CN108865659A - Nucleic acid extraction and amplification fluorescent detection system - Google Patents
Nucleic acid extraction and amplification fluorescent detection system Download PDFInfo
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- CN108865659A CN108865659A CN201811093908.6A CN201811093908A CN108865659A CN 108865659 A CN108865659 A CN 108865659A CN 201811093908 A CN201811093908 A CN 201811093908A CN 108865659 A CN108865659 A CN 108865659A
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- 238000001514 detection method Methods 0.000 title claims abstract description 96
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 74
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 74
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 73
- 238000000605 extraction Methods 0.000 title claims abstract description 57
- 230000003321 amplification Effects 0.000 title claims abstract description 53
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 53
- 238000007781 pre-processing Methods 0.000 claims abstract description 29
- 230000003416 augmentation Effects 0.000 claims abstract description 25
- 238000005070 sampling Methods 0.000 claims abstract description 20
- 238000004458 analytical method Methods 0.000 claims abstract description 9
- 239000011324 bead Substances 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims description 24
- 238000003860 storage Methods 0.000 claims description 17
- 239000002699 waste material Substances 0.000 claims description 16
- 230000007246 mechanism Effects 0.000 claims description 14
- 239000012085 test solution Substances 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 13
- 239000006148 magnetic separator Substances 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000001917 fluorescence detection Methods 0.000 claims description 3
- 230000000712 assembly Effects 0.000 claims description 2
- 238000000429 assembly Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 abstract description 96
- 238000003745 diagnosis Methods 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 4
- 239000012472 biological sample Substances 0.000 abstract description 3
- 239000010808 liquid waste Substances 0.000 abstract description 3
- 239000000758 substrate Substances 0.000 description 11
- 230000032258 transport Effects 0.000 description 9
- 238000010586 diagram Methods 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 210000000078 claw Anatomy 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000000007 visual effect Effects 0.000 description 4
- 238000009413 insulation Methods 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
The present invention relates to biological sample analysis detection fields, in particular to a kind of nucleic acid extraction and amplification fluorescent detection system.The nucleic acid extraction includes with amplification fluorescent detection system：Sample sampling device, kit conveyer, sample preprocessing device, nucleic acid-extracting apparatus, augmentation detection device and mechanical arm system.Magnetic bead sample is extracted and handled by the way that nucleic acid-extracting apparatus is arranged, realizes high-throughput signal detection, the degree of automation with higher by the way that augmentation detection device is arranged.By the way that sample sample introduction, kit transport, sample preprocessing, nucleic acid extraction, augmentation detection, each function of liquid waste processing are integrated into a system, integrated degree is high.Each functional module of the nucleic acid extraction and amplification fluorescent detection system is rationally distributed, it is compact-sized, entire nucleic acid extraction and amplification fluorescent detection system have the characteristics that miniaturization, can adapt to the requirement of Personalized medicine, are applied to various medical institutions' occasions and in time diagnosis etc..
Description
Technical field
The present invention relates to biological sample analysis detection fields, examine in particular to a kind of nucleic acid extraction and amplification fluorescent
Examining system.
Background technique
Biological sample analysis detection needs high degree of automation, integrated degree high and the demand of the diagnostic system of miniaturization
Urgently.
" automation " refers to that fully automatic molecular diagnostic system can complete " sample preparation-target molecule enrichment or amplification-signal
The full process automatization of the biomolecule detection analysis of detection-data analysis ".
" integration " refers to be required to store in sample by automation equipment, sample preparation, analysis reaction, signal detection
It is integrated with functions such as analyses.
" miniaturization " refers to the miniaturization of fully automatic molecular diagnostic system to adapt to the requirement of Personalized medicine, to be applied to respectively
A medical institutions' occasion and in time diagnosis etc..
Summary of the invention
The purpose of the present invention is to provide a kind of nucleic acid extraction and amplification fluorescent detection system, high degree of automations, one
Change degree is high and minimizes.
