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CN114747652A - A kind of peanut protein pie rich in dietary fiber and preparation method thereof - Google Patents

A kind of peanut protein pie rich in dietary fiber and preparation method thereof Download PDF

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Publication number
CN114747652A
CN114747652A CN202210485567.7A CN202210485567A CN114747652A CN 114747652 A CN114747652 A CN 114747652A CN 202210485567 A CN202210485567 A CN 202210485567A CN 114747652 A CN114747652 A CN 114747652A
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peanut protein
peanut
powder
protein
rich
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CN114747652B (en
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孙玉鼎
贾利蓉
朱美淋
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Shandong Juren Jianbang Food Technology Co ltd
Rushan Jinguo Food Co ltd
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Shandong Juren Jianbang Food Technology Co ltd
Rushan Jinguo Food Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins
    • A23J3/16Vegetable proteins from soybean
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/30Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/346Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
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  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Seeds, Soups, And Other Foods (AREA)

Abstract

The invention discloses a peanut protein pie rich in dietary fibers and a preparation method thereof, and relates to the technical field of food processing. The peanut protein pie rich in dietary fiber comprises the following raw materials in parts by weight: 10-15 parts of peanut protein powder, 5-7 parts of soybean protein isolate, 45-50 parts of water, 10-15 parts of vegetable oil, 1-3 parts of linseed powder, 0.3-1 part of transglutaminase and 7-9 parts of oat. The peanut protein pie rich in dietary fibers adopts peanut protein powder as a main raw material, has no beany flavor and is fine and smooth in taste; the peanut protein has good water retention, is beneficial to reducing the water addition amount and reduces the manufacturing cost of ice water. Furthermore, the invention balances the amino acid defects of peanut protein by adding the isolated soy protein powder, the oat and the flaxseed powder, improves the gel performance and the product texture, has simple and clean labeling and no beany flavor, and endows the peanut with fresh and sweet taste and the oat with chewing feeling.

Description

一种富含膳食纤维的花生蛋白派及其制备方法A kind of peanut protein pie rich in dietary fiber and preparation method thereof

技术领域technical field

本发明涉及食品加工技术领域,具体而言,涉及一种富含膳食纤维的花生蛋白派及其制备方法。The invention relates to the technical field of food processing, in particular to a peanut protein pie rich in dietary fiber and a preparation method thereof.

背景技术Background technique

现有的蛋白类凝胶产品以大豆分离蛋白为主要成分,但由于大豆蛋白有天然的豆腥味,许多消费者难以接受,故产品需要进行卤制等工艺以去腥,此环节耗能耗费高;其次,加入调味料使产品标签复杂化,不符合绿色健康食品大趋势。此外,现存的花生蛋白干采用双螺旋高水分设备进行加工,设备的采购和维修费用昂贵,增加了生产成本和难度,且生产的凝胶产品硬度大、细腻感不强。因此,为了避免增加冗杂的去腥工艺、设备采购及维修费用,故对蛋白凝胶产品配方和工艺进行调整。Existing protein gel products use soybean protein isolate as the main component, but because soybean protein has a natural beany smell, which is unacceptable to many consumers, the product needs to undergo processes such as halogenation to remove the fishy smell, which consumes a lot of energy. ; Second, adding seasonings complicates product labels and does not conform to the general trend of green and healthy food. In addition, the existing dried peanut protein is processed by double-helix high-moisture equipment, which is expensive to purchase and maintain, which increases the production cost and difficulty, and produces a gel product with high hardness and low delicate feel. Therefore, in order to avoid increasing the complicated deodorization process, equipment procurement and maintenance costs, the protein gel product formulation and process were adjusted.

鉴于此,特提出本发明。In view of this, the present invention is proposed.

发明内容SUMMARY OF THE INVENTION

本发明的目的在于提供富含膳食纤维的花生蛋白派和富含膳食纤维的花生蛋白派的制备方法。An object of the present invention is to provide a dietary fiber-rich peanut protein pie and a method for preparing the dietary fiber-rich peanut protein pie.

本发明是这样实现的:The present invention is realized in this way:

第一方面,本发明提供一种富含膳食纤维的花生蛋白派,其原料按重量份数计包括:花生蛋白粉10-15份、大豆分离蛋白5-7份、水45-50份、植物油10-15份、亚麻籽粉1-3份、谷氨酰胺转胺酶0.3-1份和燕麦7-9份。In the first aspect, the present invention provides a peanut protein pie rich in dietary fiber, the raw materials of which, in parts by weight, include: 10-15 parts of peanut protein powder, 5-7 parts of soybean protein isolate, 45-50 parts of water, and vegetable oil. 10-15 parts, 1-3 parts flaxseed meal, 0.3-1 part transglutaminase and 7-9 parts oat.

在可选的实施方式中,所述花生蛋白粉和所述大豆分离蛋白的质量比为1.5-2.5:1。In an optional embodiment, the mass ratio of the peanut protein powder and the soybean protein isolate is 1.5-2.5:1.

在可选的实施方式中,所述水的加入量占所述花生蛋白粉、所述大豆分离蛋白和所述水的总质量的72-74%,优选为73%。In an optional embodiment, the added amount of the water accounts for 72-74%, preferably 73%, of the total mass of the peanut protein powder, the soybean protein isolate and the water.

在可选的实施方式中,所述亚麻籽粉的加入量占所述花生蛋白粉、所述大豆分离蛋白、所述水、所述植物油和所述亚麻籽粉的总质量的2-5%,优选为3%。In an optional embodiment, the added amount of the flaxseed meal accounts for 2-5% of the total mass of the peanut protein meal, the soy protein isolate, the water, the vegetable oil and the flaxseed meal , preferably 3%.

所述燕麦为干燕麦经过沸水浸泡20-40min后沥干后制得的,所述燕麦与所述干燕麦的质量比为7-9:5。The oat is prepared by soaking dry oat in boiling water for 20-40 min and then draining, and the mass ratio of the oat to the dry oat is 7-9:5.

在可选的实施方式中,所述干燕麦的加入量占所述花生蛋白粉、所述大豆分离蛋白、所述水、所述植物油、所述亚麻籽粉和所述谷氨酰胺转胺酶的总质量的5-7%,优选为6%。In an optional embodiment, the dry oat is added in an amount equal to the amount of the peanut protein powder, the soy protein isolate, the water, the vegetable oil, the flaxseed meal and the transglutaminase. 5-7% of the total mass, preferably 6%.

在可选的实施方式中,所述植物油为花生油、大豆油、菜籽油、芝麻油、棉籽油、米糠油、玉米油、葵花籽油和亚麻油中的至少一种;优选地,所述植物油为花生油。In an optional embodiment, the vegetable oil is at least one of peanut oil, soybean oil, rapeseed oil, sesame oil, cottonseed oil, rice bran oil, corn oil, sunflower oil and linseed oil; preferably, the vegetable oil for peanut oil.

第二方面,本发明提供一种富含膳食纤维的花生蛋白派的制备方法,其包括将如前述实施方式任一项所述的富含膳食纤维的花生蛋白派的原料混合得到斩拌物料,所述斩拌物料经入模加热,脱模烘烤制得。In a second aspect, the present invention provides a method for preparing a peanut protein pie rich in dietary fiber, which comprises mixing the raw materials of the peanut protein pie rich in dietary fiber according to any one of the foregoing embodiments to obtain a chopping material, The chopped and mixed material is heated in a mold, and then demolded and baked.

