CN117653568A

CN117653568A - Anti-aging composition and application thereof

Info

Publication number: CN117653568A
Application number: CN202311701745.6A
Authority: CN
Inventors: 赵可欣; 罗浩; 张岩; 李季涛
Original assignee: Ailuobi Tianjin Bioengineering Co ltd
Current assignee: Ailuobi Tianjin Bioengineering Co ltd
Priority date: 2023-12-12
Filing date: 2023-12-12
Publication date: 2024-03-08

Abstract

The invention provides an anti-aging composition and application thereof, and belongs to the technical field of skin care products. The anti-aging composition provided by the invention has excellent anti-aging effect through the synergistic effect of the yeast extract, the glycollic acid and the bakuchiol, and can effectively reduce the activity of beta-galactosidase in cells and inhibit cell aging; can also effectively remove DPPH free radical and hydroxyl free radical, and can remarkably improve skin elasticity and reduce wrinkles. According to the invention, the alpine leontopodium herb, the crocus sativus and the cactus are added in the yeast culture process, so that the antioxidation effect of the yeast extract is further improved.

Description

Anti-aging composition and application thereof

Technical Field

The invention relates to the technical field of skin care products, in particular to an anti-aging composition and application thereof.

Background

The skin is an important organ of human body with various physiological functions, and can protect the human body from external environment, prevent internal moisture and useful components from flowing out, regulate body temperature, etc. Since skin is often exposed to various external stimuli, skin is susceptible to wrinkles as compared to other organs. And the facial skin is directly exposed to sunlight, dry environment and pollutants for a longer time, and aging phenomena such as wrinkles and the like occur earlier than the skin which is not exposed to the outside. The most typical changes caused by skin tissue aging are changes in the matrix of the skin (matrix), where human skin fibroblasts (human skin fibroblast) in the dermis layer age, decreasing the ability to produce fibers and matrix, and the overall decrease in the amount of matrix, thinning the skin, decreasing skin elasticity, and forming wrinkles. In addition, skin aging may cause a decrease in skin elasticity, blood circulation disorder, weakening of skin barrier, and the like.

Currently, the most representative anti-aging functional materials used to reduce skin wrinkles and enhance skin elasticity are retinoids, vitamin C, collagen peptides, and the like. Among retinoids, retinoic acid is used as a potent anti-aging therapeutic agent, but has strong irritation to skin, and thus is limitedly used as a therapeutic agent, and retinol, retinol palmitate, etc. having relatively low irritation are widely used as cosmetic materials. However, when retinol and retinol palmitate are irradiated with light, phototoxicity, which causes irritation to the skin, and stability thereof become problems due to denaturation by light. Although vitamin C is also a widely used therapeutic agent for improving wrinkles, it has disadvantages of skin irritation and low stability caused by strong acidity. The collagen peptide can supplement collagen required by skin, maintain skin elasticity, and play a good role in improving skin sagging, but the effect of applying the collagen peptide is not great, and the generated skin wrinkles can be alleviated only by injecting the collagen or the reduced skin matrix components or cells can be supplemented to improve the generated skin wrinkles, but the injection of the collagen is expensive.

Disclosure of Invention

Accordingly, the invention aims to provide an anti-aging composition and application thereof, wherein the anti-aging composition can effectively delay cell aging, improve wrinkles, make skin finer and tighter, and effectively keep skin healthy.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides an anti-aging composition, which comprises the following components in parts by weight: 1 to 10 parts of yeast extract, 0.1 to 1 part of glycollic acid and 0.01 to 0.2 part of bakuchiol.

Preferably, the anti-aging composition comprises the following components in parts by weight: 3 to 8 parts of yeast extract, 0.2 to 0.7 part of glycollic acid and 0.75 to 0.15 part of bakuchiol.

Preferably, the preparation method of the yeast extract comprises the following steps:

(1) Adding a plant preparation into a fermentation medium, and inoculating saccharomycetes for fermentation;

(2) Separating thallus and fermentation liquor, mixing thallus with ethanol solution, extracting for 2-4 h at 25-30 ℃ to obtain ethanol extract;

(3) Concentrating the ethanol extract under reduced pressure at 43-47 ℃, and freeze-drying to obtain the yeast extract.