To achieve the goals above, technical solution used in the embodiment of the present invention is as follows：
A kind of nucleic acid extraction and amplification fluorescent detection system, including：Sample sampling device, sample sampling device include multiple
For placing the sample rack and sample conveying mechanism of specimen bottle；Kit conveyer, kit conveyer include multiple
Kit storage assembly and kit conveying mechanism；Sample preprocessing device, sample preprocessing device are used for sample and examination
It heated, stirred and centrifugal treating after agent mixing；Nucleic acid-extracting apparatus, nucleic acid-extracting apparatus are used for sample using extraction examination
Agent carries out nucleic acid extraction and Magnetic Isolation；Augmentation detection device, sample of the augmentation detection device for will be handled by nucleic acid
Amplification and fluorimetric analysis are carried out after microcosmic chip is made；And mechanical arm system, mechanical arm system be used for sample or
Reagent is transported to sample sampling device, kit conveyer, sample preprocessing device, nucleic acid-extracting apparatus or augmentation detection
Device.
In preferred embodiments of the present invention, kit storage assembly include it is multiple for store the accommodating cavities of kit with
And cartridge clip shell；A cartridge clip shell is placed in each accommodating cavity；Multiple kits overlay in cartridge clip shell.
In preferred embodiments of the present invention, kit conveying mechanism includes gripper assembly, moving track and driving group
Part；Gripper assembly is used to take out kit from cartridge clip shell inner clip；Gripper assembly transmission connection is in driving assembly, to drive folder
Component is taken to move along moving track.
In preferred embodiments of the present invention, sample preprocessing device includes the turntable and absorption group for placing kit
Part；Pick-up component is connected to turntable, and is located above the turntable, pick-up component be able to enter in kit draw test solution or
Stirring.
In preferred embodiments of the present invention, the bottom of turntable is provided with heating component, for the test solution in kit
Heating.
In preferred embodiments of the present invention, augmentation detection device includes detection device, microcosmic chip and chip movement
Component；Microcosmic chip is for accommodating multiple samples to be detected；Detection device includes heat circulating system and live signal detection system
System, heat circulating system are used to carry out nucleic acid amplification for the sample in microcosmic chip to provide suitable temperature；Live signal detection system
It unites for carrying out fluorescence detection to the sample after generation nucleic acid amplification；Chip movement component is used to grab microcosmic chip and will be microcosmic
Chip is transferred to the detection zone of detection device.
In preferred embodiments of the present invention, nucleic acid-extracting apparatus includes magnetic separator, nucleic acid extracting reagent storage, adds
Hot device assembly and liquid relief component；Magnetic separator is used for test solution and Beads enrichment；Nucleic acid extracting reagent storage is for storing
From one or more reagents of sample extraction nucleic acid；Heater assembly, which is used to provide nucleic acid extraction reaction, is suitble to temperature.
In preferred embodiments of the present invention, nucleic acid extraction and amplification fluorescent detection system further include the public liquid box of waste liquid
Son；The public liquid box of waste liquid is arranged between nucleic acid-extracting apparatus and sample sampling device.
In preferred embodiments of the present invention, it is provided on each sample rack multiple for placing the accommodating of specimen bottle
Chamber；Sample conveying mechanism includes driving portion and moving guide rail；Driving portion is sequentially connected in moving guide rail；Each sample rack connects
It is connected to moving guide rail.
In preferred embodiments of the present invention, mechanical arm system includes drawing arm and crawl arm；Arm is drawn for drawing examination
Liquid and the position for being moved to needs；Crawl arm is used to grab test solution box and is moved to the position of needs.
