CN117586067A - Amino acid chelated organic fertilizer and production process thereof - Google Patents

Abstract

The invention discloses an amino acid chelated organic fertilizer and its production process, which belongs to the technical field of amino acid processing. The amino acid chelated organic fertilizer includes the following parts by weight of raw materials: 42-50 parts of compound amino acid chelated calcium and 16-22 parts of film-forming liquid. , 12-20 parts of corn straw, 12-15 parts of wheat straw, 8-12 parts of soybean meal, 0.3-0.5 parts of fermentation bacteria, and 0.2-0.4 parts of cellulase. The invention discloses an amino acid chelated organic fertilizer. The raw materials include complex amino acid chelated calcium, film-forming liquid, corn straw, wheat straw, soybean meal, fermentation bacteria and cellulase. The amino acid organic fertilizer prepared by the invention can improve soil. Crops can directly absorb and utilize amino acids. The amino acid chelated organic fertilizer prepared by the invention not only has a good growth-promoting effect on crop growth, but also can make full use of biological resources in the production of amino acids.

Description

An amino acid chelated organic fertilizer and its production process

Technical field

The invention belongs to the technical field of amino acid processing, and specifically relates to an amino acid chelated organic fertilizer and its production process.

Background technique

Fertilizers can be divided into inorganic fertilizers (chemical fertilizers) and organic fertilizers. Inorganic fertilizers can provide the nutrients needed for crop growth in a timely and rapid manner, but excessive application can lead to soil hardening and quality degradation, further causing environmental problems. Relatively speaking, organic fertilizers have less impact on the soil environment. The nutrients and mineral elements contained in organic fertilizers play an important role in increasing soil enzyme activity, the number of microorganisms, and soil nutrients. However, organic fertilizers have limited nutrient content and slow onset of fertilizer effect. . Therefore, in recent years, many manufacturers have devoted themselves to the development of improved organic fertilizers, among which amino acid organic fertilizers are one of the research hotspots.

There are many types of amino acids and different physiological functions of different amino acids are different. Most amino acids have the function of growth regulators and can promote photosynthesis and respiration of plants. At the same time, due to the functional groups such as carboxyl and amino groups contained in amino acids, they have a certain chelating ability. The carboxyl groups of amino acids and trace elements are covalently combined through one or more coordination bonds to form a stable complex.

In the current agricultural production process, the application of trace elements in crop production mostly adopts the method of foliar spraying. Some trace elements are difficult to diffuse into the plant through the leaf surface due to their relatively large molecular weight. Therefore, the use of amino acids and trace elements Based on the chelating ability, an amino acid chelated organic fertilizer was developed, in which the amino acid trace element complex uses amino acids as carriers to participate in the synthesis of protein in plants and can be directly absorbed and utilized by plants. Compared with inorganic fertilizers, it helps It improves the effective absorption of trace elements and at the same time helps increase the absorption and transport rate of trace elements by plants. Therefore, it is very necessary to develop an amino acid chelated organic fertilizer.

Contents of the invention

The present invention aims to utilize biological resources (animal feathers and hair, etc.) to develop amino acid chelated organic fertilizers, and aims to improve the absorption efficiency of trace elements by plants, thereby improving the utilization rate of fertilizers. One purpose of the present invention is to provide an amino acid Chelated organic fertilizer, another object of the present invention is to provide the production process of the above-mentioned amino acid chelated organic fertilizer.

The object of the present invention can be achieved through the following technical solutions:

An amino acid chelated organic fertilizer includes the following raw materials in parts by weight:

Further, the complex amino acid chelated calcium is prepared by the following steps:

Stir and add shell powder to 2mol/L dilute hydrochloric acid solution, stir until the solid components are completely dissolved to obtain a calcium chloride solution, then add complex amino acids to the calcium chloride solution, stir and mix for 3-4 hours, and then add to the system Adjust the pH to 8.9-9.2 with an NH ₃ -NHCl ₃ buffer solution with a pH of 10.4. After the pH adjustment is completed, a mixed solution is obtained. The mixed solution is heated to 65-70°C, stirred and chelated for 1.2-1.5 hours. After the chelation reaction is completed , to obtain the chelate solution, centrifuge the chelate solution at high speed for 10-12 minutes. After the centrifugation is completed, take the supernatant and concentrate it under reduced pressure, then dialyze and purify with absolute ethanol for 2-3 days, centrifuge, and take the precipitate to obtain the complex amino acid. Chelated calcium.

Further, the dosage ratio of the 2 mol/L dilute hydrochloric acid solution, shell powder, and complex amino acid is 190-200 mL: 85-90 g: 35.4-36.0 g.

