CN115282096B - A kind of essence for removing acne marks and preparation method thereof - Google Patents

Abstract

The invention discloses acne mark removing essence and a preparation method thereof. The essence mainly comprises the following components in percentage by mass: 12-18% of a nanostructure lipid carrier, 1.5-3% of a humectant, 0.5-1.2% of an anti-fouling agent, 0.1-0.15% of an anti-allergic agent, 0.1-0.5% of a preservative, 0.5-1% of a pH regulator, 0.01-0.02% of a aromatic agent and the balance of water. The invention adopts the nano-carrier technology to effectively promote the percutaneous permeation of the plant active ingredients and obviously increase the retention of the skin on the active ingredients. Meanwhile, the nano carrier can effectively improve the solubility of insoluble active ingredients, enhance the stability of the active ingredients, realize the synergistic inhibition of tyrosinase by various active ingredients so as to prevent the formation of melanin, promote the catabolism of formed melanin precipitation and achieve the effect of fading acne marks.

Description

A kind of essence for removing acne marks and preparation method thereof

technical field

The invention relates to the technical field of cosmetics, in particular to a novel essence for removing acne marks and a preparation method thereof.

Background technique

As we all know, the skin is the most vulnerable part of the body, and the daily exposure of human skin will cause many problems, such as acne, acne marks, pimples, pigmentation and sunburn marks, etc. The most common skin disease nowadays is acne. If the patient touches the affected area with his hands, does not pay attention to diet, often stays up late, uses functional products casually, is exposed to air pollutants and ultraviolet radiation, etc. during the period of acne, it will cause acne. A series of skin problems such as acne marks, acne pits, melanosis, inflammation, damaged oxidation and damaged barrier function have a great impact on the overall facial appearance and skin condition, and bring great psychological pressure to patients. even social barriers.

At present, a variety of products with the function of removing acne marks have been developed for the problem of acne marks. However, these products may use a large number of ingredients such as antibiotics, hormones, and heavy metals, which are costly, have side effects, and are toxic. Although short-term use of such products can effectively inhibit bacteria, anti-inflammation, and lighten acne marks, but long-term use may cause allergic reactions, toxic reactions, inflammation and infection and other problems. As a result, many consumers are rapidly increasing their demand for skin care products containing natural ingredients. However, plant active ingredients have problems such as poor solubility, poor stability, and low skin permeability in the application of acne scar products, which limit the application of plant active ingredients in acne scar products. Fortunately, the nano-carrier technology has solved the above problems well and greatly expanded the application of plant active ingredients in anti-acne products, but the existing nano-liposomes are still unstable.

Contents of the invention

In order to solve the above problems, the object of the present invention is to provide a novel anti-acne essence and its preparation method.

For solving its technical problem, the technical scheme that the present invention adopts is:

The invention provides a new type of essence for removing acne marks, which is characterized in that it comprises the following components in mass percent: 12-18% of nano-structured lipid carrier, 1.5-3% of moisturizing agent, 0.5-1.2% of anti-fouling agent %, antisensitizer 0.1~0.15%, preservative 0.1~0.5%, pH regulator 0.5~1%, fragrance 0.01~0.02%, balance water.

Preferably, the novel acne-removing print essence is characterized in that the nano-structured lipid carrier is 0.5-3% of licorice extract, 1-5% of chamomile extract, 1-5% of grape seed extract %; according to the mass percentage, it also includes 3~5% of glycerol monolaurate, 3~5% of calendula oil, and 0.5~1% of hydrogel.

Preferably, the hydrogel is composed of polyethylene glycol stearate, chitosan, acrylic acid and N,N-methylenebisacrylamide; chitosan in the hydrogel: polyethylene glycol The mass ratio of stearate:acrylic acid:N,N-methylenebisacrylamide is 1:5:15:2.