The beneficial effects of the invention are as follows：
A kind of nucleic acid extraction provided by the invention and amplification fluorescent detection system, including：Sample sampling device, kit fortune
Send device, sample preprocessing device, nucleic acid-extracting apparatus, augmentation detection device and mechanical arm system.Wherein, sample sample introduction fills
It sets including multiple for placing the sample rack and sample conveying mechanism of specimen bottle.Kit conveyer includes multiple kits
Storage assembly and kit conveying mechanism.Sample preprocessing device after mixing sample with reagent for being heated, being stirred
And centrifugal treating.Nucleic acid-extracting apparatus is used to carry out nucleic acid extraction and Magnetic Isolation using reagent is extracted to sample.Amplification inspection
Device is surveyed for amplification and fluorimetric analysis will to be carried out after microcosmic chip is made in the sample that nucleic acid is handled.Mechanical arm system
It is filled for transporting sample or reagent to sample sampling device, kit conveyer, sample preprocessing device, nucleic acid extraction
It sets or augmentation detection device.The nucleic acid extraction and amplification fluorescent detection system are by being arranged nucleic acid-extracting apparatus to magnetic bead sample
It extracts and handles, realize high-throughput signal detection, the degree of automation with higher by the way that augmentation detection device is arranged.
The nucleic acid extraction and amplification fluorescent detection system examine sample sample introduction, kit transport, sample preprocessing, nucleic acid extraction, amplification
It surveys, each function of liquid waste processing is integrated into a system, integrated degree height.The nucleic acid extraction and amplification fluorescent detection system
Each functional module is rationally distributed, compact-sized, and entire nucleic acid extraction and amplification fluorescent detection system have the characteristics that miniaturization,
It can adapt to the requirement of Personalized medicine, be applied to various medical institutions' occasions and in time diagnosis etc..
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the structural schematic diagram of the nucleic acid extraction that first embodiment of the invention provides and amplification fluorescent detection system；
Fig. 2 is the enlarged drawing in Fig. 1 at II；
Fig. 3 is the kit conveyer of the nucleic acid extraction that first embodiment of the invention provides and amplification fluorescent detection system
The first visual angle structural schematic diagram；
Fig. 4 is the kit conveyer of the nucleic acid extraction that first embodiment of the invention provides and amplification fluorescent detection system
The second visual angle structural schematic diagram；
Fig. 5 is the kit conveyer of the nucleic acid extraction that first embodiment of the invention provides and amplification fluorescent detection system
Cartridge clip shell structural schematic diagram；
Fig. 6 is the kit conveyer of the nucleic acid extraction that first embodiment of the invention provides and amplification fluorescent detection system
Sample preprocessing device the first visual angle structural schematic diagram；
Fig. 7 is the kit conveyer of the nucleic acid extraction that first embodiment of the invention provides and amplification fluorescent detection system
Sample preprocessing device the second visual angle structural schematic diagram；
Fig. 8 is the kit conveyer of the nucleic acid extraction that first embodiment of the invention provides and amplification fluorescent detection system
Augmentation detection device structural schematic diagram；
Fig. 9 is the enlarged drawing in Fig. 1 at Ⅸ；
Figure 10 is the kit transport dress of the nucleic acid extraction that first embodiment of the invention provides and amplification fluorescent detection system
The structural schematic diagram for the augmentation detection device set.
Icon：10- nucleic acid extraction and amplification fluorescent detection system；20- substrate；The first side 21-；22- second side；23-
Three sides；The 4th side 24-；100- sample sampling device；110- sample rack；120- sample conveying mechanism；121- driving portion；122- is moved
Dynamic guide rail；200- kit conveyer；210- kit storage assembly；211- accommodating cavity；212- cartridge clip shell；213- reagent
Box；220- kit conveying mechanism；221- gripper assembly；222- moving track；223- driving assembly；300- sample preprocessing dress
It sets；310- turntable；320- pick-up component；330- heating component；331- heat conduction ring；The heating tape 332-；333- thermal insulation layer；400- expands
Increase detection device；410- detection device；411- heat circulating system；412- live signal detection system；The microcosmic chip of 420-；430-
Chip movement component；500- nucleic acid-extracting apparatus；510- bracket；520- fixator；530- magnetic separator；600- waste liquid is public
Liquid box；610- first layer holding part；620- second layer holding part；630- agitating device；611- chamber；710- draws arm；
711- first draws arm；712- second draws arm；713- third draws arm；714- aspirating needle group；715- liquid needle ontology；716- peace
Loading board；720- crawl arm；The first crawl arm of 721-；723- third crawl arm；800- display system.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented
The component of example can be arranged and be designed with a variety of different configurations.
Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below claimed
The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
It should be noted that：Similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
In the description of the embodiment of the present invention, it should be noted that the orientation of the instructions such as term " on ", "inner" or position are closed
System for be based on the orientation or positional relationship shown in the drawings or the invention product using when the orientation usually put or position close
System, is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must have
Specific orientation is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.
In the description of the present invention, it is also necessary to which explanation is unless specifically defined or limited otherwise, term " setting ",
" installation ", " connected ", " connection " shall be understood in a broad sense, for example, it may be fixedly connected, may be a detachable connection or one
Connect to body；It can be directly connected, the connection inside two elements can also be can be indirectly connected through an intermediary.
For the ordinary skill in the art, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
Fig. 1-Figure 10 is please referred to, the present embodiment provides a kind of nucleic acid extractions and amplification fluorescent detection system 10 comprising sample
This sampling device 100, kit conveyer 200, sample preprocessing device 300, augmentation detection device 400, nucleic acid extraction dress
Set the public liquid box 600 of 500, waste liquid and mechanical arm system.The nucleic acid extraction and amplification fluorescent detection system 10 pass through setting
Nucleic acid-extracting apparatus 500 is extracted and is handled to magnetic bead sample, realizes high-throughput letter by the way that augmentation detection device 400 is arranged
Number detection, the degree of automation with higher.The nucleic acid extraction and amplification fluorescent detection system 10 transport sample sample introduction, kit
It send, sample preprocessing, nucleic acid extraction, augmentation detection, each function of liquid waste processing are integrated into a system, integrated degree height.
Each functional module of the nucleic acid extraction and amplification fluorescent detection system 10 is rationally distributed, compact-sized, entire nucleic acid extraction with
Amplification fluorescent detection system 10 has the characteristics that miniaturization, can adapt to the requirement of Personalized medicine, is applied to various therapeutic machines
Structure occasion and in time diagnosis etc..
Further, nucleic acid extraction and amplification fluorescent detection system 10 have substrate 20, sample sampling device above-mentioned
100, kit conveyer 200, sample preprocessing device 300, nucleic acid-extracting apparatus 500, augmentation detection device 400, waste liquid
Public liquid box 600 and mechanical arm system are arranged on the substrate 20.
Further, in the present embodiment, the shape of substrate 20 is rectangular.Substrate 20 has the first side 21 and opposite the
Two sides 22；And third side 23 and the 4th opposite side 24.Sample preprocessing device 300 and kit conveyer 200 are arranged
In the first side 21 and sample preprocessing device 300 is close to third side 23.Kit is transported to sample preprocessing dress to facilitate
It sets at 300.Further, sample sampling device 100 is arranged in third side 23, and is located remotely from sample preprocessing device 300
One end, to facilitate sample transport to far from sample preprocessing device 300.Further, nucleic acid-extracting apparatus 500 is arranged
Among substrate 20, so that convenience sample sampling device 100 transports sample, kit conveyer 200 transports reagent and sample
This pretreatment unit 300 is conveyed through the sample of processing.Further, the setting of augmentation detection device 400 is in second side 22, conveniently
In sample transport to augmentation detection device 400 on nucleic acid-extracting apparatus 500.
Further, kit conveyer 200 includes multiple kit storage assemblies 210 and kit conveying mechanism
220。
Further, kit storage assembly 210 includes multiple for storing the accommodating cavity 211 and cartridge clip shell of kit
Body 212；A cartridge clip shell 212 is placed in each accommodating cavity 211；Multiple kits 213 overlay cartridge clip shell 212
It is interior.Further, kit conveying mechanism 220 includes gripper assembly 221, moving track 222 and driving assembly 223.Into one
Step ground, gripper assembly 221 are used to take out kit 213 from 212 inner clip of cartridge clip shell；Gripper assembly 221 is sequentially connected in drive
Dynamic component 223, to drive gripper assembly 221 to move along moving track 222.