在可选的实施方式中,将所述原料混合包括:先将所述花生蛋白粉、所述大豆分离蛋白和所述水于3000-3500r/min的搅拌速度下斩拌均匀,于3000-3500r/min的搅拌速度斩拌4-6min;接着加入所述植物油,于3000-3500r/min的搅拌速度斩拌2-4min;接着加入所述亚麻籽粉、所述谷氨酰胺转胺酶和所述燕麦,继续于3000-3500r/min的搅拌速度斩拌1-2min,得到斩拌物料;In an optional embodiment, mixing the raw materials includes: firstly chopping the peanut protein powder, the soybean protein isolate and the water evenly at a stirring speed of 3000-3500r/min; The stirring speed of /min is chopped and mixed for 4-6min; then the vegetable oil is added, and the stirring speed of 3000-3500r/min is chopped and mixed for 2-4min; then the flaxseed powder, the transglutaminase and the Describe oat, continue to chop and mix 1-2min at the stirring speed of 3000-3500r/min, obtain chopped material;

优选地,所述入模加热包括:将所述斩拌物料置于模具中于54-56℃环境中放置1-2h;Preferably, the heating into the mold comprises: placing the chopped material in the mold for 1-2 hours in an environment of 54-56°C;

优选地,所述脱模烘烤包括:将从所述模具中脱模后的料坯进行切块,并于60-80℃、烘烤时间为10-20min;Preferably, the demoulding baking includes: dicing the blank after demoulding from the mold, and baking at 60-80° C. for 10-20 min;

优选地,在烘烤期间对所述坯料进行翻面1-2次。Preferably, the blanks are turned 1-2 times during baking.

在可选的实施方式中,在所述脱模烘烤之后,还包括包装灭菌步骤:将烘烤结束后产品冷却至室温,抽真空后于温度为110-120℃,压力为0.15-0.2MPa的条件下灭菌25-35min。In an optional embodiment, after the demoulding and baking, a packaging sterilization step is also included: cooling the product after baking to room temperature, vacuuming the product at a temperature of 110-120° C. and a pressure of 0.15-0.2 Sterilize under the condition of MPa for 25-35min.

本发明具有以下有益效果:The present invention has the following beneficial effects:

本申请提供的富含膳食纤维的花生蛋白派采用花生蛋白粉为主要原料,较传统蛋白凝胶制品,本发明无豆腥味且口感细腻,无需调味、卤制以去腥,也无需使用高水分挤压等设备,节约了能源损耗和生产成本;花生蛋白持水性良好,以花生蛋白粉为主要原料后,进一步减少了水分添加量,降低了冰水制作成本。进一步地,本发明在花生蛋白粉的基础上,添加了大豆分离蛋白粉、燕麦和亚麻籽粉。花生蛋白粉口感清甜、持水性良好,但花生蛋白的赖氨酸、苏氨酸、蛋氨酸含量较低,凝胶性较弱;大豆分离蛋白粉、燕麦和亚麻籽粉这三种辅料平衡了花生蛋白的氨基酸缺陷,三者凝胶性能均良好,提升了产品质构。同时亚麻籽粉还可以替代了变性淀粉等粘合类食品添加剂,清洁了产品标签、丰富了蛋白派的营养价值。本发明在原料斩拌后,将混合物置于55℃环境中静置1h即可达到良好的硬度和弹性,其他蛋白类凝胶产品至少需要反应2h,多则需要10h以上。因此,本发明增强了工艺连续性、节约了能源损耗,提升了生产速度。The peanut protein pie rich in dietary fiber provided by the present application uses peanut protein powder as the main raw material. Compared with traditional protein gel products, the present invention has no beany smell and has a finer mouthfeel, no need for seasoning, marinating to remove fishy smell, and no need to use high water content. Extrusion and other equipment saves energy loss and production costs; peanut protein has good water holding capacity, and using peanut protein powder as the main raw material further reduces the amount of water added and the cost of making ice water. Further, the present invention adds soybean protein isolate powder, oat and flaxseed powder on the basis of peanut protein powder. Peanut protein powder has a sweet taste and good water retention, but the content of lysine, threonine, and methionine in peanut protein is low, and the gelling property is weak; soy protein isolate powder, oat and flaxseed powder are balanced by three accessories Due to the amino acid deficiency of peanut protein, the gel properties of the three are good, which improves the product texture. At the same time, flaxseed meal can also replace adhesive food additives such as modified starch, which cleans product labels and enriches the nutritional value of protein pie. In the present invention, after the raw materials are chopped and stirred, the mixture is placed in a 55°C environment for 1 hour to achieve good hardness and elasticity. Other protein gel products require at least 2 hours of reaction, and more than 10 hours. Therefore, the present invention enhances process continuity, saves energy consumption, and increases production speed.

附图说明Description of drawings

为了更清楚地说明本发明实施例的技术方案,下面将对实施例中所需要使用的附图作简单地介绍,应当理解,以下附图仅示出了本发明的某些实施例,因此不应被看作是对范围的限定,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他相关的附图。In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the following briefly introduces the accompanying drawings used in the embodiments. It should be understood that the following drawings only show some embodiments of the present invention, and therefore do not It should be regarded as a limitation of the scope, and for those of ordinary skill in the art, other related drawings can also be obtained according to these drawings without any creative effort.

图1为本申请试验例1提供的蛋白粉添加比例对蛋白派感官品质的影响柱状评分图;Fig. 1 is a bar chart of the influence of the protein powder addition ratio on the sensory quality of protein pie provided by Test Example 1 of this application;

图2为本申请试验例2提供的冰水添加量对蛋白派感官品质的影响柱状评分图;Fig. 2 is a bar chart of the influence of the addition of ice water on the sensory quality of protein pie provided by Test Example 2 of this application;

图3为本申请试验例3提供的亚麻籽粉添加量对蛋白派感官品质的影响柱状评分图;Fig. 3 is a bar chart of the influence of the addition of flaxseed powder on the sensory quality of protein pie provided by Test Example 3 of the present application;

图4为本申请试验例3提供的亚麻籽粉与变形淀粉作为粘合剂时对蛋白派感官品质的影响柱状评分图;Fig. 4 is a bar score chart of the influence on the sensory quality of protein pie when the flaxseed meal and deformed starch provided by Test Example 3 of the application are used as adhesives;

图5为本申请试验例4提供的谷氨酰胺转胺酶反应条件对蛋白派感官品质的影响柱状评分图;Fig. 5 is a bar chart of the influence of the transglutaminase reaction conditions provided by Test Example 4 of the application on the sensory quality of protein pie;

图6为本申请试验例5提供的燕麦添加量对蛋白派感官品质的影响柱状评分图;Fig. 6 is a bar chart of the influence of the oat addition provided by Test Example 5 of the application on the sensory quality of protein pie;

图7为本申请试验例6提供的燕麦添加量对蛋白派感官品质的影响柱状评分图;Fig. 7 is a bar chart of the influence of the addition of oats on the sensory quality of protein pie provided by Test Example 6 of the present application;

图8为本申请试验例6提供的烘烤时间对蛋白派感官品质的影响柱状评分图。FIG. 8 is a bar score chart of the influence of baking time on the sensory quality of the meringue provided by Test Example 6 of the present application.