Preferably, the novel anti-acne and print essence is characterized in that the moisturizing agent is 0.5-1.5% hyaluronic acid and 0.5-1.2% tremella polysaccharide.

Preferably, the new type of essence for removing acne marks is characterized in that the antifouling agent is 0.03-0.09% of vitamin C, 0.01-0.08% of vitamin E and 0.4-1% of ferulic acid.

Preferably, the novel anti-acne and print essence is characterized in that the anti-sensitivity agent is 0.05-0.09% of Swert sprout extract and 0.02-0.07% of Coptis chinensis extract.

Preferably, the novel anti-acne essence essence is characterized in that the preservative is 0.1-0.5% of methylparaben.

Preferably, the new type of essence for removing acne marks is characterized in that the pH regulator is 0.5-1% triethanolamine.

Preferably, the novel acne-removing print essence is characterized in that the licorice extract contains quercetin and licorice flavonoids; the chamomile extract contains flavonoid active ingredients; the grape seed extract Contains proanthocyanidins.

The present invention also provides a preparation method of the novel acne-removing and printing essence, which is characterized in that it comprises the following steps:

(1) Prepare a solution containing nanostructured lipid carrier (NLC): first heat glycerol monolaurate and calendula oil to 50~60°C, then licorice extract, chamomile extract, grape seed extract Add to the oil phase, and finally heat the water phase containing the hydrogel (the water content in the water phase is: 40% deionized water in the balance) to 50~60°C, and pour it into the oil phase; further ultrasonicate the mixture Process, be cooled to 25 DEG C, obtain nanostructured lipid carrier (NLC); And it is stored at 4 DEG C for subsequent use;

The hydrogel is composed of polyethylene glycol stearate, chitosan, acrylic acid and N,N-methylenebisacrylamide, and the chitosan in the hydrogel: polyethylene glycol stearic acid The mass ratio of ester: acrylic acid: N, N-methylene bisacrylamide is: 1:5:15:2, and the preparation method of the hydrogel is: an acidic solution of chitosan, polyethylene glycol stearyl Acrylic acid ester and acrylic acid were added to the three-necked flask, under the protection of nitrogen, at a temperature of 50~60°C, stirring and dropping the ethanol solution of azobisisobutyronitrile and N,N-methylene acrylamide, and reacting for 5~8h Finally, the product is soaked and washed with deionized water, dried, and ground into powder; the acidic solution of chitosan is a formic acid solution with a mass fraction of 1% chitosan or an acetic acid solution with a mass fraction of 1% chitosan ; The volume mass fraction of azobisisobutyronitrile in the ethanol solution of said azobisisobutyronitrile is 0.01g/mL; the mass ratio of said azobisisobutyronitrile and acrylic acid is 0.005～0.02:1;

(2) Prepare Acne Removing Essence: first add the anti-sensitizer to 50% of the remaining deionized water, stir at 60-90°C for 10-40 minutes, then add moisturizer and anti-fouling agent and continue stirring for 10-40 minutes , completely dissolved and then cooled to 40~60°C; then add the NLC prepared in (1) above, and stir at 60~90°C for 10~40min; Add the aqueous solution of the pH regulator and the remaining deionized water to the essence, stir evenly, and adjust the pH value to 6~6.5, and discharge.

The present invention provides the application of the anti-acne mark essence described in the above solution or the essence prepared by the preparation method described in the above technical solution in the anti-acne mark.

Compared with prior art, the beneficial effect of the present invention is:

1. A kind of novel anti-acne printing essence prepared by the present invention, quercetin and licorice flavonoids in the licorice extract can inhibit the activity of tyrosinase and dopachrome interconversion enzyme (TRP-2), hinder The polymerization of 5,6-dihydroxyindole (DHl) prevents the formation of melanin, thereby achieving the effect of whitening the skin.

2. A new type of essence for removing acne marks prepared by the present invention, the chamomile extract is rich in flavonoid active ingredients, which can scavenge free radicals and inhibit tyrosinase activity. At the same time, the ingredient also has antioxidant, anti-inflammatory, anti-allergic, and anti-viral effects.