Further, sample preprocessing device 300 for heated after mixing sample with reagent, stirred and centrifugation at
Reason.Specifically, sample preprocessing device 300 includes the turntable 310 and pick-up component 320 for placing kit；Pick-up component
320 are connected to turntable 310, and are located at the top of turntable 310, pick-up component 320 be able to enter in kit draw test solution or
Person's stirring.Further, the bottom of turntable 310 is provided with heating component 330, for heating to the test solution in kit.Turntable
The holding part of multiple accommodating kits is provided on 310.Cooperate during turntable 310 rotates with pick-up component 320 and carries out
Stirring.
Further, heating component 330 includes heat conduction ring 331, heating tape 332 and thermal insulation layer 333.Each holding part
Bottom is provided with heat conduction ring 331；Heating tape 332 is arranged under heat conduction ring 331.The bottom wall of heating tape 332 and turntable 310 it
Between be additionally provided with thermal insulation layer 333.So as to avoid the diffusion of heat, entire 300 structure of sample preprocessing device is further increased
Reliability.
Further, augmentation detection device 400 is used to mention to by pretreated sample using reagent progress nucleic acid is extracted
It takes and Magnetic Isolation.Specifically, augmentation detection device 400 includes detection device 410, microcosmic chip 420 and chip movement
Component 430.Microcosmic chip 420 is for accommodating multiple samples to be detected.Detection device 410 is including heat circulating system 411 and in fact
When signal detection system 412, heat circulating system 411 be used for in microcosmic chip 420 sample progress nucleic acid amplification provide properly
Temperature.Live signal detection system 412 is used to carry out fluorescence detection to the sample after generation nucleic acid amplification.Chip movement component
430 are used to grab microcosmic chip 420 and microcosmic chip 420 are transferred to the detection zone of detection device 410.
Further, nucleic acid-extracting apparatus 500 includes magnetic separator 530, nucleic acid extracting reagent storage, heater assembly
And liquid relief component.Magnetic separator 530 is used for test solution and Beads enrichment；Nucleic acid extracting reagent storage is for storing from sample
Extract one or more reagents of nucleic acid；Heater assembly, which is used to provide nucleic acid extraction reaction, is suitble to temperature.Specifically, above-mentioned
Magnetic separator 530, nucleic acid extracting reagent storage, heater assembly be arranged on multiple brackets 510, be respectively configured to connect
By the sample of certain amount and the fixator 520 of respective number.Wherein each fixator 520 include process chamber, waste chamber, one
Or multiple pipette tips, and one or more storages.Wherein one or more storages are used to carry out containing sufficient amount respectively
From one or more reagents of sample extraction nucleic acid.It is configured to the magnetism mobile relative to the process chamber of each fixator 520 point
From device 530.It is configured to the heater assembly of each process chamber of independent heating.Be configured in two or more fixators 520
Liquid distributor etc. that is upper while carrying out fluid transmitting operation.
Further, the public liquid box 600 of waste liquid is arranged in the nucleic acid-extracting apparatus 500 and sample sampling device 100
Between.Specifically, the public liquid box 600 of waste liquid includes first layer holding part 610, second layer holding part 620 and agitating device
630.Wherein, first layer holding part 610 includes multiple mutually disconnected chambers 611.Each chamber 611 all has at least one
A entrance.At least one chamber 611 is waste collection chamber.Second layer holding part 620 is arranged under first layer holding part 610.
Second layer holding part 620 is configurable for holding waste liquid.Each waste collection chamber is connected to second layer holding part 620.
Agitating device 630 is connected to waste collection chamber.Multiple chambers 611 of the public liquid box 600 of the waste liquid and agitating device 630
Space layout compact and reasonable facilitates the use of operator.Public reagent holding part and waste liquid are set simultaneously in a box
Collection portion not only saves space, but also reduces the number that user replaces consumptive material, has saved the time, has improved service efficiency.
The setting of agitating device 630 solves the problems, such as that magnetic bead precipitating, using effect are more preferable.
