber 4 includes a reagent chamber shell 41 and a reagent strip 42 for placing a reagent tube. A plurality of reagent strips 42 are provided, and each reagent strip 42 is inserted from one side of the reagent chamber shell 41 . A through hole is provided at the top of the reagent chamber shell 41 for inserting a pipette needle on the second robotic arm 9 .

4 and 5 , the reagent strip 42 includes a main body 421 and a handle 422. The handle 422 and the main body 421 are integrally formed. A plurality of limiting holes 423 are provided on the main body 421. The plurality of limiting holes 423 are arranged along the length direction of the main body 421. The reagent tube is inserted into the limiting hole 423 to achieve snap-fit fixation of the reagent tube. The handle 422 facilitates the insertion of the reagent strip 42 into the shell of the reagent compartment 4, thereby reducing the difficulty of operation.

Especially, with reference to Fig. 4 and Fig. 5, the end of main body 421 away from handle 422 is connected with clamp 424, and clamp 424 is used for fixing the reagent tube that magnetic bead reagent is housed.Because magnetic bead can be deposited to the bottom of reagent tube under static state, when aspiration needle draws magnetic bead reagent like this, the reagent that draws can be caused to be uneven, therefore need to ensure that the reagent tube that magnetic bead is housed is in motion so that magnetic bead reagent mixes evenly.A driving motor (not shown) is installed on the reagent compartment 4 housing, and main gear 425 is fixedly connected on the output shaft of driving motor, and main gear 425 is located at the bottom of reagent compartment 4, and main gear 425 is meshed with slave gear (not shown), and slave gear sleeve is fixed on the outer wall of reagent tube, and after reagent strip 42 is inserted, main gear 425 and slave gear just can mesh. After fixing the reagent tube on the clamp 424, the reagent strip 42 is inserted into the reagent chamber shell 41 as a whole. At this time, the main gear 425 and the slave gear can just mesh, start the drive motor, and drive the reagent tube containing magnetic beads to rotate so that the magnetic beads will not sink to the bottom of the reagent tube.

Referring to Figures 2 and 4, since multiple reagents need to be added during the pretreatment process, the liquid-pipetting needle on the second mechanical arm 9 needs to be cleaned after completing the filling of one reagent to avoid contaminating other reagents. A cleaning unit 13 is provided on the reagent warehouse 4. When the liquid-pipetting needle on the second mechanical arm 9 completes the filling of the reagent, it will be inserted into the cleaning unit 13, and the cleaning unit 13 will rinse the inner and outer walls of the liquid-pipetting needle. In this way, the second mechanical arm 9 does not need to move over a large range, which improves the convenience during cleaning, and at the same time makes full use of the space of the reagent warehouse 4 to achieve rational use of space.

2 and 3, the sample chamber 5 includes a sample chamber housing 51 and a sample strip 52 for placing a sample tube. A plurality of sample strips 52 are provided, each sample strip 52 is inserted from one side of the sample chamber housing 51, and a through hole is provided at the top of the sample chamber housing 51 for inserting the gun head on the third mechanical arm 10. The structure of the sample strip 52 is the same as that of the reagent strip 42. In this embodiment, the structure of the sample strip 52 is not described again. Of course, the opening size of the sample strip 52 is adapted to the diameter of the sample tube. In this way, each sample tube is inserted into the sample strip 52 in sequence, and then each sample strip 52 is inserted into the sample chamber housing 51 in sequence, so as to facilitate the rapid placement of the sample tube. Of course, according to the needs, the sample chamber housing 51 can also be equipped with a heating device or a cooling device to heat or cool the sample tube. In this embodiment, no specific description is given.

A barcode scanner (not shown in the figure) is also installed on the box body 1. When the sample strip 52 is inserted into the sample chamber shell 51, the barcode scanner can scan the barcode on the sample tube to obtain the sample information. At the same time, the barcode scanner transmits the information to the computer in a timely manner to facilitate the recording of the subsequent test results.

2 and 6, further, a support base 14 is fixed on the box body 1, a horizontally arranged guide rail 15 is fixed on the support base 14, the guide rail 15 is arranged along the length direction of the box body 1, and the first mechanical arm 8, the second mechanical arm 9, and the third mechanical arm 10 are all slidably connected to the guide rail 15. The three mechanical arms move on the same guide rail 15, which ensures the accuracy of the X-axis movement, and also facilitates the installation and positioning of the three mechanical arms. The three mechanical arms are arranged in a colinear manner, which can reduce the installation cost.

