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CN118121524B - Acne-removing facial cleanser composition and bath foam thereof - Google Patents

Acne-removing facial cleanser composition and bath foam thereof Download PDF

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Publication number
CN118121524B
CN118121524B CN202410551852.3A CN202410551852A CN118121524B CN 118121524 B CN118121524 B CN 118121524B CN 202410551852 A CN202410551852 A CN 202410551852A CN 118121524 B CN118121524 B CN 118121524B
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acne
parts
extract
agent
skin
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CN118121524A (en
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吴杰峰
林剑森
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Guangdong Songtaixing Cosmetics Co ltd
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Guangdong Songtaixing Cosmetics Co ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
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    • A61K8/33Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34Alcohols
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    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
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    • A61K8/33Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36Carboxylic acids; Salts or anhydrides thereof
    • A61K8/368Carboxylic acids; Salts or anhydrides thereof with carboxyl groups directly bound to carbon atoms of aromatic rings
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    • A61K8/44Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K8/44Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A61K8/442Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof substituted by amido group(s)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K8/00Cosmetics or similar toiletry preparations
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    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60Sugars; Derivatives thereof
    • A61K8/602Glycosides, e.g. rutin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K8/60Sugars; Derivatives thereof
    • A61K8/604Alkylpolyglycosides; Derivatives thereof, e.g. esters
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • A61K8/738Cyclodextrins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/84Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
    • A61K8/86Polyethers
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    • A61K8/9728Fungi, e.g. yeasts
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    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
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Abstract

The invention relates to the technical field of cosmetics, in particular to an acne-removing facial cleanser composition and shower gel thereof. It comprises salicylic acid, cyclodextrin, flos Chrysanthemi Indici extract, herba Taraxaci extract, radix Paeoniae extract, flos Matricariae Chamomillae extract, flos Mali Pumilae extract, aloe vera leaf juice, tremella fruiting body extract, etc. The shower gel disclosed by the invention has excellent cleaning and acne removing performances. The body wash has no obvious reddening, mild and no stimulation in the body wash patch test.

Description

Acne-removing facial cleanser composition and bath foam thereof
Technical Field
The invention relates to the technical field of cosmetics, in particular to an acne-removing facial cleanser composition and shower gel thereof.
Background
Along with the acceleration of modern life rhythm and the improvement of health consciousness of people, the quality and performance of the shower gel are increasingly focused by consumers as an indispensable cleaning product in daily life. Although the traditional surfactant bath cream can meet the basic cleaning requirement, the traditional surfactant bath cream cannot meet the pursuit of modern people on life quality due to various problems in the use process, such as uncleanness in washing, trace toxic substances generation and the like. The fatty acid soap base type bath lotion has better flushing property, but has over-strong alkalinity, is easy to cause irritation to skin, makes the skin feel dry and tight, even causes itching, causes inflammation, red swelling and the like. Therefore, developing a shower gel which is mild in performance, good in flushing performance, and capable of removing acnes at the same time, and capable of cleaning the face becomes a new market demand.
Disclosure of Invention
In order to achieve the aim of the invention, the invention provides an acne-removing facial cleanser composition, which comprises an acne-removing agent, a conditioner and a cleanser.
The acne removing agent comprises the following main components:
Salicylic acid (SALICYLIC ACID) has very wide application in cosmetics, and has the effects of cleaning, removing cutin, removing acne, shrinking pores, tightening skin, whitening skin, brightening skin and the like because of the special lipid solubility, antibiosis, anti-inflammatory, skin cell renewal stimulation, melanin formation inhibition and the like.
CYCLODEXTRIN (CYCLODEXTRIN), a cyclic oligomer formed by specific fermentation of starch, can form a water-soluble inclusion compound with various lipophilic substances, so that the solubility of the substances in water is improved, and on the basis of the CYCLODEXTRIN, CYCLODEXTRIN is commonly used as a transmission medium to increase the transdermal absorption capacity of cosmetics, and has a certain antibacterial and anti-aging effects, so that the CYCLODEXTRIN is widely applied to the cosmetics.
The wild chrysanthemum flower (CHRYSANTHEMUM INDICUM) flower extract is extracted from the corolla part of wild chrysanthemum flower, is a natural active ingredient widely applied to cosmetics and health products, mainly plays the role of skin conditioning agent in skin care cosmetics, has the effects of resisting oxidation and inflammation, can be used for treating symptoms such as skin itch and eczema, can avoid skin dryness and can prevent skin aging.