具体实施方式Detailed ways

为使本发明实施例的目的、技术方案和优点更加清楚,下面将对本发明实施例中的技术方案进行清楚、完整地描述。实施例中未注明具体条件者,按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者,均为可以通过市售购买获得的常规产品。In order to make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not indicated in the examples, it is carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used without the manufacturer's indication are conventional products that can be purchased from the market.

本发明提供一种富含膳食纤维的花生蛋白派,其原料按重量份数计包括:花生蛋白粉10-15份、大豆分离蛋白5-7份、水45-50份、植物油10-15份、亚麻籽粉1-3份、谷氨酰胺转胺酶0.3-1份和燕麦7-9份。The invention provides a peanut protein pie rich in dietary fiber. The raw materials, in parts by weight, include: 10-15 parts of peanut protein powder, 5-7 parts of soybean protein isolate, 45-50 parts of water, and 10-15 parts of vegetable oil , 1-3 parts of flaxseed meal, 0.3-1 part of transglutaminase and 7-9 parts of oat.

花生蛋白粉颜色洁白,具有花生特有的清香气味,较完整地保留了花生中的营养成分,富含大量的必需氨基酸、维生素、微量元素及矿物质,其抗营养因子含量较大豆蛋白少,易被人体消化吸收,是低糖、低脂肪、零胆固醇、高营养价值的天然营养品。花生蛋白凝胶性较差,故限制了其在食品凝胶类产品的应用。Peanut protein powder is white in color and has the unique fragrance of peanuts. It completely retains the nutrients in peanuts, and is rich in a large amount of essential amino acids, vitamins, trace elements and minerals. Its anti-nutritional factor content is higher than that of soybean protein. Digested and absorbed by the human body, it is a natural nutritional product with low sugar, low fat, zero cholesterol and high nutritional value. Peanut protein has poor gel properties, which limits its application in food gel products.

大豆蛋白是一种植物性蛋白质,其氨基酸组成与牛奶蛋白质相近,是植物性的完全蛋白质。研究表明每天摄入25g大豆蛋白,能减小患心脑血管疾病的风险。由于大豆分离蛋白中赖氨酸含量较高且产品胶凝性良好,所以将其加入到食品中,可以平衡氨基酸含量和提升产品质构感。Soy protein is a vegetable protein, its amino acid composition is similar to that of milk protein, and it is a complete vegetable protein. Studies have shown that daily intake of 25g of soy protein can reduce the risk of cardiovascular and cerebrovascular diseases. Due to the high content of lysine in soybean protein isolate and the good gelation of the product, adding it to food can balance the amino acid content and improve the texture of the product.

本申请中通过将花生蛋白粉与大豆分离蛋白进行复配,并且以花生蛋白粉作为主要原料,以大豆分离蛋白作为辅助蛋白,花生蛋白粉和大豆分离蛋白的质量比为1.5-2.5:1。较传统蛋白凝胶制品,本发明无豆腥味且口感细腻,无需调味、卤制以去腥,也无需使用高水分挤压等设备,节约了能源损耗和生产成本;花生蛋白持水性良好,以花生蛋白粉为主要原料后,进一步减少了水分添加量,降低了冰水制作成本。由于花生蛋白的赖氨酸、苏氨酸、蛋氨酸含量较低,凝胶性较弱;因此本申请通过复配胶凝性良好的大豆分离蛋白,提升了本申请的富含膳食纤维的花生蛋白派的凝胶性。In this application, the peanut protein powder is compounded with the soybean protein isolate, and the peanut protein powder is used as the main raw material, and the soybean protein isolate is used as the auxiliary protein, and the mass ratio of the peanut protein powder and the soybean protein isolate is 1.5-2.5:1. Compared with traditional protein gel products, the present invention has no beany smell and delicate taste, no need for seasoning, marinating to remove fishy smell, and no need to use equipment such as high-moisture extrusion, which saves energy loss and production cost; After peanut protein powder is used as the main raw material, the amount of water added is further reduced, and the production cost of ice water is reduced. Because the content of lysine, threonine and methionine in peanut protein is low, the gelling property is weak; therefore, the present application improves the dietary fiber-rich peanut protein of the present application by compounding soybean protein isolate with good gelling property. Pie gel properties.

水用于实现对原料进行混合,并且形成流动性较好的斩拌物料,以便入模。本申请中的水为冰水,冰:水=3:7,水的温度为4℃,冰块高速斩碎为冰沙,两者按照比例混合后加入斩拌锅。水的加入量占花生蛋白粉、大豆分离蛋白和水的总质量的72-74%,优选为73%。Water is used to realize the mixing of the raw materials, and to form a chopping material with better fluidity for injection into the mold. The water in this application is ice water, ice:water=3:7, the temperature of the water is 4°C, the ice cubes are chopped into smoothies at high speed, and the two are mixed in proportion and then added to the chopper. The added amount of water accounts for 72-74% of the total mass of peanut protein powder, soybean protein isolate and water, preferably 73%.

植物油用于起到乳化、锁水和细腻口感的作用,本申请中,植物油为花生油、大豆油、菜籽油、芝麻油、棉籽油、米糠油、玉米油、葵花籽油和亚麻油中的至少一种;优选地,植物油为花生油。本申请中选用花生油可以进一步提升富含膳食纤维的花生蛋白派的花生味,使得花生风味更为浓郁。Vegetable oil is used for emulsifying, locking water and delicate taste. In this application, vegetable oil is at least one of peanut oil, soybean oil, rapeseed oil, sesame oil, cottonseed oil, rice bran oil, corn oil, sunflower oil and linseed oil. one; preferably, the vegetable oil is peanut oil. The selection of peanut oil in the present application can further enhance the peanut flavor of the peanut protein pie rich in dietary fiber, making the peanut flavor more intense.

亚麻籽的主要成分为脂肪、蛋白质、膳食纤维,目前,亚麻籽主要用来榨取油脂,榨油后的副产物蛋白质含量高达35%以上,其氨基酸的组成和比例符合WHO/FAO规定人体所需氨基酸模式的要求,每100g亚麻籽便含有27.3g膳食纤维,是一种优质的植物蛋白来源。研究表明一汤匙亚麻籽含有3800毫克的奥米茄三脂肪酸,相当于深海鱼油的10倍。亚麻籽粉与一定比例的水混合后可形成黏稠的液体,具有黏连的作用,本申请采用亚麻籽粉替代传统的粘合类食品添加剂,产品标签绿色清洁,丰富了蛋白派的营养价值,同时本申请采用亚麻籽粉进行凝胶,凝胶过程显著降低,有利于提升生产效率。亚麻籽粉的加入量占花生蛋白粉、大豆分离蛋白、水、植物油和亚麻籽粉的总质量的2-5%,优选为3%。The main components of flaxseed are fat, protein and dietary fiber. At present, flaxseed is mainly used to extract oil. The protein content of the by-product after oil extraction is as high as 35%. The composition and proportion of amino acids meet the requirements of WHO/FAO for human body. According to the requirements of the amino acid pattern, every 100g of flaxseed contains 27.3g of dietary fiber, which is a high-quality source of vegetable protein. Research has shown that one tablespoon of flaxseed contains 3,800 mg of omega-3 fatty acids, which is 10 times more than deep-sea fish oil. After the flaxseed powder is mixed with a certain proportion of water, it can form a viscous liquid, which has the effect of adhesion. The application uses flaxseed powder to replace the traditional adhesive food additives, and the product label is green and clean, which enriches the nutritional value of the protein pie. At the same time, the present application uses flaxseed powder for gelation, and the gelation process is significantly reduced, which is beneficial to improve production efficiency. The added amount of linseed meal accounts for 2-5%, preferably 3%, of the total mass of peanut protein powder, soybean protein isolate, water, vegetable oil and linseed meal.