The support base 14 is provided with three first driving mechanisms 16, which are arranged one by one with the first mechanical arm 8, the second mechanical arm 9, and the third mechanical arm 10, and are used to drive the first mechanical arm 8, the second mechanical arm 9, and the third mechanical arm 10 to move along the direction in which the guide rail 15 is set. In this way, the three mechanical arms are driven by three separate driving mechanisms, which ensures the independence of the three mechanical arms when working, does not interfere with each other, facilitates coordinated work, and further improves work efficiency.

Of course, the first robotic arm 8, the second robotic arm 9, and the third robotic arm 10 are all controlled by a controller, and the three robotic arms can be controlled by the controller to work simultaneously, and the time can be adjusted to achieve seamless connection of the three robotic arms to maximize efficiency. Since the transfer cart 12 needs to move to a specified position according to the positions of the three robotic arms, it is understandable that there are mutually inductive sensors between each robotic arm and the transfer cart 12, such as infrared sensors, to ensure the accuracy of the moving position of the transfer cart 12.

2 and 6 , the first robotic arm 8 , the second robotic arm 9 , and the third robotic arm 10 have the same structure and can realize movement along the X-axis, Y-axis, and Z-axis. Only the functions of the first robotic arm 8 , the second robotic arm 9 , and the third robotic arm 10 are different. The first robotic arm 8 is connected to a robotic arm capable of clamping a reaction cup, the second robotic arm 9 is connected to a pipette needle, and the third robotic arm 10 is connected to a device capable of removing a gun tip. In this embodiment, only the first robotic arm 8 is taken as an example to briefly explain the structure of the robotic arm.

6, the first robot arm 8 further includes a second drive mechanism 17 and a third drive mechanism 18, wherein the second drive mechanism 17 is used to drive the robot arm to move along the Y-axis direction, and the third drive mechanism 18 is used to drive the robot arm to move along the Z-axis direction. Among them, the first drive mechanism 16, the second drive mechanism 17 and the third drive mechanism 18 all adopt transmission mechanisms well known to those skilled in the art, such as: gear rack, belt drive, lead screw, etc., and no specific description is given in this embodiment. Each robot arm can adopt the structure of a product well known to those skilled in the art, and therefore, the structure of each robot arm is no longer described in detail.

1 and 2 , it should be noted that the first robotic arm 8, the second robotic arm 9, and the third robotic arm 10 need to correspond to the reaction cup holder 2, the reagent compartment 4, and the sample compartment 5, respectively, so as to ensure normal operation. In this embodiment, the sample compartment 5 is preferably located between the reagent compartment 4 and the gun tip compartment 3, that is, the second robotic arm 9 is located between the first robotic arm 8 and the third robotic arm 10. This just meets the pre-processing process, and the transfer trolley 12 can move in sequence, with the shortest moving distance and the highest efficiency.

Referring to Figures 2 and 7, the box body 1 is also equipped with a second track 20, on which a transfer trolley 21 is slidably connected, and a swing arm 22 is installed on the transfer trolley 21. The first track 11 and the second track 20 are parallel. When the sample is pre-processed, the first mechanical arm 8 places the reaction cup on the transfer trolley 12 again, and the transfer trolley 12 and the transfer trolley 21 move to the corresponding positions. The swing arm 22 grabs the reaction cup on the transfer trolley 12, and the transfer trolley 21 sends the reaction cup to the detection instrument 23 (mass spectrometer). In this way, unmanned participation from sample pre-processing to detection is realized, and the processing efficiency is improved. After the sampling test is completed, the swing arm 22 swings to the neutral position, and the third mechanical arm 10 can extract the sample to be tested in the reaction cup, and then the first mechanical arm 8 grabs the reaction cup for recycling, so that the dry and wet separation of waste is realized.

Of course, both the transfer trolley 12 and the transfer trolley 21 are products well known to those skilled in the art. In this embodiment, the specific structures of the transfer trolley 12 and the transfer trolley 21 are not described in detail.