Preferably, the plant preparation comprises alpine leontopodium herb, crocus sativus and cactus, wherein the weight ratio of the alpine leontopodium herb to the crocus sativus to the cactus is 1 (1-3) (0.5-2); the addition amount of the plant preparation is 10-15 g/L.

Preferably, the temperature of the fermentation is 33-37 ℃, and the time of the fermentation is 48-72 hours.

Preferably, the volume fraction of the ethanol solution is 70-80%, and the weight ratio of the thalli to the ethanol solution is 1:1.5-2.5.

Preferably, the extraction is accompanied by ultrasound, the power of which is 200-300W, and the frequency of which is 20-40 kHZ.

The invention also provides application of the anti-aging composition in preparing skin care products.

Preferably, the weight percentage of the anti-aging composition is 0.5-5%.

Preferably, the skin care product comprises moisturizing water, toner, astringent, toner, emulsion, face cream, eye cream, facial mask, essence, essential oil, barrier cream or sun cream.

By adopting the technical scheme, the invention has the following beneficial effects: the anti-aging composition provided by the invention has excellent anti-aging effect through the synergistic effect of the yeast extract, the glycollic acid and the bakuchiol, and can effectively reduce the activity of beta-galactosidase in cells and inhibit cell aging; can also effectively remove DPPH free radical and hydroxyl free radical, and can remarkably improve skin elasticity and reduce wrinkles. According to the invention, the alpine leontopodium herb, the crocus sativus and the cactus are added in the yeast culture process, so that the antioxidation effect of the yeast extract is further improved.

Detailed Description

In the anti-aging composition, the weight part of the yeast extract is 1 to 10 parts, more preferably 3 to 8 parts, still more preferably 5 parts;

the weight part of the glycollic acid is 0.1 to 1 part, more preferably 0.2 to 0.7 part, and still more preferably 0.3 part;

the weight part of the bakuchiol is 0.01-0.2 part, more preferably 0.075-0.15 part, and still more preferably 0.1 part.

Glycolic acid, which includes fruit acid or alpha-hydroxy acid, is a natural product isolated from sucrose; citric acid from oranges and other citrus fruits can also be classified as glycolic acid. Glycolic acid can remove dead skin cells and excess grease and stimulate fibroblasts in the dermis to produce more collagen.

The bakuchiol is a monoterpene compound separated from the bakuchiol of leguminous plants, can control oil and resist acne, inhibit bacteria and inflammation, improve pigmentation, and has the effects of resisting oxidation and aging, reducing fine lines and wrinkles, stimulating collagen regeneration, enhancing skin elasticity and compactness, preventing ultraviolet rays from damaging skin, and delaying apoptosis of cells.

The yeast extract is prepared by breaking cell wall of yeast, extracting protein, nucleic acid, vitamins, etc., and performing biological enzymolysis to obtain natural active ingredients rich in small molecular amino acids, peptides, nucleotides, vitamins, etc. Wherein, the amino acid content is more than 30%, the total protein is more than 50%, and the nucleotide is more than 10%, and the product has the functions of moisturizing, diminishing inflammation, resisting oxidation and aging.

In the present invention, the preparation method of the yeast extract comprises the following steps:

In the invention, a plant preparation is added into a fermentation medium, and yeast is inoculated for fermentation; the fermentation medium is preferably LB fermentation medium, and the yeast is activated before inoculation, so that the invention has no special limit on the activation of the yeast and can achieve the purpose of activating the thallus. After activating the saccharomycetes, inoculating the saccharomycetes for fermentation, wherein the inoculation amount of the saccharomycetes is 5-12%, more preferably 7-10%, and even more preferably 8%; the yeast may be Saccharomyces cerevisiae, cryptococcus, candida, but is not limited thereto.