Further, the centrifugation speed of the high-speed centrifugation is 3500-4000r/min.

Further, the shell powder is prepared by the following steps:

Rinse the shell surface with clean water, soak the treated shells in 2mol/L dilute hydrochloric acid for 30-40 minutes to dissolve the chitosan and chitin on the shell surface, take out the shells, rinse them with distilled water for 8-10 minutes, and then place them in Dry in an oven at 80-100°C until constant weight, crush and pass through a 100-mesh sieve to obtain shell powder.

Further, the composite amino acid is prepared by the following steps:

Step S1: Stir and add washed and dried animal hair to 6-8 mol/L hydrochloric acid solution, and place it at 90-95°C for hydrolysis for 4-5 hours. After the hydrolysis is completed, continue to add 6-8 mol/L of hydrochloric acid solution. hydrochloric acid solution until the pH of the system is 1.4-1.6 to obtain a hair hydrolyzate;

Step S2: Stir and add 1/3 of the total amount of biphenyl-4-sulfonic acid to the hair hydrolyzate, mix and stir for 3-4 hours to obtain a first mixed liquid, cool the first mixed liquid to 8-10°C, and let it stand for 24 hours. Filter to obtain the tyrosine precipitate and the first mother liquor, and then use 201×7 anion exchange resin to separate and purify the tyrosine precipitate to obtain amino acid A; then stir and add the remaining biphenyl-4 to the first mother liquor. -Sulfonic acid, mix and stir for 2-3h to obtain a second mixed liquid. Cool the second mixed liquid to 0-4°C, let it stand for 36 hours and then filter it to obtain the leucine precipitate and the second mother liquid, and then use the 201×7 type anion The exchange resin is used to separate and purify the leucine precipitate to obtain amino acid B; finally, stir and add 6 mol/L ammonia water to the second mother liquor to adjust the pH to 4.9-5.1, let it stand for 24 hours and then filter, to obtain the solid component. The solid component Dry to constant weight at 40-45°C to obtain amino acid C. Mix amino acid A, amino acid B and amino acid C according to a mass ratio of 3.5-4.0:1.2-1.5:1.2-1.5 to obtain a compound amino acid.

Further, the dosage ratio of the 6-8 mol/L hydrochloric acid solution and the washed and dried animal hair described in step S1 is 2L:1.5-1.8kg.

Further, the dosage ratio of the hair hydrolyzate and biphenyl-4-sulfonic acid described in step S2 is 1L:180-210g.

Further, the film-forming liquid is prepared by the following steps:

Stir and add polyvinyl alcohol, humic acid, polyethylene grafted maleic anhydride and Tween-60 to the deionized water, stir and mix for 30-50 minutes, and obtain a film-forming liquid.

Further, the usage ratio of the deionized water, polyvinyl alcohol, humic acid, polyethylene grafted maleic anhydride and Tween-60 is 80-90mL:35-42g:5-7g:0.5-1.0g: 0.1-0.2mL.

Further, the production process of the amino acid chelated organic fertilizer includes the following steps:

Weigh each raw material according to the weight portion, wash and dry the corn straw and wheat straw, crush and mix them with a kneading machine, and then steam soften them at 110-120°C for 2-3 hours to obtain softened straw material. The softened straw material Transfer the soybean meal and soybean meal to the fermentation tank, add fermentation strains and cellulase, raise the temperature to 45-48°C, adjust the pH to 6.2-6.5, ferment at a constant temperature for 36-48 hours, filter after the fermentation is completed, and place the solid component at 100- Sterilize at 120°C for 15-20 minutes to obtain the fermentation component, stir and mix the fermentation component and complex amino acid chelated calcium for 30 minutes to obtain the amino acid chelated organic fertilizer core, and then place the amino acid chelated organic fertilizer core in the spray transfer In the drum granulator, the film-forming liquid is coated on the surface of the amino acid chelated organic fertilizer fertilizer core, and after drying, the amino acid chelated organic fertilizer is obtained.

Further, the fermentation strain is Geotrichum candidum yeast.

Beneficial effects of the present invention:

The invention discloses an amino acid chelated organic fertilizer. The raw materials include complex amino acid chelated calcium, film-forming liquid, corn straw, wheat straw, soybean meal, fermentation bacteria and cellulase. The amino acid organic fertilizer prepared by the invention can improve soil. , crops can directly absorb and utilize amino acids. The prepared amino acid chelated organic fertilizer not only has a good growth-promoting effect on crop growth, but also the production of amino acids can make full use of biological resources.