Specifically, referring to Figures 8, 9 and 10, the mixing module 7 includes a support frame 71, a mounting seat 72 mounted on the support frame 71, a liquid taking device 73 mounted on the support frame 71, an oscillating mechanism 74 mounted on the mounting seat 72, and a magnetic suction mechanism 75 mounted on the mounting seat 72. The mounting seat 72, the oscillating mechanism 74 and the magnetic suction mechanism 75 together constitute a cleaning module. In this way, after the reagent and sample are added to the reaction cup, the first mechanical arm 8 transfers the reaction cup to the mounting seat 72, and the oscillating mechanism 74 drives the reaction cup to shake, so that the reagent and sample are fully mixed. At the same time, the target object is adsorbed on the magnetic beads, the magnetic suction mechanism 75 fixes the magnetic beads, and the liquid taking device 73 sucks away the supernatant, and then adds the cleaning liquid for cleaning, and repeats the operation several times to achieve the purification of the target object.

In this embodiment, a plurality of cleaning modules are provided, and the plurality of cleaning modules are spaced apart along the length direction of the support frame 71, and two liquid taking devices are provided, so that a plurality of reaction cups can be processed at the same time, thereby improving the processing efficiency.

Specifically, referring to FIG8 and FIG9, the liquid taking device 73 includes a body 731, a suspension arm 732, a liquid taking needle 733, a fourth driving mechanism 734 and a fifth driving mechanism 735. The fourth driving mechanism 734 is installed on the support frame 71. The fourth driving mechanism 734 is used to drive the body 731 to move along the direction of the support frame 71. The fifth driving mechanism 735 is installed on the body 731. The suspension arm 732 is connected to the body 731. The liquid taking needle 733 is fixed on the suspension arm 732. The fifth driving mechanism 735 is used to drive the suspension arm 732 to rise and fall. In this embodiment, the fourth driving mechanism 734 adopts a belt drive, and the fifth driving mechanism 735 adopts a hydraulic lifting method. Of course, the fourth driving mechanism 734 and the fifth driving mechanism 735 can be replaced by other transmission methods that meet the requirements. In this way, the liquid taking device 73 can be moved to any specified position and complete the corresponding liquid suction work.

8 and 9, the mixing module 7 further includes a liquid preparation tank 76 and a cleaning tank 77, both of which are mounted on the support frame 71. The liquid preparation tank 76 can prepare corresponding cleaning liquid or eluent as needed, and the cleaning tank 77 is used to clean the liquid collection needle 733. In this way, the liquid collection device 73, the liquid preparation tank 76, the cleaning tank 77 and the cleaning unit 13 are integrated together to achieve the purpose of rapid preparation and rapid cleaning, and the range of movement of the liquid collection device 73 is greatly reduced, thereby improving the processing efficiency.

Further, with reference to Figures 10 and 11, the mounting base 72 includes a base 721 and a top plate 722 fixedly connected to the top of the base 721, and the base 721 is L-shaped. The oscillation mechanism 74 includes a mixing drive motor 741 fixed on the top plate 722, the mixing drive motor 741 is a through-type drive motor, the output shaft of the mixing drive motor 741 runs through the body 731 of the mixing drive motor 741, the output shaft of the mixing drive motor 741 is vertically arranged, the upper end of the output shaft of the mixing drive motor 741 is fixedly connected with an eccentric shaft 742, and a rocker arm 743 is fixed on the eccentric shaft 742, the rocker arm 743 can swing back and forth on the top plate 722, and a through hole for inserting a reaction cup is provided on the rocker arm 743. Start the mixing drive motor 741 to drive the eccentric shaft 742 to rotate, and the reaction cup can be shaken to achieve oscillation and mixing of reagents and samples during the cleaning process.

10 and 11 , a hollow sleeve 744 is connected to the bottom of the rocker arm 743, and the sleeve 744 is passed through the top plate 722. On the one hand, the sleeve 744 can protect the reaction cup, and on the other hand, the sleeve 744 increases the weight of the rocker arm 743 and reduces the swing amplitude to prevent the solution in the reaction cup from overflowing due to excessive shock force.

11 and 12, a sliding bearing 745 is provided at the bottom of the swing arm 22, and a slide groove 746 for the sliding bearing 745 to slide is opened on the top plate 722. When the mixing drive motor 741 drives the swing arm 743 to shake, the sliding bearing 745 can absorb the force between the swing arm 743 and the top plate 722 to improve the stability of the swing arm 743 during movement.