谷氨酰胺转胺酶是一种可以催化转酰基反应,从而导致蛋白质(或多肽)之间发生共价交联的酶。其可以催化蛋白质之间发生交联,使蛋白质改性,改性后的蛋白质其塑性、持水性、水溶性和功能性得到改善;保护了食品中的赖氨酸免受各种化学反应的破坏。Transglutaminase is an enzyme that catalyzes the transacylation reaction, resulting in covalent cross-linking between proteins (or polypeptides). It can catalyze the cross-linking between proteins, modify the protein, and improve the plasticity, water-holding capacity, water-solubility and functionality of the modified protein; protect the lysine in food from the destruction of various chemical reactions .

燕麦被誉为天然膳食纤维家族中的“贵族”,燕麦籽粒中β-葡聚糖含量在谷类作物中最高,已达到3.0%~6.0%,美国食品和药物管理局FDA指出,每日饮食中含有3g以上β-葡聚糖或者食用低脂肪的燕麦可以降低血脂含量,并能减少心血管疾病的发病率。燕麦淀粉具有较好的凝胶特性,使食品具有润滑、致密和奶油状的结构,可改善食品的质地和口感。本申请中通过添加燕麦,不仅仅能够增强富含膳食纤维的花生蛋白派的膳食纤维含量,同时还可以提升产品的质地和口感。本申请中,燕麦为干燕麦经过沸水浸泡20-40min后沥干后制得的,燕麦与干燕麦的质量比为7-9:5。干燕麦的加入量占花生蛋白粉、大豆分离蛋白、水、植物油、亚麻籽粉和谷氨酰胺转胺酶的总质量的5-7%,优选为6%。Oats are known as the "noble" in the natural dietary fiber family. The content of β-glucan in oat grains is the highest among cereal crops, reaching 3.0% to 6.0%. Containing more than 3g of beta-glucan or eating low-fat oats can reduce blood lipid content and reduce the incidence of cardiovascular disease. Oat starch has good gel properties, which give food a smooth, dense and creamy structure, which can improve the texture and taste of food. In the present application, by adding oatmeal, not only the dietary fiber content of the peanut protein pie rich in dietary fiber can be enhanced, but also the texture and taste of the product can be improved. In the present application, the oat is obtained by soaking dry oat in boiling water for 20-40min and then draining, and the mass ratio of oat and dry oat is 7-9:5. The added amount of dry oats accounts for 5-7%, preferably 6%, of the total mass of peanut protein powder, soybean protein isolate, water, vegetable oil, flaxseed meal and transglutaminase.

此外,本发明还提供了上述富含膳食纤维的花生蛋白派的制备方法,其包括将上述富含膳食纤维的花生蛋白派的原料混合得到斩拌物料,斩拌物料经入模加热,脱模烘烤制得。In addition, the present invention also provides a method for preparing the above-mentioned dietary fiber-rich peanut protein pie, which comprises mixing the raw materials of the above-mentioned dietary fiber-rich peanut protein pie to obtain a chopping material, and the chopping material is heated in a mold, and then demolded. Made by baking.

具体来说,包括如下步骤:Specifically, it includes the following steps:

S1、原料混合:先将花生蛋白粉、大豆分离蛋白和水于3000-3500r/min的搅拌速度下斩拌均匀,于3000-3500r/min的搅拌速度斩拌4-6min;接着加入植物油,于3000-3500r/min的搅拌速度斩拌2-4min;接着加入亚麻籽粉、谷氨酰胺转胺酶和燕麦,继续于3000-3500r/min的搅拌速度斩拌1-2min,得到斩拌物料;S1. Mixing of raw materials: firstly chop and mix peanut protein powder, soybean protein isolate and water at a stirring speed of 3000-3500r/min, then chop and mix for 4-6min at a stirring speed of 3000-3500r/min; then add vegetable oil, Stirring at a stirring speed of 3000-3500r/min for 2-4min; then adding flaxseed powder, transglutaminase and oats, and continuing to stir at a stirring speed of 3000-3500r/min for 1-2min, to obtain a chopping material;

S2、入模加热包括:将斩拌物料置于模具中于54-56℃环境中放置1-2h;S2. Heating into the mold includes: placing the chopped material in the mold for 1-2h in an environment of 54-56°C;

S3、脱模烘烤包括:将从模具中脱模后的料坯进行切块,并于60-80℃、烘烤时间为10-20min;在烘烤期间对切块坯料进行翻面1-2次。S3, demoulding baking includes: dicing the blank after demoulding from the mold, and baking at 60-80°C for 10-20 min; turning the diced blank during the baking 1- 2 times.

S4、包装灭菌步骤:将烘烤结束后产品冷却至室温,抽真空后于温度为110-120℃,压力为0.15-0.2MPa的条件下灭菌25-35min。S4, packaging sterilization step: cool the product to room temperature after baking, vacuumize the product, and sterilize it for 25-35 minutes at a temperature of 110-120°C and a pressure of 0.15-0.2MPa.

以下结合实施例对本发明的特征和性能作进一步的详细描述。The features and performances of the present invention will be further described in detail below in conjunction with the embodiments.

实施例1Example 1

本实施例提供了一种富含膳食纤维的花生蛋白派,其制备方法包括如下步骤:The present embodiment provides a kind of peanut protein pie rich in dietary fiber, and its preparation method comprises the following steps:

S1、原料斩拌入模:S1. The raw materials are chopped and mixed into the mold:

原料斩拌分为三个步骤:第一步将12kg花生蛋白粉、6kg大豆分离蛋白、48kg冰水混合物放入斩拌锅中低速斩拌至混合均匀后,于3200r/min的搅拌速度下斩拌5min;第二步加入12kg花生油,于3200r/min的搅拌速度下斩拌3min;第三步加入2.4kg亚麻籽粉(亚麻籽高速粉碎为粉)、0.6kg谷氨酰胺转胺酶和8kg燕麦(约5kg干燕麦经沸水浸泡30min后沥水),于3200r/min的搅拌速度下斩拌1min,最终得到斩拌物料并入模;The chopping and mixing of raw materials is divided into three steps: the first step is to put 12 kg of peanut protein powder, 6 kg of soybean protein isolate, and 48 kg of ice-water mixture into the chopping pot at a low speed and stir until the mixing is uniform, then chop at a stirring speed of 3200 r/min. Mix for 5min; add 12kg peanut oil in the second step, chop and mix for 3min at a stirring speed of 3200r/min; add 2.4kg flaxseed powder (the flaxseed is ground into powder at high speed), 0.6kg transglutaminase and 8kg in the third step Oat (about 5kg dry oat soaked in boiling water for 30min and drained), chopped and mixed for 1min at a stirring speed of 3200r/min, finally obtained the chopped material and incorporated into the mold;

S2、入模加热:S2, mold heating:

将带斩拌物料的模具于55℃环境中放置1h;Place the mold with chopped material at 55°C for 1 hour;

S3、脱模烘烤:S3, demoulding baking:

将混合物从模具中取出,按照长*宽*高为6*4*1.5的比例切块成条,于70℃环境中烘烤20min,在10min、18min时将产品翻面后继续烘烤。Take the mixture out of the mold, cut it into strips according to the ratio of length*width*height of 6*4*1.5, bake at 70℃ for 20min, turn the product over at 10min and 18min and continue to bake.