At the same time, the outer layer of the amino acid chelated organic fertilizer prepared by the present invention is covered with a coating. On the one hand, it prolongs the action time of the fertilizer. On the other hand, the coating contains humic acid, which can promote the soil's response to amino acids, trace elements and The absorption of organic matter improves the fertility of amino acid chelated organic fertilizer.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

Example 1

Prepare complex amino acids:

Hair hydrolysis: Stir and add 1.5kg of washed and dried animal hair into 2L of 6mol/L hydrochloric acid solution, and place it at 90°C for hydrolysis for 4 hours. After the hydrolysis is completed, continue to add 6mol/L hydrochloric acid solution. Until the pH of the system reaches 1.4, a hair hydrolyzate is obtained.

Amino acid extraction: Stir and add 60g of biphenyl-4-sulfonic acid (98%, purchased from Shanghai McLean Biochemical Technology Co., Ltd.) to 1L of hair hydrolyzate, mix and stir for 3 hours to obtain the first mixed liquid, and cool the first mixed liquid. to 8°C, let it stand for 24 hours and then filter to obtain the tyrosine precipitate and the first mother liquor. Then use 201×7 anion exchange resin (purchased from Tianjin Yinghai Chemical Group Co., Ltd.) to separate and purify the tyrosine precipitate. Obtain amino acid A (tyrosine); then stir and add 120g of biphenyl-4-sulfonic acid to the first mother liquid, mix and stir for 2 hours to obtain a second mixed liquid, cool the second mixed liquid to 0°C, and let it stand for 36 hours before filtering. , obtain the leucine precipitate and the second mother liquor, and then use 201×7 type anion exchange resin (purchased from Tianjin Yinghai Chemical Group Co., Ltd.) to separate and purify the leucine precipitate to obtain amino acid B (leucine); Finally, stir and add 6 mol/L ammonia to the second mother liquor to adjust the pH to 4.9, let it stand for 24 hours and then filter to obtain a solid component. The solid component is dried at 40°C to constant weight to obtain amino acid C (cystine). ), mix amino acid A, amino acid B and amino acid C according to the mass ratio of 3.5:1.2:1.2 to obtain a compound amino acid, in which amino acid A (tyrosine), amino acid B (leucine) and amino acid C (cystine ) yields (relative to the weight of washed and dried animal hair) were 2.5%, 4.0% and 5.6% respectively, and the purity were 96%, 97% and 97% respectively.

Example 2

Prepare complex amino acids:

Hair hydrolysis: Stir and add 1.6kg of washed and dried animal hair into 2L of 7mol/L hydrochloric acid solution, and place it at 92°C for hydrolysis for 5 hours. After the hydrolysis is completed, continue to add 7mol/L hydrochloric acid solution. Until the pH of the system reaches 1.5, a hair hydrolyzate is obtained.

Amino acid extraction: Stir and add 64g of biphenyl-4-sulfonic acid (98%, purchased from Shanghai McLean Biochemical Technology Co., Ltd.) to 1L of hair hydrolyzate, mix and stir for 4 hours to obtain the first mixed liquid, and cool the first mixed liquid. to 9°C, let it stand for 24 hours and then filter to obtain the tyrosine precipitate and the first mother liquor. Then use 201×7 anion exchange resin (purchased from Tianjin Yinghai Chemical Group Co., Ltd.) to separate and purify the tyrosine precipitate. Obtain amino acid A (tyrosine); then stir and add 125g of biphenyl-4-sulfonic acid to the first mother liquid, mix and stir for 3 hours to obtain a second mixed liquid, cool the second mixed liquid to 2°C, let it stand for 36 hours, and then filter , obtain the leucine precipitate and the second mother liquor, and then use 201×7 type anion exchange resin (purchased from Tianjin Yinghai Chemical Group Co., Ltd.) to separate and purify the leucine precipitate to obtain amino acid B (leucine); Finally, stir and add 6 mol/L ammonia to the second mother liquor to adjust the pH to 5.0, let it stand for 24 hours and then filter to obtain a solid component. The solid component is dried at 42°C to constant weight to obtain amino acid C (cystine). ), mix amino acid A, amino acid B and amino acid C according to the mass ratio of 3.8:1.4:1.3 to obtain a composite amino acid, in which amino acid A (tyrosine), amino acid B (leucine) and amino acid C (cystine ) yields (relative to the weight of washed and dried animal hair) were 2.5%, 4.0% and 5.8% respectively, and the purity were 97%, 97% and 98% respectively.