Herba Taraxaci (TARAXACUM MONGOLICUM) extract is prepared from herba Taraxaci by concentrating, spray drying, and other processes. Mainly contains dandelion sterol, choline, inulin, pectin and other components. In the field of cosmetics, dandelion extracts can be used for preparing facial masks, face creams, essence and the like due to the effects of anti-inflammatory, antioxidation, moisturizing and the like, and are beneficial to improving skin states and increasing skin elasticity and luster.
Paeonia lactiflora (PAEONIA LACTIFLORA) root extract, paeonia lactiflora root extract, i.e. Paeonia lactiflora root, is the root of Paeonia lactiflora of Ranunculaceae, and has high medicinal value. It has strong antibacterial effect, and can inhibit elastase activity, promote fibroblast activity, eliminate free radical, and promote cathepsin activity, and the radix Paeoniae extract has skin activating and antiaging effects, and can be used in cosmetics. In addition, the paeonia lactiflora root extract can be used as a humectant, an antiallergic agent and an anti-inflammatory agent, and has remarkable effects on moisturizing, antiallergic and anti-inflammatory of skin.
The conditioning agent comprises:
The active ingredient extracted from the chamomile (chamomile) which is an extract of chamomile (CHAMOMILLA RECUTITA) has various effects and wide application fields. The flos Matricariae Chamomillae extract is rich in volatile oil, flavonoids, amino acids, chlorogenic acid and microelements, and the components endow the flos Matricariae Chamomillae extract with antibacterial, antioxidant, antiinflammatory, relieving and antiallergic biological activities. Can be used as nutritional additive for preventing allergy, keeping moisture, preventing acne, and resisting aging. In addition, the skin moisturizing agent can be used for moisturizing a large amount of skin, reducing fine wrinkles, relieving the skin, resisting skin sensitivity, effectively supplementing water to the skin, enabling the skin to be in a relatively stable state and reducing the problem of redness of the skin.
The flower extract of Malva sylvestris (MALVA SYLVESTRIS) is a natural plant extract and contains rich flavonoid, polysaccharide, organic acid and other components.
Aloe vera (Aloe BARBADENSIS) leaf juice is a natural plant extract and has various effects and application fields. Can be directly used for skin care, such as anti-inflammation, sterilizing, and relieving mosquito bite or local pruritus. Meanwhile, the skin care product can also be prepared to provide skin with moisture.
Tremella (TREMELLA FUCIFORMIS) fruiting body extract: the tremella fruit body extract has wide application in the cosmetic field. The skin-care product can form a protective film to cover the surface of the skin, plays a role in moisturizing and nourishing, and effectively relieves skin dryness and moisture loss. In addition, the product has the effects of resisting inflammation, detumescence, whitening skin, reducing speckle, enhancing cellular immunity, scavenging free radicals, resisting oxidation, and resisting aging. These effects are mainly due to the active ingredients in tremella fruit body extract, such as colloid substances, amino acids, multiple vitamins, etc.
Xylitol-based glucoside (XYLITYLGLUCOSIDE), a glycan consisting of xylitol and glucoside. It has a variety of unique properties and a wide range of applications. Xylitol-based glucosides are a common ingredient in cosmetic and personal care products. It can be used as humectant to form a moisturizing film, reduce water loss, and increase skin water retention. Meanwhile, the anti-aging agent also has an anti-oxidation effect, can neutralize free radicals, reduce skin damage and achieve an anti-aging effect. In addition, xylitol-based glucoside can also regulate skin pH value, reduce skin sensitivity to external stimulus, and relieve skin.
Xylitol (XYLITOL): xylitol is an organic compound, also known as methylaldehyde or papaya sugar alcohol, and has the chemical formula C 5H12O5. Is native to Finnish, and can be extracted from plant materials such as birch, oak, corncob, bagasse, etc., and xylitol has effects of keeping moisture, resisting oxidation, and relieving inflammation in cosmetic field.
The cleaning agent comprises:
Ammonium laureth sulfate (Laureth Sulfate Ammonium): a compound is prepared from laureth and sulfuric acid. It has good solubility and can form transparent solution in water. Is widely used in shampoo, foam bath, shower foam and other preparations requiring high foam and good skin tolerance.