In the present invention, the plant preparation includes alpine leontopodium herb, crocus sativus and cactus fruit; the radix et caulis Opuntiae Dillenii, stigma croci, and radix et caulis Opuntiae Dillenii are dried radix et rhizoma Rhei-grass, stigma croci, and radix et caulis Opuntiae Dillenii with water content below 12%; the alpine leontopodium herb adopts flowers or leaves of the alpine leontopodium herb, the crocus sativus adopts crocus sativus petals, and the cactus fruit is a cactus fruit. The weight ratio of the alpine leontopodium herb, the crocus sativus and the cactus is 1 (1-3) (0.5-2), more preferably 1 (1.5-2.5) (1-1.5), and more preferably 1:2:8. According to the invention, flowers or leaves of alpine leontopodium herb, crocus sativus and cactus are ground into powder, and the powder is added into a fermentation medium, wherein the mesh number of the powder is 80-120 meshes, more preferably 90-110 meshes, and still more preferably 100 meshes. The alpine leontopodium has the functions of resisting oxidation, scavenging free radicals, strengthening skin barrier, repairing and relieving, whitening and nourishing skin; the crocus sativus contains rich antioxidant substances, so that the damage of free radicals to skin cells can be neutralized, and the skin aging can be prevented; the radix et caulis Opuntiae Dillenii can be used for preventing and treating acne caused by high androgen, and promoting skin growth factor. The addition of the plant preparation further enhances the antioxidant activity of the strain.

In the present invention, the fermentation temperature is 33 to 37 ℃, more preferably 34 to 36 ℃, still more preferably 35 ℃; the fermentation time is 48 to 72 hours, more preferably 55 to 65 hours, still more preferably 60 hours; the fermentation is carried out under stirring conditions, and the stirring speed is 100 to 300rpm, more preferably 150 to 250rpm, still more preferably 200rpm.

After the fermentation is completed, the fermentation product is separated into thalli and fermentation liquor by centrifugation, wherein the rotation speed of the centrifugation is 6000-8000 rpm, more preferably 6500-7500 rpm, and even more preferably 7000rpm; the centrifugation time is 8 to 12 minutes, more preferably 9 to 11 minutes, still more preferably 10 minutes; obtaining saccharomycetes after centrifugation, mixing the saccharomycetes with an ethanol solution for extraction, wherein the volume fraction of the ethanol solution is 70-80%, more preferably 73-78%, still more preferably 75%; the weight ratio of the saccharomycetes to the ethanol solution is 1:1.5-2.5, more preferably 1:1.8-2.2, and still more preferably 1:2; the extraction temperature is 25-30 ℃, more preferably 27-29 ℃, and still more preferably 28 ℃, and the temperature range can protect active ingredients in the strain lysate or culture solution from thermal denaturation; the extraction time is 2 to 4 hours, more preferably 2.5 to 3.5 hours, still more preferably 3 hours; the extraction is accompanied by ultrasound, the power of the ultrasound is 200-300W, more preferably 230-270W, and even more preferably 250W; the frequency of the ultrasonic wave is 20-40 kHZ, more preferably 25-35 kHZ, more preferably 30kHZ, and the purpose of the ultrasonic wave is to generate cavitation effect in the liquid by using strong ultrasonic wave, promote the breaking of bacteria and improve the extraction efficiency. After the extraction is completed, an ethanol extract is obtained.

After the ethanol extract is obtained, the ethanol extract is subjected to reduced pressure concentration, wherein the temperature of the reduced pressure concentration is 43-47 ℃, more preferably 44-46 ℃, and still more preferably 45 ℃; the vacuum degree of the reduced pressure concentration is-0.1 to-0.08 MPa, more preferably-0.095 to-0.085 MPa, and still more preferably-0.09 MPa; the reduced pressure concentration was performed under water bath conditions. Concentrating the ethanol extract to an extract with the density of 1.3-1.7 g/mL, more preferably 1.4-1.6 g/mL, more preferably 1.5g/mL, and freeze-drying after the concentration is finished, wherein the freeze-drying temperature is-35 to-15 ℃, more preferably-30 to-20 ℃, more preferably-25 ℃; the vacuum degree of vacuum freeze-drying is 20 to 40Pa, more preferably 25 to 35Pa, still more preferably 30Pa, to obtain yeast extract.