S4、包装灭菌:S4, packaging sterilization:

烘烤结束后冷却至室温,抽真空后在温度为115℃,压力为0.18MPa的灭菌锅中灭菌30min。After baking, cool to room temperature, vacuumize and sterilize in a sterilizer with a temperature of 115°C and a pressure of 0.18MPa for 30min.

实施例2-3Example 2-3

实施例2-3与实施例1基本相同,区别仅在于,蛋白粉的比例不同:Examples 2-3 are basically the same as Example 1, the only difference being that the proportions of protein powder are different:

实施例2中,蛋白粉包括14.4kg花生蛋白粉和7.2kg大豆分离蛋白(即花生蛋白粉:大豆分离蛋白=1.5:1);In Example 2, the protein powder includes 14.4 kg peanut protein powder and 7.2 kg soybean protein isolate (ie, peanut protein powder: soybean protein isolate=1.5:1);

实施例3中,蛋白粉包括12.9kg花生蛋白粉和5.1kg大豆分离蛋白。In Example 3, the protein powder included 12.9 kg of peanut protein powder and 5.1 kg of soy protein isolate.

实施例4Example 4

本实施例提供了一种富含膳食纤维的花生蛋白派,其制备方法包括如下步骤:The present embodiment provides a kind of peanut protein pie rich in dietary fiber, and its preparation method comprises the following steps:

S1、原料斩拌入模:S1. The raw materials are chopped and mixed into the mold:

原料斩拌分为三个步骤:第一步将10kg花生蛋白粉、5kg大豆分离蛋白、45kg冰水混合物放入斩拌锅中于3200r/min的搅拌速度下斩拌至混合均匀后,于3200r/min的搅拌速度下斩拌4min;第二步加入10kg花生油,于3200r/min的搅拌速度下斩拌2min;第三步加入1.4kg亚麻籽粉(亚麻籽高速粉碎为粉)、0.4kg谷氨酰胺转胺酶和7kg燕麦(约4kg干燕麦经沸水浸泡30min后沥水),于3200r/min的搅拌速度下斩拌1min,最终得到斩拌物料并入模;The chopping and mixing of raw materials is divided into three steps: the first step is to put 10kg of peanut protein powder, 5kg of soybean protein isolate, and 45kg of ice-water mixture into the chopping pot and chop and mix at a stirring speed of 3200r/min until they are evenly mixed. Chopping and mixing 4min at the stirring speed of /min; adding 10kg peanut oil in the second step, chopping and mixing 2min under the stirring speed of 3200r/min; adding 1.4kg flaxseed powder (the flaxseed is crushed into powder at high speed), 0.4kg grain in the third step Amino aminotransferase and 7kg oats (about 4kg dry oats are soaked in boiling water for 30min and drained), chopped and mixed for 1min at a stirring speed of 3200r/min, and finally obtained the chopped material and incorporated into the mold;

S2、入模加热:S2, mold heating:

将带斩拌物料的模具于54℃环境中放置2h;Place the mold with chopped material at 54°C for 2h;

S3、脱模烘烤:S3, demoulding baking:

将混合物从模具中取出,按照长*宽*高为6*4*1.5的比例切块成条,于80℃环境中烘烤15min,在7min、12min时将产品翻面后继续烘烤。Take the mixture out of the mold, cut it into strips according to the ratio of length*width*height of 6*4*1.5, bake at 80°C for 15min, turn the product over at 7min and 12min and continue to bake.

S4、包装灭菌:S4, packaging sterilization:

烘烤结束后冷却至室温,抽真空后在温度为110℃,压力为0.2MPa的灭菌锅中灭菌35min。After baking, it was cooled to room temperature, and after vacuuming, it was sterilized in a sterilizing pot with a temperature of 110° C. and a pressure of 0.2 MPa for 35 minutes.

实施例5Example 5

本实施例提供了一种富含膳食纤维的花生蛋白派,其制备方法包括如下步骤:The present embodiment provides a kind of peanut protein pie rich in dietary fiber, and its preparation method comprises the following steps:

S1、原料斩拌入模:S1. The raw materials are chopped and mixed into the mold:

原料斩拌分为三个步骤:第一步将15kg花生蛋白粉、7kg大豆分离蛋白、50kg冰水混合物放入斩拌锅中于3200r/min的搅拌速度下斩拌至混合均匀后,于3200r/min的搅拌速度下斩拌6min;第二步加入15kg花生油,于3200r/min的搅拌速度下斩拌4min;第三步加入3kg亚麻籽粉(亚麻籽高速粉碎为粉)、0.8kg谷氨酰胺转胺酶和9kg燕麦(约6kg干燕麦经沸水浸泡30min后沥水),于3200r/min的搅拌速度下斩拌1min,最终得到斩拌物料并入模;The chopping and mixing of raw materials is divided into three steps: the first step is to put 15kg of peanut protein powder, 7kg of soybean protein isolate, and 50kg of ice-water mixture into a chopping pot and chop and mix at a stirring speed of 3200r/min until they are evenly mixed. Chop and mix 6min at the stirring speed of /min; add 15kg peanut oil in the second step, chop and mix for 4min at the stirring speed of 3200r/min; add 3kg flaxseed powder (the flaxseed is ground into powder at high speed), 0.8kg glutamine in the third step Amidotransferase and 9kg of oats (about 6kg of dry oats are soaked in boiling water for 30min and drained), chopped and mixed for 1min at a stirring speed of 3200r/min, and finally obtained the chopped material and incorporated into the mold;

S2、入模加热:S2, mold heating:

将带斩拌物料的模具于56℃环境中放置1h;Place the mold with chopped material at 56°C for 1 hour;

S3、脱模烘烤:S3, demoulding baking:

将混合物从模具中取出,按照长*宽*高为6*4*1.5的比例切块成条,于60℃环境中烘烤20min,在10min、18min时将产品翻面后继续烘烤。Take the mixture out of the mold, cut it into strips according to the ratio of length*width*height 6*4*1.5, bake at 60°C for 20min, turn the product over at 10min and 18min and continue to bake.

S4、包装灭菌:S4, packaging sterilization:

烘烤结束后冷却至室温,抽真空后在温度为120℃,压力为0.15MPa的灭菌锅中灭菌25min。After baking, cool to room temperature, vacuumize and sterilize in a sterilizer with a temperature of 120°C and a pressure of 0.15MPa for 25min.