Example 3

Prepare complex amino acids:

Hair hydrolysis: Stir and add 1.8kg of washed and dried animal hair into 2L of 8mol/L hydrochloric acid solution, and place it at 95°C for hydrolysis for 5 hours. After the hydrolysis is completed, continue to add 8mol/L hydrochloric acid solution. Until the pH of the system reaches 1.6, a hair hydrolyzate is obtained.

Amino acid extraction: Stir and add 70g of biphenyl-4-sulfonic acid (98%, purchased from Shanghai McLean Biochemical Technology Co., Ltd.) to 1L of hair hydrolyzate, mix and stir for 4 hours to obtain the first mixed liquid, and cool the first mixed liquid. to 10°C, let it stand for 24 hours and then filter to obtain the tyrosine precipitate and the first mother liquor. Then use 201×7 anion exchange resin (purchased from Tianjin Yinghai Chemical Group Co., Ltd.) to separate and purify the tyrosine precipitate. Obtain amino acid A (tyrosine); then stir and add 140g of biphenyl-4-sulfonic acid to the first mother liquid, mix and stir for 3 hours to obtain a second mixed liquid, cool the second mixed liquid to 4°C, let it stand for 36 hours, and then filter , obtain the leucine precipitate and the second mother liquor, and then use 201×7 type anion exchange resin (purchased from Tianjin Yinghai Chemical Group Co., Ltd.) to separate and purify the leucine precipitate to obtain amino acid B (leucine); Finally, stir and add 6 mol/L ammonia to the second mother liquor to adjust the pH to 5.1, let it stand for 24 hours and then filter to obtain a solid component. The solid component is dried at 45°C to constant weight to obtain amino acid C (cystine). ), mix amino acid A, amino acid B and amino acid C according to the mass ratio of 4.0:1.5:1.5 to obtain a composite amino acid, in which amino acid A (tyrosine), amino acid B (leucine) and amino acid C (cystine ) yields (relative to the weight of washed and dried animal hair) were 2.5%, 3.9% and 5.7% respectively, and the purity were 96%, 96% and 97% respectively.

Example 4

Preparation of complex amino acid chelated calcium:

First, rinse the surface of the shells (commercially available) with clean water to remove debris on the surface of the shells. Soak the treated shells in 2mol/L dilute hydrochloric acid for 30 minutes to dissolve the chitosan and chitin on the surface of the shells. Take out the shells and use Rinse with distilled water for 8 minutes, then dry in an oven at 80°C to constant weight, crush and pass through a 100-mesh sieve to obtain shell powder.

Stir and add 85g of shell powder to 190 mL of 2 mol/L dilute hydrochloric acid solution. Stir until the solid components are completely dissolved to obtain a calcium chloride solution. Then add 35.4g of the complex amino acid prepared in Example 1 to the calcium chloride solution. , stir and mix for 3 hours, then add NH ₃ -NHCl ₃ buffer solution with a pH of 10.4 to the system to adjust the pH to 8.9. After the pH adjustment is completed, a mixed solution is obtained. The mixed solution is heated to 65°C, stirred for 1.2 hours, and chelated. After the chelation reaction is completed, the chelating solution is obtained. The chelating solution is centrifuged at high speed for 10 minutes. The centrifugation rate of the high-speed centrifugation is set to 3500r/min. After the centrifugation is completed, the supernatant is taken and concentrated under reduced pressure, and then dialyzed with absolute ethanol. Purify for 2 days, centrifuge, and collect the precipitate to obtain complex amino acid chelated calcium.

Example 5

Preparation of complex amino acid chelated calcium:

First, rinse the surface of the shells (commercially available) with clean water to remove debris on the surface of the shells. Soak the treated shells in 2mol/L dilute hydrochloric acid for 35 minutes to dissolve the chitosan and chitin on the surface of the shells. Take out the shells and use Rinse with distilled water for 9 minutes, then dry in a 90°C oven to constant weight, crush and pass through a 100-mesh sieve to obtain shell powder.

Stir and add 88g of shell powder to 195 mL of 2 mol/L dilute hydrochloric acid solution. Stir until the solid components are completely dissolved to obtain a calcium chloride solution. Then add 35.8g of the complex amino acid prepared in Example 2 to the calcium chloride solution. , stir and mix for 4 hours, then add NH ₃ -NHCl ₃ buffer solution with a pH of 10.4 to the system to adjust the pH to 9.0. After the pH adjustment is completed, a mixed solution is obtained. The mixed solution is heated to 68°C, stirred for 1.5 hours, and chelated. After the chelation reaction is completed, the chelating solution is obtained. The chelating solution is centrifuged at high speed for 1 min. The centrifugation rate of the high-speed centrifugation is set to 3800r/min. After the centrifugation is completed, the supernatant is taken and concentrated under reduced pressure, and then dialyzed with absolute ethanol. Purify for 3 days, centrifuge, and collect the precipitate to obtain complex amino acid chelated calcium.