The invention also provides application of the anti-aging composition in preparing skin care products, wherein the weight percentage of the anti-aging composition is 0.5-5%, more preferably 1-4%, and still more preferably 3%. The skin care product comprises moisturizing water, toner, astringent, skin softening water, lotion, cream, eye cream, facial mask, essence, essential oil, barrier cream or sun cream.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

Unless otherwise specified, reagents and materials used in the following examples are all commercially available; bakuchiol is purchased from Shanghai Union industries, inc. or self-produced, and glycolic acid is purchased from Hebei Chengxi, inc. or self-produced; the saccharomycete in the embodiment is selected from saccharomycete CCTCCWY2008323 and is purchased from a China center for type culture Collection.

Example 1

Inoculating the saccharomycetes CCTCC WY2008323 into an LB liquid medium, and standing and culturing for 24 hours at 35 ℃ to obtain fully activated saccharomycetes liquid. Inoculating yeast liquid into LB liquid fermentation medium according to 5% of inoculation amount, fermenting at 33 ℃ for 48 hours at 100rpm, adding 10g/L of plant preparation into the LB liquid fermentation medium, mixing dry alpine leonurus leaf, crocus sativus petal and cactus fruit according to the weight ratio of 1:1:0.5, and separating yeast thallus and fermentation liquor at 6000rpm after fermentation.

Mixing saccharomycetes and 70% ethanol solution according to a weight ratio of 1:1.5, performing ultrasonic extraction for 2 hours at 25 ℃, wherein the ultrasonic power is 200W, the frequency is 20kHZ, and obtaining ethanol extract after the extraction is completed. Concentrating the ethanol extract under reduced pressure at-35deg.C and-0.1 MPa, concentrating the ethanol extract to obtain extract with density of 1.3g/mL, and lyophilizing at-35deg.C and 20Pa to obtain yeast extract.

In the anti-aging composition of this example, the yeast extract, glycolic acid and bakuchiol prepared as described above were mixed at a mass ratio of 1:0.1:0.01, and specifically, 1g of the yeast extract, 0.1g of glycolic acid and 0.01g of bakuchiol were mixed.

Example 2

Inoculating the saccharomycetes CCTCC WY2008323 into an LB liquid medium, and standing and culturing for 24 hours at 35 ℃ to obtain fully activated saccharomycetes liquid. Inoculating the saccharomycete liquid into an LB liquid fermentation medium according to the inoculation amount of 8%, fermenting at 35 ℃ for 60 hours at 200rpm, adding 12g/L of plant preparation into the LB liquid fermentation medium, mixing dry alpine leonia leaves, crocus sativus petals and cactus fruits according to the weight ratio of 1:2:1, and separating saccharomycete bodies and fermentation liquor at 7000rpm after the fermentation is finished.

Mixing saccharomycetes and 75% ethanol solution according to a weight ratio of 1:2, carrying out ultrasonic extraction for 3 hours at 28 ℃, wherein the ultrasonic power is 280W, the frequency is 40kHZ, and obtaining ethanol extract after the extraction is completed. Concentrating the ethanol extract under reduced pressure at 45 deg.C and-0.09 MPa, concentrating the ethanol extract to obtain extract with density of 1.5g/mL, and freeze drying at-25deg.C and 30Pa to obtain yeast extract.

In the anti-aging composition of this example, the yeast extract, glycolic acid and bakuchiol prepared as described above were mixed at a mass ratio of 5:0.3:0.1, and specifically, 5g of the yeast extract, 0.3g of glycolic acid and 0.1g of bakuchiol were mixed.

Example 3

Inoculating the saccharomycetes CCTCC WY2008323 into an LB liquid medium, and standing and culturing for 24 hours at 35 ℃ to obtain fully activated saccharomycetes liquid. Inoculating the saccharomycete liquid into an LB liquid fermentation medium according to 12% of inoculation amount, fermenting at 37 ℃ for 72 hours at 300rpm, adding 15g/L of plant preparation into the LB liquid fermentation medium, mixing dry alpine leonia leaves, crocus sativus petals and cactus fruits according to the weight ratio of 1:3:2, and separating saccharomycete bodies and fermentation liquor at 8000rpm after fermentation.

Mixing saccharomycetes and 80% ethanol solution according to a weight ratio of 1:2.5, carrying out ultrasonic extraction for 4 hours at 30 ℃, wherein the ultrasonic power is 400W, the frequency is 30kHZ, and obtaining ethanol extract after the extraction is completed. Concentrating the ethanol extract under reduced pressure at 47 deg.C and-0.1 MPa, concentrating the ethanol extract to obtain extract with density of 1.7g/mL, and freeze drying at-15deg.C and 40Pa to obtain yeast extract.