Cocamidopropyl betaine (Cocoamidopropyl Betaine): a mild amphoteric surfactant is derived from coconut oil. Has good synergistic effect, can reduce the irritation generated by the traditional surfactant, improve the foaming force and generate fine, stable and rich foam. In addition, it has thickening effect and less skin irritation.
Sodium lauroyl sarcosinate (sodium N-lauroylsarcosinate): a white to pale yellow liquid has special smell, and is dissolved in water, ethanol or glycerol. Has less skin irritation and weak degreasing effect, and is stable to heat, acid and alkali.
Lauroyl amphoacetate sodium (sodium lauroamphoacetate): the amphoteric ion surfactant is colorless to amber viscous liquid, has low irritation to skin and eyes, and is a mild surfactant. Hard water resistance, and makes the foam rich, fine and stable.
Sodium cocoyl methyl taurate (SODIUM METHYL COCOYL TAURATE): a novel amino acid surfactant is prepared by condensing natural fatty acid and sodium methyltaurine. Has very little irritation to skin. Is often used for preparing various high-grade cosmetics, infant products, facial cleanser, foam bath lotion and the like.
Decyl glucoside (n-Decyl glucoside): also called n-decyl glucoside, is one of novel nonionic surfactants APG. It is easy to dissolve in water, low in surface tension, rich in foam, fine and stable, non-toxic, harmless, non-irritating and quick in biological degradation. Has excellent washing, wetting, emulsifying and dispersing abilities, and is a green surfactant with comprehensive performance.
The acne-removing facial cleanser is prepared by compounding an acne-removing agent, a conditioner and a cleaning agent. Experiments prove that the acne-removing agent, the conditioner and the cleaning agent interact with each other to play a role in synergy, so that the acne-removing facial cleaning composition has better efficacy.
Preferably, the acne-removing cleansing composition comprises the following raw materials in percentage by mass: 1-1.5 parts of acne removing agent, 0.4-1 part of conditioner and 27-30 parts of cleaning agent.
Preferably, the acne removing agent comprises the following raw materials in parts by mass: 5.8-7 parts of salicylic acid; 0.4-1 part of cyclodextrin; 0.6-1 part of wild chrysanthemum extract; 0.6-1 part of dandelion extract; 0.6-1 part of paeonia lactiflora root extract.
Preferably, the conditioning agent comprises the following raw materials in parts by mass: 0.3-1 part of chamomile extract; 0.3-1 part of Malva sylvestris extract; 1.8-2 parts of aloe vera leaf juice; 3.5-4 parts of tremella fruit body extract; xylitol-based glucoside 0.045-0.05; xylitol 0.035-0.05.
Preferably, the cleaning agent comprises the following raw materials in percentage by mass: 10.5-11 parts of laureth ammonium sulfate; 1.05-1.1 parts of cocamidopropyl betaine; 0.9-1 part of sodium lauroyl sarcosinate; 0.95-0.97 parts of lauroyl amphoacetate sodium; 0.35-0.4 part of cocoyl methyl taurine sodium; 0.55-0.7 part of decyl glucoside.
Preferably, the acne-removing cleansing composition further comprises a preservative, a thickener and a chelating agent, wherein the preservative is phenoxyethanol and/or sodium benzoate; the thickener is sodium chloride; the chelating agent is disodium EDTA.
Preferably, the preservative is a mixed solution of phenoxyethanol and sodium benzoate in any proportion.
Experiments show that when the acne removing agent, the conditioner and the cleaning agent are compounded according to the mass part ratio, the acne removing cleaning effect is better.
The invention also provides application of the acne-removing cleansing composition in cosmetics.
The invention also provides a cosmetic which contains the acne-removing cleansing composition. The cosmetic can be in the form of cream, lotion, essence, facial mask, bath lotion, etc., but is not limited thereto.
Preferably, the acne-removing cleansing composition comprises 20-40 wt% of cosmetics.
When the acne-removing facial cleaning composition is applied to cosmetics in the weight percentage, the acne-removing facial cleaning composition can provide cosmetics with better acne-removing facial cleaning effect.
Most preferably, the acne-removing cleansing composition comprises 35 weight percent of the acne-removing cleansing composition in cosmetics, and the acne-removing cleansing composition has the best acne-removing cleansing effect when added in the acne-removing cleansing composition.