3. A new type of acne-removing essence prepared by the present invention, the proanthocyanidins contained in the grape extract have super antioxidant capacity, protect collagen, improve skin elasticity and luster, whiten, moisturize, and freckle; reduce wrinkles and maintain skin Soft and smooth; clear acne, heal scars.

4. A new type of acne-removing print essence prepared by the present invention, the polysaccharide in the extract contains many hydrophilic hydroxyl groups, which can form hydrogen bonds with water molecules and cross-link each other into a network structure to make it perform well. water absorption and moisture retention.

5. A new type of essence for removing acne marks prepared by the present invention, by adding natural ingredients for removing acne marks, and simultaneously adding auxiliary agents such as antifouling agent and penetration enhancer, a kind of essence with good effect of removing acne marks is prepared. The nano-carrier technology can effectively promote the percutaneous penetration of plant active ingredients and significantly increase the skin's retention of active ingredients. At the same time, nano-carriers can effectively improve the solubility of insoluble active ingredients, enhance the stability of active ingredients, and realize the synergistic inhibition of tyrosinase by multiple active ingredients, so as to prevent the formation of melanin and promote the catabolism of melanin deposits that have formed , so as to achieve the effect of diluting acne marks, and the hydrogel on the nano-carrier can increase the stability of liposomes.

6. The present invention prepares hydrogel by preparing polyethylene glycol stearate, chitosan, acrylic acid and N,N-methylenebisacrylamide, and loads it on the outer layer of nano-lipid, wherein polyethylene The hydrophobic long carbon chain of diol stearate can be embedded between liposomes to reduce leakage. The charge repulsion carried by chitosan is conducive to the stability of liposomes and prevents aggregation; at the same time, the charge carried on the surface of chitosan is conducive to combining with cells and increasing the adsorption capacity. At the same time, polyacrylic acid has excellent water retention.

Description of drawings

Fig. 1 is embodiment 1～4 and comparative example 1～5 tyrosinase activity inhibition experimental figure;

Fig. 2 is a graph showing the penetration amount of the printing removing active substance in the stratum corneum of Examples 1-4 and Comparative Examples 1-5 at different time points.

Implementation

The present invention is described in detail through the following examples, which are only used to further illustrate the present invention, and should not be construed as limiting the protection scope of the present invention. It should be understood that after reading the contents of the present invention, those skilled in the art may make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

Embodiment 1～4:

Embodiments 1 to 4 provide a novel acne-removing essence, which are composed of the components shown in Table 1 in percentage by weight:

Table 1 The content ratio of each case of acne-removing and printing essence

Note: In the present invention, the balance refers to the limited weight percentage of components other than ionized water in the composition of the essence formula. After the content of all components in the formula is added, it is finally made up to 100% with deionized water . Essence is daily essence.

It should be noted that the nanostructured lipid carrier of the present invention is 0.5-3% of licorice extract, 1-5% of chamomile extract, and 1-5% of grape seed extract; Laurate 3~5%, calendula oil 3~5%, hydrogel 0.5~1%. In the preparation process of the nanostructured lipid carrier, an aqueous phase needs to be added, which is 40% of the mass fraction of the remaining water, and the aqueous solution of the nanostructured lipid carrier is obtained.

The preparation method of embodiment 1～4 hydrogel is: 1g chitosan is stirred and dissolved in 100mL mass fraction in the there-necked flask and is 1% acetic acid solution, then add 5g polyethylene glycol stearate, 15g acrylic acid is added To a three-necked flask, under nitrogen protection, at a temperature of 50-60°C, stir and drop 10mL of azobisisobutyronitrile ethanol solution with a volume mass fraction of 0.01g/mL and 2g of N,N-methyleneacrylamide , After reacting for 5~8h, soak and wash the product with deionized water, dry, and grind into powder.