Example 6

Preparation of complex amino acid chelated calcium:

First, rinse the surface of the shells (commercially available) with clean water to remove debris on the surface of the shells. Soak the treated shells in 2mol/L dilute hydrochloric acid for 40 minutes to dissolve the chitosan and chitin on the surface of the shells. Take out the shells and use Rinse with distilled water for 10 minutes, then dry in a 100°C oven to constant weight, crush and pass through a 100-mesh sieve to obtain shell powder.

Stir and add 90 g of shell powder to 200 mL of 2 mol/L dilute hydrochloric acid solution. Stir until the solid components are completely dissolved to obtain a calcium chloride solution. Then add 36.0 g of the complex amino acid prepared in Example 3 to the calcium chloride solution. , stir and mix for 4 hours, then add NH ₃ -NHCl ₃ buffer solution with a pH of 10.4 to the system to adjust the pH to 9.2. After the pH adjustment is completed, a mixed solution is obtained. The mixed solution is heated to 70°C, stirred for 1.5 hours, and chelated. After the chelation reaction is completed, the chelating solution is obtained. The chelating solution is centrifuged at high speed for 12 minutes. The centrifugation rate of the high-speed centrifugation is set to 4000r/min. After the centrifugation is completed, the supernatant is taken and concentrated under reduced pressure, and then dialyzed with absolute ethanol. Purify for 3 days, centrifuge, and collect the precipitate to obtain complex amino acid chelated calcium.

Example 7

Preparation of amino acid chelated organic fertilizer:

First, amino acid chelated organic fertilizer includes the following raw materials by weight:

Among them, the film-forming liquid is prepared by the following steps:

Stir and add 35g polyvinyl alcohol (Mw89000-98000; 90% hydrolysis; viscosity 25.0-31.0mPa·s; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and 5g humic acid (fulvic acid FA≥ 90%; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.), 0.5g polyethylene grafted maleic anhydride (grafting rate 8%; average molecular weight 3000-8000; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and 0 .mL Tween-60 (purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.), stir and mix for 30 min to obtain a film-forming liquid.

Then, the production process of amino acid chelated organic fertilizer includes the following steps:

Weigh each raw material according to the weight portion, wash and dry the corn straw and wheat straw, crush and mix with a kneader, and then steam soften at 110°C for 2 hours to obtain softened straw material. Transfer the softened straw material and soybean meal to In the fermentation tank, add the fermentation strain (Grotrichia candidum yeast) and cellulase (700u/g; purchased from Sigma Aldrich (Shanghai) Trading Co., Ltd.), raise the temperature to 45°C, adjust the pH to 6.2, and ferment at a constant temperature for 36 hours. After the fermentation is completed, filter, and the solid component obtained is sterilized at 100°C for 15 minutes to obtain the fermentation component. The fermentation component and the complex amino acid chelated calcium prepared in Example 4 are stirred and mixed for 30 minutes to obtain the amino acid chelated organic fertilizer core. , then place the amino acid chelated organic fertilizer fertilizer core in a spray drum granulator, coat the surface of the amino acid chelated organic fertilizer fertilizer core with the film-forming liquid, and after drying, obtain the amino acid chelated organic fertilizer. , the particle size of amino acid chelated organic fertilizer is 3.2mm.

Example 8

Preparation of amino acid chelated organic fertilizer:

First, amino acid chelated organic fertilizer includes the following raw materials by weight:

Among them, the film-forming liquid is prepared by the following steps:

Stir and add 40g polyvinyl alcohol (Mw89000-98000; 90% hydrolysis; viscosity 25.0-31.0mPa·s; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and 6g humic acid (fulvic acid FA≥ 90%; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.), 0.8g polyethylene grafted maleic anhydride (grafting rate 8%; average molecular weight 3000-8000; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and 0.15 mL Tween-60 (purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.), stir and mix for 40 min to obtain a film-forming liquid.