对比例1-5Comparative Examples 1-5

对比例1-5与实施例1基本相同,区别仅在于,蛋白粉的选择和比例不同,实施例1中蛋白粉包括12kg花生蛋白粉和6kg大豆分离蛋白;Comparative Examples 1-5 are basically the same as Example 1, except that the selection and ratio of protein powder are different. In Example 1, the protein powder includes 12kg peanut protein powder and 6kg soybean protein isolate;

对比例1中,蛋白粉包括10.5kg花生蛋白粉、4.5kg大豆分离蛋白和3kg谷朊粉(即花生蛋白粉:大豆分离蛋白:谷朊粉=7:3:2)。In Comparative Example 1, the protein powder included 10.5 kg of peanut protein powder, 4.5 kg of soybean protein isolate and 3 kg of gluten powder (ie, peanut protein powder: soybean protein isolate: gluten powder = 7:3:2).

对比例2中,蛋白粉包括10.8kg花生蛋白粉、3.6kg大豆分离蛋白和3.6kg谷朊粉(即花生蛋白粉:大豆分离蛋白:谷朊粉=6:2:2)。In Comparative Example 2, the protein powder included 10.8 kg of peanut protein powder, 3.6 kg of soybean protein isolate and 3.6 kg of gluten (ie, peanut protein powder: soybean protein isolate: gluten = 6:2:2).

对比例3中,蛋白粉包括10.8kg花生蛋白粉、5.4kg大豆分离蛋白和1.8kg谷朊粉(即花生蛋白粉:大豆分离蛋白:谷朊粉=6:3:1)。In Comparative Example 3, the protein powder included 10.8 kg of peanut protein powder, 5.4 kg of soybean protein isolate and 1.8 kg of gluten (ie, peanut protein powder: soybean protein isolate: gluten = 6:3:1).

对比例4中,蛋白粉包括9kg花生蛋白粉、7.2kg大豆分离蛋白和1.8kg谷朊粉(即花生蛋白粉:大豆分离蛋白:谷朊粉=5:4:1)。In Comparative Example 4, the protein powder included 9 kg of peanut protein powder, 7.2 kg of soybean protein isolate and 1.8 kg of gluten powder (ie, peanut protein powder: soybean protein isolate: gluten powder = 5:4:1).

对比例5中,蛋白粉包括3.6kg花生蛋白粉和14.4kg大豆分离蛋白(即花生蛋白粉:大豆分离蛋白=1:4)。In Comparative Example 5, the protein powder included 3.6 kg of peanut protein powder and 14.4 kg of soy protein isolate (ie, peanut protein powder: soy protein isolate=1:4).

试验例Test example

本申请通过对各个实施例和对比例进行感官评分,通过最终得分的高低来评价各个实施例和对比例获得的花生蛋白派的优劣情况,感官评分标准请参阅表1,每个试验选择10名具有良好感官判断能力和食品相关专业背景试验人员分别对下述产品进行感官评定,评价过程中禁止相互讨论。重复试验3次,取平均值。In this application, the sensory scoring of each example and comparative example is carried out, and the pros and cons of the peanut protein pie obtained by each example and comparative example are evaluated by the final score. Please refer to Table 1 for sensory scoring standards, and select 10 for each test. Testers with good sensory judgment ability and food-related professional background test personnel respectively conduct sensory evaluation of the following products, and mutual discussion is prohibited during the evaluation process. Repeat the test 3 times and take the average value.

表1花生蛋白派感官评分标准Table 1 Sensory evaluation criteria of peanut protein pie

Figure BDA0003629052010000101
Figure BDA0003629052010000101

1、蛋白粉比例试验1. Protein powder ratio test

将实施例1以及对比例1-5提供的产品按照如表1所示的花生蛋白派感官评分标准进行评分,实验结果如图1所示,当花生蛋白粉添加过多、大豆蛋白粉过少时蛋白派质构松散,弹性和硬度弱;花生蛋白粉:大豆蛋白粉=6:3时,蛋白派质构和口感良好;没有添加谷朊粉时,产品的弹性增加,风味更加清甜。因此,舍去谷朊粉,确定花生蛋白粉和大豆蛋白粉的最佳比例,花生蛋白:大豆蛋白=6:3。此外,从对比例5的数据可以看出,当大豆蛋白粉的用量大于花生蛋白粉的用量时,此时,弹性和硬度适中,豆腥味较重,需要后续去腥,加大了工艺复杂度,感官评分下降。充分证明,按照本申请的花生蛋白粉和大豆蛋白粉1.5-2.5:1可以获得产品质量佳,感官评分高的产品,当超出该范围或者选用其他蛋白粉与花生蛋白粉进行配合时,无法获得本申请的产品质量和感官评分。The products provided by Example 1 and Comparative Examples 1-5 were scored according to the sensory scoring standard of peanut protein pie as shown in Table 1. The experimental results are shown in Figure 1. When the peanut protein powder was added too much and the soybean protein powder was too little The protein pie has a loose texture, weak elasticity and hardness; when peanut protein powder: soybean protein powder = 6:3, the protein pie has a good texture and taste; when no gluten is added, the product has increased elasticity and a sweeter flavor. Therefore, discard the gluten, and determine the optimal ratio of peanut protein powder and soybean protein powder, peanut protein: soybean protein = 6:3. In addition, from the data of Comparative Example 5, it can be seen that when the amount of soybean protein powder is greater than that of peanut protein powder, at this time, the elasticity and hardness are moderate, the beany smell is heavy, and subsequent deodorization is required, which increases the complexity of the process. degree, the sensory score decreased. It is fully proved that products with good product quality and high sensory score can be obtained according to the 1.5-2.5:1 ratio of peanut protein powder and soybean protein powder in this application. Product quality and sensory scores for this application.

2、冰水添加量试验2. Ice water addition test

以实施例1为基准,通过改变冰水添加量对蛋白粉进行试验,冰水添加量分别为71%、72%、73%、74%、75%。结果如图2所示:实施例1添加的量为73%,此时获得的产品的质量佳,感官评分最高,在本申请范围内的72%和74%也获得了较佳的产品质量和感官评分,当冰水添加量超出本申请的范围内时,例如当冰水添加量为71%时,物料斩拌不匀,混合物入模困难,产品口感不细腻;水分添加量为75%时,质构松软,产品难成型,成品内部积水较多;因此,冰水的加入量占花生蛋白粉、大豆分离蛋白和水的总质量的72-74%,最适添加量为73%。超出本申请的冰水添加量范围时,产品质量欠佳,感官评分欠佳。Based on Example 1, the protein powder was tested by changing the addition amount of ice water, and the addition amount of ice water was 71%, 72%, 73%, 74%, and 75%, respectively. The results are shown in Figure 2: the amount added in Example 1 is 73%, the quality of the product obtained at this time is good, the sensory score is the highest, and 72% and 74% within the scope of the application also obtained better product quality and Sensory score, when the amount of ice water added exceeds the scope of this application, for example, when the amount of ice water added is 71%, the material will not be chopped and mixed well, the mixture will be difficult to mold, and the product taste will not be delicate; when the amount of water added is 75% , the texture is soft, the product is difficult to form, and there is a lot of water in the finished product; therefore, the amount of ice water added accounts for 72-74% of the total mass of peanut protein powder, soybean protein isolate and water, and the optimum addition amount is 73%. When the amount of ice water added in the application is exceeded, the product quality is not good, and the sensory score is not good.