The preparation method of the acne-removing print essence of embodiment 1-4 comprises the following steps:

(1) Preparation of a solution containing nanostructured lipid carrier (NLC): first, glycerol monolaurate and calendula oil were heated to 55°C, then licorice extract, chamomile extract, and grape seed extract were added to the oil phase, and finally the water phase containing the hydrogel (the water content in the water phase: 40% deionized water in the balance) was heated to 55°C and poured into the oil phase; the mixture was further sonicated and cooled to 25°C to obtain the nanostructured lipid carrier (NLC); and store it at 4°C for future use.

(2) Preparation of Acne Removing Essence: First add the anti-sensitizer to 50% of the remaining deionized water, stir at 60-90°C for 20 minutes, then add moisturizer and anti-fouling agent and continue stirring for 20 minutes, after completely dissolving Cool to 40°C; then add the NLC prepared in (1) above, and stir at 70°C for 20 minutes; when the temperature drops to 40°C, add preservatives and fragrances at a time, stir evenly, and mix the aqueous solution of the pH regulator with Add the remaining deionized water into the essence together, adjust the pH value to 6.5, and discharge.

Comparative example 1

This comparative example is a new type of anti-acne essence, its raw materials and preparation method are similar to Example 1, the difference is that the tremella polysaccharide, grape seed extract and licorice extract are not used.

Comparative example 2

This comparative example is a new type of anti-acne essence, its raw materials and preparation method are similar to Example 1, the difference is that the Swert sprout extract, grape seed extract and chamomile extract are not used.

Comparative example 3

This comparative example is a novel anti-acne essence, its raw materials and preparation method are similar to Example 1, the difference is: the nanostructured lipid carrier (NLC) is not used, and its preparation method is to extract licorice extract and chamomile Add substances, grape seed extract, and anti-sensitizer to the water phase, stir at 70°C for 20 minutes, then add humectant and anti-fouling agent and continue stirring for 20 minutes, after completely dissolving and cooling down to 40°C, add preservatives and fragrance at one time Add the aqueous solution of the pH regulator and the remaining deionized water to the essence, stir evenly, and adjust the pH value to 6.5, then discharge.

Comparative example 4

This comparative example is a new type of anti-acne essence, its raw materials and preparation method are similar to Example 1, the difference is that no polyethylene glycol stearate is added during the preparation of the hydrogel.

Comparative example 5

This comparative example is a novel anti-acne essence, its raw materials and preparation method are similar to Example 1, the difference is that no chitosan is added in the preparation process of the hydrogel.

Physical and chemical performance test:

Test the pH value, high and low temperature stability and centrifugal stability of the product to evaluate the physical and chemical properties of the product. Using the dilution method, use a pH meter to measure the pH value of the product: Accurately weigh 10.0 g of the developed acne-removing conditioning essence milk, add 10.0 g of deionized water after boiling and cooling to dilute, heat to 40°C, and keep stirring during the period Disperse it evenly, then cool it to room temperature, insert a calibrated pH meter, read the value after the reading is stable, and take the average value of two measurements. High and low temperature stability test: put the product in a -15°C refrigerator for 24 hours, take it out and place it in a 45°C oven for 24 hours, repeat 3 times, and observe the state of the product.

Physical and chemical performance test results:

The physical and chemical properties of the acne-removing and conditioning essence milk prepared according to the final formula were tested, and the pH value was 6.0-6.5. After high and low temperature and centrifugation experiments, the stability was good, and no oil-water separation was observed.

Irritation test, allergic test:

The hair on the back of the mouse was cut off, and the essence prepared according to Examples 1-4 and Comparative Examples 1-5 were applied to the cut-off area respectively, and compared with the uncoated area, the results showed no irritation and allergic reaction. Get 1 gram of the essence liquid that embodiment 1～4 and comparative example 1～5 gained are applied on the hands of volunteers, observe after 30 minutes, all do not appear rubescent, eruption, bubbling phenomenon.