Of course, referring to Fig. 13, a corresponding detection device or sensor is installed on the mounting seat 72 to realize automatic control of the oscillation process, which will not be described in detail in this embodiment. In this embodiment, a zero position disk 24 and a photoelectric sensor 25 are used.

10 and 13, the magnetic attraction mechanism 75 includes a magnet 751 disposed on the mounting seat 72 and a lifting assembly 752 disposed on the mounting seat 72, and the lifting assembly 752 is used to drive the magnet 751 to rise or fall along the axis direction of the reaction cup. When the cleaning solution is added to the reaction cup, the lifting assembly 752 drives the magnet 751 to move away from the reaction cup, and the attraction between the magnet 751 and the magnetic beads is continuously reduced. At this time, the oscillation mechanism 74 drives the reaction cup to shake, so that the magnetic beads and the cleaning solution are fully mixed. In this way, the magnetic beads can be fully mixed with the cleaning solution during the magnetic bead cleaning process to reduce the non-target substance residue on the magnetic beads and increase the concentration of the target substance.

The lifting assembly 752 includes a screw motor 7521 fixed to the bottom of the mounting seat 72, the output shaft of the screw motor 7521 is vertically arranged, a nut (not shown in the figure) is threadedly connected to the output shaft of the screw motor 7521, the nut is fixedly connected to the magnet seat 7522, the magnet 751 is arranged in the magnet seat 7522, a guide rod 7523 is fixedly connected between the base 721 and the top plate 722, and the guide rod 7523 is penetrated on the magnet 751 seat. When the screw motor 7521 is started, the output shaft of the screw motor 7521 drives the magnet 751 seat to rise or fall through the nut and the guide rod 7523, thereby achieving the rise or fall of the magnet 751.

13, two guide rods 7523 are provided, and both guide rods 7523 are parallel to the output shaft of the screw motor 7521, and the two guide rods 7523 are symmetrically arranged about the output shaft of the screw motor 7521. In this way, by providing two guide rods 7523, the stability of the magnet seat 751 during movement can be improved.

The magnet seat 7522 is U-shaped, and the reaction cup can be inserted into the opening of the magnet seat 7522. Two magnets 751 are provided. When the reaction cup is located at the opening of the magnet seat 7522, the two magnets 751 are located on both sides of the reaction cup to enhance the attraction to the magnetic beads, so that the magnetic beads can be adsorbed on the tube wall of the reaction cup, thereby reducing the magnetic loss rate.

Of course, the screw motor 7521 is also controlled by the controller to achieve the rise or fall of the magnet 751. In this embodiment, the sensor used is the photoelectric sensor 25.

2 , in order to further improve the degree of automation and solve the problem of manually placing reaction cups, a cup sorter 26 is further provided in the box body 1. After the reaction cups are poured into the cup sorter 26, the cup sorter 26 can neatly arrange the reaction cups according to actual requirements and transport the reaction cups to the reaction cup holder 2 for use, thereby greatly improving convenience.

14 and 15, the cup sorter 26 includes a hopper 261, a slide 262 and a conveying mechanism (not shown in the figure). A slope 263 is formed in the hopper 261, and a discharge port 264 is provided on the hopper 261. The slide 262 is located just below the discharge port 264. After the reaction cup is placed in the hopper 261, the reaction cup slides out of the discharge port 264 under the action of the slope 263 and falls on the slide 262. Since a cup brim is formed on the top of the reaction cup, the reaction cup can be stuck on the slide 262 and slide to the bottom of the slide 262 under the action of gravity. A transfer channel (not shown in the figure) is also connected to the bottom of the slide 262. The conveying mechanism transfers the reaction cup to the reaction cup holder 2 through the transfer channel. In this way, the automatic stacking and transfer of the reaction cups are realized.

14 and 15 , the cup sorter 26 further includes a lifting mechanism (not shown in the figure) and a baffle 265. The baffle 265 is vertically arranged on the hopper 261. The lifting mechanism drives the baffle 265 to be lifted and lowered. The baffle 265 is arranged near the discharge port 264. When the top of the reaction cup slides out of the discharge port 264 first, the reaction cup cannot fall into the slideway 262. The lifting mechanism drives the baffle 265 to rise and push the reaction cup back. Under the action of the slope 263, the reaction cup changes direction and falls smoothly into the slideway 262.