Then, the production process of amino acid chelated organic fertilizer includes the following steps:

Weigh each raw material according to the weight portion, wash and dry the corn straw and wheat straw, crush and mix them with a kneader, and then steam soften them at 115°C for 3 hours to obtain softened straw material. Transfer the softened straw material and soybean meal to In the fermentation tank, add fermentation bacteria (Glococcus candidum yeast) and cellulase (700u/g; purchased from Sigma Aldrich (Shanghai) Trading Co., Ltd.), raise the temperature to 46°C, adjust the pH to 6.4, and ferment at a constant temperature for 40 hours. After the fermentation is completed, filter, and the solid component obtained is sterilized at 110°C for 18 minutes to obtain the fermentation component. The fermentation component and the complex amino acid chelated calcium prepared in Example 5 are stirred and mixed for 30 minutes to obtain the amino acid chelated organic fertilizer core. , then place the amino acid chelated organic fertilizer fertilizer core in a spray drum granulator, coat the surface of the amino acid chelated organic fertilizer fertilizer core with the film-forming liquid, and after drying, obtain the amino acid chelated organic fertilizer. , the particle size of amino acid chelated organic fertilizer is 4.0mm.

Example 9

Preparation of amino acid chelated organic fertilizer:

First, amino acid chelated organic fertilizer includes the following raw materials by weight:

Among them, the film-forming liquid is prepared by the following steps:

Stir and add 42g polyvinyl alcohol (Mw89000-98000; 90% hydrolysis; viscosity 25.0-31.0mPa·s; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and 7g humic acid (fulvic acid FA≥ 90%; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.), 1.0g polyethylene grafted maleic anhydride (grafting rate 8%; average molecular weight 3000-8000; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and 0.2 mL Tween-60 (purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.), stir and mix for 50 min to obtain a film-forming liquid.

Then, the production process of amino acid chelated organic fertilizer includes the following steps:

Weigh each raw material according to the weight portion, wash and dry the corn straw and wheat straw, crush and mix with a kneader, and then steam soften at 120°C for 3 hours to obtain softened straw material. Transfer the softened straw material and soybean meal to In the fermentation tank, add fermentation bacteria (Glococcus candidum yeast) and cellulase (700u/g; purchased from Sigma Aldrich (Shanghai) Trading Co., Ltd.), raise the temperature to 48°C, adjust the pH to 6.5, and ferment at a constant temperature for 48 hours. After the fermentation is completed, filter, and the solid component obtained is sterilized at 120°C for 20 minutes to obtain the fermentation component. The fermentation component and the complex amino acid chelated calcium prepared in Example 6 are stirred and mixed for 30 minutes to obtain the amino acid chelated organic fertilizer core. , then place the amino acid chelated organic fertilizer fertilizer core in a spray drum granulator, coat the surface of the amino acid chelated organic fertilizer fertilizer core with the film-forming liquid, and after drying, obtain the amino acid chelated organic fertilizer. , the particle size of amino acid chelated organic fertilizer is 5.0mm.

Comparative example 1

Comparative Example 1 is the control group of Example 8. In the process of preparing complex amino acid chelate calcium in Example 5 in Example 8, the complex amino acid prepared in Example 2 was replaced with amino acid A (tyrosine). The remaining preparation processes and production The process is consistent with Example 5 and Example 8, and finally an amino acid chelated organic fertilizer is obtained. The particle size of the amino acid chelated organic fertilizer is 4.0 mm.

Comparative example 2

Comparative Example 1 is the control group of Example 8. In the process of preparing complex amino acid chelate calcium in Example 5 in Example 8, the complex amino acid prepared in Example 2 was replaced with amino acid B (leucine). The remaining preparation processes and production The process is consistent with Example 5 and Example 8, and finally an amino acid chelated organic fertilizer is obtained. The particle size of the amino acid chelated organic fertilizer is 4.0 mm.

Comparative example 3

Comparative Example 1 is the control group of Example 8. In the process of preparing complex amino acid chelate calcium in Example 5 in Example 8, the complex amino acid prepared in Example 2 was replaced with amino acid C (cystine). The remaining preparation processes and production The process is consistent with Example 5 and Example 8, and finally an amino acid chelated organic fertilizer is obtained. The particle size of the amino acid chelated organic fertilizer is 4.0 mm.