Effect Test of Acne Removing Essence

Test population: The subjects were all college student volunteers suffering from acne marks, including 135 male and female students in grades one to four.

Preparations before the test: The subject is not allowed to use any products (cosmetics or external drugs) on the test site within 3 days before the test. Before the official test, the basic test is carried out on the volunteers, and an appropriate amount of this product is applied to the back of the volunteers' ears to observe whether they have allergies or other adverse reactions. Only if all goes well can they sign an informed consent to continue taking the test.

Test method: randomly divided into 9 groups, the trial period is 28 days, the samples of the control group are smeared on the left cheek, and the samples of the test group are smeared on the right cheek. Follow-up visits were made on the first day and the 28th day respectively, and the follow-up day was at the same time, and the subjective evaluation data of the subjects were collected.

Judging criteria: 15 subjects in each group are asked to score the acne scar-removing effect of the products of Examples 1-4 and Comparative Examples 1-5 with the naked eye based on their own use conditions, and they are divided into invalid, insignificant effect, general effect and effective There are obviously 4 grades in total, the results are shown in Table 2:

Table 2 Trial effect of Acne Removing and Printing Essence

The experimental method for tyrosinase activity inhibition is: T/SHRH 015, and the experimental results are shown in Figure 1. It can be seen that the anti-acne mark components in Comparative Example 1 and Comparative Example 2 are single, there is no liposome in Comparative Example 3, and the liposomes in Comparative Example 4 and Comparative Example 5 are not as stable as Examples 1-4. Comparative examples 1～5 tyrosinase activity inhibitory effect is not as good as embodiment.

In vitro skin penetration test: Confocal Raman technology was used to detect the skin permeability of the skin after a single use of the essence for 15 minutes, 1 hour and 2 hours in the test area of the subject, and the concentration of the essence in the stratum corneum at different time points was obtained. See Figure 2 for the penetration rate of print removal actives. It can be seen that with the prolongation of the single use time of the essence, the penetration amount gradually increases. Comparative Example 3 has low penetration due to no nano lipid carrier. Comparative example 4 has no polyethylene glycol stearate, and comparative example 5 has no chitosan, and the liposome stability effect is not as good as that of Examples, and the penetration is low.

Investigation on the Comprehensive Effect of the Essence of the Present Invention

Evaluate the efficacy of the anti-acne print essence prepared in Examples 1-4 and Comparative Examples 1-5 by using the trial feeling. Using the public opinion survey scoring method, 90 college student volunteers were recruited as trial subjects, divided into 9 groups, and the essence prepared in Examples 1-4 and Comparative Examples 1-5 were applied to the acne marks. Use twice a day for a month. The total score of each use effect is 10 points: 10 is the highest score, which means very good and very satisfied; 8 is good; 6 is acceptable; below 6 is bad and unacceptable. The scoring data of each group is averaged, and the results are shown in Table 3:

Table 3 comprehensive use effect

In summary, the essence prepared by the present invention can effectively promote the percutaneous penetration of plant active ingredients, and significantly increase the skin's retention of active ingredients. At the same time, it effectively improves the solubility of insoluble active ingredients, enhances the stability of active ingredients, and realizes the synergistic inhibition of tyrosinase by various active ingredients, so as to prevent the formation of melanin and promote the catabolism of melanin deposits that have formed, so as to reduce acne. printing effect.

The above are only specific embodiments of the present invention, but the technical features of the present invention are not limited thereto. Any simple changes, equivalent replacements or modifications based on the present invention to solve basically the same technical problems and achieve basically the same technical effects are covered by the protection scope of the present invention.