Further, sample sampling device 100 includes multiple sample racks 110 for placing specimen bottle and sample conveying
Mechanism 120.Further, it is provided on each sample rack 110 multiple for placing the accommodating cavity of specimen bottle；Sample conveying
Mechanism 120 includes driving portion 121 and moving guide rail 122；Driving portion 121 is sequentially connected in moving guide rail 122；Each sample rack
110 are all connected to moving guide rail 122.Specifically, in the present embodiment, sample sampling device 100 include 4 row's sample racks 110 into
Sample, every row's sample rack 110 can store 12 specimen bottles.
Further, mechanical arm system is used to transport in sample or reagent to sample sampling device 100, kit transport
At device 200, sample preprocessing device 300, nucleic acid-extracting apparatus 500 or augmentation detection device 400.Further, mechanical
Arm system includes drawing arm 710 and crawl arm 720.Further, arm 710 is drawn to be used to draw test solution and be moved to the position of needs
It sets；Crawl arm 720 is used to grab test solution box and is moved to the position of needs.Specifically, in the present embodiment, arm 710 is drawn to wrap
It includes and draws arm 711, along the setting of 20 second side of substrate, 22 extending direction along the first of the setting of 20 first side of substrate, 21 extending direction
Second absorption arm 712, the third being slidably arranged between the first absorption arm 711 and the second absorption arm 712 are drawn arm 713 and are inhaled
Liquid needle group 714.Aspirating needle group 714 is slidably connected to third and draws arm 713, can draw in third and move back and forth on arm 713, together
When third draw arm 713 second absorption arm 712 and first absorption arm 711 on slide when, can be realized aspirating needle group 714 and exist
The movement of X and Y both direction.Further, aspirating needle group 714 includes aspirating needle ontology 715 and mounting plate 716, aspirating needle sheet
Body 715 is slidably connected to mounting plate 716, to realize aspirating needle ontology 715 in the movement of Z-direction, so that entirely
Drawing arm 710 can move in the range of entire substrate 20.
Further, the inside for drawing arm 710 is arranged in crawl arm 720, and height is lower than crawl arm 720.Crawl arm 720
It is mainly used for grabbing kit.Crawl arm 720 includes the first crawl arm 721, the second crawl arm (not shown), third crawl arm
723 and clip claw assembly (not shown).First crawl arm 721 is connected on the backboard of the 4th side 24 of substrate 20 setting, and along the
The extending direction of four sides 24 is arranged；Second crawl arm is arranged along the direction of the first side 21, and is vertically connected at the first crawl arm
721, third crawl arm 723 is connected to the first crawl arm 721 along the direction of 20 plane of vertical substrate.Clip claw assembly is slidably connected to
Second crawl arm, to clamp kit when clip claw assembly can be moved along tri- directions XYZ.
It should be understood that the specific structure of above-mentioned clip claw assembly can choose this field it is common can be used in clamp kit
Gripping structure.
Further, the nucleic acid extraction and amplification fluorescent detection system 10 are additionally provided with display system 800, so as to aobvious
Show detection data.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.It should be noted that：Similar label and letter exist
Similar terms are indicated in following attached drawing, therefore, once being defined in a certain Xiang Yi attached drawing, are then not required in subsequent attached drawing
It is further defined and explained.
Claims (10)
1. a kind of nucleic acid extraction and amplification fluorescent detection system, which is characterized in that including：
Sample sampling device, the sample sampling device include multiple for placing the sample rack and sample conveyer of specimen bottle
Structure；
Kit conveyer, the kit conveyer include multiple kit storage assemblies and kit conveyer
Structure；
Sample preprocessing device, the sample preprocessing device for heated after mixing sample with reagent, stirred and from
Heart processing；
Nucleic acid-extracting apparatus, the nucleic acid-extracting apparatus are used to carry out nucleic acid extraction and magnetic point using reagent is extracted to sample
From；
Augmentation detection device, the augmentation detection device are used to microcosmic chip will be made by the sample of nucleic acid processing laggard
Row amplification and fluorimetric analysis；And
Mechanical arm system, the mechanical arm system is for transporting the sample or reagent to the sample sampling device, institute
State kit conveyer, the sample preprocessing device, the nucleic acid-extracting apparatus or the augmentation detection device.