In the anti-aging composition of this example, the yeast extract, glycolic acid and bakuchiol prepared as described above were mixed at a mass ratio of 10:1:0.2, and specifically, 10g of the yeast extract, 1g of glycolic acid and 0.2g of bakuchiol were mixed.

Experimental example 1 Effect of different anti-aging substances on inhibition ratio of beta-galactosidase in zebra fish

The experimental example prepared various anti-aging substances with different components and contents, and as shown in table 1, the preparation method of the yeast extracts in comparative examples 1, 4, 5, 7 and 8 is the same as that of example 1; the yeast extract of comparative example 9 is different from that of comparative example 1 in that no alpine leontopodium herb leaves, crocus sativus petals and cactus were added during the yeast culture.

TABLE 1 content of anti-aging substances of different Components

Group of	Anti-aging substance component and its content
		Comparative example 1	Yeast extract 3g
Comparative example 2	Glycolic acid 0.3g
		Comparative example 3	Bakuchiol 0.1g
Comparative example 4	Yeast extract 3 g+glycolic acid 0.3g
		Comparative example 5	Yeast extract 3 g+bakuchiol 0.1g
Comparative example 6	Glycolic acid 0.3g and bakuchiol 0.1g
		Comparative example 7	Yeast extract 1 g+glycolic acid 0.3 g+bakuchiol 0.3g
Comparative example 8	Yeast extract 0.5 g+glycolic acid 0.3 g+bakuchiol 0.1g
		Comparative example 9	Yeast extract 3g
Example 1	Yeast extract 1g+0.1g glycolic acid+0.01 g+bakuchiol 0.01g
		Example 2	Yeast extract 3 g+glycolic acid 0.3 g+bakuchiol0.1g
Example 3	Yeast extract 10g+0.2g glycolic acid+0.2 g bakuchiol 0.2g

Zebra fish is a spinal model organism, which is highly homologous with human beings in gene and protein levels and is quite similar to human beings in terms of development process, organ structure, physiological functions and the like; meanwhile, the aging process of the zebra fish is more similar to that of the human being compared with that of mice and the human being, and based on the characteristics, the anti-aging research is carried out by taking the AB-series zebra fish as an experimental animal, and the AB-series zebra fish is purchased from the aquatic organism research institute of China academy of sciences.

Female and male zebra fish of 6 months of age are separately cultured at 28.5 ℃ under the condition of 14h illumination/10 h darkness, and 2 times of artemia are fed at 10:00 (am) and 4:00 (pm) each day. And (3) the day before the embryo is obtained by mating, putting the healthy mature zebra fish into a mating cylinder according to the proportion of male to female of 1:2, placing a partition board in the middle, and placing the zebra fish in a dark environment. The baffle is drawn out before the next day of lighting, and the light stimulates the female fish to ovulate. Adult fish are fished out half an hour after ovulation, fertilized eggs are collected and sterilized. The fertilized eggs are placed in a constant temperature incubator at 28.5 ℃ and are cultured for 14 hours under illumination/10 hours in the dark period.

AB-line zebra fish 8h (8 hpf) after fertilization were randomly divided into 13 groups of 15 embryos per dish:

the first group was untreated and served as a control group;

the second group is H ₂ O ₂ Model group, H with final concentration of 0.5mmol/L is added in 72H after hatching of zebra fish ₂ O ₂ A solution;

the first group added H with the final concentration of 0.5mmol/L within 72H after the hatching of the zebra fish ₂ O ₂ The content of the anti-aging substances in the solution and the anti-aging substances in the comparative example 1 in the zebra fish culture solution is 10 mug/mL, 25 mug/mL, 50 mug/mL and 100 mug/mL respectively;

the second group adds H with the final concentration of 0.5mmol/L in 72H after the hatching of the zebra fish ₂ O ₂ The content of the anti-aging substances in the solution and the anti-aging substances in the comparative example 2 in the zebra fish culture solution is respectively 10 mug/mL, 25 mug/mL, 50 mug/mL and 100 mug/mL;