When the acne-removing facial cleaning composition is applied to cosmetics in the weight percentage, the acne-removing facial cleaning effect of the cosmetics can be effectively improved.
The invention also provides acne-removing cleansing shower gel which comprises the following components in percentage by weight:
20% -45% of the acne-removing facial cleanser composition and the balance of water are filled. The acne-removing cleansing shower gel prepared by the formula disclosed by the invention is mild and free of irritation to skin, is safe to use, has excellent acne-removing cleansing efficacy, and is suitable for various skin types.
More preferably, the acne-removing cleansing composition bath cream comprises the following components in percentage by weight:
35% of the acne-removing cleansing composition and the balance of deionized water. The skin care product bath cream prepared by the formula is mild and has no stimulation to skin, is safe to use, has excellent acne-removing and face-cleaning effects, and is suitable for various skin types.
Preferably, the preparation method of the acne-removing facial cleansing shower gel comprises the following steps of:
s1, adding a cleaning agent and a chelating agent into water, heating to 80-85 ℃, stirring and cooling;
And S2, after cooling to 40-45 ℃, adding an acne removing agent, a conditioner, a preservative and a thickener into the S1, stirring and dissolving until the temperature is less than or equal to 38 ℃, and standing for 12 hours.
Compared with the prior art, the invention has the beneficial effects that; the invention creatively adds acne removing and skin conditioning components into the bath lotion, and improves the absorption of various natural product extracts and various beneficial components in the bath lotion while deeply cleaning.
Experiments show that the acne-removing facial cleanser composition can remove acnes caused by various reasons, has the effects of resisting inflammation, relieving, moisturizing and repairing, and is suitable for various skin types. The invention discovers that on the premise of the same usage amount, the acne removing agent, the conditioner, the cleanser and the internal components thereof with specific proportions have better effects of synergy compared with the single use or the common use of any three of the acne removing agent, the conditioner, the cleanser and the internal components thereof, so that the acne removing cleansing effect is better. The invention also provides a formula for ensuring that the acne-removing facial cleanser composition has better effect on acne-removing facial cleanser, and also provides a preferable formula for applying the acne-removing facial cleanser composition to bath foam.
Detailed Description
For a better illustration of the objects, technical solutions and advantages of the present invention, the present invention is further illustrated by the following examples. It should be understood that the embodiments of the present invention are only for illustrating the technical effects of the present invention, but not for limiting the scope of the present invention, and the methods used in the embodiments are conventional methods unless specifically described otherwise.
The sources of part of raw materials in the invention are as follows:
salicylic acid: purchased from ala Ding Shiji (Shanghai) limited.
Cyclodextrin: purchased from ala Ding Shiji (Shanghai) limited.
Wild chrysanthemum extract: purchased from western ampelopsis biotechnology limited.
Dandelion extract: purchased from western ampelopsis biotechnology limited.
Paeonia root extract: purchased from western ampelopsis biotechnology limited.
Flos Matricariae Chamomillae extract: purchased from western ampelopsis biotechnology limited.
An extract of malva sylvestris: purchased from western ampelopsis biotechnology limited.
Aloe vera leaf juice: purchased from western ampelopsis biotechnology limited.
Tremella fruit body extract: purchased from western ampelopsis biotechnology limited.
Xylitol-based glucoside: purchased from ala Ding Shiji (Shanghai) limited.
Xylitol: purchased from ala Ding Shiji (Shanghai) limited.
Ammonium laureth sulfate: purchased from ala Ding Shiji (Shanghai) limited.
Cocamidopropyl betaine: purchased from ala Ding Shiji (Shanghai) limited.
Sodium lauroyl sarcosinate: purchased from ala Ding Shiji (Shanghai) limited.
Lauroyl amphoacetate sodium: purchased from ala Ding Shiji (Shanghai) limited.
Sodium cocoyl methyl taurate: purchased from ala Ding Shiji (Shanghai) limited.
Decyl glucoside: purchased from ala Ding Shiji (Shanghai) limited.
Phenoxyethanol: purchased from ala Ding Shiji (Shanghai) limited.
Sodium benzoate: purchased from ala Ding Shiji (Shanghai) limited.
Disodium EDTA: purchased from ala Ding Shiji (Shanghai) limited.