2. nucleic acid extraction as described in claim 1 and amplification fluorescent detection system, which is characterized in that
The kit storage assembly includes multiple for storing the accommodating cavity and cartridge clip shell of kit；Each described appearance
It sets and intracavitary is placed with the cartridge clip shell；Multiple kits overlay in the cartridge clip shell.
3. nucleic acid extraction as claimed in claim 2 and amplification fluorescent detection system, which is characterized in that
The kit conveying mechanism includes gripper assembly, moving track and driving assembly；
The gripper assembly is for taking out the kit from the cartridge clip shell inner clip；Gripper assembly transmission connection in
The driving component, to drive the gripper assembly to move along the moving track.
4. nucleic acid extraction as described in claim 1 and amplification fluorescent detection system, which is characterized in that
The sample preprocessing device includes the turntable and pick-up component for placing kit；The pick-up component is connected to institute
Turntable is stated, and is located at the top of the turntable, the pick-up component, which is able to enter in the kit, to be drawn test solution or stir
It mixes.
5. nucleic acid extraction as claimed in claim 4 and amplification fluorescent detection system, which is characterized in that
The bottom of the turntable is provided with heating component, for heating to the test solution in the kit.
6. nucleic acid extraction as described in claim 1 and amplification fluorescent detection system, which is characterized in that
The augmentation detection device includes detection device, microcosmic chip and chip movement component；The microcosmic chip is for holding
Set multiple samples to be detected；The detection device includes heat circulating system and live signal detection system, the thermal cycle system
System provides suitable temperature for carrying out nucleic acid amplification for the sample in the microcosmic chip；The live signal detection system
It unites for carrying out fluorescence detection to the sample after generation nucleic acid amplification；The chip movement component is described microcosmic for grabbing
Chip and the detection zone that the microcosmic chip is transferred to the detection device.
7. nucleic acid extraction as described in claim 1 and amplification fluorescent detection system, which is characterized in that
The nucleic acid-extracting apparatus includes magnetic separator, nucleic acid extracting reagent storage, heater assembly and liquid relief component；
The magnetic separator is used for test solution and Beads enrichment；The nucleic acid extracting reagent storage is for storing from sample extraction
One or more reagents of nucleic acid；The heater assembly, which is used to provide nucleic acid extraction reaction, is suitble to temperature.
8. such as the described in any item nucleic acid extractions of claim 1-7 and amplification fluorescent detection system, which is characterized in that
The nucleic acid extraction and amplification fluorescent detection system further include the public liquid box of waste liquid；The public liquid box setting of waste liquid
Between the nucleic acid-extracting apparatus and the sample sampling device.
9. nucleic acid extraction as claimed in claim 8 and amplification fluorescent detection system, which is characterized in that
It is provided on each described sample rack multiple for placing the accommodating cavity of specimen bottle；
The sample conveying mechanism includes driving portion and moving guide rail；The driving portion transmission connection is in the moving guide rail；Often
One sample rack is all connected to the moving guide rail.
10. nucleic acid extraction as described in claim 1 and amplification fluorescent detection system, which is characterized in that
The mechanical arm system includes drawing arm and crawl arm；
The position drawn arm and be used to draw test solution and be moved to needs；The crawl arm is for grabbing test solution box and being moved to
The position needed.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811093908.6A CN108865659A (en) | 2018-09-19 | 2018-09-19 | Nucleic acid extraction and amplification fluorescent detection system |
| PCT/CN2019/091421 WO2020057193A1 (en) | 2018-09-19 | 2019-06-14 | Fluorescence detection system for nucleic acid extraction and amplification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811093908.6A CN108865659A (en) | 2018-09-19 | 2018-09-19 | Nucleic acid extraction and amplification fluorescent detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108865659A true CN108865659A (en) | 2018-11-23 |
Family
ID=64324273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811093908.6A Pending CN108865659A (en) | 2018-09-19 | 2018-09-19 | Nucleic acid extraction and amplification fluorescent detection system |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN108865659A (en) |
| WO (1) | WO2020057193A1 (en) |
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Application publication date: 20181123 |