Comparative example 4

Comparative Example 1 is the control group of Example 8. The film-forming liquid in Example 8 is replaced with deionized water. Then, the production process of amino acid chelated organic fertilizer includes the following steps:

Weigh each raw material according to the weight portion, wash and dry the corn straw and wheat straw, crush and mix them with a kneader, and then steam soften them at 115°C for 3 hours to obtain softened straw material. Transfer the softened straw material and soybean meal to In the fermentation tank, add fermentation bacteria (Glococcus candidum yeast) and cellulase (700u/g; purchased from Sigma Aldrich (Shanghai) Trading Co., Ltd.), raise the temperature to 46°C, adjust the pH to 6.4, and ferment at a constant temperature for 40 hours. After the fermentation is completed, filter, and the solid component obtained is sterilized at 110°C for 18 minutes to obtain the fermentation component. The fermentation component and the complex amino acid chelated calcium prepared in Example 5 are stirred and mixed for 30 minutes to obtain the amino acid chelated organic fertilizer core. , then place the amino acid chelated organic fertilizer fertilizer core in a spray drum granulator, use deionization to granulate the amino acid chelated organic fertilizer fertilizer core, and after drying, the amino acid chelated organic fertilizer fertilizer is obtained. After testing, the amino acid chelated organic fertilizer fertilizer core is The particle size of chelated organic fertilizer is 3.8mm.

Field tests were conducted on the amino acid chelated organic fertilizers prepared in Examples 4 to 6 and Comparative Examples 1 to 4. The test process was as follows, and the test results are shown in Table 1:

The test site was selected as a pepper planting demonstration field in Jiuli Town, Emeishan City, Leshan. The test site is 75m long, 9m wide, and covers an area of 675m ² . It has mountainous yellow soil, pH=4.65, organic matter 3.2-5.4g/kg, available nitrogen 55.0-71.8mg/kg, Available phosphorus 1.27-2.30mg/kg. The experimental plot was divided into several areas, each with an area of 32.5m ² , and peppers were planted in each area. The row spacing of peppers was 65cm, the plant spacing was 33cm, and about 152 plants were planted in each area. Among them, the fertilizer amount of amino acid compound micro-fertilizer is 40kg/acre. Every January, sampling (regional diagonal three-point sampling survey) is conducted to investigate the pepper plant height and leaf number.

Table 1

project Example 4 Example 5 Example 6 Comparative example 1 Comparative example 2 Comparative example 3 Comparative example 4 Plant height/0 months 18.3cm 18.4cm 18.2cm 18.4cm 18.2cm 18.2cm 18.2cm Plant height/1 month 20.7cm 20.8cm 20.6cm 20.3cm 20.5cm 20.6cm 20.4cm Plant height/2 months 22.2cm 22.4cm 22.1cm 21.8cm 22.0cm 22.2cm 21.5cm Number of leaves/0 months 13.0 pieces 13.4 pieces 13.1 pieces 13.3 pieces 13.1 pieces 13.2 pieces 13.2 pieces Number of leaves/1 month 15.4 pieces 15.7 pieces 15.5 pieces 15.2 pieces 15.4 pieces 15.4 pieces 15.3 pieces Number of leaves/2 months 16.1 pieces 16.3 pieces 16.0 pieces 15.4 pieces 15.8 pieces 16.0 pieces 14.4 pieces Growing momentum powerful powerful powerful Stronger Stronger Stronger weaker

It can be seen from Table 1 that the amino acid chelated organic fertilizer prepared by the present invention can significantly improve the fertility and action time of the fertilizer.

It should be noted that, as used herein, terms such as "comprising," or any other variations thereof are intended to cover a non-exclusive inclusion, such that a process, method, article, or device that includes a list of elements not only includes those elements, but also It also includes other elements not expressly listed or that are inherent to the process, method, article or equipment.

Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art will appreciate that various changes, modifications, substitutions and changes can be made to these embodiments without departing from the principles and spirit of the invention. Alternatively, the scope of the invention is defined by the appended claims and their equivalents.

Claims (10)