The acne removing agent disclosed by the invention comprises the following components in parts by mass as shown in table 1.
The mass parts of each component of the conditioner are shown in table 2.
The mass parts of the components of the cleaning agent are shown in table 3.
The acne-removing cleansing composition disclosed by the invention comprises the following components in parts by mass as shown in table 4.
The preparation method of the shower gel comprises the following steps:
S1, adding the composition and the chelating agent into water, heating to 80-85 ℃, stirring and cooling;
S2, after cooling to 40-45 ℃, adding the preservative and the thickener into the S1, stirring and dissolving until the temperature is less than or equal to 38 ℃, and standing for 12 hours.
The mass percentages of the specific components are shown in Table 5.
Note that: the balance is water.
Safety test
Cosmetic irritation evaluation was performed on the acne-removing cleansing body washes of examples 15-21 by using 2015 "cosmetic safety technical Specification" as a reference standard, and the test method was a skin patch test, and 70 persons with ages 16-65 were randomly distributed.
The testing method comprises the following steps: putting the test object into a patch tester with the dosage of 5g, covering the patch tester with the test object on the back or the curved side of the forearm of a subject by using a non-stimulated cloth-based adhesive tape, lightly pressing the patch tester with the palm to uniformly adhere the patch tester on the skin surface for 24 hours, removing the test object for 30 minutes, and observing skin reaction after the indentation disappears. If the result is negative, the observation is carried out again 24 hours and 48 hours after the patch test, respectively.
Evaluation criteria:
Level 0: a negative reaction;
stage 1: suspicious reactions, only weak erythema;
2 stages: weak positive response, erythema, infiltration, edema, and possibly papules;
3 stages: strong positive reaction, erythema, infiltration, edema, papule, and reaction beyond the test area;
4 stages: very strong positive response, marked erythema, severe infiltration, edema, blepharospermia, and response beyond the test area.
Test results: all subjects were negative for skin response.
The test results show that the acne-removing facial cleanser composition and the skin care product bath cream provided by the invention are mild and free of irritation to skin, and are safe to use.
Cytotoxicity test
Human corneal epithelial cells HCEC with stable growth state were taken, and the cell concentration was adjusted to 1X 10 5/mL, and inoculated into 96-well cell culture plates (except outermost peripheral wells and blank wells) at 100. Mu.L per well. 200. Mu.L of PBS was added to the outermost wells of the cell culture plates to reduce evaporation of the medium. Placing the cells in a carbon dioxide incubator, and culturing at 37+/-1 ℃ under the condition that the concentration of CO 2 is 5.0+/-1% until the cell fusion reaches more than 90%. Samples were added at a concentration of 10mg/L, and a blank, sample set, 100. Mu.L per well, was set. And (3) starting to add samples (sample groups) and PBS (blank control), keeping the time interval of adding samples in every two rows consistent, ensuring that the intervention time of each test object to cells is 5 min, sequentially sucking the test objects after 5 min, adding PBS into each hole, and cleaning the test objects for 2 times. The liquid in the hole is sucked by a vacuum suction pump or a discharge gun, and the operation is carefully performed so as not to suck out the living cells to influence the experimental result. 100. Mu.L of complete medium and 10. Mu.L of CCK-8 solution were added to each well, and the wells were placed in an incubator for cultivation for about 2 hours. At 450 nm wavelengths, absorbance (OD) values were measured and cell viability calculated; the results are shown in Table 6.
As can be seen from the results of the experiments in Table 4, the cell viability was maintained at 95% or more. The experiment collocation composition has small influence on HCEC cell viability.
Antibacterial agent for acne bacillus
Referring to WS/T650-2019, 24h fresh bevel cultures of acne bacillus were washed with PBS and diluted to 5.0X10 5CFU/mL-5.0×106 CFU/mL for use using a bacteriostatic ring test.
Preparation of antibacterial tablets: the cut diameter was 5 mm and the thickness was no more than 4 mm pieces of filter paper, each 4 pieces being a set immersed in 10mL of sample solution.
Preparation of negative control plaques: taking samples of the same material, preparing into discs (blocks) with the same size as the test group, and soaking in sterile water.