the third group to the eleventh group are respectively added with H with the final concentration of 0.5mmol/L within 72 hours after the hatching of the zebra fish ₂ O ₂ The solutions and the anti-aging substances of comparative examples 3 to 9 and examples 1 to 3 were such that the content of the anti-aging substances in the zebra fish culture broth was 10. Mu.g/mL, 25. Mu.g/mL, 50. Mu.g/mL, 100. Mu.g/mL, respectively; after each of the above groups was continuously treated in a constant temperature incubator at 28.5℃for 72 hours, embryos were washed with zebra fish culture water, and then the beta-galactosidase activity in zebra fish bodies was detected using a beta-galactosidase activity detection kit (Beijing Box Biotechnology Co., ltd.) according to the instructions, and the inhibition ratio of the beta-galactosidase activity was calculated using the following formula, and the results are shown in Table 2.

Wherein A1 is the activity of beta-galactosidase in zebra fish bodies of a control group;

a2 is the activity of beta-galactosidase in the zebra fish body of the model group;

a3 is the activity of beta-galactosidase in the zebra fish body added with anti-aging substances in the culture solution;

TABLE 2 inhibition of beta-galactosidase Activity by different groups of anti-aging substances

Beta-galactosidase (SA-beta-Gal) is a lysosomal hydrolase that is expressed only in senescent cells to catalyze the hydrolysis of beta-galactoside, the increase in activity being a significant feature of cell aging. As is clear from Table 2, the yeast extract, glycolic acid and bakuchiol all had an inhibitory effect on beta-galactosidase in the concentration range of 10 to 100. Mu.g/mL, and the inhibitory effect was enhanced with increasing concentration. The yeast extract of comparative example 9 has a lower beta-galactosidase activity than comparative example 1, demonstrating that the addition of alpine leontopodium herb, crocus sativus and opuntia ficus tikoua during the yeast culture can increase the inhibition of beta-galactosidase by the yeast extract.

As is clear from comparative examples 1 to 3 and comparative examples 7 to 8, the inhibition ratio of the anti-aging substance consisting of yeast extract, glycolic acid, bakuchiol to the beta-galactosidase activity was higher than the sum of the inhibition ratios of the individual actions of yeast extract, glycolic acid, bakuchiol. As is clear from comparative examples 4 to 6 and comparative examples 7 to 8, the effect of inhibiting beta-galactosidase was increased by combining the yeast extract, glycolic acid and bakuchiol in two pairs, but was lower than the effect of combining the three. It can be seen that the combination of the yeast extract, the glycollic acid and the bakuchiol can synergistically inhibit the increase of beta-galactosidase and inhibit cell aging. As is clear from comparative examples 7 to 8 and examples 1 to 3, although the anti-aging compositions of comparative examples 7 to 8 comprise three components of yeast extract, glycolic acid and bakuchiol, the ratio of the three components is not within the scope of the present invention, and the inhibition effect on beta-galactosidase is not as good as that of examples 1 to 3 of the present invention.

In conclusion, the anti-aging composition prepared by compounding the yeast extract, the glycollic acid and the bakuchiol has an inhibition effect on beta-galactosidase, the drug effect is superior to the effect of single use of each component or the superposition of the two components by matching, the anti-aging composition has a remarkable synergistic effect, and the anti-aging effect is optimal when the weight ratio of the yeast extract to the glycollic acid to the bakuchiol is within the range of (1-10): (0.1-1): (0.01-0.2).

Experimental example 2 scavenging test of free radical by different anti-aging substances

The experimental samples used in this experimental example were prepared in the same manner as in experimental example 1, and the anti-aging substances of examples 1 to 3 and comparative examples 1 to 9 were tested for radical scavenging ability

Test sample: examples 1 to 3 and comparative examples 1 to 8 as antiaging substances

(1) Determination of the scavenging Capacity of 1, 1-diphenyl-2-trinitrobenzene liver (DPPH) free radical

Reagent: 1, 1-diphenyl-2-trinitroskin and absolute ethyl alcohol.

Preparing a test sample: 10mg of the anti-aging substances of examples 1 to 3 and comparative examples 1 to 9 were taken out, respectively, and dissolved with absolute ethanol to a volume of 200mL for use.