3、亚麻籽粉添加量试验3. Flaxseed powder addition test

以实施例1为基准,通过改变加入的亚麻籽粉的质量百分数,分别添加3%、5%、7%、9%、11%的粉末,此外,本申请还将实施例1中3%添加量的亚麻籽粉替换为变性淀粉。实验结果如图3和图4所示。从图3可以看出,实施例1添加的亚麻籽粉的质量百分数为3%,亚麻籽粉与水混合后可形成黏稠的液体,具有黏连的作用,可替代传统的粘合类食品添加剂,同时可以赋予蛋白派亚麻籽的风味;此时获得的产品的质量佳,感官评分最高,在本申请范围内的5%也获得了较佳的产品质量和感官评分,当亚麻籽粉添加量超出本申请的范围内时,过度添加亚麻籽粉不会增加产品黏结性,反而会破坏蛋白派弹性和细腻口感。因此,本申请中亚麻籽粉添加量为占花生蛋白粉、大豆分离蛋白、水、植物油和亚麻籽粉的总质量的2-5%,当亚麻籽粉添加量为3%时,产品的感官最佳。超出本申请的亚麻籽粉添加量范围时,产品质量欠佳,感官评分欠佳。此外,本申请选择变性淀粉作为粘合剂时,其最终获得的产品质量和感官评分显著低于实施例1。从图4可以看出,仅采用3%变性淀粉作为粘合剂时,产品质构松软,延长加工时间得到少许改善,但口感依然差;当采用3%亚麻籽粉作为粘合剂时,蛋白派的弹性和硬度增加,咀嚼性较好,当加工时间太长,蛋白派表面水分流失较多,口感减弱。综上,亚麻籽粉作为食品黏合剂效果更佳。Based on Example 1, by changing the mass percentage of the added flaxseed powder, 3%, 5%, 7%, 9%, and 11% of powder were added respectively. In addition, the application also added 3% in Example 1. amount of flaxseed meal replaced with modified starch. The experimental results are shown in Figures 3 and 4. As can be seen from Figure 3, the mass percentage of the linseed powder added in Example 1 is 3%. After the linseed powder is mixed with water, a viscous liquid can be formed, which has the effect of adhesion and can replace traditional adhesive food additives. At the same time, it can give the flavor of flaxseed to protein pie; the quality of the product obtained at this time is good, the sensory score is the highest, and 5% within the scope of the application also obtains better product quality and sensory score, when the amount of flaxseed powder added Beyond the scope of the present application, excessive addition of flaxseed powder will not increase the cohesion of the product, but will destroy the elasticity and delicate taste of the meringue. Therefore, in the present application, the added amount of flaxseed meal is 2-5% of the total mass of peanut protein powder, soybean protein isolate, water, vegetable oil and flaxseed meal. When the added amount of flaxseed meal is 3%, the sensory perception of the product optimal. When the addition amount of flaxseed powder in the present application is exceeded, the product quality is not good, and the sensory score is not good. In addition, when modified starch is selected as the binder in the present application, the final product quality and sensory score obtained are significantly lower than those of Example 1. As can be seen from Figure 4, when only 3% modified starch is used as the binder, the texture of the product is soft, and the processing time is slightly improved, but the taste is still poor; when 3% flaxseed meal is used as the binder, the protein The elasticity and hardness of the pie increase, and the chewiness is better. When the processing time is too long, the surface of the protein pie will lose more moisture and the taste will be weakened. In conclusion, flaxseed meal is more effective as a food binder.

4、谷氨酰胺转胺酶(TG酶)反应条件试验4. Test of transglutaminase (TG enzyme) reaction conditions

以实施例1为基准,通过改变入模加热的参数来获得多组反应条件,其中:第一组为将斩拌物料置于模具中冷藏10h;第二组为将斩拌物料置于模具中50℃恒温反应2h;第三组为将斩拌物料置于模具中50℃恒温反应1h;第四组为将斩拌物料置于模具中55℃恒温反应2h;第五组即为本申请实施例1。Taking Example 1 as a benchmark, multiple sets of reaction conditions were obtained by changing the parameters for heating into the mold, wherein: the first group was to place the chopping material in the mold and refrigerate for 10h; the second group was to place the chopping material in the mold. 50℃ constant temperature reaction for 2h; the third group is to put the chopping material in the mold for 50℃ constant temperature reaction for 1h; the fourth group is to put the chopping material in the mold for 55℃ constant temperature reaction for 2h; the fifth group is the implementation of this application example 1.

由图5结果可得,第二组和第三组在50℃环境下反应2h、1h,产品内部松软不紧致;第一组和第五组感官评分最高,分别为冷藏反应10小时和55℃反应1h,但是,由于冷藏时间较长,不仅严重降低了产品生产速度,同时增加了生产成本和能源损耗。因此,本申请中谷氨酰胺转胺酶(TG酶)反应条件优选为54-56℃环境中放置1-2h,为了节约能源损耗,故优选谷氨酰胺转胺酶反应条件为55℃、1h。超出本申请的反应条件范围时,产品质量欠佳,感官评分欠佳。As can be seen from the results in Figure 5, the second and third groups were reacted at 50 °C for 2h and 1h, and the interior of the product was soft and not firm; the first and fifth groups had the highest sensory scores, refrigerated for 10 hours and 55 respectively. ℃ reaction for 1 h, however, due to the long refrigeration time, not only the production speed of the product is seriously reduced, but also the production cost and energy loss are increased. Therefore, the reaction conditions of transglutaminase (TG enzyme) in this application are preferably placed in an environment of 54-56°C for 1-2 hours. In order to save energy consumption, the reaction conditions of transglutaminase are preferably 55°C for 1 hour. When the reaction conditions of the present application are exceeded, the quality of the product is poor and the sensory score is poor.

5、燕麦添加量试验5. Test of oat addition amount

以实施例1为基准,通过改变实施例1中干燕麦的加入量,分别添加3%、4%、5%、6%、7%的燕麦,干燕麦沸水浸泡30min后沥水获得湿燕麦,最终加入的为湿燕麦。试验结果如图6所示,当燕麦添加量低于6%时,产品无燕麦咀嚼感;燕麦添加量高于6%时,花生蛋白派质构开始松散,因此燕麦添加量为5-7%,最佳添加量为6%。Taking Example 1 as a benchmark, by changing the addition of dry oats in Example 1, 3%, 4%, 5%, 6%, 7% of oats were added respectively, and the dry oats were soaked in boiling water for 30min to drain to obtain wet oats. Added wet oats. The test results are shown in Figure 6. When the oat content is less than 6%, the product has no oat chewiness; when the oat content is higher than 6%, the texture of peanut protein pie begins to loosen, so the oat content is 5-7%. , the optimum addition amount is 6%.