Of course, a plurality of sensors are installed on the cup sorter 26 to ensure the normal and continuous operation. In this embodiment, no specific description is given. The conveying mechanism and the lifting mechanism are both mechanisms known to those skilled in the art and only need to meet the requirements. In this embodiment, no specific description or limitation is given to the conveying mechanism and the lifting mechanism.

2 , the waste box 6 is a box-type structure, and the waste box 6 can be placed at a suitable position inside the box body 1 as needed. In the present embodiment, two waste boxes 6 are provided, one of which is installed on one side of the gun tip bin 3 for collecting used gun tips, and the other waste box 6 is installed on one side of the cup sorter 26 for collecting used reaction cups.

The implementation principle of a high-speed processor for pre-processing of tubular mass spectrometry samples in an embodiment of the present application is as follows: when pre-processing the sample, the sample tube is placed in the sample compartment 5 in advance, the reagent tube is placed in the reagent compartment 4, the gun tip is placed in the gun tip compartment 3, and the reaction cup is placed in the cup sorter 26. The cup sorter 26 organizes the reaction cups as required and transfers them to the reaction cup holder 2. The transfer cart 12 moves to the bottom of the first robotic arm 8. The first robotic arm 8 grabs the reaction cup from the reaction cup holder 2 and places it on the transfer cart 12. The transfer cart 12 then moves to the bottom of the second robotic arm 9. The second robotic arm 9 absorbs the reagent and adds it to the reaction cup. The transfer cart 12 then moves to the bottom of the third robotic arm 10. The third robotic arm 10 takes the gun tip and absorbs the sample and adds the sample to the reaction cup. The transfer trolley 12 moves to the bottom of the first mechanical arm 8, and the first mechanical arm 8 transfers the reaction cup to the mixing module 7. The oscillation mechanism 74 mixes the reagent and the sample, and the lifting component 752 drives the magnet 751 to rise to magnetically absorb the magnetic beads. The liquid taking device 73 takes the supernatant, and the liquid preparation pool 76 is configured with a cleaning liquid. The liquid taking device 73 adds the cleaning liquid to the reaction cup, and the lifting component 752 drives the magnet 751 to descend. At the same time, the oscillation mechanism 74 mixes the magnetic beads and the cleaning liquid, and then magnetically absorbs the supernatant. After multiple cleanings, an eluent is added to the reaction cup to separate the target and the magnetic beads, thereby obtaining a high-purity target. The sample reaction cup that has been pre-treated is placed on the transfer trolley 12 again, and the transfer trolley 21 grabs the reaction cup on the transfer trolley 12, and then sends the reaction cup to the detection instrument 23 for detection. After the detection is completed, the transfer trolley 21 returns to drain the remaining liquid in the reaction cup, and the first mechanical arm 8 puts the used reaction cup into the waste box 6. In this way, through the cooperation of the first robotic arm 8, the second robotic arm 9, the third robotic arm 10 and the transfer cart 12, the first robotic arm 8, the second robotic arm 9, and the third robotic arm 10 only need to complete the tasks of taking the cup, adding reagents and adding samples respectively, and each robotic arm no longer needs to move over a large range, which greatly shortens the time for taking the cup, aspirating liquid and injecting liquid, thereby shortening the time for sample pretreatment and improving the efficiency of sample pretreatment.

The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims (10)

1. A high-speed processor for pretreatment of a tubular mass spectrum sample, which is characterized in that: the device comprises a box body (1), a reagent bin (4), a sample bin (5), a reaction cup seat (2), a gun head bin (3), a waste box (6), a mixing module (7), a first mechanical arm (8), a second mechanical arm (9), a third mechanical arm (10), a first track (11) and a transfer trolley (12);

The reagent bin (4) is arranged in the box body (1) and is used for placing a reagent tube filled with a reagent;

The sample bin (5) is arranged in the box body (1) and is used for placing a sample tube filled with a sample;

the reaction cup seat (2) is arranged in the box body (1) and is used for placing a reaction cup;