The testing method comprises the following steps: accurately sucking 2.0mL of the prepared test sample and 2.0mL of the LDPPH solution, placing the test sample and the solution into a test tube, vibrating and uniformly mixing, and placing the test sample and the solution into an incubator to be kept at the constant temperature of 37 ℃ for 1h. Accurately sucking 2.0mL of prepared test sample and 2.0mL of absolute ethyl alcohol by taking absolute ethyl alcohol as a blank control, placing the test sample and the 2.0mL of absolute ethyl alcohol into a test tube, vibrating and uniformly mixing, and then placing the test sample and the test tube into a constant temperature box for constant temperature 1h at 37 ℃; accurately sucking 2.0mL of the prepared DPPH solution and 2.0mL of absolute ethyl alcohol, placing into a test tube, shaking and mixing uniformly, and then placing into a constant temperature box for constant temperature 1h at 37 ℃. Each sample was tested for absorbance at 517 nm.

Clearance (%) = [1- (a) _x -A _j )/A _c ]X 100%; wherein A is _c Absorbance of 2.0mL DPPH solution and 2.0mL absolute ethanol mixed solution; a is that _x Absorbance of 2.0mL test sample and 2.0mL DPPH solution mixture; a is that _j The absorbance was 2.0mL of the test sample and 2.0mL of the absolute ethanol mixed solution. Three replicates were tested for each set of samples and averaged, the results are shown in table 3.

(2) Determination of the scavenging Capacity for hydroxyl radicals (. OH)

Reagent: 2mmol/L FeSO ₄ Solution, 1mmol/LH ₂ O ₂ Solution, 6mmol/L salicylic acid solution.

10mg of the anti-aging substances of examples 1 to 3 and comparative examples 1 to 8 were taken respectively, dissolved with water and fixed to 200mL.

And (3) preparation of a reagent: 3mL of FeSO with a concentration of 2mmol/L are added into a test tube in sequence ₄ Solution, 3mL H with concentration of 1mmol/L ₂ O ₂ The solution is shaken up, then 3mL of salicylic acid solution with the concentration of 6mmol/L is added, and the solution is shaken up; heating in 37deg.C water bath for 15min, and taking out.

The testing method comprises the following steps: measuring the absorbance of the formulated reagent; adding the prepared test sample, shaking uniformly, heating in water bath for 15min, taking out, and measuring absorbance; water was used as a blank.

Clearance (%) = [ a _n -A _m -(A _n -A _k )]/A _k X 100%; wherein An is the absorbance of the formulated reagent, A _m To add absorbance after test sample has been cleared of OH, A _k Absorbance was used as a blank. Three replicates were tested for each set of samples and averaged, the results are shown in table 3.

TABLE 3 scavenging of free radicals by different groups of anti-aging substances

As shown in Table 3, the yeast extract, the glycollic acid and the bakuchiol have the effect of scavenging DPPH free radicals and hydroxyl free radicals, but the scavenging rate of the combination of the yeast extract, the glycollic acid and the bakuchiol on the DPPH free radicals and the hydroxyl free radicals is superior to the superposition of the independent use of each component or the combination of the two components, which indicates that the yeast extract, the glycollic acid and the bakuchiol are compounded, and the components are synergistic mutually, so that the composition has better scavenging efficiency on the free radicals and further has more excellent anti-aging effect. Meanwhile, as is clear from comparative examples 1 and 9, the addition of alpine leontopodium herb, crocus sativus and cactus fruit during the yeast culture can improve the radical scavenging rate of the yeast extract.

Experimental example 3 measurement of skin improvement Effect

Comparative example 10

The yeast extract of example 1 was replaced with the yeast extract prepared in comparative example 9, and the contents of the yeast extract, glycolic acid and bakuchiol were the same as in example 1.

The anti-aging compositions of examples 1 to 3 and comparative examples 7, 8, and 10 were prepared into emulsions according to the following system:

the emulsion comprises the following components in percentage by weight: 1% of an anti-aging composition, 15% of dipropylene glycol, 7.5% of octyl dodecanol myristate, 3% of carbomer, 10% of glycerol, 6% of allantoin, 2.5% of xanthan gum, 3% of beeswax, 5% of glycerol stearate and the balance of water.