The acnes suspension with the concentration of 5.0X10 5CFU/mL-5.0×106 CFU/mL is dipped by a sterile cotton swab and evenly smeared on the surface of a proper culture medium flat plate for 3 times. And (3) each time of smearing, the flat plate rotates for 60 degrees, and finally, the cotton swab is smeared around the edge of the flat plate for one circle. The plate was covered and left to dry at room temperature for 5min. 1 bacteria-staining plate is attached to each test, and 4 test pieces, 1 negative control piece and 5 pieces are attached to each plate. The sample piece is stuck on the surface of the flat plate by using sterile forceps, the negative control sample piece is stuck on the center of the flat plate, the test sample piece is stuck on the periphery, and after the sample piece is stuck, the sample piece is lightly pressed by using the sterile forceps, so that the sample piece is clung to the surface of the flat plate. The distance between the centers of the sample pieces is more than 25 mm and the distance between the centers of the sample pieces and the periphery of the flat plate is more than 15 mm. The plate is covered, incubated at 36+ -1deg.C for 16-18 h, and the test is repeated 3 times. The diameter of the inhibition ring (including the patch) is measured by a vernier caliper and recorded, when the inhibition ring is measured, the inhibition ring which grows uniformly and completely in a sterile mode is selected for carrying out the measurement, and the diameter of the inhibition ring is measured by taking the outer edge of the inhibition ring as a boundary.
The negative control sample should not have a bacteriostasis ring, and the test sample has a bacteriostasis effect when the diameter of the bacteriostasis ring is more than 7 mm; and if the diameter of the inhibition ring is less than or equal to 7 mm, judging that the inhibition ring has no inhibition effect. And (3) judging that the test is qualified if the test has an antibacterial effect in 3 repeated tests. See table 7 for specific results.
The results show that: the comparison of the examples and the comparative examples shows that the examples are superior to the comparative examples in bacteriostasis performance, so that the preparation is carried out according to the proportion defined by the invention, and the effect achieved by the invention cannot be achieved by any default. And the default of the comparative example also proves that certain synergistic effect exists between the proportions defined by the invention.
Fourth, percutaneous Water loss (TEWL) test
The transcutaneous water loss (TEWL) is an important parameter in assessing the barrier function of the skin and is indicative of the loss of moisture in the deep dermis through evaporation of the epidermis. The higher the rate of percutaneous loss of water indicates that the more moisture is lost through the skin, the weaker the skin's ability to retain moisture.
The rate of percutaneous water loss is closely related to the hydration of the stratum corneum and is a common indicator reflecting the barrier function of the stratum corneum. In the development process of the cosmetics, the efficacy of the moisturizing cosmetics can be evaluated by testing the TEWL value of skin moisture loss. In addition, the percutaneous water loss rate can also be applied to allergic patch tests, contact dermatitis, physiotherapy, burn and monitoring of new tissues, and whether the protective function of the skin is destroyed can be found in time.
Therefore, the percutaneous water loss rate is an important parameter for evaluating skin health, and has important significance for developing and evaluating cosmetics.
190 Healthy subjects between 18 and 60 years old, with intact persons on the inner side of the arm, were selected for grouping, 10 persons in each group, and 5 men and 5 women in each group, respectively. Sample and blank groups (i.e., negative control group: no test sample) were set, and the test areas were randomly distributed in the area 1cm 2 inside the arm of the volunteer. One area was used with 1g of test product and the other area was blank, as a negative control area, and before the use of the product (T0), 2 hours after the use of the product (T1), the forearm skin percutaneous water loss rate (TEWL) was collected, and the moisturizing effect of the product was evaluated by using the variation difference of the skin index of the product treatment area and the negative control area before and after the use of the product, and the average value was taken, to obtain the following results in table 8.
The experiments show that the shower gel prepared by the application examples 15-21 has good moisturizing effect on skin compared with the shower gel prepared by the comparative examples 23-34, can obviously increase the moisture content of skin horny layer and reduce the moisture loss of skin outside, and fully proves that the shower gel disclosed by the invention scientifically customizes a compound formula aiming at the moisturizing requirement of each layer of skin epidermis, so that the full-effect moisturizing care of reducing the percutaneous water loss rate, promoting the generation of hyaluronic acid, enhancing the internal cell water circulation, strengthening the surface water locking barrier and the like is achieved.