6、烘烤温度与时间试验6. Baking temperature and time test

以实施例1为基准,通过改变实施例1中的烘烤温度和烘烤时间,设置于60℃、70℃、80℃、90℃、100℃的烘烤温度下烘烤20min,同时设计于70℃的烘烤温度下烘烤10min、20min、30min、40min、50min。试验结果由图7、图8可得:烘烤温度过高、时间过长,蛋白派表面水分散失过多,导致产品焦糊,颜色呈现深棕色,感官不佳;烘烤温度较低、时间太短时,未达到封边效果,后续加工过程易破坏蛋白派的形状;综上,确定蛋白派烘烤温度为60-80℃,烘烤10-20min,最佳烘烤温度为70℃、烘烤时间为20min。Taking Example 1 as the benchmark, by changing the baking temperature and baking time in Example 1, set the baking temperature at 60°C, 70°C, 80°C, 90°C, and 100°C for 20 minutes of baking. Bake at 70°C for 10min, 20min, 30min, 40min, 50min. The test results can be obtained from Figure 7 and Figure 8: the baking temperature is too high and the time is too long, and the surface of the meringue loses too much water, resulting in a burnt product, dark brown color, and poor sensory feel; low baking temperature and time If it is too short, the edge sealing effect will not be achieved, and the shape of the meringue pie will be easily damaged during the subsequent processing; Baking time is 20min.

综上所述,本申请提供的富含膳食纤维的花生蛋白派采用花生蛋白粉为主要原料,较传统蛋白凝胶制品,本发明无豆腥味且口感细腻,无需调味、卤制以去腥,也无需使用高水分挤压等设备,节约了能源损耗和生产成本;花生蛋白持水性良好,以花生蛋白粉为主要原料后,进一步减少了水分添加量,降低了冰水制作成本。进一步地,本发明在花生蛋白粉的基础上,添加了大豆分离蛋白粉、燕麦和亚麻籽粉。花生蛋白粉口感清甜、持水性良好,但花生蛋白的赖氨酸、苏氨酸、蛋氨酸含量较低,凝胶性较弱;大豆分离蛋白粉、燕麦和亚麻籽粉这三种辅料平衡了花生蛋白的氨基酸缺陷,三者凝胶性能均良好,提升了产品质构。同时亚麻籽粉还可以替代了变性淀粉等粘合类食品添加剂,清洁了产品标签、丰富了蛋白派的营养价值。本发明在原料斩拌后,将混合物置于55℃环境中静置1h即可达到良好的硬度和弹性,其他蛋白类凝胶产品至少需要反应2h,多则需要10h以上。因此,本发明增强了工艺连续性、节约了能源损耗,提升了生产速度。To sum up, the peanut protein pie rich in dietary fiber provided by the present application uses peanut protein powder as the main raw material. Compared with traditional protein gel products, the present invention has no beany smell and has a finer mouthfeel, and does not need to be seasoned and marinated to remove fishy smell. There is also no need to use equipment such as high-moisture extrusion, which saves energy loss and production costs; peanut protein has good water holding capacity, and using peanut protein powder as the main raw material further reduces the amount of water added and the production cost of ice water. Further, the present invention adds soybean protein isolate powder, oat and flaxseed powder on the basis of peanut protein powder. Peanut protein powder has a sweet taste and good water retention, but the content of lysine, threonine, and methionine in peanut protein is low, and the gelling property is weak; soy protein isolate powder, oat and flaxseed powder are balanced by three accessories Due to the amino acid deficiency of peanut protein, the gel properties of the three are good, which improves the product texture. At the same time, flaxseed meal can also replace adhesive food additives such as modified starch, which cleans product labels and enriches the nutritional value of protein pie. In the present invention, after the raw materials are chopped and stirred, the mixture is placed in a 55°C environment for 1 hour to achieve good hardness and elasticity. Other protein gel products require at least 2 hours of reaction, and more than 10 hours. Therefore, the present invention enhances process continuity, saves energy consumption, and increases production speed.

以上所述仅为本发明的优选实施例而已,并不用于限制本发明,对于本领域的技术人员来说,本发明可以有各种更改和变化。凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims (10)

1. The peanut protein pie rich in dietary fiber is characterized by comprising the following raw materials in parts by weight: 10-15 parts of peanut protein powder, 5-7 parts of soybean protein isolate, 45-50 parts of water, 10-15 parts of vegetable oil, 1-3 parts of flaxseed powder, 0.3-1 part of transglutaminase and 7-9 parts of oat.
2. The dietary fiber-rich peanut protein pie as claimed in claim 1, wherein the mass ratio of the peanut protein powder to the soybean protein isolate is 1.5-2.5: 1.
3. dietary fiber-rich peanut protein pie according to claim 1, characterized in that the water is added in an amount of 72-74%, preferably 73% of the total mass of the peanut protein powder, the soy protein isolate and the water.
4. A dietary fiber-rich peanut protein pie according to claim 1, wherein the flaxseed meal is added in an amount of 2-5%, preferably 3% of the total mass of the peanut protein meal, the soy protein isolate, the water, the vegetable oil and the flaxseed meal.
5. The dietary fiber-rich peanut protein pie according to claim 1, wherein the oats are prepared by soaking dried oats in boiling water for 20-40min and draining, and the mass ratio of the oats to the dried oats is 7-9: 5.
6. Dietary fiber-rich peanut protein pie according to claim 5, characterized in that the dry oats are added in an amount of 5-7%, preferably 6% of the total mass of the peanut protein meal, the soy protein isolate, the water, the vegetable oil, the linseed meal and the transglutaminase.
7. Dietary fiber-rich peanut protein pie according to claim 1, characterized in that the vegetable oil is at least one of peanut oil, soybean oil, rapeseed oil, sesame oil, cottonseed oil, rice bran oil, corn oil, sunflower seed oil and linseed oil; preferably, the vegetable oil is peanut oil.
8. A method for preparing a peanut protein pie rich in dietary fiber, which comprises mixing the raw materials of the peanut protein pie rich in dietary fiber according to any one of claims 1 to 7 to obtain a chopped material, feeding the chopped material into a mold for heating, demolding and baking to obtain the peanut protein pie.
9. The method for preparing a dietary fiber-rich peanut protein pie according to claim 8, wherein mixing the raw materials comprises: chopping and mixing the peanut protein powder, the soybean protein isolate and the water uniformly at the stirring speed of 3000-3500r/min, and chopping and mixing for 4-6min at the stirring speed of 3000-3500 r/min; then adding the vegetable oil, and chopping for 2-4min at the stirring speed of 3000-3500 r/min; then adding the linseed powder, the transglutaminase and the oat, and continuously chopping for 1-2min at the stirring speed of 3000-3500r/min to obtain a chopped material;
preferably, the mold-in heating comprises: placing the chopped and mixed material in a mould and placing for 1-2h at the temperature of 54-56 ℃;
preferably, the demolding bake comprises: cutting the blank demoulded from the mould into blocks, and baking for 10-20min at the temperature of 60-80 ℃;
preferably, the blank is turned over 1-2 times during baking.
10. The method for preparing a dietary fiber-enriched peanut protein pie according to claim 8, further comprising a packaging sterilization step after the de-molding baking step: cooling the product to room temperature after baking, vacuumizing, and sterilizing for 25-35min at the temperature of 110-120 ℃ and the pressure of 0.15-0.2 MPa.
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