The test was performed by randomly extracting 60 skin-healthy volunteers, one group of 10 persons, each group being a unit, corresponding to the emulsions of comparative examples 7 to 9 of test examples 1 to 3, respectively, and the test results were all the average value of each group.

Inclusion volunteer criteria were: women aged 30-50 years; those who have not used hormonal drugs and immunosuppressants for nearly 30 days; the tested part does not participate in other clinical trial persons within 30 days; during the test, it was agreed that no cosmetics, drugs and health products having an influence on the results were used.

Exclusion criteria: those with open wounds or erosive surfaces; highly allergic constitution; pregnancy, lactation or pregnancy-preparing person during testing; has severe barycenter, liver and kidney function injury and severe hypoimmunity; patients with mental diseases and severe endocrine diseases and patients with oral contraceptives; clinical testers or other testers taking medicines within 30 days, or those who have medicines with systematic effects on test results within nearly 1 week; within 2 weeks there were cosmetic products that could have an effect on the test results for oral and topical use.

Abort and exit criteria: smearing the tested sample or performing a return visit record according to the requirement in the test period; during use, suffering from serious diseases or suffering from serious adverse reactions.

The testing method comprises the following steps: after the volunteers cleansing the face in the morning and evening each day, the skin elasticity and wrinkles were measured before and after the experiment, respectively, using the above emulsion on the whole face for 30 consecutive days.

Skin elasticity was measured by using a skin elasticity tester Cutometer dual MPA from CK company, germany, and wrinkles were detected by using a visual skin detector and automatically analyzed to calculate the skin elasticity improvement rate and the wrinkle removal rate, and the test results are shown in table 4.

TABLE 4 improvement of skin by emulsions of different groups

As can be seen from Table 4, the emulsion prepared from the anti-aging composition of the present invention has the effect of remarkably improving skin elasticity and reducing wrinkles, and has the best effect of improving skin when the weight ratio of the yeast extract, the glycolic acid and the bakuchiol is (1-10): 0.1-1): 0.01-0.2; the emulsion prepared in comparative example 10 had a lower effect on improving skin than examples 1 to 3 and comparative examples 7 and 8, indicating that the addition of alpine leontopodium herb, crocus sativus and cactus during yeast culture can further improve skin elasticity and reduce wrinkles.

From the above examples, the present invention provides an anti-aging composition and its use. The anti-aging composition provided by the invention can effectively reduce the activity of beta-galactosidase, remove free radicals, improve skin elasticity and reduce wrinkles.

The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims

1. An anti-aging composition is characterized by comprising the following components in parts by weight: 1 to 10 parts of yeast extract, 0.1 to 1 part of glycollic acid and 0.01 to 0.2 part of bakuchiol.

2. The aging composition according to claim 1, comprising the following components in parts by weight: 3 to 8 parts of yeast extract, 0.2 to 0.7 part of glycollic acid and 0.75 to 0.15 part of bakuchiol.

3. The anti-aging composition according to claim 1 or 2, wherein the method for preparing the yeast extract comprises the steps of:

4. The anti-aging composition according to claim 3, wherein the plant preparation comprises alpine leontopodium herb, crocus sativus and opuntia ficus-indica in a weight ratio of 1 (1-3): 0.5-2; the addition amount of the plant preparation is 10-15 g/L.

5. An anti-ageing composition according to claim 3, wherein the fermentation is carried out at a temperature of 33-37 ℃ for a period of 48-72 hours.

6. The anti-aging composition according to claim 3, wherein the volume fraction of the ethanol solution is 70-80%, and the weight ratio of the bacterial cells to the ethanol solution is 1:1.5-2.5.

7. The anti-aging composition according to claim 3, wherein the extraction is accompanied by ultrasound, the power of which is 200-300W, and the frequency of which is 20-40 kHZ.

8. Use of an anti-aging composition according to any one of claims 1 to 7 in the preparation of a skin care product.

9. The use according to claim 8, wherein the anti-aging composition is present in the skin care product in an amount of 0.5 to 5% by mass.

10. The use according to claim 8, wherein the skin care product comprises water, lotion, cream, essence or mask products.