Fifthly, human body sensory test
1. Foam Properties
Reference GB/T13173-91, measured using the Ross-Miles method. 10g of the samples of examples 15-21 and comparative examples 23-34 were weighed, dissolved in 150mg/kg of hard water and fixed to a 1000mL volumetric flask, and the prepared solution was placed in a 40℃constant temperature water bath for 0.5h. After 200mL of the test solution was flowed from a height of 90cm to the surface of 50mL of the same test solution at the bottom of the graduated cylinder at 40℃and the liquid collided to generate foam, a stopwatch was immediately started and the corresponding foam height of the system at 30s and 5min was recorded. The foaming capacity is corresponding to the foam height (H α) corresponding to 30s, and the stability of the foam is corresponding to the ratio (H β/Hα) of the corresponding foam height (H β) to H α after 5 min. See table 9 for specific test data.
2. Usage sense test
Aiming at the use performances of mildness, moistening, cleanliness, itching relieving and the like of the shower gel, the effect evaluation is carried out after 10 volunteers use the shower gel, the scoring standard adopts 10 minutes, the higher the score is, the better the effect is, and the statistical result is averaged. See table 9 for specific test data.
The results show that: comparison of examples with comparative examples shows that the examples are superior to the comparative examples in terms of foam properties, and are significantly superior to the comparative examples in terms of foam height or foam stability, and thus the preparation at the ratios defined in the present invention does not achieve the effects achieved by the present invention by any default. And the default of the comparative example also proves that certain synergistic effect exists between the proportions defined by the invention.
The results of the examples and the comparative examples show that the examples are superior to the comparative examples in terms of mildness, moisturization, cleanliness and the presence or absence of skin itch, so that the effect achieved by the present invention cannot be achieved by any default when the preparation is carried out in the proportions defined by the present invention. And the default of the comparative example also proves that certain synergistic effect exists between the proportions defined by the invention.
The embodiments described above are some, but not all embodiments of the application. The detailed description of the embodiments of the application is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

Claims (4)

1. An acne-removing cleansing composition is characterized in that: the composition comprises the following components in parts by mass: 0.5-1.5 parts of acne removing agent; 0.1-1 part of conditioner; 24-30 parts of a cleaning agent; wherein,
The acne removing agent comprises the following components in parts by mass:
5.5-7.5 parts of salicylic acid;
0.1-1 part of cyclodextrin;
0.5-1 part of wild chrysanthemum extract;
0.5-1 part of dandelion extract;
0.5-1 part of paeonia lactiflora root extract;
The conditioner comprises the following components in parts by mass:
0.1-1 part of chamomile extract;
0.1-1 part of Malva sylvestris extract;
1.5-2 parts of aloe vera leaf juice;
3-4 parts of silver ear fruiting body extract;
0.04-0.05 part of xylitol-based glucoside;
0.03-0.05 part of xylitol;
The cleaning agent comprises the following components in parts by mass:
10-11 parts of laureth ammonium sulfate;
1-1.1 parts of cocamidopropyl betaine;
0.8-1 part of sodium lauroyl sarcosinate;
0.94-0.97 parts of lauroyl amphoacetate sodium;
0.3-0.4 part of cocoyl methyl taurine sodium;
0.5-0.7 part of decyl glucoside.
2. An acne-removing cleansing body wash characterized in that it comprises 20-45wt% of the composition according to claim 1.
3. The body wash of claim 2, further comprising 0.7wt% to 0.9wt% of a preservative, wherein the preservative is at least one of phenoxyethanol and sodium benzoate; 2-4wt% of a thickening agent, wherein the thickening agent is sodium chloride; 0.5-1.5 wt% of chelating agent, wherein the chelating agent is disodium EDTA.
4. A body wash according to claim 3, characterized in that the preparation process is as follows:
S1, adding a cleaning agent and a chelating agent into water, heating to 80-85 ℃, stirring, and cooling;
S2, adding the acne removing agent, the conditioner, the preservative and the thickener into the S1 after cooling to 40-45 ℃, stirring and dissolving until the temperature is less than or equal to 38 ℃, and standing for 12 hours.
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CN107789278A (en) * 2017-10-31 2018-03-13 思拓科(上海)生物科技股份有限公司 Organic baby bath dew
CN109730954A (en) * 2019-02-23 2019-05-10 中美杜克(广州)护肤研究中心(普通合伙) It is quick with town, releive, maintenance, the skin care item and preparation method thereof for improving sebum film effect

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