The gun head bin (3) is arranged in the box body (1) and used for placing a gun head for absorbing liquid, and the third mechanical arm (10) absorbs the gun head before absorbing liquid;

the waste box (6) is arranged in the box body (1) and is used for placing used reaction cups and gun heads;

the mixing module (7) is arranged in the box body (1) and comprises a support frame (71), an oscillating mechanism (74) arranged on the support frame (71) and a liquid taking device (73) arranged on the support frame (71), wherein the oscillating mechanism (74) is used for uniformly mixing a reagent and a sample, and the liquid taking device (73) is used for sucking supernatant in a reaction cup;

The first mechanical arm (8), the second mechanical arm (9), the third mechanical arm (10), the first track (11) and the transfer trolley (12) are all arranged in the box (1), the transfer trolley (12) is slidably connected to the first track (11), the transfer trolley (12) can be respectively moved to the lower parts of the first mechanical arm (8), the second mechanical arm (9) and the third mechanical arm (10), the first mechanical arm (8) is used for grabbing a reaction cup and placing the reaction cup on the transfer trolley (12), the second mechanical arm (9) is used for sucking a reagent and injecting the reagent into the reaction cup on the transfer trolley (12), and the third mechanical arm (10) is used for sucking a sample and injecting the sample into the reaction cup on the transfer trolley (12).

2. The high-speed processor for pretreatment of a tubular mass spectrometry sample according to claim 1, wherein: still including installing supporting seat (14) on box (1) and install guide rail (15) on supporting seat (14), guide rail (15) are followed the length direction setting of box (1), first arm (8) second arm (9) third arm (10) all sliding connection is in on guide rail (15).

3. The high-speed processor for pretreatment of a tubular mass spectrometry sample according to claim 2, wherein: be provided with three first actuating mechanism (16) on supporting seat (14), three first actuating mechanism (16) with first arm (8) second arm (9) third arm (10) one-to-one sets up, is used for driving first arm (8) second arm (9) third arm (10) are followed guide rail (15) set up the direction and are removed.

4. The high-speed processor for pretreatment of a tubular mass spectrometry sample according to claim 1, wherein: still include second track (20) and sliding connection are in transfer dolly (21) on second track (20), install swing arm (22) on transferring dolly (21), first track (11) with second track (20) are parallel, swing arm (22) can snatch the reaction cup on transfer dolly (12), transfer dolly (21) are arranged in sending the reaction cup to detecting instrument (23).

5. The high-speed processor for pretreatment of a tubular mass spectrometry sample according to claim 1, wherein: the reaction cup placed in the mixing module (7) is provided with magnetic beads capable of adsorbing target substances, and the mixing module (7) further comprises a magnetic attraction mechanism (75) arranged on the supporting frame (71) and used for fixing the positions of the magnetic beads in the reaction cup.

6. The high-speed processor for pretreatment of a tubular mass spectrometry sample according to claim 5, wherein: the magnetic attraction mechanism (75) comprises a mounting seat (72), a magnet (751) arranged on the mounting seat (72) and a lifting assembly (752) arranged on the mounting seat (72), and the lifting assembly (752) is used for driving the magnet (751) to lift or descend along the axial direction of the reaction cup.

7. The high-speed processor for pretreatment of a tubular mass spectrometry sample according to claim 1, wherein: the liquid taking device is characterized in that a liquid distribution tank (76) and a cleaning tank (77) are further arranged on the supporting frame (71), the liquid taking device (73) comprises a liquid taking needle (733), the liquid distribution tank (76) is used for preparing a solution required in a sample purifying process so as to be sucked by the liquid taking needle (733), and the cleaning tank (77) is used for cleaning the liquid taking needle (733).

8. The high-speed processor for pretreatment of a tubular mass spectrometry sample according to claim 1, wherein: install driving motor on reagent storehouse (4), the rigid coupling has master gear (425) on driving motor's the output shaft, master gear (425) are located the bottom in reagent storehouse (4), master gear (425) meshing has from the gear, from the gear can overlap to establish to be fixed on the outer wall of reagent pipe, when the reagent pipe is followed after reagent storehouse (4) inserts, master gear (425) with from the gear just can mesh.

9. The high-speed processor for pretreatment of a tubular mass spectrometry sample according to claim 1, wherein: the reagent bin (4) is provided with a cleaning unit (13) for cleaning the liquid suction needle on the second mechanical arm (9).

10. The high-speed processor for pretreatment of a tubular mass spectrometry sample according to claim 1, wherein: the cup arranging device (26) is used for arranging the reaction cups and conveying the reaction cups to the reaction cup seat (2).