1. An amino acid chelated organic fertilizer, characterized in that it includes the following raw materials in parts by weight: 2. A kind of amino acid chelated organic fertilizer according to claim 1, characterized in that the compound amino acid chelated calcium is prepared by the following steps: Stir and add shell powder to 2mol/L dilute hydrochloric acid solution, stir until the solid components are completely dissolved to obtain a calcium chloride solution, then add complex amino acids to the calcium chloride solution, stir and mix for 3-4 hours, and then add to the system Adjust the pH to 8.9-9.2 with the NH ₃ -NHCl ₃ buffer solution with a pH of 10.4. After the pH adjustment is completed, a mixed solution is obtained. The mixed solution is heated to 65-70°C and stirred for chelation for 1.2-1.5 hours. After the chelation reaction is completed , to obtain the chelate solution, centrifuge the chelate solution at high speed for 10-12 minutes. After the centrifugation is completed, take the supernatant and concentrate it under reduced pressure, then dialyze and purify with absolute ethanol for 2-3 days, centrifuge, and take the precipitate to obtain the complex amino acid. Chelated calcium. 3. A kind of amino acid chelated organic fertilizer according to claim 2, characterized in that the dosage ratio of the dilute hydrochloric acid solution, shell powder and compound amino acid of 2mol/L is 190-200mL:85-90g:35.4- 36.0g. 4. A kind of amino acid chelated organic fertilizer according to claim 2, characterized in that, the shell powder is prepared by the following steps: Rinse the shell surface with clean water, soak the treated shells in 2mol/L dilute hydrochloric acid for 30-40 minutes to dissolve the chitosan and chitin on the shell surface, take out the shells, rinse them with distilled water for 8-10 minutes, and then place them in Dry in an oven at 80-100°C until constant weight, crush and pass through a 100-mesh sieve to obtain shell powder. 5. An amino acid chelated organic fertilizer according to claim 2, characterized in that the compound amino acid is prepared by the following steps: Step S1: Stir and add washed and dried animal hair to 6-8 mol/L hydrochloric acid solution, and place it at 90-95°C for hydrolysis for 4-5 hours. After the hydrolysis is completed, continue to add 6-8 mol/L of hydrochloric acid solution. hydrochloric acid solution until the pH of the system is 1.4-1.6 to obtain a hair hydrolyzate; Step S2: Stir and add 1/3 of the total amount of biphenyl-4-sulfonic acid to the hair hydrolyzate, mix and stir for 3-4 hours to obtain a first mixed liquid, cool the first mixed liquid to 8-10°C, and let it stand for 24 hours. Filter to obtain the tyrosine precipitate and the first mother liquor, and then use 201×7 anion exchange resin to separate and purify the tyrosine precipitate to obtain amino acid A; then stir and add the remaining biphenyl-4 to the first mother liquor. -Sulfonic acid, mix and stir for 2-3h to obtain a second mixed liquid. Cool the second mixed liquid to 0-4°C, let it stand for 36 hours and then filter it to obtain the leucine precipitate and the second mother liquid, and then use the 201×7 type anion The exchange resin is used to separate and purify the leucine precipitate to obtain amino acid B; finally, stir and add 6 mol/L ammonia water to the second mother liquor to adjust the pH to 4.9-5.1, let it stand for 24 hours and then filter, to obtain the solid component. The solid component Dry to constant weight at 40-45°C to obtain amino acid C. Mix amino acid A, amino acid B and amino acid C according to a mass ratio of 3.5-4.0:1.2-1.5:1.2-1.5 to obtain a compound amino acid. 6. A kind of amino acid chelated organic fertilizer according to claim 5, characterized in that the dosage ratio of the 6-8mol/L hydrochloric acid solution and the washed and dried animal hair described in step S1 is 2L:1.5- 1.8kg; the dosage ratio of the hair hydrolyzate and biphenyl-4-sulfonic acid described in step S2 is 1L:180-210g. 7. A kind of amino acid chelated organic fertilizer according to claim 1, characterized in that the film-forming liquid is prepared by the following steps: Stir and add polyvinyl alcohol, humic acid, polyethylene grafted maleic anhydride and Tween-60 to the deionized water, stir and mix for 30-50 minutes, and obtain a film-forming liquid. 8. A kind of amino acid chelated organic fertilizer according to claim 7, characterized in that the dosage ratio of the deionized water, polyvinyl alcohol, humic acid, polyethylene grafted maleic anhydride and Tween-60 It is 80-90mL:35-42g:5-7g:0.5-1.0g:0.1-0.2mL. 9. The production process of an amino acid chelated organic fertilizer according to any one of claims 1-8, characterized in that it includes the following steps: Weigh each raw material according to the weight portion, wash and dry the corn straw and wheat straw, crush and mix them with a kneading machine, and then steam soften them at 110-120°C for 2-3 hours to obtain softened straw material. The softened straw material Transfer the soybean meal and soybean meal to the fermentation tank, add fermentation strains and cellulase, raise the temperature to 45-48°C, adjust the pH to 6.2-6.5, ferment at a constant temperature for 36-48 hours, filter after the fermentation is completed, and place the solid component at 100- Sterilize at 120°C for 15-20 minutes to obtain the fermentation component, stir and mix the fermentation component and complex amino acid chelated calcium for 30 minutes to obtain the amino acid chelated organic fertilizer core, and then place the amino acid chelated organic fertilizer core in the spray transfer In the drum granulator, the film-forming liquid is coated on the surface of the amino acid chelated organic fertilizer fertilizer core, and after drying, the amino acid chelated organic fertilizer is obtained. 10. The production process of an amino acid chelated organic fertilizer according to claim 9, characterized in that the fermentation strain is Geotrichum candidum yeast.