Claims (8)

1. The acne mark removing essence is characterized by comprising the following components in percentage by mass: 12-18% of a nanostructure lipid carrier, 1.5-3% of a humectant, 0.5-1.2% of an anti-fouling agent, 0.1-0.15% of an anti-sensitization agent, 0.1-0.5% of a preservative, 0.5-1% of a pH regulator, 0.01-0.02% of a aromatic agent and the balance of water;

the anti-fouling agent consists of vitamin C, vitamin E and ferulic acid;

the antiallergic agent comprises swertia extract and Coptidis rhizoma extract;

the water in the rest water is deionized water;

the nanostructure lipid carrier comprises, by mass, 0.5-3% of licorice extract, 1-5% of chamomile extract, 1-5% of grape seed extract, 3-5% of glycerol monolaurate, 3-5% of calendula oil and 0.5-1% of hydrogel;

the hydrogel consists of chitosan, polyethylene glycol stearate, acrylic acid and N, N-methylene bisacrylamide in a mass ratio of 1:5:15:2;

the preparation method of the hydrogel comprises the following steps: adding an acidic solution of chitosan, polyethylene glycol stearate and acrylic acid into a three-neck flask, stirring and dropwise adding an ethanol solution of azodiisobutyronitrile and N, N-methylene acrylamide at the temperature of 50-60 ℃ under the protection of nitrogen, reacting for 5-8 hours, soaking and washing the product with deionized water, drying, and grinding into powder; the acidic solution of the chitosan is a formic acid solution of which the mass fraction is 1% of chitosan or an acetic acid solution of which the mass fraction is 1% of chitosan; the volume mass fraction of the azobisisobutyronitrile in the ethanol solution of the azobisisobutyronitrile is 0.01g/mL; the mass ratio of the azodiisobutyronitrile to the acrylic acid is 0.005-0.02:1;

the preparation method of the Nanostructure Lipid Carrier (NLC) comprises the following steps: firstly, heating glycerol monolaurate and calendula oil to 50-60 ℃, then adding licorice extract, chamomile extract and grape seed extract into an oil phase, finally heating a water phase containing hydrogel to 50-60 ℃, and pouring the mixture into the oil phase, wherein the water content in the water phase is 40% of the rest water; further subjecting the mixture to ultrasonic treatment and cooling to 25 ℃ to obtain a solution containing the Nanostructure Lipid Carrier (NLC); and stored at 4 ℃ for later use.

2. The acne mark removing essence according to claim 1, wherein the humectant is 0.5-1.5% of hyaluronic acid and 0.5-1.2% of tremella polysaccharide.

3. The acne mark removal essence according to claim 1, wherein the anti-fouling agent comprises 0.03-0.09% of vitamin C, 0.01-0.08% of vitamin E and 0.4-1% of ferulic acid.

4. The acne mark removing essence according to claim 1, wherein the antiallergic agent comprises 0.05-0.09% of swertia extract and 0.02-0.07% of coptis extract.

5. The acne mark removing essence according to claim 1, wherein the preservative is 0.1-0.3% of methylparaben.

6. The acne mark removing essence according to claim 1, wherein the pH regulator is triethanolamine 0.5-1%.

7. A method for preparing the acne mark removing essence according to any one of claims 1 to 6, comprising the steps of: firstly adding an anti-allergic agent into 50% of the rest deionized water, stirring for 10-40 min at 60-90 ℃, then adding a humectant and an anti-fouling agent, continuously stirring for 10-40 min, completely dissolving, and cooling to 40-60 ℃; adding a solution containing a Nanostructure Lipid Carrier (NLC) into the solution, and stirring the solution at 60-90 ℃ for 10-40 min; and (3) when the temperature is reduced to 35-55 ℃, adding the preservative and the aromatic agent at one time, uniformly stirring, adding the aqueous solution of the pH regulator and the rest deionized water into the essence, regulating the pH value to be 6-6.5, and discharging.

8. Use of the acne mark removing essence according to any one of claims 1 to 6 or the essence prepared by the preparation method of claim 7 for preparing an acne mark removing product.